JP7376758B1 - skin beauty method - Google Patents

Abstract

[Problem] To obtain a sufficient youthful appearance effect and improve user satisfaction. [Solution] A liquid storage part that stores a spray liquid containing an active ingredient, a spray port that sprays the spray liquid stored in the liquid storage part, a gas supply part that supplies gas, and the gas supply. A multi-component skin penetration system comprising a spray control section that instantaneously supplies gas supplied from the spray port to the spray port and causes the spray liquid to be sprayed together with the gas from the spray port, and contains NMN. A first atomizing liquid is atomized and a second atomizing liquid containing a human stem cell culture solution is atomized. [Selection diagram] Figure 3

Description

The present invention relates to a skin beautifying method for beautifying the skin.

Conventionally, as a skin beauty composition that improves wrinkles and has an excellent usability, a skin beauty composition characterized by containing an acetic acid extract of onion scales and baking soda, and a skin beauty method for applying the same (however, , excluding medical practice) has been proposed (see Patent Document 1).
In this skin beauty method, onion scale acetic acid extract alone has wrinkle improvement, moisturizing effect, and skin beautification effect (improvement of texture), but by also containing baking soda, it can improve skin quality in a shorter period of time. It is said that it has been found to have cosmetic effects (particularly wrinkle improvement).
Here, in the beauty industry, there is a demand for further effects, and a younger-looking effect is desired. In other words, in addition to wrinkle-improving, moisturizing, and skin-beautifying effects, there is also a demand for a lifting effect that improves facial sagging and wrinkles.
However, the above-mentioned beauty methods have not been able to provide a lift-up effect.

Patent No. 6973740

In view of the above-mentioned problems, it is an object of the present invention to provide a sufficient youthful appearance effect and improve user satisfaction.

This invention applies a first composition containing either a first active ingredient in liquid or powder form consisting of a human stem cell culture solution and a second active ingredient in liquid or powder form consisting of NMN. applying a second composition containing the other of the first active ingredient and the second active ingredient; A skin beauty method in which the other active ingredient contained in the first composition is not included, and the second composition does not contain the one active ingredient contained in the first composition (however, it is not a medical procedure). ).

According to the present invention, it is possible to provide a skin beauty method that provides a sufficiently youthful appearance effect.

System configuration diagram of a multi-component skin penetration system. FIG. 1 is a block diagram showing the configuration of a multi-component skin penetration system. Flowchart showing the operation of the multi-component skin penetration system. An explanatory diagram of before and after using a multi-component skin penetration system.

In this invention, a first composition containing a first active ingredient and a second composition containing a second active ingredient are applied to the skin either separately or in combination. One of the first active ingredients and the second active ingredient is a human stem cell culture medium, and the other is NMN. In addition, as a method of application, the first penetrant consisting of the first active ingredient and the second penetrating agent consisting of the second active ingredient are adjusted individually or all together with a conditioning agent such as purified water, and then sprayed. It is preferable to use a liquid for application (liquid for application) and spray it onto the skin using a spray device that performs spraying. The skin here refers to all skin on the body, including facial skin, neck skin, décolleté skin, extremity skin, chest skin, abdominal skin, back skin, genital skin, and head skin. refers to
An embodiment of the present invention will be described below with reference to the drawings.

FIG. 1 is a block diagram showing the system configuration of a multi-component skin penetration system 1.
The multi-component skin penetration system 1 includes a first penetrant 2, a second penetrant 3, a conditioning agent 4, and a spray device 5.

The first penetrant 2 is an agent made of a first active ingredient that penetrates into the skin, and is made of liquid or powder.
The second penetrant 3 is an agent made of a second active ingredient that penetrates into the skin, and is made of liquid or powder.

In this example, one of the first penetrant 2 and the second penetrant 3 is NMN powder, and the other is a human stem cell culture solution.
NMN powder is a powder of nicotinamide mononucleotide (NMN), which is collected from breast milk, edamame, broccoli, avocado, tomato, and other living things and is formed by pulverizing it through granulation processing such as micro-granulation processing. .

A human stem cell culture solution is a supernatant obtained when stem cells collected from humans are cultured in a culture solution. Human stem cell culture supernatant includes human umbilical cord stem cell culture supernatant obtained from human umbilical cord, human umbilical cord blood stem cell culture supernatant obtained from human umbilical cord blood, and human adipose stem cell culture supernatant obtained from human fat. A human bone marrow stem cell culture supernatant obtained from human bone marrow fluid, and a supernatant obtained by culturing other human cells can be used.

Conditioner 4 can be purified water or physiological saline. As the purified water, normal purified water or sterilized purified water obtained by sterilizing purified water can be used. As the physiological saline, an appropriate saline can be used, such as saline for injection, which is purified water that has been sterilized by filtration or distillation, and further pyrogenic substances have been removed.

The first penetrating agent 2 is adjusted with the adjusting agent 4 and used as a first spraying liquid (first coating liquid). Similarly, the second penetrating agent 3 is adjusted with the adjusting agent 4 and used as a second spraying liquid (second coating liquid). Note that it is also possible to mix the first penetrant 2 and the second penetrant 3 and adjust the mixture with the conditioning agent 4 to use the mixture as one integrated spraying liquid (integrated coating liquid).

The atomizing liquid (the first atomizing liquid, the second atomizing liquid, or the integrated atomizing liquid) prepared in this way can have a viscosity of 200 mPa·s or less, and may have a viscosity of 100 mPa·s. The following are preferable, those with a viscosity of 50 mPa·s or less are more preferable, those with a viscosity of 10 mPa·s or less are more preferable, those with a viscosity of 5 Pa·s or less are still more preferable, and those with a viscosity of 3 mPa·s or less are preferable. is more preferable, those with a viscosity of 2 mPa·s or less are even more preferable, and those with a viscosity of 1 mPa·s or less are suitable. Thereby, it is possible to prevent clogging inside when spraying from the spray port 12a of the spray device 5, which will be described later.

The spray device 5 is a device that sprays pressurized liquid onto an object (in this example, human skin) via a nozzle, and will be described in detail below.

The spray device 5 includes a main body 30 that sends out air, a conduit 20 connected at one end to the main body 30, and a hand piece 10 connected to the other end of the conduit 20 and operated by a practitioner.

The handpiece 10 is a longitudinal unit that can be held in the hands of a practitioner, and has a spray cover 11 provided at its tip and the other end of a conduit 20 connected to its rear end. A liquid input section 15 is provided on the top surface of the hand piece 10 from the center to the distal end side, into which a spraying liquid prepared by adjusting the first penetrant 2 and the second penetrant 3 with a regulating agent 4 is introduced. The liquid input section 15 has a liquid input hole 15b provided in the lid on the top surface, and a liquid storage section 15a provided inside the hand piece 10. The liquid storage section 15a is a sterile chamber, and a spraying liquid is introduced into the liquid storage section 15a from a liquid injection hole 15b using a syringe or the like. On the rear end side from the center of the top surface of the handpiece 10, there is a display section 19a (see FIG. 2) that displays the current spray setting, a switching button 19b (see FIG. 2) that switches the spray setting of the spray liquid, and a spray button 19b (see FIG. 2) that displays the current spray setting. A setting section 19 is provided, which includes an operation button 19c (see FIG. 2) for switching between starting and stopping spraying of the liquid for use, and a control section 19d for executing various controls. A spray amount adjustment dial 18 is provided on the right side of the handpiece 10 (the front in FIG. 1) to adjust the amount of spray liquid in one spray. ) is provided with a manual spray button 16 that sprays once when pressed when the spray setting is manual spray.

The conduit 20 is provided with a pipe 20a (see FIG. 2) for supplying gas (air) to the handpiece 10 and an electric wire 20b (see FIG. 2) for supplying electric power to the handpiece 10. This conduit 20 can be freely curved, and the practitioner can freely move the handpiece 10 and use it for treatment while leaving the main body 30 in place.

The main body 30 has an operation start button 30a for switching start/stop of operation provided on the front, a power cord (not shown) for connecting to a household power source provided on the back, and a conduit on the side. One end of 20 is connected.

FIG. 2 is a block diagram showing the configuration of the multi-component skin penetration system 1.
Inside the hand piece 10, a spray tip 12 is provided on the distal end side, and a transparent, substantially cylindrical spray cover 11 is attached to the outer periphery of the spray tip 12. The spray cover 11 has a cylindrical shape with a tip cut perpendicularly to the axis of the cylinder, and gas vent spaces 11a are provided at the tip in two opposing positions on the left and right sides. That is, most of the tip is a circular ring-shaped tip on a plane, and at least a part of it is a recessed portion that becomes the gas vent space 11a. In the illustrated example, the gas vent space 11a is formed by a concave cutout groove in which the open end of the spray cover 11 is partially switched.

A spray port 12a for spraying a spray liquid is provided at the center of the spray tip 12, which is the tip of the hand piece 10, and a gas discharge portion 14 is provided at the back of the spray port 12a. A spray gas chamber 14b is provided in the gas discharge portion 14, and the spray gas chamber 14b communicates with the spray port 12a. The air port 14a and the spray port 12a at the tip of the spray gas chamber 14b are provided to face each other. A spray liquid chamber 13 is provided on the outer periphery between the air port 14a and the spray port 12a, and this spray liquid chamber 13 communicates with the spray port 12a. The spray liquid chamber 13 is a sterile chamber, and communicates with a liquid storage section 15a containing a spray liquid. Further, the discharge speed of the atomizing liquid and gas discharged from the gas discharge part 14 through the spray nozzle 12a can be 100 km/h or more, preferably 500 km/h or more, and preferably 1000 km/h or more. be. Moreover, the upper limit of this release speed can be 5000 km/h or less, preferably 3000 km/h or less, and preferably 2000 km/h or less. By releasing the compressed gas at high speed in a short period of time in this way, the liquid for spraying can be released in nano form. By spraying (coating) the nanoparticle in this way, the spray liquid can penetrate more deeply into the skin.

The spray tip 12 having the spray nozzle 12a is covered with a spray cover 11 on its outer periphery and on the spray tip side. Therefore, the spray liquid and gas sprayed from the spray port 12a are vigorously sprayed (sprayed) onto the skin to be sprayed while being confined within the spray cover 11, and the excess gas (including the spray liquid) is turned into gas. It is discharged to the outside from the extraction space 11a.

A solenoid 17 is provided inside the handpiece 10 and downstream of the gas discharge section 14 . This solenoid 17 is provided in the middle of a pipe 20a that supplies gas to the atomized gas chamber 14b of the gas discharge section 14, and switches between passing and blocking the gas through the pipe 20a. This solenoid 17 and a control section 19d that controls it function as a spray control section.

Furthermore, a control section 19d (see FIG. 1) is provided inside the handpiece 10. In response to the operation of the switching button 19b, the control unit 19d changes the spray settings of the spray liquid to manual spray, which performs spraying once when the manual spray button 16 is pressed, and a slow first time interval ( First intermittent spraying that continuously performs instantaneous short-time spraying (for example, 0.1 seconds) at intervals of 1.5 seconds, and second intermittent spraying that is shorter than the first intermittent spraying (for example, every 1.0 seconds). ), a second intermittent spray that continuously performs short-time spraying (for example, 0.1 seconds, etc.); Switch to one of the third intermittent sprays that are performed continuously (e.g., 1 second). Incidentally, the instantaneous short-time spray can be a spray for 1 second or less, preferably a spray for 0.5 seconds or less, more preferably a spray for 0.3 seconds or less, and 0.5 seconds or less. It is more preferable to spray for 2 seconds or less, and preferably for 0.1 seconds or less. Further, the lower limit of instantaneous short-time spraying can be set to 0.001 seconds or more. It is preferable to set it as 0.01 second or more, and it is preferable to set it as 0.05 second or more.

In addition, when the manual spray button 16 is pressed when the spray setting is manual spray (or when the timing for spraying with the first intermittent spray, the second intermittent spray, or the third intermittent spray is reached), the control unit 19d sprays the spray. The solenoid 17 is turned ON for an instantaneous predetermined period of time (for example, 0.1 seconds) to open the pipe 20a and allow the gas to pass through, and then the solenoid 17 is turned OFF and the pipe 20a is closed to allow the gas to pass through. Execute spray control to prevent By controlling the spray in this way, negative pressure is generated in the spray liquid chamber 13 at the moment the solenoid 17 is turned off, and the spray liquid in the liquid storage part 15a is supplied to the spray liquid chamber 13, and the next When the solenoid 17 is turned on, the gas is instantaneously supplied, and the atomizing liquid is atomized from the atomizing port 12a together with the gas.

The control unit 19d also performs an operation of displaying the current spray settings and an error code in the case of an error on the display unit 19a.
Further, the control unit 19d executes these operations after the operation is turned on by the operation button 19c, and stops and does not execute each operation when the operation is turned off by the operation button 19c. Note that when the solenoid 17 is in an OFF state, the gas supplied from the pipe 20a flows to an exhaust path (not shown).

These manual spray button 16, solenoid 17, display section 19a, switching button 19b, actuation button 19c, and control section 19d are supplied with electric power from the main body 30 through an electric wire 20b.

A spray amount adjustment section 18 is provided in the hand piece 20 at a stage downstream from the solenoid 17. This spray amount adjustment section 18 acts on the pipe 20a to adjust the amount of gas supplied when the solenoid 17 is turned on. This makes it possible to adjust the amount of gas and liquid to be sprayed in one spray. The spray amount adjusting section 18 may be configured to adjust the spray amount by changing the opening size of the spray port 12a. In this case, the spray range and spray intensity can also be adjusted.

The main body 30 is provided with a control section 31, a power supply section 32, and a gas supply section 33.
The control unit 31 controls the supply of gas by the gas supply unit 33. Specifically, when the operation start button 30a (see FIG. 1) is pressed to turn on the power, the gas supply unit 33 is driven to start supplying gas, and power is supplied from the electric wire 20b to the unit inside the handpiece 10. When the supply is started and the operation start button 30a (see FIG. 1) is pressed again to turn off the power, the gas supply and power supply are stopped.

The power supply section 32 supplies power to each section.
While the power is on, the gas supply section 33 drives an internal pump to continue sending gas to the atomized gas chamber 14b of the handpiece 10 through the pipe 20a. In this embodiment, the surrounding air continues to be fed as a gas, but a gas cylinder (not shown) is installed to supply an appropriate gas such as oxygen, hydrogen, or an appropriate mixed gas. be able to.

FIG. 3 is a flowchart showing the operation of the multi-component skin penetration system 1.
When the operation start button 30a (see FIG. 1) is pressed to turn on the power, the control unit 19d accepts input of the first spraying liquid from the liquid input hole 15b (step S1). The received first spray liquid is supplied from the liquid storage section 15a to the spray liquid chamber 13.

The control unit 19d accepts switching of the spray setting by the switching button 19b (step S2).

When the actuation button 19c is pressed, the control unit 19d performs a first liquid spraying step of performing spraying according to the spray settings (step S3). That is, if the spray setting is manual spraying, spraying is executed at the timing when the manual spray button 16 is pressed, and if the spray setting is first intermittent spraying, intermittent spraying is automatically performed at the first time interval. If the spray setting is the second intermittent spraying, the intermittent spraying at the second time interval will be automatically continued, and if the spray setting is the third intermittent spraying, the intermittent spraying will be performed at the third time interval. to continue running automatically.

When the spray settings are changed (step S4: YES), the control unit 19d accepts the change in the spray settings (step S2), and executes spraying with the spray settings (step S3).

When spraying this spraying liquid (first spraying liquid, second spraying liquid, etc.), the tip of the spray cover 11 is brought into contact with the skin. When the circular ring-shaped tip of the spray cover 11 is completely brought into contact with the skin and spraying is performed, excess gas is released from the gas vent space 11a at the tip along with the spray liquid. Therefore, most of the spray liquid in the form of a fine mist penetrates into the skin due to the force of release, and excess gas is released from the gas removal space 11a to press the skin with the pressure of the gas. You can prevent this from happening too much.

That is, if spraying is performed with the entire circumference of the tip of the spray cover 11 in contact with the skin without the gas vent space 11a, the skin will be pressed by the released gas and the spray liquid, and this Since the amount of gas in the spray cover 11 has increased, the skin remains pressed and depressed. This puts extra pressure on your skin, which can actually slow blood flow and prevent the spray liquid from penetrating your skin. On the other hand, by releasing excess gas from the gas removal space 11a, there is no continued pressure on the skin, and the skin returns to its normal state immediately after the gas and spray liquid are sprayed onto the skin. return. For this reason, the spray liquid that has penetrated into the skin can be absorbed into the bloodstream and lymph flow without stress, and the spray liquid that has penetrated the skin is given a mild and moderate stimulation, promoting blood flow and lymph flow. will go deeper.

If the spraying of the first spraying liquid is completed but not completed (step S5: NO), a second spraying liquid spraying step of accepting the second spraying liquid is performed (step S6). The received second spray liquid is supplied to the spray liquid chamber 13 from the liquid storage section 15a. After this, the processes from step S2 onwards are repeated, and the second spraying liquid is sprayed.

With the above configuration and operation, the active ingredient can be sprayed in the form of fine mist. Since only a small amount of spray liquid is used per spray (instantaneous spray, e.g. 0.1 seconds), a large number of sprays can be obtained in small quantities, even with valuable and expensive active ingredients. Therefore, it is possible to spray the entire face one by one, and it is also possible to spray the neck and décolleté. It can also be sprayed onto the head, such as spraying the entire head or spraying multiple times to areas of the head where it is desired to be concentratedly penetrated.

If human stem cell culture solution is used as the penetrant (first penetrant or second penetrant), spraying it on the skin of the face, neck, décolletage, etc. will increase the firmness, have a lifting effect, and open the eyes. The effects include reducing wrinkles, slimming contours, and making the skin look fresh and rejuvenated. In particular, these effects are more pronounced when human umbilical cord stem cell culture supernatant is used.

In addition, when spraying human stem cell culture solution on injured or burnt areas of the skin, the effect is that the wounds or burns heal faster than normal, and even after they heal, there is no scar. You can achieve the effect of leaving it in a clean state without any residue. In particular, these effects are more pronounced when human umbilical cord stem cell culture supernatant is used.

In addition, when a human stem cell culture solution is used as the penetrant (first penetrant or second penetrant) and sprayed on the head, the hair growth effect and hair volume increase. Effect, hair growth effect such as advancing the hairline, increasing the firmness of the hair, increasing the elasticity of the hair, increasing the thickness of the hair, the effect of turning gray hair back to black, and the effect of making you look younger. You can get it. In particular, these effects are more pronounced when human umbilical cord stem cell culture supernatant is used.

In addition, if NMN is used as a penetrant (first penetrant or second penetrant), spraying it on the skin of the face, neck, décolletage, etc. will increase the firmness, lift up effect, and open the eyes. , the effect of reducing wrinkles, narrowing the contours, and making the skin look fresh and rejuvenated.

In addition, when NMN is sprayed on injured or burned areas of the skin, the effect is that the wounds or burns heal faster than normal, and even after healing, no marks remain. The effect of creating a beautiful state can be obtained.

In addition, when NMN is used as a penetrant (first penetrant or second penetrant) and is sprayed on the head, it has the effect of growing hair, increasing hair volume, and improving the hairline. You can get the effect of making the hair move forward, the effect of increasing the elasticity of the hair, the effect of making the hair stronger, the effect of making the hair thicker, the effect of turning gray hair back to black hair, etc.

Furthermore, when human stem cell culture medium is used as one of the first penetrant and the second penetrant and NMN is used as the other, each of the above-mentioned effects can be obtained more markedly than when using either one of them. It is possible to achieve even greater effects by combining both than by using twice the amount of one.

In particular, when spraying the first spraying liquid using the first penetrant and the second spraying liquid using the second penetrating agent are performed separately and in order, these active ingredients are added one by one. They can be penetrated into the skin, and each penetrates sequentially through blood and lymph flow, so they can have a synergistic effect. Particularly when this type of treatment is performed on the face, remarkable effects can be obtained in changes in the outline and size of the eyes, and the results are so great that some people suspect that they have undergone cosmetic surgery. Obtainable.

Moreover, since the first spraying liquid and the second spraying liquid are sprayed by the spraying device 5, each spray can be performed in a constant amount and with a constant intensity. Therefore, regardless of the skill of the practitioner, the same effect can be obtained no matter who performs the treatment as long as the spray is applied to the same area in the same order.

An example of a multi-component skin permeation method using the multi-component skin permeation system 1 described above will be described below.
The first penetrant 2, second penetrant 3, and conditioning agent 4 used in this example are as follows.

First penetrant 2: NMN
<Details>
・β-nicotinamide mononucleotide ・Particle size: 16 mesh or less ・Bulk specific gravity: 0.6 g/ml or more ・Purity 99% or more (component ratio: 100%)
・Processing: Micro granulation processing ・Shape: White crystal powder ・Amount: 500mg
・Amount used in this example: 1/3 (=166.67mg)

Second penetrant 3: Human umbilical cord stem cell culture supernatant <Details>
・Umbilical cord matrix-derived human mesenchymal stem cells (hMSC-UC)
・Proliferation cell culture ・Passage 2 (3rd culture)
・Storage method: Cryopreservation ・Amount: 1ml
・Amount of materials in this example: Total amount

Conditioner 4: Purified water/tap water 400,000 times more pure/Amount: 400ml
・Amount used in this example:
(1) Use 3 ml to mix with 166.67 g of NMN powder as the first penetrant 2... After mixing, about 3.17 ml of second spray liquid
(2) Use 2 ml to mix with 1 ml of human umbilical cord stem cell culture supernatant liquid as the second penetrant 3...After mixing, 3 ml of the first spray liquid

0. Preparation First, the subject's skin was cleansed and a massage was performed to drain the lymph from the neck, décolletage, and face as a preliminary preparation.

1. Spraying of the first spraying liquid (NMN)
Using the spray device 5, the first spray liquid was sprayed onto the décolleté, neck, and face. The spraying was performed one by one in sequence with the tip of the spray cover 11 in contact with the skin. The number of times of spraying was approximately 1,000 times in total.

2. Second spray liquid spray (human umbilical cord stem cell culture supernatant)
Using the spray device 5, the second spray liquid was sprayed onto the décolleté, neck, and face. The spraying was performed one by one in sequence with the tip of the spray cover 11 in contact with the skin. The number of times of spraying was approximately 1,000 times in total.

As a result of performing the above treatment on two people, the following effects were obtained.

In particular, since the change in the contour is easy to understand, an example of the change in subject 1 will be explained with reference to FIG. 4. FIG. 4(A) is a photograph at the stage where the above-mentioned "0. Preparation" has been completed, that is, the stage where the massage for draining the lymph has been completed.
FIG. 4(B) is a photograph of the state after spraying NMN and further spraying human umbilical cord stem cell culture supernatant.
FIG. 4(C) is a photograph showing a superimposed state of the facial contour of FIG. 4(A) before spraying and the facial contour of FIG. 4(B) after spraying.
In this way, it is clearly seen that a great effect is obtained by performing this treatment, in which NMN and human stem cell culture solution are applied sequentially, only once (after) from the state after the lymph has been drained (before).

In addition, the next day, the subject was told by a friend that he might have gone to get plastic surgery, and a person he met a week after the procedure also told him that he might have gotten plastic surgery. In other words, it can be seen that a great effect can be obtained just by performing this treatment in which NMN and human stem cell culture solution are sequentially applied once, and that the effect is sustained.

In Example 2, only the order of spraying was changed from Example 1. That is, the first penetrant 2 and the second penetrant 3 were replaced as follows.
(1) Use 2 ml to mix with 1 ml of human umbilical cord stem cell culture supernatant liquid as the first penetrant 2...After mixing, 3 ml of the first spray liquid
(2) Use 3 ml to mix with 166.67 g of NMN powder as the second penetrant 3...After mixing, about 3.17 ml of second spray liquid
Other than this, the contents were the same as in Example 1, and massage, spraying of the first spraying liquid, and spraying of the second spraying liquid were performed in this order.
As a result of performing the above treatment on two people, the following effects were obtained.

Furthermore, since Subject 3 had atopic dermatitis, the first spraying liquid (human umbilical cord stem cell culture supernatant liquid) and the second spraying liquid (NMN) were similarly sprayed onto the atopic area. As a result, the symptoms of atopic dermatitis were improved, and the skin in the atopic area was improved in moisture, firmness, and luster.

Next, using the same prescription and treatment as in Example 1, the product was sprayed onto the scalp to confirm the hair growth effect. At this time, in the preparatory massage, the back of the neck and scalp were massaged. Other than that, the spraying liquid and the order of treatment were the same as in Example 1.
A series of treatments was treated as one treatment, and the treatment was performed a total of four times to measure the effects. The interval between each session ranged from 1 week to 1 month.
As a result of the treatment performed on two people, clear hair growth effects were seen in both patients.

Next, using the same prescription and treatment as in Example 2, the product was sprayed onto the scalp to confirm the hair growth effect. At this time, in the preparatory massage, the back of the neck and scalp were massaged. The other spraying liquid and treatment order were the same as in Example 2.
A series of treatments was treated as one treatment, and the treatment was performed a total of four times to measure the effects. The interval between each session ranged from 1 week to 1 month.
As a result of the treatment performed on two people, clear hair growth effects were seen in both patients.

This invention is not limited to the present embodiment described above, and can be made into various other embodiments.

For example, before the first spraying liquid is sprayed, holes are made on the skin surface using a roller having a plurality of fine needles of a predetermined length arranged around the outer periphery of the cylinder, and then the first spraying liquid is sprayed, and the second spraying liquid is sprayed. It may also be followed by spraying an atomizing liquid. In this case, the permeation of the spray liquid into the skin can be improved, and more effects can be obtained. In this case, the needle is preferably made of titanium. Further, the predetermined length can be 0.1 mm to 1 mm, preferably 0.1 to 0.5 mm, and preferably 0.25 mm.

Also, if you perform a massage to drain the lymph as a preliminary preparation, you can get better effects (effects from the massage + effects from the spray) because the flow of blood and lymph will improve, but if you do not perform this kind of preparation in advance, you can get better effects. The first spraying liquid may be sprayed and the second spraying liquid may be sprayed. Even in this case, a clear change can be obtained between before and after spraying.

In addition, although the first spraying liquid was sprayed 1,000 times and the second spraying liquid was sprayed 1,000 times, the invention is not limited to this, and the spraying may be 500 times each, or 300 times each. Alternatively, the number of times can be set as appropriate, such as increasing the number of times to 1,500 times. The number of times of spraying depends on the area of the skin to be sprayed, but for one type of spray liquid, it can be 10 times or more, preferably 50 times or more, and 100 times or more. More preferably, the number of times is 300 or more, and more preferably 500 or more times. Further, the upper limit of the number of times of spraying can be 3000 times or less for one type of spraying liquid, preferably 2000 times or less, more preferably 1500 times or less, and 1000 times or less. It is preferable that

With respect to the area of the skin to be applied, the range covered inside the tip of the circular spray cover 11 can be applied to 40% or more, preferably 50% or more, and 60% or more. It is more preferable to apply it, more preferably to apply it 70% or more, even more preferably to apply it 80% or more, and preferably to apply it 90% or more. Further, although spraying to the same location may be done once, more of the active ingredient may be penetrated by spraying two or more times. In particular, it is preferable to spray twice or more locally on areas that require intensive care, such as fine lines around the eyes, nasolabial folds, and wrinkles on the forehead. This increases the penetration of the active ingredient into the area that you want to intensively care for, making it possible to obtain more favorable effects on the area that you want to intensively care for.

In addition, regarding the spray liquid using the first penetrant 2 and the second penetrant 3, it is possible to simultaneously charge all the components by mixing both of them and the conditioning agent 4 and then putting them into the liquid storage section 15, or to add the first penetrant 2 and the second penetrant 2 and the conditioning agent 4 are mixed and put into the liquid storage part 15 as a first spraying liquid to be sprayed, and then within a predetermined time, the second penetrant 3 and conditioning agent 4 are mixed and used as a second spraying liquid. Any method of sequentially charging each component into the liquid storage section 15 can be used.

The predetermined time from the completion of spraying of the first spraying liquid to the start of spraying of the second spraying liquid in sequential injection by component can be within one week, preferably within 48 hours, and preferably within 24 hours. It is preferably within 12 hours, more preferably within 6 hours, even more preferably within 3 hours, even more preferably within 1 hour, and even more preferably within 30 minutes. More preferably, the heating time is preferably within 10 minutes. By adding them sequentially in this way, the components of the first penetrant 2 and the components of the second penetrant 3 are sufficiently penetrated, and the effects of both components are obtained at the same time, creating a synergistic effect. It can be effective. In particular, by shortening the predetermined time from the spraying of the first spraying liquid (first penetrant 2 + conditioning agent 4) to the spraying of the second spraying liquid (second penetrating agent 3 + conditioning agent 4), synergistic effects of both components can be achieved. The effect can be increased. Also, by adding the ingredients one after another, rather than mixing them all and adding them at the same time, each ingredient can be thoroughly absorbed.

When all components are added at the same time, the overall spraying time can be shortened compared to when each component is added sequentially. For example, if the ingredients are added sequentially, the first spraying liquid is sprayed on each part of the face (you may also add the neck and décolletage) 1000 times, and then the second spraying liquid is sprayed on the face again. It is necessary to spray 1,000 times on each part of the face (the neck and décolletage may be added), which takes time for a total of 2,000 sprays. , décolletage may be added) can be sprayed a total of 1000 times, cutting the time in half.

In the case of adding all the ingredients at the same time, in addition to the first penetrant 2 and the second penetrant 3, another third penetrant (an agent composed of the third active ingredient) is further mixed, and then all the ingredients are added at the same time. It can also be poured and sprayed. The third penetrating agent can be any suitable substance, for example a cannabinoid (third active ingredient) such as CBD (cannabidiol), CBN (cannabinol) or CBG (cannabigenol), When using this cannabinoid, it is preferable that THC (tetrahydrocannabinol) be removed.

The third penetrant can also be a proteoglycan. In this case, by applying it especially to the head, it can have effects on the hair (hair growth effect, hair volume increase, hairline moving forward, hair firmness increasing effect, etc.) The effect of increasing the elasticity of the hair, the effect of increasing the thickness of the hair, the effect of turning gray hair back to black, etc.) can be improved.

In the case of sequential injection by component, after spraying the first spraying liquid (first penetrant 2 + regulator 4) and the second spraying liquid (second penetrant 3 + regulator 4), add the third spraying liquid ( It is also possible to spray the third penetrant + conditioning agent 4) within a predetermined time. Furthermore, it is also possible to add the third spraying liquid (fourth penetrant + conditioning agent 4) within a predetermined period of time.

In addition, the first active ingredient was NMN and the second active ingredient was a human stem cell culture solution, which was applied (sprayed) in this order, but the order was reversed and the first active ingredient was a human stem cell culture solution, and the second active ingredient was used as a human stem cell culture solution. NMN may be applied (sprayed) in this order. In this case as well, each active ingredient can be infiltrated in sequence to exhibit their respective synergistic effects.

Alternatively, one of the first active ingredient and the second active ingredient may be applied (sprayed) as a human stem cell culture solution and the other as a proteoglycan, respectively. In this case as well, each effect can be fully exerted and a synergistic effect can be obtained.

Further, the method of application is preferably the above-mentioned spraying, but is not limited to this, and other methods may be used, such as applying it to the skin by hand, or allowing it to penetrate into a pack or the like and placing it on the skin. By making multiple small holes in the skin using a fine needle, the penetration of the active ingredients can be increased. In this case as well, a certain degree of effect can be obtained, and by applying them in order, the synergistic effect of each active ingredient can be exerted.

This invention can be used in industries that provide beauty salons and hair growth salons.

1...Multi-component skin penetration system 12a...Spray port 15a...Liquid storage section 17...Solenoid 19d...Control section 33...Gas supply section

Claims (5)

Applying a first composition containing either a first active ingredient in liquid or powder form consisting of a human stem cell culture solution and a second active ingredient in liquid or powder form consisting of NMN;
applying a second composition containing the other of the first active ingredient and the second active ingredient ,
The first composition does not contain the other active ingredient contained in the second composition,
The second composition does not contain the one active ingredient contained in the first composition.
Skin beauty methods (excluding medical procedures). In the step of applying the first composition and the step of applying the second composition, the one active ingredient and the other active ingredient are not added simultaneously, but the one active ingredient and the other active ingredient are added together. This is done by sequentially adding active ingredients.
The skin beauty method according to claim 1 (excluding medical treatment). The step of applying the first composition is a step of spraying a spray liquid prepared by adjusting one of the active ingredients with purified water or physiological saline,
The skin beauty method according to claim 2, wherein the step of applying the second composition is a step of spraying a spray liquid prepared by adjusting the other active ingredient with purified water or physiological saline. , excluding medical procedures). The atomization is performed by releasing compressed gas at high speed in a short time of 0.001 seconds or more and 1 second or less, thereby nano-izing the atomizing liquid and releasing it.
Skin beauty method according to claim 3 (excluding medical treatment). The step of applying the first composition and the step of applying the second composition are performed within 48 hours.
Skin beauty method according to claim 4 (excluding medical treatment).

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