JPH0325598Y2 - - Google Patents

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Publication number
JPH0325598Y2
JPH0325598Y2 JP4481584U JP4481584U JPH0325598Y2 JP H0325598 Y2 JPH0325598 Y2 JP H0325598Y2 JP 4481584 U JP4481584 U JP 4481584U JP 4481584 U JP4481584 U JP 4481584U JP H0325598 Y2 JPH0325598 Y2 JP H0325598Y2
Authority
JP
Japan
Prior art keywords
culture
heat
stage
insulating box
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP4481584U
Other languages
Japanese (ja)
Other versions
JPS60156996U (en
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Priority to JP4481584U priority Critical patent/JPS60156996U/en
Priority to US06/671,732 priority patent/US4629862A/en
Priority to DE19853511165 priority patent/DE3511165A1/en
Publication of JPS60156996U publication Critical patent/JPS60156996U/en
Application granted granted Critical
Publication of JPH0325598Y2 publication Critical patent/JPH0325598Y2/ja
Granted legal-status Critical Current

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Description

【考案の詳細な説明】 技術分野 本考案は、顕微鏡の載物台に固定的に配設して
培養標本を適正な保温状態において顕微鏡により
観察し得るようにした顕微鏡観察のための培養装
置に関する。
[Detailed Description of the Invention] Technical Field The present invention relates to a culture device for microscopic observation, which is fixedly disposed on the stage of the microscope so that the cultured specimen can be observed under the microscope while kept at an appropriate temperature. .

従来技術 従来使用されている顕微鏡観察のための培養装
置においては、培養観察容器内の培養標本の保温
は、フアンを使用して保温箱内に温風を吹込み培
養観察容器及びその周囲を加温することにより、
行なわれていた。しかしながら、この方式は、以
下の欠点即ち、 (1) 室内の空気をヒーターで加熱した後フアンを
使用して保温箱内に温風を吹込むようになつて
いるために、観察すべき培養標本に有害な埃が
つく危険性がある; (2) 保温箱内に温風を吹込む際、空気の熱容量が
小さいために培養標本が所定温度になるまでの
応答時間が長く、特に培養標本の温度を時間的
にプログラム制御する場合に非常に時間がかか
り十分な性能が得られない; (3) フアンを使用して保温箱内に温風を吹込むた
めに、保温箱内の埃が舞い上がり培養標本につ
く危険性がある; (4) 開放形の培養観察容器を使用する場合、培養
観察容器内の培養液から蒸発した水蒸気が保温
箱の内面に付着して曇りを生じるため、観察が
妨げられる; という欠点を有している。
Prior Art In conventional culture apparatuses for microscopic observation, the culture specimens in the culture observation container are kept warm by blowing warm air into the heat insulation box using a fan to heat the culture observation container and its surroundings. By heating,
It was being done. However, this method has the following drawbacks: (1) Since the indoor air is heated with a heater and then a fan is used to blow warm air into the insulated box, it may be harmful to the culture specimens to be observed. (2) When blowing hot air into the heating box, the response time for the culture specimen to reach the specified temperature is long because the heat capacity of the air is small. When using time-based program control, it takes a very long time and sufficient performance cannot be obtained; (3) Since a fan is used to blow warm air into the insulating box, dust inside the insulating box flies up and sticks to the culture specimens. (4) When using an open culture observation container, water vapor evaporated from the culture solution in the culture observation container will adhere to the inner surface of the insulated box and cause cloudiness, which will hinder observation; It has drawbacks.

目 的 本考案は、以上の点に鑑み、フアンを介して保
温箱内に埃が導入されず、また保温箱内の埃が舞
い上らず、培養標本の所定温度への温度制御の応
答時間が短く、而も開放形の培養観察容器を使用
する場合にも保温箱の内面に曇りが生じないよう
にした顕微鏡観察のための培養装置を提供するこ
とを目的としている。
Purpose In view of the above points, this invention prevents dust from being introduced into the insulating box through the fan, prevents dust from flying up inside the insulating box, and reduces the response time of temperature control to a predetermined temperature of the culture specimen. To provide a culture device for microscopic observation which has a short length and which prevents clouding on the inner surface of a heat-insulating box even when an open culture observation container is used.

概 要 この目的は、少なくとも一部が発熱部として構
成されている顕微鏡の載物台と、該載物台の発熱
部上面に載置された培養観察容器を包囲する第一
の空間を画成している第一の保温箱と、該第一の
保温箱を包囲する第二の空間を該第一の保温箱と
共に画成している第二の保温箱と、該第二の空間
を加熱するための加熱手段とを備えていることを
特徴とする顕微鏡観察のための培養装置により解
決される。
Overview This purpose is to define a first space that surrounds a microscope stage, at least a part of which is configured as a heat generating part, and a culture observation container placed on the top surface of the heat generating part of the stage. a first heat insulating box that is heated, a second heat insulating box defining a second space surrounding the first heat insulating box together with the first heat insulating box, and heating the second space; The present invention is solved by a culture device for microscopic observation, characterized in that it is equipped with a heating means for

さらに、好ましくは本装置は、載物台の発熱部
の温度を検出して該温度と設定温度とを比較する
ことにより前記発熱部の温度をフイードバツク制
御するための温度制御装置を含んでいる。
Further, preferably, the present apparatus includes a temperature control device for feedback controlling the temperature of the heat generating part by detecting the temperature of the heat generating part of the stage and comparing the detected temperature with a set temperature.

実施例 以下図面に示した実施例により本考案を説明す
れば、第1図は倒立型顕微鏡に本考案を適用した
場合の実施例であり、1は倒立型顕微鏡、2は本
考案による培養装置である。この培養装置2はさ
らに第2図及び第3図にて詳細に示されており、
3は顕微鏡1の載物台で、発熱部として構成され
た部分3aと、該部分3aが着脱可能に取付けら
れ且つ紙面に対して垂直方向に移動し得る部分3
bとを有している。4は載物台部分3aの上面に
載置された培養観察容器5を包囲する第一の空間
6を画成している気密性の第一の保温箱で、載物
台部分3bの上面にネジ7により固定的に配設さ
れた周壁4aと蓋4bとから構成されていて、該
周壁4bは例えば観察すべき培養標本にCO2等の
ガスが必要である場合にガスの注入を行なうため
のチユーブ8を図示の如く取付けるための取付口
を備えており、蓋4bは、顕微鏡1のコンデンサ
レンズ9の鏡筒先端部が嵌入し得ると共に下端が
透明部材10により閉塞されている孔4cと、試
薬注入または温度センサー、湿度センサー等の挿
入のための各々蓋11により開閉可能になつてい
る複数の(図面では四つの)孔4dと、培養観察
容器5を包囲するように該蓋4bの下面で前記孔
4dより外側に設けられたフランジ部4eとを有
しており、周壁4aの上縁に対して気密的に摺動
し得るように構成されている。12は載物台部分
3aの発熱部に給電するためのコードでコネクタ
13を介して温度制御装置14に接続されてお
り、15は載物台部分3aの上面に配設された温
度センサーで、そのコードは同様に温度制御装置
14に接続されている。16は第一の保温箱4を
包囲する第二の空間17を該第一の保温箱4と共
に画成している気密性の第二の保温箱で、第一の
保温箱4の外縁部に締具18等により固定するこ
とにより載物台部分3bの上面に対して固定的に
配設された周壁16aと、該周壁16aに対して
固定されていて且つ中央に顕微鏡1のコンデンサ
レンズ9の鏡筒径に比較して十分に大きい孔を有
する蓋16bと、該蓋16b上に摺動可能に載置
され且つ中央に該コンデンサレンズ9が嵌合し得
る孔を有する摺動部材16cとから構成されてお
り、周壁16aは第二の空間17内に温風を吹込
むために温風フアン19に接続されたチユーブ2
0を図示の如く取付けるための取付口21を備え
ている。尚、22は倒立型顕微鏡1の対物レン
ズ、23は環状の水容器である。
EXAMPLES The present invention will be explained below using examples shown in the drawings. Figure 1 shows an example in which the present invention is applied to an inverted microscope, 1 is an inverted microscope, and 2 is a culture device according to the present invention. It is. This culture device 2 is further shown in detail in FIGS. 2 and 3,
Reference numeral 3 denotes a stage for the microscope 1, which includes a portion 3a configured as a heat generating portion, and a portion 3 to which the portion 3a is removably attached and movable in a direction perpendicular to the plane of the paper.
It has b. Reference numeral 4 designates an airtight first insulating box defining a first space 6 surrounding the culture observation container 5 placed on the top surface of the stage portion 3a; It is composed of a peripheral wall 4a and a lid 4b which are fixedly arranged by screws 7, and the peripheral wall 4b is used for injecting gas such as CO 2 when a culture specimen to be observed requires gas, for example. The lid 4b has a hole 4c into which the tip of the lens barrel of the condenser lens 9 of the microscope 1 can fit, and whose lower end is closed by a transparent member 10. , a plurality of holes 4d (four in the drawing) each of which can be opened and closed by a lid 11 for injecting a reagent or inserting a temperature sensor, a humidity sensor, etc., and a hole 4d in the lid 4b so as to surround the culture observation container 5. It has a flange portion 4e provided on the lower surface outside the hole 4d, and is configured to slide airtightly against the upper edge of the peripheral wall 4a. 12 is a cord for supplying power to the heat generating part of the stage part 3a, and is connected to the temperature control device 14 via the connector 13; 15 is a temperature sensor disposed on the upper surface of the stage part 3a; The cord is likewise connected to temperature control device 14. Reference numeral 16 denotes an airtight second heat insulation box that defines a second space 17 surrounding the first heat insulation box 4. A peripheral wall 16a is fixedly disposed on the upper surface of the stage portion 3b by fixing with a fastener 18, etc., and a condenser lens 9 of the microscope 1 is fixed to the peripheral wall 16a and located in the center. A lid 16b having a hole that is sufficiently large compared to the diameter of the lens barrel, and a sliding member 16c that is slidably placed on the lid 16b and has a hole in the center into which the condenser lens 9 can fit. The surrounding wall 16a has a tube 2 connected to a hot air fan 19 to blow hot air into the second space 17.
0 as shown in the figure. Note that 22 is an objective lens of the inverted microscope 1, and 23 is an annular water container.

本案実施例は以上のように構成されているか
ら、先づ観察すべき培養標本を収容した培養観察
容器5を顕微鏡1の載物台3の発熱部として構成
されている部分3a上に載置し、湿度を必要とす
る培養標本の場合には水容器23に水を入れて同
様に載物台部分3a上に載置する。次に温度制御
装置14により設定温度を入力して載物台部分3
aに給電し、該部分3aの発熱により培養標本を
収容した培養観察容器5を直接的に加熱し、また
自然対流の熱伝達により第一の保温箱6内の空気
が加温され、かくして培養標本の保温が行なわれ
る。このとき、第二の空間17内には温風フアン
19からチユーブ20を介して温風が吹込まれ、
これにより該第二の空間17が加温され、かくし
て第一の保温箱4を、その内側表面温度が第一の
空間6の空気温度より高くなるまで、加温するこ
とによつて、培養標本または水容器23内の水か
ら蒸発した水蒸気が第一の保温箱4の内側表面に
凝縮しない。尚、第一の保温箱4の蓋4bは、コ
ンデンサレンズ9がその孔4cに嵌合しているの
で、載物台3を移動した場合にはこれと共に移動
する該第一の保温箱4の周壁4aに対して摺動す
ることにより、その位置は変らない。従つて該蓋
4bに設けられた孔4dは図示の如く常に光軸上
の焦点位置に向いており、載物台3を移動しても
常に観察位置に向つて試薬の注入等を行なうこと
ができるが、該孔4dは必要に応じて他の方向に
向けて形成されていてもよい。また、培養標本の
観察範囲は、載物台3を第4図の如く限界位置ま
で移動させると載物台3と共に移動する培養観察
容器5が途中で第一の保温箱4の蓋4bの下面に
設けられたフランジ部4eにより係止されるため
載物台3に対して距離lだけ移動するので、載物
台3の部分3aの中座孔径をAとすればφ{A+
2l}となる。
Since the present embodiment is constructed as described above, the culture observation container 5 containing the culture specimen to be observed is first placed on the portion 3a of the stage 3 of the microscope 1, which is configured as a heat generating part. However, in the case of a culture specimen that requires humidity, the water container 23 is filled with water and placed on the stage portion 3a in the same manner. Next, input the set temperature using the temperature control device 14 and set the temperature on the stage 3.
a, the heat generated by the section 3a directly heats the culture observation container 5 containing the culture specimen, and the air in the first warming box 6 is heated by the heat transfer of natural convection, thus allowing the culture to proceed. The specimen is kept warm. At this time, warm air is blown into the second space 17 from the hot air fan 19 via the tube 20,
As a result, the second space 17 is heated, and by heating the first insulating box 4 until its inner surface temperature becomes higher than the air temperature in the first space 6, the culture specimen is heated. Alternatively, the water vapor evaporated from the water in the water container 23 does not condense on the inner surface of the first heat insulation box 4. In addition, since the condenser lens 9 is fitted into the hole 4c of the lid 4b of the first heat-insulating box 4, when the stage 3 is moved, the lid 4b of the first heat-insulating box 4 that moves together with the stage 3 is moved. By sliding against the peripheral wall 4a, its position does not change. Therefore, the hole 4d provided in the lid 4b always faces the focal point on the optical axis as shown in the figure, and even if the stage 3 is moved, reagents can always be injected toward the observation position. However, the holes 4d may be formed in other directions as necessary. In addition, the observation range of the culture specimen is such that when the stage 3 is moved to the limit position as shown in FIG. Since it is locked by the flange portion 4e provided on the stage 3, it moves by a distance l relative to the stage 3. Therefore, if the diameter of the center hole in the part 3a of the stage 3 is A, then φ{A+
2l}.

第5図は本考案の第二の実施例を示しており、
第2図の実施例と同一の部材には同じ符号を付し
てその説明を省略すれば、30は顕微鏡1の載物
台3の本体下側にネジにより固定された第二の保
温箱で、その蓋30aは周壁30bに対して固定
されていて且つ中央に顕微鏡1のコンデンサレン
ズ9が嵌合し得る孔を有しており、第一の保温箱
4と第二の保温箱30との間に画成された第二の
空間31内にはヒーター32が配設されており、
該第二の空間31はヒーター32に通電されるこ
とにより第一の実施例における第二の空間17と
同様に加温される。
FIG. 5 shows a second embodiment of the present invention,
Components that are the same as those in the embodiment shown in FIG. The lid 30a is fixed to the peripheral wall 30b and has a hole in the center into which the condenser lens 9 of the microscope 1 can fit, and the lid 30a is fixed to the peripheral wall 30b, and has a hole in the center into which the condenser lens 9 of the microscope 1 can be fitted. A heater 32 is disposed within a second space 31 defined between the two.
The second space 31 is heated in the same way as the second space 17 in the first embodiment by energizing the heater 32.

考案の効果 以上述べたように本考案によれば、培養標本は
載物台により直接的に加温されるために応答時間
が短く、培養標本を包囲する空間が密閉されてい
るために有害な埃が培養標本の周囲に持ち込まれ
ず且つ保温箱内の埃が舞い上がらず従つて培養標
本に有害な埃がつくことがなく、第一の保温箱の
内側表面が曇るようなことがなく保温箱が載物台
上面にあるのでレボルバーの変換や対物レンズの
補正環の操作等を妨げることがない。さらに、観
察位置を移動しても常に観察位置に向つて試薬の
注入等を行なうことができ、また培養標本と載物
台の相対位置を変えることにより広い観察範囲が
得られるために、保温箱の蓋を開けずに試薬の注
入や培養標本の位置調整が行なわれるので、保温
箱内のCO2濃度や温度制御された空気が逃げたり
埃が入り込んだりということが起こらない。さら
に第二の実施例の場合には、載物台を移動させて
も保温箱は移動しないのでヒーターのコードが移
動により引張られるようなことがなく観察視野が
不用意に動いてしまうようなことがなく、第二の
空間がヒーターにより加温されるため場合によつ
ては培養標本に対して有害であるような振動が生
じることはない。
Effects of the invention As described above, according to the invention, the response time is short because the cultured specimen is directly heated by the mounting stage, and the space surrounding the cultured specimen is sealed, causing harmful Dust is not carried around the cultured specimens, dust inside the insulated box is not stirred up, and therefore no harmful dust is attached to the cultured specimens, and the inner surface of the first insulated box is not clouded. Since it is located on the top surface of the stage, it does not interfere with the conversion of the revolver or the operation of the correction ring of the objective lens. Furthermore, even if the observation position is moved, reagents can always be injected toward the observation position, and a wide observation range can be obtained by changing the relative position of the cultured specimen and the stage. Since reagents are injected and culture specimens are adjusted without opening the lid, the CO 2 concentration and temperature-controlled air inside the insulated box will not escape, and dust will not enter. Furthermore, in the case of the second embodiment, even if the stage is moved, the insulating box does not move, so the cord of the heater will not be pulled due to movement, and the observation field of view will not be moved inadvertently. Since the second space is heated by the heater, vibrations that may be harmful to the culture specimen do not occur.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は本考案を適用した倒立型顕微鏡の概略
側面図、第2図は本考案による培養装置の第一の
実施例を示す断面図、第3図は第2図の実施例の
−線断面図、第4図は載物台を限界位置まで
移動させた状態を示す第2図と同様の図、第5図
は本考案の第二の実施例を示す図である。 1……倒立型顕微鏡、2……培養装置、3……
載物台、4……第一の保温箱、5……培養観察容
器、6……第一の空間、7……ネジ、8,20…
…チユーブ、9……コンデンサレンズ、10……
透明部材、11……蓋、12……コード、13…
…コネクタ、14……温度制御装置、15……温
度センサー、16……第二の保温箱、17……第
二の空間、18……締具、19……温風フアン、
21……取付口、22……対物レンズ、23……
水容器。
Fig. 1 is a schematic side view of an inverted microscope to which the present invention is applied, Fig. 2 is a sectional view showing a first embodiment of the culture device according to the present invention, and Fig. 3 is a line taken along the - line of the embodiment of Fig. 2. A cross-sectional view, FIG. 4 is a view similar to FIG. 2 showing a state in which the stage has been moved to the limit position, and FIG. 5 is a view showing a second embodiment of the present invention. 1...Inverted microscope, 2...Culture device, 3...
Loading stand, 4... First heat insulation box, 5... Culture observation container, 6... First space, 7... Screw, 8, 20...
...Tube, 9...Condenser lens, 10...
Transparent member, 11...lid, 12...cord, 13...
... Connector, 14 ... Temperature control device, 15 ... Temperature sensor, 16 ... Second heat insulation box, 17 ... Second space, 18 ... Fastener, 19 ... Warm air fan,
21...Mounting port, 22...Objective lens, 23...
water container.

Claims (1)

【実用新案登録請求の範囲】 (1) 顕微鏡本体と、少なくとも一部が発熱部とし
て構成され且つ移動自在に構成されている顕微
鏡の載物台と、該載物台の発熱部上面に載置さ
れた培養観察容器を包囲する第一の空間を画成
している第一の保温箱と、該第一の保温箱を包
囲する第二の空間を該第一の保温箱と共に画成
している第二の保温箱と、前記第二の空間を加
熱するための加熱手段とを備えていることを特
徴とする、顕微鏡観察のための培養装置。 (2) 上記第二の保温箱は顕微鏡本体に着脱自在に
固定したことを特徴とする、実用新案登録請求
の範囲第(1)に記載の顕微鏡観察のための培養装
置。
[Scope of Claim for Utility Model Registration] (1) A microscope main body, a movable microscope stage whose at least a portion is configured as a heat generating part, and a microscope mounted on the top surface of the heat generating part of the stage. a first heat-insulating box defining a first space surrounding the culture observation container; and a second space surrounding the first heat-insulating box defined together with the first heat-insulating box. 1. A culture device for microscopic observation, comprising: a second heat-insulating box; and heating means for heating the second space. (2) The culture device for microscopic observation according to claim 1 of the utility model registration, characterized in that the second heat-insulating box is detachably fixed to the microscope body.
JP4481584U 1984-03-28 1984-03-28 Culture device for microscopic observation Granted JPS60156996U (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP4481584U JPS60156996U (en) 1984-03-28 1984-03-28 Culture device for microscopic observation
US06/671,732 US4629862A (en) 1984-03-28 1984-11-15 Sample heater for use in microscopes
DE19853511165 DE3511165A1 (en) 1984-03-28 1985-03-27 SAMPLE HEATING DEVICE FOR MICROSCOPE

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4481584U JPS60156996U (en) 1984-03-28 1984-03-28 Culture device for microscopic observation

Publications (2)

Publication Number Publication Date
JPS60156996U JPS60156996U (en) 1985-10-18
JPH0325598Y2 true JPH0325598Y2 (en) 1991-06-03

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Application Number Title Priority Date Filing Date
JP4481584U Granted JPS60156996U (en) 1984-03-28 1984-03-28 Culture device for microscopic observation

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US7799559B2 (en) 2003-10-24 2010-09-21 Olympus Corporation Culture microscope apparatus
JP2006003653A (en) * 2004-06-17 2006-01-05 Olympus Corp Biological sample observating system
JP4354446B2 (en) * 2005-10-13 2009-10-28 株式会社東海ヒット Microscope stage and microscope observation unit
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US6980293B1 (en) 2002-06-11 2005-12-27 Olympus Optical Co., Ltd. Immersion medium supply apparatus, fluorescence spectrometry inspection apparatus, and culture microscope
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JP2008261940A (en) * 2007-04-10 2008-10-30 Olympus Corp Microscope apparatus

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