JPH0146111B2 - - Google Patents
Info
- Publication number
- JPH0146111B2 JPH0146111B2 JP3446781A JP3446781A JPH0146111B2 JP H0146111 B2 JPH0146111 B2 JP H0146111B2 JP 3446781 A JP3446781 A JP 3446781A JP 3446781 A JP3446781 A JP 3446781A JP H0146111 B2 JPH0146111 B2 JP H0146111B2
- Authority
- JP
- Japan
- Prior art keywords
- gene
- protein
- lactamase
- sequence
- plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000623 proteins and genes Proteins 0.000 claims description 89
- 239000013612 plasmid Substances 0.000 claims description 57
- 102000004169 proteins and genes Human genes 0.000 claims description 41
- 108090000204 Dipeptidase 1 Proteins 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 31
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 30
- 102000006635 beta-lactamase Human genes 0.000 claims description 23
- 239000013599 cloning vector Substances 0.000 claims description 11
- 108091008146 restriction endonucleases Proteins 0.000 claims description 9
- 239000013598 vector Substances 0.000 claims description 9
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical group N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 8
- 230000010076 replication Effects 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 230000027455 binding Effects 0.000 claims description 7
- 102000004877 Insulin Human genes 0.000 claims description 6
- 108090001061 Insulin Proteins 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 230000014509 gene expression Effects 0.000 claims description 4
- 229940125396 insulin Drugs 0.000 claims description 4
- 241000192125 Firmicutes Species 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims 1
- 239000005556 hormone Substances 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 230000007723 transport mechanism Effects 0.000 claims 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 44
- 108020004414 DNA Proteins 0.000 description 39
- 239000002773 nucleotide Substances 0.000 description 34
- 125000003729 nucleotide group Chemical group 0.000 description 34
- 235000018102 proteins Nutrition 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 29
- 244000063299 Bacillus subtilis Species 0.000 description 27
- 241000588724 Escherichia coli Species 0.000 description 24
- 241000194108 Bacillus licheniformis Species 0.000 description 23
- 239000012634 fragment Substances 0.000 description 23
- 150000001413 amino acids Chemical group 0.000 description 17
- 230000032258 transport Effects 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 12
- 244000005700 microbiome Species 0.000 description 12
- 210000000170 cell membrane Anatomy 0.000 description 11
- 238000010586 diagram Methods 0.000 description 10
- 238000003556 assay Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 108020004511 Recombinant DNA Proteins 0.000 description 7
- 229960000723 ampicillin Drugs 0.000 description 7
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 230000001588 bifunctional effect Effects 0.000 description 6
- 102000037865 fusion proteins Human genes 0.000 description 6
- 108020001507 fusion proteins Proteins 0.000 description 6
- 230000028327 secretion Effects 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 101150084044 P gene Proteins 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 3
- 238000012215 gene cloning Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 210000001322 periplasm Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 108020004256 Beta-lactamase Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 101000695691 Bacillus licheniformis Beta-lactamase Proteins 0.000 description 1
- 101100498939 Bacillus subtilis (strain 168) degS gene Proteins 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 102100029018 Gametogenetin Human genes 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 108010036940 Levansucrase Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 description 1
- 108010066154 Nuclear Export Signals Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- DXYQIGZZWYBXSD-JSGCOSHPSA-N Trp-Pro Chemical compound O=C([C@H](CC=1C2=CC=CC=C2NC=1)N)N1CCC[C@H]1C(O)=O DXYQIGZZWYBXSD-JSGCOSHPSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000008260 defense mechanism Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- KZNQNBZMBZJQJO-YFKPBYRVSA-N glyclproline Chemical compound NCC(=O)N1CCC[C@H]1C(O)=O KZNQNBZMBZJQJO-YFKPBYRVSA-N 0.000 description 1
- 108010077515 glycylproline Proteins 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 101150079396 trpC2 gene Proteins 0.000 description 1
- 108010084932 tryptophyl-proline Proteins 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12853780A | 1980-03-10 | 1980-03-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56137896A JPS56137896A (en) | 1981-10-28 |
| JPH0146111B2 true JPH0146111B2 (enrdf_load_stackoverflow) | 1989-10-05 |
Family
ID=22435806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3446781A Granted JPS56137896A (en) | 1980-03-10 | 1981-03-10 | Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56137896A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11698642B2 (en) | 2017-12-12 | 2023-07-11 | Sony Corporation | Information processing apparatus, mobile object, control system, and information processing method |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| NZ208806A (en) * | 1983-07-06 | 1988-07-28 | Gist Brocades Nv | Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids |
| JPS6091980A (ja) * | 1983-07-06 | 1985-05-23 | ジェネックス・コーポレイション | プロテア−ゼ酵素の発現のための徴生物を製造する方法 |
| JPS6030687A (ja) * | 1983-08-01 | 1985-02-16 | Wakunaga Seiyaku Kk | Ｄνａ遺伝子，その製造法およびそれを含むプラスミド |
| JPS60188093A (ja) * | 1984-03-09 | 1985-09-25 | Teruhiko Beppu | 枯草菌に於ける形質発現プラスミド |
| JPS6112286A (ja) * | 1984-06-27 | 1986-01-20 | Ajinomoto Co Inc | プラスミドベクタ− |
| JP2524693B2 (ja) * | 1984-07-30 | 1996-08-14 | 湧永製薬株式会社 | 蛋白質の製造法 |
| JP2524695B2 (ja) * | 1984-12-26 | 1996-08-14 | 湧永製薬株式会社 | 蛋白質の製造法 |
-
1981
- 1981-03-10 JP JP3446781A patent/JPS56137896A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11698642B2 (en) | 2017-12-12 | 2023-07-11 | Sony Corporation | Information processing apparatus, mobile object, control system, and information processing method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56137896A (en) | 1981-10-28 |
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