JP7384453B2 - 3d堆積用バイオインク - Google Patents
3d堆積用バイオインク Download PDFInfo
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- JP7384453B2 JP7384453B2 JP2021521336A JP2021521336A JP7384453B2 JP 7384453 B2 JP7384453 B2 JP 7384453B2 JP 2021521336 A JP2021521336 A JP 2021521336A JP 2021521336 A JP2021521336 A JP 2021521336A JP 7384453 B2 JP7384453 B2 JP 7384453B2
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Description
本発明は、国立衛生研究所(NIH)によって付与されたHL137204の下で政府の支援を受けて成された。政府は本発明に関して一定の権利を有する。
本開示は、構造体の三次元(3D)印刷に用いることができるバイオインク用の組成物を記載する。心臓組織工学の目標は、生理学的および解剖学的構造の理解を助けるインビトロ(in vitro)モデルシステムを作製することである。例えば、症状を緩和しかつ生存能力のアウトプットを改善する治療薬を創製するために、発達、成長および疾患に関して心臓をより詳細に理解することが望ましい場合がある。当該分野は、多能性幹細胞を効率的に利用して高効率で全ての心細胞型を誘導し、典型的にはヒトの筋肉を模倣した、可撓性基板(フレキシブルポスト)により担持されたマイクロスケールの組織片の形をとっている、しばしば「ミクロ組織」と称されるモデル系の開発へと移行した。このような組織片は、医薬品産業への恩恵を伴う薬物の試験に非常に有望であるが、組織片はまた、心室のような構造体のアーキテクチャと機能性出力を正確に再現する能力を欠いている。これは、組織の性能を動物またはヒトの心臓の性能と直接比較することは不可能であり、そのような性能は心室の圧力と容積の変化を測定することによって評価できるために、重大な制限因子である。さらに、疾患を伴う心臓のリモデリングは、複雑な流動プロファイルと、関連する心室に加わる力の刺激とその応答により、実質的に影響を受ける。
Claims (15)
- 10~15重量/容積%のゼラチンメタクリレートと、0.1~1.0重量/容積%のコラーゲンメタクリレートとを含むバイオインク組成物。
- フェニル-2,4,6-トリメチルベンゾイルホスフィン酸リチウム塩をさらに含む、請求項1に記載のバイオインク組成物。
- mTeSR培地;酢酸;および水酸化ナトリウム(NaOH)を含む溶媒をさらに含む、請求項2に記載のバイオインク組成物。
- 前記溶媒が74重量/容積%のmTeSR 培地;20重量/容積%の20 mM酢酸;および1重量/容積%の1M NaOHを含む、請求項3に記載のバイオインク組成物。
- 前記バイオインク組成物が、10重量/容積%のゼラチンメタクリレート;0.25重量/容積%のコラーゲンメタクリレート;および0.5重量/容積%のフェニル-2,4,6-トリメチルベンゾイルホスフィン酸リチウム塩を含む、請求項1~4のいずれか一項に記載のバイオインク組成物。
- フィブロネクチンまたはラミニンの少なくとも1つをさらに含む、請求項1~5のいずれか一項に記載のバイオインク組成物。
- 前記組成物が、100ミリグラム/ミリリットル(mg/mL)のコラーゲンメタクリレート;2.5 mg/mLのゼラチンメタクリレート;5 mg/mLのフェニル-2,4,6-トリメチルベンゾイルホスフィン酸リチウム塩;93.8マイクログラム/ミリリットル(μg/mL)のフィブロネクチン;および93.8μg/mLのラミニンを含む、請求項1~6のいずれか一項に記載のバイオインク組成物。
- ヒト人工多能性幹細胞をさらに含む、請求項1~7のいずれか一項に記載のバイオインク組成物。
- 前記ヒト人工多能性幹細胞が、ミオシン重鎖(MHC)のもとでサイクリンD2(CCND2)を過剰発現するヒト心臓線維芽細胞由来の人工多能性幹細胞を含む、請求項8のバイオインク組成物。
- 前記ヒト人工多能性幹細胞が心筋細胞前駆体を含む、請求項8のバイオインク組成物。
- 心筋細胞をさらに含む、請求項1~10のいずれか一項のバイオインク組成物。
- 前記バイオインク組成物が、心筋細胞へのヒト人工多能性幹細胞の分化を促進するように構成される、請求項1~11のいずれか一項に記載のバイオインク組成物。
- 請求項1~12のいずれか一項のバイオインク組成物を使って三次元構造体を印刷することを含む方法。
- 請求項1~12のいずれか一項のバイオインク組成物を使って三次元構造体を印刷することが、前記バイオインクを使って三次元構造体を印刷して少なくとも1つの室を構築することを含む、請求項13の方法。
- 前記三次元構造体が、ヒト人工多能性幹細胞を含み、そして前記方法が、Wnt/β-カテニン経路を調節することによりヒト人工多能性幹細胞を心筋細胞へと分化するように誘導することをさらに含む、請求項13~14のいずれか一項に記載の方法。
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PCT/US2019/057009 WO2020081982A1 (en) | 2018-10-18 | 2019-10-18 | Bioink for 3d deposition |
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CN111388750B (zh) * | 2020-04-30 | 2022-09-13 | 深圳先进技术研究院 | 生物墨水、小口径管状结构支架及其制备方法和应用 |
US20220016419A1 (en) * | 2020-07-16 | 2022-01-20 | Biosense Webster (Israel) Ltd. | Apparatus for testing a cardiac catheter utilizing live cardiac cells |
US11918703B2 (en) * | 2020-08-13 | 2024-03-05 | Universidad De Los Andes | Extrudable photocrosslinkable hydrogel and method for its preparation |
WO2022073090A1 (pt) | 2020-10-06 | 2022-04-14 | Janaina De Andrea Dernowsek Ltda | Método para a produção de uma composição de proteínas de matriz extracelular e produto obtido por tal método |
KR102475487B1 (ko) * | 2020-10-12 | 2022-12-08 | 고려대학교 산학협력단 | 심장 이식용 시트 및 이의 제조방법 |
CN112852724B (zh) * | 2021-01-05 | 2022-09-06 | 首玺(广州)医疗科技有限责任公司 | 一种含干细胞活性因子的美容组合物及其制备方法和应用 |
WO2022261534A2 (en) * | 2021-06-11 | 2022-12-15 | University Of Maryland, Baltimore | Three-dimensional cell-laden bioink scaffolds and methods of making the same under cryogenic conditions for tissue engineering |
CN113750292B (zh) * | 2021-09-30 | 2022-10-25 | 华南理工大学 | 一种用于3d打印角膜修复材料的生物墨水及制备方法、角膜修复材料的制备方法 |
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