JPH0218000A - Propagating method of microorganism for decomposing humic acid - Google Patents
Propagating method of microorganism for decomposing humic acidInfo
- Publication number
- JPH0218000A JPH0218000A JP63167830A JP16783088A JPH0218000A JP H0218000 A JPH0218000 A JP H0218000A JP 63167830 A JP63167830 A JP 63167830A JP 16783088 A JP16783088 A JP 16783088A JP H0218000 A JPH0218000 A JP H0218000A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- humic acid
- basidiomycetes
- agitation
- utilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004021 humic acid Substances 0.000 title claims abstract description 29
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 244000005700 microbiome Species 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title claims description 22
- 230000001902 propagating effect Effects 0.000 title claims description 7
- 241000221198 Basidiomycota Species 0.000 claims abstract description 32
- 238000013019 agitation Methods 0.000 claims abstract description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000008188 pellet Substances 0.000 claims abstract description 9
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 7
- 235000000346 sugar Nutrition 0.000 claims description 3
- 150000008163 sugars Chemical class 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 9
- 238000012258 culturing Methods 0.000 abstract description 7
- 241000222356 Coriolus Species 0.000 abstract description 2
- 241000223251 Myrothecium Species 0.000 abstract description 2
- 150000001720 carbohydrates Chemical class 0.000 abstract 2
- 230000000644 propagated effect Effects 0.000 abstract 2
- 241000222350 Pleurotus Species 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 238000004062 sedimentation Methods 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000006481 glucose medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000238557 Decapoda Species 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000000149 chemical water pollutant Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- -1 nitrogen-containing compound Chemical class 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Activated Sludge Processes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はフミン酸を含有する水からのフミン酸除去装置
に適用される生物学的な処理方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a biological treatment method applied to an apparatus for removing humic acid from water containing humic acid.
フミン酸は、埋立地浸出水、河川水、湖水等に含まれて
おり、高濃度で存在すると黄褐色、黒褐色を呈する。フ
ミン酸の化学構造は十分には解明されていないが、その
本質は多価フェノール型の芳香族化合物と含窒素化合物
との縮合物と言われておル、生物学的処理方法では分解
されにくい生物難分解性物質として知られている。そこ
で、従来はこのフミン酸を含む水の処理は、凝集沈殿法
や活性炭吸着法あるいはオゾン酸化法等の物理化学的方
法の利用が試みられている。Humic acid is contained in landfill leachate, river water, lake water, etc., and when present in high concentrations, it appears yellowish brown or blackish brown. Although the chemical structure of humic acid has not been fully elucidated, its essence is said to be a condensate of a polyhydric phenol-type aromatic compound and a nitrogen-containing compound, and it is difficult to decompose using biological treatment methods. It is known as a bio-resistant substance. Therefore, in the past, attempts have been made to treat water containing humic acid by using physicochemical methods such as coagulation-precipitation method, activated carbon adsorption method, or ozone oxidation method.
しかしながら、従来の物理化学的処理方法は、いずれも
肝心のフミン酸の除去率が低いという欠点があ夛、特に
高濃度の７ミンｒＲを含む水を処理する場合、凝集沈殿
法ではアルミニュム塩などの凝集剤添加を、またオゾン
酸化法ではオゾンの添加を多量に必要とするほか、活性
炭吸着法では活性炭の劣化が速くなるため、その交換頻
度や再生頻度が多くなりその処理コストが非常に高くつ
くという欠点があった。However, all of the conventional physicochemical treatment methods have the drawback that the removal rate of the essential humic acid is low.Especially when treating water containing a high concentration of 7min rR, the coagulation precipitation method In addition, the ozone oxidation method requires the addition of a large amount of ozone, and the activated carbon adsorption method deteriorates the activated carbon quickly, requiring frequent replacement and regeneration, resulting in very high processing costs. It had the disadvantage of being sticky.
本発明は従来法における欠点を解消し、高濃度のフミン
酸を含有する水であっても効率よくフミン酸を除去し、
良好な処理水を得ることのできる新規な方法を提供しよ
うとするものである。The present invention overcomes the drawbacks of conventional methods and efficiently removes humic acids even from water containing high concentrations of humic acids.
The aim is to provide a new method that can obtain good quality treated water.
この目的のため、本発明者等は全く新しい処理方法とし
て微生物を利用することに着目し、フミン酸を分解する
能力をもつ微生物の探索を行った結果、マイロセシユー
ム（Ｍｙｒｏｔｈｅｃｉｕｍ）属、プロイロトス（Ｐｌ
ｅｕｒｏｔｕｓ）属、カリオラス（Ｃｏｒｉｏｌｕｓ）
属に属する担子菌類に、その能力のあることを見い
出し、この微生物を利用したフミン酸含有水の処理法を
先に提案した。（特願昭６２−２９５１７１号）この提
案内容を簡単に要約すると、フミン酸含有水にマイロセ
シユーム属、プロイロトス属又はカリオラス属に属する
担子菌を好気的条件下で接触させることによｐフミン酸
を分解・除去するフミン酸含有水の処理法であるが、本
発明はこの担子菌によるフミン酸の分解を合目的に行え
るように、この担子菌の培養・増殖方法を改良しようと
するものである。For this purpose, the present inventors focused on the use of microorganisms as a completely new treatment method, and as a result of searching for microorganisms that have the ability to decompose humic acid, they found that Myrothecium genus, Ploylotus (Pl.
genus eurotus, Coriolus
We discovered that Basidiomycota, which belongs to the genus Basidiomycota, has this ability, and we first proposed a method for treating humic acid-containing water using this microorganism. (Japanese Patent Application No. 62-295171) To briefly summarize the content of this proposal, p-humic acid can be obtained by contacting basidiomycetes belonging to the genus Mylocesium, Proirotus, or Cariolus under aerobic conditions with humic acid-containing water. This is a method for treating water containing humic acid to decompose and remove humic acid.The present invention aims to improve the method for culturing and propagating humic acid so that the basidiomycete can decompose humic acid for the purpose. be.
すなわち、フミン酸含有水と接触させる担子菌の性状と
しては、まず、■沈降速度が速いこと。■フミン酸含有
水との接触効率を良くするため菌の表面積をなるべく多
くすること、換言すれば分散性をよくすること。がちけ
られるが、その理由は、まず沈降速度が速ければ７ミン
酸含有水の処理プロセスにおける固液分離装置、（通常
、沈殿槽と呼ばれるもの）がコンパクト化ができ、また
８Ｂ成分（Ｓｕｓｐｅｎｄｅｄ ８ｏ１１ｄ ・・・浮
遊性固形分）が少ない良好な処理水が得られるからであ
り、また、菌をよく分散させ、フミン酸含有水との接触
効率をよくすれば、当然のことながら分解効率が上がシ
、処理性の向上、反応器のコンパクト化につなげること
ができるからである。That is, the characteristics of the basidiomycete to be brought into contact with humic acid-containing water include (1) a high sedimentation rate; ■Increase the surface area of bacteria as much as possible to improve contact efficiency with humic acid-containing water, in other words, improve dispersibility. The reason for this is that firstly, if the sedimentation rate is high, the solid-liquid separator (usually called a sedimentation tank) in the treatment process of water containing 7 minic acid can be made more compact. This is because good treated water with low content (sustainable solids) can be obtained, and if bacteria are well dispersed and the contact efficiency with humic acid-containing water is improved, the decomposition efficiency will naturally increase. This is because it can lead to improvements in processability and downsizing of the reactor.
しかし、■の沈降性を速くするということは菌を分散さ
せることなく、お互い菌同志を凝集、団塊状にして菌を
大きくすることであり、■の分散性をよくするというこ
とはお互い相反する性質をも念ぜようとするものである
。However, increasing the sedimentation rate of (■) means increasing the size of the bacteria by aggregating and clumping together the bacteria without dispersing the bacteria, and improving the dispersibility (■) is contradictory to the other. It also tries to remind us of our nature.
本発明者等が見い出した担子菌社いづれも太さカニ３〜
４ ｌｔの菌糸をもら、その菌糸は長く糸状に伸長して
数百μ以上に達し、また、炭素源としてグルコースをよ
く資化することがわかっている。The Basidiomycota found by the present inventors are all crabs with a thickness of 3~
4 lt of hyphae were obtained, and the hyphae are long and filamentous, reaching several hundred micrometers or more, and are known to be able to assimilate glucose well as a carbon source.
仁の担子菌をグルコースを含む培養液で培養を行う際、
培養液を強く撮とう撹拌すると、菌糸が分散・増殖し、
沈降性が悪くなってしまう。When culturing the basidiomycete in a culture medium containing glucose,
When the culture solution is vigorously stirred, the mycelium will disperse and multiply.
Sedimentability deteriorates.
またゆるやかな振とう撹拌により培養を行うと、担子菌
は大きな団塊となって増殖し、分散性が悪くなってしま
うという不具合がある。Furthermore, if culture is carried out by gentle shaking and agitation, the basidiomycetes grow in large clumps, resulting in poor dispersibility.
分散性がよく、かつ沈降速度の速い担子菌の培養方法に
ついて徨々、検討を行った結果、本発明者らは、次のよ
うな培養方法でこの目的を達せられることを確認した。As a result of extensive research into culturing methods for basidiomycetes that have good dispersibility and a high sedimentation rate, the present inventors have confirmed that this objective can be achieved by the following culturing method.
グルコースを炭素源としたｐＨ５〜７の培養液中に担子
菌を植種し、最初、培養液を強力に撹拌培養して菌糸を
分散させた後、次いでゆるやかな撹拌を行うことによ少
この分散した菌糸を核として増殖を行わせ、小さなペレ
ット状の担子菌群を多数形成させてやる。Basidiomycetes are inoculated into a culture solution with a pH of 5 to 7 using glucose as a carbon source, and the culture solution is first cultured with strong agitation to disperse the hyphae, followed by gentle agitation. The dispersed hyphae are used as nuclei to proliferate, forming a large number of small pellet-shaped basidiomycete groups.
本発明は上記の知見に基づいて完成したものであって、
フ゛ミン酸を分解する能力を有するマイロセシユーム属
、グ鑓イロトス属、カリオラス属の担子菌の増殖方法に
おいてまず、糖類を主炭素源とするｐＨ５〜７の培養液
で、担子菌を好気的条件下で強い撹拌培養を行って分散
増殖させた後、次いで弱い撹拌培養を行って分散した菌
糸を核としてペレット増殖させることを特徴とするフミ
ン酸を分解する微生物の増殖方法である。The present invention was completed based on the above findings, and
In the method for growing basidiomycetes of the genus Mylocesium, Guirotos, and Cariolus, which have the ability to decompose fiminic acid, the basidiomycetes are first grown under aerobic conditions in a culture solution with a pH of 5 to 7 that uses sugars as the main carbon source. This is a method for propagating microorganisms that decompose humic acid, which is characterized by carrying out strong agitation culture for dispersion propagation, followed by weak agitation culture and propagating the dispersed hyphae into pellets using the dispersed hyphae as nuclei.
本発明で云う強い撹拌培養とは、担子菌がばらばらに分
散する位の撹拌強度を培養液に与えて行う培養を、また
弱い撹拌培養とは、担子菌の１糸がばらばらにならず、
かたまりの状態で増殖する位の撹拌強度を培養液に与え
て行う培養を意味する。Strong agitation culture as used in the present invention refers to culture carried out by applying agitation intensity to the culture solution that is sufficient to disperse the basidiomycetes, and weak agitation culture refers to culture in which a single filament of the basidiomycetes does not become scattered.
This refers to culturing in which the agitation intensity is applied to the culture medium to the extent that it grows in clumps.
小さなペレット状の菌を多数形成することにより、菌の
沈降速度は速くなり、かつ、分散性も保持することがで
きる。By forming a large number of small pellet-shaped bacteria, the sedimentation rate of the bacteria becomes faster and dispersibility can be maintained.
小さなペレット状の担子菌群を培養液中に多数形成する
ための培養条件を見い出すため下記実験を行った。The following experiment was conducted to find culture conditions for forming a large number of small pellet-shaped basidiomycete groups in the culture solution.
〔実験例１〕
まず、担子菌を活発に増殖させるための培養液の最適−
値を見い出す実験を行った。グルコース：５１／Ｊ３、
Ｎ）Ｉ４（’／ ： ０．１．９ ／看、ＫＨ２ＰＯ４
：ｔ、０９ ／ １３ 、 Ｍｇ８０４・７Ｈ２０：
０．５９ ／看、酵母エキス： ｏ、Ｄ １９／１３の
組成の培養液２ＵＯＩｄずつを、容量５００ｄの坂ロフ
ラスコに入れ、それぞれ、ｐｉ（５，０，４，０，５，
０，６，０，７，０，８，０，９，０の７段階調整した
ものを準備する。[Experimental Example 1] First, the optimum culture solution for actively growing basidiomycetes.
We conducted an experiment to find the value. Glucose: 51/J3,
N)I4('/: 0.1.9 /KH2PO4
:t, 09/13, Mg804・7H20:
0.59/min, Yeast extract: o, D 2 UOId of culture solution with the composition of 19/13 was put into a 500 d capacity Sakalo flask, and each of them was added with pi (5, 0, 4, 0, 5,
Prepare 7-step adjustments: 0, 6, 0, 7, 0, 8, 0, 9, 0.
オートクレーブで１２１Ｃ１５分間殺菌後、担子菌マイ
ロセクユームを白金耳で培養液に少量植種後、３０ Ｇ
、 ９０ ｒ、ｐ、ｍで４８時間振とり培養を行い増
殖状況を観察した。その結果、−１５，０，６，０，７
，０の条件で担子菌は非常に良く増殖を行ったが…４．
０．８．０ではわずかじか増殖せず、ｐｉ（５，０，９
，０の条件では全く増殖が認められなかった。After sterilizing in an autoclave at 121C for 15 minutes, inoculate a small amount of basidiomycete Myrosequium into the culture medium using a platinum loop, and then inoculate at 30G.
, 90 r, p, m for 48 hours with shaking culture, and the growth status was observed. As a result, -15,0,6,0,7
, Basidiomycetes grew very well under conditions of 0, but...4.
0.8.0, there was little growth, pi(5,0,9
, 0 conditions, no growth was observed at all.
従ってこの担子菌の最適ｐ）４値は５．０〜７．０テあ
ることを確認した。Therefore, it was confirmed that the optimal p)4 value of this basidiomycete is 5.0 to 7.0.
〔実験例２〕
実験例１で使用した組成のグルコース培地をｐ）１５．
０〜７．０に調整し、ペレット形成条件を見い出す実験
を行った。具体的にはこのグルコース培地２００Ｒｊ′
！！−容ｉ５００ｍＡの坂ロフラスコに入れ、オートク
レーブ殺菌した後、担子菌マイロセシユーム属を一白金
耳植ｉ、３０ｔ？で下記条件にて好気的に振とり培養を
行った。[Experimental Example 2] The glucose medium having the composition used in Experimental Example 1 was prepared using p)15.
An experiment was conducted to find the conditions for forming pellets. Specifically, this glucose medium 200Rj'
! ! - After placing in a Sakalo flask with a capacity of 500 mA and sterilizing it in an autoclave, I planted a loop of basidiomycete Mylocesium and 30 tons. Shaking culture was performed aerobically under the following conditions.
条件１：振とう条件８Ｑ ｒ、ｐ、ｍで２４時間培培養
性２：振とり条件１００ ｒ、ｐ、ｍで２４時間培培養
性６：振とり条件１２０ ｒ、ｐ、ｍで２４時間培養条
件４：振とう条件１４０ ｒ、ｐ、ｍで２４時間培養条
件５ ： １４０ ｒ、ｐ、ｍで１０時間培養後、８０
ｒ、ｐ、ｍで１４時間培養
この結果、条件１のゆるやかな撹拌条件では培養液中に
１０〜２０關φの大きさに成長した担子菌の団塊が２〜
３個認められたにすぎなかった。また条件４の非常に強
く撹拌した条件では、菌糸はほとんど分散してしまい、
沈降性は極めて遅いものであった。ちなみにこの時の沈
降速度は０．１〜０．５ ｍ ／ Ｈｒ である。Condition 1: Culture culturability for 24 hours under shaking conditions 8Q r, p, m 2: Culture culturability for 24 hours under shaking conditions 100 r, p, m 6: Culture for 24 hours under shaking conditions 120 r, p, m Condition 4: Shaking condition 140 r, p, m for 24 hours Culture condition 5: After culturing for 10 hours at 140 r, p, m, 80
Cultured for 14 hours at R, P, and M. As a result, under the gentle stirring condition of condition 1, there were 2 to 2 basidiomycete nodules that grew to a size of 10 to 20 mm in the culture solution.
Only three were recognized. In addition, under condition 4, where the agitation is very strong, most of the mycelia are dispersed.
Sedimentation was extremely slow. Incidentally, the sedimentation rate at this time is 0.1 to 0.5 m/Hr.
次に条件２．５では色々な大きさの担子菌の団塊（０，
１〜１０龍φ）が生成し、沈降速度も数十ｃＩＩＬ／時
間〜数百ｍ／時間と幅がありばらつきが見られた。Next, under condition 2.5, basidiomycete clusters of various sizes (0,
1 to 10 dragons φ) were formed, and the sedimentation rate varied widely from several tens of cIIL/hour to several hundred m/hour.
条件５では最初の１０時間は非常に強い撹拌によシ菌糸
をばらばらにし、次の１４時間のゆるやかな撹拌でばら
ばらの状態の菌糸を核としてペレットを形成させようと
したものであるが、この結果的１〜２ｗａ１φの大きさ
のほば均一なペレットが培養液中に高密度（数十個／１
）で得ることができ次。ちなみに、この沈降速度は１０
〜５０ｍ／時間と相当速いものであり１条件４に比較し
て約１００倍も沈降速度を上げることができた。In condition 5, the mycelia were broken up by very strong stirring for the first 10 hours, and then the pellets were formed using the broken hyphae as cores by gentle stirring for the next 14 hours. As a result, almost uniform pellets with a size of 1 to 2 wa 1φ were placed in the culture medium at a high density (several tens of pellets/1
) can be obtained with the following. By the way, this sedimentation rate is 10
It was quite fast at ~50 m/hour, and the sedimentation speed could be increased about 100 times compared to 1 condition 4.
なお、フミン酸を分解できる担子菌は炭素源としてグル
コースをよく資化することがわかっており、これらの実
験でもグルコースを用いたが、その他、糖類の一つであ
るサッカロース、糖蜜なども同様の効果を得ることがで
きる。Note that basidiomycetes that can decompose humic acid are known to well utilize glucose as a carbon source, and glucose was used in these experiments, but other sugars such as saccharose and molasses can also be used in the same way. effect can be obtained.
本発明により、
■ 沈降速度が速い担子菌が得られ、沈殿槽がコンパク
ト化できる。According to the present invention, (1) a basidiomycete with a high sedimentation rate can be obtained, and a sedimentation tank can be made compact;
■ 小さなペレットを多数形成させることができるので
、フミン酸含有水との接触面積、接触効率が高くなシ分
解性能が向上する。■ Since many small pellets can be formed, the contact area with humic acid-containing water and the contact efficiency are high, which improves the silica decomposition performance.
■ 沈殿槽での担子菌の回収、反応槽への返送が容易と
なシ、反応槽での担子＠濃度を高く維持できることから
フミン酸分解速度、処理性能を上げることができる。■ Recovery of basidiomycetes in the sedimentation tank and return to the reaction tank are easy, and the basidiomycete concentration in the reaction tank can be maintained at a high level, thereby increasing the humic acid decomposition rate and treatment performance.
Claims (1)
プロイロトス属、カリオラス属の担子菌の増殖方法にお
いてまず、糖類を主炭素源とするｐＨ５〜７の培養液で
、担子菌を好気的条件下で強い撹拌培養を行つて分散増
殖させた後次いで弱い撹拌培養を行つて分散した菌糸を
核としてペレット増殖させることを特徴とするフミン酸
を分解する微生物の増殖方法。Mylocesium spp., which has the ability to degrade humic acid;
In the method for propagating basidiomycetes of the genus Proirotus and Cariolus, first, the basidiomycetes are cultured under aerobic conditions with strong agitation in a culture solution with a pH of 5 to 7 containing sugars as the main carbon source, and then the basidiomycetes are grown in a dispersed manner. A method for propagating microorganisms that decompose humic acid, which is characterized by performing weak agitation culture and propagating dispersed mycelia in pellets as nuclei.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63167830A JPH0218000A (en) | 1988-07-07 | 1988-07-07 | Propagating method of microorganism for decomposing humic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63167830A JPH0218000A (en) | 1988-07-07 | 1988-07-07 | Propagating method of microorganism for decomposing humic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0218000A true JPH0218000A (en) | 1990-01-22 |
Family
ID=15856881
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63167830A Pending JPH0218000A (en) | 1988-07-07 | 1988-07-07 | Propagating method of microorganism for decomposing humic acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0218000A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0775555A (en) * | 1993-09-08 | 1995-03-20 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | Culture of fibrous algae |
-
1988
- 1988-07-07 JP JP63167830A patent/JPH0218000A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0775555A (en) * | 1993-09-08 | 1995-03-20 | Chikyu Kankyo Sangyo Gijutsu Kenkyu Kiko | Culture of fibrous algae |
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