JP7475685B2 - ヒト真皮線維芽細胞の単離方法 - Google Patents

ヒト真皮線維芽細胞の単離方法 Download PDF

Info

Publication number
JP7475685B2
JP7475685B2 JP2020534824A JP2020534824A JP7475685B2 JP 7475685 B2 JP7475685 B2 JP 7475685B2 JP 2020534824 A JP2020534824 A JP 2020534824A JP 2020534824 A JP2020534824 A JP 2020534824A JP 7475685 B2 JP7475685 B2 JP 7475685B2
Authority
JP
Japan
Prior art keywords
human
dermal fibroblasts
fibroblasts
human dermal
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020534824A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021507714A (ja
Inventor
ワット フィオナ
フィリッペオス クリスティナ
リンチ マグナス
Original Assignee
キングス・カレッジ・ロンドン
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by キングス・カレッジ・ロンドン filed Critical キングス・カレッジ・ロンドン
Publication of JP2021507714A publication Critical patent/JP2021507714A/ja
Priority to JP2023218733A priority Critical patent/JP2024038101A/ja
Application granted granted Critical
Publication of JP7475685B2 publication Critical patent/JP7475685B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6881—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Rheumatology (AREA)
  • Dermatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020534824A 2017-12-22 2018-12-21 ヒト真皮線維芽細胞の単離方法 Active JP7475685B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023218733A JP2024038101A (ja) 2017-12-22 2023-12-26 ヒト真皮線維芽細胞の単離方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1721738.1 2017-12-22
GBGB1721738.1A GB201721738D0 (en) 2017-12-22 2017-12-22 Method
PCT/GB2018/053760 WO2019122931A1 (en) 2017-12-22 2018-12-21 Method for isolating human dermal fibroblasts

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023218733A Division JP2024038101A (ja) 2017-12-22 2023-12-26 ヒト真皮線維芽細胞の単離方法

Publications (2)

Publication Number Publication Date
JP2021507714A JP2021507714A (ja) 2021-02-25
JP7475685B2 true JP7475685B2 (ja) 2024-04-30

Family

ID=61131714

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2020534824A Active JP7475685B2 (ja) 2017-12-22 2018-12-21 ヒト真皮線維芽細胞の単離方法
JP2023218733A Pending JP2024038101A (ja) 2017-12-22 2023-12-26 ヒト真皮線維芽細胞の単離方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023218733A Pending JP2024038101A (ja) 2017-12-22 2023-12-26 ヒト真皮線維芽細胞の単離方法

Country Status (6)

Country Link
US (2) US20210095252A1 (enExample)
EP (1) EP3728565A1 (enExample)
JP (2) JP7475685B2 (enExample)
KR (1) KR102972156B1 (enExample)
GB (1) GB201721738D0 (enExample)
WO (1) WO2019122931A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202104130D0 (en) * 2021-03-24 2021-05-05 King S College London Method
WO2023090901A1 (ko) * 2021-11-18 2023-05-25 의료법인 성광의료재단 기계 학습을 이용한 세포 노화의 마커를 선별하는 방법, 세포 노화 바이오 마커, 및 이를 이용한 세놀리틱 제제를 스크리닝 하는 방법
CN115058388A (zh) * 2022-03-14 2022-09-16 福建医科大学附属协和医院 间充质干细胞创面修复优势功能亚群及其鉴定和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001215225A (ja) 1999-12-03 2001-08-10 L'oreal Sa 乳頭線維芽細胞に特異的な抗体の皮膚の質のマーカーとしての使用
JP2008271971A (ja) 2003-10-07 2008-11-13 Biomaster Inc 脂肪由来前駆細胞の細胞分化
JP2014533959A (ja) 2011-11-25 2014-12-18 シャネル パフュームズ ビューテ 乳頭状および網状の線維芽細胞の新規マーカーおよびその使用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070065415A1 (en) * 2005-09-16 2007-03-22 Kleinsek Donald A Compositions and methods for the augmentation and repair of defects in tissue

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001215225A (ja) 1999-12-03 2001-08-10 L'oreal Sa 乳頭線維芽細胞に特異的な抗体の皮膚の質のマーカーとしての使用
JP2008271971A (ja) 2003-10-07 2008-11-13 Biomaster Inc 脂肪由来前駆細胞の細胞分化
JP2014533959A (ja) 2011-11-25 2014-12-18 シャネル パフュームズ ビューテ 乳頭状および網状の線維芽細胞の新規マーカーおよびその使用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J Invest Dermatol, 2018 Mar 3, vol. 138, no. 4, pp. 811-825
Nature, 2013 Dec 12, vol. 504, no. 7479, pp. 277-281
Science, 2015 Apr 17, vol. 348, no. 6232, article no. aaa2151(RESEARCH ARTICLE SUMMARY p. 1, RESEARCH ARTICLE pp. 1-13)
TISSUE REGENERATION AND WOUND HEALING, 2017 May 01, vol. 137, no 5, Suppl. 1, p. S157 (abstract no. 914)

Also Published As

Publication number Publication date
US20240279609A1 (en) 2024-08-22
WO2019122931A1 (en) 2019-06-27
KR102972156B1 (ko) 2026-06-01
JP2024038101A (ja) 2024-03-19
KR20200103718A (ko) 2020-09-02
EP3728565A1 (en) 2020-10-28
US20210095252A1 (en) 2021-04-01
GB201721738D0 (en) 2018-02-07
JP2021507714A (ja) 2021-02-25

Similar Documents

Publication Publication Date Title
Philippeos et al. Spatial and single-cell transcriptional profiling identifies functionally distinct human dermal fibroblast subpopulations
Zhong et al. Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
US20240279609A1 (en) Method for isolating human dermal fibroblasts
Crigler et al. Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages
Middleton et al. Newt cells secrete extracellular vesicles with therapeutic bioactivity in mammalian cardiomyocytes
DK3183337T3 (en) Use of Jagged 1 / Frizzled 4 as cell surface marker for isolation of human cardiac ventricular progenitor cells
Maurizi et al. Human white adipocytes convert into “rainbow” adipocytes in vitro
TWI849298B (zh) 促進毛髮再生的富粒線體血漿、其製造方法及其用途
AU2008292042A1 (en) Population of adult stem cells derived from cardiac adipose tissue and use thereof in cardiac regeneration
Iwamiya et al. Cardiac fibroblast-derived VCAM-1 enhances cardiomyocyte proliferation for fabrication of bioengineered cardiac tissue
Resca et al. Enrichment in c-Kit improved differentiation potential of amniotic membrane progenitor/stem cells
US20250041346A1 (en) Thymic epithelial stem cells
Vidács et al. Phenotypic plasticity of melanocytes derived from human adult skin
JP2017538411A (ja) 組織適用におけるｌｇｒ４、ｌｇｒ５およびｌｇｒ６発現上皮幹細胞を用いた最小限分極機能性細胞微細凝集塊単位の開発および使用のための方法
Roy et al. Implanting mechanically reprogrammed fibroblasts for aged tissue regeneration and wound healing
Yildiz et al. Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs
US8334136B2 (en) Method for promoting hair growth or hair regeneration by maintaining or increasing expression of cell-adhesion factor
Sundaram et al. Differential immunomodulation of human mesenchymal stromal cells from various sources in an inflammation mimetic milieu
Gonzalez et al. Aging Adipose‐Derived Mesenchymal Stem Cells, Cultured on a Native Young Extracellular Matrix, Are Protected From Senescence and Apoptosis Along With Increased Expression of HLA‐DR and CD74 Associated With PI3K Signaling
HK1254739A1 (zh) 从年长细胞群中富集和扩增高潜能人间充质干细胞
Westcott et al. Single-nuclear transcriptomics of lymphedema-associated adipose reveals a pro-lymphangiogenic stromal cell population
Temme et al. Genetic profiling and surface proteome analysis of human atrial stromal cells and rat ventricular epicardium-derived cells reveals novel insights into their cardiogenic potential
WO2022200528A1 (en) Isolated primary dermal fibroblasts for treating skin conditions
Lombardi et al. Christina Philippeos1, Stephanie Telerman1, Bénédicte Oulès1, Angela O. Pisco1, Tanya J. Shaw2, Raul Elgueta3
Mazine et al. Generation of Valvular Interstitial Cells from Human Pluripotent Stem Cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201002

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211220

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20211220

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20221125

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221206

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20230227

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230601

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20230905

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231226

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20240119

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20240319

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20240410

R150 Certificate of patent or registration of utility model

Ref document number: 7475685

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150