JP7601339B2 - Ａａｖを産生する方法 - Google Patents

Ａａｖを産生する方法 Download PDF

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JP7601339B2
JP7601339B2 JP2022569478A JP2022569478A JP7601339B2 JP 7601339 B2 JP7601339 B2 JP 7601339B2 JP 2022569478 A JP2022569478 A JP 2022569478A JP 2022569478 A JP2022569478 A JP 2022569478A JP 7601339 B2 JP7601339 B2 JP 7601339B2
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aav
perfusion
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cell
production
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JP2023518919A (ja
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ジュリアナ コロネル
ジルケ ヴィッシンク
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ツェヴェック ファーマシューティカルズ ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022569478A 2020-11-25 2021-11-23 Ａａｖを産生する方法 Active JP7601339B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024111946A JP2024144435A (ja) 2020-11-25 2024-07-11 Ａａｖを産生する方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20209811.7 2020-11-25
EP20209811.7A EP4006141A1 (en) 2020-11-25 2020-11-25 Method for the production of aav
PCT/EP2021/082615 WO2022112218A1 (en) 2020-11-25 2021-11-23 Method for the production of aav

Related Child Applications (1)

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JP2024111946A Division JP2024144435A (ja) 2020-11-25 2024-07-11 Ａａｖを産生する方法

Publications (2)

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JP2023518919A JP2023518919A (ja) 2023-05-08
JP7601339B2 true JP7601339B2 (ja) 2024-12-17

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JP2022569478A Active JP7601339B2 (ja) 2020-11-25 2021-11-23 Ａａｖを産生する方法
JP2024111946A Pending JP2024144435A (ja) 2020-11-25 2024-07-11 Ａａｖを産生する方法

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JP2024111946A Pending JP2024144435A (ja) 2020-11-25 2024-07-11 Ａａｖを産生する方法

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US (1) US20240263148A1 (enExample)
EP (3) EP4006141A1 (enExample)
JP (2) JP7601339B2 (enExample)
KR (1) KR102700433B1 (enExample)
CN (1) CN116209748A (enExample)
AU (1) AU2021388811B2 (enExample)
CA (1) CA3174017C (enExample)
DK (1) DK4107252T3 (enExample)
ES (1) ES3017247T3 (enExample)
FI (1) FI4107252T3 (enExample)
IL (1) IL296770B2 (enExample)
MX (1) MX2022013385A (enExample)
PT (1) PT4107252T (enExample)
WO (1) WO2022112218A1 (enExample)
ZA (1) ZA202210745B (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202323529A (zh) * 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
TW202342740A (zh) * 2022-03-07 2023-11-01 美商奧崔基尼克斯製藥公司 改良的批量aav生產系統和方法
JP2025519228A (ja) * 2022-06-03 2025-06-24 エフ． ホフマン－ラ ロシュ アーゲー 組換えａａｖ粒子を生成するための方法
WO2025047766A1 (ja) * 2023-08-29 2025-03-06 富士フイルム株式会社 ウイルスの製造方法および細胞への核酸導入方法
WO2025171001A1 (en) 2024-02-06 2025-08-14 Regeneron Pharmaceuticals, Inc. Compositions and methods for improved production of aav viral vectors
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
EP4606816A1 (en) 2024-02-26 2025-08-27 Universitat Autónoma De Barcelona (UAB) Method for production of gene therapy vehicles in mammalian cells
WO2025226558A1 (en) 2024-04-22 2025-10-30 Ultragenyx Pharmaceutical Inc. Multi-step filtration and clarification methods for recombinant adeno-associated virus purification
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127582A1 (en) 1997-09-05 2002-09-12 Atkinson Edward M. Methods for generating high titer helper-free preparations of released recombinant AAV vectors
WO2020154607A1 (en) 2019-01-25 2020-07-30 Biogen Ma Inc. Seed culture process for aav production
WO2020223274A1 (en) 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020168342A1 (en) * 1994-11-03 2002-11-14 Cell Genesys, Inc. Novel adenoviral vectors, packaging cell lines, recombinant adenoviruses and methods
WO2000024916A1 (en) * 1998-10-27 2000-05-04 Crucell Holland B.V. Improved aav vector production
US6844192B2 (en) * 2001-06-29 2005-01-18 Wake Forest University Adenovirus E4 protein variants for virus production
CA3018264A1 (en) * 2016-04-12 2017-10-19 Artemis Biosystems, Inc. Perfusion filtration systems
EP3456822A1 (en) * 2017-09-19 2019-03-20 CEVEC Pharmaceuticals GmbH Inducible aav rep genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127582A1 (en) 1997-09-05 2002-09-12 Atkinson Edward M. Methods for generating high titer helper-free preparations of released recombinant AAV vectors
WO2020154607A1 (en) 2019-01-25 2020-07-30 Biogen Ma Inc. Seed culture process for aav production
WO2020223274A1 (en) 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Molecular Therapy，2016年02月，Vol.24, No.2，pp.287-297，https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817810/のPDFファイルを参照
ウイルス，2007年，第５７巻第１号，pp.47-56，http://jsv.umin.jp/journal/v57-1pdf/virus57-1_047-056.pdf参照

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Publication number Publication date
US20240263148A1 (en) 2024-08-08
ES3017247T3 (en) 2025-05-12
IL296770B2 (en) 2025-10-01
EP4107252A1 (en) 2022-12-28
EP4107252B1 (en) 2025-02-19
CA3174017A1 (en) 2022-06-02
AU2021388811A1 (en) 2022-11-03
AU2021388811B2 (en) 2023-02-23
MX2022013385A (es) 2022-11-30
EP4006141A1 (en) 2022-06-01
PT4107252T (pt) 2025-03-14
CA3174017C (en) 2025-05-06
WO2022112218A1 (en) 2022-06-02
CN116209748A (zh) 2023-06-02
JP2024144435A (ja) 2024-10-11
EP4596674A2 (en) 2025-08-06
KR20220164801A (ko) 2022-12-13
FI4107252T3 (fi) 2025-03-26
KR102700433B1 (ko) 2024-08-30
DK4107252T3 (da) 2025-03-17
IL296770A (en) 2022-11-01
ZA202210745B (en) 2023-05-31
IL296770B1 (en) 2025-06-01
JP2023518919A (ja) 2023-05-08
EP4596674A3 (en) 2025-10-22

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