JPH0576014B2 - - Google Patents
Info
- Publication number
- JPH0576014B2 JPH0576014B2 JP63093774A JP9377488A JPH0576014B2 JP H0576014 B2 JPH0576014 B2 JP H0576014B2 JP 63093774 A JP63093774 A JP 63093774A JP 9377488 A JP9377488 A JP 9377488A JP H0576014 B2 JPH0576014 B2 JP H0576014B2
- Authority
- JP
- Japan
- Prior art keywords
- mold
- contact lenses
- cleaning agent
- soft contact
- chitinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012459 cleaning agent Substances 0.000 claims description 24
- 108010022172 Chitinases Proteins 0.000 claims description 14
- 102000012286 Chitinases Human genes 0.000 claims description 14
- 108010089807 chitosanase Proteins 0.000 claims description 13
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 15
- 210000002421 cell wall Anatomy 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- 229920001661 Chitosan Polymers 0.000 description 8
- 108091005804 Peptidases Proteins 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 229920002101 Chitin Polymers 0.000 description 6
- 239000004365 Protease Substances 0.000 description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 5
- 241000228245 Aspergillus niger Species 0.000 description 4
- 101000763602 Manilkara zapota Thaumatin-like protein 1 Proteins 0.000 description 4
- 101000763586 Manilkara zapota Thaumatin-like protein 1a Proteins 0.000 description 4
- 101000966653 Musa acuminata Glucan endo-1,3-beta-glucosidase Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229920003002 synthetic resin Polymers 0.000 description 2
- 239000000057 synthetic resin Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 208000035859 Drug effect increased Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 102000040739 Secretory proteins Human genes 0.000 description 1
- 108091058545 Secretory proteins Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N Xylose Natural products O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 229940091771 aspergillus fumigatus Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/0005—Other compounding ingredients characterised by their effect
- C11D3/0078—Compositions for cleaning contact lenses, spectacles or lenses
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Eyeglasses (AREA)
- Detergent Compositions (AREA)
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明はコンタクトレンズ用の洗浄剤、さらに
詳しくは、いわゆるソフトコンタクトレンズと称
される合成樹脂製のコンタクトレンズに付着する
黴や細菌、或いはこれらの細胞壁を洗浄して除去
するための洗浄剤に関する。Detailed Description of the Invention (Industrial Application Field) The present invention relates to a cleaning agent for contact lenses, and more specifically, to cleaning agents for cleaning molds and bacteria that adhere to synthetic resin contact lenses called so-called soft contact lenses. This invention relates to a cleaning agent for cleaning and removing these cell walls.
（従来の技術）
一般に、コンタクトレンズにはガラス製のハー
ドコンタクトレンズと、ハイドロキシエチルメタ
クリレート等の合成樹脂からなる合成樹脂製のソ
フトコンタクトレンズとがあるが、後者のコンタ
クトレンズには黴や細菌が付着し易いという固有
の問題点があつた。(Prior art) In general, contact lenses include hard contact lenses made of glass and soft contact lenses made of synthetic resin such as hydroxyethyl methacrylate, but the latter contact lenses are susceptible to mold and bacteria. It had the inherent problem of easy adhesion.
すなわち、ソフトコンタクトレンズの場合、保
水性でキズが付き易く、涙中の分泌物（たとえば
蛋白質等）がレンズの表面に付着し、黴がソフト
コンタクトレンズの隙間に入つて増殖し易い。そ
して、一度増殖した黴はレンズに根づき、除去す
るのは困難であり、これが原因で失明する場合も
ごくまれにある。 That is, in the case of soft contact lenses, they tend to be easily scratched due to their water-retaining properties, secretions from tears (eg, proteins, etc.) adhere to the surface of the lenses, and mold easily enters the gaps in the soft contact lenses and grows. Once the mold grows, it takes root in the lens and is difficult to remove, and in very rare cases, this can cause blindness.
そこで、このような問題点を解決するために、
従来では、蛋白質分解酵素であるプロテアーゼを
含有したソフトコンタクトレンズ専用の洗浄剤も
開発されている。すなわち、このプロテアーゼが
涙分泌性蛋白質等を分解してソフトコンタクトレ
ンズから除去するのである。 Therefore, in order to solve such problems,
Conventionally, cleaning agents specifically for soft contact lenses have been developed that contain protease, which is a protein-degrading enzyme. That is, this protease decomposes lacrimal secretory proteins and the like and removes them from the soft contact lens.
（発明が解決しようとする問題点）
しかしながら、上記のような従来の洗浄剤は、
あくまでも分泌物（蛋白質等）を除去して黴等の
生育し易い条件を緩和するだけの機能しか有しな
いものであるため、黴の発生を完全に防止するこ
とはできない。特に、一旦黴が発生すると、その
発生した黴自体を除去する能力はかなり低いもの
であつた。(Problems to be solved by the invention) However, the conventional cleaning agents as mentioned above,
Since it only has the function of removing secretions (proteins, etc.) and easing conditions that favor the growth of mold, etc., it cannot completely prevent the growth of mold. In particular, once mold is generated, the ability to remove the mold itself is quite low.
本発明は、このような問題点を解決するために
なされたもので、黴の発生を略完全に防止し、仮
に黴が発生しても、その発生した黴を容易且つ確
実に除去することのできるコンタクトレンズ用の
洗浄剤を提供することを目的とする。 The present invention was made to solve these problems, and it is possible to almost completely prevent the generation of mold, and even if mold does occur, it can be easily and reliably removed. The purpose of the present invention is to provide a cleaning agent for contact lenses that can be used as a cleaning agent.
（問題点を解決するための手段）
本発明は、このような目的を達成するために、
上記ソフトコンタクトレンズに発生する黴を酵素
で分解することによつて黴を略完全に除去して上
記問題点を解決せんとするものである。(Means for solving the problems) In order to achieve such an object, the present invention has the following features:
The objective is to solve the above-mentioned problems by using enzymes to decompose the mold that forms on the soft contact lenses, thereby almost completely removing the mold.
すなわち、本発明の上記問題点を解決するため
の手段は、バチルスsp.PI−7S（微工研菌寄第9843
号）由来のキチナーゼ、又はキトサナーゼのう
ち、少なくともいずれか一方の酵素を洗浄剤中に
含有したことにある。尚、上記バチルスsp.PI−
7S（微工研菌寄第9843号）の菌学的性質は次のと
おりである。 That is, the means for solving the above-mentioned problems of the present invention are Bacillus sp.
The cleaning agent contains at least one of chitinase and chitosanase derived from No. 1). In addition, the above Bacillus sp.PI-
The mycological properties of 7S (Feikoken Bibori No. 9843) are as follows.
(A) 形態学的性質
(a) 菌の形態
桿菌
(b) 芽胞
楕円形、膨出
(c) 運動性
あり
(d) グラム染色性
不定
(B) 次の各培地における生育状態
(a) 肉汁寒天培地
37℃で24〜96時間培養を行つたところ、全周縁
が突円状のコロニーが形成され、時間の経過とと
もに盛り上がつてきた。(A) Morphological properties (a) Bacterial morphology Rod (b) Spores oval, bulging (c) Motile (d) Gram staining indeterminate (B) Growth status in each of the following media (a) Meat juice When cultured on an agar medium at 37°C for 24 to 96 hours, colonies with convex circular edges were formed and grew larger over time.
色は、24時間培養時においてほぼ白濁色である
が、48時間培養時以降から薄黄色又は黄色を帯び
てきた。 The color is almost cloudy when cultured for 24 hours, but becomes pale yellow or yellowish after 48 hours of culture.
生育状態は陽性と認められた。 The growth status was recognized as positive.
(b) 肉汁液体撹拌培地
好気性であり、37℃で24時間培養を行つたとこ
ろ、白濁色となつた。 (b) Meat juice liquid stirring medium It is aerobic and became cloudy when cultured at 37°C for 24 hours.
生育状態は陽性と認められた。 The growth status was recognized as positive.
(C) 嫌気下での発育
発育せず
(D) 生理学的性質
(a) カタラーゼの生成 ＋
(b) VP反応 ＋Ｗ
(c) VPブロスでのPH 4.8
(d) グルコースからのガスの産生 −
(e) 酸の産性
グルコース ＋
アラビノース −
キシロース −
マンニツト ＋
(f)ゼラチンの液化 ＋
(g)デンプンの分解 ＋
(h)チロシンの分解 −
(i)クエン酸の利用性 −
(j)卵黄反応 −
(k)硝酸塩の還元 −
(l)インドール産生 ＋
(m)PH5.7での生育 ＋
(n)５％NaCl存在下での生育 −
(o)７％NaCl存在下での生育 −
(p)50℃での生育 −
尚、上記において＋は陽性、−は陰性、＋Ｗは弱
陽性をそれぞれ意味する。(C) Growth under anaerobic conditions No growth (D) Physiological properties (a) Production of catalase + (b) VP reaction +W (c) PH in VP broth 4.8 (d) Production of gas from glucose − ( e) Acid production Glucose + Arabinose - Xylose - Mannite + (f) Liquefaction of gelatin + (g) Degradation of starch + (h) Degradation of tyrosine - (i) Availability of citric acid - (j) Egg yolk reaction - (k) Nitrate reduction − (l) Indole production + (m) Growth at PH5.7 + (n) Growth in the presence of 5% NaCl − (o) Growth in the presence of 7% NaCl − (p) Growth at 50°C - In the above, + means positive, - means negative, and +W means weakly positive.
（作用）
そして、上記ソフトコンタクトレンズに発生し
易い黴の細胞壁には、一般にキチンやキトサンが
含有されているが、上記洗浄剤には上記のような
酵素が含有されてなるため、その洗浄剤でソフト
コンタクトレンズを洗浄すると、洗浄剤に含有さ
れているバチルスsp.PI−7S（微工研菌寄第9843
号）由来のキチナーゼ、キトサナーゼのうち、少
なくとも１つの酵素が、上記黴の細胞壁に含有さ
れているキチンやキトサンのいずれかを分解し、
それによつて細胞壁が分解されてソフトコンタク
トレンズに付着した黴が除去されることとなるの
である。また、洗浄剤に含有されているキチナー
ゼ、キトサナーゼが、バチルスsp.PI−7S（微工研
菌寄第9843号）から生産されたものであり、その
キチナーゼ、キトサナーゼの活性が高いために、
黴の細胞壁の分解能に優れ、従つて、ソフトコン
タクトレンズからの黴の除去効率が非常に優れた
ものとなるのである。(Function) The cell walls of the mold that tends to grow on the above-mentioned soft contact lenses generally contain chitin and chitosan, but since the above-mentioned cleaning agent contains the above-mentioned enzymes, the cleaning agent When cleaning soft contact lenses with Bacillus sp.
At least one enzyme among chitinase and chitosanase derived from No. 1) decomposes either chitin or chitosan contained in the cell wall of the mold,
This breaks down the cell walls and removes the mold that has adhered to the soft contact lens. In addition, the chitinase and chitosanase contained in the cleaning agent are produced from Bacillus sp.
It has excellent resolution of mold cell walls, and therefore has a very high mold removal efficiency from soft contact lenses.
（実施例） 以下、本発明の実施例について説明する。(Example) Examples of the present invention will be described below.
実施例 １
本実施例におけるコンタクトレンズ用の洗浄剤
は、PH6.8、0.01モルのリン酸カリウム水溶液中
に、キチナーゼ、キトサナーゼ、β−１，３−グ
ルカナーゼ、及び蛋白質分解酵素が含有されて調
製された液状のものである。Example 1 A contact lens cleaning agent in this example was prepared by containing chitinase, chitosanase, β-1,3-glucanase, and protease in a 0.01 mol potassium phosphate aqueous solution at pH 6.8. It is in liquid form.
そして、このような組成からなるコンタクトレ
ンズ用の洗浄剤を使用する場合について説明する
と、先ずその洗浄剤の使用前に予めソフトコンタ
クトレンズに人為的に黴を付着させる。 When using a cleaning agent for contact lenses having such a composition, first, mold is artificially attached to soft contact lenses before using the cleaning agent.
すなわち、使い古したソフトコンタクトレンズ
をポリペプトンと酵母エキスとを含有する生理食
塩水に浸漬して十分に蛋白質と水分を吸着させ、
その裏面に黴の一種であるAspergillus niger
（IFO 4066）を塗布し、密閉容器に入れて37℃で
培養し、黴を十分に生育させる。 That is, a worn-out soft contact lens is immersed in physiological saline containing polypeptone and yeast extract to sufficiently absorb protein and water.
On the back side is Aspergillus niger, a type of mold.
(IFO 4066) and culture in a sealed container at 37°C to allow the mold to grow sufficiently.
次に、その黴の生育したソフトコンタクトレン
ズを100℃で５分間煮沸し、上記組成からなる洗
浄剤中に室温で約５〜６時間浸漬する。 Next, the mold-grown soft contact lens is boiled at 100° C. for 5 minutes, and then immersed in a cleaning agent having the above composition at room temperature for about 5 to 6 hours.
その後、浸漬後のソフトコンタクトレンズを蒸
留水で洗浄し、顕微鏡で観察したところ上記黴が
略完全に除去されていることが確認できた。 Thereafter, the soaked soft contact lenses were washed with distilled water, and when observed under a microscope, it was confirmed that the mold had been almost completely removed.
実施例 ２
本実施例は、洗浄剤中の酵素として、キチナー
ゼ、β−１，３−グルカナーゼ、及び蛋白質分解
酵素を含有させた実施例である。その他の条件、
すなわち溶剤や操作手順等については上記実施例
１と同様である。Example 2 This example is an example in which chitinase, β-1,3-glucanase, and proteolytic enzyme were contained as enzymes in the cleaning agent. other conditions,
That is, the solvent, operating procedure, etc. are the same as in Example 1 above.
実施例 ３
本実施例は、洗浄剤中の酵素として、キチナー
ゼ、キトサナーゼ、及びβ−１，３−グルカナー
ゼを含有させた実施例である。その他の条件は上
記実施例１と同様である。Example 3 This example is an example in which chitinase, chitosanase, and β-1,3-glucanase were contained as enzymes in the cleaning agent. Other conditions were the same as in Example 1 above.
実施例 ４
本実施例は、洗浄剤中の酵素として、キチナー
ゼ及びβ−１，３−グルカナーゼを含有させた実
施例である。その他の条件は上記実施例１と同様
である。Example 4 This example is an example in which chitinase and β-1,3-glucanase were contained as enzymes in the cleaning agent. Other conditions were the same as in Example 1 above.
実施例 ５
本実施例は、洗浄剤中の酵素として、キチナー
ゼのみを含有させた実施例である。その他の条件
は上記実施例１と同様である。Example 5 This example is an example in which only chitinase was contained as the enzyme in the cleaning agent. Other conditions were the same as in Example 1 above.
実施例 ６
本実施例は、洗浄剤中の酵素として、キトサナ
ーゼのみを含有させた実施例である。その他の条
件は上記実施例１と同様である。Example 6 This example is an example in which only chitosanase was contained as the enzyme in the detergent. Other conditions were the same as in Example 1 above.
以上のような実施例１〜６について黴の除去効
果について確認したところ、次のような順位とな
つた。 When the mold removal effects of Examples 1 to 6 as described above were confirmed, the following rankings were obtained.
１≧２＞３≧４＞５＞６比較例
尚、それぞれの数字は各実施例の数字を意味す
る。又、比較例とは、酵素を含まない溶剤のみの
液で上記と同様の操作で黴を除去した場合につい
ての結果である。 1≧2>3≧4>5>6 Comparative Example In addition, each number means the number of each example. In addition, the comparative example is the result of removing mold using the same procedure as above using a solution containing only a solvent and no enzyme.
上記の結果からも明らかなように、溶剤中に含
有される酵素の種類の多いもの程、効果が強いこ
とが認められた。これは、細胞壁中にキチン、キ
トサン、β−１，３−グルカン等の複数の成分が
含まれているため、酵素も複数使用されればその
分効果が増大することが裏付けられるのである。 As is clear from the above results, it was found that the more types of enzymes contained in the solvent, the stronger the effect. This is because the cell wall contains multiple components such as chitin, chitosan, and β-1,3-glucan, which proves that if multiple enzymes are used, the effect will increase accordingly.
又、蛋白質分解酵素を含有させれば、その分効
果が増大した。これは、細胞壁中に若干ながらも
蛋白質が含有されているための推測される。 Furthermore, when a proteolytic enzyme was included, the effect increased accordingly. This is presumably because the cell wall contains a small amount of protein.
さらに、上記結果においては、キトサナーゼの
効果は弱いものであつた。これは、上記実施例に
用いられた黴であるAspergillus nigerの細胞壁
中のキトサンの含有量が少ないためであり、たと
えばキトサンを大量に含む他の黴を使用すれば効
果は増大すると認められる。 Furthermore, in the above results, the effect of chitosanase was weak. This is because the chitosan content in the cell wall of Aspergillus niger, the mold used in the above examples, is low, and it is recognized that the effect will be increased if, for example, another mold containing a large amount of chitosan is used.
尚、上記各実施例においては、黴として
Aspergillus nigerを使用したが、使用する黴の
種類はこれに限られるものではない。すなわち、
上記ソフトコンタクトレンズに発生し易い黴とし
ては、上記Aspergillus nigerの他、一般に
Aspergillus fumigatus，Aspergillus flavus，
Candida parapailosis，Candida albicans等の
ものが知られているが、このような黴や菌はいず
れもその細胞壁にキチンやキトサン等を含有して
いるため、上記各実施例のような酵素を含有する
洗浄剤によつて黴や細菌の除去効果が認められる
のである。ただし、対象となる黴等はこれらに限
定されるものではない。 In addition, in each of the above examples, as mold
Although Aspergillus niger was used, the type of mold used is not limited to this. That is,
In addition to the above-mentioned Aspergillus niger, the mold that tends to grow on soft contact lenses is
Aspergillus fumigatus, Aspergillus flavus,
Candida parapailosis, Candida albicans, etc. are known, but since all of these molds and bacteria contain chitin, chitosan, etc. in their cell walls, cleaning agents containing enzymes such as those in the above examples may be used. The effect of removing mold and bacteria is recognized depending on the agent. However, the target molds, etc. are not limited to these.
又、上記実施例１や実施例２からも明らかなよ
うに、蛋白質分解酵素の含有によつて効果の増大
が認められたが、蛋白質分解酵素を含有させるこ
とは本発明においては条件ではない。 Furthermore, as is clear from Examples 1 and 2, the effect was increased by the inclusion of a protease, but the inclusion of a protease is not a condition for the present invention.
さらに、上記各実施例からも明らかなように、
酵素としては、キチナーゼ、キトサナーゼ、β−
１，３−グルカナーゼがすべて含有されている必
要は必ずしもなく、黴の細胞壁に含有されている
キチン、キトサンの少なくともいずれかが分解さ
れれば黴自体も分解されること、及びその分解能
が優れているとの観点より、要は洗浄剤にはバチ
ルスsp.PI−7S（微工研菌寄第9843号）由来のキチ
ナーゼ、キトサナーゼのうち少なくともいずれか
一方の酵素が含有されていればよいのである。 Furthermore, as is clear from the above examples,
Enzymes include chitinase, chitosanase, β-
It is not necessary that all 1,3-glucanases be contained; if at least one of chitin and chitosan contained in the mold cell wall is decomposed, the mold itself will be decomposed, and the decomposition ability is excellent. From the viewpoint that the cleaning agent contains at least one of chitinase and chitosanase derived from Bacillus sp. PI-7S (Feikoken Bacteria No. 9843) .
又、上記実施例では、酵素の溶剤としてPH6.8、
0.01モルのリン酸カリウム水溶液を用いたが、溶
剤の種類はこれに限定されない。 In addition, in the above example, pH6.8,
Although a 0.01 mol potassium phosphate aqueous solution was used, the type of solvent is not limited thereto.
さらに、洗浄剤は上記実施例のような液状のも
のに限らない。 Furthermore, the cleaning agent is not limited to a liquid type as in the above embodiment.
その他、上記各実施例における操作手順、操作
時における温度やソフトコンタクトレンズの浸漬
時間等の条件も限定されない。 In addition, conditions such as the operating procedure, temperature during operation, and immersion time of the soft contact lens in each of the above-mentioned Examples are not limited.
（発明の効果）
叙上のように、本発明は、ソフトコンタクトレ
ンズの洗浄中にバチルスsp.PI−7S（微工研菌寄第
9843号）由来のキチナーゼ、キトサナーゼのうち
の少なくとも１つの酵素を含有したため、仮にソ
フトコンタクトレンズに黴が発生しても、このよ
うな洗浄剤でソフトコンタクトレンズを洗浄すれ
ば、黴の細胞壁に含有されているキチン、キトサ
ン、のうちいずれかが分解されて黴が除去される
こととなる。(Effects of the Invention) As described above, the present invention has the advantage that Bacillus sp.
Contains at least one enzyme of chitinase and chitosanase derived from 9843), so even if mold develops on soft contact lenses, cleaning the soft contact lenses with such a cleaning agent will eliminate the mold contained in the cell walls. Either chitin or chitosan is decomposed and the mold is removed.
すなわち、本発明は、従来のように黴の発生し
易い条件を緩和するのではなく、あくまで黴自体
を直接的に分解してソフトコンタクトレンズから
除去するものであるため、たとえ黴が発生したと
しても、除去効果は十分に維持され、その黴等の
除去効果が従来に比べて格段優れたものになると
いう顕著な効果を有するに到つた。また、含有さ
れているキチナーゼ、キトサナーゼがバチルス
sp.PI−7S（微工研菌寄第9843号）から生産された
ものであるため、特にキチナーゼやキトサナーゼ
の活性が高く、黴の細胞壁の分解能に優れ、従つ
て、ソフトコンタクトレンズからの黴の除去効率
が非常に優れたものとなるという効果がある。 In other words, the present invention does not alleviate the conditions under which mold easily occurs as in the past, but instead directly decomposes the mold itself and removes it from soft contact lenses. However, the removal effect was sufficiently maintained, and the removal effect of mold etc. was significantly superior to that of the conventional method. In addition, the chitinase and chitosanase contained in Bacillus
Since it is produced from sp. PI-7S (Feikoken Bacteria No. 9843), it has particularly high chitinase and chitosanase activities, and has excellent ability to decompose mold cell walls, thus preventing mold from soft contact lenses. This has the effect of making the removal efficiency extremely high.
Claims (1)
来のキチナーゼ、又はキトサナーゼのうち、少な
くともいずれか一方の酵素を含有してなることを
特徴とするコンタクトレンズ用の洗浄剤。1. A cleaning agent for contact lenses, characterized in that it contains at least one of chitinase and chitosanase derived from Bacillus sp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9377488A JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9377488A JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01265224A JPH01265224A (en) | 1989-10-23 |
| JPH0576014B2 true JPH0576014B2 (en) | 1993-10-21 |
Family
ID=14091769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9377488A Granted JPH01265224A (en) | 1988-04-15 | 1988-04-15 | Cleaner for contact lens |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01265224A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6716970B2 (en) | 2000-04-21 | 2004-04-06 | Adjuvant Pharmaceuticals, Llc | Water soluble, randomly substituted partial N-partial O-acetylated chitosan, preserving compositions containing chitosan, and processes for making thereof |
| TW201302244A (en) | 2011-07-08 | 2013-01-16 | Univ Nat Chiao Tung | Drug-carrying contact lens and manufacturing method thereof |
| DE102012112519A1 (en) * | 2012-12-18 | 2014-06-18 | Eads Deutschland Gmbh | Cleaning mixture for removing or avoiding insect deposits on surfaces |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62285998A (en) * | 1986-06-02 | 1987-12-11 | マツダ株式会社 | Fungicidal agent on film |
-
1988
- 1988-04-15 JP JP9377488A patent/JPH01265224A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62285998A (en) * | 1986-06-02 | 1987-12-11 | マツダ株式会社 | Fungicidal agent on film |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01265224A (en) | 1989-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4710313A (en) | Detergent composition for contact lenses | |
| AU651009B2 (en) | Method for cleaning a contact lens | |
| JPH08509367A (en) | L-amino acid oxidase | |
| JPH0576014B2 (en) | ||
| Vršanská et al. | Lysis of intact yeast cells and isolated cell walls by an inducible enzyme system of Arthrobacter GJM‐1 | |
| US5785767A (en) | Cleaning and disinfecting solution for contact lens and method of cleaning and disinfecting contact lens using the solution | |
| JP3655359B2 (en) | Composition for cleaning and disinfecting hydrous soft contact lenses | |
| US3330738A (en) | Production of enzyme complex by cytophaga (flavobacterium) ncib 9497 | |
| JPH05142502A (en) | Cleaning composition for contact lens containing antibacterial solid grain | |
| JP2935226B2 (en) | Novel chitosanase, method for producing the chitosanase, and method for producing chitosan oligosaccharide using the chitosanase | |
| JP3664804B2 (en) | Low-temperature alkaline protease X, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
| US5354681A (en) | Enzyme complex hydrolyzing bacterial cell walls derived from nocardiopsis dassonvillei | |
| JP2731212B2 (en) | Novel cellulase and method for producing the same | |
| JP3664802B2 (en) | Low-temperature alkaline protease, microorganism producing the same, method for producing the same, detergent composition containing the enzyme, and enzyme preparation for food processing | |
| JP3592794B2 (en) | Low-temperature alkaline protease, microorganism producing the same, and method for producing the alkaline protease | |
| JP3030755B2 (en) | Method for producing enzyme-immobilizing carrier | |
| JP3664803B2 (en) | Low temperature alkaline protease T16, microorganism producing the same, method for producing the same, detergent composition containing the enzyme and enzyme preparation for food processing | |
| JP2985018B2 (en) | New microorganism | |
| JPH0379987B2 (en) | ||
| JPH03191320A (en) | Detergent for contact lens | |
| KR860000797A (en) | How to clean and remove attached algae | |
| JP3595024B2 (en) | Low-temperature optimal protease, microorganism producing the same, and method for producing the protease | |
| JPS61280278A (en) | Alkali protease | |
| JP3000310B2 (en) | Carboxymethylcellulase and microorganism producing the same | |
| JP2000023667A (en) | Alkali-resistant amylase |