JP7511591B2 - 抗体の製造方法 - Google Patents

抗体の製造方法 Download PDF

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Publication number
JP7511591B2
JP7511591B2 JP2021577247A JP2021577247A JP7511591B2 JP 7511591 B2 JP7511591 B2 JP 7511591B2 JP 2021577247 A JP2021577247 A JP 2021577247A JP 2021577247 A JP2021577247 A JP 2021577247A JP 7511591 B2 JP7511591 B2 JP 7511591B2
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JP
Japan
Prior art keywords
codon
amino acid
nucleic acid
splice site
antibody
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JP2021577247A
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English (en)
Japanese (ja)
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JP2022538178A (ja
Inventor
ウルリッヒ ゴエプフェルト
ステファン クロスターマン
キャサリーナ ルッツ
ステファン セーベル
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of JP2022538178A publication Critical patent/JP2022538178A/ja
Priority to JP2023159322A priority Critical patent/JP7835713B2/ja
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2820/00—Vectors comprising a special origin of replication system
    • C12N2820/60—Vectors comprising a special origin of replication system from viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021577247A 2019-06-28 2020-06-25 抗体の製造方法 Active JP7511591B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023159322A JP7835713B2 (ja) 2019-06-28 2023-09-25 抗体の製造方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19183171 2019-06-28
EP19183171.8 2019-06-28
PCT/EP2020/067770 WO2020260431A1 (en) 2019-06-28 2020-06-25 Method for the production of an antibody

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023159322A Division JP7835713B2 (ja) 2019-06-28 2023-09-25 抗体の製造方法

Publications (2)

Publication Number Publication Date
JP2022538178A JP2022538178A (ja) 2022-08-31
JP7511591B2 true JP7511591B2 (ja) 2024-07-05

Family

ID=67253661

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2021577247A Active JP7511591B2 (ja) 2019-06-28 2020-06-25 抗体の製造方法
JP2023159322A Active JP7835713B2 (ja) 2019-06-28 2023-09-25 抗体の製造方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023159322A Active JP7835713B2 (ja) 2019-06-28 2023-09-25 抗体の製造方法

Country Status (11)

Country Link
US (1) US20220220500A1 (https=)
EP (1) EP3990484A1 (https=)
JP (2) JP7511591B2 (https=)
KR (1) KR102900433B1 (https=)
CN (1) CN113993888B (https=)
AU (1) AU2020304841B2 (https=)
BR (1) BR112021025806A2 (https=)
CA (1) CA3143803A1 (https=)
IL (1) IL289210A (https=)
MX (1) MX2021015193A (https=)
WO (1) WO2020260431A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010531648A (ja) 2007-06-29 2010-09-30 エフ．ホフマン−ラ ロシュ アーゲー 改善された免疫グロブリンの産生をもたらす重鎖変異体
JP2017508459A (ja) 2014-02-28 2017-03-30 グレンマーク ファーマシューティカルズ， エセ．アー． ポリペプチドを発現する宿主細胞を選択するための発現構築物および方法

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US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
WO1988007089A1 (en) 1987-03-18 1988-09-22 Medical Research Council Altered antibodies
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
AU4005289A (en) 1988-08-25 1990-03-01 Smithkline Beecham Corporation Recombinant saccharomyces
US5082767A (en) 1989-02-27 1992-01-21 Hatfield G Wesley Codon pair utilization
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
CN100427603C (zh) 1999-10-04 2008-10-22 麦迪卡格公司 调控外源基因转录的方法
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
JP2006238894A (ja) * 2000-02-15 2006-09-14 Amgen Inc 線維芽細胞成長因子−２３分子およびその使用
ATE318908T1 (de) 2000-05-18 2006-03-15 Geneart Gmbh Synthetische gene für gagpol und deren verwendungen
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
AU2003206684A1 (en) 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
US20040005600A1 (en) 2002-04-01 2004-01-08 Evelina Angov Method of designing synthetic nucleic acid sequences for optimal protein expression in a host cell
US20060024670A1 (en) 2004-05-18 2006-02-02 Luke Catherine J Influenza virus vaccine composition and methods of use
EA200700751A1 (ru) * 2004-10-05 2008-06-30 Элан Фарма Интернэшнл Лимитед Способы и композиции для улучшения продуцирования рекомбинантного белка
EP2021489A2 (en) 2006-05-30 2009-02-11 Dow Global Technologies Inc. Codon optimization method
DK2423315T3 (en) 2006-06-29 2015-04-13 Dsm Ip Assets Bv A method for obtaining enhanced polypeptide expression
US7947495B2 (en) * 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
AU2009314111A1 (en) * 2008-11-12 2010-05-20 Merck Sharp & Dohme Corp. BetaGI-IgG intron for enhanced anti-IGF1 R expression
US20100303776A1 (en) * 2009-04-16 2010-12-02 The University Of North Carolina At Chapel Hill Methods and compositions for regulated expression of multiple nucleic acids
KR20130013435A (ko) 2011-07-28 2013-02-06 차의과학대학교 산학협력단 태반-유래 줄기세포의 증식방법
CN109810188A (zh) * 2011-12-21 2019-05-28 弗·哈夫曼-拉罗切有限公司 用于克隆和表达关联抗体可变区基因区段的快速方法
EP2794878B1 (en) * 2011-12-22 2020-03-18 F.Hoffmann-La Roche Ag Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides
GB2502127A (en) * 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
JP6224077B2 (ja) * 2012-04-17 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改変された核酸を用いてポリペプチドを発現させるための方法
KR20180018659A (ko) * 2015-06-08 2018-02-21 코닝 인코포레이티드 전달이 없는 마이크로엘이디 디스플레이

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010531648A (ja) 2007-06-29 2010-09-30 エフ．ホフマン−ラ ロシュ アーゲー 改善された免疫グロブリンの産生をもたらす重鎖変異体
JP2017508459A (ja) 2014-02-28 2017-03-30 グレンマーク ファーマシューティカルズ， エセ．アー． ポリペプチドを発現する宿主細胞を選択するための発現構築物および方法

Also Published As

Publication number Publication date
EP3990484A1 (en) 2022-05-04
IL289210A (en) 2022-02-01
BR112021025806A2 (pt) 2022-02-08
KR102900433B1 (ko) 2025-12-15
AU2020304841B2 (en) 2024-08-15
US20220220500A1 (en) 2022-07-14
JP2023183417A (ja) 2023-12-27
CN113993888A (zh) 2022-01-28
MX2021015193A (es) 2022-01-18
JP2022538178A (ja) 2022-08-31
CA3143803A1 (en) 2020-12-30
KR20220024954A (ko) 2022-03-03
AU2020304841A1 (en) 2021-12-23
WO2020260431A1 (en) 2020-12-30
CN113993888B (zh) 2026-01-06
JP7835713B2 (ja) 2026-03-25

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