TWI411399B - Containers of tea drinks - Google Patents

Abstract

Disclosed is a tea beverage packed in a container, which has good flavor and good compositional stability under storage. The beverage is produced by blending a tea extract with another tea extract having a content of a gallate-type catechin in a non-polymeric catechin component of less than 50% by mass. The beverage comprises: (A) 0.072 to 0.4% by mass of a non-polymeric catechin component; and (B) 21 to 150 ppm of gallic acid, wherein the contents of a gallate-type catechin and an epi-type catechin in a non-polymeric catechin component are 0 to 50% by mass and 30 to 60% by mass, respectively.

Description

Container-packed tea drink

The present invention relates to a container-packed tea beverage containing a high concentration of non-polymer catechins and having a good flavor and catechin composition.

As an effect of the catechins, an action of inhibiting the activity of the alpha amylase has been reported (for example, refer to Patent Document 1). In order to express such physiological effects, it is necessary to take a large amount of catechins more easily, and therefore, the manufacturer desires a technique of formulating a high concentration of catechins in a beverage.

As one of the methods, the following method is employed in which a catechin is added to a beverage in a dissolved state by using a tea extract such as a concentrate of green tea extract. However, it has been known that the type of beverage to be used as a target for blending high-concentration catechins, for example, when adding catechins to black tea extracts or carbonated drinks, the bitter taste from caffeine and green tea. Residues are highly detrimental to the commercial value of the beverage.

Since the ancient times, it has been known to carry out tannase treatment on a fermented tea extract such as black tea, and it is possible to suppress the formation of tea cream which is suspended during low-temperature cooling. Further, if the gallic acid catechin is subjected to a tannase treatment as described in Patent Document 1, a part or all of it is made into gallic acid, thereby obtaining catechins and gallic acid. The method of the mixture can reduce the gallate body catechins which are the cause of bitterness. Further, as a method of removing inclusions such as caffeine from the tea extract, adsorption methods (Patent Documents 2 to 4), extraction methods (Patent Document 5), and the like are known.

[Patent Document 1] Japanese Patent Laid-Open Publication No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. No. Hei. Japanese Patent Publication No. 2002-335911 (Patent Document 5) Japanese Patent Laid-Open No. Hei 1-288847

The present invention provides a container-packed tea beverage which is prepared by blending a tea extract with a non-polymer catechin having a gallate catechin content of less than 50% by mass in a tea extract, which comprises : (A) 0.072 to 0.4% by mass of non-polymer catechins, and (B) 21 to 150 ppm of gallic acid, and non-polymer catechins of gallate body catechu The prime rate is 0 to 50% by mass, and the epic body rate is 30 to 60% by mass.

In the mixture of catechins and gallic acid obtained by the tannase treatment, there is a problem of producing a sour taste or a pungent taste. On the other hand, it has been found that those who do not perform the tannase treatment or who are not obtained when the tannase treatment is insufficient are caused by changes in the composition of the catechins as an active ingredient, or changes in color tone and flavor. The problem of loading beverages in containers.

Accordingly, the present invention provides a container-packed tea beverage which not only lowers the rate of gallate body catechins, but also has a high concentration of non-polymer catechins, and suppresses bitterness, and is inhibited even if stored for a long period of time. The composition of the catechins of the active ingredient is changed.

Therefore, the present inventors have found that when a container-packed tea beverage is prepared by using a tea extract in which the gallate body catechin rate is adjusted to less than 50% by mass in advance, the content of gallic acid is adjusted to 21 ~150 ppm, the rate of gallate body catechin in non-polymer catechins is adjusted to 0-50% by mass and the body rate is adjusted to 30-60% by mass, even in non-polymer When the concentration of the catechins is high, a container-packed tea beverage which not only suppresses the bitterness but also causes a change in the composition of the catechins even after long-term storage can be obtained.

The container-packed tea beverage of the present invention contains a sufficient amount of non-polymer catechins to exhibit physiological effects, and has a reduced bitterness, so that it is easy to drink, and further excellent in flavor and composition stability even after long-term storage.

The term "non-polymer catechins" as used in the present invention refers to non-epidermal substances such as catechin, gallocatechin, catechin gallate, and gallocatechin gallate. A general term for the catechins such as the catechins, such as epicatechin, epigallocatechin, epicatechin gallate, and epigallocatechin gallate.

In the present invention, the so-called gallate body catechin refers to catechin gallate, gallic catechin gallate, epicatechin gallate, table eclipse A generic term for catechins, gallic acid esters, and the like. Further, the so-called catechin catechin refers to gallic catechin, gallic catechin gallate, epigallocatechin, epigallocatechin A general term for esters and the like.

The so-called gallate catechin rate refers to catechin gallate, gallocatechin gallate, epicatechin gallate, epigallocate The total amount of the tea gallate is relative to catechin, epicatechin, catechin gallate, epicatechin gallate, epicatechin, epigallocate The ratio of total amount of the tea, epicatechin gallate, and epigallocatechin gallate.

The packaged beverage of the present invention is obtained by blending a tea extract having a gallate body catechin content of less than 50% by mass in the non-polymer catechins in the tea extract. When the gallate body catechin rate in the non-polymer catechins of the tea extract is 50% by mass or more, the tea extract is preferably borrowed at any stage of the purification manufacturing step. The gallate-forming catechins are adjusted to less than 50% by mass by the tannase treatment or removal of a tea cream-forming product at the time of low-temperature cooling.

As the tea extract used in the present invention, an extract obtained from green tea leaves can be cited. As for the tea to be used, more specifically, a hybrid which is derived from the genus Camellia, such as Chinese tea (C. sinensis), Assam tea (C. assica), and the genus Wait for the tea obtained to make tea leaves. Among the teas made from tea, there are green teas such as sencha, fragrant tea, jade, milled tea, and steamed tea, or CTC tea. The tea extract used in the present invention is preferably one obtained by drying or concentrating an extract obtained from tea leaves of green tea or freezing it.

The extraction from the tea leaves is carried out by using water as an extraction solvent and stirring and the like. When extracting, an organic acid salt or an organic acid such as an ascorbate (for example, a sodium salt) may be added to water in advance. Further, the following method may be used in combination: boiling or degassing or introducing an inert gas such as nitrogen to remove dissolved oxygen, and extracting in a so-called non-acidified atmosphere. The extract obtained in this manner is dried and concentrated to obtain a tea extract used in the present invention. As for the form of the tea extract, there are mentioned liquid, slurry, semi-solid, and solid state.

As the tea extract, the following extract can be used: an extract obtained by extracting tea leaves subjected to supercritical carbon dioxide contact treatment. In this method, an extract containing non-polymer catechins is obtained from the tea leaves which are subjected to supercritical extraction, i.e., tea leaves.

The tea extract used in the present invention can be used for extracting from tea leaves, even if the extract extracted from the tea leaves is used instead of being dried and concentrated, and the tea extract concentrate is dissolved or diluted in water. The liquid and tea extract concentrate are used in combination.

Here, the concentrate of the tea extract refers to a concentrate which is extracted from tea leaves by using hot water or a water-soluble organic solvent, for example, Japanese Patent Laid-Open No. 59-219384, Japan The method described in the method described in Japanese Laid-Open Patent Publication No. Hei No. Hei. Specifically, as the tea extract, a commercially available "polyphenol" manufactured by Tokyo Food Techno Co., Ltd., "Taifuran" manufactured by Ito Park Co., Ltd., and "green tea polyphenol" manufactured by Sun Chemical Co., Ltd. may be used as a solid tea extract. Things.

The gallate body catechin rate is lowered by subjecting the tea extract or its concentrate to a tannase treatment. The tannase used herein is generally preferably a commercially available one having an activity of 500 to 5,000 U/g; if the enzyme activity is 500 U/g or less, sufficient activity cannot be obtained, and if the enzyme activity is 5000 U/ Above g, the enzyme reaction rate is too fast, so it becomes difficult to control the reaction system.

As the tannase, Tannin acylhydrase EC 3.1.1.20 is preferred. As for the commercial products, the product name "Citrate enzyme" Kikkoman (share) system and the tannase "Sankyo" Sangong (share) system can be cited.

As a specific method of tannase treatment, the effect of reducing the rate of non-polymer gallate catechins and stopping the enzyme reaction at the optimum non-polymer gallate catechin rate In other words, it is preferred to add tannase so as to be in the range of 0.5 to 10% by mass based on the non-polymer catechins in the green tea extract. Including the enzyme inactivation step, in order to terminate the tannase treatment within 2 hours of the optimal enzyme reaction time, the tannase concentration is preferably 0.5 to 5% by mass, more preferably 2 to 4% by mass.

The temperature of the tannase treatment is preferably from 15 to 40 ° C, more preferably from 20 to 30 ° C, for obtaining the optimum enzyme activity.

In order to terminate the tannase reaction, the enzyme activity must be inactivated. The temperature at which the enzyme is inactivated is preferably 70 to 90 ° C; the deactivation of the enzyme reaction can be carried out by heating in a batch mode or a continuous manner such as a flat type heat exchanger.

Further, after the inactivation treatment of the tannase, the tea extract can be purified by centrifugation or the like.

Further, in the case of the tea extract used in the present invention, it is preferred to carry out purification treatment before or after the tannase treatment in terms of flavor, stability and the like. As for the purification treatment method, the following methods may be used singly or in combination: (1) synthetic adsorbent treatment, (2) extraction using a mixture of an organic solvent and water, (3) activated carbon treatment, and (4) activated clay Or / and acid clay treatment, (5) solid-liquid separation and other operations.

As a synthetic adsorbent treatment, a method in which a tea extract is adsorbed to a synthetic adsorbent and then a non-polymer catechin is eluted is exemplified. More specifically, the tea extract is adsorbed to a synthetic adsorbent, and the synthetic adsorbent is washed, followed by contact with an aqueous alkaline solution to elute the non-polymer catechins. The caffeine and gallic acid can be reduced by treatment with the synthetic adsorbent. As the synthetic adsorbent to be used, styrene-divinylbenzene, modified styrene-divinylbenzene or methyl methacrylate is used as a parent. As an example of the styrene-divinylbenzene-based synthetic adsorbent, Mitsubishi Chemical Corporation's trade name is Diaion HP-20, HP-21, Sepabeads SP70, SP700, SP825, SP-825 or Organo (supplier: Amberlite XAD4, XAD16HP, XAD1180, XAD2000, Sumitomo Chemical (Supplier: Rohm and Haas Company, USA) Duolite S874, S876, etc., Rohm and Haas Company, USA. Examples of the modified styrene-divinylbenzene-based synthetic adsorbent which nucleuses a bromine atom to enhance the adsorption force include a product name Sepabeads SP205, SP206, and SP207 manufactured by Mitsubishi Chemical Corporation. Examples of the methyl methacrylate-based synthetic adsorbent include Sepabeads HP1MG manufactured by Mitsubishi Chemical Corporation, XAD7HP manufactured by HP2MG or Organo, and Duolite S877 of Sumitomo Chemical Co., Ltd., and the like.

Among the synthetic adsorbents, a polystyrene-based synthetic adsorbent and a methyl methacrylate-based synthetic adsorbent are particularly preferably modified. As a specific example of the synthetic adsorbent. For example, a modified polystyrene-based synthetic adsorbent (manufactured by Mitsubishi Chemical Corporation) such as SP207 or a methacrylic synthetic adsorbent (manufactured by Mitsubishi Chemical Corporation) such as HP2MG may be used, but SP207 is preferred for the above reasons.

The column filled with the synthetic adsorbent preferably has a SV (space velocity) of 1 to 10 [h ^-1 ] and a flow ratio of 2 to 10 [v/v] with respect to the synthetic adsorbent. The liquid conditions were washed with a 95 vol% aqueous ethanol solution to remove raw materials in the synthetic adsorbent or impurities in the raw material monomers. Then, by SV=1~10[h ^-1 ], the water is washed with a flow ratio of 1 to 10 [v/v] with respect to the synthetic adsorbent to remove the ethanol and then the synthetic adsorption. The liquid contained in the agent is replaced by a water system to enhance the adsorption energy of the non-polymer catechins.

As a method of adsorbing the tea extract to the synthetic adsorbent, it is preferred that the tea extract is passed through a column packed with a synthetic adsorbent. As a condition for passing the tea extract through a column packed with a synthetic adsorbent, it is preferred to use a flow rate of SV (space velocity) = 0.5 to 10 [h ^-1 ], relative to the synthetic adsorbent. The liquid passing ratio is 0.5 to 20 [v/v]. Further, after the green tea extract is adsorbed to the synthetic adsorbent, it is washed with water, and the conditions are set to a flow rate of SV=0.5 to 10 [h ^-1 ], and a flow ratio of 1 to 10 to the synthetic adsorbent. [v/v], removing gallic acid or impurities attached to the synthetic adsorbent.

As the alkaline aqueous solution used for the elution of the non-polymer catechins, a sodium or potassium-based alkaline aqueous solution is preferably used, and for example, an aqueous sodium hydroxide solution or an aqueous sodium carbonate solution is suitably used. Further, the pH of the alkaline aqueous solution is preferably in the range of 7 to 14. Examples of the sodium aqueous solution having a pH of 7 to 14 include a sodium hydroxide aqueous solution of 4% or less and a sodium carbonate aqueous solution of 1 N.

In the elution step, two or more kinds of eluted water having different pH values from each other are used as the eluted water. Preferably, the eluted water is brought into contact with the synthetic adsorbent in a descending order of pH. Thereby, catechin, gallocatechin, catechin gallate, gallocatechin gallate, epicatechin, epigallocatechin, Epicatechin gallate, epigallocatechin gallate. For example, as an example of using two or more kinds of eluted water having different pH values as the eluted water, after eluting with the eluted water having a pH of 3 to 7, the alkaline dissolved water having a pH of 9 to 11 can be used. Non-polymer catechins are fractionated.

Since the alkali metal salt such as a sodium salt is contained in the eluate of the non-polymer catechins by elution with an aqueous alkaline solution, it is preferred to remove the alkali metal ion by a cation exchange resin, particularly an H-type cation exchange resin. . As the cation exchange resin, specifically, Amberlite 200CT, IR120B, IR124, IR118, Diaion SK1B, SK102, PK208, PK212 and the like can be used.

In the above purification treatment, it is preferred to carry out (2) extraction with a mixed solution of an organic solvent and water and (3) treatment with activated carbon, or (2) extraction with a mixed solution of an organic solvent and water (4) The combination of activated clay or/and acid clay treatment is carried out. Further, it is particularly preferable to combine (2) extraction with a mixed solution of an organic solvent and water, (3) treatment with activated carbon, and (4) treatment with activated clay or acid clay.

In the case of extracting a mixture of an organic solvent and water, the tea extract is dispersed in a mixed solution of an organic solvent and water. The mass ratio of the organic solvent to the water in the dispersion, in terms of the extraction efficiency of the catechins, the purification of the tea extract, the long-term drinking property, and the rectification conditions for recovering the organic solvent, it is finally preferable 60/40~97/3, more preferably 60/40~75/25 or 85/15~95/5.

As the organic solvent, ethanol, methanol, acetone, ethyl acetate or the like can be mentioned. Among these, a hydrophilic organic solvent of methanol, ethanol or acetone is preferred, and in particular, ethanol is preferred in consideration of use in foods. As the water, there are mentioned, for example, ion-exchanged water, tap water, natural water, and the like. The organic solvent and water may be mixed or separately mixed with the precisely filtered tea extract, but it is preferred to prepare a mixed solution and then mix with the tea extract.

10 to 40 parts by mass, further 10 to 30 parts by mass, particularly 15 to 30 parts by mass, of the tea extract (calculated as dry mass) may be added to 100 parts by mass of the mixed solution of the organic solvent and water. It is preferable to treat the green tea extract efficiently.

More preferably, after the end of the addition of the mixed solution of the organic solvent and water, the ripening time of about 10 to 180 minutes is set.

These treatments can be carried out at 10 to 60 ° C, and particularly preferably at 10 to 50 ° C, more preferably at 10 to 40 ° C.

As for the activated carbon used in the treatment of activated carbon, there are no special restrictions if it is generally used in industrial applications. For example, ZN-50 (made by North Vietnam Carbon Co., Ltd.), Kuraraycoal GLC, Kuraraycoal PK-D, Kuraraycoal PW-D (KURARAY) Commercial products such as manufactured by CHEMICAL Co., Ltd., Astragalus AW50, Astragalus A, Astragalus M, and White C (made by Takeda Pharmaceutical Co., Ltd.).

The pore volume of the activated carbon is preferably 0.01 to 0.8 mL/g, particularly preferably 0.1 to 0.8 mL/g. Further, the specific surface area is preferably from 800 to 1600 m ² /g, particularly preferably from 900 to 1,500 m ² /g. Furthermore, the physical property values are based on the value of the nitrogen adsorption method.

The activated carbon treatment is preferably carried out after adding the green tea extract to the mixed solution of the organic solvent and water. In terms of the purification effect and the resistance of the filter cake in the filtration step, it is preferred to add 0.5 to 8 parts by mass, particularly 0.5 to 3 parts by mass, based on 100 parts by mass of the mixed solution of the organic solvent and water. Activated carbon.

Acidic clay or activated clay, which contains SiO ₂ , Al ₂ O ₃ , Fe ₂ O ₃ , CaO, MgO, etc. as a general chemical component, but the SiO ₂ /Al ₂ O ₃ ratio is preferably 3 to 12, Especially good is 4~9. Further, it is preferred to have a composition containing 2 to 5% by mass of Fe ₂ O ₃ , 0 to 1.5% by mass of CaO, and 1 to 7% by mass of MgO.

The specific surface area of the acid clay or the activated clay varies depending on the degree of acid treatment, preferably 50 to 350 m ² /g, and the pH (5 mass % suspension) is preferably 2.5 to 8, which is particularly good. It is 3.6~7. For example, commercially available products such as MIZUKA-ACE #600 (manufactured by Mizusawa Chemical Co., Ltd.) can be used as the acid clay.

Further, the ratio of the activated carbon to the acid white clay or the activated white clay may be 1 to 10 in terms of mass ratio with respect to the activated carbon 1, preferably, activated carbon: acid white clay or activated clay = 1:1 to 1: 6.

For the obtained tea extract, in order to improve the stability of the product, it is preferred to remove turbidity as needed. As for the specific operation for removing turbidity, the solid portion and the water-soluble portion are subjected to solid-liquid separation by filtration and/or centrifugation.

The conditions for solid-liquid separation are appropriately determined in such a manner as to obtain a specific turbidity. In the filtration conditions for solid-liquid separation by filtration, the temperature is preferably from 5 to 70 ° C, more preferably from 10 to 40 ° C. The pressure is preferably the pressure range of the membrane module used. For example, it is preferably 30 to 400 kPa, more preferably 50 to 400 kPa, and particularly preferably 50 to 350 kPa. The membrane pore diameter is preferably from 1 to 30 μm, more preferably from 2 to 25 μm, particularly preferably from 2 to 20 μm in terms of specific turbidity.

Further, the centrifugal separator is preferably a general machine such as a separator type, a cylinder type or a decanter type. As the centrifugal separation conditions, the temperature is preferably from 5 to 70 ° C, more preferably from 10 to 40 ° C, and the number of rotations and time are preferably adjusted to a specific turbidity. For example, in the case of a split plate type, the number of rotations is preferably 3000 to 10000 r/min, more preferably 5000 to 10000 r/min, particularly preferably 6000 to 10000 r/min; and the time is preferably 0.2. ~30 minutes, more preferably 0.2~20 minutes, especially good is 0.2~15 minutes.

For solid-liquid separation, membrane filtration is preferred. The polymer film used for membrane filtration is a hydrocarbon-based, fluorinated hydrocarbon-based or fluorene-based polymer film, and examples thereof include polyolefin-based polymer films such as polyethylene and polypropylene, and polytetrafluoroethylene (PTFE) and poly. A fluorinated polyolefin-based polymer film such as vinylidene fluoride (PVDF). Examples thereof include a fluorene-based polymer film such as polyfluorene (PSU) or polyether oxime (PES). The membrane pore diameter of the polymer membrane is 0.05 to 0.8 μm, more preferably 0.05 to 0.5 μm, and particularly preferably 0.08 to 0.5 μm. Further, as the film thickness, it is preferably 0.1 to 2.5 mm, more preferably 0.3 to 2.0 mm, and particularly preferably 0.3 to 1.5 mm.

The tea extract used in the present invention preferably contains 10 to 90% by mass, further 20 to 80% by mass, particularly 30 to 70% by mass, of the non-polymer catechins in the solid content thereof.

Further, in terms of suppressing bitterness, the rate of gallate body catechin in the tea extract must be less than 50% by mass, in terms of the effectiveness of the non-polymer catechins physiological effect and the reduction of bitterness. More preferably, it is 5 to 48% by mass, and particularly preferably 15 to 36% by mass.

The concentration of caffeine in the beverage of the present invention is preferably 0.20 or less, more preferably 0.20 or less, based on the total amount (mass ratio) of caffeine/non-polymer catechins relative to non-polymer catechins. It is 0.001 to 0.15, more preferably 0.01 to 0.14, and particularly preferably 0.05 to 0.13. When using a method that satisfies the conditions as a tea extract or a green tea extract having a high caffeine content, it can be combined with other tea extracts (catechin preparations, etc.) having a small caffeine content. The method of blending is achieved.

The container-packed tea beverage of the present invention contains 0.072 to 0.4% by mass, preferably 0.08 to 0.3% by mass, more preferably 0.09 to 0.3% by mass, still more preferably 0.1 to 0.3% by mass, dissolved in water. Non-polymer catechins in the state. When the content of the non-polymer catechins is in this range, it is easy to easily ingest a large amount of non-heavy catechins, and it is also preferable in terms of flavor and color tone stability. The concentration of the non-polymer catechins can be adjusted by the amount of the tea extract.

Further, the gallic acid content in the container-packed tea beverage of the present invention lowers the bitterness and sourness, and further preferably 21 to 150 ppm in terms of flavor and composition preservation stability. It is 25~125 ppm, especially 30~100 ppm. The gallic acid content, as described above, can be obtained by hydrolyzing the tea extract with a tannase or the like, adsorbing it to a synthetic adsorbent, and then dissolving it in an aqueous alkaline solution, or by using a tea extract. Adjust the amount and adjust it.

The body body ratio of the non-polymer catechins of the container-packed tea beverage of the present invention is preferably from 30 to 60% by mass, more preferably from 41 to 60% by mass in terms of the heat load for sterilization and the pH at the time of sterilization. ~60% by mass. Here, the body rate refers to epicatechin, epigallocatechin, epicatechin gallate, and epigallocatechin in the aforementioned non-polymer catechins. The content of gallic acid ester. The body rate can be adjusted by the pH value of the beverage, the sterilization temperature, and the sterilization time.

The container-packed tea beverage of the present invention comprises a green tea beverage, an oolong tea beverage, a black tea beverage, and a mixed beverage thereof. In order to produce the beverage, the tea extract having the rate of the aforementioned gallate body catechin can be formulated by one or more kinds of tea extracts selected from the group consisting of green tea extract, oolong tea extract, and black tea extract. And implementation.

The green tea extract is obtained by using the same green tea leaf as the raw material, and using an organic acid salt such as ascorbic acid or sodium ascorbate or an inorganic salt such as an organic acid or sodium hydrogencarbonate as water as an extraction solvent. Obtained by extraction. Further, the oolong tea extract is obtained by extracting oolong tea leaves as an raw material, and adding an organic acid salt such as ascorbic acid or sodium ascorbate or an inorganic salt such as an organic acid or sodium hydrogencarbonate as an extraction solvent in water or as an extraction solvent. The black tea extract is obtained by extracting black tea leaves as a raw material, and adding an organic acid salt such as ascorbic acid or sodium ascorbate or an inorganic salt such as an organic acid or sodium hydrogencarbonate as an extraction solvent using water or water. The plant extract such as mixed tea is not particularly limited in order to adjust the flavor, and a plant material having edible experience is used, and an organic acid salt such as ascorbic acid or sodium ascorbate or an organic acid or carbonic acid is added to water or water. An inorganic salt such as sodium hydrogen is obtained as an extraction solvent and extracted.

The pH value of the container-packed tea beverage of the present invention is preferably 5 to 7 at 20 ° C, preferably 5.5, in terms of the stability of the flavor and the chemical stability of the non-polymer catechins. ~6.9, better is 5.5~6.5.

The container-packed tea beverage of the present invention is preferred because it is easy to drink if it is formulated with a bitterness-inhibiting inhibitor. The bitter taste inhibitor to be used is not particularly limited, but an oligosaccharide or a cyclodextrin is preferred. As the cyclodextrin, α-, β-, γ-cyclodextrin and branched α-, β-, γ-cyclodextrin can be used. In the beverage, it is preferred to contain 0.005 to 0.5% by mass, and further preferably 0.01 to 0.3% by mass of the cyclodextrin. The container-packed tea beverage of the present invention may be added alone or in combination with an antioxidant, various esters, organic acids, organic acid salts, inorganic acids, inorganic acid salts, inorganic salts, coloring agents, emulsifiers, and preserved. Formulations such as seasonings, seasonings, sweeteners, sour materials, gums, emulsifiers, oils, vitamins, amino acids, vegetable extracts, nectar extracts, pH adjusters, quality stabilizers, etc.

Moreover, the container used in the container-packed tea beverage of the present invention has an oxygen permeability of 0.1 ml/day. Below bottle.

The container used for the packaged tea beverage of the present invention can be a molded container (so-called PET bottle) containing polyethylene terephthalate as a main component, a metal container such as steel or aluminum, and the like. A bottle, a metal foil, or a paper composited with a plastic film is provided in a usual form. Here, the container-packed beverage refers to a person who can drink without dilution.

The container-packed tea beverage of the present invention is produced in accordance with the sterilization conditions of the Food Sanitation Law. For example, after being filled in a container such as a metal can, it is produced by heat sterilization by distillation sterilization or the like; or for heat resistance and pressure resistance of, for example, PET bottles, resealable metal containers, paper containers, and bottle containers. If it is weak and cannot be subjected to retort sterilization, it is subjected to a high-temperature short-time sterilization in accordance with the same sterilization conditions as described above, for example, a plate heat exchanger, and then cooled to a constant temperature and then filled in a container.

Alternatively, the other components may be added to the filled container under aseptic conditions and then filled. Further, the following operations may be carried out: after heat sterilization under acidic conditions, the pH is returned to neutral under aseptic conditions; or after heat sterilization under neutral conditions, the pH is returned to acidity under aseptic conditions.

[Examples] Determination of non-polymer catechins

The container was diluted with distilled water, and filtered with a filter (0.8 μm). Then, a high-speed liquid chromatograph (model SCL-10AVP) manufactured by Shimadzu Corporation was used to install the octadecyl-incorporated liquid chromatography filling type. The column L-column TM ODS (4.6 mm Φ × 250 mm: manufactured by the Chemical Substance Evaluation Research Institute) was carried out by using a gradient method of the A liquid and the B liquid at a column temperature of 35 °C. The reaction was carried out under the following conditions: mobile phase A was a 0.1 mL/L acetic acid aqueous solution, and liquid B was a 0.1 mol/L acetic acid acetonitrile solution. The sample injection amount was 20 μL, and the UV detector wavelength was 280 nm.

Determination of caffeine

The analysis was carried out in the same manner as the non-polymer catechins.

Determination of gallic acid

The analysis was carried out in the same manner as the non-polymer catechins. Furthermore, the above gradient conditions are as follows.

Evaluation method of bitterness

The bitterness intensity is measured by the bitterness intensity test using quinine sulfate as an indicator (Reference: Perceptionad Phychophysics, 5, 1969, pp. 347-351 / JIS Z8144 / New Functional Handbook (pp. 448-449) 1990 Issued on the 9th edition of February 9th). The functional evaluation was carried out by five functional inspectors by comparing the test samples with a quinine sulfate standard solution having different bitterness intensity (10 stages) and selecting a standard solution corresponding to the bitterness intensity of the sample. The results of the functional evaluation were averaged and expressed as the bitterness intensity τ value.

Flavor stability evaluation method

The preservation stability of the flavor in the one-month preservation test at 55 ° C was evaluated.

In the flavor evaluation, the degree of deterioration is divided into three stages of large, medium, and small.

Stability evaluation method (element rate and pH value in non-polymer catechins)

The initial non-polymer catechins were measured, and the change in the body rate in the non-polymer catechins in the one-month storage test at 55 ° C was evaluated.

The difference from the body rate in the non-polymer catechins on the 0th day of storage was taken as Δ%.

(1) Tea extract (a)

The green tea extract having a catechin content of 30% was not subjected to a tannase treatment, but was spray-dried by a spray drying method. The catechins were extracted from the obtained powder in a mixed solvent of ethanol and water (water: ethanol = 40: 60), and then 8 parts by mass of activated carbon was added to the mixture for purification to obtain a tea extract (a). The gallate body catechin rate was 52% by mass.

(2) Tea extract (b)

The green tea extract having a catechin content of 30% was subjected to tannase treatment (citrate concentration: 0.5%; reaction temperature: 20 ° C, Brix of the reaction solution was 20), and spray-dried by a spray drying method. The catechins were extracted from the obtained powder in a mixed solvent of ethanol and water (water: ethanol = 40: 60), and then purified by adding 8 parts by mass of activated carbon to the mixture to obtain a tea extract (b). The gallate body catechin rate was 48% by mass.

(3) Tea extract (c)

The green tea extract having a catechin content of 30% was subjected to a tannase treatment (citrate concentration: 1.0%; reaction temperature: 20 ° C, Brix of the reaction liquid was 20), and spray-dried by a spray drying method. The catechins were extracted from the obtained powder by a mixed solvent of ethanol and water (water: alcohol = 40:60), and then purified by adding 8 parts by mass of activated carbon to the mixture to obtain a tea extract (c). The gallate body catechin rate was 32% by mass.

(4) Tea extract (d)

The green tea extract having a catechin content of 30% was subjected to a tannase treatment (captanase concentration: 2.0%; reaction temperature: 20 ° C, Brix of the reaction liquid was 20), and spray-dried by a spray drying method. The catechins were extracted from the obtained powder by a mixed solvent of ethanol and water (water: ethanol = 40: 60), and then 8 parts by mass of activated carbon was added to the mixture for purification to obtain a tea extract (d). The gallate body catechin rate was 2% by mass.

(5) Tea extract (e)

The green tea extract having a catechin content of 30% was subjected to a tannase treatment (captanase concentration: 2.0%; reaction temperature: 20 ° C, Brix of the reaction liquid was 20), and spray-dried by a spray drying method. Next, the obtained powder was diluted with water so that the catechin concentration became 1%. 1 Kg of a synthetic adsorbent (SP70; Diaion) was added to 4 Kg of the powder to adsorb the catechins to the synthetic adsorbent. Thereafter, the green tea extract residue in the synthetic adsorbent is washed with water. The catechins were extracted with a mixed solvent of ethanol and water (water: ethanol = 50:50) with respect to 1 kg of the washed SP70, and then 30 parts by mass of activated carbon was added to the mixture for purification to obtain a tea extract. (e). The gallate body catechin rate was 8% by mass.

Tea extract production method and analytical value

Add 33.3 g of green tea leaves to water at 65 °C in 1000 g, stir for 30 seconds at 250 r/min, hold for 90 seconds, then stir at 250 r/min for 10 seconds, hold for 170 seconds, filter, and obtain 916 g green tea extract. The non-polymer catechin concentration in the green tea extract was 0.2% by mass, the gallate body catechin ratio was 46% by mass, the caffeine concentration was 0.06% by mass, and the gallic acid amount was 0.001% by mass.

The obtained green tea extract was used in the tea extracts of Examples 2 and 3 and Comparative Example 1. Further, the obtained green tea extract was passed through a column packed with resin SP70 (300 g manufactured by Mitsubishi Chemical Corporation) at a flow rate of about 25 ml/min, and the obtained tea extract was used in Example 1.

The black tea extract of Example 4 was prepared by adding 33.3 g of black tea leaves to 1000 g of water at 65 ° C, stirring at 250 r/min for 30 seconds, holding for 90 seconds, and then stirring at 250 r/min for 10 seconds. After maintaining for 170 seconds, it was filtered to obtain 892 g of black tea extract. The obtained black tea extract was passed through a column packed with resin SP70 (300 g manufactured by Mitsubishi Chemical Corporation) at a flow rate of about 25 ml/min, and the obtained tea extract was used in Example 4.

The oolong tea extract of Example 5 is obtained by adding 33.3 g of oolong tea leaves to 1000 g of water at 65 ° C, stirring at 250 r/min for 30 seconds, holding for 90 seconds, and then stirring at 250 r/min for 10 seconds. After maintaining for 170 seconds, it was filtered to obtain 933 g of oolong tea extract. The obtained black tea extract was passed through a column packed with resin SP70 (300 g manufactured by Mitsubishi Chemical Corporation) at a flow rate of about 25 ml/min, and the obtained tea extract was used in Example 5.

Examples 1 to 5 and Comparative Example 1

A tea beverage (pH 6) was produced in the places shown in Tables 1 and 2. The beverage was sterilized by 138 ° C, 30 seconds high temperature short time sterilization, and filled with oxygen permeability of 0.03 mL / day. A transparent PET bottle in a bottle (measured by an oxygen permeability measuring device manufactured by MOCON Corporation).

As can be seen from Tables 1 and 2, the beverage having a gallate-free catechin content of more than 50% by mass has a bitter taste and changes in flavor and body rate due to storage. It is understood that the beverage having a gallate-free catechin rate of less than 50% by mass can suppress the initial bitterness, and even if it is stored, the flavor and the body body ratio are hard to change.

Further, gallic acid was added to the tea beverage of Example 1, and the flavor was evaluated. When the concentration of gallic acid was 50 ppm or 150 ppm, there was no problem in flavor. However, when the concentration of gallic acid is 200 ppm or more, it is found that odor is generated.

Claims (5)

A container-packed tea beverage prepared by blending a tea extract with a tea extract, wherein the tea extract is treated with tannase to convert the non-polymer catechins to gallate catechins Adjusted to 36% by mass or less, the container-packed tea beverage contains: (A) 0.072 to 0.4% by mass of non-polymer catechins, and (B) 10 to 150 ppm of gallic acid, and non-polymer The catechins of catechins have a catechin content of 0 to 36% by mass and a body body ratio of 30 to 60% by mass. The container-packed tea beverage according to claim 1, wherein the tea extract is one or more selected from the group consisting of a green tea extract, an oolong tea extract, and a black tea extract. The container-packed tea beverage according to claim 1, wherein the pH value measured at a liquid temperature of 20 ° C is 5-7. The container-packed tea beverage according to any one of claims 1 to 3, wherein a ratio of the total amount of caffeine to non-polymer catechins contained in the beverage is 0.2 or less. The container-packed tea beverage according to any one of claims 1 to 3, wherein the container has an oxygen permeability of 0.1 mL/day. Bottle below.