JPH0211231B2 - - Google Patents
Info
- Publication number
- JPH0211231B2 JPH0211231B2 JP27895686A JP27895686A JPH0211231B2 JP H0211231 B2 JPH0211231 B2 JP H0211231B2 JP 27895686 A JP27895686 A JP 27895686A JP 27895686 A JP27895686 A JP 27895686A JP H0211231 B2 JPH0211231 B2 JP H0211231B2
- Authority
- JP
- Japan
- Prior art keywords
- saccharification
- hours
- liquid
- potatoes
- certain amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 244000061456 Solanum tuberosum Species 0.000 claims description 14
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 14
- 235000007164 Oryza sativa Nutrition 0.000 claims description 13
- 235000009566 rice Nutrition 0.000 claims description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 12
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- 235000012015 potatoes Nutrition 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 240000006439 Aspergillus oryzae Species 0.000 claims description 4
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 4
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 3
- 102100022624 Glucoamylase Human genes 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 102000004139 alpha-Amylases Human genes 0.000 claims description 2
- 108090000637 alpha-Amylases Proteins 0.000 claims description 2
- 229940024171 alpha-amylase Drugs 0.000 claims description 2
- 235000010410 calcium alginate Nutrition 0.000 claims description 2
- 239000000648 calcium alginate Substances 0.000 claims description 2
- 229960002681 calcium alginate Drugs 0.000 claims description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 238000000855 fermentation Methods 0.000 description 13
- 230000004151 fermentation Effects 0.000 description 13
- 241000209094 Oryza Species 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 235000020054 awamori Nutrition 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 235000010413 sodium alginate Nutrition 0.000 description 2
- 239000000661 sodium alginate Substances 0.000 description 2
- 229940005550 sodium alginate Drugs 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 235000020083 shōchū Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000013555 soy sauce Nutrition 0.000 description 1
Landscapes
- Alcoholic Beverages (AREA)
Description
Γ産業上の利用分野
本発明は、河内白麹菌を用いた米麹の糖化ロ液
と無蒸煮液化糖化ジヤガイモの混合液を濃縮酵母
菌を含んだゲル型球状アルギン酸カルシウムの反
応槽に一定量連続的に注入し連続的にジヤガイモ
しようちゆうもろみを製造する方法で、本法をジ
ヤガイモしようちゆう製造に利用することによつ
て発酵期間の大幅な短縮および設備の簡素化、省
力化に大きく寄与できる。
Γ従来の技術
従来の連続的アルコール製造方法は主に燃料用
アルコール製造技術に関するもので、ジヤガイモ
しようちゆう(乙類)の連続的製造については、
いまだ行われていない。また、乙類しようちゆう
の製法は通常、米麹に発酵酵母を加えて6日間1
次発酵を行い、これに主原料である麦、いも等を
加えて10〜12日間2次発酵を行つてしようちゆう
もろみを製造しており、発酵期間が長く、発酵期
間の短縮および設備の省力化を図ることができな
いのが現状である。
Γ発明が解決しようとする問題点
現在、行われている乙類しようちゆうの製法は
仕込タンクに、米麹と発酵酵母を加えて発酵を行
い、1次もろみを製造し、さらに主原料を1次も
ろみに投入して一定期間発酵させ、2次もろみを
製造し、これを蒸留して、しようちゆうを製造し
ているのが通例である。
また、通常の蒸し法で仕込む方法は、香味にす
ぐれたジヤガイモしようちゆうを製造することが
できず、麦しようちゆうとブレンドして出荷して
いるのが現状である。
本発明により無蒸煮液化、糖化して得られたジ
ヤガイモ原料と米麹のロ液の混合液を定量ポンプ
で、一定量連続発酵装置に注入し、連続的に発酵
させ、連続的にしようちゆうもろみを製造するこ
とにより、発酵期間の短縮、設備の省力化を図る
ことができるとともに、酵素分解法により製造す
るので、マイルドで飲みやすいしようちゆうが製
造でき、工業上極めて有用な方法であると考えら
れる。
Γ問題点を解決するための手段
河内白麹菌を用いた米麹に加水して、55℃、24
時間米麹の糖化を行つた後ガーゼでロ過し、ロ液
を得る。
ジヤガイモをミキサーで紛砕し、これに液化酵
素であるα−アミラーゼを一定量加えて、80℃、
10分間液化を行い、さらに糖化酵素であるグルコ
アミラーゼを一定量加えて55℃、24時間糖化して
ジヤガイモ試料を得る。
別に2.5%アルギン酸ナトリウム溶液に24時間
振とう培養して得られた濃縮酵母菌株を入れて混
合した溶液を1.5%塩化カルシウム水溶液中に定
量ポンプを使用して一定速度で注入し、球状のゲ
ル型リアクター材を製造し、反応槽に一定量つめ
込む。球状のゲル型リアクターを充填した反応槽
に米麹の糖化ロ液と無蒸煮液化、糖化ジヤガイモ
の混合試料を定量ポンプを使用して一定速度で注
入し、発酵させて得られたしようちゆうもろみを
一定速度で取り出すことによつて連続的にしよう
ちゆうもろみを製造することができる。
Γ作用
米麹の糖化ロ液と無蒸煮液化、糖化ジヤガイモ
試料の混合試料を濃縮酵母菌を含有した球状ゲル
型反応体と接触することにより、効率よく、かつ
マイルドに飲みやすいしようちゆうを製造するこ
とができる。
Γ実施例
河内白麹菌を用いた米麹500gを三角フラスコ
に取り、これに水500mlを加えて55℃、24時間米
麹の糖化を行い、糖化液をガーゼでロ過してロ液
を得る。
ジヤガイモの皮をむき、これをミキサーで紛砕
し、1Kgをビーカーにとり、液化酵素であるα−
アミラーゼを1ml加えて80℃、10分間液化を行い
さらに糖化酵素であるグルコアミラーゼを一定量
加えて55℃、24時間糖化を行い、得られた糖化ジ
ヤガイモと糖化米麹ロ液を1対1の割合で混合し
て試料とする。
別に発酵酵母菌(鹿児島工試酵母、泡盛酵母)
を入れたシード培養液を30℃、24時間振とう培養
し、遠心分離を行つて得られた濃縮酵母菌株を
2.5%アルギン酸ナトリウム水溶液に入れ、これ
を1.5%塩化カルシウム水溶液中に定量ポンプを
使用して一定速度で注入し、球状のゲル型リアク
ター材を製造し、500mlの容器に250ml充填する。
発酵反応槽に糖化米麹ロ液と無蒸煮液化糖化ジ
ヤガイモの混合液を定量ポンプを使用して一定速
度で注入し、発酵中に生成する炭酸ガス量の測
定、発酵もろみのPH、残糖、アルコール度数お
よび蒸留して得られたしようちゆうの香味につい
て検討を行つた。その図1,2、表1、2に示
す。
図1,2の結果より良好に発酵していることが
認められる。表1より発酵もろみのPH、残糖、
および生成アルコール度数を測定した結果、発酵
もろみの残糖もD=1.8day1、D=3.6day1の範囲
において1%前後であり、しようちゆうの香味の
点から考えても、蒸留したしようちゆうに適度の
甘さが残り良好なしようちゆうもろみであること
が認められ、また、発酵歩合についても90%前後
と良好に発酵していることがわかつた。
さらに蒸留して得られたしようちゆうの香味に
ついて検討を行つた結果、しようちゆうもろみに
適度の残糖が在つた方が蒸留して得られたしよう
ちゆうに適度の甘さと香りが残り、良好なしよう
ちゆうが製造できることが認められた。
以上の結果より、本発明はしようちゆうもろみ
製造の省力化に大きく寄与でき、きわめて重要な
発明であると考えられる。
ΓIndustrial Application Field The present invention is a method of continuously supplying a fixed amount of a mixed solution of rice koji saccharification using Kawachi white koji mold and non-cooked liquefied saccharified potatoes to a reaction tank of gel-type spherical calcium alginate containing concentrated yeast. This is a method of continuously producing potato yochi yuu mash by injecting the yam and yam mash, and by using this method in the production of yam yochi yuu, it greatly shortens the fermentation period, simplifies equipment, and saves labor. can. ΓConventional technology The conventional continuous alcohol production method is mainly related to fuel alcohol production technology, and regarding the continuous production of ginger (Type O),
It has not been done yet. In addition, the manufacturing method for Otsu-rui Shochiyu is usually made by adding fermented yeast to rice malt for 6 days.
After the second fermentation, the main raw materials such as barley and potatoes are added and the second fermentation is carried out for 10 to 12 days to produce shōchiyu moromi. The current situation is that it is not possible to save labor. Problems that the Γ invention aims to solve The currently used manufacturing method for Otsu-type shochiyu involves adding rice malt and fermented yeast to a brewing tank, fermenting it, producing primary mash, and then adding the main raw materials to 1 mash. It is customary to add it to the next mash and ferment it for a certain period of time to produce a secondary mash, which is then distilled to produce shochiyu. In addition, the usual steaming method cannot produce potato shochiyu with excellent flavor, so it is currently shipped after being blended with barley shochiyu. Using a metering pump, a fixed amount of the mixed liquid of the raw potato raw material obtained by liquefying and saccharifying rice without steaming and saccharification according to the present invention is injected into a continuous fermentation device, where it is continuously fermented and fermented continuously. By producing moromi, it is possible to shorten the fermentation period and save labor on equipment, and since it is produced using an enzymatic decomposition method, it is possible to produce mild and easy-to-drink shochiyu, which is an extremely useful method industrially. it is conceivable that. Measures to solve the Γ problem Add water to rice malt using Kawachi white koji mold and heat at 55℃ for 24 hours.
After saccharifying the rice malt for an hour, it is filtered through gauze to obtain a filtered liquid. Crush potatoes with a mixer, add a certain amount of α-amylase, a liquefying enzyme, and heat at 80℃.
Liquefy for 10 minutes, then add a certain amount of glucoamylase, a saccharifying enzyme, and saccharify at 55°C for 24 hours to obtain a potato sample. Separately, a concentrated yeast strain obtained by shaking culture for 24 hours was added to a 2.5% sodium alginate solution, and the mixed solution was injected into a 1.5% calcium chloride aqueous solution at a constant rate using a metering pump to form a spherical gel. Manufacture reactor materials and fill a certain amount into a reaction tank. A mixed sample of rice malt saccharification filtrate, non-simmering liquefaction, and saccharified potatoes was injected at a constant rate into a reaction tank filled with a spherical gel-type reactor using a metering pump, and the mixture was fermented. By taking out the mash at a constant rate, it is possible to continuously produce mash. Γ action By contacting a mixed sample of rice koji saccharification liquid, non-cooked liquefaction, and saccharified potato sample with a spherical gel-type reactant containing concentrated yeast, we efficiently produce mild and easy-to-drink shochiyu. can do. ΓExample: Take 500g of rice malt using Kawachi white koji mold into an Erlenmeyer flask, add 500ml of water to it, saccharify the rice malt at 55℃ for 24 hours, and filter the saccharified liquid through gauze to obtain a filtrate. . Peel the potato, crush it with a mixer, put 1 kg in a beaker, and add α-, which is a liquefying enzyme.
Add 1 ml of amylase and liquefy at 80℃ for 10 minutes, then add a certain amount of glucoamylase, a saccharifying enzyme, and saccharify at 55℃ for 24 hours. Mix in proportion and use as a sample. Separate fermentation yeast (Kagoshima Kogyo yeast, Awamori yeast)
The concentrated yeast strain obtained by culturing the seed culture with shaking at 30℃ for 24 hours and centrifuging
Put it in a 2.5% sodium alginate aqueous solution and inject it into a 1.5% calcium chloride aqueous solution at a constant rate using a metering pump to produce a spherical gel type reactor material, and fill 250 ml into a 500 ml container. A mixture of saccharified rice malt liquid and non-cooked liquefied saccharified potatoes was injected into the fermentation reaction tank at a constant rate using a metering pump, and the amount of carbon dioxide produced during fermentation was measured, the pH of the fermented mash, residual sugar, We investigated the alcohol content and the flavor of the distilled soy sauce. It is shown in Figures 1 and 2 and Tables 1 and 2. From the results shown in Figures 1 and 2, it can be seen that the fermentation was good. From Table 1, the pH of fermented mash, residual sugar,
And as a result of measuring the alcohol content produced, the residual sugar in the fermented mash was around 1% in the range of D = 1.8day 1 and D = 3.6day 1. Considering the flavor of shochiyu, it was found that the residual sugar of fermented mash was around 1%. It was found that the sake mash was of good quality with just the right amount of sweetness remaining, and the fermentation rate was also found to be around 90%, indicating good fermentation. Furthermore, as a result of studying the flavor of the distilled shochiyu, we found that if the shochiyu moromi had an appropriate amount of residual sugar, the distilled shochiyu would retain an appropriate amount of sweetness and aroma. It was confirmed that a good product could be produced. From the above results, it is considered that the present invention can greatly contribute to labor saving in the production of shochu mash and is an extremely important invention.
【表】【table】
【表】【table】
【表】【table】
図1は鹿児島工試酵母を使用した連続発酵過程
を示す説明図である。図2は泡盛酵母を使用した
連続発酵過程を示す図である。
FIG. 1 is an explanatory diagram showing a continuous fermentation process using Kagoshima Kogyo yeast. FIG. 2 is a diagram showing a continuous fermentation process using awamori yeast.
Claims (1)
時間糖化を行い、得られた糖化液をカーゼでロ過
し、ロ液を得る。さらにジヤガイモをミキサーで
粉砕し、これに一定量の液化酵素であるα−アミ
ラーゼを加えて、液化を行つた後に一定量の糖化
酵素であるグルコアミラーゼを加えて55℃、24時
間糖化を行い、得られたジヤガイモの糖化液と米
麹の糖化ロ液を一定割合に混合し、これを、濃縮
酵母菌株を固定したゲル型球状アルギン酸カルシ
ウム反応槽に一定速度で通液し、連続的にジヤガ
イモしようちゆうもろみを製造する方法。1. Add water to rice malt using Kawachi white koji mold and heat at 55℃ for 24 hours.
Saccharification is performed for a period of time, and the resulting saccharified liquid is filtered through a case to obtain a filtrate. Furthermore, the potatoes are crushed with a mixer, a certain amount of α-amylase, which is a liquefaction enzyme, is added to this, and after liquefaction, a certain amount of glucoamylase, which is a saccharification enzyme, is added to perform saccharification at 55℃ for 24 hours. The obtained potato saccharification liquid and rice malt saccharification liquid are mixed at a constant ratio, and this is passed at a constant rate through a gel-type spherical calcium alginate reaction tank in which a concentrated yeast strain is immobilized to continuously grow potatoes. How to make Chiyu Moromi.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61278956A JPS63133976A (en) | 1986-11-25 | 1986-11-25 | Continuous production of unrefined sake of potato 'shochu' (low-class distilled spirit) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP61278956A JPS63133976A (en) | 1986-11-25 | 1986-11-25 | Continuous production of unrefined sake of potato 'shochu' (low-class distilled spirit) |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS63133976A JPS63133976A (en) | 1988-06-06 |
JPH0211231B2 true JPH0211231B2 (en) | 1990-03-13 |
Family
ID=17604404
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP61278956A Granted JPS63133976A (en) | 1986-11-25 | 1986-11-25 | Continuous production of unrefined sake of potato 'shochu' (low-class distilled spirit) |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS63133976A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100509165B1 (en) * | 2002-12-03 | 2005-08-22 | 주식회사 두산 | Fermented liquor using purified enzyme and dried koji and preparation method thereof |
-
1986
- 1986-11-25 JP JP61278956A patent/JPS63133976A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS63133976A (en) | 1988-06-06 |
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