JP2810134B2 - In-tank foam breaker - Google Patents
In-tank foam breakerInfo
- Publication number
- JP2810134B2 JP2810134B2 JP20780589A JP20780589A JP2810134B2 JP 2810134 B2 JP2810134 B2 JP 2810134B2 JP 20780589 A JP20780589 A JP 20780589A JP 20780589 A JP20780589 A JP 20780589A JP 2810134 B2 JP2810134 B2 JP 2810134B2
- Authority
- JP
- Japan
- Prior art keywords
- tank
- hollow fiber
- fiber membrane
- culture
- foam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Degasification And Air Bubble Elimination (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、発泡を伴う槽内化学反応又は槽内培養にお
ける槽内破泡装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an in-tank foam breaker in an in-tank chemical reaction or in-tank culture involving foaming.
従来の消泡の方法としては、消泡剤を培養液中に投入
していたが、最近、培養槽中に消泡層を設けることを特
徴とした特開昭64〜67184号に記載の培養方法が考案さ
れた。上記公報では、長期にわたる連結培養（２か月以
上）について記載はなく、また該公報のような消泡層を
構成するものでは、長期的な培養については、破泡効果
が減少してくるものであった。それは培養液の初期に消
泡層を形成するSUS製の格子状の網状のものにシリコン
グリスを塗布したものであるため、培養時間の経過とと
もにシリコングリスが消費され、破泡能力が消滅してし
まうからである。As a conventional method of defoaming, an antifoaming agent has been introduced into a culture solution, but recently, a culturing method described in JP-A-64-67184 characterized by providing an antifoaming layer in a culture tank. A method was devised. In the above-mentioned publication, there is no description about long-term continuous culture (two months or more), and in the case of forming an antifoaming layer as in this publication, the long-term culture decreases the foam breaking effect. Met. Silicon grease is applied to the mesh-like mesh made of SUS, which forms an antifoam layer in the initial stage of the culture solution, so that silicon grease is consumed with the lapse of culture time, and the foam breaking ability disappears. It is because.
上記従来技術は、消泡ネット表面にシリコン系油脂が
塗布されているだけで、使用中に、塗布したシリコング
リス又は、シリコンオイルが消費され、数か月オーダー
の長期にわたり連続的に用いる場合には適していなかっ
た。The above-mentioned prior art is only applied with silicon-based fats and oils on the surface of the defoaming net.During use, the applied silicon grease or silicon oil is consumed, and when used continuously for a long period of several months. Was not suitable.
本発明の目的は、培養等比較的長期間にわたっての使
用される装置の槽内破泡装置を提供することにある。An object of the present invention is to provide an in-tank foam breaker for a device used for a relatively long period of time such as culturing.
上記目的を達成するために、疎水性の中空糸膜を用
い、該中空糸膜を３〜4mm間隔で液面の上部に格子状又
は平面状にはりめぐらせ、槽外部より該形成された中空
糸膜中にシリコンオイル又はシリコングリスを注入する
ように構成したものである。In order to achieve the above object, a hydrophobic hollow fiber membrane is used, and the hollow fiber membrane is wrapped around the upper surface of the liquid surface in a lattice or plane at intervals of 3 to 4 mm. It is configured such that silicone oil or silicone grease is injected into the yarn membrane.
槽外部に設けられた注入口よりシリコングリス、又は
シリコンオイルをシリンジを用いて中空糸膜内に注入す
る。該注入により液面上部に張りめぐって形成されてい
る中空糸膜表面に一様に注入した溶液が滲み出てくる。
槽内液より生じた泡は、中空糸膜表面のシリコングリ
ス、又はシリコンオイルに触れると破泡する。中空糸膜
表面のシリコングリス、又はシリコンオイルが消費され
ると破泡効果は減少してしまうので、槽内で化学反応
中、又は培養中であっても槽外部に設けた注入口より再
度溶液を注入することによって再生することが可能とな
る。Silicon grease or silicone oil is injected into the hollow fiber membrane using a syringe from an injection port provided outside the tank. Due to the injection, the uniformly injected solution oozes on the surface of the hollow fiber membrane stretched over the liquid surface.
Foam generated from the liquid in the tank breaks when it comes into contact with silicon grease or silicon oil on the surface of the hollow fiber membrane. When the silicon grease or silicon oil on the surface of the hollow fiber membrane is consumed, the foam breaking effect decreases, so even during a chemical reaction in the tank or during culture, the solution is re-entered from the inlet provided outside the tank. Can be regenerated by injecting.
以下、本発明の一実施例を第１図により説明する。第
１図に示す槽は、微生物等の培養に用いる標準的な培養
槽２である。図において、培養槽２での培養液は、エア
ー供給口８より酸素又は空気が供給され、撹拌翼５で撹
拌・混合され、温調ジャケット３により温度がコントロ
ールされる。このように空気を供給しながら撹拌を行う
と培養液の粘性も手伝って泡が液面４より上部に生じ
る。そして培養槽２内に設けた破泡ネット１まで泡が上
昇し、該破泡ネット１に触れると、該破泡ネット１表面
には、注入口７より注入したシリコングリス又はシリコ
ンオイルが一様に滲みでているため泡は破泡してしま
う。よって該破泡ネット１より上部には泡が全く無く、
培養時、泡による悪影響は無くなる。Hereinafter, an embodiment of the present invention will be described with reference to FIG. The tank shown in FIG. 1 is a standard culture tank 2 used for culturing microorganisms and the like. In the drawing, oxygen or air is supplied to a culture solution in a culture tank 2 from an air supply port 8, stirred and mixed by a stirring blade 5, and the temperature is controlled by a temperature control jacket 3. When the stirring is performed while supplying the air in this manner, bubbles are generated above the liquid level 4 due to the viscosity of the culture solution. When the foam rises to the foam breaking net 1 provided in the culture tank 2 and touches the foam breaking net 1, the surface of the foam breaking net 1 is uniformly filled with silicon grease or silicone oil injected from the injection port 7. The bubbles break because they are bleeding into the water. Therefore, there is no bubble above the breaking foam net 1,
During culturing, the adverse effects of bubbles are eliminated.
また動物細胞培養においては、消泡剤を添加するとそ
れが増殖を阻害したりすることが多々あるので本発明の
ように破泡ネットを用いる効果が絶大である。In addition, in animal cell culture, when an antifoaming agent is added, it often inhibits growth, and thus the effect of using a foam breaking net as in the present invention is enormous.
本一実施例の詳細を第２図に示す。図において、本体
は、テニスラケットのように、金属フレーム11に穴をあ
け、数十本の中空糸12を編んだ構造である。これらの中
空糸12の両端を１か所に集め金属継手に接着している。
これら中空糸12内には、金属継手の他方に取り付けてい
るグリスニップル14から、シリコングリス15を注入して
いる。長期間使用すると中空糸12内シリコングリス15は
消費されるので、シリコングリス15の注入を金属継手の
グリスニップル14より行うことが可能な構造をしてい
る。本発明により発泡する系における長期連続運転を可
能とする絶大なる効果がある。FIG. 2 shows the details of this embodiment. In the figure, the main body has a structure in which holes are made in a metal frame 11 and several tens of hollow fibers 12 are knitted like a tennis racket. Both ends of these hollow fibers 12 are gathered in one place and adhered to a metal joint.
Silicon grease 15 is injected into these hollow fibers 12 from a grease nipple 14 attached to the other of the metal joints. When used for a long time, the silicon grease 15 in the hollow fiber 12 is consumed, so that the silicon grease 15 can be injected from the grease nipple 14 of the metal joint. The present invention has a remarkable effect of enabling long-term continuous operation in a foaming system.
本実施例によれば、消泡剤の添加をすることなく破泡
できるので、多量に用いる消泡剤をカットできる効果が
ある。According to the present embodiment, since foam can be broken without adding an antifoaming agent, there is an effect that a large amount of antifoaming agent can be cut.
また消泡剤を嫌う粘養系，化学反応系においては、消
泡剤にとって代る破泡ネットを用いれば、効果絶大とな
る。In addition, in a viscous system or a chemical reaction system which dislikes an antifoaming agent, if a foaming net is used instead of the antifoaming agent, the effect is extremely large.
本発明によれば、長期間連続的に使用される装置の槽
内破泡が容易にできる効果がある。ADVANTAGE OF THE INVENTION According to this invention, there exists an effect which can easily break in the tank of the apparatus used continuously for a long period of time.
第１図は本発明の一実施例の槽内破泡装置の断面図、第
２図は本発明の破泡装置の詳細図、第３図は第２図のＩ
−Ｉ線断面図である。 １……破泡ネット、２……培養槽、４……液面、７……
注入口、８……エアー供給口、11……フレーム、12……
中空糸、14……グリースニップル、15……シリコングリ
ースFIG. 1 is a cross-sectional view of a foam breaking device in a tank according to one embodiment of the present invention, FIG. 2 is a detailed view of the foam breaking device of the present invention, and FIG.
FIG. 2 is a sectional view taken along line I. 1 ... foaming net, 2 ... culture tank, 4 ... liquid level, 7 ...
Inlet, 8 ... Air supply port, 11 ... Frame, 12 ...
Hollow fiber, 14 grease nipple, 15 silicon grease
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.6，ＤＢ名) C12M 1/00 - 3/10 B01D 19/02──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. 6 , DB name) C12M 1/00-3/10 B01D 19/02
Claims (2)
いた装置において、 前記破泡手段に疎水性の中空糸膜から成る格子状また
は、平面状のネットを形成し、該ネットを槽内液面より
上部に設けると共に、該中空糸膜内には、槽外部より疎
水性溶液を注入可能に構成したことを特徴とする槽内破
泡装置。An apparatus using means for breaking bubbles generated from a hydrophilic solution, wherein a lattice-shaped or planar net made of a hydrophobic hollow fiber membrane is formed in said means for breaking bubbles. An in-vessel bubble breaking device, wherein the inside of the hollow fiber membrane is provided so as to be able to inject a hydrophobic solution into the hollow fiber membrane from the outside of the tank.
注入することを特徴とする請求項第１項記載の槽内破泡
装置。2. The in-vessel foam breaking apparatus according to claim 1, wherein a silicone oil or fat is injected into said hydrophobic hollow fiber membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20780589A JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20780589A JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0372864A JPH0372864A (en) | 1991-03-28 |
| JP2810134B2 true JP2810134B2 (en) | 1998-10-15 |
Family
ID=16545790
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20780589A Expired - Fee Related JP2810134B2 (en) | 1989-08-14 | 1989-08-14 | In-tank foam breaker |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2810134B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006027012A (en) * | 2004-07-14 | 2006-02-02 | Pioneer Electronic Corp | Defoaming method and defoaming apparatus therefor |
| DE102018102703A1 (en) * | 2018-02-07 | 2019-08-08 | Technische Universität Dortmund | Reactor and process |
-
1989
- 1989-08-14 JP JP20780589A patent/JP2810134B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0372864A (en) | 1991-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4894342A (en) | Bioreactor system | |
| US4918019A (en) | Bioreactor system with plasticizer removal | |
| US7892821B2 (en) | Culture chamber, culture apparatus and liquid supplying method for cell or tissue cultivation | |
| ES545836A0 (en) | PROCEDURE AND APPARATUS FOR THE EXEMPTED GASPING OF LIQUID BUBBLES, ESPECIALLY FOR CULTIVATION MEANS PARTICULARLY INTENDED TO PROPAGATE TISSUE CROPS | |
| JP2810134B2 (en) | In-tank foam breaker | |
| JP4475280B2 (en) | Cell culture method and cell culture apparatus | |
| BR9507330A (en) | Rectangular flotation device | |
| KR101139702B1 (en) | An apparatus for cultivating aerotropic/anaerobic microorganism in a body | |
| SE7910260L (en) | SET AND APPARATUS FOR CLEANING OF WETS FROM CHAMBERS, WHICH REPRESENT ARE MADE UP | |
| ATE133146T1 (en) | METHOD AND APPARATUS FOR THE PROPAGATION OF SELECTED STRAINS USED FOR THE TREATMENT OF FATS AND STARCHES | |
| JPH09503643A (en) | Foam prevention device for bioreactor | |
| JP2007202542A (en) | Cell culture vessel | |
| JP4637329B2 (en) | Hydrogen dissolver | |
| Semmens | Bubbleless aeration | |
| JPH09140282A (en) | Apparatus for hydroponics | |
| JP2751463B2 (en) | Method for manufacturing hollow fiber membrane module | |
| SU901266A1 (en) | Chamber for culturing microorganisms | |
| CN215505628U (en) | Defoaming device and circulating system utilizing reclaimed water | |
| JPH03172170A (en) | Method for cell culture and system therefor | |
| CN212881168U (en) | Salt chemical wastewater treatment foam alarm device | |
| JPH02249478A (en) | Bioreactor for alcohol fermentation | |
| JPS62236430A (en) | Hydroponic apparatus | |
| CN217245851U (en) | Pancreas perfusion device capable of automatically controlling pressure | |
| JPH0229293B2 (en) | ||
| JPH03160983A (en) | Culturing device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |