JPH03162652A - Method and device for measuring active oxygen species - Google Patents
Method and device for measuring active oxygen speciesInfo
- Publication number
- JPH03162652A JPH03162652A JP1301768A JP30176889A JPH03162652A JP H03162652 A JPH03162652 A JP H03162652A JP 1301768 A JP1301768 A JP 1301768A JP 30176889 A JP30176889 A JP 30176889A JP H03162652 A JPH03162652 A JP H03162652A
- Authority
- JP
- Japan
- Prior art keywords
- active oxygen
- feces
- oxygen species
- fecal matter
- sample chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 239000001301 oxygen Substances 0.000 title claims abstract description 38
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims description 23
- 210000003608 fece Anatomy 0.000 claims abstract description 55
- 238000004020 luminiscence type Methods 0.000 claims description 6
- 239000003623 enhancer Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 10
- 238000005259 measurement Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 235000013372 meat Nutrition 0.000 description 5
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 210000000936 intestine Anatomy 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 239000003642 reactive oxygen metabolite Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 206010009944 Colon cancer Diseases 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000005281 excited state Effects 0.000 description 2
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 2
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 2
- 229940118019 malondialdehyde Drugs 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000005502 peroxidation Methods 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 2
- KVJHGPAAOUGYJX-UHFFFAOYSA-N 1,1,3,3-tetraethoxypropane Chemical compound CCOC(OCC)CC(OCC)OCC KVJHGPAAOUGYJX-UHFFFAOYSA-N 0.000 description 1
- RNIPJYFZGXJSDD-UHFFFAOYSA-N 2,4,5-triphenyl-1h-imidazole Chemical compound C1=CC=CC=C1C1=NC(C=2C=CC=CC=2)=C(C=2C=CC=CC=2)N1 RNIPJYFZGXJSDD-UHFFFAOYSA-N 0.000 description 1
- HUDPLKWXRLNSPC-UHFFFAOYSA-N 4-aminophthalhydrazide Chemical compound O=C1NNC(=O)C=2C1=CC(N)=CC=2 HUDPLKWXRLNSPC-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- ZWPWSXGBDMGKKS-ZDUSSCGKSA-N Cypridina luciferin Chemical compound C1=CC=C2C(C=3NC(CCCNC(N)=N)=C4N=C(C(N4C=3)=O)[C@@H](C)CC)=CNC2=C1 ZWPWSXGBDMGKKS-ZDUSSCGKSA-N 0.000 description 1
- JREHDCFHGRHVKG-ZDUSSCGKSA-N Cypridina luciferin Natural products CC[C@H](C)C1=NC2=C(CCCNC(=N)N)NC(=CN2C1=O)c3cc4ccccc4[nH]3 JREHDCFHGRHVKG-ZDUSSCGKSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 206010009887 colitis Diseases 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 150000002912 oxalic acid derivatives Chemical class 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- LFSXCDWNBUNEEM-UHFFFAOYSA-N phthalazine Chemical class C1=NN=CC2=CC=CC=C21 LFSXCDWNBUNEEM-UHFFFAOYSA-N 0.000 description 1
- 150000008442 polyphenolic compounds Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 229940074386 skatole Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 150000003548 thiazolidines Chemical class 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（技術分Ｗ’）
この発明は、活性酸素種の測定方法とその装置に関する
ものである．さらに詳しくは、この発明は、活性酸素種
が発する発光光を利用して糞便中の活性酸素種を容易に
測定することのできる方法とそのための装置に関するも
のである。DETAILED DESCRIPTION OF THE INVENTION (Technical Part W') The present invention relates to a method and apparatus for measuring active oxygen species. More specifically, the present invention relates to a method and apparatus for easily measuring active oxygen species in feces using luminescent light emitted by active oxygen species.
（背景技術）
従来より、活性酸素種は、体細胞に作用した場合に細胞
核の遺伝子を損傷させて細胞の癌化や突然変異を誘発し
たり、細胞膜の過酸化を通じて細胞の癌化や炎症を請発
したりする物質であることが知られている。糞便中にお
いては、食物、特に脂肪含量の高い肉類や油脂の消化過
程で生ずる活性酸素種や腸肉細菌が産土する活性酸素種
が大腸癌やその他の大腸の炎症の主たる要因の一つとな
っている。そのため、糞便中の活性酸素種の測定は、大
腸癌や大腸の炎症の防止等の面から重要になってきてい
る。(Background technology) Conventionally, reactive oxygen species have been known to damage genes in the cell nucleus when acting on somatic cells, inducing cell canceration and mutations, and to induce cell canceration and inflammation through peroxidation of cell membranes. It is known that it is a substance that can be used for a long time. In feces, active oxygen species generated during the digestion of food, especially meat and oils with high fat content, and reactive oxygen species produced by intestinal bacteria are one of the main causes of colorectal cancer and other inflammation of the large intestine. There is. Therefore, measurement of active oxygen species in feces has become important from the viewpoint of preventing colon cancer and inflammation of the colon.
このような糞便中の活性酸素種の測定法とじてはずでに
これまでにチオバルビッール酸法（ Ｔ’ Ｂ Ａ法）
が提案されている。The conventional method for measuring active oxygen species in feces is the thiobarbic acid method (T'BA method).
is proposed.
しかしながら、このＴＢ八法は、活性酸素種のもつ酸化
作用によって過酸化された脂質等の過酸化の度合いをチ
オバルビッール酸がらマロンジアルデヒドへの酸化反応
に置き換えて、さらに産土されたマロンジアルデヒドを
テトラエトキシプロパンをＩ！Ａ準物質として測定する
ものであり・、操作が煩雑なうえに、類似した梢造の物
質をも同時に測定してしまうなど、特異性の点において
ら種々の問題点を有していた。However, this TB eight method replaces the degree of peroxidation of lipids, etc., which have been peroxidized by the oxidative action of active oxygen species, with an oxidation reaction of thiobarbic acid to malondialdehyde, and further converts malondialdehyde produced in the soil. Tetraethoxypropane I! It is a method to be measured as a quasi-substance A, and it has various problems in terms of specificity, such as complicated operations and the measurement of similar substances at the same time.
（発明の目的）
この発明は、以上の通りの事情を踏まえてなされたもの
であり、糞便中の活性酸素種の量の判定を、広範な分野
の研究者か利用できる程度の容易な実験操作により、高
い特異性を持ち、短時間にかつ、客観的に行えるように
することを目的としている．
（発明の開示）
この発明は、上記の目的を実現するために、糞便が発す
る発光光を検出することを特徴とする糞便中の活性酸素
種の測定方法を提供する，また、この発明は、この糞便
中の活性酸素種の測定方法を好適に実施する装置として
、糞便、たとえばその摩砕物の溶液等を入れる試料室、
試料室内の糞便の摩砕物溶液等からの発光光を検出する
極微弱光検出器、ｆ！微弱光検出機で検出された発光量
に基づき糞便中の活性酸素種の量を判定する判断部を有
してなる糞便中の活性酸素種測定装置を提供する．
すなわち、この発明の方法は、食物、特に脂肪含量の高
い肉類や油脂が消化される過程で、また、腸内相菌が腸
内において、代謝活動を行う過程で、０２− （スーパ
ーオキシドアニオン）、’０２（一重Ｉ′ｒ！酸素）
、Ｈ２ ０ｘ （過酸化水素）、Ｈ Ｏ・ （ヒドロ
キシルラジカル）等の活性酸素種や過酸化物、その他励
起状態の生成物が産生され、その産生物に山来する発光
現象が生じることに着目してなされたものであり、食物
、特に脂肪含量の高い肉類や油脂が消化される過程で、
また、腸内細菌か腸内において代謝活動を行う過程で生
じる極微弱な発光光を検出することにより糞便中の活性
酸素種を測定するものである．
糞便中の活性酸素種が発する発光光を検出するに際して
は、その発光光の測定を、糞便そのものを対象として行
ってもよく、また、糞便を適当な緩衝液等の中で摩砕し
て得られる摩砕物溶液を対象として行ってもよい。(Objective of the Invention) This invention has been made in light of the above circumstances, and it is possible to determine the amount of active oxygen species in feces using simple experimental procedures that can be used by researchers in a wide range of fields. The aim is to have high specificity, to be able to perform it in a short time, and to be able to do it objectively. (Disclosure of the Invention) In order to achieve the above object, the present invention provides a method for measuring active oxygen species in feces, which is characterized by detecting luminescent light emitted by feces. As an apparatus for suitably carrying out this method for measuring active oxygen species in feces, a sample chamber containing feces, for example, a solution of its ground material, etc.
f! is an extremely weak light detector that detects the emitted light from the fecal matter solution in the sample chamber. An apparatus for measuring active oxygen species in feces is provided, which includes a determination unit that determines the amount of active oxygen species in feces based on the amount of light emitted by a weak light detector. That is, the method of the present invention allows the production of 02- (superoxide anion) in the process of digesting food, especially meats and oils with high fat content, and in the process of metabolic activities of intestinal flora in the intestine. ,'02 (Single I'r! Oxygen)
, H2Ox (hydrogen peroxide), H O (hydroxyl radical), and other active oxygen species, peroxides, and other products in an excited state are produced, and we focus on the fact that a luminescent phenomenon occurs in the products. During the process of digesting food, especially meats and fats with high fat content,
It also measures active oxygen species in feces by detecting extremely weak light emitted by intestinal bacteria during the process of metabolic activity in the intestines. When detecting the luminescent light emitted by active oxygen species in feces, the luminescent light may be measured using the feces itself, or it may be obtained by grinding the feces in an appropriate buffer solution. The method may also be carried out using a ground material solution as a target.
発光光か弱い場合には、糞便あるいはその摩砕物等の溶
液からの発光光を増強させるため、発光増強剤を加えた
後に発光光を検出して糞便中の活性酸素種の測定を行う
こともできる。If the emitted light is weak, it is also possible to measure active oxygen species in the feces by detecting the emitted light after adding a luminescence enhancer to enhance the emitted light from a solution such as feces or its ground material. .
このような発光増強剤としては、活性酸素種や過酸化物
、その他励起状態の物質と反応して発光する化合物であ
ればその種類に限定はなく、たとえばピロガロール、バ
ープロガリン等のポリヒドロキシフェノール類、ルミノ
ール、イソルミノール等のフタラジン誘導体、インドー
ル＃酸、スカトール、トリグトファン等のインドール話
導体、ウミホタルルシフエリン、ロフィン等のチアゾリ
ジン誘導体、ビストリクロロフエニルオキザレート等の
ようなシュウ酸誘導体等が例示される。使用される濃度
は、一般的には０．１μｇ ／ ｍｌ以上が好ましい．
この発光光を測定する装置としては、極微弱光の測定か
でき、その測定値に基づいて糞便中の活性酸素種の有無
およびその強さを測定できるものが好ましい．これによ
り特別な熟練を要ずることなく糞便中の活性酸素種を検
出、でき、その強さを測定することが可能となる．
このような装置としては、たとえば第１図に示したブロ
ック梢成を有する測定装置をあげることができる．同図
の装置は、その本体が、糞便（１）をそのままあるいは
摩砕物等の溶液として入れることができる試料室（２）
と、糞便（１）からの極微弱な発光光を検出する極微弱
光検出器（３）とを有している．この試料室（２）は、
プラスチックシャーレ等から福成できる．試料室（２）
の温度は、試料室温度コントローラ（８）により測定上
適切な一定の温度に設定できるようにする．極微弱光検
出器（３）は、糞便（１）からの極微弱な発光光をフィ
ルタ（３ａ）およびシャッタ（３ｂ）を介して受光する
光電子増倍管（３Ｃ）、および光電子増倍管（３Ｃ）に
よる受光（Ｅ号を増幅するプリアンプ（３ｄ）からなっ
ている。プリアンプ（３ｄ）には高電圧電源（４）が接
続してあり、また、増幅した受光信号を計数処理してカ
ウントするカウンタ（５）が接続してある。さらに、カ
ウンタ（５）には、そのカウンタ（５）によるカウント
が一定量以上の場合には糞便中に活性酸素種が存在する
と判定し、その活性酸素秤の強さを知らせる判断部とし
てコンピュータ（６）、およびカウントをデジタル表示
することができるモニタ（７）が接続してある。Such luminescence enhancers are not limited in type as long as they are compounds that emit light by reacting with active oxygen species, peroxides, and other substances in an excited state, such as polyhydroxyphenols such as pyrogallol and barprogalin; Examples include phthalazine derivatives such as luminol and isoluminol, indole conductors such as indole #acid, skatole, and trigtophan, thiazolidine derivatives such as Cypridina luciferin and lophine, and oxalic acid derivatives such as bistrichlorophenyl oxalate. Ru. The concentration used is generally preferably 0.1 μg/ml or higher. The device for measuring this emitted light is preferably one that can measure extremely weak light and, based on the measured value, the presence or absence of active oxygen species in feces and its intensity. This makes it possible to detect active oxygen species in feces and measure their strength without requiring special skill. An example of such a device is a measuring device having a block topology as shown in FIG. The main body of the device shown in the figure is a sample chamber (2) into which feces (1) can be placed either as is or as a solution such as ground material.
and an extremely weak light detector (3) that detects extremely weak emitted light from feces (1). This sample chamber (2) is
It can be made from plastic petri dishes etc. Sample room (2)
The temperature of the sample chamber can be set at a constant temperature suitable for measurement using the sample chamber temperature controller (8). The ultra-weak light detector (3) includes a photomultiplier tube (3C) that receives ultra-weak emitted light from the feces (1) via a filter (3a) and a shutter (3b), and a photomultiplier tube (3C). It consists of a preamplifier (3d) that amplifies the light received (No. A counter (5) is connected to the counter (5).Furthermore, if the count by the counter (5) exceeds a certain amount, it is determined that active oxygen species are present in the feces, and the active oxygen scale is connected to the counter (5). A computer (6) is connected as a judgment unit to notify the strength of the count, and a monitor (7) capable of digitally displaying the count is connected.
このような装置によれば、糞便からの発光光に基づき、
糞便中の活性酸素種を高い特異性で、容易に、かつ、客
観的に測定ずることが可能となる。According to such a device, based on the light emitted from feces,
It becomes possible to easily and objectively measure active oxygen species in feces with high specificity.
以下、この発明を実施例に基づいて具体的に説明する。Hereinafter, this invention will be specifically explained based on examples.
（実施例１）
排便２時間以内のマウスの糞便の発する極微弱光を、糞
便をその湿重量の３００倍量のリン酸８衝液（ ５ ０
ＩＤＨ， ｐＨ ７．４）中で摩砕して得られた摩砕
物の溶液に対して測定した．測定にあたっては、試料室
（図１の（２））として、市販されているプラスチック
シャーレ（内径３５ｍ＋）を用い、この試利室に、上記
の糞便摩砕物の溶液を３ｍｌ注入して測定に供した．
また、糞便の発する［！微弱光が活性酸素種に由来する
ものであることを確認する目的で、活性酸素種の消去剤
である、スーパーオキシドジスムターゼとカタラーゼの
混合液を添加して上記と同様の操作を行い、その発光光
を測定した。(Example 1) Extremely weak light emitted by mouse feces within 2 hours of defecation was applied to feces in a phosphoric acid solution (50
It was measured on a solution of the ground material obtained by grinding in IDH, pH 7.4). For the measurement, a commercially available plastic Petri dish (inner diameter 35 m+) was used as the sample chamber ((2) in Figure 1), and 3 ml of the above solution of the ground feces was poured into this sample chamber and used for measurement. did. In addition, feces emit [! In order to confirm that the weak light originates from active oxygen species, we added a mixture of superoxide dismutase and catalase, which is a scavenger for active oxygen species, and performed the same procedure as above, and observed the luminescence. The light was measured.
この場合、極微弱光の測定は、第１図に示した測定装置
を使用し、その試料室温度を３７゜Ｃとした．また、測
定に際しては、雑音レベル（バックグラウンドあるいは
ダークカウント）と糞便摩砕物の溶液からの発光光とを
交互にそれぞれ２０分間ずつ測定するという操作を６回
繰返すことにより、試料からの正味の発光量を測定した
。In this case, extremely weak light was measured using the measuring device shown in Figure 1, with the sample chamber temperature set at 37°C. In addition, when measuring, the noise level (background or dark count) and the emitted light from the solution of fecal matter were alternately measured for 20 minutes each, and by repeating this procedure six times, the net emitted light from the sample was measured. The amount was measured.
その結果を表１に示した．
この表１に示した結果から明らかなように、活性酸素種
が存在している場合には有意に高い発光量を示すことが
確認される。The results are shown in Table 1. As is clear from the results shown in Table 1, it is confirmed that the amount of luminescence is significantly higher when active oxygen species are present.
表１
（実施例２）
糞便中に検出される活性酸素種には、食物、特に脂肪含
量の高い肉類や油脂の消化過程で生ずるものと、腸内細
菌が産生ずるものとがあるが、このうち、腸内相閑の産
生ずるものがどの程度の比率を占めるのかを知る目的で
、通常環境化で飼育されたマウスの糞便の発する極微弱
光と、完全無菌項境下で飼育され、腸内細菌が存在しな
いマウスの糞便の発ずる極微弱光とを、糞便をその湿重
量の１００倍量のリン酸緩衝冫夜（ ５ ０ １Ｈ，
ｐｌｌ７．４ ）中で、摩砕して得られた摩砕物の′／
８液に対して測定した．
測定に際しては、試料室（図１の（２））に注入ずる糞
便摩砕！Ｉ！Ｉ溶液の量を２ ｍｌとした以外は、」一
記実施例１と同様の測定を行った．その結果を表２に示
した。Table 1 (Example 2) Reactive oxygen species detected in feces include those generated during the digestion of food, especially meat and oils with high fat content, and those produced by intestinal bacteria. In order to find out how much of this is produced by the intestines, we exposed them to the extremely weak light emitted by the feces of mice raised in a normal environment, and the intestines raised under completely sterile conditions. The extremely weak light emitted by the mouse feces, which does not contain any internal bacteria, was mixed with 100 times its wet weight in phosphate buffer (50 1H,
pll7.4), the ground material obtained by grinding '/
Measurements were made for 8 liquids. During measurement, fecal matter is ground up by injecting it into the sample chamber ((2) in Figure 1)! I! The same measurements as in Example 1 were carried out except that the amount of I solution was changed to 2 ml. The results are shown in Table 2.
表２
糞便中に検出される活性酸素種には、食物、特に脂肪含
量の高い肉類や油脂の消化過程で生ずるものと、閣内細
菌が産生ずるものとがあるが、このうち、腸肉細閑の産
土するものが３分の２程度の比率を占めることが確認さ
れた。Table 2 Reactive oxygen species detected in feces include those generated during the digestion process of food, especially meat and oils with high fat content, and those produced by bacteria. It was confirmed that about two-thirds of the products were produced locally.
（発明の効果）
以上訂述した通り、この発明の方法によれば、ＩＪ：便
中の活性酸素種の量の判定を、広範な分野の研究者が利
用できる程度の容易な実験操作により、品い特異性を持
ち、短時間に、かつ、客観的に行うことが可能となる．
特に、この発明の装置を使用した場合には、一層容易、
かつ、確実に、糞便中の活性酸素種が検出でき、その強
さを測定することが可能となる。(Effects of the Invention) As explained above, according to the method of the present invention, the amount of active oxygen species in IJ (feces) can be determined by easy experimental operations that can be used by researchers in a wide range of fields. It has quality and specificity, and can be done in a short time and objectively.
In particular, when using the device of this invention, it is easier to
Moreover, it becomes possible to reliably detect active oxygen species in feces and measure their strength.
第１図は、この発明の方法を実施するのに好適な糞便中
の活性酸素種測定装置のブ１７ツク構成図である。
１・・・糞便
２・・・試 料 室
３・・・極微弱光検出器
３ａ・・・フ ィ ル タ
３ ｂ・・・シ ャ ッ タ
３ｃ・・・光電子増倍管
３ｄ・・・プリアンプ
４・・・高電圧電源
５・・・カ ウ ン タ
６・・・コンピュータ
７・・・モ ニ タ
８・・・試料室温度コントローラーFIG. 1 is a block diagram of an apparatus for measuring active oxygen species in feces suitable for carrying out the method of the present invention. 1... Feces 2... Sample chamber 3... Extremely weak light detector 3a... Filter 3 b... Shutter 3c... Photomultiplier tube 3d... Preamplifier 4...High voltage power supply 5...Counter 6...Computer 7...Monitor 8...Sample chamber temperature controller
Claims (1)
糞便中の活性酸素種の測定方法。（２）糞便の摩砕物溶
液からの発光光を検出する請求項（１）記載の活性酸素
種の測定方法。 （３）糞便の摩砕物溶液に発光増強剤を加えた後に発光
光を検出する請求項（１）記載の活性酸素種の測定方法
。 （４）糞便を入れる試料室、試料室内の糞便からの発光
光を検出する極微弱光検出器、極微弱光検出器で検出さ
れた発光量に基づき糞便中の活性酸素種の量を判定する
判断部を有してなる糞便中の活性酸素種の測定装置。[Scope of Claims] (1) A method for measuring active oxygen species in feces, which comprises detecting luminescent light emitted by feces. (2) The method for measuring active oxygen species according to claim (1), wherein luminescent light from a solution of ground feces is detected. (3) The method for measuring active oxygen species according to claim (1), wherein the emitted light is detected after adding a luminescence enhancer to the ground feces solution. (4) Determine the amount of active oxygen species in the feces based on the sample chamber containing feces, the extremely weak light detector that detects the light emitted from the feces in the sample chamber, and the amount of luminescence detected by the extremely weak light detector. A device for measuring active oxygen species in feces, comprising a determination section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1301768A JPH03162652A (en) | 1989-11-20 | 1989-11-20 | Method and device for measuring active oxygen species |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1301768A JPH03162652A (en) | 1989-11-20 | 1989-11-20 | Method and device for measuring active oxygen species |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03162652A true JPH03162652A (en) | 1991-07-12 |
Family
ID=17900941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1301768A Pending JPH03162652A (en) | 1989-11-20 | 1989-11-20 | Method and device for measuring active oxygen species |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03162652A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019113375A (en) * | 2017-12-22 | 2019-07-11 | サッポロホールディングス株式会社 | Method of evaluating intestinal environment, method of evaluating risk of developing colitis, and method of screening for intestinal environment improving substances |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57149950A (en) * | 1981-03-11 | 1982-09-16 | Horiba Ltd | Method for determination of hydrogen peroxide |
| JPS62250340A (en) * | 1986-04-24 | 1987-10-31 | Meidensha Electric Mfg Co Ltd | Method and reagent for hydrogen peroxide analysis by chemiluminescence method |
| JPH01269054A (en) * | 1988-04-20 | 1989-10-26 | Nippon Oil & Fats Co Ltd | Method of determining peroxide material |
-
1989
- 1989-11-20 JP JP1301768A patent/JPH03162652A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57149950A (en) * | 1981-03-11 | 1982-09-16 | Horiba Ltd | Method for determination of hydrogen peroxide |
| JPS62250340A (en) * | 1986-04-24 | 1987-10-31 | Meidensha Electric Mfg Co Ltd | Method and reagent for hydrogen peroxide analysis by chemiluminescence method |
| JPH01269054A (en) * | 1988-04-20 | 1989-10-26 | Nippon Oil & Fats Co Ltd | Method of determining peroxide material |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019113375A (en) * | 2017-12-22 | 2019-07-11 | サッポロホールディングス株式会社 | Method of evaluating intestinal environment, method of evaluating risk of developing colitis, and method of screening for intestinal environment improving substances |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dikkeboom et al. | Hemocytes of the pond snail Lymnaea stagnalis generate reactive forms of oxygen | |
| JPS5991900A (en) | Selective measurement of body cell and microbial cell havingliving activity | |
| JP5188311B2 (en) | Measuring method and measuring apparatus for biologically active substances derived from living organisms | |
| Campanella et al. | Comparison of fluorimetric, voltammetric and biosensor methods for the determination of total antioxidant capacity of drug products containing acetylsalicylic acid | |
| CN108398406B (en) | Biosensor for detecting uracil glycosylase (UDG) and application thereof | |
| CN111944738A (en) | Porcine epithelial cell oxidative stress model and establishing method and application thereof | |
| JPH03162652A (en) | Method and device for measuring active oxygen species | |
| JPH09159671A (en) | Method and equipment for measuring components derived from microorganism | |
| CN109342341A (en) | A method of utilizing carbon quantum dot Visual retrieval concentration of hydrogen peroxide | |
| NO308545B1 (en) | Procedure for rapid detection of fungal infections, as well as a kit for use in such a method | |
| KR20110043888A (en) | Device for measuring ability of anti-oxidation and method of the same | |
| CN115266664B (en) | Method for evaluating in-vitro killing efficacy of immune cell therapeutic preparation and application thereof | |
| US3575812A (en) | Method for the detection of virus | |
| US3575811A (en) | Method for the detection of cancer | |
| JPH0736013B2 (en) | Reagent for occult blood detection | |
| JP3176605B2 (en) | Method and apparatus for measuring mutagenic activity | |
| Feys et al. | Quantitative and functional requirements for bioluminescent cancer models | |
| RU2238554C1 (en) | Method for determining total antioxidation activity of biologically active substances | |
| Frérart et al. | Vascular caveolin deficiency supports the angiogenic effects of nitrite, a major end product of nitric oxide metabolism in tumors | |
| US3637655A (en) | Method for extracting water-soluble substances from biological materials | |
| CN108815504B (en) | Application of haliotis discus hannai polypeptide HDP-1 in preparation of drug for treating fibrosarcoma | |
| CN107447001A (en) | Detect pseudomonas aeruginosa and ExoS primer, kit and method in water | |
| US3432487A (en) | Process for extraction and concentration of hydrophilic substances | |
| CN109762869A (en) | A method of Salmonella in Food is detected using ATP bioluminescence reaction | |
| Zhang et al. | Tumor-targeted bioactive nanoprobes visualizing of hydrogen peroxide for forecasting chemotherapy-exacerbated malignant prognosis |