JP7828901B2 - 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法 - Google Patents
試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法Info
- Publication number
- JP7828901B2 JP7828901B2 JP2022575771A JP2022575771A JP7828901B2 JP 7828901 B2 JP7828901 B2 JP 7828901B2 JP 2022575771 A JP2022575771 A JP 2022575771A JP 2022575771 A JP2022575771 A JP 2022575771A JP 7828901 B2 JP7828901 B2 JP 7828901B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2020/067010 WO2020254519A1 (en) | 2019-06-18 | 2020-06-18 | Method of signal encoding of analytes in a sample |
| EPPCT/EP2020/067010 | 2020-06-18 | ||
| US202063127910P | 2020-12-18 | 2020-12-18 | |
| US63/127,910 | 2020-12-18 | ||
| US202063129942P | 2020-12-23 | 2020-12-23 | |
| US63/129,942 | 2020-12-23 | ||
| PCT/EP2021/066668 WO2021255263A2 (en) | 2020-06-18 | 2021-06-18 | Multiplex method for detecting different analytes and different subgroups/variations of an analyte in a sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023530889A JP2023530889A (ja) | 2023-07-20 |
| JP2023530889A5 JP2023530889A5 (https=) | 2024-06-12 |
| JP7828901B2 true JP7828901B2 (ja) | 2026-03-12 |
Family
ID=79268536
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022575771A Active JP7828901B2 (ja) | 2020-06-18 | 2021-06-18 | 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法 |
| JP2022575762A Pending JP2023529682A (ja) | 2020-06-18 | 2021-06-18 | 試料中の異なる分析物を検出するための多重的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022575762A Pending JP2023529682A (ja) | 2020-06-18 | 2021-06-18 | 試料中の異なる分析物を検出するための多重的方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US12492427B2 (https=) |
| EP (2) | EP4168575A1 (https=) |
| JP (2) | JP7828901B2 (https=) |
| KR (2) | KR20230023646A (https=) |
| CN (2) | CN115702249A (https=) |
| AU (2) | AU2021294274A1 (https=) |
| CA (2) | CA3181737A1 (https=) |
| WO (2) | WO2021255244A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12492427B2 (en) * | 2020-06-18 | 2025-12-09 | Resolve Biosciences Gmbh | Multiplex method for detecting different analytes in a sample |
| DE102022131450A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131442A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Zuordnen von Bildbereichen einer Bildfolge zu Ergebnisklassen mittels eines Analytdaten-Auswertesystems mit einem Verarbeitungsmodells |
| DE102022131447A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131441A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131448A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131445A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131446A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131451A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge |
| DE102022131444A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren zum Identifizieren von Analyten in einer Bildfolge |
| DE102022131449A1 (de) | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| KR20250154509A (ko) * | 2023-03-01 | 2025-10-28 | 리졸브 바이오사이언시즈 게엠베하 | 다양한 응용에서 다중 분석물의 병렬 검출을 위한 복합체 형성 |
| WO2024256715A1 (en) * | 2023-06-16 | 2024-12-19 | Resolve Biosciences Gmbh | Reagent for in situ hybridization quality control |
| DE102023122213A1 (de) | 2023-08-18 | 2025-02-20 | Carl Zeiss Microscopy Gmbh | Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen |
| WO2025093690A1 (en) | 2023-11-02 | 2025-05-08 | Resolve Biosciences Gmbh | Method for preparing tissue sections |
| WO2025099313A1 (en) | 2023-11-10 | 2025-05-15 | Resolve Biosciences Gmbh | Reducing background fluorescence in single molecule fluorescence in situ hybridization applications |
| WO2025125442A1 (en) * | 2023-12-13 | 2025-06-19 | Resolve Biosciences Gmbh | High resolution multiplex method for detecting at least two targets |
| WO2025132546A1 (en) | 2023-12-19 | 2025-06-26 | Resolve Biosciences Gmbh | Method for reducing nonspecific signals in in-situ hybridization assays |
| WO2025250686A1 (en) * | 2024-05-28 | 2025-12-04 | The Regents Of The University Of California | Methods and systems for parallel spatially resolved single-cell profiling |
| WO2026037951A1 (en) | 2024-08-16 | 2026-02-19 | Resolve Biosciences Gmbh | Compositions and methods for spatial analysis of multicell suspensions |
| WO2026068813A1 (en) | 2024-09-30 | 2026-04-02 | Resolve Biosciences Gmbh | Immuno-oncology gene panel compositions and methods of making and use thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019113547A1 (en) | 2017-12-08 | 2019-06-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes |
| JP2019518434A (ja) | 2016-04-25 | 2019-07-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ｉｎ ｓｉｔｕ分子検出のためのハイブリダイゼーション連鎖反応法 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1228811A (en) | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Assay method utilizing polynucleotide sequences |
| US6734401B2 (en) | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| WO2003016574A1 (en) | 2001-08-16 | 2003-02-27 | Zhifeng Shao | Analysis of gene expression profiles using sequential hybridization |
| CA2480673A1 (en) | 2002-03-29 | 2003-10-09 | Genentech, Inc. | Methods and compositions for detection and quantitation of nucleic acid analytes |
| DK2500439T4 (da) | 2005-06-20 | 2017-11-13 | Advanced Cell Diagnostics Inc | Kits og produkter til detektering af nukleinsyrer i individuelle celler og til identifikation af sjældne celler fra store heterogene cellepopulationer |
| WO2011038403A1 (en) | 2009-09-28 | 2011-03-31 | Yuling Luo | Methods of detecting nucleic acid sequences with high specificity |
| US9677125B2 (en) | 2009-10-21 | 2017-06-13 | General Electric Company | Detection of plurality of targets in biological samples |
| WO2011112634A2 (en) | 2010-03-08 | 2011-09-15 | California Institute Of Technology | Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells |
| CN101895587B (zh) | 2010-07-06 | 2015-09-16 | 中兴通讯股份有限公司 | 防止用户私自修改ip地址的方法、装置和系统 |
| EP4249605B1 (en) | 2011-12-22 | 2024-08-28 | President And Fellows Of Harvard College | Methods for analyte detection |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| WO2013157821A1 (en) | 2012-04-19 | 2013-10-24 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage |
| US20160369329A1 (en) | 2013-04-30 | 2016-12-22 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers |
| US10510435B2 (en) * | 2013-04-30 | 2019-12-17 | California Institute Of Technology | Error correction of multiplex imaging analysis by sequential hybridization |
| CA2907493A1 (en) | 2013-04-30 | 2014-11-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding |
| US20150105298A1 (en) | 2013-10-10 | 2015-04-16 | The Research Foundation For The State University Of New York | Multi-oligomer in situ hybridization probes |
| EP3169810B1 (en) | 2014-07-17 | 2024-09-04 | California Institute of Technology | Multiplex analysis of molecules in single cells by image correlation |
| WO2016081740A1 (en) | 2014-11-21 | 2016-05-26 | Nanostring Technologies, Inc. | Enzyme- and amplification-free sequencing |
| EP3307908B1 (en) | 2015-06-09 | 2019-09-11 | Life Technologies Corporation | Methods for molecular tagging |
| CN116200462B (zh) | 2016-05-16 | 2025-10-03 | 布鲁克空间生物学公司 | 用于检测样品中目标核酸的方法 |
| CN109689661B (zh) | 2016-06-28 | 2021-10-15 | 百时美施贵宝公司 | 髓过氧化物酶的大环抑制剂 |
| WO2018026873A1 (en) * | 2016-08-01 | 2018-02-08 | California Institute Of Technology | Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism |
| CA3034617A1 (en) | 2016-08-30 | 2018-03-08 | California Institute Of Technology | Immunohistochemistry via hybridization chain reaction |
| EP4556575A3 (en) | 2016-11-21 | 2025-10-08 | Bruker Spatial Biology, Inc. | A method for sequencing nucleic acids |
| EP3794146B1 (en) | 2018-05-14 | 2025-12-10 | Bruker Spatial Biology, Inc. | Method for identifying a predetermined nucleotide sequence |
| WO2020093019A2 (en) | 2018-11-01 | 2020-05-07 | California Institute Of Technology | A multi-channel line scanner for fast large field and confocal imaging |
| WO2020117713A1 (en) | 2018-12-03 | 2020-06-11 | California Institute Of Technology | In situ readout of dna barcodes |
| EP3894583A4 (en) | 2018-12-13 | 2022-11-09 | President and Fellows of Harvard College | AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS |
| WO2020163397A2 (en) | 2019-02-04 | 2020-08-13 | Akoya Biosciences, Inc. | Analyte detection by selective labeling of biological samples |
| CN119220641A (zh) | 2019-05-31 | 2024-12-31 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| KR20220035958A (ko) | 2019-07-30 | 2022-03-22 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 표적 분석에 관한 방법 및 조성물 |
| CN114729391A (zh) | 2019-09-30 | 2022-07-08 | 阿科亚生物科学股份有限公司 | 用酶介导的放大的多路复用成像 |
| EP4065728A1 (en) | 2019-11-25 | 2022-10-05 | California Institute of Technology | Quantitative analysis of single-cell proteins by identification and quantification separation (duet) |
| US20230340457A1 (en) | 2019-11-26 | 2023-10-26 | President And Fellows Of Harvard College | Compositions, sets, and methods related to target analysis |
| EP4073246A4 (en) | 2019-12-12 | 2023-12-06 | President And Fellows Of Harvard College | COMPOSITIONS AND METHODS FOR LIGHT-DIRECTED BIOMOLECULAR BARCODING |
| EP4158058B1 (en) | 2020-06-02 | 2025-08-06 | 10X Genomics, Inc. | Enrichment of nucleic acid sequences |
| US12492427B2 (en) * | 2020-06-18 | 2025-12-09 | Resolve Biosciences Gmbh | Multiplex method for detecting different analytes in a sample |
| WO2021258024A1 (en) | 2020-06-19 | 2021-12-23 | California Institute Of Technology | Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing |
-
2021
- 2021-06-18 US US18/001,933 patent/US12492427B2/en active Active
- 2021-06-18 WO PCT/EP2021/066620 patent/WO2021255244A1/en not_active Ceased
- 2021-06-18 CN CN202180041893.2A patent/CN115702249A/zh active Pending
- 2021-06-18 JP JP2022575771A patent/JP7828901B2/ja active Active
- 2021-06-18 AU AU2021294274A patent/AU2021294274A1/en active Pending
- 2021-06-18 CA CA3181737A patent/CA3181737A1/en active Pending
- 2021-06-18 JP JP2022575762A patent/JP2023529682A/ja active Pending
- 2021-06-18 KR KR1020227043397A patent/KR20230023646A/ko active Pending
- 2021-06-18 EP EP21732086.0A patent/EP4168575A1/en active Pending
- 2021-06-18 CA CA3181725A patent/CA3181725A1/en active Pending
- 2021-06-18 EP EP21733989.4A patent/EP4168576A2/en active Pending
- 2021-06-18 AU AU2021291051A patent/AU2021291051A1/en active Pending
- 2021-06-18 CN CN202180041889.6A patent/CN115698322A/zh active Pending
- 2021-06-18 WO PCT/EP2021/066668 patent/WO2021255263A2/en not_active Ceased
- 2021-06-18 US US18/001,930 patent/US20240279736A1/en active Pending
- 2021-06-18 KR KR1020227043398A patent/KR20230023647A/ko active Pending
- 2021-12-17 US US17/554,093 patent/US12442037B2/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019518434A (ja) | 2016-04-25 | 2019-07-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ｉｎ ｓｉｔｕ分子検出のためのハイブリダイゼーション連鎖反応法 |
| WO2019113547A1 (en) | 2017-12-08 | 2019-06-13 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding with rapid switching and rehybridzation of probes |
Non-Patent Citations (1)
| Title |
|---|
| J.C.W. Hildyard et al.，Multiplex in situ hybridization within a single transcript: RNAscope reveals dystrophin mRNA dynamics，bioRxiv [online]，2019年10月03日，[検索日2025.04.17], インターネット<URL: https://www.biorxiv.org/content/10.1101/791780v1> |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20230023646A (ko) | 2023-02-17 |
| AU2021294274A1 (en) | 2022-12-08 |
| JP2023530889A (ja) | 2023-07-20 |
| EP4168575A1 (en) | 2023-04-26 |
| WO2021255244A1 (en) | 2021-12-23 |
| US20220205028A1 (en) | 2022-06-30 |
| CN115698322A (zh) | 2023-02-03 |
| US20230227907A1 (en) | 2023-07-20 |
| CA3181725A1 (en) | 2021-12-23 |
| WO2021255263A2 (en) | 2021-12-23 |
| WO2021255263A3 (en) | 2022-02-03 |
| KR20230023647A (ko) | 2023-02-17 |
| US12442037B2 (en) | 2025-10-14 |
| JP2023529682A (ja) | 2023-07-11 |
| US20240279736A1 (en) | 2024-08-22 |
| EP4168576A2 (en) | 2023-04-26 |
| AU2021291051A1 (en) | 2022-12-08 |
| US12492427B2 (en) | 2025-12-09 |
| CN115702249A (zh) | 2023-02-14 |
| CA3181737A1 (en) | 2021-12-23 |
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