JP7332610B2 - タンパク質の精製条件の決定 - Google Patents

タンパク質の精製条件の決定 Download PDF

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JP7332610B2
JP7332610B2 JP2020544212A JP2020544212A JP7332610B2 JP 7332610 B2 JP7332610 B2 JP 7332610B2 JP 2020544212 A JP2020544212 A JP 2020544212A JP 2020544212 A JP2020544212 A JP 2020544212A JP 7332610 B2 JP7332610 B2 JP 7332610B2
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protein
proteins
stationary phase
data
yield
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JP2021521412A (ja
JP2021521412A5 (enExample
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シェイヴァー，ジェレミー，マーティン
アミムー，タイルリ
ギレスピー，ロン
ケッチェム，ランダル，アール．
ガルシア，フェルナンド
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ジャスト－エヴォテック バイオロジクス，インコーポレイテッド
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8693—Models, e.g. prediction of retention times, method development and validation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8658—Optimising operation parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20—Protein or domain folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • G16B5/20—Probabilistic models
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/889—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 monitoring the quality of the stationary phase; column performance

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Biophysics (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Evolutionary Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Probability & Statistics with Applications (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2020544212A 2018-02-21 2019-02-21 タンパク質の精製条件の決定 Active JP7332610B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862633584P 2018-02-21 2018-02-21
US62/633,584 2018-02-21
PCT/US2019/019050 WO2019165148A1 (en) 2018-02-21 2019-02-21 Determining conditions for purification of proteins

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JP2021521412A JP2021521412A (ja) 2021-08-26
JP2021521412A5 JP2021521412A5 (enExample) 2022-03-02
JP7332610B2 true JP7332610B2 (ja) 2023-08-23

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US (1) US20200408728A1 (enExample)
EP (2) EP3755447B1 (enExample)
JP (1) JP7332610B2 (enExample)
CA (1) CA3091905C (enExample)
ES (1) ES2978413T3 (enExample)
WO (1) WO2019165148A1 (enExample)

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MY204234A (en) 2018-08-27 2024-08-16 Regeneron Pharma Use of raman spectroscopy in downstream purification
WO2021216635A1 (en) * 2020-04-23 2021-10-28 Amgen Inc. Selecting chromatography parameters for manufacturing therapeutic proteins
WO2022060535A1 (en) * 2020-09-21 2022-03-24 Bayer Healthcare Llc Pathogen clearance system and method
EP4780828A2 (en) * 2023-09-18 2026-07-29 Regeneron Pharmaceuticals, Inc. Methods and systems for developing chromatography protocols
CN121075496B (zh) * 2025-11-05 2026-03-27 鲁东大学 一种基于特征工程与集成学习的蛋白质盐敏感性预测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003530572A (ja) 2000-04-11 2003-10-14 ザ プロクター アンド ギャンブル カンパニー 高速液体クロマトグラフィー・パラメータのモデル化、予測、及び最適化のための方法
US20060096924A1 (en) 2002-09-19 2006-05-11 Hartmut Schlueter Method for discovering suitable chromatography conditions for the separation of biological molecules
JP2009539931A (ja) 2006-06-14 2009-11-19 ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 生体分子のクロマトグラフィー精製プロセスの最適化方法
US20160367911A1 (en) 2015-06-16 2016-12-22 Ge Healthcare Bio-Sciences Ab Determination of chromatography conditions
JP2017534060A (ja) 2014-11-12 2017-11-16 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体クロマトグラフィープロトコルにおける実験パラメータの影響を決定するための方法及びシステム

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040034477A1 (en) * 2002-08-19 2004-02-19 Mcbrien Michael Methods for modeling chromatographic variables

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003530572A (ja) 2000-04-11 2003-10-14 ザ プロクター アンド ギャンブル カンパニー 高速液体クロマトグラフィー・パラメータのモデル化、予測、及び最適化のための方法
US20060096924A1 (en) 2002-09-19 2006-05-11 Hartmut Schlueter Method for discovering suitable chromatography conditions for the separation of biological molecules
JP2009539931A (ja) 2006-06-14 2009-11-19 ベーリンガー インゲルハイム ファルマ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 生体分子のクロマトグラフィー精製プロセスの最適化方法
JP2017534060A (ja) 2014-11-12 2017-11-16 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 液体クロマトグラフィープロトコルにおける実験パラメータの影響を決定するための方法及びシステム
US20160367911A1 (en) 2015-06-16 2016-12-22 Ge Healthcare Bio-Sciences Ab Determination of chromatography conditions

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EP3755447A1 (en) 2020-12-30
JP2021521412A (ja) 2021-08-26
CA3091905C (en) 2023-03-21
EP4372376A2 (en) 2024-05-22
US20200408728A1 (en) 2020-12-31
EP3755447B1 (en) 2024-03-06
EP4372376A3 (en) 2024-07-17
EP4372376B1 (en) 2026-04-01
ES2978413T3 (es) 2024-09-12
WO2019165148A1 (en) 2019-08-29
CA3091905A1 (en) 2019-08-29

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