JP7749577B2 - 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物 - Google Patents

非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物

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Publication number
JP7749577B2
JP7749577B2 JP2022558098A JP2022558098A JP7749577B2 JP 7749577 B2 JP7749577 B2 JP 7749577B2 JP 2022558098 A JP2022558098 A JP 2022558098A JP 2022558098 A JP2022558098 A JP 2022558098A JP 7749577 B2 JP7749577 B2 JP 7749577B2
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ehgt
syfp2
sequence
cells
expression
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JPWO2021195591A5 (https=
JP2023518980A (ja
JP2023518980A5 (https=
Inventor
ティン，ジョナサン
タシッチ，ボシリカ
リーバイ，ボアズ・ピー
デイグル，ターニャ
グレイバック，ルーカス・ティー
レイン，エドワード・セバスチャン
ミッチ，ジョン・ケイ
セデノ・コルテス，アドリアナ・エステラ
ゼン，ホンクイ
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アレン インスティテュート
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Priority to JP2025157795A priority Critical patent/JP2026001073A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2022558098A 2020-03-27 2021-03-26 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物 Active JP7749577B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025157795A JP2026001073A (ja) 2020-03-27 2025-09-24 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063001159P 2020-03-27 2020-03-27
US63/001,159 2020-03-27
PCT/US2021/024525 WO2021195591A2 (en) 2020-03-27 2021-03-26 Artificial expression constructs for selectively modulating gene expression in non-neuronal brain cells

Related Child Applications (1)

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JP2025157795A Division JP2026001073A (ja) 2020-03-27 2025-09-24 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物

Publications (4)

Publication Number Publication Date
JP2023518980A JP2023518980A (ja) 2023-05-09
JPWO2021195591A5 JPWO2021195591A5 (https=) 2024-02-27
JP2023518980A5 JP2023518980A5 (https=) 2024-02-27
JP7749577B2 true JP7749577B2 (ja) 2025-10-06

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JP2022558098A Active JP7749577B2 (ja) 2020-03-27 2021-03-26 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物
JP2025157795A Pending JP2026001073A (ja) 2020-03-27 2025-09-24 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物

Family Applications After (1)

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JP2025157795A Pending JP2026001073A (ja) 2020-03-27 2025-09-24 非神経脳細胞での遺伝子発現を選択的に調節する人工発現構築物

Country Status (6)

Country Link
US (1) US20230117172A1 (https=)
EP (1) EP4127182A4 (https=)
JP (2) JP7749577B2 (https=)
AU (1) AU2021242353A1 (https=)
CA (1) CA3173609A1 (https=)
WO (1) WO2021195591A2 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3207224A1 (en) * 2021-02-02 2022-08-11 Bryan GORE Artificial expression constructs for modulating gene expression in gabaergic neurons and astrocytes
WO2023060112A1 (en) * 2021-10-05 2023-04-13 Allen Institute Artificial expression constructs for modulating gene expression in the cerebellum and a secondary cell type
JP2025520385A (ja) * 2022-06-13 2025-07-03 アレン インスティテュート 中枢神経系の非神経細胞において遺伝子発現を調節するための人工発現コンストラクト

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020151066A1 (en) 2000-07-14 2002-10-17 Rubenstein John L. Production of gabaergic cells
US20060127358A1 (en) 2002-05-01 2006-06-15 Nicholas Muzyczka Raav expression systems and methods for enhancing transduction of mammalian neural cells
US20190247516A1 (en) 2017-11-10 2019-08-15 Norwegian University Of Science And Technology (Ntnu) Neuronal enhancers

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080039413A1 (en) * 2003-10-21 2008-02-14 Morris David W Novel compositions and methods in cancer
EP4154903A1 (en) * 2008-04-22 2023-03-29 Vib Vzw Liver-specific nucleic acid regulatory elements and methods and use thereof
WO2013155222A2 (en) * 2012-04-10 2013-10-17 The Regents Of The University Of California Brain-specific enhancers for cell-based therapy
CN110546257B (zh) * 2017-03-17 2024-03-01 阿德夫拉姆生物技术股份有限公司 用于增强基因表达的组合物和方法
WO2019068854A1 (en) * 2017-10-06 2019-04-11 Ospedale San Raffaele S.R.L. GENE THERAPY OF NEURODEGENERATIVE DISEASES USING VAA VECTORS
AU2019253700B2 (en) * 2018-04-09 2025-08-14 Allen Institute Rescuing voltage-gated sodium channel function in inhibitory neurons

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020151066A1 (en) 2000-07-14 2002-10-17 Rubenstein John L. Production of gabaergic cells
US20060127358A1 (en) 2002-05-01 2006-06-15 Nicholas Muzyczka Raav expression systems and methods for enhancing transduction of mammalian neural cells
US20190247516A1 (en) 2017-11-10 2019-08-15 Norwegian University Of Science And Technology (Ntnu) Neuronal enhancers

Also Published As

Publication number Publication date
JP2023518980A (ja) 2023-05-09
CA3173609A1 (en) 2021-09-30
EP4127182A4 (en) 2024-07-31
WO2021195591A2 (en) 2021-09-30
AU2021242353A1 (en) 2022-11-03
US20230117172A1 (en) 2023-04-20
JP2026001073A (ja) 2026-01-06
EP4127182A2 (en) 2023-02-08
WO2021195591A3 (en) 2021-11-25

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