JPH03149517A - Detergent for contact lens - Google Patents

Abstract

PURPOSE:To efficiently remove the stains sticking to a contact lens without damaging the lens characteristics by combing specific proteolytic enzymes and surfactants. CONSTITUTION:The detergent is prepd. by incorporating both of the proteolytic enzyme derived from Streptomyces and the proteolytic enzyme derived from Trichilacium together with the surfactants into the detergent. The detergent consists essentially of two kinds of these proteolytic enzyme and the washing effect is synergistically improved by this combination. Any of an amphoteric surfactant and anion surfactant or both are combined as the surfactants. The amphoteric and anion surfactants do not hinder the enzyme activity of the two kinds and exhibit the washing effect without impairing the optical characteristics of the lens. Further, the washing effect is additionally improved by the combination with two kinds of the enzymes.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a contact lens cleaning agent containing a proteolytic enzyme and a surfactant.

[Prior Art] Contact lenses are generally classified into hard types and soft types.

Soft contact lenses, especially those with a high water content, are comfortable to wear, but they tend to get dirty, and washing them with water alone is insufficient, so the use of a cleaning agent is essential. ``Although hard contact lenses are less comfortable to wear than soft contact lenses, it has been said that they are easier to clean and store because they are less likely to attract dirt.However, recently, With the development and popularization of hard contact lenses that exhibit high oxygen permeability, the comfort of wearing these lenses has improved, but there have been concerns about the same problem of dirt adhesion as with soft contact lenses.

Dirt on contact lenses is at a mild stage of contamination,
That is, most of it can be removed by cleaning with a surfactant, abrasive, etc. when putting on and taking off contact lenses every day. However, if this daily cleaning is neglected, or depending on the wearer's constitution, the lens may become stuck to the lens.
Enzyme treatment using proteolytic enzymes etc. is required.

Enzyme agents added to contact lens cleaning agents include:
For example, pancreatin, trypsin, bromelain,
Proteolytic enzymes such as papain are known.
0-31834, 50-64303, 53=478
10, 54-1411553, 6G-12141
7, 62-250413, etc.).

[Problems to be Solved by the Invention] Enzymatic cleaning has a strong effect on stains that have not been subjected to physical or chemical action. However, in soft contact lenses, which are required to be sterilized by boiling, the proteins are thermally denatured and intricately intertwined, and the current state is that the removal effect on these denatured and coagulated proteins is not sufficient. .

Therefore, conventional enzyme-containing contact lens cleaning solutions contain various additives in order to enhance the cleaning effect of the enzyme, which is the main component, but the effects are not so great. For example, it is known to use urea, hydrosulfohydryl compounds, acids, alkalis, etc. However, due to the nature of urea being poorly soluble in water, it is not possible to add urea at a high enough concentration to enhance the cleaning effect, and there is also an odor. There are many problems such as accompanying.

Other additives also have limited effectiveness, and as a result, the fact is that the effectiveness of removing dirt from contact lenses depends mostly on the decomposition ability of the enzyme itself.

In addition, since the dirt adhering to contact lenses is dirt containing lipids, etc., it is possible to effectively use surfactants, but surfactants often deactivate enzyme activity, and especially soft lenses For contact lenses, there is a risk of adversely affecting the dimensions of the lenses.

Therefore, in conventional products, the concentration of surfactant has to be extremely low, and it acts more as an emulsifier than as a cleaning agent.
It merely played a role as a dispersant.

SUMMARY OF THE INVENTION An object of the present invention is to solve the above-mentioned problems of the prior art and to provide a cleaning agent that can effectively remove dirt adhering to contact lenses without impairing lens properties.

[Means for Solving the Problem] As a result of intensive research to achieve the above object, the present inventors discovered that excellent detergency can be achieved by combining specific proteolytic enzymes, and the present invention has been developed. completed. We have also discovered a surfactant that effectively acts as a detergent when used with these specific proteolytic enzymes.

That is, the contact lens cleaning agent of the present invention is derived from Streptomyces (SHeptom7ces) (1)
Proteolytic enzymes and tritylacium (Ttititx)
This product contains both proteolytic enzymes derived from chiw+*) along with a surfactant. The concentration of these enzyme agents can be 0.005.20% by weight, but is preferably 8.1-10% by weight. 0.005% by weight
If the amount is less, the cleaning effect will not be sufficient, and if it is more than 20% by weight, it is unfavorable from the viewpoint of solubility. The blending ratio of the two types of enzymes is not particularly limited, but is 1.0 to the other.
-10 times the range is preferable. Outside this range, the synergistic effect of using the two types of enzymes decreases, which is not preferable.

Preferred enzymes as proteolytic enzymes derived from Streptomyces include Streptomyces griseus (Strept.
omyc@@Hticens), Streptomyces coespi
The former is derived from, for example, actinase E1 manufactured by Kaken Pharmaceutical Co., Ltd., protease type XXI manufactured by Sigma Chemical Company, and protease type XXI manufactured by Wako Pure Chemical Industries, Ltd.
For example, protease type 1 manufactured by Sigma Chemical Company can be used as the latter.

In addition, a preferable enzyme as a protease derived from Trityracium is Trityracium album (Trit.
irxcbi++milb+v),
For example, proteinase K manufactured by Wako Pure Chemical Industries, Ltd. can be used.

In addition to the above two types of enzymes, the contact lens cleaning agent of the present invention may contain other proteolytic enzymes such as subtilisin, thermolysin, and ficin, if necessary.

The surfactant is preferably an amphoteric surfactant and/or an anionic surfactant, and the present invention is also characterized in that this surfactant is combined with the two types of enzymes.

As the surfactant, the following general formula (I) or (II) is used.
An amphoteric surfactant represented by, and the following general formula (IN)
Anionic surfactants represented by are preferred.

CH,Coo" (wherein, R is an alkyl group having an average carbon number of 9 to 15, R2, R is a methyl group or an ethyl group, and n represents 2 or 3.) CH.

R, N・-CH2Cooθ (II) ■ CH.

(In the formula, R4 represents an alkyl group having an average carbon number of 9 to 15.) Rb O u 0 (■) (In the formula, R5 is an alkyl group having an average carbon number of 9 to 15, and R6 is hydrogen or It is a methyl group, and AO has 2 carbon atoms.
~3 oxyalkylene group, m is an integer of O~20, M1. M2 represents an ion selected from the group consisting of hydrogen ions, alkali metal ions, ammonium ions, and hydroxyalkylammonium ions. ) The concentration of the surfactant to be added can be from 0.005 to 10% by weight, but preferably from 0.05 to 5% by weight. If it is less than 0.005% by weight, it is not sufficient to enhance the cleaning effect, and if it is more than 10% by weight, it may cause irritation to the eyes, which is not preferable.

In addition to the above-mentioned enzymes and surfactants, the contact lens cleaning agent of the present invention contains lipolytic enzymes, mucin-degrading enzymes, and additives such as enzyme activators, stabilizers, buffers, preservatives,
A solubility promoter, an affinity agent, etc. can also be added.

The buffer is preferably Tris-HCl buffer, usually 0.01~
It is used in a range of 5% by weight.

[Function] The contact lens cleaning agent of the present invention contains two specific types of proteolytic enzymes as essential components, and the combination synergistically improves the cleaning effect.

Conventional cleaning agents of this type often contain a single enzyme agent, and the expression pattern of enzyme activity for conventional products is 1.
It is thought that there are two peaks. Although it is not clear what causes the synergistic improvement in the cleaning effect of the present invention, it is possible that it is based on multiple activity expression patterns (persistence of peak activity, diversification of decomposition mechanisms, etc.) It is presumed that this contributes to decomposition.

In addition, amphoteric surfactants and anionic surfactants do not inhibit the activities of the above two types of enzymes, nor do they impair the optical properties of the lens, and exhibit cleaning effects. The combination can further improve the cleaning effect.

[Examples] Examples of the present invention are shown below, but the present invention is not limited to these Examples.

In the following Examples and Comparative Examples, soft contact lenses were stained using artificial stain solutions having the compositions shown in Table 1. Contamination was carried out by repeating adhesion and drying.

Table 1 Albumin ◎, s=globulin
0.2 mucin
O,Sg lecithin 0.5++ lysozyme 0.5g calcium hydrogen phosphate 0.5g physiological saline 1
001 The state of dirt is based on the following criteria.

+++: Extremely high ++: Many +: Slightly high ±: Slightly - 2 Completely removed In Tables 2 to 5, the manufacturers of enzyme preparations are as follows.

01 Kaken Pharmaceutical Co., Ltd. ゜2 Wako Pure Chemical Industries, Ltd. 113 Sigma Chemical Company (Example 1) A cleaning solution was prepared with the composition shown in Table 2, and a soft contact lens contaminated with the artificial stain solution was immersed in it. death,
It was left at room temperature for 30 minutes. The state of dirt on the lens taken out was observed using a stereomicroscope, and the results are shown in the lower column of Table 2.

Table 2 11 Ingredients 1 Example 11 Comparative Example 11 Comparative Example 21 Enzyme agent (mg) Group Actinase E” Ill 20
Proteinase K" 10-20 l Tris-HCl buffer 1...I...
,l ,,,1 knot before processing ++10+
＋10＋＋＋After 3Q minutes 10 ＋＋＋＋ (Example 2) A cleaning solution was prepared with the composition shown in Table 3, and a soft contact lens contaminated with the artificial stain solution was immersed in it.
It was left at room temperature for 30 minutes. The state of dirt on the lens taken out was observed using a stereomicroscope, and the results are shown in the lower column of Table 3.

Table 3 Group Enzyme agent (mg) Protease type IV” 10 2f11
1 buffer solution (mf) l l l
Before treatment +++ +++ 10 + + 30 minutes after 10 + + 10 (comparative example) A cleaning solution was prepared with the composition shown in Table 4, and a soft contact lens contaminated with the artificial stain solution was immersed in it.
It was left at room temperature for 30 minutes. In Table 4, the enzyme agent thermolysin is derived from Bacillus.

Observe the state of dirt on the removed lens using a stereomicroscope.
The results are shown in the lower column of Table 4.

Table 4 Thermolysin 1■20 Buffer (mt) Tris-HCl buffer 1.0 1.8 1
．． 0 result Before processing +++ + +++ +++ result
30 minutes later 10+ ++
10+ (Example 3.4) A cleaning solution was prepared with the composition shown in Table 5, and a soft contact lens contaminated with the artificial stain solution was immersed in it,
It was left at room temperature for 30 minutes. The state of dirt on the lens taken out was observed using a stereomicroscope, and the results are shown in the lower column of Table 5.

1 Yukize ltE ml (Example 5.6) Using a cleaning solution with the composition shown in Table 6 and two types of commercially available products, soft contact lenses contaminated by wearing were immersed in these and left at room temperature for 2 and 6 hours. The state of dirt on the lens after the cleaning was observed using a stereomicroscope, and the results are shown in the lower column of Table 6.

lcol l l 11
<l l l 16> =++1 羾 −-+ Kaji1唖1 l 1+ 17 ×
¥8 11! i! 1=g ls=warm 1 [Effects of the Invention] As explained above, the contact lens cleaning solution of the present invention has excellent properties against dirt adhering to contact lenses by combining two specific types of proteolytic enzymes. Moreover, by combining these with a specific surfactant, it is possible to improve the cleaning effect of the gorge layer without affecting the enzyme activity.

Patent applicant Seed Co., Ltd. □ Procedural amendment Commissioner of the Japan Patent Office Fumi Yoshida 1, Case indication Patent application No. 289376, 1999 No. 2-40-2 Hongo, Bunkyo-ku, Tokyo Seed Co., Ltd. Representative Yasuji Sejima 4, Agent 2-14-1 Shimogo, Shinjuku-ku, Tokyo 151 Telephone 951-1181 Toyu;'X + (5960) Patent attorney Satoru Yoshimura
5. Date of amendment order Voluntary amendment 6. Subject of amendment [Details of the invention 7.? Same meaning as i stop ) 3131 & filled in [Contents of amendment] rcoespitosws on page 7, line 12 of the specification
J Correct the certain description to r c*espjtosusJ.

(that's all)

Claims (3)

[Claims] (1) A contact lens cleaning agent comprising a Streptomyces-derived protease, a Trityracium-derived protease, and a surfactant. (2) The contact lens cleaning agent according to claim (1), wherein the surfactant is an amphoteric surfactant and/or an anionic surfactant. (3) According to claim (1) or (2), the surfactant is one or more surfactants selected from the group of general formulas (I) to (III) below. contact lens cleaning agent. ▲There are mathematical formulas, chemical formulas, tables, etc.▼(I) (In the formula, R_1 is an alkyl group having an average carbon number of 9 to 15, R_2 and R_3 are a methyl group or an ethyl group, and n represents 2 or 3. ) ▲There are mathematical formulas, chemical formulas, tables, etc.▼(II) (In the formula, R_4 represents an alkyl group with an average number of carbon atoms of 9 to 15.) ▲There are mathematical formulas, chemical formulas, tables, etc.▼(III) (In the formula , R_5 is an alkyl group having an average carbon number of 9 to 15, R_6 is hydrogen or a methyl group, AO is an oxyalkylene group having 2 to 3 carbon atoms, m is an integer of 0 to 20, and M_1 , M_2 represents an ion selected from the group consisting of hydrogen ions, alkali metal ions, ammonium ions, and hydroxyalkylammonium ions.)