JPH01265224A - Cleaner for contact lens - Google Patents
Cleaner for contact lensInfo
- Publication number
- JPH01265224A JPH01265224A JP9377488A JP9377488A JPH01265224A JP H01265224 A JPH01265224 A JP H01265224A JP 9377488 A JP9377488 A JP 9377488A JP 9377488 A JP9377488 A JP 9377488A JP H01265224 A JPH01265224 A JP H01265224A
- Authority
- JP
- Japan
- Prior art keywords
- mold
- lens
- contact lenses
- soft contact
- cleaning agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 claims abstract description 21
- 108090000790 Enzymes Proteins 0.000 claims abstract description 21
- 108010022172 Chitinases Proteins 0.000 claims abstract description 15
- 102000012286 Chitinases Human genes 0.000 claims abstract description 14
- 108010089807 chitosanase Proteins 0.000 claims abstract description 14
- 101000763602 Manilkara zapota Thaumatin-like protein 1 Proteins 0.000 claims abstract description 9
- 101000763586 Manilkara zapota Thaumatin-like protein 1a Proteins 0.000 claims abstract description 9
- 101000966653 Musa acuminata Glucan endo-1,3-beta-glucosidase Proteins 0.000 claims abstract description 9
- 239000012459 cleaning agent Substances 0.000 claims description 26
- 230000000694 effects Effects 0.000 abstract description 14
- 210000002421 cell wall Anatomy 0.000 abstract description 12
- 229920001661 Chitosan Polymers 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 6
- 229920003002 synthetic resin Polymers 0.000 abstract description 4
- 239000000057 synthetic resin Substances 0.000 abstract description 4
- 229920002498 Beta-glucan Polymers 0.000 abstract description 2
- 108091005804 Peptidases Proteins 0.000 description 8
- 229920002101 Chitin Polymers 0.000 description 6
- 229920001503 Glucan Polymers 0.000 description 5
- 239000004365 Protease Substances 0.000 description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 241000228245 Aspergillus niger Species 0.000 description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000035859 Drug effect increased Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 102000040739 Secretory proteins Human genes 0.000 description 1
- 108091058545 Secretory proteins Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- PYMYPHUHKUWMLA-VPENINKCSA-N aldehydo-D-xylose Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-VPENINKCSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明はコンタクトレンズ用の洗浄剤、さらに詳しくは
、いわゆるソフトコンタクトレンズと称される合成樹脂
製のコンタクトレンズに付着する黴や細菌、或いはこれ
らの細胞壁を洗浄して除去するための洗浄剤に関する。Detailed Description of the Invention (Industrial Application Field) The present invention relates to a cleaning agent for contact lenses, and more specifically, to cleaning agents for cleaning molds and bacteria that adhere to synthetic resin contact lenses called so-called soft contact lenses. This invention relates to a cleaning agent for cleaning and removing these cell walls.
（従来の技術）
−Ｃに、コンタクトレンズにはガラス製のハードコンタ
クトレンズと、ハイドロキシエチルメタクリレート等の
合成樹脂からなる合成樹脂製のソフトコンタクトレンズ
とがあるが、後者のコンタクトレンズには黴や細菌が付
着し易いという固有の問題点があった。(Prior art) -C: Contact lenses include hard contact lenses made of glass and soft contact lenses made of synthetic resin made of synthetic resin such as hydroxyethyl methacrylate. There was an inherent problem that bacteria easily adhered to it.
すなわち、ソフトコンタクトレンズの場合、保水性でキ
ズが付き易く、涙中の分泌物（たとえば蛋白質等）がレ
ンズの表面に付着し、黴がソフトコンタクトレンズの隙
間に入って増殖し易い。そして、−度増殖した黴はレン
ズに根づき、除去するのは困難であり、これが原因で失
明する場合もごくまれにある。That is, soft contact lenses tend to be easily scratched due to their water-retaining properties, secretions from tears (eg, proteins, etc.) adhere to the surface of the lenses, and mold easily enters the gaps in the soft contact lenses and grows. The mold, which has multiplied to a certain degree, takes root in the lens and is difficult to remove, and in very rare cases, this can cause blindness.
そこで、このような問題点を解決するために、従来では
、蛋白質分解酵素であるプロテアーゼを含有したソフト
コンタクトレンズ専用の洗浄剤も開発されている。すな
わち、このプロテアーゼが涙分泌性蛋白質等を分解して
ソフトコンタクトレンズから除去するのである。In order to solve these problems, cleaning agents exclusively for soft contact lenses have been developed that contain protease, which is a proteolytic enzyme. That is, this protease decomposes lacrimal secretory proteins and the like and removes them from the soft contact lens.
（発明が解決しようとする問題点）
しかしながら、上記のような従来の洗浄剤は、あくまで
も分泌物（蛋白質等）を除去して黴等の生育し易い条件
を緩和するだけの機能しか有しないものであるため、黴
の発生を完全に防止することはできない。特に、−旦黴
が発生すると、その発生した黴自体を除去する能力はか
なり低いものであった。(Problems to be Solved by the Invention) However, the conventional cleaning agents described above only have the function of removing secretions (proteins, etc.) and easing the conditions that make it easy for mold to grow. Therefore, it is not possible to completely prevent the occurrence of mold. In particular, when -tan mold occurs, the ability to remove the generated mold itself is quite low.
本発明は、このような問題点を解決するためになされた
もので、黴の発生を略完全に防止し、仮に黴が発生して
も、その発生した黴を容易且つ確実に除去することので
きるコンタクトレンズ用の洗浄剤を提供することを目的
とする。The present invention was made to solve these problems, and it is possible to almost completely prevent the generation of mold, and even if mold does occur, it can be easily and reliably removed. The purpose of the present invention is to provide a cleaning agent for contact lenses that can be used as a cleaning agent.
（問題点を解決するための手段）
本発明は、このような目的を達成するために、上記ソフ
トコンタクトレンズに発生する黴を酵素で分解すること
によって黴を略完全に除去して上記問題点を解決せんと
するものである。(Means for Solving the Problems) In order to achieve the above object, the present invention solves the above problems by using enzymes to decompose the molds generated on the soft contact lenses to almost completely remove the molds. The aim is to solve this problem.
すなわち、本発明の上記問題点を解決するための手段は
、キチナーゼ、キトサナーゼ、又はβ−１，３−グルカ
ナーゼのうち、少なくとも１つの酵素を洗浄剤中に含有
したことにある。That is, a means for solving the above-mentioned problems of the present invention lies in containing at least one enzyme among chitinase, chitosanase, or β-1,3-glucanase in the detergent.
（作用）
そして、上記ソフトコンタクトレンズに発生し易い黴の
細胞壁には、一般にキチンやキトサン或いはβ−１，３
−グルカンが含有されているが、上記洗浄剤には上記の
ような酵素が含有されてなるため、その洗浄剤でソフト
コンタクトレンズを洗浄すると、洗浄剤に含有されてい
るキチナーゼ、キトサナーゼ、又はβ−１，３−グルカ
ナーゼのうち、少な（とも１つの酵素が、上記黴の細胞
壁に含有されているキチンやキトサン或いはβ−１，３
−グルカンのいずれかを分解し、それによって細胞壁が
分解されてソフトコンタクトレンズに付着した黴が除去
されることとなるのである。(Effect) The cell walls of mold that tend to grow on soft contact lenses are generally made of chitin, chitosan, or β-1,3.
-Although the cleaning agent contains glucan, the cleaning agent also contains the enzymes mentioned above, so when cleaning soft contact lenses with the cleaning agent, chitinase, chitosanase, or β contained in the cleaning agent Among the -1,3-glucanases, a small amount (at least one enzyme) contains chitin, chitosan, or β-1,3-glucanases contained in the cell wall of the mold.
- It decomposes some of the glucans, thereby degrading the cell walls and removing mold attached to soft contact lenses.
（実施例） 以下、本発明の実施例について説明する。(Example) Examples of the present invention will be described below.
夾胤阻土
本実施例におけるコンタクトレンズ用の洗浄剤は、ｐＨ
６，８、０，０１モルのリン酸カリウム水溶液中に、キ
チナーゼ、キトサナーゼ、β−１，３−グルカナーゼ、
及び蛋白質分解酵素が含有されて調製された液状のもの
である。The cleaning agent for contact lenses in this example has a pH of
Chitinase, chitosanase, β-1,3-glucanase,
It is a liquid prepared by containing and proteolytic enzyme.
そして、このような組成からなるコンタクトレンズ用の
洗浄剤を使用する場合につい６て説明すると、先ずその
洗浄剤の使用前に予めソフトコンタクトレンズに人為的
に黴を付着させる。When using a contact lens cleaning agent having such a composition, mold is first artificially attached to the soft contact lens before using the cleaning agent.
すなわち、使い古したソフトコンタクトレンズをポリペ
プトンと酵母エキスとを含有する生理食塩水に浸漬して
十分に蛋白質と水分を吸着させ、その裏面に黴の一種で
あるＡｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒ （［ＦＯ４
０６６）を塗布し、密閉容器に入れて３７°Ｃで培養し
、黴を十分に生育させる。That is, a worn-out soft contact lens is immersed in physiological saline containing polypeptone and yeast extract to sufficiently absorb protein and water, and the back side is coated with Aspergillus niger ([FO4
066) and cultured at 37°C in a sealed container to allow the mold to grow sufficiently.
次に、その黴の生育したソフトコンタクトレンズを１０
０°Ｃで５分間煮沸し、上記組成からなる洗浄剤中に室
温で約５〜６時間浸漬する。Next, remove the moldy soft contact lenses for 10 minutes.
It is boiled at 0°C for 5 minutes and immersed in a cleaning agent having the above composition at room temperature for about 5-6 hours.
その後、浸漬後のソフトコンタクトレンズを蒸留水で洗
浄し、顕微鏡で観察したところ上記黴が略完全に除去さ
れていることが確認できた。Thereafter, the soaked soft contact lenses were washed with distilled water, and when observed under a microscope, it was confirmed that the mold had been almost completely removed.
夫癒桝１
本実施例は、洗浄剤中の酵素として、キチナーゼ、β−
１，３−グルカナーゼ１及び蛋白質分解酵素を含有させ
た実施例である。その他の条件、すなわち溶剤や操作手
順等については上記実施例１と同様である。In this example, chitinase, β-
This is an example containing 1,3-glucanase 1 and a protease. Other conditions, such as the solvent and operating procedures, are the same as in Example 1 above.
実省Ｉ津よ
本実施例は、洗浄剤中の酵素として、キチナーゼ、キト
サナーゼ、及びβ−１，３−グルカナーゼを含有させた
実施例である。その他の条件は上記実施例１と同様であ
る。This example is an example in which chitinase, chitosanase, and β-1,3-glucanase were contained as enzymes in the cleaning agent. Other conditions were the same as in Example 1 above.
１施■１
本実施例は、洗浄剤中の酵素として、キチナーゼ及びβ
−１，３−グルカナーゼを含有させた実施例である。そ
の他の条件は上記実施例１と同様である。1.1 This example uses chitinase and β as enzymes in the cleaning agent.
This is an example containing -1,3-glucanase. Other conditions were the same as in Example 1 above.
ス遣更五
本実施例は、洗浄剤中の酵素として、β−１，３−グル
カナーゼのみを含有させた実施例である。This example is an example in which only β-1,3-glucanase was contained as the enzyme in the cleaning agent.
その他の条件は上記実施例１と同様である。Other conditions were the same as in Example 1 above.
夫施健１
本実施例は、洗浄剤中の酵素として、キチナーゼのみを
含有させた実施例である。その他の条件は上記実施例１
と同様である。Ken Husei 1 This example is an example in which only chitinase was contained as an enzyme in the cleaning agent. Other conditions are the above Example 1
It is similar to
実施桝工
本実施例は、洗浄剤中の酵素として、キトサナ−ゼのみ
を含有させた実施例である。その他の条件は上記実施例
１と同様である。EXAMPLE 1 This example is an example in which only chitosanase was contained as the enzyme in the cleaning agent. Other conditions were the same as in Example 1 above.
以上のような実施例１〜７について黴の除去効果につい
て確認したところ、次のような順位となった。When the mold removal effects of Examples 1 to 7 as described above were confirmed, the following rankings were obtained.
ｌ≧２〉３≧４ ＞５ ＞６ ＞７ ＞比較例向、それ
ぞれの数字は各実施例の数字を意味する。又、比較例と
は、酵素を含まない溶剤のみの液で上記と同様の操作で
黴を除去した場合についての結果である。l≧2>3≧4>5>6>7>Comparative example, each number means the number of each example. In addition, the comparative example is the result of removing mold using the same procedure as above using a solution containing only a solvent and no enzyme.
上記の結果からも明らかなように、溶剤中に含有される
酵素の種類の多いもの程、効果が強いことが認められた
。これは、細胞壁中にキチン、キトサン、β−１，３−
グルカン等の複数の成分が含まれているため、酵素も複
数使用されればその分効果が増大することが裏付けられ
るのである。As is clear from the above results, it was found that the more types of enzymes contained in the solvent, the stronger the effect. This contains chitin, chitosan, β-1,3-
Since it contains multiple components such as glucan, it is confirmed that the effect will increase if multiple enzymes are used.
又、蛋白質分解酵素を含有させれば、その分効果が増大
した。これは、細胞壁中に若干ながらも蛋白質が含有さ
れているためと推測される。Furthermore, when a proteolytic enzyme was included, the effect increased accordingly. This is presumed to be due to the presence of a small amount of protein in the cell wall.
さらに、上記結果においては、キトサナーゼの効果は弱
いものであった。これは、上記実施例に用いられた黴で
あるＡｓｐｅｒｇｉｌｌｕｓ ｎｉｇｅｒの細胞壁中の
キトサンの含有量が少ないためであり、たとえばキトサ
ンを大量に含む他の黴を使用すれば効果は増大すると認
められる。Furthermore, in the above results, the effect of chitosanase was weak. This is because the chitosan content in the cell wall of Aspergillus niger, which is the mold used in the above examples, is low, and it is recognized that the effect will be increased if, for example, another mold containing a large amount of chitosan is used.
尚、上記各実施例においては、黴としてＡｓｐｅｒｇｉ
ｌｌｕｓ ｎｉｇｅｒを使用したが、使用する黴の種類
はこれに限られるものではない。すなわち、上記ソフト
コンタクトレンズに発生し易い黴としては、上記Ａｓｐ
ｅｒｇｉｌｌｕｓ ｎｉｇｅｒの他、一般にＡｓｐｅｒ
ｇｔｌｌｕｓ ｆｕｍｉｇａｔｕｓ、Ａｓｐｅｒｇｉｌ
ｌｕｓ ｆｌａｖｕｓ、 Ｃａｎｄｉｄａ ｐａｒａｐ
ａｉｌｏｓｉｓ、 Ｃａｎｄｉｄａ ａｌｂｉｃａｎｓ
等のものが知られているが、このような黴や菌はいずれ
もその細胞壁にキチンやキトサン或いはβ−１，３−グ
ルカン等を含有しているため、上記各実施例のような酵
素を含有する洗浄剤によって黴や細菌の除去効果が認め
られるのである。ただし、対象となる黴等はこれらに限
定されるものではない。In addition, in each of the above examples, Aspergi was used as the mold.
llus niger was used, but the type of mold to be used is not limited to this. In other words, the mold that tends to occur on the soft contact lenses is Asp.
In addition to ergillus niger, Asper
gtllus fumigatus, Aspergillus
Candida parap
ailosis, Candida albicans
However, since all of these molds and bacteria contain chitin, chitosan, β-1,3-glucan, etc. in their cell walls, it is difficult to use enzymes such as those in the above examples. The cleaning agent it contains has been shown to be effective in removing mold and bacteria. However, the target molds, etc. are not limited to these.
又、上記実施例１や実施例２からも明らかなように、蛋
白質分解酵素の含有によって効果の増大が認められたが
、蛋白質分解酵素を含有させることは本発明においては
条件ではない。Furthermore, as is clear from Examples 1 and 2, the effect was increased by the inclusion of a protease, but the inclusion of a protease is not a condition for the present invention.
さらに、上記各実施例からも明らかなように、酵素とし
ては、キチナーゼ、キトサナーゼ、β−１，３−グルカ
ナーゼがすべて含有されている必要は必ずしもなく、黴
の細胞壁に含有されているキチン、キトサン、β−１，
３−グルカンのうち、少なくともいずれかが分解されれ
ば黴自体も分解され、コンタクトレンズからの除去には
十分である。Furthermore, as is clear from the above examples, the enzymes do not necessarily need to contain all of chitinase, chitosanase, and β-1,3-glucanase; chitin and chitosan, which are contained in the cell walls of mold, are not necessarily included. , β-1,
If at least one of the 3-glucans is decomposed, the mold itself is also decomposed, which is sufficient for removal from contact lenses.
従って、洗浄剤にはキチナーゼ、キトサナーゼ、β−Ｌ
３−グルカナーゼのうちの少なくとも１つの酵素が含
有されていればよいのである。Therefore, cleaning agents include chitinase, chitosanase, β-L
It is sufficient that it contains at least one enzyme among 3-glucanases.
尚、キチナーゼやキトサナーゼとしては、たとえばスト
レプトマイセス属その他のどのような微生物由来のもの
を用いてもよく、その生産のための手段は問わないが、
たとえばバチルスｓｐ、ＰＩ−７３（微工研菌寄第９８
４３号）から生産されたものを使用した場合には、特に
キチナーゼやキトサナーゼの活性が高いために、黴の細
胞壁の分解能に優れ、従って、ソフトコンタクトレンズ
からの黴の除去効果が非常に優れたものとなるのである
。As chitinase or chitosanase, for example, those derived from the genus Streptomyces or other microorganisms may be used, and the means for their production are not limited.
For example, Bacillus sp, PI-73
When using products produced from soft contact lenses (No. 43), the activity of chitinase and chitosanase was especially high, so the ability to decompose mold cell walls was excellent, and therefore the mold removal effect from soft contact lenses was excellent. It becomes something.
尚、上記バチルスｓｐ、ＰＩ−７５（微工研菌寄第９８
４３号）の菌学的性質は次のとおりである。In addition, the above-mentioned Bacillus sp, PI-75 (Feikoken Bacteria Collection No. 98)
The mycological properties of No. 43) are as follows.
（Ａ）形態学的性質
（ａ）菌の形態
桿菌
（ｂ）芽胞
楕円形、膨出
（ｃ）運動性
あり
（ｄ）ダラム染色性
不定
（Ｂ）次の各培地における生育状態
（ａ）肉汁寒天培地
３７°Ｃで２４〜９６時間培養を行ったところ、全周縁
が交円状のコロニーが形成され、時間の経過とともに盛
り上がってきた。(A) Morphological properties (a) Bacterial morphology Rod (b) Spores oval, swollen (c) Motile (d) Durham staining indeterminate (B) Growth status in each of the following media (a) Meat juice When cultured on an agar medium at 37° C. for 24 to 96 hours, colonies with intersecting circular edges were formed and swelled over time.
色は、２４時間培養時においてほぼ白濁色であるが、４
８時間培養時以降から薄黄色又は黄色を帯びてきた。The color is almost cloudy when cultured for 24 hours, but 4
After 8 hours of culture, the color became light yellow or yellowish.
生育状態は陽性と認められた。The growth status was recognized as positive.
（ｂ）肉汁液体撹拌培地
好気性であり、３７°Ｃで２４時間培養を行ったところ
、白濁色となった。(b) Meat juice liquid stirring medium was aerobic and became cloudy when cultured at 37°C for 24 hours.
生育状態は陽性と認められた。The growth status was recognized as positive.
（Ｃ）嫌気下での発育
発育せず
（Ｄ）生理学的性質
（ａ）カタラーゼの生成 ＋（ｂ） ＶＰ反
応 ＋Ｗ（ｃ） ＶＰブロスでの
ｐＨ４，８
（ｄ）グルコースからのガスの産生 −（ｅ）酸の産性
■ グルコース ＋
■ アラビノース −
■ キシロース −
■ マンニット ＋
（ｆ）ゼラチンの液化 ＋（ｇ）デンプン
の分解 ＋（ｈ）チロシンの分解
−（ｉ）クエン酸の利用性 −（Ｄ卵
黄反応 −
（ｋ）硝酸塩の還元 −（１）インドー
ル産生 −（ｍ） ｐＨ５，７での生育
十（ｎ）５％ＮａＣ１存在下での生育
−（ｏ）７％ＮａＣｌ存在下での生育 −（ｐ）
５０’Ｃでの生育 −尚、上記におい
て土は陽性、−は陰性、＋Ｗは弱陽性をそれぞれ意味す
る。(C) No growth under anaerobic conditions (D) Physiological properties (a) Production of catalase + (b) VP reaction +W (c) pH 4,8 in VP broth (d) Production of gas from glucose - (e) Acid productivity ■ Glucose + ■ Arabinose − ■ Xylose − ■ Mannitol + (f) Liquefaction of gelatin + (g) Decomposition of starch + (h) Decomposition of tyrosine
- (i) Utilization of citric acid - (D egg yolk reaction - (k) Reduction of nitrate - (1) Indole production - (m) Growth at pH 5, 7
Growth in the presence of 10(n) 5% NaCl
-(o) Growth in the presence of 7% NaCl -(p)
Growth at 50'C - In the above, soil means positive, - means negative, and +W means weakly positive.
又、上記実施例では、酵素の溶剤としてｐＨ６，８。In the above example, the pH of the enzyme solvent was 6.8.
０．０１モルのリン酸カリウム水溶液を用いたが、溶剤
の種類はこれに限定されない。Although a 0.01 mol potassium phosphate aqueous solution was used, the type of solvent is not limited thereto.
さらに、洗浄剤は上記実施例のような液状のものに限ら
ない。Furthermore, the cleaning agent is not limited to a liquid type as in the above embodiment.
その他、上記各実施例における操作手順、操作時におけ
る温度やソフトコンタクトレンズの浸漬時間等の条件も
限定されない。In addition, conditions such as the operating procedure, temperature during operation, and immersion time of the soft contact lens in each of the above-mentioned Examples are not limited.
（発明の効果）
叙上のように、本発明は、ソフトコンタクトレンズの洗
浄剤中にキチナーゼ、キトサナーゼ、β−１，３−グル
カナーゼのうちの少なくとも１つの酵素を含有したため
、仮にソフトコンタクトレンズに黴が発生しても、この
ような洗浄剤でソフトコンタクトレンズを洗浄すれば、
黴の細胞壁に含有されているキチン、キトサン、β−１
，３−グルカンのうちいずれかが分解されて黴が除去さ
れることとなる。(Effects of the Invention) As described above, the present invention contains at least one enzyme among chitinase, chitosanase, and β-1,3-glucanase in the cleaning agent for soft contact lenses. Even if mold develops, if you clean your soft contact lenses with a cleaning agent like this,
Chitin, chitosan, and β-1 contained in mold cell walls
, 3-glucans are decomposed and the mold is removed.
すなわち、本発明は、従来のように黴の発生し易い条件
を緩和するのではなく、あくまで黴自体を直接的に分解
してソフトコンタクトレンズから除去するものであるた
め、たとえ黴が発生したとしても、除去効果は十分に維
持され、その黴等の除去効果が従来に比べて格段優れた
ものになるという顕著な効果を有するに到った。In other words, the present invention does not alleviate the conditions under which mold easily occurs as in the past, but instead directly decomposes the mold itself and removes it from soft contact lenses. However, the removal effect was sufficiently maintained, and the removal effect of mold etc. was significantly superior to that of the conventional method.
Claims (1)
ーゼのうち、少なくとも１つの酵素を含有してなること
を特徴とするコンタクトレンズ用の洗浄剤。A cleaning agent for contact lenses, comprising at least one enzyme selected from chitinase, chitosanase, and β-1,3-glucanase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9377488A JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9377488A JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01265224A true JPH01265224A (en) | 1989-10-23 |
| JPH0576014B2 JPH0576014B2 (en) | 1993-10-21 |
Family
ID=14091769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9377488A Granted JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01265224A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6716970B2 (en) | 2000-04-21 | 2004-04-06 | Adjuvant Pharmaceuticals, Llc | Water soluble, randomly substituted partial N-partial O-acetylated chitosan, preserving compositions containing chitosan, and processes for making thereof |
| US8623400B2 (en) | 2011-07-08 | 2014-01-07 | National Chiao Tung University | Drug-carrying contact lens and method for fabricating the same |
| EP2746378A1 (en) * | 2012-12-18 | 2014-06-25 | EADS Deutschland GmbH | Cleaning mixture for removing or preventing deposits from insects on surfaces |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62285998A (en) * | 1986-06-02 | 1987-12-11 | マツダ株式会社 | Fungicidal agent on film |
-
1988
- 1988-04-15 JP JP9377488A patent/JPH01265224A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62285998A (en) * | 1986-06-02 | 1987-12-11 | マツダ株式会社 | Fungicidal agent on film |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6716970B2 (en) | 2000-04-21 | 2004-04-06 | Adjuvant Pharmaceuticals, Llc | Water soluble, randomly substituted partial N-partial O-acetylated chitosan, preserving compositions containing chitosan, and processes for making thereof |
| US7683039B2 (en) | 2000-04-21 | 2010-03-23 | Adjuvant Pharmaceuticals, Llc | Water soluble, randomly substituted partial N-, partial O-acetylated chitosan, preserving compositions containing chitosan, and processes for making thereof |
| US8623400B2 (en) | 2011-07-08 | 2014-01-07 | National Chiao Tung University | Drug-carrying contact lens and method for fabricating the same |
| EP2746378A1 (en) * | 2012-12-18 | 2014-06-25 | EADS Deutschland GmbH | Cleaning mixture for removing or preventing deposits from insects on surfaces |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0576014B2 (en) | 1993-10-21 |
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