JP7365208B2 - beverage composition - Google Patents

Description

BEVERAGE COMPOSITIONS FIELD OF THE INVENTION The present invention relates to beverage compositions.

Ornithine is a type of amino acid and has various health functions, so its application to foods and drinks is being considered. For example, adding 0.5 to 15% lemon juice to a solution containing 0.08% of L-ornithine salt as free ornithine and adjusting the pH to 3.5 to 3.6 can suppress microbial growth in beverages. BACKGROUND ART A packaged beverage that is compatible with maintaining flavor balance and has health functions and palatability has been proposed (Patent Document 1).

On the other hand, cineole is a type of monoterpenoid having a cyclic ether structure, and is known as an aromatic substance having a refreshing scent. Conventionally, it has been reported that when cineole is added to malt beverages, it is possible to suppress the deterioration of taste due to light (Patent Document 2).

Japanese Patent Application Publication No. 2011-120479 Japanese Unexamined Patent Publication No. 59-179058

When the present inventors added ornithine to a low-acidity beverage, the sour taste was strongly suppressed due to the strong taste peculiar to ornithine. Therefore, when a large amount of acidulant was added to enhance the sour taste, a new problem arose in that the high concentration of acidulant caused irritation to the throat.
An object of the present invention is to provide a beverage composition that is enriched with ornithine while reducing the irritation to the throat caused by a high concentration of acidulant.

The present inventors have discovered that when a specific monoterpenoid is contained, a beverage composition can be obtained that strengthens ornithine while reducing the irritation to the throat caused by a high concentration of acidulant.

The present invention comprises the following components (A), (B) and (C);
(A) at least one selected from ornithine and its salts 0.1 to 1.7% by mass,
(B) cineole 0.01 to 1000 ppb by mass, and (C) containing an acidulant,
(D) A beverage composition having an acidity of more than 0.5% by mass and 5.0% by mass or less is provided.

According to the present invention, it is possible to provide a beverage composition in which the irritation to the throat caused by the acidulant is alleviated while enhancing ornithine.

The beverage composition of the present invention contains at least one selected from ornithine and its salt as component (A).
Component (A) may be in the L form, the D form, or a mixture thereof (eg, racemic form), but the L form is preferable. Component (A) may be a naturally derived product, a chemically synthesized product, or a commercially available product. Naturally derived products include, for example, extracts from freshwater clams, and chemically synthesized products include, for example, those produced by fermentation methods, and may be purified by column chromatography or the like if necessary.

Examples of ornithine salts include acid addition salts, amino acid addition salts, organic amine addition salts, ammonium salts, metal salts, and the like. Examples of acid addition salts include inorganic acid salts such as hydrochloride, sulfate, nitrate, phosphate, acetate, maleate, fumarate, citrate, malate, lactate, α-ketoglutarate, etc. Examples include organic acid salts such as acid salts, gluconates, and caprylates. Examples of amino acid salts include glycine salts, phenylalanine salts, lysine salts, aspartates, and glutamates, and examples of organic amine addition salts include morpholine salts and piperidine salts. Examples of ammonium salts include ammonium salts and tetramethylammonium salts; examples of metal salts include alkali metal salts such as sodium salts and potassium salts; alkaline earth metal salts such as magnesium salts and calcium salts; Examples include aluminum salts and zinc salts. Two or more ornithine salts may be used in combination.

Among these, as component (A), ornithine salts are preferred, ornithine acid addition salts and ornithine amino acid salts are more preferred, and L-ornithine hydrochloride and L-ornithine L-aspartate are even more preferred.

The content of component (A) in the beverage composition of the present invention is 0.1 to 1.7% by mass, but from the viewpoint of strengthening ornithine and physiological effects, it is preferably 0.2% by mass or more, and 0.1% to 1.7% by mass. .3 mass% or more is more preferable, 0.4 mass% or more is still more preferable, and from the viewpoint of strongly suppressing the taste derived from ornithine, 1.5 mass% or less is preferable, and 1.4 mass% or less is more preferable. It is more preferably 1.3% by mass or less, particularly preferably 1.0% by mass or less. The content range of component (A) in the beverage composition is preferably 0.2 to 1.5% by mass, more preferably 0.3 to 1.4% by mass, and even more preferably is from 0.4 to 1.3% by weight, particularly preferably from 0.4 to 1.0% by weight. In addition, when component (A) is in the form of a salt, the content of component (A) is a value converted to free ornithine. Moreover, the content of component (A) can be measured by an analytical method suitable for the situation of the measurement sample among commonly known measuring methods, and for example, an amino acid analyzer can be used. Specifically, the method described in Examples below may be mentioned. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.

The beverage composition of the present invention contains cineole as component (B).
"Cineole" according to the present specification is also referred to as 1,8-cineole, and the IUPAC system name is 1,3,3-trimethyl-2-oxabicyclo[2.2.2]octane. Component (B) may be derived from raw materials or may be newly added. In addition, the origin of component (B) is not particularly limited as long as it is commonly used in the field of food and beverages, and for example, it may be a chemically synthesized product, or a cineole-containing plant extract or essential oil.

The content of component (B) in the beverage composition of the present invention is 0.01 to 1000 ppb by mass, but from the viewpoint of alleviating the irritation to the throat caused by the high concentration of acidulant, the content of component (B) is 0.03 ppb or more by mass. It is preferably 0.05 mass ppb or more, more preferably 0.1 mass ppb or more, even more preferably 0.3 mass ppb or more, particularly preferably 1.0 mass ppb or more, and from the viewpoint of flavor balance. , is preferably 500 mass ppb or less, more preferably 300 mass ppb or less, even more preferably 100 mass ppb or less, even more preferably 70 mass ppb or less, and particularly preferably 40 mass ppb or less. The content range of component (B) in the beverage composition of the present invention is preferably 0.03 to 500 mass ppb, more preferably 0.05 to 300 mass ppb, and more preferably 0. .1 to 100 mass ppb, more preferably 0.3 to 70 mass ppb, still more preferably 1.0 to 40 mass ppb. In addition, the content of component (B) can be measured by an analysis method suitable for the situation of the measurement sample among commonly known measurement methods, for example, it can be analyzed by the GC/MS method. It is. Specifically, the method described in Examples below may be mentioned. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.

The beverage composition of the present invention contains an acidulant as component (C).
Component (C) may be an organic acid, an inorganic acid, or a salt thereof, and is not particularly limited as long as it is used in foods and drinks. Component (C) may be contained alone or in combination of two or more.
Examples of organic acids include citric acid, gluconic acid, malic acid, tartaric acid, ascorbic acid, succinic acid, lactic acid, fumaric acid, adipic acid, phytic acid, and fumaric acid. Further, examples of the inorganic acid include phosphoric acid. Examples of the salt include alkali metal salts such as potassium and sodium. Among these, from the viewpoint of sour taste quality, one or more selected from citric acid, malic acid, tartaric acid, and salts thereof are preferred, and at least one selected from citric acid and salts thereof is more preferred. As the salt, alkali metal salts such as potassium and sodium are preferred.

The content of component (C) in the beverage composition of the present invention can be appropriately determined depending on the type of acidulant so as to achieve the acidity described below.
The acidity (D) of the beverage composition of the present invention is more than 0.5% by mass and not more than 5.0% by mass, but from the viewpoint of suppressing the strong taste peculiar to ornithine, it is preferably 0.6% by mass or more, and 0.6% by mass or more. It is more preferably 7% by mass or more, and from the viewpoint of sourness strength, it is preferably 3.0% by mass or less, more preferably 2.5% by mass or less, and preferably 2.0% by mass or less. The range of such acidity is preferably more than 0.5% by mass and 3.0% by mass, more preferably 0.6 to 2.5% by mass, and still more preferably 0.7 to 2.0% by mass. It is. Here, in this specification, "acidity" refers to the concentration of all acids contained in the beverage composition, which is determined by titrating the beverage composition with sodium hydroxide using a phenolphthalein indicator. It is converted as a quantity. Specifically, it can be measured by the method described in Examples below.

The beverage composition of the present invention can contain non-polymerized catechin as component (E).
Here, in this specification, "non-polymer catechins" refer to non-epi catechins such as catechin, gallocatechin, catechin gallate, and gallocatechin gallate, as well as epicatechin, epigallocatechin, epicatechin gallate, and epigallocatechin. It is a general term for epi-catechins such as gallate, and in the present invention, it is sufficient to contain at least one of the above eight types. Note that the content of non-polymer catechins is defined based on the total amount of the above eight types.

The content of component (E) in the beverage composition of the present invention is 0.01 to 0.5% by mass, but from the viewpoint of physiological effects, it is preferably 0.015% by mass or more, and 0.02% by mass or more. is more preferable, and from the viewpoint of flavor balance, it is preferably 0.4% by mass or less, more preferably 0.3% by mass or less, and even more preferably 0.2% by mass or less. The content range of component (E) in the beverage composition of the present invention is preferably 0.01 to 0.4% by mass, more preferably 0.015 to 0.3% by mass. , more preferably 0.02 to 0.2% by mass. Note that the content of component (E) in the beverage composition can be analyzed by an analytical method suitable for the situation of the measurement sample among commonly known analytical methods, such as liquid chromatography. Specifically, it can be analyzed by the method described in Examples below. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.

The beverage composition of the present invention can contain chlorogenic acids as component (F).
In this specification, "chlorogenic acids" refer to monocaffeoylquinic acids such as 3-caffeoylquinic acid, 4-caffeoylquinic acid, and 5-caffeoylquinic acid, and 3-ferulaquinic acid and 4-ferulaquinic acid. Acid and monoferulaquinic acid of 5-ferulaquinic acid and dicaffeoylquinic acids of 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid are combined. In the present invention, at least one of the above nine types may be contained. Note that the content of chlorogenic acids is defined based on the total amount of the above nine types.

The content of (F) in the beverage composition of the present invention is 0.005 to 0.5% by mass, but from the viewpoint of physiological effects, it is preferably 0.008% by mass or more, and more preferably 0.01% by mass or more. Preferably, from the viewpoint of flavor balance, the content is preferably 0.4% by mass or less, more preferably 0.3% by mass or less, and even more preferably 0.2% by mass or less. The content range of component (F) in the beverage composition of the present invention is preferably 0.005 to 0.4% by mass, preferably 0.008 to 0.3% by mass, More preferably, it is 0.01 to 0.2% by mass. Note that the content of component (F) in the beverage composition can be analyzed by an analytical method suitable for the situation of the measurement sample among commonly known analytical methods, such as liquid chromatography. Specifically, it can be analyzed by the method described in Examples below. In addition, during measurement, appropriate processing is performed as necessary, such as freeze-drying the sample to match the detection range of the device, or removing impurities from the sample to match the separation capability of the device. It may be applied.

The beverage composition of the present invention may also contain sweeteners, vitamins, minerals, antioxidants, foam stabilizers, esters, pigments, emulsifiers, preservatives, seasonings, fruit juices, vegetable juices, nectar extracts, vegetable oils and fats, if desired. The composition may contain one or more additives such as quality stabilizers and the like. The content of the additive can be appropriately selected within a range that does not impair the purpose of the present invention.

The beverage composition of the present invention may be in any suitable form, for example liquid or solid.
For example, when the beverage composition of the present invention is liquid, the beverage form may be an RTD (ready to drink) or a concentrated beverage. Among these, RTD is preferred from the viewpoint of convenience. Here, in this specification, "RTD" refers to a beverage that can be drunk as is without dilution.

When the beverage composition of the present invention is solid, its shape is not particularly limited as long as it is solid at room temperature (20°C ± 15°C), such as powder, granule, tablet, rod, plate, block, etc. It can be of various shapes. The solid content in the solid beverage composition of the present invention is usually 95% by mass or more, preferably 97% by mass or more. Note that the upper limit of the solid content is not particularly limited, and may be 100% by mass. Here, in this specification, "solid content" refers to the mass of the residue after drying the sample in an electric constant temperature dryer at 105° C. for 3 hours and removing volatile substances.

When the beverage composition of the present invention is a concentrate or solid, when the beverage is prepared by diluting with liquid according to the prescribed usage, the content and acidity of components (A) and (B) meet the above requirements. All you have to do is satisfy. The liquid is not particularly limited as long as it can be reduced to a drink, and examples thereof include water, carbonated water, milk, soy milk, etc., and the temperature of the liquid does not matter. Note that the dilution ratio may be determined according to a prescribed usage method, but in the case of a solid beverage composition, it is usually 20 to 600 times by mass, preferably 30 to 500 times by mass, more preferably 40 to 250 times by mass, and even more preferably The amount is 50 to 200 times by weight, particularly preferably 50 to 150 times by weight. In addition, in the case of a concentrated and reconstituted beverage composition, the dilution ratio is usually 1.5 to 200 times by mass, preferably 1.5 to 100 times by mass, more preferably 1.8 to 50 times by mass, even more preferably 2 to 200 times by mass. It is 30 times the mass.

In addition, when the beverage composition of the present invention is RTD, it may be used in ordinary packaging such as molded containers containing polyethylene terephthalate as a main component (so-called PET bottles), metal cans, paper containers composited with metal foil or plastic film, bottles, etc. It can be provided in containers.
Furthermore, when the beverage composition of the present invention is RTD, it may be heat sterilized. The heat sterilization method is not particularly limited as long as it complies with the conditions stipulated by applicable laws and regulations (in Japan, the Food Sanitation Law). Examples include a retort sterilization method, a high temperature short time sterilization method (HTST method), an ultra high temperature sterilization method (UHT method), and a post-filling sterilization method (pastorization). In addition, it is also possible to select an appropriate heat sterilization method depending on the type of container. For example, if the container can be heat sterilized after filling the container with beverages, such as metal cans and bottles, retort sterilization is recommended. or post-filling sterilization method (pastorization). In addition, for items that cannot be sterilized by retort, such as PET bottles, we will adopt aseptic filling, where the beverage is heat sterilized under the same sterilization conditions as above, and then filled into sterilized containers in a sterile environment, or hot pack filling. be able to.

The beverage composition of the present invention can be manufactured by an appropriate method, but for example, component (A), component (B), component (C), and other components may be blended as necessary to adjust the acidity. It can be manufactured using

1. Analysis of ornithine and its salts After accurately weighing 0.5 g of the sample, add 25 mL of 10 w/v% sulfosalicylic acid solution, and then add 3 mol/L sodium hydroxide solution and mix. After that, the pH was adjusted to 2.2 with sodium citrate buffer and the volume was adjusted to 100 mL. After filtration with a membrane filter (GL Chromato Disc 13A, pore size 0.2 μm, GL Sciences, Inc.), amino acid automatic analysis was performed. Serve.

Amino acid automatic analyzer operating conditions/model: JLC-500/V (JEOL Ltd.)
・Column: LCR-6, φ4mm x 120mm (JEOL Co., Ltd.)
・Mobile phase: Lithium citrate buffer (P-12 to P-15, P-21) (JEOL Ltd.)
・Reaction solution: Ninhydrin coloring solution kit for JEOL Ltd.-II (Wako Pure Chemical Industries, Ltd.)
・Flow rate: Mobile phase 0.50mL/min, reaction liquid 0.30mL/min
・Measurement wavelength: 570nm

2. Analysis of Cineol A 10 mL sample was collected into a GC headspace vial (20 mL), and 3 g of sodium chloride was added. A stirrer was placed in the vial, the vial was sealed tightly, and the components were adsorbed onto SPME fiber (manufactured by Sigma-Aldrich, 50/30 μm, DVB/CAR/PDMS) while stirring with a stirrer for 30 minutes. After adsorption, the SPME fiber was heated and desorbed at the injection port, and GC/MS measurement was performed. The analytical instrument used was Agilent 6890N/5975C (manufactured by Agilent Technologies).

The analysis conditions are as follows.
・Column: VF-WAX (length 60m, inner diameter 0.25mm, film thickness 0.25μm)
・Column temperature: 35℃ (4min) → 3℃/min → 130℃ → 5℃/min → 240℃ (15min)
・Column pressure: Constant flow mode (31kPa)
・Column flow rate: l. 5mL/min (He)
・Inlet temperature: 240℃
・Injection method: Splitless ・Detector: MS
・Ion source temperature: 240℃
・Ionization method: EI (70eV)

A standard sample was prepared by dissolving the purchased reagent in ethanol and serially diluting it. A sample at a predetermined concentration was added to the sample, adsorbed onto the SPME fiber in the same manner as the sample alone, and GC/MS measurement was performed. In addition, the peak area of ion of m/z 154 was used for quantitative determination of cineole.

3. Analysis of acidity 1) Titration Accurately weigh 5 to 15 g of the sample into a 200 mL Erlenmeyer flask, dilute it appropriately with water, add a few drops of 1% phenolphthalein indicator, and add 0.1 M sodium hydroxide in a 25 mL burette. Titrate while shaking. The end point is the point where the red color persists for 30 seconds. When using a hydrogen ion concentration meter, titrate in the same manner while stirring with a magnetic stirrer, and the end point is when the pH reaches 8.1.

2) Calculation Calculate the acidity using the following formula (1).

Acidity (mass%) = A x f x 100/W x 0.0064 (1)
[In formula (1), A represents the titration amount (mL) with a 0.1M sodium hydroxide solution, f represents the titer of the 0.1M sodium hydroxide solution, and W represents the sample mass (g), respectively. ]

4. Analysis of non-polymer catechins A sample dissolved and diluted with pure water was analyzed using a high-performance liquid chromatograph (model SCL-10AVP, manufactured by Shimadzu Corporation) using an octadecyl group-introduced packed column for liquid chromatography (L-column ODS, 4. 6 mmφ x 250 mm, particle size 5 μm (manufactured by the Japan Chemical Evaluation and Research Institute) was installed, and the column temperature was 35° C. and measured by the gradient method. Mobile phase A solution was a distilled aqueous solution containing 0.1 mol/L of acetic acid, B solution was an acetonitrile solution containing 0.1 mol/L of acetic acid, the flow rate was 1 mL/min, the sample injection amount was 10 μL, and the UV detector wavelength was 280n
Perform under the conditions of m. Note that the gradient conditions are as follows.

Concentration gradient conditions (volume%)
Time A liquid concentration B liquid concentration 0 minutes 97% 3%
5 minutes 97% 3%
37 minutes 80% 20%
43 minutes 80% 20%
43.5 minutes 0% 100%
48.5 minutes 0% 100%
49 minutes 97% 3%
60 minutes 97% 3%

5. Analysis of chlorogenic acids HPLC was used as the analytical instrument. The model numbers of the component units of the device are as follows.
・UV-VIS detector: L-2420 (Hitachi High Technologies Co., Ltd.),
・Column oven: L-2300 (Hitachi High Technologies, Ltd.),
・Pump: L-2130 (Hitachi High Technologies Co., Ltd.),
・Auto sampler: L-2200 (Hitachi High Technologies, Ltd.),
- Column: Cadenza CD-C18 inner diameter 4.6 mm x length 150 mm, particle diameter 3 μm (Intact Co., Ltd.).

The analysis conditions are as follows.
・Sample injection volume: 10μL,
・Flow rate: 1.0mL/min,
・UV-VIS detector setting wavelength: 325nm,
・Column oven setting temperature: 35℃,
- Eluent C: 0.05M acetic acid, 0.1mM 1-hydroxyethane-1,1-diphosphonic acid, 10mM sodium acetate, 5 (V/V)% acetonitrile solution,
- Eluent D: Acetonitrile.

Concentration gradient conditions (volume%)
Time Eluent C Eluent D
0.0 minutes 100% 0%
10.0 minutes 100% 0%
15.0 minutes 95% 5%
20.0 minutes 95% 5%
22.0 minutes 92% 8%
50.0 minutes 92% 8%
52.0 minutes 10% 90%
60.0 minutes 10% 90%
60.1 minutes 100% 0%
70.0 minutes 100% 0%

In HPLC, 1 g of the sample was accurately weighed, diluted to 10 mL with eluent A, filtered through a membrane filter (GL Chromato Disk 25A, pore size 0.45 μm, manufactured by GL Sciences, Inc.), and then subjected to analysis.

Retention time of chlorogenic acids (1) Chlorogenic acids Monocaffeoylquinic acid: 5.3 minutes, 8.8 minutes, 11.6 minutes total 3 points Monoferulaquinic acid: 13.0 minutes, 19.9 minutes , a total of 3 points of 21.0 minutes ・dicaffeoylquinic acid: a total of 3 points of 36.6 minutes, 37.4 minutes, and 44.2 minutes.
Based on the area values of the nine types of chlorogenic acids determined here, the mass % was determined using 5-caffeoylquinic acid as a standard substance.
(2) Dicaffeoylquinic acids/dicaffeoylquinic acid: Total of 3 points: 36.6 minutes, 37.4 minutes, 44.2 minutes.
From the area values of the three types of dicaffeoylquinic acids determined here, the mass % was determined using 5-caffeoylquinic acid as a standard substance.
Caffeine was analyzed in the same manner as chlorogenic acids, except that the UV-VIS detector was set at a wavelength of 270 nm and caffeine was used as a standard substance. Caffeine retention time is 18.9 minutes.

6. Sensory Evaluation The irritation standard aqueous solution shown in Table 1 was prepared using citric acid, in which the intensity of irritation to the throat was adjusted to four levels. Then, four expert panelists agreed on the evaluation criteria shown in Table 1 for each concentration of the irritation standard aqueous solution, and conducted a sensory test according to the following procedure. First, each expert panel drank standard irritation aqueous solutions in order of decreasing concentration and memorized the intensity of irritation to the throat. Next, each expert panel drinks each drink, evaluates the degree of irritation to the throat, and determines the one with the closest irritation to the throat from among the irritation standard aqueous solutions. Based on the scores determined by each expert panel, final scores were determined in 0.5 increments through discussion.

Examples 1 to 10 and Comparative Examples 1 to 5
A drink was prepared by blending each component shown in Table 2 and performing the same operations as in Example 1. Analysis and sensory evaluation were performed on each of the obtained beverages. The results are also shown in Table 2.

Examples 11 to 13 and Comparative Examples 6 to 8
A drink was prepared by blending each component shown in Table 3 and performing the same operations as in Example 1. Analysis and sensory evaluation were performed on each of the obtained beverages. The results are shown in Table 3 together with the results of Example 4 and Comparative Example 1.

Examples 14 and 15, Comparative Examples 9 and 10 and Reference Examples 1 and 2
Each component shown in Table 4 was blended and a beverage was prepared in the same manner as in Example 1. Analysis and sensory evaluation were performed on each of the obtained beverages. The results are shown in Table 4 together with the results of Example 4 and Comparative Example 1.

Examples 16-21 and Comparative Examples 11-16
A drink was prepared by blending each component shown in Table 5 and performing the same operations as in Example 1. Analysis and sensory evaluation were performed on each of the obtained beverages. The results are shown in Table 5 together with the results of Example 6 and Comparative Example 1.

Example 22, Comparative Example 17 and Reference Example 3
A drink was prepared by blending each component shown in Table 6 and performing the same operations as in Example 1. Analysis and sensory evaluation were performed on each of the obtained beverages. The results are also shown in Table 6.

From Tables 2 to 6, it can be seen that when cineole is contained, a beverage composition can be obtained in which the irritation to the throat caused by a high concentration of acidulant is alleviated while strengthening ornithine.

Claims (5)

The following ingredients (A), (B) and (C);
(A) at least one selected from ornithine and its salts 0.1 to 1.7% by mass,
(B) cineole 0.01 to 1000 ppb by mass, and (C) containing an acidulant,
(D) A beverage composition having an acidity of more than 0.5% by mass and 5.0% by mass or less. The beverage composition according to claim 1, wherein component (C) is one or more selected from citric acid, malic acid, tartaric acid, and salts thereof. The beverage composition according to claim 1 or 2, wherein component (A) is an ornithine acid addition salt or an ornithine amino acid salt. The beverage composition according to any one of claims 1 to 3, further comprising non-polymerized catechins as component (E), and the content of component (E) is 0.01 to 0.5% by mass. thing. The beverage composition according to any one of claims 1 to 4, which further contains chlorogenic acids as component (F), and the content of component (F) is 0.005 to 0.5% by mass.