JPH0226935B2 - - Google Patents

Description

[Detailed Description of the Invention] (Purpose of the Invention) This invention mass-produces virus-free seedlings by shoot tip culture, which is a type of tissue culture, and is suitable for not only specialized farmers of various horticultural crops but also general hobbyists. The purpose of this invention is to provide a propagation device with a new structure for easily growing high-quality seedlings, and a new propagation method using the same.

(Prior Art) The recent progress in biotechnology has been quite remarkable. One such technology is tissue culture, especially shoot apical culture, which enables the mass propagation of seedlings.The range of crops to which it can be applied is also expanding, and it is seen as an important technology that will support the seed and seedling industry in the future. .

For shoot apex culture, cut the bud tip of the target plant, disinfect it, dissect the bud tip under a stereomicroscope on a sterile workbench, cut the growing point to about 0.2 to 0.5 mm, and place it on an agar medium. This is a vegetative propagation method in which the shoot apex is cultivated to grow leaves and roots, and it has been confirmed to be extremely effective as a seedling production technique for vegetatively propagated crops that efficiently produces large numbers of genetically uniform cloned seedlings.

However, in order to make use of this shoot apex culture technology to increase the economic effect of the seed and seedling industry, it is necessary to gradually acclimatize the seedlings to the outside environment immediately after transplanting them into the soil, and to supply large quantities of virus-free seedlings in a short period of time. A system must be put in place to enable this, and so far, we have adopted hydroponic cultivation as a system for this purpose, as well as a biofactory that can be managed intensively, such as by completely controlling light, temperature, and humidity. It is considered that production in this field is most suitable, and therefore, the current situation is that most of the technological development related to the practical application of this shoot apex culture technology is exclusively related to that direction.

The present invention is one step ahead of such circumstances and has been involved in the development and research of a technology that can easily commercialize this shoot apex culture technology, and has finally achieved its results, and its structure is as follows: , as detailed below.

(Structure of the Invention) As clearly understood from the exemplary embodiments of the present invention shown in the drawings, the plant propagation device of the present invention has two configurations: A transplanting culture medium 2 is loaded below the cutout window 11, while a transparent container 3 is internally and housed above the cutout window 11 so as to substantially close the cutout window 11. 3 includes a closed container 4 in which shoot apex culture is performed, in which the shoot apex is placed in culture 42 under sterile conditions.
This is a plant propagation device that stably houses plants and doubles as a display and sales device.

The outer container 1 can be made of almost any structure, such as cardboard, as long as it can be appropriately assembled into a box shape, similar to so-called outer containers for general products. In addition to various printing designs to increase the value of the product, instructions on how to use the device and information on the manufacturer and seller are also included.

A cut-out window 11 is formed in the middle of this outer container 1 with a size corresponding to approximately half of the height and outer circumference of the container, and is used as a window for observation of the airtight container 3 to be described later. The box also has a window for letting in light to the plants in the container 3. This cutout window 11
Of course, the optimum cutout shape is selected from the relationship with the overall shape of the outer container 1.
It must also function satisfactorily as part of the exterior design.

As the transplanting soil 2, Kanuma soil, pumice stone, or other commonly commercially available cultivation soil is sufficient, and after sterilizing and sterilizing it and mixing in fertilizer as appropriate, a certain amount is used in bags 21. This makes it extremely easy to handle.

If necessary, as in the illustrated example, the bagged 21 culture soil 2 is stored in a simple pot 22 made of a hygroscopic material similar to particle board, for example, and placed in the pot. If the container 22 is loaded below the outer container 1, it will be even more convenient.

The transparent container 3 is made of a transparent material such as plastic, has a size that can be fitted inside the outer container 1, and has a structure in which at least one end is closed with a perforated bottom plate 31. shall consist of.

When first stored in the outer container 1, this transparent container 3 functions as a container for stably storing an airtight container 4, which will be described later, with the bottom plate 31 side facing down, and allows the purchaser to store the seedlings 6 grown after purchase. After the seedlings 6 are transplanted into the transplanting soil 2, the bottom plate 31 side is turned upward to function as a cover that temporarily blocks the young plants 6 from the outside world.
In the figure, 32 indicates a through hole formed in the perforated bottom plate 31, and 5 indicates a pedestal for stably positioning the closed container 4 within the transparent container 3.

The airtight container 4 is a container with a lid 41 made of glass or other transparent material, and is well sterilized and has a culture medium 42 (usually agar, inorganic salts, sucrose, vitamins, and auxin) inside. (added with various plant hormones such as cytokinin)
A so-called shoot apex culture is performed in which a shoot apex cut to a predetermined size is placed on the culture medium 42, and then completely sealed.

This airtight container 4 is housed in the transparent container 3 as described above, and the lower part thereof is placed on the pedestal 32.
Further, the upper portions thereof are fixed with fixing frames 13 built into the outer container 1, so that they can be stably arranged.

The device of the present invention having the configuration as described above can be used in the following steps.
It is a method of propagating plants.

First step: Light is allowed into the closed container 4 in which shoot apex culture has been performed through the transparent container 3 that closes the cutout window 11 of the outer container 1, and the stems and leaves of the virus-free seedlings 6 are observed at appropriate intervals. Allow the roots to grow fully.

2nd step When the stems, leaves, and roots of the virus-free seedlings 6 have sufficiently formed, the airtight container 4 is taken out and opened, the seedlings 6 are taken out from the container 4, and the medium 42 is carefully dropped from the roots. Wash with water.

Third step: Take out the transplanting soil 2 from the outer container 1, fill it into a flowerpot as appropriate, and transplant the aforementioned young plant 6. At the same time, take out the transparent container 3 from the outer container 1 and cover the transplanted young plant 6, The young plants 6 taken out from the airtight container 4 are managed so that they gradually get used to the outside air without being directly exposed to the outside air.

Fourth step: The transparent container 3 is completely removed, and the transplanted seedlings 6 are allowed to grow in a state where they are directly exposed to the outside air to form seedlings.

FIG. 2 shows a simple drawing of each of the above steps.

The flower pot illustrated in the third step is an example in which the attached simple pot 22 is used, but of course, this pot can be made of a regular clay pot, a plastic pot, or any other type of pot. , you can place the transplanting soil 2 in the supplied simple pot 22 and place it in a regular flower pot, and then fill the surrounding area with more soil, or you can directly put it in a regular flower pot without using the simple pot 22 at all. Needless to say, there is no particular problem in porting it.

(Operation and Effect) The propagation device of the present invention is used in the first stage, that is, by displaying and selling closed containers in which shoot apex culture has been carried out, and after purchase by a purchaser until shoots and roots are sufficiently formed. At this stage, the transplanting soil 2 (in the case of the example, further up to the simple pot 22) and the transparent container 3, which will be required later, are placed in the outer container 1, not only during display and sales, but also during the time of display and sale. The design is functional, compact, and designed so that the body of the plant does not deteriorate even during the formation of shoots and roots, and does not interfere with the observation of the process of forming shoots and roots, as well as the growth of the seedlings 6 that are cultured at the top of the shoots. Since it is designed to be effective, it is very convenient to handle for both sellers and buyers.

In addition, in the subsequent stage, that is, in the stage where the seedlings 6 that have sufficiently achieved the formation of stems, leaves, and roots are intended to grow even larger, the soil 2 necessary for transplanting is of course kept in a virus-free state. The outer container 4 includes a transparent container 3 for temporarily keeping the grown seedlings 6 in a state of indirect isolation from the outside world so that they are not directly exposed to the harsh outside world.
Since the plants are prepared in a compact state in advance, there is no need to panic in procuring the necessary materials at the transplant stage, and transplants can be carried out at the right time extremely efficiently. It has become a product with excellent characteristics.

Therefore, while the conventional propagation device required a specially prepared and expensive biofactory, the propagation device of this invention allows anyone to easily grow the shoot apex without requiring any special facilities. It becomes possible to propagate plants through culture, and the propagation and dissemination of plants that inherit only excellent properties, which has been thought to be difficult until now, becomes extremely simple and reliable.

In addition, since the method of propagating plants using this propagation device uses a device with the characteristics described above, it is possible to reliably breed excellent plants by simply carrying out an extremely simple work process. The virus-free seedlings can be raised by a wide range of people, from professional gardeners to general gardening enthusiasts.

In particular, the transplanting process after the formation of stems, leaves, and roots is a process that can be carried out without special facilities unlike previous methods. This is a big support.

As mentioned above, both the plant propagation device of this invention and the propagation method using it will substantially improve the economic efficiency of biotechnology, which has been slow to take off, and make it a valuable technology. , and its impact on industry could be extremely large.

[Brief explanation of drawings]

The drawings show representative embodiments of the present invention; FIG. 1 is a longitudinal sectional view showing the device of the present invention, and FIG. 2 shows the steps of a breeding method using the device in the order of the steps. It is a simplified process diagram. 1... Outer container, 11... Cutout window, 12...
...Same fixed frame, 2...Cultivating soil for transplanting, 21...Same bag,
22...Simple pot, 3...Transparent container, 31...
...Same hole, 4... Sealed container, 41... Same lid, 42
...Medium, 5...Pedestal, 6...Sapling.

Claims (1)

[Scope of Claims] 1. For an outer container that has a large cutout window in the middle, planting soil for transplanting is loaded below the cutout window, while above the cutout window, the cutout window is substantially closed. In this way, a transparent container is inscribed,
A plant propagation device doubles as a display and sales device, which stably houses a closed container in which a shoot apex is cultured by placing the shoot apex in a medium in a sterile state in the transparent container. 2 For an outer container that has a large cut-out window in the middle, fill soil for transplanting below the cut-out window, and place a transparent container above the cut-out window so as to substantially close the cut-out window. inscribed,
A plant propagation device that doubles as a display and sales device, in which the transparent container stably houses a closed container in which the shoot apex is cultured by placing the shoot apex on a medium in a sterile state. A breeding method that utilizes the following from the first step to the fourth step. 1st Step Light is allowed into the closed container in which the shoot apex culture has been performed through the transparent container through the cut-out window of the outer container, and the stems, leaves, and roots of virus-free seedlings are grown by observing as appropriate. 2nd Step When the virus-free seedlings have sufficiently formed stems, leaves, and roots, the sealed container is taken out and opened, the seedlings are removed from the container, and the medium is removed from the roots and washed with water. 3rd step: Take out the transplanting soil from the outer container, put it in a pot, and transplant the seedlings, take out the transparent container from the outer container, cover the transplanted seedlings, and place the seedlings taken out of the airtight container. Do not expose them directly to the outside air, but gradually let them get used to the outside air. Fourth step: The transparent container is completely removed and the transplanted seedlings are exposed directly to the outside air to grow and form seedlings.