CA3189443A1 - Optimized protein linkers and methods of use - Google Patents
Optimized protein linkers and methods of useInfo
- Publication number
- CA3189443A1 CA3189443A1 CA3189443A CA3189443A CA3189443A1 CA 3189443 A1 CA3189443 A1 CA 3189443A1 CA 3189443 A CA3189443 A CA 3189443A CA 3189443 A CA3189443 A CA 3189443A CA 3189443 A1 CA3189443 A1 CA 3189443A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- domain
- activity
- fusion protein
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04002—Adenine deaminase (3.5.4.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04004—Adenosine deaminase (3.5.4.4)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063054449P | 2020-07-21 | 2020-07-21 | |
| US63/054,449 | 2020-07-21 | ||
| PCT/US2021/042457 WO2022020407A1 (en) | 2020-07-21 | 2021-07-21 | Optimized protein linkers and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3189443A1 true CA3189443A1 (en) | 2022-01-27 |
Family
ID=77564139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3189443A Pending CA3189443A1 (en) | 2020-07-21 | 2021-07-21 | Optimized protein linkers and methods of use |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11718838B2 (enExample) |
| EP (1) | EP4185699B1 (enExample) |
| JP (2) | JP7883986B2 (enExample) |
| CN (1) | CN116391034A (enExample) |
| AU (1) | AU2021313163B2 (enExample) |
| CA (1) | CA3189443A1 (enExample) |
| WO (1) | WO2022020407A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120591235A (zh) * | 2019-07-19 | 2025-09-05 | 成对植物服务股份有限公司 | 经优化的蛋白质连接体以及使用方法 |
| EP4499819A1 (en) | 2022-03-30 | 2025-02-05 | Basf Agricultural Solutions Seed Us Llc | Optimized base editors |
| CN114634923B (zh) * | 2022-04-07 | 2024-02-23 | 尧唐(上海)生物科技有限公司 | 腺苷脱氨酶、碱基编辑器融合蛋白、碱基编辑器系统及用途 |
| WO2025005684A1 (ko) * | 2023-06-28 | 2025-01-02 | 주식회사 그린진 | 닉카제 매개된 식물 dna의 염기 교정 |
| WO2026143185A1 (en) * | 2024-12-23 | 2026-07-02 | Ensoma Aps | Engineered rna-guided dna-binding polypeptides |
| CN122256307A (zh) * | 2026-05-26 | 2026-06-23 | 三亚中国农业科学院国家南繁研究院 | 新型尿嘧啶DNA糖基化酶Rco及其在碱基编辑中的应用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6747137B1 (en) | 1998-02-13 | 2004-06-08 | Genome Therapeutics Corporation | Nucleic acid sequences relating to Candida albicans for diagnostics and therapeutics |
| US7214786B2 (en) | 2000-12-14 | 2007-05-08 | Kovalic David K | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| FR2862659B1 (fr) | 2003-11-21 | 2006-02-10 | Pasteur Institut | Genome de legionella pneumophila souche paris- applications diagnostiques et epidemiologiques |
| EP2175714A4 (en) | 2007-07-10 | 2011-01-26 | Monsanto Technology Llc | TRANSGENIC PLANTS WITH IMPROVED AGRONOMIC CHARACTERISTICS |
| US20110182862A1 (en) | 2009-04-03 | 2011-07-28 | Green Wayne A | Endophytic fungus and uses therefor |
| WO2014020129A2 (en) | 2012-08-01 | 2014-02-06 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Allatostatin-b peptides for inducing the settlement of lophotrochozoan marine larvae |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| KR102569848B1 (ko) * | 2016-12-23 | 2023-08-25 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | Pcsk9의 유전자 편집 |
| KR20190123328A (ko) | 2017-03-09 | 2019-10-31 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 암 백신 |
| IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
| WO2019051097A1 (en) | 2017-09-08 | 2019-03-14 | The Regents Of The University Of California | RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USING SAME |
| CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
| WO2019126774A1 (en) * | 2017-12-22 | 2019-06-27 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| AR114014A1 (es) | 2017-12-22 | 2020-07-08 | Inst Genetics & Developmental Biology Cas | Sistema de edición de bases y método basado en proteína cpf1 |
| CN109295186B (zh) * | 2018-09-30 | 2023-10-03 | 中山大学 | 一种基于全基因组测序检测腺嘌呤单碱基编辑系统脱靶效应的方法及其在基因编辑中的应用 |
| CN110029096B (zh) * | 2019-05-09 | 2023-05-12 | 上海科技大学 | 一种腺嘌呤碱基编辑工具及其用途 |
| CN120591235A (zh) | 2019-07-19 | 2025-09-05 | 成对植物服务股份有限公司 | 经优化的蛋白质连接体以及使用方法 |
-
2021
- 2021-07-21 CN CN202180061805.5A patent/CN116391034A/zh active Pending
- 2021-07-21 AU AU2021313163A patent/AU2021313163B2/en active Active
- 2021-07-21 US US17/381,465 patent/US11718838B2/en active Active
- 2021-07-21 WO PCT/US2021/042457 patent/WO2022020407A1/en not_active Ceased
- 2021-07-21 CA CA3189443A patent/CA3189443A1/en active Pending
- 2021-07-21 EP EP21763165.4A patent/EP4185699B1/en active Active
- 2021-07-21 JP JP2023504348A patent/JP7883986B2/ja active Active
-
2023
- 2023-06-14 US US18/334,407 patent/US12110517B2/en active Active
-
2026
- 2026-02-27 JP JP2026031673A patent/JP2026108651A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2021313163A1 (en) | 2023-02-16 |
| US20240002821A1 (en) | 2024-01-04 |
| JP2026108651A (ja) | 2026-06-30 |
| US12110517B2 (en) | 2024-10-08 |
| CN116391034A (zh) | 2023-07-04 |
| EP4185699B1 (en) | 2026-07-22 |
| US11718838B2 (en) | 2023-08-08 |
| US20220025346A1 (en) | 2022-01-27 |
| WO2022020407A1 (en) | 2022-01-27 |
| AU2021313163B2 (en) | 2025-11-27 |
| JP2023536408A (ja) | 2023-08-25 |
| JP7883986B2 (ja) | 2026-07-02 |
| EP4185699A1 (en) | 2023-05-31 |
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