JP7730563B2 - ブタ肺胞マクロファージ不死化培養細胞株、ブタ肺胞マクロファージ不死化培養細胞株の製造方法、ブタ肺胞マクロファージ不死化培養細胞株の調製用試薬及びワクチンの製造方法 - Google Patents

ブタ肺胞マクロファージ不死化培養細胞株、ブタ肺胞マクロファージ不死化培養細胞株の製造方法、ブタ肺胞マクロファージ不死化培養細胞株の調製用試薬及びワクチンの製造方法

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JP7730563B2
JP7730563B2 JP2022538023A JP2022538023A JP7730563B2 JP 7730563 B2 JP7730563 B2 JP 7730563B2 JP 2022538023 A JP2022538023 A JP 2022538023A JP 2022538023 A JP2022538023 A JP 2022538023A JP 7730563 B2 JP7730563 B2 JP 7730563B2
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cell line
immortalized
pam
cultured
alveolar macrophage
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JPWO2022019306A5 (https=
JPWO2022019306A1 (https=
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真 小澤
結子 菅谷
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Kagoshima University NUC
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Kagoshima University NUC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0006—Modification of the membrane of cells, e.g. cell decoration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2022538023A 2020-07-22 2021-07-20 ブタ肺胞マクロファージ不死化培養細胞株、ブタ肺胞マクロファージ不死化培養細胞株の製造方法、ブタ肺胞マクロファージ不死化培養細胞株の調製用試薬及びワクチンの製造方法 Active JP7730563B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020125310 2020-07-22
JP2020125310 2020-07-22
PCT/JP2021/027164 WO2022019306A1 (ja) 2020-07-22 2021-07-20 ブタ肺胞マクロファージ不死化培養細胞株、ブタ肺胞マクロファージ不死化培養細胞株の製造方法、ブタ肺胞マクロファージ不死化培養細胞株の調製用試薬及びワクチンの製造方法

Publications (3)

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JPWO2022019306A1 JPWO2022019306A1 (https=) 2022-01-27
JPWO2022019306A5 JPWO2022019306A5 (https=) 2023-06-13
JP7730563B2 true JP7730563B2 (ja) 2025-08-28

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117487761A (zh) * 2023-09-25 2024-02-02 南京农业大学 表达Cre基因猪骨髓巨噬细胞系的构建方法及其应用
WO2025182870A1 (ja) * 2024-02-26 2025-09-04 国立研究開発法人農業・食品産業技術総合研究機構 不死化カワイノシシマクロファージ
CN118389443A (zh) * 2024-04-25 2024-07-26 上海交通大学 猪GSDMD敲除细胞系的构建方法及在PDCoV感染中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017500029A (ja) 2013-12-12 2017-01-05 インターベット インターナショナル ベー． フェー． 不死化ブタ肺胞マクロファージ
CN109517797A (zh) 2018-07-25 2019-03-26 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 表达猪繁殖障碍与呼吸综合症病毒受体cd163的猪永生化肺泡巨噬细胞细胞系及其应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017500029A (ja) 2013-12-12 2017-01-05 インターベット インターナショナル ベー． フェー． 不死化ブタ肺胞マクロファージ
CN109517797A (zh) 2018-07-25 2019-03-26 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 表达猪繁殖障碍与呼吸综合症病毒受体cd163的猪永生化肺泡巨噬细胞细胞系及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Dev. Comp. Immunol.，2018年，Vol. 79，p. 51-58
J. Virol. Methods，2002年，Vol. 104，pp. 203-216
Oncotarget，2018年，Vol. 9, No. 15，pp. 12174-12185

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JPWO2022019306A1 (https=) 2022-01-27

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