JP7872234B2 - ライゲーションカップルドｐｃｒの方法 - Google Patents

ライゲーションカップルドｐｃｒの方法

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Publication number
JP7872234B2
JP7872234B2 JP2022569537A JP2022569537A JP7872234B2 JP 7872234 B2 JP7872234 B2 JP 7872234B2 JP 2022569537 A JP2022569537 A JP 2022569537A JP 2022569537 A JP2022569537 A JP 2022569537A JP 7872234 B2 JP7872234 B2 JP 7872234B2
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adapter
indexing
item
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JP2023525880A (ja
JP2023525880A5 (enExample
Inventor
ブラディミル マカロフ，
ジョーダン ローズフィグラ，
ビタ ラハン，
ロバート ディー． ステットフェルド，
Original Assignee
インテグレイテッド ディーエヌエー テクノロジーズ， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/149—Concentration of an enzyme
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022569537A 2020-05-15 2021-05-17 ライゲーションカップルドｐｃｒの方法 Active JP7872234B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063025738P 2020-05-15 2020-05-15
US63/025,738 2020-05-15
PCT/US2021/032824 WO2021232023A2 (en) 2020-05-15 2021-05-17 Methods for ligation-coupled-pcr

Publications (3)

Publication Number Publication Date
JP2023525880A JP2023525880A (ja) 2023-06-19
JP2023525880A5 JP2023525880A5 (enExample) 2024-05-24
JP7872234B2 true JP7872234B2 (ja) 2026-06-09

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JP2022569537A Active JP7872234B2 (ja) 2020-05-15 2021-05-17 ライゲーションカップルドｐｃｒの方法

Country Status (8)

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US (1) US20230257805A1 (enExample)
EP (1) EP4150106A4 (enExample)
JP (1) JP7872234B2 (enExample)
KR (1) KR20230012554A (enExample)
CN (1) CN116249775A (enExample)
AU (1) AU2021270596A1 (enExample)
CA (1) CA3178211A1 (enExample)
WO (1) WO2021232023A2 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11104941B2 (en) * 2018-09-28 2021-08-31 Bioo Scientific Corporation 5′ adapter comprising an internal 5′-5′ linkage
EP4342999B1 (en) * 2020-01-16 2025-06-11 DNAe Diagnostics Limited Compositions, kits and methods for isolating target polynucleotides
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法
EP4499860A1 (en) 2022-03-25 2025-02-05 Bioo Scientific Corporation Methods, compositions and kits for inhibiting formation of adapter dimers
JPWO2025079564A1 (enExample) * 2023-10-13 2025-04-17

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031954A1 (en) 2012-08-23 2014-02-27 Tufts University Homopolymer mediated nucleic acid amplification
JP2017504360A (ja) 2014-01-31 2017-02-09 スウィフト バイオサイエンシズ， インク．Ｓｗｉｆｔ Ｂｉｏｓｃｉｅｎｃｅｓ， Ｉｎｃ． Ｄｎａ基質を処理するための改善された方法
WO2018048957A2 (en) 2016-09-06 2018-03-15 Swift Biosciences, Inc. Normalization of ngs library concentration
WO2019157034A1 (en) 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
CN111041026A (zh) 2019-12-26 2020-04-21 北京优迅医学检验实验室有限公司 一种高通量测序用核酸接头和文库构建方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10208338B2 (en) 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
AU2018254190B2 (en) * 2017-04-17 2024-05-30 Guoliang Fu Methods, compositions, and kits for preparing nucleic acid libraries
KR20250047324A (ko) * 2017-08-07 2025-04-03 더 존스 홉킨스 유니버시티 암을 평가하고 치료하기 위한 방법 및 재료
US11447818B2 (en) * 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
EP3814497A4 (en) * 2018-05-18 2022-03-02 Twist Bioscience Corporation POLYNUCLEOTIDES, REAGENTS AND METHODS FOR NUCLEIC ACID HYBRIDIZATION
CA3253118A1 (en) * 2018-06-04 2026-03-02 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
JP7431802B2 (ja) * 2018-08-15 2024-02-15 イルミナ インコーポレイテッド ライブラリー富化を改善するための組成物および方法

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014031954A1 (en) 2012-08-23 2014-02-27 Tufts University Homopolymer mediated nucleic acid amplification
JP2017504360A (ja) 2014-01-31 2017-02-09 スウィフト バイオサイエンシズ， インク．Ｓｗｉｆｔ Ｂｉｏｓｃｉｅｎｃｅｓ， Ｉｎｃ． Ｄｎａ基質を処理するための改善された方法
US10316357B2 (en) 2014-01-31 2019-06-11 Swift Biosciences, Inc. Compositions and methods for enhanced adapter ligation
WO2018048957A2 (en) 2016-09-06 2018-03-15 Swift Biosciences, Inc. Normalization of ngs library concentration
WO2019157034A1 (en) 2018-02-07 2019-08-15 Nugen Technologies, Inc. Library preparation
CN111041026A (zh) 2019-12-26 2020-04-21 北京优迅医学检验实验室有限公司 一种高通量测序用核酸接头和文库构建方法

Also Published As

Publication number Publication date
AU2021270596A1 (en) 2022-12-15
US20230257805A1 (en) 2023-08-17
WO2021232023A3 (en) 2021-12-23
KR20230012554A (ko) 2023-01-26
JP2023525880A (ja) 2023-06-19
WO2021232023A2 (en) 2021-11-18
CA3178211A1 (en) 2021-11-18
CN116249775A (zh) 2023-06-09
EP4150106A2 (en) 2023-03-22
EP4150106A4 (en) 2024-07-10

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