JPH0262822B2 - - Google Patents
Info
- Publication number
- JPH0262822B2 JPH0262822B2 JP54133456A JP13345679A JPH0262822B2 JP H0262822 B2 JPH0262822 B2 JP H0262822B2 JP 54133456 A JP54133456 A JP 54133456A JP 13345679 A JP13345679 A JP 13345679A JP H0262822 B2 JPH0262822 B2 JP H0262822B2
- Authority
- JP
- Japan
- Prior art keywords
- measurement
- absorbance
- change
- value
- late
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000005259 measurement Methods 0.000 claims description 68
- 238000002835 absorbance Methods 0.000 claims description 51
- 102000004190 Enzymes Human genes 0.000 claims description 45
- 108090000790 Enzymes Proteins 0.000 claims description 45
- 230000000694 effects Effects 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 12
- 210000002966 serum Anatomy 0.000 claims description 10
- 238000011481 absorbance measurement Methods 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 5
- 238000003028 enzyme activity measurement method Methods 0.000 claims description 4
- 238000007086 side reaction Methods 0.000 claims description 3
- 238000006911 enzymatic reaction Methods 0.000 description 10
- 238000012545 processing Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000007795 chemical reaction product Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
- G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Mathematical Physics (AREA)
- Theoretical Computer Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13345679A JPS5657937A (en) | 1979-10-15 | 1979-10-15 | Measuring device of enzyme activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13345679A JPS5657937A (en) | 1979-10-15 | 1979-10-15 | Measuring device of enzyme activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5657937A JPS5657937A (en) | 1981-05-20 |
| JPH0262822B2 true JPH0262822B2 (US07696358-20100413-C00002.png) | 1990-12-26 |
Family
ID=15105198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13345679A Granted JPS5657937A (en) | 1979-10-15 | 1979-10-15 | Measuring device of enzyme activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5657937A (US07696358-20100413-C00002.png) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59116549A (ja) * | 1982-11-30 | 1984-07-05 | 日本テクトロン株式会社 | 自動分析装置における分析デ−タのモニタ−方法及びその装置 |
| JPS6181774A (ja) * | 1984-09-29 | 1986-04-25 | Shimadzu Corp | 酵素活性測定装置 |
| JP2564788B2 (ja) * | 1984-11-27 | 1996-12-18 | 株式会社島津製作所 | 経時的変化の分光的測定装置 |
| CN101936909B (zh) * | 2010-08-31 | 2012-05-23 | 天津工业大学 | 一种微生物降解苯环的酶活测试方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4918395A (US07696358-20100413-C00002.png) * | 1972-04-17 | 1974-02-18 |
-
1979
- 1979-10-15 JP JP13345679A patent/JPS5657937A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4918395A (US07696358-20100413-C00002.png) * | 1972-04-17 | 1974-02-18 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5657937A (en) | 1981-05-20 |
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