JP7548305B2 - 切断可能なｄｎａコード化ライブラリ - Google Patents

切断可能なｄｎａコード化ライブラリ Download PDF

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JP7548305B2
JP7548305B2 JP2022526549A JP2022526549A JP7548305B2 JP 7548305 B2 JP7548305 B2 JP 7548305B2 JP 2022526549 A JP2022526549 A JP 2022526549A JP 2022526549 A JP2022526549 A JP 2022526549A JP 7548305 B2 JP7548305 B2 JP 7548305B2
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group
compound according
compound
oligonucleotide
nucleic acid
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JPWO2021241528A1 (https=
JPWO2021241528A5 (https=
Inventor
雅俊 丹羽
宗史 ▲徳▼川
潤 林田
一 深野
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Nissan Chemical Corp
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Nissan Chemical Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Structural Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022526549A 2020-05-25 2021-05-24 切断可能なｄｎａコード化ライブラリ Active JP7548305B2 (ja)

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JP2024146187A JP7838610B2 (ja) 2020-05-25 2024-08-28 切断可能なｄｎａコード化ライブラリ

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JP2020090304 2020-05-25
JP2020090304 2020-05-25
PCT/JP2021/019685 WO2021241528A1 (ja) 2020-05-25 2021-05-24 切断可能なｄｎａコード化ライブラリ

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JPWO2021241528A1 JPWO2021241528A1 (https=) 2021-12-02
JPWO2021241528A5 JPWO2021241528A5 (https=) 2024-06-03
JP7548305B2 true JP7548305B2 (ja) 2024-09-10

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US (1) US20230212214A1 (https=)
EP (1) EP4159766A4 (https=)
JP (2) JP7548305B2 (https=)
KR (1) KR20230015347A (https=)
CN (1) CN115461456A (https=)
CA (1) CA3185063A1 (https=)
TW (1) TWI905196B (https=)
WO (1) WO2021241528A1 (https=)

Families Citing this family (2)

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Publication number Priority date Publication date Assignee Title
WO2024259563A1 (zh) * 2023-06-19 2024-12-26 深圳华大智造科技股份有限公司 一种多重rt-pcr扩增方法及其应用
WO2025053257A1 (ja) * 2023-09-07 2025-03-13 旭化成ファーマ株式会社 オリゴヌクレオチドによりコード化された化合物の製造方法、およびその応用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006515752A (ja) 2002-10-30 2006-06-08 ヌエヴォリューション・アクティーゼルスカブ 二官能性複合体の合成方法
JP2007524662A (ja) 2003-12-17 2007-08-30 プラエシス ファーマシューティカルズ インコーポレーテッド コードされたライブラリーの合成のための方法
JP2012517812A (ja) 2009-02-13 2012-08-09 エックス−ケム インコーポレイテッド Ｄｎａコード化ライブラリを作製およびスクリーニングする方法
WO2018143475A1 (ja) 2017-02-06 2018-08-09 日産化学工業株式会社 一本鎖オリゴヌクレオチド
WO2019060857A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES
JP2020510403A (ja) 2017-03-17 2020-04-09 成都先導薬物開発股▲フン▼有限公司Ｈｉｔｇｅｎ Ｉｎｃ． エンコードライブラリの合成方法及び組成物

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9206791D0 (en) 1992-03-27 1992-05-13 Exxon Chemical Patents Inc Improved leather
WO2011056185A2 (en) 2009-11-04 2011-05-12 President And Fellows Of Harvard College Reactivity-dependent and interaction-dependent pcr
SG10201907347YA (en) * 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
WO2017218293A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006515752A (ja) 2002-10-30 2006-06-08 ヌエヴォリューション・アクティーゼルスカブ 二官能性複合体の合成方法
JP2007524662A (ja) 2003-12-17 2007-08-30 プラエシス ファーマシューティカルズ インコーポレーテッド コードされたライブラリーの合成のための方法
JP2012517812A (ja) 2009-02-13 2012-08-09 エックス−ケム インコーポレイテッド Ｄｎａコード化ライブラリを作製およびスクリーニングする方法
WO2018143475A1 (ja) 2017-02-06 2018-08-09 日産化学工業株式会社 一本鎖オリゴヌクレオチド
JP2020510403A (ja) 2017-03-17 2020-04-09 成都先導薬物開発股▲フン▼有限公司Ｈｉｔｇｅｎ Ｉｎｃ． エンコードライブラリの合成方法及び組成物
WO2019060857A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES

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Publication number Publication date
TWI905196B (zh) 2025-11-21
CA3185063A1 (en) 2021-12-02
JP2024167312A (ja) 2024-12-03
JPWO2021241528A1 (https=) 2021-12-02
EP4159766A1 (en) 2023-04-05
US20230212214A1 (en) 2023-07-06
WO2021241528A1 (ja) 2021-12-02
JP7838610B2 (ja) 2026-04-01
EP4159766A4 (en) 2024-06-19
KR20230015347A (ko) 2023-01-31
TW202212568A (zh) 2022-04-01
CN115461456A (zh) 2022-12-09

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