JP7310968B2 - Analysis support method and analysis support device - Google Patents

Description

The present invention relates to an analysis support method and an analysis support apparatus for supporting determination of analysis conditions in supercritical fluid chromatography.

A supercritical fluid chromatograph (SFC) using a supercritical fluid as a mobile phase is known. In the analysis device described in Patent Document 1, an analysis column is provided in the first flow path, and a first back pressure control valve is provided downstream of the analysis column. A second flow path branching from the first flow path is provided, and a second back pressure regulating valve is provided in the second flow path. A portion of the sample and mobile phase introduced into the supercritical fluid chromatograph is introduced into the analytical column of the first channel, and the remaining sample and mobile phase are discharged through the second channel.

WO2016/031008

In the analyzer described in Patent Document 1, the amount of sample supplied to the first flow path and the second flow rate are adjusted by adjusting the set values of the pressures of the first back pressure regulating valve and the second back pressure regulating valve. The ratio to the amount of sample discharged from the channel (hereinafter referred to as sample introduction ratio) can be adjusted. Thereby, the amount of sample introduced into the analytical column can be adjusted.

However, even if the set values of the pressures of the first back pressure control valve and the second back pressure control valve are constant, the sample introduction rate fluctuates due to the pressure loss caused by the piping and analysis column used in the analyzer. Since fluctuations in the sample introduction ratio affect detection sensitivity and analysis accuracy, the sample introduction ratio is adjusted to the value specified in the analysis method indicating the analysis conditions by adjusting the pressure setting value of the first back pressure adjustment valve. It is necessary to. In this case, after confirming that the sensitivity of the detector of the analyzer is constant, analysis is performed to adjust the sample introduction ratio, and the peak intensity obtained by the detector is used as an index to obtain the first The pressure setting of the back pressure regulating valve must be adjusted. This makes setup for analysis laborious and time consuming.

SUMMARY OF THE INVENTION An object of the present invention is to provide an analysis support method and an analysis support apparatus that enable the sample introduction ratio to be adjusted easily and reliably.

An analysis support method according to one aspect of the present invention includes a liquid feeding unit that supplies a mobile phase containing a supercritical fluid to a supply channel, and first and second channels that branch from the supply channel. and a second back pressure regulator, and an analysis column provided upstream of the first back pressure regulator in the first flow path. and directed the supercritical fluid chromatograph to feed the mobile phase into the feed channel at a flow rate of the mobile phase fed into the first channel based on a predefined total flow rate and sample introduction rate. and detecting a first set value at which the mobile phase is no longer supplied to the second channel, among the set values of the pressure of the first back pressure regulator .

According to the analysis support method, the set value of the pressure of the first back pressure regulator when the mobile phase is no longer supplied to the second channel is supplied to the first channel at a predetermined sample introduction rate. It corresponds to a first setpoint for supplying a predetermined flow rate of the mobile phase. Therefore, the set value of the pressure of the first back pressure regulator for obtaining the desired sample introduction rate can be determined in a short time without analysis. Also, the pressure setting of the first back pressure regulator can be determined without being influenced by other factors such as the sensitivity of the detector. Therefore, it becomes possible to easily and reliably adjust the sample introduction ratio.

The analysis support method may further comprise setting the pressure of the first backpressure regulator to the detected first set point. In this case, the pressure of the first backpressure regulator is automatically set to obtain the defined sample introduction rate.

The analysis support method includes a step of storing analysis conditions including a prescribed sample introduction ratio, a prescribed total flow rate of the mobile phase and a prescribed second set value; determining or updating as part of . In this case, it is possible to easily create and update analysis methods that define analysis conditions for samples.

The analysis support method may further comprise presenting an alert if the sensed first backpressure regulator pressure set point drops below a predetermined threshold. In this case, it is possible to inform the user of the occurrence of trouble such as clogging of the first or second channel.

An analysis support device according to another aspect of the present invention includes a liquid feeding unit that supplies a mobile phase containing a supercritical fluid to a supply channel; An analysis support device that supports determination of analysis conditions for a supercritical fluid chromatograph, including first and second back pressure regulators, and an analysis column provided upstream of the first back pressure regulator in the first flow path. and instructing the supercritical fluid chromatograph to feed the mobile phase into the feed channel at a flow rate of the mobile phase fed into the first channel based on a predetermined total flow rate and sample introduction rate and a detection unit for detecting a first set value at which the mobile phase is no longer supplied to the second channel, among the set values of the pressure of the first back pressure regulator .

According to the analysis support device, it becomes possible to automatically and easily and reliably adjust the sample introduction ratio.

The analysis support device may further include a setting unit that sets the pressure of the first back pressure regulator to the detected first set value. In this case, the pressure of the first backpressure regulator is automatically set to obtain the defined sample introduction rate.

The analysis support device includes a storage unit that stores analysis conditions including a prescribed sample introduction ratio, a prescribed total flow rate of the mobile phase, and a prescribed second set value, and analyzes the detected first set value. A determination updater that determines or updates as part of the condition may further be provided. In this case, it is possible to easily create and update analysis methods that define analysis conditions for samples.

The analysis support device may further include a presentation unit that presents a warning when the set value of the pressure of the first back pressure regulator detected by the detection unit falls below a predetermined threshold value. In this case, it is possible to inform the user of the occurrence of trouble such as clogging of the first or second channel.

ADVANTAGE OF THE INVENTION According to this invention, it becomes possible to adjust a sample introduction ratio easily and reliably.

1 is a block diagram showing the configuration of an analysis system including an analysis support device according to an embodiment of the present invention; FIG. 2 is a schematic diagram showing the configuration of a supercritical fluid chromatograph included in the analysis system of FIG. 1. FIG. 2 is a block diagram showing a functional configuration of the analysis support device of FIG. 1; FIG. It is a flowchart which shows the algorithm of an analysis support program.

Hereinafter, an analysis support method, an analysis support device, an analysis support program, and an analysis system according to embodiments of the present invention will be described in detail with reference to the drawings.

(1) Configuration of Analysis System FIG. 1 is a block diagram showing the configuration of an analysis system including an analysis support device according to an embodiment of the present invention. FIG. 2 is a schematic diagram showing the configuration of a supercritical fluid chromatograph included in the analysis system of FIG.

An analysis system 100 in FIG. 1 includes a supercritical fluid chromatograph 1 and an analysis support device 3 . First, the supercritical fluid chromatograph 1 shown in FIG. 2 will be described. In this embodiment, CO ₂ (carbon dioxide) is used as the supercritical fluid.

The supercritical fluid chromatograph 1 includes a liquid feeder 110, a supercritical fluid extractor 120, an autosampler 130, a column oven 140, a detector 150, and a first back pressure regulator (hereinafter referred to as BPR). ) 161 , a second back pressure regulator (hereinafter referred to as BPR) 162 , and a make-up pump 170 . In the present embodiment, supercritical fluid extraction section 120 and autosampler 130 constitute sample introduction section 200 .

Liquid delivery unit 110 includes CO ₂ pump 111 , modifier pump 112 and mixer 113 . The CO ₂ pump 111 draws CO ₂ from the cylinder 114 while pressurizing it. The CO ₂ pump 111 has a pressure gauge for detecting pressure on the discharge side and a monitor for displaying the pressure value. Modifier pump 112 draws a modifier, which is a polar solvent, from modifier container 115 . Methanol or ethanol, for example, are used as modifiers.

The mixer 113 mixes the CO ₂ drawn out by the CO ₂ pump 111 and the modifier drawn out by the modifier pump 112, and supplies the mixture as a mobile phase to the supply flow path PA. In this embodiment, mixer 113 is a gradient mixer that mixes _CO2 and modifiers in a set ratio.

A supercritical fluid extractor 120 is provided in the supply channel PA. The supercritical fluid extraction unit 120 includes an extraction vessel 121 containing a sample. The supercritical fluid extractor 120 extracts the component to be analyzed from the sample as a sample component by using the supercritical fluid (CO ₂ in the present embodiment) contained in the mobile phase when the sample is analyzed. The extracted sample component and mobile phase are supplied to supply channel PA.

Autosampler 130 includes needle 131 , injection port 132 , switching valve 133 and sample loop 134 . Needle 131 sucks the standard sample and discharges it to injection port 132 . Sample loop 134 temporarily holds the standard sample injected into injection port 132 . A supply channel PA, a supply channel PB, an injection port 132 and a sample loop 134 are connected to the switching valve 133 . The switching valve 133 connects the first state in which the supply channel PA is connected to the supply channel PB and the injection port 132 is connected to the sample loop 134, and the supply channel PA, the sample loop 134, and the supply channel PB. and a second state.

The supply channel PB is branched into a first channel P1 and a second channel P2 at a branch portion N1. Column oven 140 houses analytical column 141 and maintains analytical column 141 at a set temperature. An analysis column 141, a detector 150 and a first BPR 161 are provided in this order in the first flow path P1. A sample component and a mobile phase are introduced into the analysis column 141 . The analysis column 141 further separates the sample components by component. The separated components are detected by detector 150 . Detector 150 is, for example, an ultraviolet detector.

The first BPR 161 operates to maintain the suction side pressure (in this embodiment, the pressure downstream of the detector 150) at a setpoint. The pressure downstream of the first BPR 161 is atmospheric pressure. The makeup pump 170 draws the makeup liquid out of the makeup container 171 and supplies it to the junction N2 with the first flow path P1.

A second BPR 162 is provided in the second flow path P2. The second BPR 162 operates to maintain the suction side pressure (in this embodiment, the pressure of the second flow path P2) at a set value. A flow sensor 163 is provided at the drain of the second BPR 162 . The flow rate sensor 163 is used to detect when the mobile phase is no longer discharged from the drain of the second BPR 162 (when the flow rate of the mobile phase becomes 0). Flow sensor 163 may not be provided. In that case, the user visually detects that the mobile phase is no longer discharged from the drain or that the second BPR 162 is closed.

By adjusting the pressure setpoint of the first BPR 161 and the pressure setpoint of the second BPR 162, the _CO2 in the supply channels PA, PB, the first channel P1 and the second channel P2 It becomes a supercritical state. Further, by adjusting the set value of the pressure of the first BPR 161 and the set value of the pressure of the second BPR 162, the flow rate of the sample introduced into the first flow path P1 and the flow rate of the sample introduced into the second flow path P2 The ratio of the sample flow rate (hereinafter referred to as the sample introduction ratio) changes. The sample introduction ratio corresponds to the ratio between the flow rate of the mobile phase supplied to the first flow path P1 and the flow rate of the mobile phase supplied to the second flow path P2. The sample introduction ratio is also called the split ratio. For example, it may be preferable that the amount of sample introduced into the supercritical fluid extraction unit 120 is large, and that the amount of sample introduced into the analysis column 141 is relatively small. In such a case, it is possible to adjust the flow rate of the sample introduced into the supercritical fluid extraction unit 120 and the analysis column 141 to appropriate values by adjusting the sample introduction ratio.

As shown in FIG. 1, the supercritical fluid chromatograph 1 includes a control device 180. As shown in FIG. Controller 180 controls CO ₂ pump 111 , modifier pump 112 , mixer 113 , supercritical fluid extraction section 120 and autosampler 130 . Controller 180 also controls column oven 140 , first BPR 161 , second BPR 162 , detector 150 and make-up pump 170 . Further, the control device 180 obtains the pressure value from the pressure gauge of the CO ₂ pump 111 and the flow rate value detected by the flow rate sensor 163 .

The analysis support device 3 includes an input/output I/F (interface) 31, a CPU (central processing unit) 32, a RAM (random access memory) 33, a ROM (read only memory) 34 and a storage device 35. For example, a personal computer or configured by the server. Input/output I/F 31 , CPU 32 , RAM 33 , ROM 34 and storage device 35 are connected to bus 38 . An operation unit 36 and a display unit 37 are connected to the bus 38 of the analysis support device 3 . The operation unit 36 includes a keyboard, pointing device, etc., and is used for inputting various values and performing various operations. The display unit 37 includes a liquid crystal display, an organic electroluminescence display, or the like, and displays various information and images. The operation unit 36 and the display unit 37 may be composed of a touch panel display.

The storage device 35 includes a storage medium such as a hard disk, an optical disk, a magnetic disk, a semiconductor memory, or a memory card, and stores an analysis support program. A RAM 33 is used as a work area for the CPU 32 . A system program is stored in the ROM 34 . The CPU 32 executes an analysis support program stored in the storage device 35 on the RAM 33 to implement an analysis support method, which will be described later.

(2) Method for Adjusting Sample Introduction Ratio A method for adjusting the sample introduction ratio will be described below. As the sample analysis method, the sample introduction ratio, the type of the analytical column 141 (the inner diameter of the analytical column, the length of the analytical column and the packing material), the total flow rate of the mobile phase, the type of modifier, the concentration of the modifier, the amount of makeup liquid The type and pressure set point of the second BPR 162, etc. are specified in the analysis method. The supply channel PA and the supply channel PB are connected through a switching valve 133 .

First, the user mounts the analysis column 141 and modifier container 115 specified in the analysis method on the supercritical fluid chromatograph 1 . Next, the user or the analysis support device 3 sets the pressure of the second BPR 162 to the second set value specified in the analysis method. Also, if necessary, the user attaches to the supercritical fluid chromatograph 1 a makeup container 171 containing a makeup liquid specified in the analysis method.

In this state, the pressure of the first BPR 161 is set to a value higher than the second set value by the user's operation of the operation unit 36 or the instruction of the analysis support device 3 . Further, according to the operation of the operation unit 36 by the user or the instruction of the analysis support device 3, the liquid supply unit 110 supplies the mobile phase with the modifier concentration specified at a predetermined flow rate to the supply channels PA and PB. Here, the predetermined flow rate is theoretically supplied to the first flow path P1 when the mobile phase is supplied to the supply flow paths PA and PB at a predetermined total flow rate and a predetermined sample introduction ratio. is the phase flow rate. For example, when the total flow rate of the mobile phase is 5 mL / min and the sample introduction rate is 5%, the mobile phase is theoretically supplied to the first flow path P1 at a flow rate of 0.25 mL / min. . Therefore, the liquid sending unit 110 supplies the mobile phase to the supply channels PA and PB at a flow rate of 0.25 mL/min. Initially, the mobile phase is supplied to the first channel P1 and the second channel P2.

The set value of the pressure of the first BPR 161 is decreased by a constant value (for example, 0.1 MPa) by the user's operation of the operation unit 36 or the instruction of the analysis support device 3 . As a result, the flow rate of the mobile phase in the second flow path P2 gradually decreases.

The analysis support apparatus 3 detects the setting value of the pressure of the first BPR 161 when the flow rate of the mobile phase detected by the flow rate sensor 163 becomes 0 as the first setting value. Alternatively, the user can set the pressure setting value of the first BPR 161 when the mobile phase is no longer discharged from the drain of the second BPR 162 or when the second BPR 162 is in a closed state as the first setting value. input. The difference between the first set value of the pressure of the first BPR 161 and the second set value of the pressure of the second BPR 162 in this case is the predetermined flow rate (the defined total flow rate and the defined sample It is the pressure difference when the mobile phase is introduced at the flow rate theoretically calculated from the introduction ratio).

Thus, the first set value of the pressure of the first BPR 161 for supplying the mobile phase at the specified total flow rate to the first BPR 161 and the second BPR 162 at the specified sample introduction ratio is It is determined.

(3) Functional Configuration of Analysis Support Device 3 FIG. 3 is a block diagram showing the functional configuration of the analysis support device 3 of FIG. As shown in FIG. 3, the analysis support device 3 includes a condition acquisition unit 301, a condition setting unit 302, a control instruction unit 303, a setting value detection unit 304, a storage unit 305, a presentation unit 306, and a determination update unit 307. The functions of the components (301 to 307) described above are realized by the CPU 32 of FIG. A part or all of the constituent elements of the analysis support device 3 may be realized by hardware such as an electronic circuit.

The storage unit 305 stores one or more analysis methods. The condition acquisition unit 301 acquires analysis conditions such as various set values input by the user using the operation unit 36 or analysis conditions defined by analysis methods stored in the storage unit 305 . The condition setting unit 302 sets each unit of the supercritical fluid chromatograph 1 via the control device 180 of the supercritical fluid chromatograph 1 based on the analysis conditions acquired by the condition acquiring unit 301 . The control instruction unit 303 instructs the control device 180 to control each unit of the supercritical fluid chromatograph 1 based on various instructions input by the user using the operation unit 36 or analysis conditions acquired by the condition acquisition unit 301. do.

The set value detection unit 304 detects the set value of the pressure of the first BPR 161 when the mobile phase stops flowing to the drain of the second BPR 162 as the first set value. Specifically, the set value detection unit 304 detects the set value of the pressure of the first BPR 161 when the flow rate of the mobile phase detected by the flow sensor 163 becomes 0 as the first set value. Alternatively, the user visually checks the set value of the pressure of the first BPR 161 when the mobile phase is no longer discharged from the drain of the second BPR 162 or when the second BPR 162 is closed, and changes the set value to the operation unit 36. may be input as the first set value. In this case, the set value detection unit 304 detects the input first set value.

The determination update unit 307 determines the first set value of the pressure of the first BPR 161 detected by the set value detection unit 304 as part of the analysis conditions of the analysis method in the storage unit 306, or defines it in the analysis method. The set value of the pressure of the first BPR 161 received is updated to the first set value. The condition setting section 302 sets the first set value detected by the set value detection section 304 to the first BPR 161 of the supercritical fluid chromatograph 1 via the control device 180 .

The presentation unit 306 determines whether or not the set value of the pressure of the first BPR 161 is lower than a predetermined threshold value in the process of decreasing the set value of the pressure of the first BPR 161 . is lower than the threshold value, the display unit 37 presents a warning to the user indicating the occurrence of trouble such as clogging of the first or second flow paths P1, P2. In this case, the control instruction section 303 stops the operation of the supercritical fluid chromatograph 1 .

(4) Analysis Support Program FIG. 4 is a flow chart showing the algorithm of the analysis support program. The algorithm of the analysis support program will be described with reference to FIG. The analysis support method is carried out by executing the analysis support program.

First, the condition setting unit 302 sets the pressure of the second BPR 162 to the second set value specified in the analysis method (step S1). Specifically, the user may use the operator 36 to input a second set value for the pressure of the second BPR 162 . In this case, the condition acquisition unit 301 acquires the input second setting value, and the condition setting unit 302 sets the acquired second setting value in the second BPR 162 via the control device 180 . Further, the condition acquisition unit 301 acquires the set value of the pressure of the second BPR 162 defined in the analysis method in the storage unit 305 as the second set value, and the condition setting unit 302 acquires the acquired second set value. may be set to second BPR 162 via controller 180 . Alternatively, the user may directly set the second setpoint in the second BPR 162 of the supercritical fluid chromatograph 1 .

Next, the condition setting unit 302 sets the pressure of the first BPR 161 to a predetermined value higher than the second set value of the pressure of the second BPR 162 via the control device 180 (step S2). Also, the condition setting unit 302 sets the modifier concentration in the liquid sending unit 110 via the control device 180 (step S3). Specifically, the user uses the operation unit 36 to input a specified modifier density. In this case, the condition acquisition unit 301 acquires the input modifier concentration, and the condition setting unit 302 sets the acquired modifier concentration in the liquid sending unit 110 . Alternatively, the condition setting unit 302 may set the modifier concentration in the liquid sending unit 110 based on the analysis method stored in the storage unit 305 .

Next, the control instruction unit 303 instructs the control device 180 to supply the mobile phase to the supply channel PA at a predetermined flow rate calculated from the specified total flow rate and the specified sample introduction ratio (step S4). Thereby, the control device 180 controls the liquid sending section 110 so that the mobile phase is supplied to the supply channel PA at a predetermined flow rate.

After that, the control instruction unit 303 instructs the control device 180 to decrease the set value of the pressure of the first BPR 161 by a constant value (for example, 0.1 MPa) (step S5). As a result, the control device 180 decreases the set value of the pressure of the first BPR 161 by a constant value.

The presentation unit 306 determines whether or not the set value of the pressure of the first BPR 161 that is lowered by the instruction from the control instruction unit 303 is equal to or greater than the threshold value (step S6). In this case, the presentation unit 306 may acquire the set value of the pressure of the first BPR 161 based on an instruction to lower the set value by the control instruction unit 303, or may obtain the set value of the pressure of the first BPR 161 from the control device 180. You can get the setting value. Here, the threshold value is a threshold value for determining whether or not a problem such as clogging has occurred in the first or second flow paths P1, P2, and is, for example, 8 MPa.

When the set value of the pressure of the first BPR 161 is equal to or higher than the threshold value, the set value detection unit 304 determines whether the flow rate of the mobile phase discharged from the drain of the second BPR 162 is 0. (step S7). When the flow rate of the mobile phase discharged from the drain of the second BPR 162 is not 0, the control instructing section 303 returns to step S5 and repeats the processing of steps S5 to S7.

When the flow rate of the mobile phase discharged from the drain of the second BPR 162 becomes 0, the set value detection unit 304 detects the set value of the pressure of the first BPR 161 as the first set value (step S8). The detected first setting value is stored in the storage unit 305 . The decision update unit 307 decides or updates the first setting value as part of the analysis conditions of the analysis method (step S9). Specifically, the determination updating unit 307 determines the first setting value as part of the analysis conditions of the analysis method stored in the storage unit 305 . Alternatively, if the analysis method defines a set value for the pressure of the first BPR 161, the determination updating unit 307 updates the defined set value to the first set value. The condition setting unit 302 sets the pressure of the first BPR 161 to the first set value detected by the set value detection unit 304 (step S10).

When the set value of the pressure of the first BPR 161 is lower than the threshold in step S6, the presentation unit 306 presents a warning to the user through the display unit 37 (step S11). Further, the control instruction unit 303 instructs the control device 180 to stop the operation of the supercritical fluid chromatograph 1 (step S12).

(5) Effect of the Embodiment According to the present embodiment, the pressure of the first BPR 161 is set to the second set value while the pressure of the second BPR 162 is set to the predetermined second set value. set to a higher value than In this state, the flow rate of the mobile phase theoretically supplied to the first flow path P1 when the mobile phase is supplied to the supply flow paths PA and PB at a predetermined total flow rate and a predetermined sample introduction ratio , the mobile phase is supplied to the supply channels PA and PB. In this case, the mobile phase is supplied to at least the second flow path P2. Thereafter, the pressure setpoint of the first BPR 161 is gradually decreased. As a result, the flow rate of the mobile phase supplied to the first flow path P1 increases, and the flow rate of the mobile phase supplied to the second flow path P2 decreases. The set value of the pressure of the first BPR 161 when the mobile phase is no longer supplied to the second flow path P2 is detected as the first set value. The first set value corresponds to the pressure set value of the first BPR 161 for supplying a predetermined flow rate of the mobile phase to the first channel P1 at a predetermined sample introduction rate. Therefore, the setting value of the pressure of the first BPR 161 for obtaining the desired sample introduction ratio can be determined in a short time without analysis. Also, the pressure setting of the first BPR 161 can be determined without being affected by other factors such as the sensitivity of the detector 150 . Therefore, it is possible to easily and reliably adjust the sample introduction ratio.

It is also possible to easily create and update analytical methods that include sample introduction rates, first setpoints for first BPR 161 pressure, and second setpoints for second BPR 162 pressure for various samples. Become.

Furthermore, based on the warning presented by the display unit 37, the user can easily understand that a problem such as clogging has occurred in the first or second flow path P1 or P2.

(6) Other Embodiments (a) The user may directly set some of the values of the multiple analysis conditions in the relevant portion of the supercritical fluid chromatograph 1 .

(b) In the above embodiment, sample introduction unit 200 includes supercritical fluid extraction unit 120 and autosampler 130, but sample introduction unit 200 may include either supercritical fluid extraction unit 120 or autosampler 130. good.

(7) Correspondence between each constituent element of the claims and each element of the embodiment An example of correspondence between each constituent element of the claim and each element of the embodiment will be described below. is not limited to In the above embodiments, the condition setting unit 302 is an example of the setting unit, the control instruction unit 303 is an example of the instruction unit, and the set value detection unit 304 is an example of the detection unit. Various other elements having the structure or function described in the claims can be used as each component of the claims.

DESCRIPTION OF SYMBOLS 1... Supercritical fluid chromatograph, 3... Analysis support apparatus, 31... Input/output I/F, 32... CPU, 33... RAM, 34... ROM, 35... Storage device, 36... Operation unit, 37... Display unit, 38 ... bus, 100... analysis system, 110... solution sending part, 111... CO2 pump, 112... modifier pump, 113... mixer, 114... cylinder, 115... modifier container, 120... supercritical fluid extraction part, 121... extraction Vessel 130 Autosampler 131 Needle 132 Injection port 133 Switching valve 134 Sample loop 140 Column oven 141 Analysis column 150 Detector 161, 162 BPR 163 Flow rate Sensor 170 Makeup pump 171 Makeup container 180 Control device 200 Sample introduction unit 301 Condition acquisition unit 302 Condition setting unit 303 Control instruction unit 304 Setting value detection unit 305...storage part, 306...presentation part, 307...determination update part, N1...branch part, N2...merge part, P1, P2... flow path, PA, PB... supply flow path

Claims (8)

a liquid feeder for supplying a mobile phase containing a supercritical fluid to a supply channel; first and second back pressure regulators respectively provided in first and second channels branching from the supply channel; An analysis support method for supporting determination of analysis conditions for a supercritical fluid chromatograph including an analysis column provided upstream of the first back pressure regulator in the first flow path,
After instructing the supercritical fluid chromatograph to supply mobile phase to the feed channel at a flow rate of the mobile phase supplied to the first channel based on a predetermined total flow rate and sample introduction rate. and a step of detecting a first set value at which the mobile phase is no longer supplied to the second channel, among the set values of the pressure of the first back pressure regulator . 2. The analysis support method of claim 1, further comprising setting the pressure of the first backpressure regulator to the detected first set point. storing analysis conditions including a defined sample introduction rate, a defined total flow rate of mobile phase and a defined second set point;
3. The analysis support method according to claim 1, further comprising the step of determining or updating said detected first setting value as part of analysis conditions. 4. The method of any one of claims 1 to 3, further comprising presenting a warning when the sensed first backpressure regulator pressure setpoint drops below a predetermined threshold. analysis support method. a liquid feeder for supplying a mobile phase containing a supercritical fluid to a supply channel; first and second back pressure regulators respectively provided in first and second channels branching from the supply channel; An analysis support device for supporting determination of analysis conditions for a supercritical fluid chromatograph including an analysis column provided upstream of the first back pressure regulator in the first flow path,
After instructing the supercritical fluid chromatograph to supply mobile phase to the feed channel at a flow rate of the mobile phase supplied to the first channel based on a predetermined total flow rate and sample introduction rate. , an analysis support apparatus comprising a detection unit that detects a first set value of the pressure of the first back pressure regulator at which the mobile phase is no longer supplied to the second flow path. 6. The analysis support apparatus according to claim 5, further comprising a setting unit that sets the pressure of said first back pressure regulator to said detected first set value. a storage unit that stores analysis conditions including a specified sample introduction ratio, a specified total flow rate of the mobile phase, and a specified second set value;
7. The analysis support apparatus according to claim 5, further comprising a determination updating unit that determines or updates the detected first set value as part of analysis conditions. The system according to any one of claims 5 to 7, further comprising a presentation unit that presents a warning when the set value of the pressure of the first back pressure regulator detected by the detection unit falls below a predetermined threshold value. The analysis support device according to any one of the items.

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