JPH0372864A - Apparatus for breaking bubble in vessel - Google Patents
Apparatus for breaking bubble in vesselInfo
- Publication number
- JPH0372864A JPH0372864A JP20780589A JP20780589A JPH0372864A JP H0372864 A JPH0372864 A JP H0372864A JP 20780589 A JP20780589 A JP 20780589A JP 20780589 A JP20780589 A JP 20780589A JP H0372864 A JPH0372864 A JP H0372864A
- Authority
- JP
- Japan
- Prior art keywords
- tank
- net
- vessel
- bubbles
- bubble breaking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000012510 hollow fiber Substances 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 9
- 229920002545 silicone oil Polymers 0.000 claims description 8
- 239000004519 grease Substances 0.000 abstract description 16
- 229920001296 polysiloxane Polymers 0.000 abstract description 12
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 3
- 210000002445 nipple Anatomy 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 7
- 239000002518 antifoaming agent Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000003254 anti-foaming effect Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 230000007774 longterm Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Degasification And Air Bubble Elimination (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、発泡を伴う槽内化学反応又は槽内培養におけ
る槽内破泡装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an in-tank bubble breaking device for in-tank chemical reactions or in-tank culture involving foaming.
従来の消泡の方法としては、消池剤を培養液中に投入し
ていたが、最近、培養槽中に消泡層を設けることを特徴
とする特開昭６４−６７１８４号に記載の培養方法が考
案された。上記公報では、長期にわたる連結培養（２か
月以上）について記載はなく、また該公報のような消泡
層を構成するものでは、長期的な培養については、破泡
効果が減少してくるものであった。それは培養液の初期
に消泡層を形成するＳＵＳ製の格子状の網状のものにシ
リコングリスを塗布したものであるため、培養時間の経
過とともにシリコングリスが消費され、破泡能力が消滅
してしまうからである。The conventional antifoaming method was to put an antifoaming agent into the culture solution, but recently, a culture method described in JP-A No. 64-67184, which is characterized by providing an antifoaming layer in the culture tank, has been developed. A method was devised. In the above publication, there is no mention of long-term connected culture (more than 2 months), and in the case of products that constitute a defoaming layer as in the publication, the foam-breaking effect decreases with long-term culture. Met. Because silicone grease is applied to a SUS lattice-like mesh that forms an anti-foaming layer in the early stage of the culture solution, the silicone grease is consumed as the culture time passes and the foam-breaking ability disappears. This is because it will be put away.
上記従来技術は、消泡ネット表面にシリコン系油脂が塗
布されているだけで、使用中に、塗布したシリコングリ
ス又は、シリコンオイルが消費され、数か月オーダーの
長期にわたり連続的に用いる場合には適していなかった
。In the above conventional technology, the antifoaming net surface is simply coated with silicone oil and fat, but the applied silicone grease or silicone oil is consumed during use, and when used continuously for a long period of time on the order of several months. was not suitable.
本発明の目的は、培養等比較的長期間にわたっての使用
される装置の槽内破泡装置を提供することにある。An object of the present invention is to provide a bubble-busting device for a device used for relatively long periods of time, such as culture.
上記目的を達成するために、疎水性の中空糸膜な用い、
該中空糸膜な３〜４ｍ間隔で液面の上部に格子状又は平
面状にはりめぐらせ、槽外部より該形成された中空糸膜
中にシリコンオイル又はシリコングリスを注入するよう
に構成したものである。In order to achieve the above purpose, a hydrophobic hollow fiber membrane is used,
The hollow fiber membranes are arranged in a grid or planar manner above the liquid surface at intervals of 3 to 4 m, and silicone oil or silicone grease is injected into the formed hollow fiber membranes from outside the tank. It is.
槽外部に設けられた注入口よりシリコングリス、又はシ
リコンオイルをシリンジを用いて中空糸膜内に注入する
。該注入により液面上部に張りめぐって形成されている
中空糸Ｍ表面に一様に注入した溶液が滲み出てくる。槽
内液より生じた泡は、中空糸膜表面のシリコングリス、
又はシリコンオイルに触れると破泡する。中空糸膜表面
のシリコングリス、又はシリコンオイルが消費されると
破泡効果は減少してしまうので、槽内で化学反応中。Silicone grease or silicone oil is injected into the hollow fiber membrane using a syringe through an injection port provided outside the tank. As a result of this injection, the injected solution evenly oozes out onto the surface of the hollow fibers M that are formed around the upper part of the liquid surface. Bubbles generated from the liquid in the tank are caused by silicone grease on the surface of the hollow fiber membrane,
Or if it comes into contact with silicone oil, the bubbles will break. If the silicone grease or silicone oil on the surface of the hollow fiber membrane is consumed, the bubble-breaking effect will decrease, so a chemical reaction is taking place in the tank.
又は培養中であっても槽外部に設けた注入口より再度溶
液を注入することによって再生することが可能となる。Alternatively, even during cultivation, it is possible to regenerate by injecting the solution again from the injection port provided outside the tank.
以下、本発明の一実施例を第１図により説明する。第１
図に示す楢は、微生物等の培養に用いる標準的な培養槽
２である。図において、培＃槽２での培養液は、エアー
供給口８より酸素又は空気が供給され、攪拌ｘ５で攪拌
・混合され、温調ジャケット３により温度がコントロー
ルされる。このように空気を供給しながら攪拌を行うと
培養液の粘性も手伝って泡が液面４より上部に生じる。An embodiment of the present invention will be described below with reference to FIG. 1st
The oak shown in the figure is a standard culture tank 2 used for culturing microorganisms and the like. In the figure, oxygen or air is supplied to the culture solution in the culture tank 2 from the air supply port 8, and the culture solution is stirred and mixed by the stirring x5, and the temperature is controlled by the temperature control jacket 3. When stirring is performed while supplying air in this manner, bubbles are generated above the liquid level 4 due to the viscosity of the culture solution.
そして培養槽２内に設けた破泡ネットｌまで泡が上昇し
、Ｈｆ＆泡ネマトｌに触れると、該破泡ネット１！！面
には、注入０７より注入したシリコングリス又はシリコ
ンオイルが一様に滲みでているため泡は破泡してしまう
。よって該破泡ネットｌより上部には泡が全く無く、培
養時、泡による悪影響は無くなる。Then, when the bubbles rise up to the foam-breaking net 1 provided in the culture tank 2 and touch the Hf & foam nematode 1, the foam-breaking net 1! ! Since the silicone grease or silicone oil injected from injection 07 is uniformly seeping out on the surface, the bubbles break. Therefore, there are no bubbles at all above the foam-broken net 1, and there is no adverse effect due to bubbles during culturing.
また動物ｍ胞培養においては、消泡剤を添加するとそれ
が増殖を阻害したりすることが多量あるので本発明のよ
うに破泡ネットを用いる効果が絶大である。Furthermore, in animal cell culture, when an antifoaming agent is added, it often inhibits proliferation, so the use of a foam-breaking net as in the present invention is extremely effective.
本−実施例の詳細をＮ２図に示す。図において、本体は
、テニスラケットのように、金属フレーム１１に穴をあ
け、数十本の中空糸セを編んだ構造である。これらの中
空糸セの両端を１か所に集め金属継手に接着している。Details of this example are shown in Diagram N2. In the figure, the main body has a structure similar to a tennis racket, with holes made in a metal frame 11 and dozens of hollow fibers woven together. Both ends of these hollow fibers are gathered in one place and adhered to a metal joint.
これら中空糸１２内には。Inside these hollow fibers 12.
金属継手の他方に取り付けているグリスニブプル１４か
ら、シリコングリス１５を注入している。長期間使用す
ると中空糸Ｕ内シリコングリス巧は消費されるので、シ
リコングリス１５の注入を金属継手のグリスニップル１
４より行うことが可能な構造をしている。本発明により
発泡する系における長期連続運転を可能とする絶大なる
効果がある。Silicone grease 15 is injected from a grease nib pull 14 attached to the other side of the metal joint. When used for a long period of time, the silicone grease inside the hollow fiber U will be consumed, so inject silicone grease 15 into the grease nipple 1 of the metal joint.
It has a structure that allows it to be carried out from 4 onwards. The present invention has the tremendous effect of enabling long-term continuous operation in a foaming system.
本実施例によれば、消泡剤の添加をすることなく破泡で
きるので、多量に用いる消泡剤をカットできる効果があ
る。According to this example, foam can be broken without adding an antifoaming agent, so there is an effect that the antifoaming agent used in a large amount can be cut.
また消泡剤を嫌う粘養系、化学反応系においては、消泡
剤にとって代る破泡ネットを用いれば、効果絶大となる
。Furthermore, in sticky systems and chemical reaction systems where antifoaming agents are not desirable, the use of a foam-breaking net instead of the antifoaming agent will be extremely effective.
本発明によれば、長期間連続的に使用される装置の槽内
破泡が容易にできる効果がある。According to the present invention, there is an effect that bubbles can be easily broken in a tank of an apparatus that is used continuously for a long period of time.
ｌ・・・・・・破泡ネット、２・・・・・・培養槽、４
・・・・・・液面、７・・・・・・注入口、８・・曲エ
アー供給０１１１・・・・・・フレーム、毘・・・・・
・中空糸、１４・・曲グリースニブプル、牙
２
目
第３Ｉ￥１l...Broam net, 2...Culture tank, 4
...Liquid level, 7...Inlet, 8...Curved air supply 0111...Frame, Bi...
・Hollow fiber, 14...Curved grease nib pull, fangs 2, 3rd I ￥1
Claims (1)
において、 前記破泡手段に疎水性の中空糸膜から成る格子状または
、平面状のネットを形成し、該ネットを槽内液面より上
部に設けると共に、該中空糸膜内には、槽外部より疎水
性溶液を注入可能に構成したことを特徴とする槽内破泡
装置。 ２、前記疎水性中空糸膜内にシリコン系油脂を注入する
ことを特徴とする請求項第１項記載の槽内破泡装置。[Claims] 1. An apparatus using a bubble breaking means for bubbles generated from a hydrophilic solution, wherein a lattice-like or planar net made of hydrophobic hollow fiber membranes is formed on the bubble breaking means, A bubble breaking device in a tank, characterized in that the net is provided above the liquid level in the tank, and a hydrophobic solution can be injected into the hollow fiber membrane from outside the tank. 2. The in-tank bubble breaking device according to claim 1, characterized in that a silicone oil or fat is injected into the hydrophobic hollow fiber membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20780589A JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20780589A JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0372864A true JPH0372864A (en) | 1991-03-28 |
| JP2810134B2 JP2810134B2 (en) | 1998-10-15 |
Family
ID=16545790
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20780589A Expired - Fee Related JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2810134B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006027012A (en) * | 2004-07-14 | 2006-02-02 | Pioneer Electronic Corp | Defoaming method and defoaming apparatus therefor |
| DE102018102703A1 (en) * | 2018-02-07 | 2019-08-08 | Technische Universität Dortmund | Reactor and process |
-
1989
- 1989-08-14 JP JP20780589A patent/JP2810134B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006027012A (en) * | 2004-07-14 | 2006-02-02 | Pioneer Electronic Corp | Defoaming method and defoaming apparatus therefor |
| DE102018102703A1 (en) * | 2018-02-07 | 2019-08-08 | Technische Universität Dortmund | Reactor and process |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2810134B2 (en) | 1998-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |