JP7425592B2 - Powdered green tea extract composition - Google Patents
Powdered green tea extract composition Download PDFInfo
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- JP7425592B2 JP7425592B2 JP2019225511A JP2019225511A JP7425592B2 JP 7425592 B2 JP7425592 B2 JP 7425592B2 JP 2019225511 A JP2019225511 A JP 2019225511A JP 2019225511 A JP2019225511 A JP 2019225511A JP 7425592 B2 JP7425592 B2 JP 7425592B2
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- 235000020688 green tea extract Nutrition 0.000 title claims description 98
- 229940094952 green tea extract Drugs 0.000 title claims description 96
- 239000000203 mixture Substances 0.000 title claims description 60
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 claims description 51
- 235000005487 catechin Nutrition 0.000 claims description 51
- 150000001765 catechin Chemical class 0.000 claims description 48
- 229920000642 polymer Polymers 0.000 claims description 48
- 229910021645 metal ion Inorganic materials 0.000 claims description 44
- 239000003125 aqueous solvent Substances 0.000 claims description 25
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
- 239000007787 solid Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 6
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 4
- 229910001424 calcium ion Inorganic materials 0.000 claims description 4
- 229910001414 potassium ion Inorganic materials 0.000 claims description 4
- 229910001415 sodium ion Inorganic materials 0.000 claims description 4
- 239000004615 ingredient Substances 0.000 claims 1
- 238000000034 method Methods 0.000 description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
- 244000269722 Thea sinensis Species 0.000 description 19
- 238000003860 storage Methods 0.000 description 19
- 238000000605 extraction Methods 0.000 description 16
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- 238000005469 granulation Methods 0.000 description 11
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- 235000013616 tea Nutrition 0.000 description 11
- 239000003960 organic solvent Substances 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
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- 150000003839 salts Chemical class 0.000 description 8
- 238000005259 measurement Methods 0.000 description 7
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- 239000004927 clay Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
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- 238000002036 drum drying Methods 0.000 description 2
- XMOCLSLCDHWDHP-IUODEOHRSA-N epi-Gallocatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-IUODEOHRSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- LVJJFMLUMNSUFN-UHFFFAOYSA-N gallocatechin gallate Natural products C1=C(O)C=C2OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C1OC(=O)C1=CC(O)=C(O)C(O)=C1 LVJJFMLUMNSUFN-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
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- 230000001766 physiological effect Effects 0.000 description 2
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- 229910052700 potassium Inorganic materials 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
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- XMOCLSLCDHWDHP-SWLSCSKDSA-N (+)-Epigallocatechin Natural products C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-SWLSCSKDSA-N 0.000 description 1
- LSHVYAFMTMFKBA-PZJWPPBQSA-N (+)-catechin-3-O-gallate Chemical compound O([C@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=CC=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-PZJWPPBQSA-N 0.000 description 1
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- WMBWREPUVVBILR-GHTZIAJQSA-N (+)-gallocatechin gallate Chemical compound O([C@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-GHTZIAJQSA-N 0.000 description 1
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- LSHVYAFMTMFKBA-TZIWHRDSSA-N (-)-epicatechin-3-O-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=CC=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-TZIWHRDSSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000209507 Camellia Species 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- LSHVYAFMTMFKBA-UHFFFAOYSA-N ECG Natural products C=1C=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 LSHVYAFMTMFKBA-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- XMOCLSLCDHWDHP-UHFFFAOYSA-N L-Epigallocatechin Natural products OC1CC2=C(O)C=C(O)C=C2OC1C1=CC(O)=C(O)C(O)=C1 XMOCLSLCDHWDHP-UHFFFAOYSA-N 0.000 description 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930184207 Polyphenon Natural products 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
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- 230000002378 acidificating effect Effects 0.000 description 1
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- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
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- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
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- 150000003841 chloride salts Chemical class 0.000 description 1
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- 239000012153 distilled water Substances 0.000 description 1
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- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- DZYNKLUGCOSVKS-UHFFFAOYSA-N epigallocatechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3cc(O)c(O)c(O)c3 DZYNKLUGCOSVKS-UHFFFAOYSA-N 0.000 description 1
- 229940030275 epigallocatechin gallate Drugs 0.000 description 1
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- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 1
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- 150000002576 ketones Chemical class 0.000 description 1
- TYQCGQRIZGCHNB-JLAZNSOCSA-N l-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(O)=C(O)C1=O TYQCGQRIZGCHNB-JLAZNSOCSA-N 0.000 description 1
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- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
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Description
本発明は、粉末緑茶抽出組成物に関する。 The present invention relates to a powdered green tea extract composition.
近年、健康志向の高まりから、非重合体カテキン類の生理効果が注目されており、茶飲料等の飲食品の需要が拡大している。非重合体カテキン類による生理効果を十分に発現するには、高濃度の非重合体カテキン類を含む飲食品を継続して摂取することが有利である。そこで、緑茶抽出物を精製して夾雑物を除去し、非重合体カテキン類を高純度化する技術が種々検討されている。例えば、所定濃度の非重合体カテキン類を含有する固体カテキン製剤を、有機溶媒と水の重量比率が一定に制御された溶媒に添加し、これに水を加えることにより有機溶媒と水の重量比率を所定濃度に調整した後、不溶の固形分を除去する方法（特許文献１）、カテキン類組成物を、有機溶媒／水の質量比が一定に制御された混合溶媒に溶解させ、活性炭及び酸性白土又は活性白土と接触させる方法（特許文献２）、緑茶抽出物を、合成吸着剤を充填したカラムに通液して吸着させ、次いで水を通液してカラムを洗浄し、次いで所定濃度のエタノール水溶液をカラムに通液する方法（特許文献３）等が提案されている。 In recent years, as health consciousness has increased, the physiological effects of non-polymer catechins have been attracting attention, and the demand for food and drink products such as tea drinks is expanding. In order to fully express the physiological effects of non-polymer catechins, it is advantageous to continuously consume foods and drinks containing high concentrations of non-polymer catechins. Therefore, various techniques are being investigated to purify the green tea extract to remove impurities and to highly purify non-polymer catechins. For example, a solid catechin preparation containing non-polymer catechins at a predetermined concentration is added to a solvent in which the weight ratio of organic solvent and water is controlled to be constant, and by adding water to this, the weight ratio of organic solvent and water is controlled. After adjusting the concentration to a predetermined concentration, the insoluble solid content is removed (Patent Document 1), a catechin composition is dissolved in a mixed solvent with a constant mass ratio of organic solvent/water, and activated carbon and acidic A method of contacting with white clay or activated clay (Patent Document 2), green tea extract is passed through a column filled with a synthetic adsorbent to be adsorbed, water is then passed through the column to wash the column, and then a predetermined concentration of the green tea extract is adsorbed. A method of passing an ethanol aqueous solution through a column (Patent Document 3) and the like have been proposed.
一方、粉末飲料の粉体色及び飲用時の水色を改善する技術として、緑茶抽出物、抗酸化物、粉末茶を含有するインスタント緑茶にカリウムを一定量含有させることで、粉体色及び飲用時の水色を鮮やかな緑色に改善できることが報告されている（特許文献４）。しかし、かかる文献に記載された技術は、非重合体カテキン類を高純度化した緑茶抽出物を対象とするものでも、かかる緑茶抽出物自体の外観に着目したものでもない。 On the other hand, as a technology to improve the powder color of powdered drinks and the water color when drinking, by adding a certain amount of potassium to instant green tea containing green tea extract, antioxidants, and powdered tea, the powder color and water color when drinking are improved. It has been reported that the light blue of can be improved to bright green (Patent Document 4). However, the techniques described in such documents do not target green tea extracts in which non-polymer catechins are highly purified, nor do they focus on the appearance of such green tea extracts themselves.
通常、緑茶抽出組成物は、未精製の場合や非重合体カテキン類の純度が比較的低い場合には、粉末化しかつ遮光された条件であれば、長期に亘って保存したとしても、その外観に大きな変化は見られない。ところが、緑茶抽出組成物の精製度を増し非重合体カテキン類の純度を高めるにつれ、粉末化しかつ遮光したとしても、長期に亘って保存すると赤みを帯び、外観が大きく変化しやすいという課題があることを本発明者らは見出した。
したがって、本発明の課題は、高純度の非重合体カテキン類を含み、長期保存したときに色相変化のし難い粉末緑茶抽出組成物及びその製造方法を提供することにある。
Normally, if a green tea extract composition is unpurified or the purity of non-polymer catechins is relatively low, its appearance will change even if it is stored for a long time as long as it is powdered and protected from light. No major changes are seen. However, as the degree of purification of green tea extract compositions increases and the purity of non-polymer catechins increases, there is a problem that even if it is powdered and protected from light, it becomes reddish and easily changes its appearance when stored for a long period of time. The present inventors found that.
Therefore, an object of the present invention is to provide a powdered green tea extract composition that contains highly pure non-polymer catechins and is resistant to color change during long-term storage, and a method for producing the same.
本発明者らは、上記課題に鑑み検討した結果、（Ａ）非重合体カテキン類の含有量を特定量以上とした際に、該（Ａ）非重合体カテキン類に対する（Ｂ）金属イオンの質量比[（Ｂ）／（Ａ）]を特定値以上に制御することで、非重合体カテキン類を高純度で含有しながらも、長期保存したときの色相変化が抑制された粉末緑茶抽出組成物が得られることを見出した。 As a result of studies in view of the above-mentioned problems, the present inventors found that when the content of (A) non-polymer catechins is set to a specified amount or more, (B) metal ions are By controlling the mass ratio [(B)/(A)] to a specific value or more, a powdered green tea extract composition that suppresses hue change during long-term storage while containing high purity non-polymer catechins I found out that things can be obtained.
すなわち、次の成分（Ａ）及び（Ｂ）：
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０質量％以上であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上である、
粉末緑茶抽出組成物を提供するものである。
That is, the following components (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
The content of component (A) is 40% by mass or more, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.005 or more.
A powdered green tea extract composition is provided.
本発明はまた、（Ａ）非重合体カテキン類の含有量が４０質量％以上である緑茶抽出物と、（Ｂ）金属イオンとを、成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上となるように水系溶媒の存在下に混合した後、当該混合液を乾燥する工程を含む、粉末緑茶抽出組成物の製造方法を提供するものである。 The present invention also provides (A) a green tea extract having a content of non-polymer catechins of 40% by mass or more, and (B) metal ions in a mass ratio of component (B) to component (A) [( B)/(A)] is 0.005 or more in the presence of an aqueous solvent, and then drying the mixed liquid. .
本発明によれば、非重合体カテキン類を高純度で含有しながらも、長期保存したときに色相変化のし難い粉末緑茶抽出組成物を提供することができる。 According to the present invention, it is possible to provide a powdered green tea extract composition that contains non-polymer catechins with high purity and is difficult to change in color when stored for a long period of time.
〔粉末緑茶抽出組成物〕
本発明の粉末緑茶抽出組成物は、成分（Ａ）として非重合体カテキン類を含有する。ここで、本明細書において「非重合体カテキン類」とは、カテキン、ガロカテキン、エピカテキン及びエピガロカテキン等の非ガレート体と、カテキンガレート、ガロカテキンガレート、エピカテキンガレート及びエピガロカテキンガレート等のガレート体を併せての総称である。本発明においては、上記８種の非重合体カテキン類のうち少なくとも１種を含有すればよい。
[Powdered green tea extract composition]
The powdered green tea extract composition of the present invention contains non-polymer catechins as component (A). Here, in this specification, "non-polymer catechins" include non-gallates such as catechin, gallocatechin, epicatechin, and epigallocatechin, and catechin gallate, gallocatechin gallate, epicatechin gallate, epigallocatechin gallate, etc. It is a general term for all gallate bodies. In the present invention, it is sufficient to contain at least one of the eight types of non-polymer catechins mentioned above.
本発明の粉末緑茶抽出組成物は、成分（Ａ）の含有量が４０質量％以上であるが、非重合体カテキン類の強化の観点から、４２質量％以上が好ましく、４４質量％以上がより好ましく、４６質量％以上が更に好ましく、また長期保存したときの色相変化の抑制、生産効率の観点から、９５質量％以下が好ましく、９０質量％以下がより好ましく、８０質量％以下が更に好ましく、７０質量％以下がより更に好ましく、６０質量％以下がより更に好ましい。かかる成分（Ａ）の含有量の範囲としては、本発明の粉末緑茶抽出組成物中に、好ましくは４０～９５質量％であり、より好ましくは４２～９０質量％であり、更に好ましくは４４～８０質量％であり、更に好ましくは４６～７０質量％でありより更に好ましくは４６～６０質量％である。成分（Ａ）の含有量は、上記８種の合計量に基づいて定義され、通常知られている測定法のうち測定試料の状況に適した分析法により測定することができる。例えば、液体クロマトグラフィで分析することが可能であり、具体的には、後掲の実施例に記載の方法が挙げられる。また、測定の際には装置の検出域に適合させるため、試料を凍結乾燥したり、装置の分離能に適合させるため試料中の夾雑物を除去したりする等、必要に応じて適宜処理を施してもよい。 In the powdered green tea extract composition of the present invention, the content of component (A) is 40% by mass or more, but from the viewpoint of strengthening non-polymer catechins, the content is preferably 42% by mass or more, and more preferably 44% by mass or more. Preferably, 46% by mass or more, more preferably 46% by mass or more, and from the viewpoint of suppressing hue change during long-term storage and production efficiency, 95% by mass or less, more preferably 90% by mass or less, even more preferably 80% by mass or less, It is even more preferably 70% by mass or less, even more preferably 60% by mass or less. The content range of component (A) in the powdered green tea extract composition of the present invention is preferably 40 to 95% by mass, more preferably 42 to 90% by mass, and still more preferably 44 to 95% by mass. The amount is 80% by weight, more preferably 46-70% by weight, even more preferably 46-60% by weight. The content of component (A) is defined based on the total amount of the above eight types, and can be measured by an analytical method suitable for the situation of the measurement sample among commonly known measuring methods. For example, it is possible to analyze by liquid chromatography, and specific examples include the method described in Examples below. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.
本発明の粉末緑茶抽出組成物は、成分（Ｂ）として金属イオンを含有する。
成分（Ｂ）としては、食品として許容される金属イオンであれば特に限定されないが、例えば、１価の金属イオン、２価の金属イオン、３価以上の金属イオンを挙げることができる。１価の金属イオンとしては、例えば、ナトリウムイオン、カリウムイオンが挙げられる。また、２価の金属イオンとしては、例えば、カルシウムイオン、マグネシウムイオンを挙げることができる。３価以上の金属イオンとしては、例えば、アルミニウムイオンが挙げられる。成分（Ｂ）は、１種又は２種以上含有することができる。
The powdered green tea extract composition of the present invention contains metal ions as component (B).
Component (B) is not particularly limited as long as it is a metal ion that is acceptable as a food product, and examples thereof include monovalent metal ions, divalent metal ions, and trivalent or higher valent metal ions. Examples of monovalent metal ions include sodium ions and potassium ions. Furthermore, examples of divalent metal ions include calcium ions and magnesium ions. Examples of the trivalent or higher valence metal ions include aluminum ions. Component (B) may be contained alone or in combination of two or more.
中でも、長期保存したときの色相変化の抑制の観点から、１価の金属イオン及び２価の金属イオンから選ばれる１種又は２種以上含むことが好ましく、ナトリウムイオン、カリウムイオン及びカルシウムイオンから選ばれる１種又は２種以上含むことが更に好ましい。 Among these, from the viewpoint of suppressing hue change during long-term storage, it is preferable to contain one or more selected from monovalent metal ions and divalent metal ions, and preferably from sodium ions, potassium ions, and calcium ions. It is more preferable to include one or more types.
成分（Ｂ）は、原料に由来するものでも、新たに加えたものでもよい。
また、成分（Ｂ）は、粉末緑茶抽出組成物を水に溶解したときに金属イオンを生成するものであれば、塩の形態でもよい。塩の形態としては食品として許容されるものであれば特に限定されないが、例えば、無機塩、有機酸塩を挙げることができる。無機塩としては、例えば、塩化物塩、リン酸塩、炭酸塩を挙げることができる。有機酸塩としては、例えば、酢酸、アジピン酸、アスコルビン酸、酪酸、クエン酸、ギ酸、フマル酸、グリコン酸、乳酸、リンゴ酸、シュウ酸、コハク酸、酒石酸等の食用酸塩を挙げることができる。
Component (B) may be derived from raw materials or may be newly added.
In addition, component (B) may be in the form of a salt as long as it generates metal ions when the powdered green tea extract composition is dissolved in water. The form of the salt is not particularly limited as long as it is acceptable as a food, but examples include inorganic salts and organic acid salts. Examples of inorganic salts include chloride salts, phosphates, and carbonates. Examples of organic acid salts include edible acid salts such as acetic acid, adipic acid, ascorbic acid, butyric acid, citric acid, formic acid, fumaric acid, glyconic acid, lactic acid, malic acid, oxalic acid, succinic acid, and tartaric acid. can.
本発明の粉末緑茶抽出組成物は、成分（Ｂ）の含有量が、長期保存したときの色相変化の抑制の観点から、０．２質量％以上であることが好ましく、１．０質量％以上がより好ましく、２．０質量％以上が更に好ましく、３．０質量％以上がより更に好ましく、４．０質量％以上がより更に好ましく、また長期保存したときの色相変化の抑制、食品への配合のし易さの観点から、１４質量％以下が好ましく、１２質量％以下がより好ましく、１０質量％以下が更に好ましい。かかる成分（Ｂ）の含有量の範囲としては、本発明の粉末緑茶抽出組成物中に、好ましくは０．２～１４質量％であり、より好ましくは１．０～１２質量％であり、更に好ましくは２．０～１２質量％であり、より更に好ましくは３．０～１０質量％であり、より更に好ましくは４．０～１０質量％である。なお、成分（Ｂ）の含有量は、金属イオンの合計量とする。ここで、本明細書において「成分（Ｂ）の含有量」は、原子吸光分析により測定したものであって、具体的には、後掲の実施例に記載の方法により測定することができる。また、測定の際には装置の検出域に適合させるため、試料を凍結乾燥したり、装置の分離能に適合させるため試料中の夾雑物を除去したりする等、必要に応じて適宜処理を施してもよい。 In the powdered green tea extract composition of the present invention, the content of component (B) is preferably 0.2% by mass or more, and preferably 1.0% by mass or more from the viewpoint of suppressing hue change during long-term storage. is more preferable, 2.0% by mass or more is still more preferable, 3.0% by mass or more is even more preferable, 4.0% by weight or more is even more preferable, and it also suppresses hue change during long-term storage and prevents food from being affected. From the viewpoint of ease of blending, the content is preferably 14% by mass or less, more preferably 12% by mass or less, and even more preferably 10% by mass or less. The content range of component (B) in the powdered green tea extract composition of the present invention is preferably 0.2 to 14% by mass, more preferably 1.0 to 12% by mass, and Preferably it is 2.0 to 12% by weight, even more preferably 3.0 to 10% by weight, even more preferably 4.0 to 10% by weight. Note that the content of component (B) is the total amount of metal ions. Here, in this specification, the "content of component (B)" is measured by atomic absorption spectrometry, and specifically, it can be measured by the method described in Examples below. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.
本発明の粉末緑茶抽出組成物は、成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上であるが、長期保存したときの色相変化の抑制の観点から、０．０１０以上が好ましく、０．０２以上がより好ましく、０．０４以上が更に好ましく、０．０９以上がより更に好ましい。また、かかる質量比[（Ｂ）／（Ａ）]は、風味、本発明の効果を享受しやすい点から、０．３以下が好ましく、０．２５以下がより好ましく、０．２以下が更に好ましい。かかる質量比[（Ｂ）／（Ａ）]の範囲としては、好ましくは０．００５～０．３であり、より好ましくは０．０１０～０．３であり、更に好ましくは０．０２～０．３であり、より更に好ましくは０．０４～０．２５であり、殊更に好ましくは０．０９～０．２である。 The powdered green tea extract composition of the present invention has a mass ratio of component (B) to component (A) [(B)/(A)] of 0.005 or more, but is effective in suppressing hue change during long-term storage. From the viewpoint, it is preferably 0.010 or more, more preferably 0.02 or more, even more preferably 0.04 or more, and even more preferably 0.09 or more. In addition, from the viewpoint of flavor and ease of enjoying the effects of the present invention, the mass ratio [(B)/(A)] is preferably 0.3 or less, more preferably 0.25 or less, and even more preferably 0.2 or less. preferable. The range of the mass ratio [(B)/(A)] is preferably 0.005 to 0.3, more preferably 0.010 to 0.3, and even more preferably 0.02 to 0. .3, even more preferably from 0.04 to 0.25, even more preferably from 0.09 to 0.2.
また、本発明の粉末緑茶抽出組成物は、長期保存したときの色相変化の抑制、風味、ブロッキング防止の観点から、揮発分が１０．０質量％以下であることが好ましく、８．０質量％以下がより好ましく、５質量％以下が更に好ましい。また、生産効率の観点から、揮発分は、０．２質量％以上が好ましく、０．４質量％以上がより好ましく、０．６質量％以上が更に好ましい。かかる揮発分の範囲としては、好ましくは０．２～１０．０質量％であり、より好ましくは０．４～８．０質量％であり、更に好ましくは０．６～５．０質量％である。ここで、本明細書において「揮発分」とは、試料を１０５℃の電気恒温乾燥機で１５分間加熱したときの揮発成分の含有割合をいい、加熱前後の試料の質量から、下記式（１）により算出される値をいう。揮発分は、例えば、水と、水以外の揮発性物質とを包含する概念である。 In addition, the powdered green tea extract composition of the present invention preferably has a volatile content of 10.0% by mass or less, preferably 8.0% by mass, from the viewpoint of suppressing hue change, flavor, and preventing blocking during long-term storage. The following is more preferable, and 5% by mass or less is even more preferable. Moreover, from the viewpoint of production efficiency, the volatile content is preferably 0.2% by mass or more, more preferably 0.4% by mass or more, and even more preferably 0.6% by mass or more. The range of such volatile content is preferably 0.2 to 10.0% by mass, more preferably 0.4 to 8.0% by mass, and still more preferably 0.6 to 5.0% by mass. be. Here, in this specification, "volatile content" refers to the content ratio of volatile components when a sample is heated for 15 minutes in an electric constant temperature dryer at 105°C, and is calculated from the mass of the sample before and after heating by the following formula (1 ) refers to the value calculated by Volatile matter is a concept that includes, for example, water and volatile substances other than water.
揮発分(質量％)＝（Ｐ－Ｑ）／Ｐ×１００ （１） Volatile content (mass%) = (P-Q)/P x 100 (1)
〔式中、Ｐは、加熱前の試料の質量を示し、Ｑは、加熱後の試料の質量を示す。〕 [In the formula, P represents the mass of the sample before heating, and Q represents the mass of the sample after heating. ]
本明細書において「粉末」とは、粒子状形態を意味し、粉末状のみならず、顆粒状も包含する概念である。本発明の粉末緑茶抽出組成物の形態としては、取り扱いのし易さ、溶解性の観点から、粉末状が好ましい。 In this specification, "powder" means a particulate form, and is a concept that includes not only powder form but also granule form. The powdered green tea extract composition of the present invention is preferably in powder form from the viewpoint of ease of handling and solubility.
本発明の粉末緑茶抽出組成物は、平均粒子径が、溶解時の凝集（継粉）抑制の観点から、１μｍ以上であることが好ましく、１０μｍ以上がより好ましく、２０μｍ以上更に好ましく、また溶解性の観点から、１０００μｍ以下が好ましく、８００μｍ以下がより好ましく、５００μｍ以下が更に好ましい。かかる平均粒子径の範囲としては、好ましくは１～１０００μｍであり、より好ましくは１０～８００μｍであり、更に好ましくは２０～５００μｍである。ここで、本明細書において「平均粒子径」とは、レーザー回折・散乱法により測定したものであって、粉末緑茶抽出組成物の粒度分布を個数基準で作成して得られたメディアン径（ｄ50）を平均粒子径としたものである。 The powdered green tea extract composition of the present invention preferably has an average particle diameter of 1 μm or more, more preferably 10 μm or more, even more preferably 20 μm or more, from the viewpoint of suppressing aggregation (splicing powder) during dissolution, and From this viewpoint, the thickness is preferably 1000 μm or less, more preferably 800 μm or less, and even more preferably 500 μm or less. The average particle size range is preferably 1 to 1000 μm, more preferably 10 to 800 μm, and still more preferably 20 to 500 μm. Here, in this specification, the "average particle diameter" is measured by laser diffraction/scattering method, and is the median diameter (d 50 ) as the average particle diameter.
本発明の粉末緑茶抽出組成物は、長期保存したときの色相変化が抑制されている。具体的には、３７℃／５０％ＲＨの雰囲気下で８週間保存した後の粉末緑茶抽出組成物のａ*値と、保存開始前（例えば、製造直後）の粉末緑茶抽出組成物のａ1 *値との差分の絶対値Δａ*（｜ａ*－ａ1 *｜）を、通常６以下、好ましくは５以下、より好ましくは４以下、更に好ましくは３以下、より更に好ましくは２以下、殊更に好ましくは１．５以下とすることができる。 The powdered green tea extract composition of the present invention exhibits suppressed hue change during long-term storage. Specifically, the a * value of the powdered green tea extract composition after being stored for 8 weeks in an atmosphere of 37°C/50% RH, and the a 1 value of the powdered green tea extract composition before the start of storage (for example, immediately after production). * The absolute value of the difference Δa * (|a * - a 1 * |) is usually 6 or less, preferably 5 or less, more preferably 4 or less, even more preferably 3 or less, even more preferably 2 or less, It is particularly preferably 1.5 or less.
なお、３７℃／５０％ＲＨの雰囲気下で８週間保存という条件は、通常、２５℃±２℃／６０％ＲＨ±５％で約１０ヶ月保存したときに相当する。ここで、本明細書において「ａ*値」とは、色をＬ*ａ*ｂ*表色系で表現したときに、色相、彩度を表す座標値であって、赤色方向の彩度を示す座標値である。Ｌ*ａ*ｂ*表色系には、黄色方向の彩度を示す座標値であるｂ*もあるが、本発明においては、粉末緑茶抽出組成物が色相変化したときに最も顕在化しやすいａ*について規定するものである。なお、ａ*値の測定は、後掲の実施例に記載の「色相変化の測定」にしたがうものとする。 Note that the condition of storage for 8 weeks in an atmosphere of 37° C./50% RH usually corresponds to storage for about 10 months at 25° C.±2° C./60% RH±5%. Here, in this specification, "a * value" is a coordinate value representing hue and saturation when a color is expressed in the L * a * b * color system, and is a coordinate value that represents the saturation in the red direction. These are the coordinate values shown. Although the L * a * b * color system also includes b * , which is a coordinate value indicating the saturation in the yellow direction, in the present invention, a is the coordinate value that is most likely to become apparent when the powdered green tea extract composition changes in hue. * It stipulates. Note that the a * value is measured in accordance with "Measurement of Hue Change" described in Examples below.
〔粉末緑茶抽出組成物の製造方法〕
本発明の粉末緑茶抽出組成物は、上記特性を具備すれば適宜の方法で製造することが可能であり、特に限定されないが、例えば、（ａ）固形分中の（Ａ）非重合体カテキン類の含有量が４０質量％以上である緑茶抽出物と、（Ｂ）金属イオンとを、成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上となる割合で、水系溶媒の存在下に混合した後、混合液を乾燥する工程を含む方法により製造することができる。ここで、本明細書において「固形分」とは、試料を１０５℃の電気恒温乾燥機で３時間乾燥して揮発成分を除いた残分をいう。
[Method for producing powdered green tea extract composition]
The powdered green tea extract composition of the present invention can be produced by an appropriate method as long as it has the above characteristics, and is not particularly limited, but for example, (A) non-polymer catechins in (a) solid content. A green tea extract having a content of 40% by mass or more and (B) metal ions are combined so that the mass ratio of component (B) to component (A) [(B)/(A)] is 0.005 or more. It can be produced by a method that includes a step of mixing in the presence of an aqueous solvent at a ratio of 0.05 to 1.00 ml, and then drying the mixed solution. Here, in this specification, "solid content" refers to the residue obtained by drying a sample in an electric constant temperature dryer at 105° C. for 3 hours and removing volatile components.
（ａ）緑茶抽出物は、原料緑茶抽出物を精製して夾雑物を除去し、固形分中の（Ａ）非重合体カテキン類の含有量を４０質量％以上に調整したものである。
原料緑茶抽出物は、通常、緑茶葉から抽出して得られることができる。緑茶葉としては、例えば、Camellia属の茶葉、例えば、C.sinensis.var.sinensis（やぶきた種を含む）、C.sinensis.var.assamica及びそれらの雑種から選択される茶葉（Camellia sinensis）を挙げることができる。具体的には、例えば、煎茶、深蒸し煎茶、焙じ茶、番茶、玉露、かぶせ茶、碾茶、釜入り茶、茎茶、棒茶、芽茶等が挙げられる。なお、茶葉の茶品種及び採取時期は特に限定されず、適宜選択することができる。
(a) Green tea extract is obtained by refining the raw green tea extract to remove impurities and adjusting the content of (A) non-polymer catechins in the solid content to 40% by mass or more.
The raw green tea extract can usually be obtained by extracting from green tea leaves. As the green tea leaves, for example, tea leaves of the genus Camellia, for example, tea leaves selected from C.sinensis.var.sinensis (including Yabukita species), C.sinensis.var.assamica, and hybrids thereof (Camellia sinensis) are used. can be mentioned. Specifically, examples include sencha, deep-steamed sencha, roasted tea, bancha, gyokuro, kabusecha, tencha, potted tea, stem tea, stick tea, and bud tea. Note that the tea variety and harvesting time of the tea leaves are not particularly limited and can be selected as appropriate.
抽出方法としては、ニーダー抽出、攪拌抽出（バッチ抽出）、向流抽出（ドリップ抽出）、カラム抽出等の公知の方法を採用することができる。
抽出条件は、抽出方法により適宜選択することが可能であるが、例えば、抽出溶媒として、水系溶媒を使用することができる。ここで、本明細書において「水系溶媒」とは、水を含む溶媒をいい、水系溶媒中の水の含有量は通常１～１００質量％、好ましくは３０～１００質量％である。水系溶媒としては、例えば、水、有機溶媒水溶液、炭酸水等が挙げられる。有機溶媒としては、例えば、アルコール、ケトン、エステル等が挙げられ、食品への使用を考慮すると、アルコールが好ましく、エタノールが更に好ましい。中でも、水系溶媒としては、水が好ましい。水としては、例えば、水道水、天然水、蒸留水、イオン交換水等が挙げられる。中でも、味の面から、イオン交換水が好ましい。
As the extraction method, known methods such as kneader extraction, stirring extraction (batch extraction), countercurrent extraction (drip extraction), and column extraction can be employed.
Extraction conditions can be selected as appropriate depending on the extraction method; for example, an aqueous solvent can be used as the extraction solvent. Here, in this specification, the term "aqueous solvent" refers to a solvent containing water, and the content of water in the aqueous solvent is usually 1 to 100% by mass, preferably 30 to 100% by mass. Examples of the aqueous solvent include water, an aqueous organic solvent solution, carbonated water, and the like. Examples of the organic solvent include alcohol, ketone, ester, etc. Considering use in foods, alcohol is preferable, and ethanol is more preferable. Among these, water is preferred as the aqueous solvent. Examples of water include tap water, natural water, distilled water, ion exchange water, and the like. Among these, ion-exchanged water is preferred from the standpoint of taste.
水系溶媒のｐＨ（２０℃）は、通常４～１０、好ましくは５～７である。なお、所望のｐＨとするために、水系溶媒にｐＨ調整剤を添加してもよい。
水系溶媒の温度は、通常２５～１００℃、好ましくは５０～１００℃、更に好ましくは８０～９９℃である。
抽出倍率及び抽出時間は、抽出方法やスケールにより一様ではないが、例えば、抽出倍率は通常１～５０倍であり、抽出時間は通常５～６０分である。
抽出後、濾過、遠心分離、膜処理等の公知の固液分離手段により、緑茶葉と原料緑茶抽出物とを分離することができる。
The pH (20° C.) of the aqueous solvent is usually 4 to 10, preferably 5 to 7. Note that a pH adjuster may be added to the aqueous solvent in order to obtain a desired pH.
The temperature of the aqueous solvent is usually 25 to 100°C, preferably 50 to 100°C, and more preferably 80 to 99°C.
Although the extraction magnification and extraction time vary depending on the extraction method and scale, for example, the extraction magnification is usually 1 to 50 times, and the extraction time is usually 5 to 60 minutes.
After extraction, the green tea leaves and the raw green tea extract can be separated by known solid-liquid separation means such as filtration, centrifugation, and membrane treatment.
また、原料緑茶抽出物として市販品を使用してもよい。例えば、三井農林社製の「ポリフェノン」、伊藤園社製の「テアフラン」、太陽化学社製の「サンフェノン」等が挙げられる。 Furthermore, a commercially available product may be used as the raw material green tea extract. Examples include "Polyphenon" manufactured by Mitsui Norin Co., Ltd., "Theafran" manufactured by Itoen Co., Ltd., and "Sunfenon" manufactured by Taiyo Kagaku Co., Ltd.
精製方法としては、固形分中の（Ａ）非重合体カテキン類の含有量が４０質量％以上となれば特に限定されないが、風味の観点、本発明の効果を享受しやすい観点から、下記（ｉ）～（iii）のいずれか一の方法、あるいは二以上の組み合わせを挙げることができる。
（ｉ）原料緑茶抽出物を、水又は有機溶媒水溶液（例えば、エタノール水溶液）に懸濁し、生じた沈殿を除去する方法。
（ii）原料緑茶抽出物を、活性炭、酸性白土及び活性白土から選択される少なくとも１種の吸着剤と接触させる方法。
（iii）原料緑茶抽出物を、合成吸着剤又はイオン交換樹脂と接触させる方法。
The purification method is not particularly limited as long as the content of (A) non-polymer catechins in the solid content is 40% by mass or more, but from the viewpoint of flavor and ease of enjoying the effects of the present invention, the following ( Any one of the methods i) to (iii) or a combination of two or more can be used.
(i) A method in which a raw green tea extract is suspended in water or an aqueous organic solvent solution (for example, an aqueous ethanol solution) and the resulting precipitate is removed.
(ii) A method of bringing a raw green tea extract into contact with at least one adsorbent selected from activated carbon, acid clay, and activated clay.
(iii) A method of bringing the raw green tea extract into contact with a synthetic adsorbent or ion exchange resin.
上記（ｉ）の方法については、例えば、特開２００４－１４７５０８号公報、特開２００４－１４９４１６号公報等を参照することができる。また、上記（ii）の方法については、例えば、特開２００４－２２２７１９号公報、特開２００５－２７００９４号公報等を参照することができる。更に、上記（iii）の方法については、例えば、特開２００４－２２２５９２号公報、特開２００６－８５８０号公報、特開２００６－１６０６５６号公報等を参照することができる。
また、上記（ｉ）～（iii）の方法においては、原料緑茶抽出物としてタンナーゼ処理したものを使用してもよく、また（ｉ）～（iii）のいずれか一以上の方法により処理した後、タンナーゼ処理することもできる。ここで、「タンナーゼ処理」とは、緑茶抽出物を、タンナーゼ活性を有する酵素と接触させることをいう。なお、タンナーゼ処理における具体的な操作方法は公知の方法を採用することが可能であり、例えば、特開２００４－３２１１０５号公報に記載の方法を挙げることができる。
Regarding the method (i) above, reference can be made to, for example, Japanese Patent Application Publication No. 2004-147508, Japanese Patent Application Publication No. 2004-149416, and the like. Further, regarding the method (ii) above, reference can be made to, for example, Japanese Patent Laid-Open Nos. 2004-222719 and 2005-270094. Further, regarding the method (iii) above, reference may be made to, for example, Japanese Patent Application Publication No. 2004-222592, Japanese Patent Application Publication No. 2006-8580, and Japanese Patent Application Publication No. 2006-160656.
In addition, in the methods (i) to (iii) above, a tannase-treated green tea extract may be used as the raw material green tea extract, or after treatment by one or more of the methods (i) to (iii), , and can also be treated with tannase. Here, "tannase treatment" refers to bringing the green tea extract into contact with an enzyme having tannase activity. Note that a known method can be adopted as a specific operating method for the tannase treatment, and for example, the method described in JP-A No. 2004-321105 can be mentioned.
このようにして得られた（ａ）緑茶抽出物は、固形分中の（Ａ）非重合体カテキン類の含有量が４０質量％以上であるが、風味の観点、本発明の効果を享受しやすい観点から、固形分中の（Ａ）非重合体カテキン類の含有量が４５質量％以上であることが好ましく、５０質量％以上がより好ましく、５５質量％以上が更に好ましい。なお、固形分中の（Ａ）非重合体カテキン類の含有量の上限値は１００質量％であっても構わないが、生産効率の観点から、９５質量％以下が好ましく、９０質量％以下がより好ましく、８５質量％以下が更に好ましい。 The green tea extract (a) obtained in this manner has a content of (A) non-polymer catechins in the solid content of 40% by mass or more, but does not enjoy the effects of the present invention from the viewpoint of flavor. From the viewpoint of simplicity, the content of (A) non-polymer catechins in the solid content is preferably 45% by mass or more, more preferably 50% by mass or more, and even more preferably 55% by mass or more. The upper limit of the content of (A) non-polymer catechins in the solid content may be 100% by mass, but from the viewpoint of production efficiency, it is preferably 95% by mass or less, and 90% by mass or less. It is more preferably 85% by mass or less.
（ａ）緑茶抽出物は、そのままでも、濃縮又は乾燥して使用しても構わない。濃縮方法としては、例えば、常圧にて溶媒の蒸発を行う常圧濃縮法、減圧にて溶媒の蒸発を行う減圧濃縮法、膜分離により溶媒を除去する膜濃縮法等が挙げられる。また、乾燥方法としては、例えば、噴霧乾燥、ドラム乾燥、凍結乾燥等の公知の方法を採用することができる。 (a) The green tea extract may be used as it is or after being concentrated or dried. Examples of the concentration method include a normal pressure concentration method in which the solvent is evaporated at normal pressure, a vacuum concentration method in which the solvent is evaporated under reduced pressure, and a membrane concentration method in which the solvent is removed by membrane separation. Further, as the drying method, for example, known methods such as spray drying, drum drying, freeze drying, etc. can be employed.
次に、（ａ）緑茶抽出物と（Ｂ）金属イオンとを所定の割合で、水性溶媒の存在下に混合して混合液を調製する。なお、成分（Ｂ）は、塩の形態で含有させてもよく、例えば、無機塩、有機酸塩の形態で含有させることができる。塩の具体例は、上記において説明したとおりである。
（ａ）緑茶抽出物、（Ｂ）金属イオンの混合順序は特に限定されず、これらを任意の順序で添加しても、両者を同時に添加してもよく、乾燥前の混合液中に水系溶媒と、（ａ）緑茶抽出物と、（Ｂ）金属イオンとが共存した状態にあればよい。
Next, (a) green tea extract and (B) metal ions are mixed at a predetermined ratio in the presence of an aqueous solvent to prepare a mixed solution. Note that component (B) may be contained in the form of a salt, for example, in the form of an inorganic salt or an organic acid salt. Specific examples of the salt are as explained above.
The mixing order of (a) green tea extract and (B) metal ions is not particularly limited, and they may be added in any order or both may be added at the same time. , (a) green tea extract, and (B) metal ions may coexist.
（ａ）緑茶抽出物と（Ｂ）金属イオンは、（Ａ）非重合体カテキン類に対する（Ｂ）金属イオンの質量比[（Ｂ）／（Ａ）]が０．００５以上となる割合で混合するが、かかる質量比[（Ｂ）／（Ａ）]は、長期保存したときの色相変化の抑制の観点から、０．０１０以上が好ましく、０．０２以上がより好ましく、０．０４以上が更に好ましく、０．０９以上がより更に好ましい。また、かかる質量比[（Ｂ）／（Ａ）]は、風味、本発明の効果を享受しやすい点から、０．３以下が好ましく、０．２５以下がより好ましく、０．２以下が更に好ましい。かかる質量比[（Ｂ）／（Ａ）]の範囲としては、好ましくは０．００５～０．３であり、より好ましくは０．０１０～０．３であり、更に好ましくは０．０２～０．３であり、より更に好ましくは０．０４～０．２５であり、殊更に好ましくは０．０９～０．２である。 (a) Green tea extract and (B) metal ions are mixed at a ratio such that the mass ratio of (B) metal ions to (A) non-polymer catechins [(B)/(A)] is 0.005 or more. However, from the viewpoint of suppressing hue change during long-term storage, the mass ratio [(B)/(A)] is preferably 0.010 or more, more preferably 0.02 or more, and 0.04 or more. More preferably, it is 0.09 or more, even more preferably. In addition, from the viewpoint of flavor and ease of enjoying the effects of the present invention, the mass ratio [(B)/(A)] is preferably 0.3 or less, more preferably 0.25 or less, and even more preferably 0.2 or less. preferable. The range of the mass ratio [(B)/(A)] is preferably 0.005 to 0.3, more preferably 0.010 to 0.3, and even more preferably 0.02 to 0. .3, even more preferably from 0.04 to 0.25, even more preferably from 0.09 to 0.2.
水系溶媒としては、例えば、水、有機溶媒水溶液等を挙げることができる。水及び有機溶媒の具体例としては、前述と同様のものを挙げられる。なお、有機溶媒水溶液は、該有機溶媒水溶液中に水が１質量％以上、好ましくは３０質量％以上含まれていればよい。中でも、水系溶媒としては、長期保存したときの色相変化の抑制の観点から、水が好ましく、イオン交換水が更に好ましい。 Examples of the aqueous solvent include water and an aqueous solution of an organic solvent. Specific examples of water and organic solvents include those mentioned above. Note that the organic solvent aqueous solution only needs to contain water in an amount of 1% by mass or more, preferably 30% by mass or more. Among these, as the aqueous solvent, water is preferred, and ion-exchanged water is more preferred, from the viewpoint of suppressing hue change during long-term storage.
水系溶媒の使用量は、長期保存したときの色相変化の抑制の観点から、混合液中に（Ａ）非重合体カテキン類を１質量％以上含有するように調整することが好ましく、２．５質量％以上がより好ましく、５質量％以上がより好ましく、７質量％以上が更に好ましく、１０質量％以上がより更に好ましく、そして同様の観点から、３０質量％以下含有するように調整することが好ましく、２５質量％以下がより好ましく、２０質量％以下が更に好ましく、１５質量％以下がより更に好ましい。かかる混合液中の（Ａ）非重合体カテキン類の含有量の範囲としては、好ましくは１～３０質量％、より好ましくは２．５～２５質量％、更に好ましくは５～２０質量％、より更に好ましくは７～２０質量％、より更に好ましくは１０～１５質量％である。 The amount of the aqueous solvent to be used is preferably adjusted so that the mixed liquid contains 1% by mass or more of (A) non-polymer catechins, from the viewpoint of suppressing hue change during long-term storage. It is more preferably at least 5% by mass, even more preferably at least 7% by mass, even more preferably at least 10% by mass, and from the same point of view, the content can be adjusted to 30% by mass or less. It is preferably 25% by mass or less, more preferably 20% by mass or less, even more preferably 15% by mass or less. The content range of (A) non-polymer catechins in such a mixed solution is preferably 1 to 30% by mass, more preferably 2.5 to 25% by mass, even more preferably 5 to 20% by mass, and more preferably More preferably, it is 7 to 20% by weight, even more preferably 10 to 15% by weight.
（ａ）緑茶抽出物及び（Ｂ）金属イオンのいずれにも溶媒が含まれていない場合には、水系溶媒、（ａ）緑茶抽出物と（Ｂ）金属イオンとを混合する際に、水系溶媒の使用量を、混合液中の（Ａ）非重合体カテキン類の含有量が上記範囲内となるように調整すればよい。また、所定量の水系溶媒と、（ａ）緑茶抽出物と、（Ｂ）金属イオンとを混合し、追加の水系溶媒を添加して混合液中の（Ａ）非重合体カテキン類の含有量が上記範囲内となるように調整することもできる。一方、（ａ）緑茶抽出物及び（Ｂ）金属イオンのいずれか１以上に溶媒が含まれている場合には、当該溶媒量を考慮して混合液中の（Ａ）非重合体カテキン類の含有量が上記範囲内となるように水系溶媒の使用量を調整することができる。なお、（ａ）緑茶抽出物及び（Ｂ）金属イオンのいずれか１以上に含まれている溶媒量によって、混合液中の（Ａ）非重合体カテキン類の含有量が上記要件を満たす場合には、その溶媒中に水が含まれていれば、（ａ）緑茶抽出物と（Ｂ）金属イオンとをそのまま混合すればよい。また、（ａ）緑茶抽出物及び（Ｂ）金属イオンのいずれか１以上に含まれている溶媒量によって、混合液中の（Ａ）非重合体カテキン類の含有量が上記要件を満たす場合において、溶媒中に水が含まれていない場合には、その溶媒を濃縮又は除去した後、水系溶媒を添加して混合液中の（Ａ）非重合体カテキン類の含有量が上記範囲内となるように調整することができる。 When neither (a) green tea extract nor (B) metal ions contain a solvent, an aqueous solvent, an aqueous solvent when mixing (a) green tea extract and (B) metal ions, The amount used may be adjusted so that the content of (A) non-polymer catechins in the mixed liquid falls within the above range. In addition, by mixing a predetermined amount of an aqueous solvent, (a) green tea extract, and (B) metal ions, and adding an additional aqueous solvent, the content of (A) non-polymer catechins in the mixed solution can be increased. can also be adjusted so that it falls within the above range. On the other hand, if any one or more of (a) green tea extract and (B) metal ions contains a solvent, the amount of (A) non-polymer catechins in the mixed solution should be taken into consideration. The amount of the aqueous solvent used can be adjusted so that the content falls within the above range. In addition, depending on the amount of solvent contained in one or more of (a) green tea extract and (B) metal ions, if the content of (A) non-polymer catechins in the mixed solution satisfies the above requirements. If water is contained in the solvent, (a) green tea extract and (B) metal ions may be mixed as they are. In addition, if the content of (A) non-polymer catechins in the mixed solution satisfies the above requirements depending on the amount of solvent contained in one or more of (a) green tea extract and (B) metal ions, If the solvent does not contain water, the solvent is concentrated or removed, and then an aqueous solvent is added so that the content of (A) non-polymer catechins in the mixture falls within the above range. It can be adjusted as follows.
混合方法としては、例えば、撹拌、震盪等の適宜の方法を採用することが可能であり、特に限定されない。
混合温度は、長期保存したときの色相変化の抑制の観点から、１５℃以上が好ましく、１８℃以上がより好ましく、２０℃以上が更に好ましく、また風味の観点から、４０℃以下が好ましく、３５℃以下がより好ましく、３０℃以下が更に好ましい。混合温度の範囲としては、好ましくは１５～４０℃、より好ましくは１８～３５℃、更に好ましくは２０～３０℃である。
混合時間は、長期保存したときの色相変化の抑制の観点から、３分以上が好ましく、５分以上がより好ましく、６分以上が更に好ましく、また生産効率の観点から、９０分以下が好ましく、６０分以下がより好ましく、４０分以下が更に好ましい。混合時間の範囲としては、好ましくは３～９０分、より好ましくは５～６０分、更に好ましくは６～４０分である。
The mixing method is not particularly limited and may be any suitable method such as stirring or shaking.
The mixing temperature is preferably 15°C or higher, more preferably 18°C or higher, even more preferably 20°C or higher, from the viewpoint of suppressing hue change during long-term storage, and preferably 40°C or lower, from the viewpoint of flavor. The temperature is more preferably 30°C or lower, and even more preferably 30°C or lower. The mixing temperature range is preferably 15 to 40°C, more preferably 18 to 35°C, and still more preferably 20 to 30°C.
The mixing time is preferably 3 minutes or more, more preferably 5 minutes or more, even more preferably 6 minutes or more, from the viewpoint of suppressing hue change during long-term storage, and preferably 90 minutes or less from the viewpoint of production efficiency. The time is more preferably 60 minutes or less, and even more preferably 40 minutes or less. The mixing time range is preferably 3 to 90 minutes, more preferably 5 to 60 minutes, and still more preferably 6 to 40 minutes.
次に、混合液を乾燥する。
乾燥方法としては、公知の方法を採用することができる。例えば、噴霧乾燥、ドラム乾燥、凍結乾燥等が挙げられる。乾燥は、粉末緑茶抽出組成物の揮発分が上記範囲内となるように行い、乾燥時間は適宜選択することができる。
また、公知の造粒法により造粒物としてもよい。造粒方法としては、例えば、噴霧造粒、流動層造粒、圧縮造粒、転動造粒、撹拌造粒、押出造粒、粉末被覆造粒等が挙げられる。なお、造粒条件は、造粒方法により適宜選択することができる。
Next, the mixed liquid is dried.
As a drying method, a known method can be adopted. Examples include spray drying, drum drying, freeze drying, and the like. Drying is performed so that the volatile content of the powdered green tea extract composition falls within the above range, and the drying time can be selected as appropriate.
Alternatively, it may be made into a granulated product using a known granulation method. Examples of the granulation method include spray granulation, fluidized bed granulation, compression granulation, rolling granulation, stirring granulation, extrusion granulation, powder coating granulation, and the like. Note that the granulation conditions can be appropriately selected depending on the granulation method.
上記の実施形態に関し、以下に本発明の好ましい態様を更に開示する。
＜１＞ 次の成分（Ａ）及び（Ｂ）：
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０～９５質量％であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．０１０～０．３である、粉末緑茶抽出組成物。
Regarding the above embodiments, preferred aspects of the present invention will be further disclosed below.
<1> The following components (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
A powder in which the content of component (A) is 40 to 95% by mass, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.010 to 0.3. Green tea extract composition.
＜２＞ 次の成分（Ａ）及び（Ｂ）：
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０～９５質量％であり、
成分（Ｂ）の含有量が１．０～１２質量％であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上である、
粉末緑茶抽出組成物。
<2> The following components (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
The content of component (A) is 40 to 95% by mass,
The content of component (B) is 1.0 to 12% by mass, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.005 or more.
Powdered green tea extract composition.
＜３＞ 次の成分（Ａ）及び（Ｂ）：
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０質量％以上であり、
成分（Ｂ）の含有量が１．０～１２質量％であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．０１０～０．３である、粉末緑茶抽出組成物。
<3> The following components (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
The content of component (A) is 40% by mass or more,
The content of component (B) is 1.0 to 12% by mass, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.010 to 0.3. , powdered green tea extract composition.
＜４＞ 次の成分（Ａ）及び（Ｂ）：
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０～９５質量％であり、
成分（Ｂ）の含有量が１．０～１２質量％であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．０１０～０．３である、粉末緑茶抽出組成物。
<4> The following components (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
The content of component (A) is 40 to 95% by mass,
The content of component (B) is 1.0 to 12% by mass, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.010 to 0.3. , powdered green tea extract composition.
＜５＞ 成分（Ｂ）として１価の金属イオン及び２価の金属イオンから選ばれる１種又は２種以上を含む、前記＜１＞～＜４＞のいずれか一に記載の粉末緑茶抽出組成物。
＜６＞ 成分（Ｂ）としてナトリウムイオン、カリウムイオン及びカルシウムイオンから選択される１種又は２種以上を含む、前記＜１＞～＜４＞のいずれか一に記載の粉末緑茶抽出組成物。
＜７＞ 揮発分が０．６～５．０質量％である、前記＜１＞～＜４＞のいずれか一に記載の粉末緑茶抽出組成物。
＜８＞ 平均粒子径が１～１０００μｍである、前記＜１＞～＜４＞のいずれか一に記載の粉末緑茶抽出組成物。
<5> The powdered green tea extract composition according to any one of <1> to <4> above, containing one or more selected from monovalent metal ions and divalent metal ions as component (B). thing.
<6> The powdered green tea extract composition according to any one of <1> to <4>, which contains one or more selected from sodium ions, potassium ions, and calcium ions as component (B).
<7> The powdered green tea extract composition according to any one of <1> to <4>, wherein the volatile content is 0.6 to 5.0% by mass.
<8> The powdered green tea extract composition according to any one of <1> to <4> above, having an average particle diameter of 1 to 1000 μm.
＜９＞ 固形分中の（Ａ）非重合体カテキン類の含有量が４０～９５質量％である緑茶抽出物と、（Ｂ）金属イオンとを、成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．０１０～０．３となるように水系溶媒の存在下に混合した後、当該混合液を乾燥する工程を含む、粉末緑茶抽出組成物の製造方法。 <9> A green tea extract having a content of (A) non-polymer catechins in the solid content of 40 to 95% by mass, and (B) metal ions, in proportion to the mass of component (B) relative to component (A). A method for producing a powdered green tea extract composition, comprising the step of mixing in the presence of an aqueous solvent so that the ratio [(B)/(A)] is 0.010 to 0.3, and then drying the mixed solution. .
＜１０＞ 固形分中の（Ａ）非重合体カテキン類の含有量が４０～９５質量％である緑茶抽出物と、（Ｂ）金属イオン０．２～１４質量％とを、成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上となるように水系溶媒の存在下に混合した後、当該混合液を乾燥する工程を含む、粉末緑茶抽出組成物の製造方法。 <10> Component (A) is a green tea extract in which the content of (A) non-polymer catechins in the solid content is 40 to 95% by mass, and (B) 0.2 to 14% by mass of metal ions. A powdered green tea extract composition comprising the step of mixing in the presence of an aqueous solvent such that the mass ratio of component (B) to the component (B) is 0.005 or more, and then drying the mixed solution. How things are manufactured.
＜１１＞ 水系溶媒が、水又は有機溶媒水溶液である、前記＜９＞又は＜１０＞記載の粉末緑茶抽出組成物の製造方法。
＜１２＞ 水系溶媒の使用量が、混合液中に成分（Ａ）が１～３０質量％となる量である、前記＜９＞又は＜１０＞記載の粉末緑茶抽出組成物の製造方法。
＜１３＞ 混合時間が、３～９０分である、前記＜９＞又は＜１０＞記載の粉末緑茶抽出組成物の製造方法。
<11> The method for producing a powdered green tea extract composition according to <9> or <10> above, wherein the aqueous solvent is water or an aqueous organic solvent solution.
<12> The method for producing a powdered green tea extract composition according to <9> or <10>, wherein the amount of the aqueous solvent used is such that component (A) is 1 to 30% by mass in the mixed liquid.
<13> The method for producing a powdered green tea extract composition according to <9> or <10> above, wherein the mixing time is 3 to 90 minutes.
１．非重合体カテキン類の分析
純水で溶解希釈した試料を、島津製作所製、高速液体クロマトグラフ（型式ＳＣＬ－１０ＡＶＰ）を用い、オクタデシル基導入液体クロマトグラフ用パックドカラム（Ｌ－カラムＴＭ ＯＤＳ、４．６ｍｍφ×２５０ｍｍ：財団法人 化学物質評価研究機構製）を装着し、カラム温度３５℃でグラジエント法により測定した。移動相Ａ液は酢酸を０．１ｍｏｌ／Ｌ含有する蒸留水溶液、Ｂ液は酢酸を０．１ｍｏｌ／Ｌ含有するアセトニトリル溶液とし、流速は１ｍＬ／分、試料注入量は１０μＬ、ＵＶ検出器波長は２８０ｎｍの条件で行った。なお、グラジエント条件は以下の通りである。
1. Analysis of non-polymer catechins A sample dissolved and diluted with pure water was analyzed using a high-performance liquid chromatograph (model SCL-10AVP) manufactured by Shimadzu Corporation, using an octadecyl group-introduced packed column for liquid chromatography (L-column TM ODS, 4). .6 mmφ x 250 mm (manufactured by the Japan Chemical Evaluation and Research Institute) was installed, and the measurement was carried out using a gradient method at a column temperature of 35°C. Mobile phase A solution was a distilled aqueous solution containing 0.1 mol/L of acetic acid, B solution was an acetonitrile solution containing 0.1 mol/L of acetic acid, the flow rate was 1 mL/min, the sample injection amount was 10 μL, and the UV detector wavelength was The experiment was carried out under the condition of 280 nm. Note that the gradient conditions are as follows.
濃度勾配条件（体積％）
時間 Ａ液濃度 Ｂ液濃度
０分 ９７％ ３％
５分 ９７％ ３％
３７分 ８０％ ２０％
４３分 ８０％ ２０％
４３．５分 ０％ １００％
４８．５分 ０％ １００％
４９分 ９７％ ３％
６０分 ９７％ ３％
Concentration gradient conditions (volume%)
Time A liquid concentration B liquid concentration 0 minutes 97% 3%
5 minutes 97% 3%
37 minutes 80% 20%
43 minutes 80% 20%
43.5 minutes 0% 100%
48.5 minutes 0% 100%
49 minutes 97% 3%
60 minutes 97% 3%
２．金属イオンの分析
試料２ｇに１０％塩酸５ｍＬを加え、水浴上で蒸発乾固する。さらに１０％塩酸５ｍＬを加え、加温した後、全量をメスフラスコに濾過し、水で定容する。１％塩酸を用いて、検量線の範囲内に入るように、適当な濃度に希釈し、２００００ｐｐｍのストロンチウム液２．５ｍＬを加え、定容したものを試験溶液とする。原子吸光光度計を用いて、試験溶液の吸光度を測定し、あらかじめ作成した検量線から金属の定量を行う。
2. Analysis of metal ions Add 5 mL of 10% hydrochloric acid to 2 g of sample and evaporate to dryness on a water bath. Further, 5 mL of 10% hydrochloric acid is added, and after heating, the entire amount is filtered into a volumetric flask, and the volume is adjusted with water. Dilute with 1% hydrochloric acid to an appropriate concentration so that it falls within the range of the calibration curve, add 2.5 mL of 20,000 ppm strontium solution, and make the solution to a fixed volume, which is used as the test solution. Measure the absorbance of the test solution using an atomic absorption spectrophotometer, and quantify the metal using a calibration curve prepared in advance.
・原子吸光光度計:Ｚ－２３１０（ＨＩＴＡＣＨＩ製）
・フレーム：空気－アセチレン
・測定波長：５８９．０ｎｍ（ナトリウム）、７６６．５ｎｍ（カリウム）、４２２．７ｎｍ（カルシウム）
・Atomic absorption photometer: Z-2310 (manufactured by HITACHI)
・Frame: Air-acetylene ・Measurement wavelength: 589.0 nm (sodium), 766.5 nm (potassium), 422.7 nm (calcium)
３．色相変化の評価
分光光度計（形式Color Meter ZE-2000、日本電色工業社製）を使用し、粉末試料を３０φの円形セルに入れてＬ*ａ*ｂ*表色系のａ*値を反射測定した。３７℃／５０％ＲＨの雰囲気下で８週間保存した後の粉末緑茶抽出組成物のａ*値と、製造直後の粉末緑茶抽出組成物のａ1
*値との差分の絶対値Δａ*（｜ａ*－ａ1
*｜）を求めた。
3. Evaluation of hue change Using a spectrophotometer (model Color Meter ZE-2000, manufactured by Nippon Denshoku Kogyo Co., Ltd.), place the powder sample in a 30φ circular cell and measure the a * value of the L * a * b * color system. Reflection measurements were taken. Absolute value Δa * of the difference between the a * value of the powdered green tea extract composition after storage for 8 weeks in an atmosphere of 37° C./50% RH and the a 1 * value of the powdered green tea extract composition immediately after production. a * - a 1 * |) was calculated.
製造例１
〔緑茶抽出物の製造〕
ケニア産の乾燥緑茶葉３ｋｇを９０℃の熱水３０Ｌにて３０分間抽出後、濾過し、得られた緑茶抽出液をスプレードライにて乾燥し、粉末状の緑茶抽出物（Ｉ）を得た。緑茶抽出物（Ｉ）は、（Ａ）非重合体カテキン類の含有量が３８．３質量％であった。
９２．２質量％のエタノール水溶液８００ｇに、得られた緑茶抽出物（Ｉ）２００ｇを投入し、次いで酸性白土（ミズカエース＃６００、水澤化学社製）を１００ｇ、ろ過助剤（ソルカフロック、栗田工業社製）１２．６ｇを添加した。その後、２５℃にて６時間撹拌した。次に、プレコート剤を堆積させた２号濾紙にてろ過した。次に、ろ過液をエタノール濃度が６０質量％になるように加水した後、遠心分離を行い、上澄み液を回収した。次に、上澄み液を活性炭（ＳＧ－Ｋ、フタムラ化学製）２４ｇが充填されたカラムに通液し、回収液をトッピングしエタノールを留去することで、緑茶抽出物（II）を得た。得られた緑茶抽出物（II）は、（Ａ）非重合体カテキン類の含有量が１４．８質量％であり、固形分中の（Ａ）非重合体カテキン類の含有量が６２．５質量％であった。
Manufacturing example 1
[Manufacture of green tea extract]
3 kg of dried green tea leaves from Kenya were extracted with 30 L of hot water at 90°C for 30 minutes, filtered, and the obtained green tea extract was dried by spray drying to obtain a powdered green tea extract (I). . The content of (A) non-polymer catechins in the green tea extract (I) was 38.3% by mass.
200 g of the obtained green tea extract (I) was added to 800 g of a 92.2% by mass ethanol aqueous solution, and then 100 g of acid clay (Mizuka Ace #600, manufactured by Mizusawa Chemical Co., Ltd.) and a filter aid (Solka Floc, Kurita Water Industries, Ltd.) were added. 12.6 g (manufactured by Seiko Co., Ltd.) was added. Thereafter, the mixture was stirred at 25°C for 6 hours. Next, it was filtered using a No. 2 filter paper on which a precoating agent was deposited. Next, water was added to the filtrate so that the ethanol concentration was 60% by mass, followed by centrifugation and the supernatant was collected. Next, the supernatant liquid was passed through a column filled with 24 g of activated carbon (SG-K, manufactured by Futamura Chemical Co., Ltd.), the recovered liquid was topped with the ethanol, and the ethanol was distilled off to obtain green tea extract (II). The obtained green tea extract (II) has a content of (A) non-polymer catechins of 14.8% by mass, and a content of (A) non-polymer catechins in the solid content of 62.5% by mass. It was mass%.
実施例１～６
緑茶抽出物（II）と、表１に示す金属塩とを、（Ａ）非重合体カテキン類に対する（Ｂ）金属イオンの質量比[（Ｂ）／（Ａ）]が表１に示す割合となるように混合した後、２５℃にて３０分間撹拌した。その後、混合液をスプレードライにて、揮発分が表１に示す割合となるまで乾燥し、粉末緑茶抽出組成物を得た。得られた粉末緑茶抽出組成物について分析を行った。また、粉末緑茶抽出組成物２ｇを用い、色相変化の評価を行った。その結果を表１に示す。
Examples 1 to 6
The green tea extract (II) and the metal salts shown in Table 1 were mixed at a mass ratio of (B) metal ions to (A) non-polymer catechins [(B)/(A)] shown in Table 1. After mixing the mixture, the mixture was stirred at 25° C. for 30 minutes. Thereafter, the mixed liquid was dried by spray drying until the volatile content reached the ratio shown in Table 1, to obtain a powdered green tea extract composition. The obtained powdered green tea extract composition was analyzed. Further, hue change was evaluated using 2 g of the powdered green tea extract composition. The results are shown in Table 1.
比較例１
金属イオンを添加しなかったこと以外は、実施例１と同様の操作により粉末緑茶抽出組成物を得た。得られた粉末緑茶抽出組成物について、実施例１と同様に分析、色相変化の評価を行った。その結果を表１に示す。
Comparative example 1
A powdered green tea extract composition was obtained in the same manner as in Example 1 except that no metal ions were added. The obtained powdered green tea extract composition was analyzed and evaluated for hue change in the same manner as in Example 1. The results are shown in Table 1.
表１から、（Ａ）非重合体カテキン類の含有量を４０質量％以上とした際に、（Ａ）非重合体カテキン類に対する（Ｂ）金属イオンの質量比[（Ｂ）／（Ａ）]を０．００５以上に制御することで、長期保存したときの色相変化が抑制された粉末緑茶抽出組成物が得られることがわかる。 From Table 1, when the content of (A) non-polymer catechins is 40% by mass or more, the mass ratio of (B) metal ions to (A) non-polymer catechins [(B)/(A) ] It can be seen that by controlling 0.005 or more, a powdered green tea extract composition with suppressed hue change during long-term storage can be obtained.
Claims (8)
（Ａ）非重合体カテキン類、及び
（Ｂ）金属イオン
を含有し、
成分（Ａ）の含有量が４０質量％以上であり、かつ
成分（Ａ）に対する成分（Ｂ）の質量比[（Ｂ）／（Ａ）]が０．００５以上である、
粉末緑茶抽出組成物。 The following ingredients (A) and (B):
Contains (A) non-polymer catechins and (B) metal ions,
The content of component (A) is 40% by mass or more, and the mass ratio of component (B) to component (A) [(B)/(A)] is 0.005 or more.
Powdered green tea extract composition.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007159541A (en) | 2005-12-17 | 2007-06-28 | Pharma Foods International Co Ltd | High-concentration solution containing catechins and method for producing the same |
| JP2009201384A (en) | 2008-02-26 | 2009-09-10 | Ito En Ltd | Green tea extract, its use and production method |
| JP2012115229A (en) | 2010-12-03 | 2012-06-21 | Kao Corp | Purified tea extract |
| JP2014140354A (en) | 2012-12-28 | 2014-08-07 | Kao Corp | Process for producing tea extract |
| JP2015109829A (en) | 2013-10-29 | 2015-06-18 | 花王株式会社 | Green tea extract composition |
| JP2016208960A (en) | 2015-04-28 | 2016-12-15 | 花王株式会社 | Green tea extraction composition |
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| JPH09322710A (en) * | 1996-06-03 | 1997-12-16 | Minato Seiyaku Kk | Production of tea extract containing catechins |
| JPH11266841A (en) * | 1998-03-19 | 1999-10-05 | Fukujuen:Kk | Barley-tea bag containing catechin-enriched green tea |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007159541A (en) | 2005-12-17 | 2007-06-28 | Pharma Foods International Co Ltd | High-concentration solution containing catechins and method for producing the same |
| JP2009201384A (en) | 2008-02-26 | 2009-09-10 | Ito En Ltd | Green tea extract, its use and production method |
| JP2012115229A (en) | 2010-12-03 | 2012-06-21 | Kao Corp | Purified tea extract |
| JP2014140354A (en) | 2012-12-28 | 2014-08-07 | Kao Corp | Process for producing tea extract |
| JP2015109829A (en) | 2013-10-29 | 2015-06-18 | 花王株式会社 | Green tea extract composition |
| JP2016208960A (en) | 2015-04-28 | 2016-12-15 | 花王株式会社 | Green tea extraction composition |
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