JP7497898B2 - High-density live fish transport box and transport method - Google Patents

Description

The present invention relates to the field of live fish transportation, and in particular to a live fish transport box and transport method that slows the deterioration of transport water quality and is suitable for long-distance transport of high-density fish.

Currently, the general practice for long-distance transport of fish is to place the fish in a PE bag with a certain volume of water, add oxygen to the water, tie up the PE bag, and finally place the fish in a polystyrene box or cooler box filled with ice for transport.

However, this form of transportation has the following shortcomings. First, the ice blocks come into direct contact with the fish, which easily injures the fish and makes the ice blocks melt, resulting in a short cooling time and an increase in the mortality rate of the fish as the temperature rises. Second, as the fish consume more and more oxygen during live transport, the oxygen-filled bag cannot maintain the level of dissolved oxygen in the water for a long time, and the fish easily die from lack of oxygen. Third, the oxygen-filled bag cannot perform water circulation and filtration, which accelerates the deterioration of water quality and makes the fish more susceptible to death from poisoning as toxic and harmful substances in the water accumulate (e.g., increased levels of substances such as ammonia nitrogen and nitrite, acidic water quality due to a decrease in pH value, etc.).

The object of the present invention is to provide a high-density live fish transport box and transport method that can maintain a low-temperature transport environment and dissolved oxygen level for a long period of time, effectively purify water quality, and is suitable for long-distance transport of live fish.

In order to achieve the above-mentioned object, one embodiment of the present invention relates to a high-density live fish transport box including a transport case and a transport cover, the transport case is divided into a fish transport area and a transport water circulation area, the transport cover in the live fish transport area is connected to the transport case by a rotating shaft, the transport cover is provided with an exhaust hole to prevent water splashing, a sealant is provided at the connection point between the transport cover and the transport case, the transport water circulation area is provided on the right side within the transport case, and specifically includes a water purification system, a refrigerator and an aeration system, the water purification system consists of four areas: primary filtration, secondary filtration, activated carbon filtration and ultraviolet sterilization, an openwork partition plate is provided between the primary filtration area and the fish transport area, an irregular porous filtration sieve and a porous sponge are provided in the primary filtration area, and an openwork partition plate is provided between the primary filtration area and the secondary filtration area. A high-density live fish transport box is provided in which a carved partition plate is provided, a filtration sponge is provided in the secondary filtration area, a fretwork partition plate is provided between the secondary filtration area and the activated carbon filtration area, activated carbon particles are provided in the activated carbon filtration area, a fretwork partition plate is provided between the activated carbon filtration area and the ultraviolet sterilization area, an ultraviolet lamp is provided in the ultraviolet sterilization area, a solid partition plate is provided between the ultraviolet sterilization area and the freezer, a drain hole is provided below the partition plate, a pump is provided in the ultraviolet sterilization area that is connected to the freezer intake port via the drain hole, the freezer drain port is connected to the case drain port, the aeration system is made up of an aeration device and an aerator, both are in close contact, the aeration device has a few aeration holes, the freezer and the aerator are in the dry area, and an exhaust port is provided between the dry area and the outside.

The above-mentioned technical proposals provided by the present invention can effectively slow the deterioration of water quality and improve the survival rate of live fish transported.

In one embodiment, the transport case and transport cover are made from PE material.

In one embodiment, an openwork partition is provided between the primary filtration area and the fish transport area.

In one embodiment, a porous filtration sieve and a porous sponge are provided in the primary filtration region.

In one embodiment, the porous filtration sieve is made from a PE material.

In one embodiment, an openwork partition plate is provided between the primary and secondary filtration areas.

In one embodiment, a filtration sponge is provided within the secondary filtration region.

In one embodiment, an openwork partition plate is provided between the secondary filtration area and the activated carbon filtration area.

In one embodiment, activated carbon particles are provided within the activated carbon filtration region.

In one embodiment, an openwork partition is provided between the activated carbon filtration area and the ultraviolet sterilization area.

In one embodiment, an ultraviolet lamp is provided within the ultraviolet sterilization area.

In one embodiment, the ultraviolet sterilization area is provided with a pump that is connected to the refrigerator intake through a drain.

In one embodiment, the refrigerator drain is connected to the case drain.

In one embodiment, the aeration device is attached to the aerator.

In one embodiment, an exhaust port is provided between the drying area where the aerator and the freezer are located and the outside.

The present invention further provides a method for transporting live fish using the transport box described above. The simple operation steps of the transport method are specifically as follows: (1) Temporary rearing: Temporary rearing is performed for 36 hours before transportation. During the temporary rearing period, the water temperature is 20-22°C, the salinity is 16‰, the dissolved oxygen is 4-6mg/L, the pH is 7.5-8.5, and the fish-water ratio is 1:50; (2) Pre-transportation treatment: Emulsified melissa oil is added to the transport water of the high-density live animal transport box (the method for emulsifying melissa oil is to mix uniformly for 3 minutes with V (essential oil):V (50% alcohol):V (Tween-80) = 10:200:1 to completely emulsify the essential oil), and the melissa oil is then added to the transport water of the high-density live animal transport box. The concentration of Lissa oil is 40mg/L, (3) cooling: fish are placed in a high-density live transport box with a fish-water ratio of 1:4, and then a refrigerator is set up to lower the water temperature from 20-22°C to the transport temperature of 12°C at a cooling rate of 3°C/h, (4) transport: the transport box is covered and sealed for transport, and during the transport period, the water temperature is 12(±1)°C for 72 hours, (5) recovery: after the transport is completed, the fish are placed in clean water that meets the fishery water quality standard with a water temperature of 12(±1)°C and allowed to recover at room temperature. The live transport method provided by the present invention can effectively delay the atrophy and deformation of gill filaments, reduce serum cortisol, stress proteins, blood glucose and caspase-3, expression levels, improve serum lysozyme and immunoglobulin levels, reduce oxidative stress reactions caused by transport, and improve the survival rate during transport.

1 is a three-view diagram of the overall structure of a high-density live animal transport box for fish in one embodiment of the present invention. FIG. FIG. 2 is a top view of the overall structure of the high-density living organism transport box for fish in the embodiment shown in FIG. 1. FIG. 2 is a perspective view of the overall structure of the high-density living organism transport box for fish in the embodiment shown in FIG. 1.

Below, an embodiment of the present invention will be described in detail with reference to the attached drawings, using specific examples as examples.

Figures 1 to 3 show one embodiment of the high-density live fish transport box of the present invention.

This high-density live transport box for fish 1 is divided into a fish transport area 2 and a transport water circulation area 3. A cover 4 is provided on the transport box 1 to prevent water from splashing during transport, and a rotating shaft connects the transport cover 4 above the transport water circulation area 3 to the transport box 1, and a sealant is provided at the connection point between the cover 4 and the transport box 1 to prevent water leakage, and the box and cover are made of environmentally friendly PE material. When aerated and transported, the gas in the box expands, so an exhaust hole 5 is provided on the cover 4 of the fish transport area 2 to prevent water splashing. The transport water circulation area 3 is provided on the right side in the transport box 1, and is divided into a water purification system 6, a refrigerator 7, and an aeration system 8 to maintain the level of dissolved oxygen in the water, a low-temperature transport environment, and effectively purify the water quality. The water purification system 6 consists of a primary filtration area 9, a secondary filtration area 10, an activated carbon filtration area 11, and an ultraviolet sterilization area 12. The water in the fish transport area 2 flows through the openwork partition plate 13, and large impurities are filtered out by the irregular porous filtering sieve and porous sponge provided in the primary filtering area 9. The primary filtered water flows from the primary filtering area 9 through the openwork partition plate 13, and small impurities are filtered out by the filtering sponge provided in the secondary filtering area 10. The secondary filtered water flows from the secondary filtering area 10 through the openwork partition plate 13, and harmful substances such as organic polymers and residual chlorine are absorbed by the activated carbon particles provided in the activated carbon filtering area 11. The water filtered by activated carbon flows from the activated carbon filtering area 11 through the openwork partition plate 13 into the ultraviolet sterilization area, where an ultraviolet lamp is provided to achieve the sterilization function, a solid partition plate 14 is provided between the ultraviolet sterilization area 12 and the refrigerator 7, and a drain hole 15 is provided below the partition plate. The pump provided in the ultraviolet sterilization area 12 is connected to the refrigerator 7 through the drain port below the partition plate, and the refrigerator 7 is connected to the case drain port 16, finally achieving a water circulation function that integrates freezing and filtering. The aeration system 8 is composed of an aeration device 17 and an aeration machine 18, where the aeration device 17 is in close contact with the aeration machine 18, and the aeration device 17 is provided with a few aeration holes 19 to achieve the aeration function in the transport box. Both the refrigerator 7 and the aeration machine 18 are in the dry area, and an exhaust port 20 is provided between this area and the outside. The above-mentioned technical proposals provided by the present invention can effectively delay the deterioration of water quality, reduce the stress response of fish during transportation, and improve the survival rate of live fish transported.

The following describes how to use the high-density live fish transport box provided by the present invention, using sea bass as an example.

In advance, the high-density livestock transport box was thoroughly washed and clean transport water was added, and emulsified Melissa Oil was added to the transport water at a concentration of 40 mg/L. The sea bass that had been temporarily reared for 36 h was placed in the high-density livestock transport box, and the water temperature was lowered from 20-22°C to the transport temperature of 12°C at a cooling rate of 3°C/h using a freezer. Finally, the transport box was covered and sealed for transport. During the transport period, the water temperature was 12 (±1)°C, salinity was 16‰, dissolved oxygen was 4-6 mg/L, pH was 7.5-8.5, and the fish water ratio was 1:4. After 72 h of transport, the sea bass arrived at the destination and was placed in clean water with a water temperature of 12 (±1)°C and allowed to recover at room temperature. The survival rate was 96%. After 12 h of recovery, the survival rate was 96%. When this technical solution was used to transport living organisms, 72 hours after the completion of the transport, the level of dissolved oxygen in the transport water and the survival rate of the transport were twice as high as those transported using normal oxygen-filled bags. The microstructure of the gill tissue was observed (observed using an optical microscope and a scanning electron microscope) and indicators such as oxidative stress (cortisol, stress proteins, blood sugar, caspase-3) and immune function (lysozyme, immunoglobulin) were measured. The experimental results showed that the application of Melissa oil can effectively delay the atrophy and deformation of the gill filaments, and has a significant effect on resistance to damage caused by oxidative stress and improving immunity.

From the above description of the specific embodiment of the present invention, it is found that the high-density live transport form for fish provided by the present invention has a significant beneficial effect of effectively alleviating stress reactions and tissue damage induced during transport, and improving the immune ability and survival rate of fish during transport, compared with the conventional technology. In particular, low-temperature transport under the conditions of water circulation filtration and aeration effectively ensures the maintenance of sufficient dissolved oxygen levels in the water, while low-temperature transport after adding Melissa oil effectively reduces the respiratory metabolic level of fish, improves the anti-stress ability and immune ability of fish, and achieves the effect of calming and relieving stress. The water purification system gradually filters and sterilizes the metabolic waste discharged by fish, effectively reduces the harmful bacteria and ammonia nitrogen levels in the transported water, and reliably guarantees healthy water quality during transport. The present invention creates favorable conditions and processes for live transport of fish, and improves the survival rate of live fish during transport.

The above-described embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Those skilled in the art may make modifications and changes to the above-described embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications and changes made by those skilled in the art without departing from the spirit and technical ideas disclosed in the present invention are also included in the scope of the claims of the present invention.

1 Transport box 2 Fish transport area 3 Transport water circulation area 4 Cover 5 Exhaust hole for preventing splashing 6 Water purification system 7 Refrigeration machine 8 Aeration system 9 Primary filtration area 10 Secondary filtration area 11 Activated carbon filtration area 12 Ultraviolet sterilization area 13 Openwork partition plate 14 Solid partition plate 15 Drainage hole 16 Case drain 17 Aeration device 18 Aerator 19 Aeration hole 20 Exhaust port

Claims (7)

A high-density live fish transport box including a transport case and a transport cover,
the transport cover covers an opening of the transport case;
The transport cover is provided with an exhaust hole;
A sealant is provided at a connection point between the transport cover and the case,
The transport case specifically includes a fish transport area and a transport water circulation area, and the transport water circulation area is provided on the right side of the transport case;
The transport water circulation area comprises a water purification system, a chiller and an aeration system;
The transport case and the transport cover are made of PE material;
A rotating shaft is connected between the transport cover and the transport case of the fish transport area;
A cover is provided in the transport water circulation area,
The water purification system comprises a primary filtration area, a secondary filtration area, an activated carbon filtration area, and an ultraviolet sterilization area;
The refrigerator is provided with a water inlet and a water outlet,
The aeration system includes an aeration device located in the fish transport area and an aerator located in the transport water circulation area, the aeration device is provided with a plurality of aeration holes, and the aeration device is connected to the aerator;
The fish transport area and the transport water circulation area are separated by a first openwork partition plate having an openwork portion, the openwork portion being located only in a portion of the first openwork partition plate between the primary filtration area and the fish transport area in the transport water circulation area, and a case drain is provided in a portion of the first openwork partition plate between the refrigerator and the fish transport area in the transport water circulation area;
The openwork portion of the first openwork partition plate is for allowing the water of the fish transport area to flow into the primary filtration area, a second openwork partition plate is provided between the primary filtration area and the secondary filtration area for allowing the water of the primary filtration area to flow into the secondary filtration area, a third openwork partition plate is provided between the secondary filtration area and the activated carbon filtration area for allowing the water of the secondary filtration area to flow into the activated carbon filtration area, and a third openwork partition plate is provided between the activated carbon filtration area and the ultraviolet sterilization area for allowing the water of the activated carbon filtration area to flow into the activated carbon filtration area. a fourth openwork partition plate is provided to allow water from the ultraviolet sterilization area to flow into the ultraviolet sterilization area, a solid partition plate is provided between the ultraviolet sterilization area and the freezer, a drain hole is provided below the solid partition plate, a pump provided in the ultraviolet sterilization area is connected to a water intake port provided in the freezer via the drain hole provided below the solid partition plate, and is for drawing water from the ultraviolet sterilization area into the freezer, and a drain port provided in the freezer is connected to the case drain port. An irregular porous filtering sieve and a porous sponge are provided within the primary filtering region;
The high-density live fish transport box according to claim 1, characterized in that the porous filtering sieve is made of PE material. The high-density fish transport box according to claim 1, characterized in that a filter sponge is provided in the secondary filtration area. The high-density fish transport box according to claim 1, characterized in that activated carbon particles are provided within the activated carbon filtering area. The high-density live fish transport box according to claim 1, characterized in that an ultraviolet sterilization lamp is provided in the ultraviolet sterilization area. The power supply condition of the refrigerator and the aerator in the transport case is AC voltage 220V.
2. The high-density live fish transport box according to claim 1, wherein an exhaust port is provided between the outside and a dry area in the transport case where the freezer and the aerator are located. (1) Temporary rearing: Temporary rearing for 36 hours before transportation. During the temporary rearing period, the water temperature was 20-22°C, the salinity was 16‰, the dissolved oxygen was 4-6mg/L, the pH was 7.5-8.5, and the fish-water ratio was 1:50.
(2) Pre-transport treatment: Melissa oil was emulsified by mixing 50% alcohol and Tween-80 in a volume ratio of 10:200:1 for 3 minutes, and the resulting mixture was added to the transport water of the high-density biological transport box at a concentration of 40 mg/L.
(3) Cooling: The fish are placed in a high-density live animal transport box with a fish-water ratio of 1:4, and then the water temperature is lowered from 20-22°C to the transport temperature of 12°C at a cooling rate of 3°C/h using a refrigerator;
(4) Transportation: The container is covered and sealed for transportation. During the transportation period, the water temperature is 12°C, and the temperature control accuracy is ±1°C. The container is transported for 72 hours.
(5) Recovery: After transportation, the fish are placed in clean water that meets the fishing water quality standards of 12°C water temperature and ±1°C temperature control accuracy, and the water temperature is allowed to recover to room temperature. This is a method for transporting fish using a transport box described in any one of claims 1 to 6 .