JP7566216B1 - 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム - Google Patents

分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム Download PDF

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JP7566216B1
JP7566216B1 JP2024537453A JP2024537453A JP7566216B1 JP 7566216 B1 JP7566216 B1 JP 7566216B1 JP 2024537453 A JP2024537453 A JP 2024537453A JP 2024537453 A JP2024537453 A JP 2024537453A JP 7566216 B1 JP7566216 B1 JP 7566216B1
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image
fluorescence
separation
fluorescent
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JPWO2024176615A5 (https=
JPWO2024176615A1 (https=
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貴文 樋口
賢一郎 池村
航 加茂
なつみ 加藤
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Hamamatsu Photonics KK
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2024537453A 2023-02-24 2023-12-26 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム Active JP7566216B1 (ja)

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PCT/JP2023/046652 WO2024176615A1 (ja) 2023-02-24 2023-12-26 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム

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JP7566216B1 true JP7566216B1 (ja) 2024-10-11
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JP2024172416A Pending JP2024174139A (ja) 2023-02-24 2024-10-01 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム

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EP (1) EP4653848A1 (https=)
JP (2) JP7566216B1 (https=)
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WO (1) WO2024176615A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026036380A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 生成方法、生成装置、及びプログラム

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005036143A1 (ja) * 2003-10-10 2005-04-21 Hamamatsu Photonics K.K. 蛍光色素の濃度を定量する方法およびシステム
JP2008541179A (ja) * 2005-05-18 2008-11-20 オリンパス・ソフト・イメージング・ソリューションズ・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング 多色画像、特に顕微鏡多色透過画像におけるスペクトル重畳またはカラー重畳画像寄与の分離
US20210208076A1 (en) * 2020-01-03 2021-07-08 Korea Advanced Institute Of Science And Technology Method and Apparatus for Multiplexed Imaging of Spectrally-Similar Fluorophores
WO2023026742A1 (ja) * 2021-08-25 2023-03-02 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005036143A1 (ja) * 2003-10-10 2005-04-21 Hamamatsu Photonics K.K. 蛍光色素の濃度を定量する方法およびシステム
JP2008541179A (ja) * 2005-05-18 2008-11-20 オリンパス・ソフト・イメージング・ソリューションズ・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング 多色画像、特に顕微鏡多色透過画像におけるスペクトル重畳またはカラー重畳画像寄与の分離
US20210208076A1 (en) * 2020-01-03 2021-07-08 Korea Advanced Institute Of Science And Technology Method and Apparatus for Multiplexed Imaging of Spectrally-Similar Fluorophores
WO2023026742A1 (ja) * 2021-08-25 2023-03-02 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

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CN120752513A (zh) 2025-10-03
JP2024174139A (ja) 2024-12-13
JPWO2024176615A1 (https=) 2024-08-29
WO2024176615A1 (ja) 2024-08-29

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