JP7883986B2 - 最適化されたタンパク質リンカーおよび使用方法 - Google Patents

最適化されたタンパク質リンカーおよび使用方法

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Publication number
JP7883986B2
JP7883986B2 JP2023504348A JP2023504348A JP7883986B2 JP 7883986 B2 JP7883986 B2 JP 7883986B2 JP 2023504348 A JP2023504348 A JP 2023504348A JP 2023504348 A JP2023504348 A JP 2023504348A JP 7883986 B2 JP7883986 B2 JP 7883986B2
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Japan
Prior art keywords
nucleic acid
domain
activity
fusion protein
deaminase domain
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JP2023504348A
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English (en)
Japanese (ja)
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JP2023536408A5 (enExample
JP2023536408A (ja
Inventor
ガフィー，シャロン・リー
ワッツ，ジョセフ・マシュー
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Pairwise Plants Services Inc
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Pairwise Plants Services Inc
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Publication of JP2023536408A5 publication Critical patent/JP2023536408A5/ja
Priority to JP2026031673A priority Critical patent/JP2026108651A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04002—Adenine deaminase (3.5.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004—Adenosine deaminase (3.5.4.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2023504348A 2020-07-21 2021-07-21 最適化されたタンパク質リンカーおよび使用方法 Active JP7883986B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2026031673A JP2026108651A (ja) 2020-07-21 2026-02-27 最適化されたタンパク質リンカーおよび使用方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063054449P 2020-07-21 2020-07-21
US63/054,449 2020-07-21
PCT/US2021/042457 WO2022020407A1 (en) 2020-07-21 2021-07-21 Optimized protein linkers and methods of use

Related Child Applications (1)

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JP2026031673A Division JP2026108651A (ja) 2020-07-21 2026-02-27 最適化されたタンパク質リンカーおよび使用方法

Publications (3)

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JP2023536408A JP2023536408A (ja) 2023-08-25
JP2023536408A5 JP2023536408A5 (enExample) 2024-07-30
JP7883986B2 true JP7883986B2 (ja) 2026-07-02

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JP2023504348A Active JP7883986B2 (ja) 2020-07-21 2021-07-21 最適化されたタンパク質リンカーおよび使用方法
JP2026031673A Pending JP2026108651A (ja) 2020-07-21 2026-02-27 最適化されたタンパク質リンカーおよび使用方法

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Country Status (7)

Country Link
US (2) US11718838B2 (enExample)
EP (1) EP4185699B1 (enExample)
JP (2) JP7883986B2 (enExample)
CN (1) CN116391034A (enExample)
AU (1) AU2021313163B2 (enExample)
CA (1) CA3189443A1 (enExample)
WO (1) WO2022020407A1 (enExample)

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CN120591235A (zh) * 2019-07-19 2025-09-05 成对植物服务股份有限公司 经优化的蛋白质连接体以及使用方法
EP4499819A1 (en) 2022-03-30 2025-02-05 Basf Agricultural Solutions Seed Us Llc Optimized base editors
CN114634923B (zh) * 2022-04-07 2024-02-23 尧唐(上海)生物科技有限公司 腺苷脱氨酶、碱基编辑器融合蛋白、碱基编辑器系统及用途
WO2025005684A1 (ko) * 2023-06-28 2025-01-02 주식회사 그린진 닉카제 매개된 식물 dna의 염기 교정
WO2026143185A1 (en) * 2024-12-23 2026-07-02 Ensoma Aps Engineered rna-guided dna-binding polypeptides
CN122256307A (zh) * 2026-05-26 2026-06-23 三亚中国农业科学院国家南繁研究院 新型尿嘧啶DNA糖基化酶Rco及其在碱基编辑中的应用

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CN109957569A (zh) 2017-12-22 2019-07-02 中国科学院遗传与发育生物学研究所 基于cpf1蛋白的碱基编辑系统和方法
JP2020511970A (ja) 2017-03-23 2020-04-23 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子

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US6747137B1 (en) 1998-02-13 2004-06-08 Genome Therapeutics Corporation Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics
US7214786B2 (en) 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
FR2862659B1 (fr) 2003-11-21 2006-02-10 Pasteur Institut Genome de legionella pneumophila souche paris- applications diagnostiques et epidemiologiques
EP2175714A4 (en) 2007-07-10 2011-01-26 Monsanto Technology Llc TRANSGENIC PLANTS WITH IMPROVED AGRONOMIC CHARACTERISTICS
US20110182862A1 (en) 2009-04-03 2011-07-28 Green Wayne A Endophytic fungus and uses therefor
WO2014020129A2 (en) 2012-08-01 2014-02-06 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Allatostatin-b peptides for inducing the settlement of lophotrochozoan marine larvae
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2018027078A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harard College Adenosine nucleobase editors and uses thereof
KR102569848B1 (ko) * 2016-12-23 2023-08-25 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Pcsk9의 유전자 편집
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2019051097A1 (en) 2017-09-08 2019-03-14 The Regents Of The University Of California RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USING SAME
CN110157727A (zh) * 2017-12-21 2019-08-23 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
WO2019126774A1 (en) * 2017-12-22 2019-06-27 The Broad Institute, Inc. Novel crispr enzymes and systems
CN109295186B (zh) * 2018-09-30 2023-10-03 中山大学 一种基于全基因组测序检测腺嘌呤单碱基编辑系统脱靶效应的方法及其在基因编辑中的应用
CN110029096B (zh) * 2019-05-09 2023-05-12 上海科技大学 一种腺嘌呤碱基编辑工具及其用途
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JP2020511970A (ja) 2017-03-23 2020-04-23 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸によってプログラム可能なｄｎａ結合蛋白質を含む核酸塩基編集因子
CN109957569A (zh) 2017-12-22 2019-07-02 中国科学院遗传与发育生物学研究所 基于cpf1蛋白的碱基编辑系统和方法

Also Published As

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AU2021313163A1 (en) 2023-02-16
US20240002821A1 (en) 2024-01-04
JP2026108651A (ja) 2026-06-30
US12110517B2 (en) 2024-10-08
CN116391034A (zh) 2023-07-04
EP4185699B1 (en) 2026-07-22
US11718838B2 (en) 2023-08-08
US20220025346A1 (en) 2022-01-27
WO2022020407A1 (en) 2022-01-27
AU2021313163B2 (en) 2025-11-27
CA3189443A1 (en) 2022-01-27
JP2023536408A (ja) 2023-08-25
EP4185699A1 (en) 2023-05-31

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