JPH0129761B2 - - Google Patents
Info
- Publication number
- JPH0129761B2 JPH0129761B2 JP21768686A JP21768686A JPH0129761B2 JP H0129761 B2 JPH0129761 B2 JP H0129761B2 JP 21768686 A JP21768686 A JP 21768686A JP 21768686 A JP21768686 A JP 21768686A JP H0129761 B2 JPH0129761 B2 JP H0129761B2
- Authority
- JP
- Japan
- Prior art keywords
- sample holder
- cells
- frozen
- tube
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000007710 freezing Methods 0.000 claims description 18
- 230000008014 freezing Effects 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 18
- 239000001307 helium Substances 0.000 claims description 12
- 229910052734 helium Inorganic materials 0.000 claims description 12
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000005871 repellent Substances 0.000 claims description 3
- 239000004642 Polyimide Substances 0.000 claims description 2
- 229920001721 polyimide Polymers 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- -1 polytetrafluoroethylene Polymers 0.000 claims 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims 1
- 239000004810 polytetrafluoroethylene Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 62
- 238000001816 cooling Methods 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 230000004083 survival effect Effects 0.000 description 8
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 5
- 238000010583 slow cooling Methods 0.000 description 4
- 239000002577 cryoprotective agent Substances 0.000 description 3
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 239000007798 antifreeze agent Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D2400/00—General features of, or devices for refrigerators, cold rooms, ice-boxes, or for cooling or freezing apparatus not covered by any other subclass
- F25D2400/28—Quick cooling
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21768686A JPS6372601A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21768686A JPS6372601A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6372601A JPS6372601A (ja) | 1988-04-02 |
| JPH0129761B2 true JPH0129761B2 (zh) | 1989-06-14 |
Family
ID=16708129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21768686A Granted JPS6372601A (ja) | 1986-09-16 | 1986-09-16 | 生体細胞の凍結装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6372601A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08304242A (ja) * | 1995-05-15 | 1996-11-22 | Rigaku Corp | 試料冷却ノズル |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10237125A1 (de) * | 2002-08-13 | 2004-03-11 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Kryokonservierung mit einem gas- oder dampfförmigen Kühlmedium |
| JP5261496B2 (ja) * | 2007-11-20 | 2013-08-14 | マックス プランク ゲゼルシャフト ツゥアー フェデルゥン デル ヴィッセンシャフテン エー フォー | 超急速冷凍装置及び超急速冷凍方法 |
| US8168138B2 (en) * | 2010-12-22 | 2012-05-01 | Li Che | Cryogenic vial |
| CN104986426B (zh) * | 2015-06-10 | 2017-07-07 | 陈子江 | 一种样品冷冻储存管及冷冻储存装置 |
-
1986
- 1986-09-16 JP JP21768686A patent/JPS6372601A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08304242A (ja) * | 1995-05-15 | 1996-11-22 | Rigaku Corp | 試料冷却ノズル |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6372601A (ja) | 1988-04-02 |
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