JPH039421B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood test container, and more particularly to a bottomed tubular container, a so-called Spitz, used to separate serum from a whole blood sample of a blood recipient by centrifugation. In recent years, coupled with the remarkable progress in inspection technology,
Blood tests such as serum biochemical tests, serum immunological tests, and hematology tests have become widely used, and have come to greatly contribute to disease prevention and early diagnosis. Serum tests are the main body of blood tests, and the serum required for tests is usually obtained by coagulating blood collected in a blood test container and then centrifuging it to form blood clots with different specific gravities (fibrin and blood cells). separated from the mixed gel-like mass). Conventional blood test containers are made of glass and synthetic resins such as polystyrene, polymethyl methacrylate, and polyethylene. However, conventional blood test containers have the disadvantage that it takes a considerable amount of time for blood to coagulate after it is injected into the container, making it impossible to conduct tests quickly. This was a problem when it needed to be done. It is a big problem that it takes time for blood to coagulate even in the blood of normal healthy people, but patients undergoing artificial dialysis or patients with thrombosis are given heparin to prevent blood clots. The blood of such patients contains a considerable concentration of heparin, making it difficult to separate and collect serum during clinical tests because blood coagulation is difficult to occur. The object of the present invention is to obtain a blood test container that has the same effect of promoting blood coagulation on such patient's blood as that on normal healthy people and has excellent serum separation properties. The gist of the present invention is as follows: 1. Having at least one group selected from the group consisting of a quaternary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, which has an anion exchange ability, on the inner wall surface. Anion exchange resin consisting of a water-insoluble polymer and having a total ion exchange capacity in the range of 2.5 to 6.0 milliion equivalents per gram of dry ion exchange resin, in the range of 5 mg to 500 mg per 10 c.c. of blood volume. A blood test container, characterized in that: (a) a fourth container having an anion exchange capacity is present on the inner wall surface;
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. 2.5 or so per gram
An anion exchange resin in the range of 6.0 milliion equivalents and (b) selected from the group consisting of glass, silica, kaolin, celite and bentonite, with a particle size of 50 μm or less and an average particle size of 10 μm or less, Linseed oil absorption amount is 20~40ml/
100g, BET specific surface area value is 5000-30000cm ² /g,
An adsorbent inorganic substance having a specific resistance value of 1×10 ¹⁰ Ω・cm or less is mixed with the proportion of (a) and (b) of 1 part by weight: 0.001
present in the range of 10 to 10 parts by weight, and (a)
3. A container for blood testing, characterized in that a container for blood testing is present in a range of 5 mg to 500 mg per 10 c.c. of blood volume.
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. 2.5 or so per gram
An anion exchange resin in the range of 6.0 million ion equivalents and (b) 2,2',4,4'-tetrahydroxybenzophenone, the ratio of (a) and (b) being 1 part by weight:
A blood test container, characterized in that the amount of (a) is present in the range of 0.0005 to 0.5 parts by weight, and the amount of (a) is in the range of 5 mg to 500 mg per 10 c.c. of blood volume. On the wall surface, (a) a fourth layer having anion exchange ability;
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. 2.5 or so per gram
An anion exchange resin and (b) ellagic acid having a range of 6.0 million ion equivalents are present in a ratio of (a) and (b) in the range of 1 part by weight: 0.0005 to 0.5 part by weight, and ( b) 5 mg or more per 10 c.c. of blood volume
A container for blood testing, characterized in that the amount of the blood test container is present in the range of 500 mg.5.
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. 2.5 or so per gram
Anion exchange resin and (b) epicatechin in the range of 6.0 million ion equivalents, the ratio of (a) and (b) is 1.
Part by weight: present within the range of 0.0005 to 0.5 part by weight, and the amount of (a) is 5 mg or more per 10 c.c. of blood volume.
A container for a blood test, characterized in that the amount of blood is present in a range of 500 mg. Next, the blood test container of the present invention will be explained in more detail. In the present invention, any of thermoplastic resins, thermosetting resins, modified natural resins, and glass can be used as the material for the blood test container, that is, the container. Examples of thermoplastic resins include polyethylene, polypropylene, poly-4-methylpentene-1, polystyrene, polymethyl methacrylate, polyvinyl chloride, polyethylene terephthalate, polybutylene terephthalate, styrene-acrylonitrile copolymer, and styrene-butadiene copolymer. , styrene-isoprene copolymer, styrene-maleic anhydride copolymer, styrene-acrylic acid copolymer, styrene-methyl methacrylate copolymer, ethylene-propylene copolymer, ethylene-acrylic acid copolymer, ethylene- Acrylic ester copolymers, polyvinyl alcohol acetals, polyvinyl alcohol butyrals, etc., and as thermosetting resins, for example, unsaturated polyester resins, epoxy resins, epoxy-acrylate resins, etc. are used. As the modified natural resin, cellulose acetate, cellulose propionate, cellulose acetate butyrate, ethyl cellulose, ethyl chitin, etc. are used. As the glass, silicate glasses such as soda lime glass, phosphosilicate glass, and borosilicate glass, and quartz glass are used. In the blood test container of the present invention, an anion exchange resin is present on the inner wall surface. The anion exchange resin is a water-insoluble polymer having anion exchange ability and having at least one group selected from the group consisting of a quaternary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group. used. Examples of the anion exchange resin having a quaternary ammonium group include a modified styrene/divinylbenzene crosslinked copolymer having a trimethylbenzylammonium side chain or a dimethylhydroxyethylbenzylammonium side chain, and a modified product having an N-methylpyridine side chain. Modified copolymers of vinylpyridine and styrene or methyl methacrylate, cellulose derivatives having triethylaminoethyl side chains, N-triethyl chitosan, and the like are used. In these anion exchange resins having a quaternary ammonium group, the quaternary ammonium group exhibits cationic properties and therefore always bonds with a counter ion, which is an anion. As a counterion, F ^- ,
It may be any of halogen ions such as Cl ^- , Br ^- , I ^-, or OH ^- . Examples of anion exchange resins having a tertiary amino group include modified styrene/divinylbenzene crosslinked copolymers having dimethylbenzylamine side chains, acrylamide and divinylbenzene having N-3-dimethylaminotrimethyleneamide side chains; Crosslinked copolymers, copolymers of vinyl pyridine and styrene or methyl methacrylate, cellulose derivatives having diethylaminoethyl side chains, N-diethyl chitosan, and the like are used. As the anion exchange resin having a secondary amino group, for example, a modified styrene/divinylbenzene crosslinked copolymer having a methylbenzylamine side chain, monoethylaminoethyl cellulose, etc. are used. Examples of the anion exchange resin having a primary amino group include a modified styrene/divinylbenzene crosslinked copolymer having a benzylamine side chain, aminoethyl cellulose, P-aminobenzyl cellulose,
Chitosan etc. are used. The anion exchange resin used in the present invention may also have a mixed type of primary, secondary, and tertiary amino exchange group. Examples of such materials include modified styrene-divinylbenzene crosslinked copolymers having N-diethylaminobenzylamine side chains, guanidoethylcellulose, and the like. The anion exchange resin used in the present invention has a total ion exchange capacity of 2.5 to 6.0 milliion equivalents per gram of dry ion exchange resin, and particularly preferably 3.5 to 4.5 milliion equivalents. It has a quaternary ammonium group in the million ion equivalent range. Furthermore, when a bromide ion is present as a counter ion to a quaternary ammonium group, compared to the case where other types of counter ions such as chloride ions are used,
It was found that stable performance was obtained. In other words, it was found that bromide ion as a counter ion has the effect of suppressing the destructive effect of ammonium groups on blood components, such as hemolytic effect, and does not impair the effect of efficiently supplementing heparin in the blood. When the anion exchange resin present on the inner wall of the blood test container comes into contact with blood containing heparin, it quickly eliminates the action of heparin and restores the normal coagulation function of the blood, thereby improving blood test results. The blood in the container is coagulated within a short period of time, and after the coagulation is completed, the blood clot and serum are separated by means such as centrifugation, whereby the serum can be easily collected. To explain this point in more detail, when normal blood comes into contact with the inner wall surface of the blood container, activation of factor factor among the coagulation factors immediately progresses, and this becomes the starting point for coagulation to occur in a chain reaction. Eventually, thrombin generated by activation of prothrombin acts on fibrinogen to form an insoluble fibrin network, and coagulation is completed. On the other hand, in blood to which heparin has been added, heparin acts cooperatively with antithrombin present in the blood, significantly inhibiting the action of thrombin. It is said that heparin not only inhibits the action of thrombin but also inhibits the action of other coagulation factors including factor factor. Therefore, by normal means, fibrinogen does not convert to fibrin in heparin-containing blood, and serum cannot be separated and collected because coagulation does not occur. It is thought that 1 to 10 units of heparin are present per 10 c.c. of blood in the blood of artificial dialysis patients or thrombosis patients receiving heparin administration. When such heparin-containing blood is placed in a blood test container having an anion exchange resin according to the present invention on its inner wall, heparin is adsorbed by the anion exchange resin on the inner wall and removed from the blood. As a result, thrombin and other blood coagulation factors regain their normal functions. In the present invention, an anion exchange resin is present on the inner wall surface of a blood test container in an amount ranging from 5 mg to 500 mg per 10 c.c. of blood volume. If the amount is less than this range, the effect of adsorbing heparin will be insufficient and sufficient blood coagulation will not be achieved. Moreover, if it exceeds this range, abnormal values may appear in clinical test values such as serum biochemical tests. The anion exchange resin in the present invention can be in any shape, including granules, sheets, and films. In the case of granular materials, those having a particle size of 20 microns to 5 mm can be used. In the above case, when the anion exchange resin is present alone on the inner wall surface of the container, a significant blood coagulation promoting effect is observed. However, it is possible to further improve the blood coagulation promoting effect by using an anion exchange resin together with an adsorbent inorganic substance, 2,2',4,4'-tetrahydroxybenzophenone, ellagic acid, or epicatechin. can. One of the substances that synergistically exhibits a blood coagulation promoting effect when used in combination with an anion exchange resin is an adsorbent inorganic substance. Adsorptive inorganic substances include inorganic substances used as adsorbents, such as glass, silica,
Selected from water-insoluble inorganic fine powders such as kaolin, celite, and bennite. Further, the adsorbent inorganic substance used has a particle size of 50 μm or less, and an average particle size of 10 μm or less. In particular, silica is an adsorbent inorganic material that is effective in shortening blood coagulation time.
Porous silica containing 20% by weight or more exhibits excellent effects. Such adsorbent inorganic substances have the effect of promoting the activation of blood coagulation factors and the aggregation of platelets when they come into contact with blood. However, in order for the adsorbent inorganic substance to effectively exhibit a blood coagulation promoting effect, it is preferable that the linseed oil absorption amount, BET specific surface area value, and specific resistance value exist within certain ranges. The linseed oil absorption amount and BET specific surface area value represent the degree of surface area of the adsorbent inorganic material, and since the surface area is related to the degree of surface pores of the adsorbent inorganic material, the surface pores are determined by the oil absorption amount and specific surface area. You can know the extent of The adsorbent inorganic material in the present invention has a linseed oil absorption of 20 to 40 ml/100 g,
Those having a BET specific surface area value of 5,000 to 30,000 cm ² /g are used. Linseed oil absorption is Japanese Industrial Standard K
−5101. The BET specific surface area value is calculated by calculating the amount of gas that covers the surface as a monomolecular layer from the amount of gas adsorbed on the surface of the adsorbent inorganic material, the equilibrium pressure at that time, and the saturated vapor pressure of the adsorbed gas, and then calculating the amount of gas that covers the surface as a monomolecular layer. It refers to the value calculated by multiplying by the average cross-sectional area of , and nitrogen gas, oxygen gas, argon gas, methane gas, etc. are used as the adsorption gas. And according to this method,
Surface area values including pores that cannot be measured by measuring linseed oil absorption are measured. During blood coagulation, factor factor, or contact factor, is activated, and for this purpose three factors, factor factor, prekallikrein, and polymeric kininogen, are deposited on the surface of a foreign body.
It is necessary for the seed substances to form a complex and be adsorbed, and it is said that adsorption on a membrane lacking one or two of these substances will not lead to activation. By the way, when an adsorbent inorganic substance is used with the expectation of promoting blood coagulation, if the surface area is very large, factor factor, prekallikrein, and The adsorption rate of high-molecular-weight kininogen will increase, in other words, the rate of complex formation between the three components necessary for activation of factor factor will decrease, and the blood coagulation promoting effect will be reduced. . On the other hand, if the surface area of the adsorbent inorganic material is too small, the probability of adsorption of coagulation factors will be low, making it impossible to expect a blood coagulation promoting effect. For this reason, the adsorbent inorganic material in the present invention has a linseed oil absorption of 20 to 40 ml/100 g and a BET specific surface area of 5000.
Those having a surface area in the range ˜30000 cm ² /g are used. Further, in the present invention, the adsorptive inorganic material has a specific resistance value of 1×10 ¹⁰ Ω·cm or less, and most preferably 5×10 ⁴ Ω·cm or less. The specific resistance value is the reciprocal of electrical conductivity, and is the value at room temperature. When blood comes into contact with a foreign object, proteins such as albumin, globulin, and various blood coagulation factors immediately adsorb to the surface of the foreign object prior to blood coagulation, and changes in the conformation of protein molecules occur during this time.
There are various effects on the subsequent biochemical reactions. In particular, the activation mechanism of blood coagulation factors, which is an enzymatic reaction, is greatly affected, and in some cases, the coagulation function is impaired. In addition, platelets adhering to adsorbed globulin and albumin that have undergone a large conformation change undergo abnormal melting and deformation, causing a phenomenon in which polymerized and precipitated fibrin chains strongly adhere to adsorbent inorganic substances. If the latter phenomenon occurs in a container intended for serum collection, even if centrifugation is performed, blood clots and serum will not be separated, making it impossible to achieve the purpose. Changes in protein conformation occur as a result of various interactions between adsorbent inorganics and proteins, such as hydrophobic interactions, hydrogen bonding interactions, and electrostatic interactions. This can be alleviated by using an adsorptive inorganic substance with relatively high conductivity. In other words, the polar groups of proteins induce a dipole moment group in the adsorbent inorganic substance with a corresponding distribution, but if the adsorbent inorganic substance is nonconductive, it is conductive The response becomes worse than that of the protein adsorbed on the surface, and the potential distribution of the protein adsorbed on the surface and the potential distribution of the adsorbing inorganic substance are inconsistent with each other, which causes local and non-uniform distortion of the protein. This leads to changes in conformation. Therefore, it is necessary for the adsorptive inorganic substance to have conductivity in order to maintain consistency in potential distribution between the protein and the adsorbent inorganic substance and to prevent changes in protein conformation. For this reason, the adsorptive inorganic substance in the present invention has a specific resistance value of 1×10 ¹⁰ Ω·cm or less. Adsorptive inorganic substances have a blood coagulation promoting effect by activating blood coagulation factors.
However, when an adsorbent inorganic substance is present alone on the inner wall surface of a container, although the rate of blood coagulation is accelerated, it has the function of causing the blood produced by coagulation to adhere to the inside of the container, and even when subjected to centrifugation, the coagulated blood remains. Sometimes it is difficult to separate serum and blood clots, and during centrifugation, the strong shear stress generated between blood clots and adsorbent inorganic substances on the inner wall of the container destroys red blood beads and dissolves them into serum. Sometimes I put it away. However, when an anion exchange resin and an adsorbent inorganic substance are present on the inner wall of the container, it is possible to prevent blood clots from adhering to the inner wall of the container and prevent hemolysis into serum during centrifugation. The ratio of the anion exchange resin and adsorbent inorganic substance used is within the range of 0.001 to 10 parts by weight of the adsorbent inorganic substance per 1 part by weight of the anion exchange resin. Other substances that synergistically exhibit a blood coagulation promoting effect when used in combination with anion exchange resins are 2, 2', 4,
4'-tetrahydroxybenzophenone. 2,2,',4,4'-tetrahydroxybenzophenone has the following chemical structural formula. 2,2',4,4'-tetrahydroxybenzophenone has a melting point of 195℃ and a solubility in solvents of 30℃.
0.1% by weight for water, 50% by weight for methanol, 40% by weight for ethanol, and 10% by weight for a 1:1 water-ethanol solution. Further, the maximum absorption wavelength position is 345 mμ, and the color value (Gardner) is No. 8 in a 1% by weight methanol solution. The ratio of anion exchange resin and 2,2',4,4'-tetrahydroxybenzophenone is 2,2',4,4'-tetrahydroxybenzophenone per 1 part by weight of anion exchange resin. It is within the range of 0.0005 to 0.5 parts by weight. Another substance that exhibits a synergistic blood coagulation promoting effect when used in combination with an anion exchange resin is ellagic acid. Elazinic acid is a substance with the following chemical structural formula. Ellagic acid is known as a substance that activates factor factor, which is one of the blood coagulation factors.
It was confirmed that when used in combination with an anion exchange resin, it has a more excellent blood coagulation promoting effect than when used alone. The ratio of ellagic acid to the anion exchange resin is 0.0005 to 0.5 per 1 part by weight of the anion exchange resin.
It is considered to be within the range of parts by weight. Yet another substance that exhibits a synergistic blood coagulation promoting effect when used in combination with an anion exchange resin is epicatechin. Epicatechin is a substance with the following chemical structural formula. Epicatechin is a colorless columnar crystal with a decomposition temperature of 237
~238℃, the maximum absorption wavelength position is 282mμ, and it is a substance that is hardly soluble in water. The ratio of the anion exchange resin and epicatechin used is within the range of 0.0005 to 0.5 parts by weight of epicatechin per 1 part by weight of the anion exchange resin. anion exchange resin or an adsorbent inorganic substance,
In order to have 2,2',4,4'-tetrahydroxybenzophenone, ellagic acid, or epicatechin present on the inner wall surface of the container, use a diluent in which these are dissolved or suspended. Preferred methods include applying a liquid to the inner wall surface and then drying the solvent to form a film, and applying a diluted liquid as a spray liquid. Suitable media after dilution include alcohols such as methanol, ethanol, and propyl alcohol, water, and halogenated hydrocarbons such as chloroform, dichloroethylene, monochlorotrifluoroethylene, and dichlorodifluoromethane. In addition, water-soluble polymers such as polyvinylpyrrolidone, polyvinyl alcohol, methylcellulose, hydroxypropylcellulose, hydroxyethyl methacrylate, or sorbitan monostearate, glycerin monostearate are added to the diluted solution in which each of the above components is dissolved or suspended. Adding surfactants such as
It is effective in binding the anion exchange resin to the container wall. According to the blood test container of the present invention, it not only has an excellent blood coagulation promoting effect on the serum of normal healthy people, but also has an excellent effect on blood coagulation promoting effect on serum of normal healthy people. Therefore, even in the case of blood that is difficult to coagulate, it has an excellent blood coagulation promoting effect, and serum and blood clots can be easily separated. The blood test container of the present invention includes blood test blood collection tubes,
It can be suitably used for applications such as blood sampling syringes and serum separation containers. Example 1 A modified styrene/divinylbenzene crosslinked copolymer having trimethylbenzylammonium side chains was used on the inner wall of a bottomed glass tube with an outer diameter of 15 mm, an inner diameter of 13 mm, and a height of 100 mm from the bottom to 50 mm. A 400-mesh granular anion exchange resin (8 mg) was uniformly applied. The anion exchange resin used here was a chlorine salt type with chlorine ions as counter ions, and had an ion exchange capacity of 3.5 milliequivalents per 1 gr of dry weight. The anion exchange resin was applied to the inner wall of the container using a mixture of 10 gr of ethyl alcohol, 5 gr of the anion exchange resin, and 1 gr of polyvinylpyrrolidone, and after application, the ethyl alcohol was completely evaporated to dryness. This glass container contains 5ml of heparin blood.
After injecting 5 ml of fresh human blood containing 5 units per tube, the blood coagulation property was evaluated by standing at 20°C and measuring the time required for the whole blood to stop flowing completely as the blood coagulation time. Immediately after blood coagulation, centrifugation was performed for 5 minutes at a rotation speed of 3,000 rpm to observe the state of serum separation, and the ascending serum was collected with a pipette, and the amount was taken as the serum yield. The state of serum separation was evaluated by the presence or absence of residual blood clot adhesion on the vessel wall in the serum layer and the presence or absence of fibrin network precipitation. As is clear from the results in the Example 1 column of Table 1, the blood test container of the present invention had particularly excellent blood coagulation properties for heparin-containing blood. In addition, in order to examine whether the presence of the anion exchange resin affected blood test values, a serum biochemical test (28 items) was conducted using the serum obtained from the blood test container. A significant difference test was conducted on the test values between the glass tube not using the anion exchange resin and the blood test container of the present invention (n=5), and the presence or absence of a difference at a significance level of 5% was tested. As a result, it was confirmed that when used in clinical tests, it did not affect test values at all. Example 2 In Example 1, the same blood test container as in Example 1 was used, except that the anion exchange resin had bromide ions as counter ions. This anion exchange resin is
It had an ion exchange capacity of 3.5 meq. The blood coagulation properties, serum separation state, and serum yield for heparin-containing blood using this blood test container are as shown in the column of Example 2 in Table 1. A significantly improving effect was observed, and therefore the serum yield was also increased. Furthermore, no effects on clinical test values were observed. Example 3 Modified styrene/divinylbenzene crosslinked copolymer having trimethylbenzylammonium side chains with bromine counter ions on the inner wall of a polyethylene tube with an outer diameter of 15 mm, an inner diameter of 13 mm, and a height of 100 mm from the bottom to 50 mm. 400 mesh granular anion exchange resin (ion exchange capacity is dry weight
3.5 milliequivalents per 1 gr) and natural amorphous assilicic acid fine powder (average particle size 2 microns, linseed oil absorption 30 ml/100 g, BET specific surface area value 1.5 × 10 ⁴ /g, specific resistance value 2.6 × 10 ⁴ Ω・cm) and polyvinylpyrrolidone were mixed and applied. The coating solution was prepared by dissolving and dispersing 5g of the above anion exchange resin, 1g of the adsorbent inorganic material, and 1g of polyvinylpyrrolidone in 100g of ethyl alcohol. After applying this to the inner surface of the polyethylene tube, the ethyl alcohol was evaporated. Dry. The coating amount per blood test container thus obtained was 7 mg of the anion exchange resin and 0.4 mg of the adsorbent inorganic substance. Next, using this blood test container, Example 1
The blood coagulability, serum separation state, and serum yield of heparin-containing blood were evaluated in the same manner as above. The results are as shown in the column of Example 3 in Table 1, and the coagulation of heparin-containing blood was significantly promoted, and the serum separation state was also very good. Examples 4 to 6 In Example 3, 2,
A blood test sample was prepared in the same manner as in Example 3, except that 2',4,4'-tetrahydroxybenzophenone (Example 4), ellagic acid (Example 5), and epicatechin (Example 6) were used. Got the container. The amount of coating on the inner wall surface of these blood test containers is 2.
2′,4,4′-tetrahydroxybenzophenone
0.25mg, ellagic acid 0.17mg, epicatechin
It was 0.20 mg. Next, using these blood test containers, blood coagulation properties for heparin-containing blood, serum separation state, and serum yield were evaluated in the same manner as in Example 1. The results are shown in the columns of Examples 4 to 6 in Table 1. When 2,2',4,4'-tetrahydroxybenzophenone, ellagic acid, or epicatechin is used as a substance to be applied in combination with the anion exchange resin, it significantly accelerates the coagulation of heparin-containing blood and also reduces serum separation. It was in extremely good condition. Comparative Examples 1-2 Same glass tube as used in Example 1 without coating with anion exchange resin (Comparative Example 1)
The same polyethylene tube as used in Examples 3 to 6 but not coated with the anion exchange resin (Comparative Example 2) was prepared, and serum coagulation for heparin-containing blood was performed under the same conditions as in Examples 1 to 6. The results of evaluating the properties are shown in the columns of Comparative Examples 1 and 2 in Table 1. In both cases, after being left for 10 hours, blood coagulation was still not completed and serum separation was impossible. 【table】

Claims (1)

[Scope of Claims] 1. Having at least one group selected from the group consisting of a quaternary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, which has an anion exchange ability, on the inner wall surface. Consisting of a water-insoluble polymer,
An anion exchange resin with a total ion exchange capacity of 2.5 to 6.0 milliion equivalents per gram of dry ion exchange resin is present in a range of 5 mg to 500 mg per 10 c.c. of blood volume. A blood test container characterized by: 2 On the inner wall surface, (a) a fourth layer having anion exchange ability
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. An anion exchange resin having a weight range of 2.5 to 6.0 milliion equivalent per gram and (b) an anion exchange resin selected from the group consisting of glass, silica, kaolin, celite and bentonite, with a particle size of 50 μm or less and an average particle size of It is 10 μm or less, has a linseed oil absorption of 20 to 40 ml/100 g, a BET specific surface area of 5000 to 30000 cm ² /g, and a specific resistance of 1×10 ¹⁰
An adsorbent inorganic substance having an adsorption capacity of Ω cm or less is present in a ratio of (a) and (b) in the range of 1 part by weight: 0.001 to 10 parts by weight, and (a) is added to a blood volume of 10 c. A blood test container characterized in that the amount of .c. is present in a range of 5 mg to 500 mg. 3 On the inner wall surface, (a) a fourth layer having anion exchange ability
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. anion exchange resin in the range of 2.5 to 6.0 milliion equivalents per gram; and (b) 2.
2',4,4'-tetrahydroxybenzophenone, the ratio of (a) and (b) being 1 part by weight: 0.0005 to
A blood test container, characterized in that (a) is present in an amount of 0.5 parts by weight, and in an amount of 5 mg to 500 mg per 10 c.c. of blood volume. 4 On the inner wall surface, (a) a fourth layer having anion exchange ability
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. Anion exchange resin and (b) ellagic acid in the range of 2.5 to 6.0 milliion equivalent per gram, with the ratio of (a) and (b) in the range of 1 part by weight: 0.0005 to 0.5 part by weight. A container for blood testing, characterized in that (a) is present in a range of 5 mg to 500 mg per 10 c.c. of blood volume. 5 On the inner wall surface, (a) a fourth layer having anion exchange ability
It is composed of a water-insoluble polymer having at least one group selected from the group consisting of a primary ammonium group, a tertiary amino group, a secondary amino group, and a primary amino group, and has a total ion exchange capacity of ion exchange resin in a dry state. Anion exchange resin and (b) epicatechin in the range of 2.5 to 6.0 milliion equivalents per gram, the ratio of (a) and (b) being 1 part by weight: 0.0005
present in the range of 0.5 parts by weight, and (a)
A container for blood testing, characterized in that the amount of is present in a range of 5 mg to 500 mg per 10 c.c. of blood volume.