JPH03200A - Treatment of waste liquid after production of shochu by distillation - Google Patents

Abstract

PURPOSE:To remarkably improve the filterability of waste liq. after the production of SHOCHU (low-class distilled spirit) by distillation by producing polysaccharide-degrading multi-enzyme complex belonging to Streptomyces genus and adding the enzyme to the waste liq. CONSTITUTION:When waste liq. discharged from a distilling stage for producing SHOCHU is treated, cultured and/or treated bacteria producing polysaccharide- degrading enzyme belonging to Streptomyces genus are added to the waste liq. and this liq. is heated and held under stirring for a certain time. An enzyme reaction proceeds, polysaccharides are especially degraded and the physical properties of insoluble solid matter, that is, cell wall-forming polysaccharides in the waste liq. are changed, so the filterability of the waste liq. can be remarkably improved.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a method for treating waste liquid, and more specifically, a method for disposing of distillation waste liquid discharged from a shochu distillation process. The present invention relates to a method for improving the filtration performance of shochu distillation effluent in order to improve the violet filtration performance of distillation effluent, which is the technical obstacle mentioned above.

Therefore, the present invention is useful not only in the brewing industry but also in the pollution prevention industry such as wastewater treatment, and since the solid content separated by filtration can be effectively used as feed fertilizer, the present invention is applicable to the fertilizer industry and the paulownia material industry. It is also used heavily in industry.

(Prior art and problems) Distillation is an indispensable and important step in the shochu manufacturing process, but no appropriate method has been found to dispose of the distillation residue discharged during this process. The biggest problem is the poor filterability of this distillation waste liquid. For example, even when distillation waste liquid is treated by an activated sludge treatment method, efficient active pollution treatment is possible if solids with a high BOD loading rate are removed in advance.

At present, such a disposal method is not possible due to the difficulty of filtering the distillation waste liquid itself. In addition, some shochu manufacturers are attempting to separate solid-liquid distillation waste using a so-called compressor, but this method has problems such as the guinea wood becoming clogged quickly. are unsuitable for industrial processing, and face practical difficulties. Therefore, currently we have no choice but to dispose of shochu distillation waste by dumping it into the ocean, etc. However, it is clear that such a method of disposal is not a preferable method in terms of environmental conservation, and laws and regulations There are also moves to ban it. Therefore, there is an urgent need to establish an appropriate method for improving the filterability of shochu distillation waste liquid.

(Means for Solving the Problems) The present invention was made to meet the needs of this industry, and as a result of examination from various aspects, it was found that the main cause of the filterability of shochu distillation waste liquid is: It was discovered that the filter media was clogged by insoluble solids in the wastewater, especially cell wall polysaccharides.

Therefore, there is a method to prevent or reduce clogging of filter media caused by cell wall polysaccharides, and it is not only highly efficient, but also
In developing a low-cost industrial method that does not cause pollution and can be carried out under mild conditions, the present inventors focused on microorganisms.

It is also involved in the decomposition of these cell wall polysaccharides.

When searching for bacteria that produce an enzyme that improves the filterability of shochu distillation effluent, an actinomycete strain (strain 20-3-A) belonging to the genus Streptomyces was isolated from soil in Nagasaki Prefecture.

Therefore, when this strain was cultured and the produced polysaccharide decomposition complex enzyme was applied directly to the shochu distillation waste water, the enzyme activity was not inhibited by the distillation waste water and the filterability of the shochu distillation waste water was improved. It turns out that it can be significantly improved.

As a result of further research based on this useful new knowledge 1
The present inventors discovered for the first time that the actinomycete strain 2O-3-A, which belongs to Streptomyces and was newly isolated from the soil near Nagasaki Ito, is effective in treating shochu waste liquid.

The characteristics of the above-mentioned actinomycete strain 2O-3-A in terms of morphology, culture, and physiology are as follows.

1. Form 2O-3-A strain is a commonly used agar medium,
It has septa and forms branching aerial hyphae and basal hyphae. Note that no characteristic division of basal hyphae was observed, and no formation of sporangia or sclerotia was found.

Spores are formed in long chains of 10 or more on sporophores that are simply branched from aerial hyphae, and their morphology is straight or slightly curved. The size of mature spores is 0.5-0.7 μs
It is 1.0 to 1.4 μm, has an oval or columnar shape, and has a smooth surface without flagella.

(2) Growth status on various media The 2O-3-A strain shows normal or vigorous growth on commonly used synthetic and natural media, and forms gray aerial hyphae. On the other hand, basal hyphae generally exhibit a pale yellow color.
Very little soluble pigment is produced.

The characteristics of growth and color when cultured on various media at 28°C for 2 weeks are shown below. In addition, the color display is C, color
Harmony Manual (Container
The color classification according to the Corporation of America was followed.

1. Growth on glucose-asparagine agar medium, back color: normal, light eye poly-(2ca) aerial mycelium: normal,
White (a) - Natural (3dc) Soluble pigment: None 2, Glycerol/Asparagine agar - Growth, back color: Good, Light feet (2aa) Aerial mycelium: Abundant, Ash (5fa) Soluble pigment: None 3, Growth on sucrose/nitrate agar medium, back color: normal, light eye poly-(2ca) aerial hyphae: poor, white (a), soluble pigment: none 4, growth on starch/inorganic salt agar medium, back color: good , camel (3ie) aerial mycelium:
Abundant, white (a) - ash (5fe) Soluble pigment: none 5, grown on tyrosine agar medium, back color: good, light mustard tan (2ie
) Aerial hyphae abundant, natural (3dc) soluble pigment nibrown 6, grown on nutrient agar medium, back color: normal, light foot (2ea) aerial hyphae: poor, white (a) soluble pigment: none 7, malt extract・Yeast extract agar medium growth, back color: good, light feet (2ea) aerial hyphae: abundant, white (a) soluble pigment: none 8, oatmeal agar medium growth, back color: good, light eye poly( 2ca) Aerial mycelium: Abundant, white (a) - ash (5fs) Soluble pigment: None 9, Peptone/yeast/iron agar medium growth, Underside color: Normal, light beige (3ec) Aerial mycelium: None Soluble pigment Physiological Properties The physiological properties of the 2O-3-A strain are shown below. The temperature is 6
For other cases, the results are described after 2 weeks.

(1) Utilization of carbon source - Pridham-Gottlieb inorganic medium (Engineering SP No. 9) was used as a basic medium with 0.0% yeast extract.
The one containing 1% was used.

The 2O-3-A strain assimilates glucose, D-xylose, mannitol, fructose, L-rhamnose, L-arabinose, j-inositol, and sucrose, but does not assimilate raffinose.

(2) Liquefaction of gelatin: None 3) Effect on milk: Coagulation and liquefaction (4) Hydrolysis of starch: Yes (5) Growth temperature range: 21°C to 34.5°C (6) Production of tyrosinase : Yes (7) Production of melanoid pigment: Yes ■, Cell wall composition Analysis of diaminopimelic acid by whole bacterial cell hydrolysis:
Only LL-diaminopimelic acid was detected.

Based on the spore chains formed on the aerial hyphae and the steric type of diaminopimelic acid, this strain is classified as a member of the genus Streptomyces among actinomycetes.

In order to identify the species of this strain in the genus Streptomyces, the characteristics of this strain are: gray aerial hyphae, ■ straight spore chains, ■ smooth spore surface, ■ production of melanin-like pigment, and ■ soluble pigment. In consideration of the fact that no carbon is produced, ■ the availability of carbon sources, etc., an approved list of bacterial scientific names (Int, J, 5yst,
Bacteriol, 30: 225°1980
)) and also Int, J, 5yst.

As a result of searching among known bacterial strains published in the journal Bactariol, Streptomyces antebiotica was found to be the most closely related bacterial strain.
esantibioticus) and Streptomyces bushoud venezuelae (Streptomyca
speudovanezuelaa). However, William (S, T, Williams)
Numerical classification of et al. (J, Gen.

Microbiol, 129: 1743-181
3.1983) reported that these two species belong to different groups.

Therefore, this strain was used as Streptomyces sp.
3-A (Streptomyces sp, 2O-
It was named 3-A) and deposited with the National Institute of Microbial Technology, Agency of Industrial Science and Technology as Microbiological Laboratories No. 10690.

To carry out the method of the present invention, the polysaccharide decomposition complex enzyme related to the present strain may be isolated or used without isolation, and the enzymatic reaction may be carried out under suitable conditions6. A culture containing a polysaccharide decomposition complex enzyme obtained by culturing and/or a processed product thereof, such as a culture liquid supernatant, is added to the shochu distillation waste liquid as it is or after being concentrated or purified as necessary, - By carrying out the enzymatic reaction under certain conditions, the filterability of the shochu distillation effluent is significantly improved.

For industrial treatment, it is sufficient to add a culture of this strain and/or its treated product to the wastewater, adjust the pH if necessary, and then maintain the temperature while stirring for a certain period of time. The desired enzymatic reaction progresses, particularly polysaccharides are decomposed, the physical properties of the waste liquid are improved, and the filterability is greatly improved.

In the present invention, the culture refers to the cells of the present strain, cell extract, culture solution, culture solution filtrate, supernatant, and mixtures thereof, and the processed product refers to the concentrate of the above-mentioned culture. , pastes, dried products, diluted products, as well as purified and/or crude polysaccharide decomposition complex enzymes themselves, their contents, their concentrates, their diluted products, etc.

In other words, the culture and/or its processed product in the present invention broadly includes not only the polysaccharide decomposition complex enzyme itself related to the five bacterial strains, but also those containing the enzyme and those that produce the enzyme. be. In addition, when culturing this strain, using a medium containing the wastewater to be treated and/or pectin and other plant cell wall polysaccharides will promote the production of polysaccharide degrading complex enzymes by this strain. .

By performing such an enzyme treatment, the filtration treatment of the distillation waste liquid becomes easy, so that solids with a high BOD loading rate can be easily removed in advance. As a result, the filtrate can be easily separated without clogging the filter medium, so that any known wastewater treatment can be used freely as long as the filtrate is left alone. Therefore, for example, this filtrate can be sufficiently treated by the activated sludge treatment method, and as a result, the waste liquid can be treated very efficiently as a whole. On the other hand, according to this method, solid-liquid separation can be performed successfully without using harmful additives, so the solid content can be recovered and reused as a useful resource. The effects are significant.

As mentioned above, the present invention can be used very effectively for the treatment of shochu distillation effluent; however, the present invention can be used only for the treatment of shochu distillation effluent such as barley, rice, potatoes, barnyard millet, millet, carrots, etc. In addition, it can also be advantageously used as distillation effluent from other alcoholic beverages. Other distillation effluents, such as brandy, whiskey, carbatos, rum, awamori, and various other distillation effluents, can be widely used, but in that case, the strain should be cultured in a medium to which the distillation effluent is added. It is preferable.

The present invention will be described in more detail with reference to Examples 6. Example 1 Streptomyces sp.
After shaking culture for 1 day, centrifugation was performed and the supernatant was collected. This supernatant was concentrated 30 times using an ultrafiltration membrane with a molecular weight cutoff of 10,000 to obtain a concentrated crude enzyme solution containing polysaccharide decomposition enzymes. These 20 concentrated crude enzyme solutions were added to 500 mQ of potato shochu distillation waste liquid, stirred at 50°C for 20 hours, and then filtered under a filtration pressure difference of 500 + mHg. and the filtration rate is about 3
Improved twice.

Example-2 Streptomyces sp. 2O-3-A was inoculated into a liquid medium (pH 5) containing yeast extract, glucose, pectin and other plant cell wall polysaccharides, and cultured with shaking at 30°C for 7 days. Thereafter, the culture was centrifuged and a supernatant containing polysaccharide degrading enzymes was collected.

Next, 500ml of potato shochu distillation waste water diluted 4 times with water
100ml of the above supernatant liquid was added to the water, stirred at 50°C for 20 hours, and then filtered under a filtration pressure difference of 500+omHg. As a result, the filtration rate was about 5% compared to the control group to which no supernatant liquid was added. Improved twice.

Example-3゜The supernatant containing polysaccharide decomposition enzymes obtained in Example-2 was
Concentrate 30 times with an ultrafiltration membrane with a molecular weight cutoff of 10,000 to obtain a concentrated crude enzyme solution.6 Next, 50% of this concentrated crude enzyme solution
Add m12 to 500 m12 of barley shochu distillation waste liquid and heat to 50°C.
After stirring for 20 hours, filtration was performed under a filtration pressure difference of 500 mmHg. As a result, the filtration rate was approximately twice as high as in the control group in which no crude enzyme solution was added.

(Effects of the Invention) According to the present invention, by adopting a very simple configuration in which distillation waste liquid is treated using polysaccharide decomposition enzymes and/or their contents, filtration treatment was difficult in the past. The remarkable effect is that the filtration treatment of distillation waste liquid can be carried out very easily.

As a result, filtrate and solids are rapidly separated on an industrial scale.

Since the filtrate separated in this way has a very low solids content, any type of wastewater treatment technology can be applied, such as activated sludge treatment technology. .

Therefore, if the enzyme treatment and the known wastewater treatment described above are combined, the treatment of distillation waste liquid, which has been extremely difficult in the past, can be easily carried out on an industrial scale, and the effect on pollution prevention will be immeasurable. There is.

Furthermore, since the solid content separated in the above manner does not contain harmful additives or coagulants, it can be recovered and used as feed or fertilizer, and in this respect, the effects of the present invention are also enhanced. is outstanding.

Agent Patent Attorney 1) Parent Male

Claims (1)

[Claims] Shochu distillation characterized by adding a culture of a polysaccharide-degrading complex enzyme-producing bacterium belonging to the genus Streptomyces and/or a treated product thereof to the shochu distillation effluent to improve the filterability of the shochu distillation effluent. How to treat wastewater.