JP7579817B2 - Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 - Google Patents
Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 Download PDFInfo
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- JP7579817B2 JP7579817B2 JP2021577412A JP2021577412A JP7579817B2 JP 7579817 B2 JP7579817 B2 JP 7579817B2 JP 2021577412 A JP2021577412 A JP 2021577412A JP 2021577412 A JP2021577412 A JP 2021577412A JP 7579817 B2 JP7579817 B2 JP 7579817B2
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- C12Q1/6844—Nucleic acid amplification reactions
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- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
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- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
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- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
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- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024188978A JP2025014002A (ja) | 2019-07-03 | 2024-10-28 | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962870471P | 2019-07-03 | 2019-07-03 | |
| US201962870426P | 2019-07-03 | 2019-07-03 | |
| US62/870,426 | 2019-07-03 | ||
| US62/870,471 | 2019-07-03 | ||
| US201962952603P | 2019-12-23 | 2019-12-23 | |
| US201962952598P | 2019-12-23 | 2019-12-23 | |
| US62/952,598 | 2019-12-23 | ||
| US62/952,603 | 2019-12-23 | ||
| PCT/US2020/040621 WO2021003343A1 (en) | 2019-07-03 | 2020-07-02 | Identification, characterization, and quantitation of crispr-introduced double-stranded dna break repairs |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024188978A Division JP2025014002A (ja) | 2019-07-03 | 2024-10-28 | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022540792A JP2022540792A (ja) | 2022-09-20 |
| JP2022540792A5 JP2022540792A5 (https=) | 2023-04-18 |
| JP7579817B2 true JP7579817B2 (ja) | 2024-11-08 |
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ID=74065411
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021577412A Active JP7579817B2 (ja) | 2019-07-03 | 2020-07-02 | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
| JP2024188978A Pending JP2025014002A (ja) | 2019-07-03 | 2024-10-28 | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024188978A Pending JP2025014002A (ja) | 2019-07-03 | 2024-10-28 | Crisprで導入された二本鎖dna切断修復の同定、特徴付けおよび定量化 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US12254959B2 (https=) |
| EP (1) | EP3994258A4 (https=) |
| JP (2) | JP7579817B2 (https=) |
| AU (1) | AU2020301025A1 (https=) |
| CA (1) | CA3144106A1 (https=) |
| IL (1) | IL289421A (https=) |
| WO (1) | WO2021003343A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119193713A (zh) | 2019-10-24 | 2024-12-27 | 合成Dna技术公司 | 修饰的双链供体模板 |
| CN119343462A (zh) | 2022-05-13 | 2025-01-21 | 合成Dna技术公司 | 使用独特分子标识符改善长读测序的准确性和crispr编辑的表征 |
| WO2026039499A1 (en) | 2024-08-14 | 2026-02-19 | Integrated Dna Technologies, Inc. | Adaptered-tag blocking oligonucleotides |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170053062A1 (en) | 2014-01-27 | 2017-02-23 | Georgia Tech Research Corporation | Methods and systems for identifying crispr/cas off-target sites |
| US20170211142A1 (en) | 2015-10-22 | 2017-07-27 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| US20180068062A1 (en) | 2016-08-17 | 2018-03-08 | The Broad Institute, Inc. | Methods for identifying novel gene editing elements |
| US20180163265A1 (en) | 2014-12-19 | 2018-06-14 | The Broad Institute Inc. | Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing |
| JP2018518183A (ja) | 2015-06-18 | 2018-07-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | 新規crispr酵素及び系 |
| JP2019504646A (ja) | 2016-02-11 | 2019-02-21 | ホライズン・ディスカバリー・リミテッド | 複製的トランスポゾン系 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9809904B2 (en) | 2011-04-21 | 2017-11-07 | University Of Washington Through Its Center For Commercialization | Methods for retrieval of sequence-verified DNA constructs |
| CN111254500B (zh) | 2012-12-10 | 2024-01-23 | 分析生物科学有限公司 | 靶向基因组分析的方法 |
| CA2906365A1 (en) | 2013-03-15 | 2014-09-18 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| US11306328B2 (en) | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
| CN106604994B (zh) | 2014-06-23 | 2021-12-14 | 通用医疗公司 | 通过测序评估的DSBs的全基因组无偏鉴定(GUIDE-Seq) |
| WO2016030899A1 (en) | 2014-08-28 | 2016-03-03 | Yeda Research And Development Co. Ltd. | Methods of treating amyotrophic lateral scleroses |
| EP3221813A4 (en) | 2014-11-20 | 2018-04-18 | Children's Medical Center Corporation | Methods relating to the detection of recurrent and non-specific double strand breaks in the genome |
| WO2016138500A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| JP2018523991A (ja) | 2015-06-22 | 2018-08-30 | マクマスター・ユニバーシティMcmaster University | 構造スイッチングのタンデム反応、核酸分解消化および核酸アセンブリーの増幅を含むバイオセンサー |
| WO2017040511A1 (en) | 2015-08-31 | 2017-03-09 | Agilent Technologies, Inc. | Compounds and methods for crispr/cas-based genome editing by homologous recombination |
| US20170081679A1 (en) | 2015-09-22 | 2017-03-23 | Jie Xu | Methods of improving nuclease mediated homologous recombination |
| EP4144844B1 (en) | 2015-10-12 | 2025-09-10 | DuPont US Holding, LLC | Protected dna templates for gene modification and increased homologous recombination in cells and methods of use |
| CN116064748A (zh) | 2015-11-25 | 2023-05-05 | 综合基因技术公司 | 用于变体检测的方法 |
| ES2962711T3 (es) | 2016-12-20 | 2024-03-20 | Bristol Myers Squibb Co | Métodos para aumentar la eficacia de la reparación dirigida por homología (HDR) en el genoma celular |
| US11981898B2 (en) | 2017-06-16 | 2024-05-14 | Applied Stemcell, Inc. | Gene editing methods with increased knock-in efficiency |
| US20190062736A1 (en) * | 2017-08-22 | 2019-02-28 | Board Of Regents, The University Of Texas System | In situ and in vivo analysis of chromatin interactions by biotinylated dcas9 protein |
| EP3679143A1 (en) | 2017-09-08 | 2020-07-15 | Life Technologies Corporation | Methods for improved homologous recombination and compositions thereof |
| WO2019110067A1 (en) | 2017-12-07 | 2019-06-13 | Aarhus Universitet | Hybrid nanoparticle |
| US12406749B2 (en) * | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| BR112020018902A2 (pt) | 2018-03-20 | 2021-01-26 | Tokyo Institute Of Technology | oligonucleotídeo antissenso com toxicidade reduzida |
| WO2019246553A1 (en) * | 2018-06-21 | 2019-12-26 | Memorial Sloan Kettering Cancer Center | Compositions & methods for monitoring dna repair |
| EP3935185A1 (en) | 2019-03-04 | 2022-01-12 | King Abdullah University Of Science And Technology | Compositions and methods of labeling nucleic acids and sequencing and analysis thereof |
| CN119193713A (zh) | 2019-10-24 | 2024-12-27 | 合成Dna技术公司 | 修饰的双链供体模板 |
-
2020
- 2020-07-02 CA CA3144106A patent/CA3144106A1/en active Pending
- 2020-07-02 AU AU2020301025A patent/AU2020301025A1/en active Pending
- 2020-07-02 EP EP20835024.9A patent/EP3994258A4/en active Pending
- 2020-07-02 US US16/919,577 patent/US12254959B2/en active Active
- 2020-07-02 JP JP2021577412A patent/JP7579817B2/ja active Active
- 2020-07-02 WO PCT/US2020/040621 patent/WO2021003343A1/en not_active Ceased
-
2021
- 2021-12-27 IL IL289421A patent/IL289421A/en unknown
-
2024
- 2024-10-28 JP JP2024188978A patent/JP2025014002A/ja active Pending
-
2025
- 2025-02-12 US US19/052,059 patent/US20250218536A1/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170053062A1 (en) | 2014-01-27 | 2017-02-23 | Georgia Tech Research Corporation | Methods and systems for identifying crispr/cas off-target sites |
| US20180163265A1 (en) | 2014-12-19 | 2018-06-14 | The Broad Institute Inc. | Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing |
| JP2018518183A (ja) | 2015-06-18 | 2018-07-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | 新規crispr酵素及び系 |
| US20170211142A1 (en) | 2015-10-22 | 2017-07-27 | The Broad Institute, Inc. | Novel crispr enzymes and systems |
| JP2019504646A (ja) | 2016-02-11 | 2019-02-21 | ホライズン・ディスカバリー・リミテッド | 複製的トランスポゾン系 |
| US20180068062A1 (en) | 2016-08-17 | 2018-03-08 | The Broad Institute, Inc. | Methods for identifying novel gene editing elements |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3994258A4 (en) | 2022-10-26 |
| WO2021003343A1 (en) | 2021-01-07 |
| CA3144106A1 (en) | 2021-01-07 |
| AU2020301025A1 (en) | 2022-01-20 |
| US20250218536A1 (en) | 2025-07-03 |
| JP2022540792A (ja) | 2022-09-20 |
| US12254959B2 (en) | 2025-03-18 |
| JP2025014002A (ja) | 2025-01-28 |
| EP3994258A1 (en) | 2022-05-11 |
| US20210002700A1 (en) | 2021-01-07 |
| IL289421A (en) | 2022-02-01 |
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