JPH0249709A - Melanin formation inhibitor containing logwood extract - Google Patents

Abstract

PURPOSE:To obtain a melanin formation inhibitor expected to have anti-suntan effects in blending the inhibitor with a skin cosmetic and using containing a specific extracted essence obtained from logwood, haematein or haematoxylin. CONSTITUTION:A melanin formation inhibitor which contains an extract (containing isolated substance) of logwood, an essence extracted with water or mixed solution of water and ethanol or water and methanol or haematein or haematoxylin shown formula I-II as an active ingredient, can protect skin from sunlight, suppress blackening phenomena of skin and prevent pigmentation such as stain and freckle and can be applied to drug field, plant tissue culture, growth controlling agent (rooting or germination) in garden plants, etc., fermentation industry field, etc.

Description

[Detailed description of the invention]

[1] Purpose of the invention The present invention uses legumes.

[Related to] A melanin production inhibitor comprising a specified extract (containing a tunic substance) obtained from the heartwood of Goumed. Fields of application of the extract according to the present invention include skin preparation preparations, external skin preparations (hereinafter, internal application will simply be referred to as skin preparations, skin preparations, etc.).
When used in combination with F-1 fire, it can be expected to have a 1F prevention effect. Further, as specific skin cosmetics, it can be used by being incorporated into creams, milky lotions, lotions, and liquid type formulations. 1 Conventional technology. Substances that inhibit tyrosinase activity are used for the purpose of preventing skin sunburn and maintaining beautiful skin. Furthermore, in order to prevent sunburn, materials that absorb ultraviolet rays and visible light are required. Furthermore, regarding the extract obtained from logwood, we searched for publications on the use of this in skin cosmetics, but found no other publications. In addition, there are examples where it is used in skin cosmetics for the purpose of preventing sunburn or as an ultraviolet absorber, or
It was also completely unknown that it had a tyrosinase activity suppressing (inhibiting) effect. However, hematei obtained from logwood is known as one of the raw materials for hair dyes, for example,
Its standard value is shown in the accompanying publication (1) below, and furthermore, the means for obtaining hematin is shown in publications (2) to (3) below. Also, recently, hair dyes using hematin are commercially available. (Location of publicly known publications) (1) Hair dye raw material standards (Pharmaceutical research document 5) 9.5 Edited by Japan Official Publications Association, published December 1974 (2) The Journal of the Society of Dyers and Colorists ( The Journal of
the 5ociety of Dyers andC
olourists) Volume 75 November issue 9.51
3 Published in 1959 (3) Same as above, Vol. 70, September issue 9. 392 Published in 1954 (4) Chunichi Shimbun p., 12 Advertisement Column 1 "Kuroreika" 1988
Published on July 30, 1988 Applications to hair dyes include hematin or hematoxylin contained in logwood.
This method takes advantage of the effect of ``Problem to be solved by the invention'' The problem of the present invention is that the original purpose was to
High-quality hematin with little ignition residue has the disadvantage of having a large amount of ignition residue, so a hair dye manufacturer requested that it be purified based on 17tein for commercial use. . It was desired that the required ignition residue should be at least 5% or less. However, when conventional purification methods are used based on commercially available hematin, losses occur during purification and additional washing operations are required several times, resulting in high costs. In other words, the ignition residue of hematin specified in the hair dye raw material standards is set at a much higher upper limit of 15%, reflecting the actual situation obtained based on the conventional method, and is stipulated to be less than this. Therefore, it can be said that reduction of the numerical value was left to manufacturers' independent technical means. The trigger for the present invention was to grasp this current situation, obtain hematine based on logwood, and develop it. In other words, based on the various extracts obtained in the process of examining various extraction methods, the present inventors simultaneously searched for applications of these extracts in applications other than hair dyes. At the same time, we conducted tests on physical properties, etc. As a result, as shown below, we found that each extract has a strong melanin production inhibiting effect.Based on this, we further investigated the pigments that are hair dye ingredients: hematin and hematoxylin. was found to be the main substance. In other words, the problem to be solved by the present invention is to obtain highly purified hematin, and as one of its new application fields, it is applicable not only to hair dyes but also to hair dyes.
This was successfully developed into a skin cosmetic application. In order to specifically illustrate the present invention, the present invention will be described in detail using experimental (test) methods and the like. [2] Structure of the Invention The present invention provides an extract obtained from logwood heartwood, an extract obtained using an extraction solvent as shown in Examples ■ to ■ below, or an extract as shown in Example ■. It is specified as a mennin production inhibitor containing hematoxylin or hematin obtained from logwood. 1. A means to solve a problem. Obtaining the extract used in the present invention or its Tun PJ component 4-
Based on the manufacturing method (example) shown below,
You can get a lot of customers. However, in the present invention, a commercially available reagent-grade hemadoxylin was obtained and used. (Example 1: Extraction method using water) Using chopped logwood, add purified water 71 to 1 kg, stir and extract at 95°C or higher, filter, and collect the filtrate. The residue is again subjected to the above-mentioned extraction and filtration, and the filtrate is collected and concentrated under reduced pressure until it reaches about 500 ml, and the obtained extract is used as a melanin production-inhibiting extract. (Example 2: Extraction method using organic solvent) After cutting the logwood into pieces, a mixture of 3 parts of purified water and 3 parts of methanol, or 3 parts of ethanol instead of methanol, was added to 1 kg of logwood. After adding 6 parts, immersing at room temperature, filtration, separating the filtrate, adding the above extraction solvent again to the residue, immersing, filtering, taking the filtrate, and The extract is combined with the filtrate and concentrated under reduced pressure at 50 to 60°C until it becomes a paste, and the obtained extract is used as melanin production inhibitor M. (Example 3: Extraction method of hematin) To the extract obtained in the process of Example 1, 100 ml of aqueous ammonia was added under stirring, and then 50 ml of hydrogen peroxide was added, and the mixture was left for 30 minutes. do. After leaving for 30 minutes (reaction), immediately pour in 2 to 3 times the amount of water, add an appropriate amount of methanol, concentrate under reduced pressure, dry under reduced pressure, and pulverize to obtain hematin. This substance is used to suppress melanin production. In addition, in Example 2, although the mixing ratios of water and methanol and water and ethanol were shown, this ratio is not particularly limited. 1. Confirmation of Effect: Evaluation of Melanin Production Inhibition Effect" (Test Method: Melanin Polymer Production Inhibition Test) Using pine mushroom extract tyrosinase, measurements were made under the following reaction system conditions. The results are shown in the following tables (Tables 1 to 4). (1) Reaction system Add L-thi1'gin solution 1, Q mQ, bean culpain bug fur solution 2, and OmQ to a test tube, and heat at 37°C.
Incubate for 10 minutes in a thermostatic bath, then add 0.1-ml of tyrosinase solution and react for 1 hour in a 37°C thermostatic bath. Leave at room temperature for more than 10 hours, then transfer to a small petri dish. Transfer and observe the formation of melanin polymers. Note that each solution is added at the beginning. (2) Test solution A: extract obtained in Example 1. B: Extract obtained in Example 2. C: Hematin obtained according to Example 3. D = madoxylin to commercial reagent. (3) Preparation of sample solution A is one in which 2 walls were mixed with purified water to make 1000 ml. Dissolve 0.5g of B in 50% methanol solution and add 10G
+IIt. C is tot, o■ dissolved in 50% ethanol solution, 5
011t. D is 100.0■ dissolved in 50% ethanol solution, 5
〇-Deaf. (4) Evaluation criterion I'! 11 (Symbols in Tables 1 to 4) No melanin polymer observed No clear formation of melanin polymer: ±. Melanin polymers observed: 10. “Table 1” Observation of melanin polymer [Sample: A) “Second
Table “Observation of melanin polymer (Sample 2B) 1%3 Table”
Observation of melanin polymer (sample: C) 1% Table 4 Observation of melanin polymer (sample: D) Ultraviolet absorption action Figure 1 shows the ultraviolet absorption spectrum of hematin,
In addition, although the second IyJ was measured for the absorption spectrum in the visible region, the extracts obtained in Examples 1 and 2 were also measured in the same manner with 200 to 21
It has a maximum absorption near 0n-, further increases to 270~b, and in the visible region, the absorption r
It is characterized by having t. Therefore, in addition to the above-mentioned melanin production inhibiting effect, substances with absorption ability over a wide range protect the skin from sunlight and suppress the darkening phenomenon of the skin.
It prevents pigmentation such as spots and freckles, and can be expected to have the effect of suppressing melanin production from the surface of the skin. 4. Also, 01 in the table (Tables 1 to 4) indicates that it is inside the skin, and from the absorption wave pattern shown in Figures 1 and 2, it is the surface of the skin. In both cases, sunburn prevention is 11]
The effects can be expected. "Blending! Estimated amount of apricots" When applying the extract according to the present invention, for example, when using it in skin cosmetics, as a guideline for the amount of blending, the extract obtained in Example 1 should be 0°. 04~0.1v/v%
Hereinafter, for the extract according to Example 2, 0.008 to
0.01 w/v% or less, 0 for hematin
．． 02 to 0.04 w/V% or less, in hematoxylin,
A guideline for this addition is 0.004 w/V% or less! By permeating into the epidermal keratin protein layer of the skin, Jt improves the metabolism of the epidermal layer and promotes the formation of shinjo, which is useful for suppressing age spots and wrinkles, in other words, preventing skin aging. , for the epidermal keratin protein of the skin, '+t [1
It's also cheap. Depending on the layer of the skin cosmetic, this formulation can be used for 11, cleats, +1-nJ, packs, makeup right end, and bath M, and can be used to prevent skin aging. It is expected that this will be prevented. Hereinafter, two to three formulation examples containing the melanin production inhibitor according to the present invention will be shown; however, the present invention is not limited to these formulation examples, and can be applied to various known skin cosmetics. It can be used by mixing it with food. (Formulation example 1: cream) Beeswax...Cefnol...--...φ-Fluid polyisobutylene...Squalane...Sho...Eleventh Society/Square/Fatty acid Glycerin ester... Lanolin fist, Kanken fist, +++ e-threat, ノ■Pyrene glycol, ++1 Extract obtained in Example 1, Purified water...Fragrance, Adjacent rot... Extract obtained from Example 1 Quen C1 (1.1++ fist/fist... J r+pyrene glycol... R3C liquid... ...... ・ ・ 6 , 0% ・ ・ 5 , 5 % ・13.0% ・10.0% ・ ・ 4 , 5% ・ ・ 4.0 % ・ ・ 5.0 % ・ 0 , 04%・51,0%...Suitable Iit・0,1%・0,5%・7,0%・5,0% Purified water・・・・・・87.4 %Fragrance/preservative......Appropriate amount (Formulation example 3: Par/
ri) Hematoxylin...0.004% Veegum...6.0% Squalane...
・・・・・・・・・2.5% propylene glycerin; 7-ru・
・－・・・6.0% Zinc axide・・・・・・・・・・・・
・10゜0% kaolin・・・・・・・・・・・・9.
5% Ethanol, 6 Umbrellas, 6.0% Purified Water, 59.9% Fragrances, Preservatives, etc. ... Suitable [, [(Formulation example 4: Emulsion) Häntein...0.04% Sj-
Allic acid---------1,2% (・Tanol------2.2% SoI! Phosphorus...
・・・・・・・・・・・・2.7% Suxolane・・・・・・
・・・・・・・・・5.0% Yuka Bar 11 Oil・・・・・・
...1.5% polyoxylene 1-lene (20) sorbitan monosura'arate ...2.0% lipophilic sorbitan monostearate 1.5% glycerin
・・・・・・◆・・・・・・5.0% purified water・・・・・・
・・・・・・78.8% Fragrance and preservatives・・・・・・・・・
The expected effects of the skin cosmetics shown in the prescription examples are that when applied and rubbed on the skin, it will prevent darkening of exposed skin areas caused by light, and will also protect the face from darkening. 1. Regarding dark spots and freckles that appear on the skin, they often become darker and more noticeable than other surrounding skin colors due to light; prevent,
You can keep it light. [3] Effect of the invention The effect of the present invention is that it is used as a melanin production suppressing shaver based on logwood extract. [And, as for specific fields of use, examples are λbarrii and t-go! Contains a special ingredient to protect the skin, especially stains and freckles, or to prevent the formation of wrinkles.
It is recommended to use it when removing spots and freckles on the face. It is true that until now, it was not known that the extract obtained from L1 Guwood has a melanin production inhibiting effect, but since then,
In addition to being used as dyes, hair dyes, and coloring agents, the ability to suppress melanin production can be used not only in cosmetics for the skin described above, but also in the pharmaceutical field, for example. 1. For plant tissue culture or horticultural plants. Application of growth control (rooting, germination) agents, etc.; Application of fermentation to industrial fields (antibiotics, etc.) is l1
rf), H is considered. 4-Nawamo, living organisms (animals, plants, microorganisms),
5. Concerning acid '1 synthesis. 64-11jiL -t= no role i'il There is a insect cost ℃, especially noy, Ni 1 - Lua 7: -' 744 I + Jin, the induction to oxidized substance is that ^YA If ``+t' exists, it becomes ``C'' that causes the damage of the species/J to become ``C''. Therefore, ``r: Suitable!'' It can also be used to suppress melanin production of J'c.

[Brief explanation of the drawing]

FIG. 1 shows the ultraviolet absorption spectrum of H-Rtin. Figure 2 shows the visible absorption spectrum of hematin. Figure 3 shows the infrared absorbing surface of Machiin.

Claims (1)

[Claims] (1) Logwood extract is [1] an extract extracted using water, [2] an extract extracted using a mixture of water and ethanol, or water and methanol, [3] hematein,
[4] A melanin production inhibitor containing any one of hematoxylins [1] to [4].