JP7103884B2 - Section preparation member - Google Patents

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JP7103884B2
JP7103884B2 JP2018142767A JP2018142767A JP7103884B2 JP 7103884 B2 JP7103884 B2 JP 7103884B2 JP 2018142767 A JP2018142767 A JP 2018142767A JP 2018142767 A JP2018142767 A JP 2018142767A JP 7103884 B2 JP7103884 B2 JP 7103884B2
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grid
section
liquid
liquid storage
knife
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JP2020020603A (en
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馨 山口
弘嗣 高瀬
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Nagoya City University
Shintec Co Ltd
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Nagoya City University
Shintec Co Ltd
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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B11/00—Servomotor systems without provision for follow-up action; Circuits therefor
    • F15B11/16—Servomotor systems without provision for follow-up action; Circuits therefor with two or more servomotors
    • F15B11/161—Servomotor systems without provision for follow-up action; Circuits therefor with two or more servomotors with sensing of servomotor demand or load
    • F15B11/165—Servomotor systems without provision for follow-up action; Circuits therefor with two or more servomotors with sensing of servomotor demand or load for adjusting the pump output or bypass in response to demand
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B21/00—Common features of fluid actuator systems; Fluid-pressure actuator systems or details thereof, not covered by any other group of this subclass
    • F15B21/08—Servomotor systems incorporating electrically operated control means
    • F15B21/087—Control strategy, e.g. with block diagram
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00—Measuring arrangements characterised by the use of optical techniques
    • G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
    • G01B11/026—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness by measuring distance between sensor and object
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B11/00—Measuring arrangements characterised by the use of optical techniques
    • G01B11/24—Measuring arrangements characterised by the use of optical techniques for measuring contours or curvatures
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/20—Fluid pressure source, e.g. accumulator or variable axial piston pump
    • F15B2211/205—Systems with pumps
    • F15B2211/2053—Type of pump
    • F15B2211/20546—Type of pump variable capacity
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/20—Fluid pressure source, e.g. accumulator or variable axial piston pump
    • F15B2211/205—Systems with pumps
    • F15B2211/2053—Type of pump
    • F15B2211/20546—Type of pump variable capacity
    • F15B2211/20553—Type of pump variable capacity with pilot circuit, e.g. for controlling a swash plate
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/30—Directional control
    • F15B2211/305—Directional control characterised by the type of valves
    • F15B2211/30525—Directional control valves, e.g. 4/3-directional control valve
    • F15B2211/3053—In combination with a pressure compensating valve
    • F15B2211/30535—In combination with a pressure compensating valve the pressure compensating valve is arranged between pressure source and directional control valve
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/30—Directional control
    • F15B2211/31—Directional control characterised by the positions of the valve element
    • F15B2211/3105—Neutral or centre positions
    • F15B2211/3111—Neutral or centre positions the pump port being closed in the centre position, e.g. so-called closed centre
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/30—Directional control
    • F15B2211/315—Directional control characterised by the connections of the valve or valves in the circuit
    • F15B2211/3157—Directional control characterised by the connections of the valve or valves in the circuit being connected to a pressure source, an output member and a return line
    • F15B2211/31576—Directional control characterised by the connections of the valve or valves in the circuit being connected to a pressure source, an output member and a return line having a single pressure source and a single output member
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/30—Directional control
    • F15B2211/35—Directional control combined with flow control
    • F15B2211/351—Flow control by regulating means in feed line, i.e. meter-in control
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/30—Directional control
    • F15B2211/35—Directional control combined with flow control
    • F15B2211/353—Flow control by regulating means in return line, i.e. meter-out control
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/50—Pressure control
    • F15B2211/51—Pressure control characterised by the positions of the valve element
    • F15B2211/513—Pressure control characterised by the positions of the valve element the positions being continuously variable, e.g. as realised by proportional valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/50—Pressure control
    • F15B2211/515—Pressure control characterised by the connections of the pressure control means in the circuit
    • F15B2211/5151—Pressure control characterised by the connections of the pressure control means in the circuit being connected to a pressure source and a directional control valve
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/60—Circuit components or control therefor
    • F15B2211/605—Load sensing circuits
    • F15B2211/6051—Load sensing circuits having valve means between output member and the load sensing circuit
    • F15B2211/6054—Load sensing circuits having valve means between output member and the load sensing circuit using shuttle valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/60—Circuit components or control therefor
    • F15B2211/63—Electronic controllers
    • F15B2211/6303—Electronic controllers using input signals
    • F15B2211/633—Electronic controllers using input signals representing a state of the prime mover, e.g. torque or rotational speed
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/60—Circuit components or control therefor
    • F15B2211/63—Electronic controllers
    • F15B2211/6303—Electronic controllers using input signals
    • F15B2211/634—Electronic controllers using input signals representing a state of a valve
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/60—Circuit components or control therefor
    • F15B2211/63—Electronic controllers
    • F15B2211/6303—Electronic controllers using input signals
    • F15B2211/6346—Electronic controllers using input signals representing a state of input means, e.g. joystick position
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/70—Output members, e.g. hydraulic motors or cylinders or control therefor
    • F15B2211/705—Output members, e.g. hydraulic motors or cylinders or control therefor characterised by the type of output members or actuators
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/70—Output members, e.g. hydraulic motors or cylinders or control therefor
    • F15B2211/705—Output members, e.g. hydraulic motors or cylinders or control therefor characterised by the type of output members or actuators
    • F15B2211/7051—Linear output members
    • F15B2211/7053—Double-acting output members
    • F15B2211/7054—Having equal piston areas
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F15—FLUID-PRESSURE ACTUATORS; HYDRAULICS OR PNEUMATICS IN GENERAL
    • F15B—SYSTEMS ACTING BY MEANS OF FLUIDS IN GENERAL; FLUID-PRESSURE ACTUATORS, e.g. SERVOMOTORS; DETAILS OF FLUID-PRESSURE SYSTEMS, NOT OTHERWISE PROVIDED FOR
    • F15B2211/00—Circuits for servomotor systems
    • F15B2211/70—Output members, e.g. hydraulic motors or cylinders or control therefor
    • F15B2211/71—Multiple output members, e.g. multiple hydraulic motors or cylinders
    • F15B2211/7135—Combinations of output members of different types, e.g. single-acting cylinders with rotary motors

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • General Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)

Description

本発明は、切片作製部材に関するものである。 The present invention relates to a section preparation member.

従来、電子顕微鏡等で観察する切片を試料から切削するナイフ部と、ナイフ部で切削された切片をストックするストック部と、ストック部に隣接して設けられたグリッド載物用の凹陥部と、を備える電子顕微鏡凍結切片作製用クライオ・ドライダイヤモンドナイフが知られている（例えば、特許文献１参照。）。
この電子顕微鏡凍結切片作製用クライオ・ドライダイヤモンドナイフでは、ナイフ部で切削された切片を、まつ毛プローブでストック部にストックする。その後、ストック部にストックされている切片を、まつげプローブによって凹陥部方向にずらし、凹陥部にセットされたグリッドの上面に移載する。次に、切片を載物したグリッドをピンセットで把持して凹陥部から取り出す。 Conventionally, a knife part for cutting a section to be observed with an electron microscope or the like from a sample, a stock part for stocking a section cut by the knife part, a recessed part for a grid loading provided adjacent to the stock part, and the like. A cryo-dry diamond knife for preparing an electron microscope frozen section is known (see, for example, Patent Document 1).
In this cryo-dry diamond knife for preparing frozen slices of an electron microscope, the slices cut by the knife portion are stocked in the stock portion by an eyelash probe. Then, the sections stocked in the stock portion are shifted in the recessed portion direction by the eyelash probe and transferred to the upper surface of the grid set in the recessed portion. Next, the grid on which the section is placed is grasped with tweezers and taken out from the recessed portion.

実用新案登録第３０１６９００号公報Utility Model Registration No. 3016900

しかしながら、特許文献１の電子顕微鏡凍結切片作製用クライオ・ドライダイヤモンドナイフでは、ナイフ部で切削された切片をグリッドの上面に載せるまでに、ナイフ部で切削された切片のストック部への移動、およびストック部から凹陥部にセットされたグリッドへの移載と、２段階の作業を経る必要がある。このため、切片をグリッド上に載せて取り出すまでに、手間が掛かるという問題がある。 However, in the cryo-dry diamond knife for preparing an electron microscope frozen section of Patent Document 1, the section cut by the knife part is moved to the stock part before being placed on the upper surface of the grid, and the section cut by the knife part is moved to the stock part. It is necessary to go through two steps of transfer from the stock part to the grid set in the recessed part. Therefore, there is a problem that it takes time and effort to put the sections on the grid and take them out.

さらに、切削された切片を、容器内に貯留された水の水面上に浮かべ、グリッドによって掬い取る場合もある。この場合、作業者は一方の手でグリッドを支持し、他方の手でまつげプローブによって切片をたぐり寄せる必要があり、狭い領域での両手の作業は困難であるとともに、水面張力等の作用によって、水面上に浮かんだ切片をグリッドに載せるのが困難な場合もある。 Furthermore, the cut section may be floated on the surface of the water stored in the container and scooped up by a grid. In this case, the operator needs to support the grid with one hand and pull the section with the eyelash probe with the other hand, which makes it difficult to work with both hands in a narrow area and due to the action of water surface tension and the like. It can be difficult to place sections floating on the surface of the water on the grid.

本発明は上述した事情に鑑みてなされたものであって、切片をグリッド上に容易かつ確実に載せて、観察用試料の作製を効率良く行うことができる切片作製部材を提供することを目的としている。 The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a section preparation member capable of efficiently placing a section on a grid and efficiently producing an observation sample. There is.

上記目的を達成するため、本発明は以下の手段を提供する。
本発明の一態様は、容器本体と、前記容器本体における第１水平方向の一端部に設けられ、試料から切片を切り出すナイフ部と、前記ナイフ部で切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、前記切片を載置する載置面を有した板状のグリッドが、前記載置面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、前記第１水平方向と直交する第２水平方向を前記容器本体の幅方向としたとき、前記グリッド収容部が、前記容器本体の幅方向両側にそれぞれ設けられている切片作製部材を提供する。 In order to achieve the above object, the present invention provides the following means.
In one aspect of the present invention, a container body, a knife portion provided at one end in the first horizontal direction of the container body, and a knife portion for cutting a section from a sample, and a liquid on which the section cut out by the knife portion floats are stored. A plate-shaped grid having a liquid storage portion and a mounting surface on which the section is placed is a grid storage portion in which the above-mentioned mounting surface is inclined and accommodated with the above-mentioned mounting surface facing diagonally upward. The grid accommodating portion is provided with a liquid storage portion and a grid accommodating portion communicating with each other in a region including the liquid level of the liquid, and when the second horizontal direction orthogonal to the first horizontal direction is defined as the width direction of the container body. Provides section making members provided on both sides of the container body in the width direction .

本態様によれば、液体貯留部とグリッド収容部とは、液体の液面を含む領域で連通する。ナイフ部によって試料から切り出された切片は、液体貯留部に貯留された液体に浮かぶ。液体に浮かんだ切片はグリッド収容部に移動され、グリッド収容部に収容されたグリッドの載置面に載せられる。グリッドは、載置面を斜め上方に向けた状態で傾斜して収容されているので、液体の液面に浮かぶ切片を、斜め上方を向く載置面に載せればよい。このようにして、切片をグリッド上に容易かつ確実に載せることが可能となる。例えば、本態様を用いると、容器本体内のスペースにおいて、グリッド収容部を幅方向両側に配置することによってナイフ部に近接した側にグリッド収容部を配置でき、これにより、限られた視野である顕微鏡の視野の範囲内にグリッド収容部が収まりやすくなり、これは観察用試料の作製作業の容易化に寄与できる。 According to this aspect, the liquid storage part and the grid storage part communicate with each other in the region including the liquid level of the liquid . The section cut out from the sample by the knife part floats on the liquid stored in the liquid storage part. The slices floating in the liquid are moved to the grid housing and placed on the mounting surface of the grid housed in the grid housing. Since the grid is housed in an inclined manner with the mounting surface facing diagonally upward, the section floating on the liquid surface of the liquid may be placed on the mounting surface facing diagonally upward. In this way, the sections can be easily and reliably placed on the grid. For example, according to this aspect, in the space inside the container body, the grid accommodating portion can be arranged on the side close to the knife portion by arranging the grid accommodating portions on both sides in the width direction, whereby the field of view is limited. The grid housing can be easily accommodated within the field of view of the microscope, which can contribute to facilitating the preparation work of the observation sample.

上記態様においては、前記グリッド収容部が、前記グリッドの一部を前記液体貯留部に貯留される前記液体の液面よりも上方に突出させた状態で前記グリッドを収容していてもよい。
このようにすることで、切片をグリッドの載置面に載せる際、液面から上方に突出したグリッドの一部をピンセット等で容易に保持することができる。したがって、切片をグリッド上に載せる作業を容易に行うことが可能となる。 In the above aspect, the grid accommodating portion may accommodate the grid in a state in which a part of the grid is projected upward from the liquid surface of the liquid stored in the liquid storage portion.
By doing so, when the section is placed on the mounting surface of the grid, a part of the grid protruding upward from the liquid surface can be easily held by tweezers or the like. Therefore, the work of placing the sections on the grid can be easily performed.

本発明の他の態様は、容器本体と、試料から切片を切り出すナイフ部と、前記ナイフ部で切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、前記切片を載置する板状のグリッドが、その厚さ方向の一方の面である第１の面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、前記グリッド収容部が、前記グリッドの一部を前記液体貯留部に貯留される前記液体の前記液面よりも上方に突出させた状態で前記グリッドを収容するように構成され、前記グリッド収容部が、前記グリッドを、前記第１の面の下側が第１の壁部に突き当てられ、前記グリッドの前記厚さ方向の他方の面である第２の面の上側が第２の壁部によって支持されることによって、前記第１の面が前記ナイフ部側に向かって漸次下方に傾斜した状態で収容するように構成されている、切片作製部材である。
本態様では、上記のように、グリッドの下端を第１の壁部に突き当てることで、グリッドをその上端から下端に向かってナイフ部側に向かって漸次下方に傾斜するように配置できる。このようにグリッドを配置し、切片をグリッドに近づけていくと、切片の移動方向前方の端部がグリッドの載置面に接触する。その後、グリッドを載置面に沿った方向に斜めに引き上げていけば、切片をグリッド上に容易かつ確実に載せることができる。 Another aspect of the present invention is a container body, a knife portion for cutting a section from a sample, a liquid storage portion for storing a liquid in which the section cut out by the knife portion floats, and a plate shape on which the section is placed. The grid is a grid accommodating portion in which the first surface, which is one surface in the thickness direction thereof, is inclined and accommodated in a state of being obliquely upward, and the liquid storage portion and the liquid level of the liquid are accommodated. The grid is provided with a grid accommodating portion communicating with the including region, and the grid accommodating portion projects a part of the grid upward from the liquid level of the liquid stored in the liquid storage portion. The grid accommodating portion abuts the grid against the first wall portion on the lower side of the first surface, and is the other surface of the grid in the thickness direction. A section preparation member configured such that the upper side of the second surface is supported by the second wall portion so that the first surface is accommodated in a state of being gradually inclined downward toward the knife portion side. Is.
In this embodiment, as described above, by abutting the lower end of the grid against the first wall portion, the grid can be arranged so as to gradually incline downward from the upper end toward the lower end toward the knife portion. When the grid is arranged in this way and the sections are brought closer to the grid, the front end of the section in the moving direction comes into contact with the mounting surface of the grid. After that, if the grid is pulled up diagonally along the mounting surface, the sections can be easily and reliably placed on the grid.

上記態様においては、前記液体貯留部および前記グリッド収容部を囲う周壁部と、前記周壁部の内側で、前記液体貯留部と前記グリッド収容部とを区画し、前記周壁部よりも低く形成された区画壁とを備え、前記区画壁の上方が前記液面を含む領域とされていてもよい。
このようにすることで、周壁部の内側に液体を入れ、その液面レベルを区画壁よりも上方となるようにすれば、液体貯留部とグリッド収容部の間で液体が連通する。これにより、液体貯留部とグリッド収容部との間で切片を液体に浮かべたまま、容易に移動させることができる。 In the above aspect, the peripheral wall portion surrounding the liquid storage portion and the grid accommodating portion, and the liquid storage portion and the grid accommodating portion are partitioned inside the peripheral wall portion, and the liquid storage portion and the grid accommodating portion are formed lower than the peripheral wall portion. A partition wall may be provided, and the area above the partition wall may be a region including the liquid level .
By doing so, if the liquid is put inside the peripheral wall portion and the liquid level is set above the partition wall, the liquid communicates between the liquid storage portion and the grid accommodating portion. This allows the sections to be easily moved between the liquid reservoir and the grid containment while the sections are floating in the liquid.

本発明のさらに他の態様は、水平方向において一端部および他端部を有する容器本体と、前記容器本体における前記一端部に設けられ、試料から切片を切り出すナイフ部と、前記容器本体内に設けられ、前記ナイフ部によって切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、前記切片を載置する載置面を有した板状のグリッドが、前記載置面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、前記グリッド収容部が、前記液体貯留部の前記水平方向の中央よりも前記一端部に近い側に設けられている、切片作製部材である。
このようにすることで、ナイフ部によって試料から切り出された切片を、グリッド収容部に収容されたグリッドまで移動させる際、切片の移動量が少なくて済む。また、ナイフ部で切片の切り出し作業を行う際に用いる顕微鏡の視野の範囲内に、グリッド収容部までが収まりやすくなる。したがって、観察用試料の作製作業を容易に行うことが可能となる。 Yet another aspect of the present invention is a container body having one end and an other end in the horizontal direction, a knife portion provided at the one end of the container body to cut out a section from a sample, and a knife portion provided inside the container body. A plate-shaped grid having a liquid storage portion for storing the liquid in which the section is floated and a mounting surface on which the section is placed, which is cut out by the knife portion, has the above-mentioned mounting surface facing diagonally upward. A grid accommodating portion that is tilted and accommodated in a vertical state, and includes the liquid accommodating portion and a grid accommodating portion that communicates with each other in a region including the liquid level of the liquid, and the grid accommodating portion is the liquid storage unit. It is a section making member provided on the side closer to the one end portion than the center in the horizontal direction of the above.
By doing so, when the section cut out from the sample by the knife portion is moved to the grid accommodated in the grid accommodating portion, the amount of movement of the section can be small. In addition, the grid accommodating portion can be easily accommodated within the field of view of the microscope used when cutting out the section with the knife portion. Therefore, it is possible to easily perform the work of preparing the observation sample .

本発明によれば、切片をグリッド上に容易かつ確実に載せて、観察用試料の作製を効率良く行うことができるという効果を奏する。 According to the present invention, there is an effect that the sections can be easily and surely placed on the grid to efficiently prepare an observation sample.

本発明の一実施形態に係る切片作製部材の全体構成を示す斜視図である。It is a perspective view which shows the whole structure of the section making member which concerns on one Embodiment of this invention. 図１の切片作製部材の平面図である。It is a top view of the section making member of FIG. 図１の切片作製部材の液体貯留部の底面形状を示す側面図である。It is a side view which shows the bottom surface shape of the liquid storage part of the section making member of FIG. 図１の切片作製部材のグリッド収容部を示す斜視図である。It is a perspective view which shows the grid accommodating part of the section making member of FIG. 図１の切片作製部材のグリッド収容部を示す平面図である。It is a top view which shows the grid accommodating part of the section making member of FIG. 本発明の一実施形態に係る切片作製部材のグリッド収容部を示す断面図である。It is sectional drawing which shows the grid accommodating part of the section making member which concerns on one Embodiment of this invention. 図６切片作製部材の変形例を示す断面図である。FIG. 6 is a cross-sectional view showing a modified example of the section making member. 本発明の一実施形態に係る切片作製部材のグリッド収容部の変形例を示す断面図である。It is sectional drawing which shows the modification of the grid accommodating part of the section making member which concerns on one Embodiment of this invention.

本発明の一実施形態に係る切片作製部材１について、図面を参照して以下に説明する。
本実施形態に係る切片作製部材１は、図１および図２に示されるように、容器本体１０と、容器本体１０に形成されたナイフ部２と、液体貯留部３と、グリッド収容部４と、連通部５とを備えている。 The section preparation member 1 according to the embodiment of the present invention will be described below with reference to the drawings.
As shown in FIGS. 1 and 2, the section preparation member 1 according to the present embodiment includes a container main body 10, a knife portion 2 formed in the container main body 10, a liquid storage portion 3, and a grid accommodating portion 4. , The communication unit 5 is provided.

容器本体１０は、平面視長方形状で、底部１１と、底部１１の外周部から底部１１に直交して上方に立ち上がる周壁部１２とを一体的に備えている。容器本体１０の下部１０ｄは、切片切出し装置（図示無し）に設けられた溝部（図示無し）に嵌め込まれる。 The container body 10 has a rectangular shape in a plan view, and integrally includes a bottom portion 11 and a peripheral wall portion 12 that rises upward from the outer peripheral portion of the bottom portion 11 at right angles to the bottom portion 11. The lower portion 10d of the container body 10 is fitted into a groove (not shown) provided in a section cutting device (not shown).

周壁部１２の上端１２ｔは、周壁部１２の全周にわたって、底部１１から一定の高さに形成されている。周壁部１２の内側は、上方に向かって開口している。
図１および図３に示されるように、周壁部１２の内側には、平面視長方形状の容器本体１０において長辺方向一方の端部１０ａから他方の端部１０ｂ側に向かって漸次下方に傾斜する傾斜面１３が形成されている。 The upper end 12t of the peripheral wall portion 12 is formed at a constant height from the bottom portion 11 over the entire circumference of the peripheral wall portion 12. The inside of the peripheral wall portion 12 is open upward.
As shown in FIGS. 1 and 3, the inside of the peripheral wall portion 12 is gradually inclined downward from one end 10a in the long side direction toward the other end 10b in the rectangular container body 10 in a plan view. An inclined surface 13 is formed.

ナイフ部２は、容器本体１０の端部１０ａに設けられている。ナイフ部２は、傾斜面１３の上端部に設けられている。ナイフ部２は、刃先角度が３５°から４５°の範囲であることが好ましい。本実施形態においては、一例として、容器本体１０の端部１０ａにおいて、周壁部１２の外周面１２ｆと、傾斜面１３とに沿う開き角を有しているナイフ部２を用いて説明する。ナイフ部２は、切片切出し装置に保持された試料に対し、容器本体１０を上方に相対移動させることで、試料から所定の厚さの切片１００を切り出す。
なお、切片１００を切り出す試料は、生体、樹脂、金属または電子基板等そのものであってもよいし、生体、樹脂、金属または電子基板等を樹脂等に埋設したものであってもよい。 The knife portion 2 is provided at the end portion 10a of the container body 10. The knife portion 2 is provided at the upper end portion of the inclined surface 13. The knife portion 2 preferably has a cutting edge angle in the range of 35 ° to 45 °. In the present embodiment, as an example, a knife portion 2 having an opening angle along the outer peripheral surface 12f of the peripheral wall portion 12 and the inclined surface 13 at the end portion 10a of the container main body 10 will be described. The knife portion 2 cuts out a section 100 having a predetermined thickness from the sample by moving the container body 10 upward relative to the sample held in the section cutting device.
The sample from which the section 100 is cut out may be a living body, a resin, a metal, an electronic substrate, or the like itself, or a living body, a resin, a metal, an electronic substrate, or the like embedded in a resin or the like.

液体貯留部３は、容器本体１０において周壁部１２の内側に形成されている。液体貯留部３は、ナイフ部２が設けられた容器本体１０の一方の端部１０ａ側が、端部１０ａと端部１０ｂとを結ぶ方向に直交する方向の幅寸法が小さい狭幅部３Ａとされ、他方の端部１０ｂ側が、狭幅部３Ａよりも幅寸法の大きい広幅部３Ｂとされている。 The liquid storage portion 3 is formed inside the peripheral wall portion 12 in the container body 10. The liquid storage portion 3 is a narrow portion 3A in which one end 10a side of the container body 10 provided with the knife portion 2 has a small width dimension in a direction orthogonal to the direction connecting the end portion 10a and the end portion 10b. The other end portion 10b side is a wide portion 3B having a width dimension larger than that of the narrow width portion 3A.

液体貯留部３の狭幅部３Ａの底面は、傾斜面１３によって形成されている。液体貯留部３の広幅部３Ｂの底面は、傾斜面１３の下端と、容器本体１０の端部１０ｂとの間で下方に湾曲した湾曲面１４により形成されている。 The bottom surface of the narrow portion 3A of the liquid storage portion 3 is formed by the inclined surface 13. The bottom surface of the wide portion 3B of the liquid storage portion 3 is formed by a curved surface 14 that is curved downward between the lower end of the inclined surface 13 and the end portion 10b of the container body 10.

液体貯留部３内には、純水等、不純物のない水（液体：図６参照）Ｗが貯留される。水Ｗは、例えば、周壁部１２の上端１２ｔレベルまで満たされる。液体貯留部３内の水Ｗには、ナイフ部２で切り出された切片１００が浮かぶようになっている。 Water without impurities such as pure water (liquid: see FIG. 6) W is stored in the liquid storage unit 3. The water W is filled up to, for example, the upper end 12t level of the peripheral wall portion 12. The section 100 cut out by the knife portion 2 floats on the water W in the liquid storage portion 3.

グリッド収容部４は、図４に示されるように、切片１００を載置するグリッド２００を収容する。
グリッド２００は、例えば円板状で、その一面側に切片１００を載置する載置面２００ｆを有している。グリッド２００には、その両面を貫通する貫通孔２００ｈを形成してもよい。また、グリッド２００の表面に、支持膜（コロジオンまたはカーボン等）を施してもよい。グリッド２００は、板状ではなく、メッシュ状に形成してもよい。 The grid accommodating section 4 accommodates the grid 200 on which the section 100 is placed, as shown in FIG.
The grid 200 has, for example, a disk shape, and has a mounting surface 200f on which the section 100 is mounted on one surface side thereof. The grid 200 may be formed with through holes 200h penetrating both sides thereof. Further, a support film (collodion, carbon, etc.) may be applied to the surface of the grid 200. The grid 200 may be formed in a mesh shape instead of a plate shape.

グリッド収容部４は、図１および図２に示されるように、容器本体１０において、ナイフ部２が設けられた一方の端部１０ａ側に設けられている。グリッド収容部４は、液体貯留部３の狭幅部３Ａの幅方向両側にそれぞれ設けられている。すなわち、グリッド収容部４は、液体貯留部３において、ナイフ部２に近接した端部１０ａ側（狭幅部３Ａ側）に設けられている。 As shown in FIGS. 1 and 2, the grid accommodating portion 4 is provided in the container main body 10 on the one end portion 10a side where the knife portion 2 is provided. The grid accommodating portion 4 is provided on both sides of the narrow width portion 3A of the liquid storage portion 3 in the width direction. That is, the grid accommodating portion 4 is provided in the liquid storage portion 3 on the end portion 10a side (narrow width portion 3A side) close to the knife portion 2.

各グリッド収容部４は、グリッド２００を収容する収容凹部４１を備えている。本実施形態において、各グリッド収容部４は、図５に示されるように、容器本体１０の端部１０ａと端部１０ｂとを結ぶ長手方向に２つの収容凹部４１が並んで設けられている。 Each grid accommodating portion 4 includes an accommodating recess 41 accommodating the grid 200. In the present embodiment, as shown in FIG. 5, each grid accommodating portion 4 is provided with two accommodating recesses 41 arranged side by side in the longitudinal direction connecting the end 10a and the end 10b of the container body 10.

グリッド収容部４には、図４に示されるように、周壁部１２の上端１２ｔよりも所定寸法低い位置に、水平面４２が形成されている。水平面４２は、液体貯留部３に貯留される水Ｗの液面レベルＷＬよりも低い位置に設けられている。各収容凹部４１は、水平面４２から下方に窪むように形成されている。 As shown in FIG. 4, the grid accommodating portion 4 is formed with a horizontal plane 42 at a position lower than the upper end 12t of the peripheral wall portion 12 by a predetermined dimension. The horizontal plane 42 is provided at a position lower than the liquid level WL of the water W stored in the liquid storage unit 3. Each accommodating recess 41 is formed so as to be recessed downward from the horizontal plane 42.

各収容凹部４１は、例えば、容器本体１０の端部１０ａと端部１０ｂとを結ぶ長手方向に長い平面視長円形状とされている。各収容凹部４１において、容器本体１０の長手方向に直交する短手方向の一方側の壁部４１ａと、他方側の壁部４１ｂとは、間隔をあけて互いに平行に設けられている。収容凹部４１の一方側の壁部４１ａは、周壁部１２により形成されている。収容凹部４１の他方側の壁部４１ｂは、後述する区画壁１５により形成されている。 Each storage recess 41 has, for example, an oval shape in a plan view that is long in the longitudinal direction connecting the end 10a and the end 10b of the container body 10. In each storage recess 41, the wall portion 41a on one side in the lateral direction orthogonal to the longitudinal direction of the container body 10 and the wall portion 41b on the other side are provided in parallel with each other at intervals. The wall portion 41a on one side of the accommodating recess 41 is formed by the peripheral wall portion 12. The wall portion 41b on the other side of the accommodating recess 41 is formed by a partition wall 15 described later.

連通部５は、液体貯留部３の狭幅部３Ａと、その両側にそれぞれ設けられたグリッド収容部４との間に形成されている。連通部５は、液体貯留部３の狭幅部３Ａとグリッド収容部４の間に設けられた区画壁１５によって形成されている。区画壁１５は、周壁部１２の内側において、液体貯留部３の狭幅部３Ａとグリッド収容部４の各収容凹部４１とを区画する。
区画壁１５は、周壁部１２の上端１２ｔよりも低く形成されている。本実施形態において、区画壁１５の上端は、水平面４２に連続している。区画壁１５は、液体貯留部３に貯留される水Ｗの液面レベルＷＬよりも下方に設けられている。これにより、区画壁１５の上方において、液体貯留部３に貯留される水Ｗは、液面レベルＷＬを含む液面近くの領域において、収容凹部４１に連通する。すなわち、区画壁１５の上方が、連通部５である。 The communication portion 5 is formed between the narrow width portion 3A of the liquid storage portion 3 and the grid accommodating portions 4 provided on both sides thereof. The communication portion 5 is formed by a partition wall 15 provided between the narrow width portion 3A of the liquid storage portion 3 and the grid accommodating portion 4. The partition wall 15 partitions the narrow portion 3A of the liquid storage portion 3 and the respective storage recesses 41 of the grid storage portion 4 inside the peripheral wall portion 12.
The partition wall 15 is formed lower than the upper end 12t of the peripheral wall portion 12. In the present embodiment, the upper end of the partition wall 15 is continuous with the horizontal plane 42. The partition wall 15 is provided below the liquid level WL of the water W stored in the liquid storage unit 3. As a result, the water W stored in the liquid storage unit 3 above the partition wall 15 communicates with the storage recess 41 in the region near the liquid level including the liquid level WL. That is, the communication portion 5 is above the partition wall 15.

このような切片作製部材１において、板状のグリッド２００は、図４に示されるように、グリッド収容部４の収容凹部４１に、切片１００が載置される載置面２００ｆを斜め上方に向けた状態で傾斜して収容される。具体的には、グリッド２００は、その下端２００ａを、収容凹部４１の壁部４１ｂ（区画壁１５）と底面４１ｃとの角部に突き当てた状態で、上端２００ｂ側を、壁部４１ａ（周壁部１２）に立て掛けるようにして収容される。このように、グリッド２００は、下端２００ａを液体貯留部３との区画壁１５である壁部４１ｂ側に突き当てることで、上端２００ｂから下端２００ａに向かって、狭幅部３Ａに設けられたナイフ部２側に漸次下方に傾斜している。 In such a section preparation member 1, as shown in FIG. 4, the plate-shaped grid 200 faces the mounting surface 200f on which the section 100 is mounted obliquely upward in the storage recess 41 of the grid storage portion 4. It is housed in an inclined state. Specifically, in the grid 200, the lower end 200a is abutted against the corner portion between the wall portion 41b (partition wall 15) and the bottom surface 41c of the accommodating recess 41, and the upper end 200b side is the wall portion 41a (peripheral wall). It is housed so as to lean against the part 12). As described above, the grid 200 is provided with a knife provided in the narrow portion 3A from the upper end 200b toward the lower end 200a by abutting the lower end 200a against the wall portion 41b, which is the partition wall 15 with the liquid storage portion 3. It is gradually inclined downward toward the portion 2.

このとき、グリッド２００は、水平面を基準とした傾斜角度θが、４０°以上７０°以下となるように収容凹部４１に収容されるのが好ましい。収容凹部４１に収容されるグリッド２００のさらに好ましい傾斜角度θは、４５°以上６０°以下である。これにより、グリッド２００の載置面２００ｆに、水Ｗに浮かべた切片１００を載置させやすくなる。 At this time, the grid 200 is preferably accommodated in the accommodating recess 41 so that the inclination angle θ with respect to the horizontal plane is 40 ° or more and 70 ° or less. A more preferable inclination angle θ of the grid 200 accommodated in the accommodating recess 41 is 45 ° or more and 60 ° or less. As a result, the section 100 floated on the water W can be easily placed on the mounting surface 200f of the grid 200.

また、グリッド２００は、グリッド収容部４の収容凹部４１に、上端２００ｂ側の少なくとも一部を、水Ｗの液面レベルＷＬよりも上方に突出した状態で収容される。グリッド２００の液面レベルＷＬから上方への突出寸法は、例えば、グリッド２００の直径が３ｍｍである場合、０．１ｍｍから０．５ｍｍ程度とするのが好ましい。 Further, the grid 200 is accommodated in the accommodating recess 41 of the grid accommodating portion 4 in a state where at least a part of the upper end 200b side is projected above the liquid level WL of the water W. The upward protrusion dimension of the grid 200 from the liquid level WL is preferably about 0.1 mm to 0.5 mm, for example, when the diameter of the grid 200 is 3 mm.

このように構成された本実施形態に係る切片作製部材１を用いて切片１００を形成するには、切片作製部材１を、切片１００を切り出す試料を保持した切片切出し装置に対向してセットする。このとき、グリッド収容部４の収容凹部４１に、グリッド２００を収容しておく。 In order to form the section 100 using the section preparation member 1 according to the present embodiment configured as described above, the section preparation member 1 is set facing the section cutting device holding the sample for cutting the section 100. At this time, the grid 200 is accommodated in the accommodating recess 41 of the grid accommodating portion 4.

その後、切片切出し装置に保持された試料に対し、容器本体１０に設けられたナイフ部２を上方に相対移動させる。これにより、試料から所定の厚さの切片１００が切り出される。図４に示されるように、切片１００は、ナイフ部２から液体貯留部３の狭幅部３Ａに貯留された水Ｗ上に浮かぶ。 After that, the knife portion 2 provided in the container body 10 is relatively moved upward with respect to the sample held in the intercept cutting device. As a result, a section 100 having a predetermined thickness is cut out from the sample. As shown in FIG. 4, the section 100 floats on the water W stored in the narrow portion 3A of the liquid storage portion 3 from the knife portion 2.

作業者は、まつ毛プローブ等を用い、水Ｗ上に浮かんだ切片１００を、液体貯留部３から連通部５を経て、グリッド収容部４に移動させていく。さらに、切片１００を、グリッド収容部４の収容凹部４１に収容されたグリッド２００の載置面２００ｆに寄せる。グリッド２００は傾斜しているので、切片１００において、移動方向前方の端部１００ｆがグリッド２００の載置面２００ｆに接触した状態で、グリッド２００をピンセット等で保持して上方に引き上げると、載置面２００ｆに切片１００が順次付着する。 The operator uses an eyelash probe or the like to move the section 100 floating on the water W from the liquid storage unit 3 to the grid storage unit 4 via the communication unit 5. Further, the section 100 is brought close to the mounting surface 200f of the grid 200 accommodated in the accommodating recess 41 of the grid accommodating portion 4. Since the grid 200 is inclined, in the section 100, when the grid 200 is held by tweezers or the like and pulled upward while the front end 100f in the moving direction is in contact with the mounting surface 200f of the grid 200, the grid 200 is placed. Section 100 is sequentially attached to the surface 200f.

このようにして、切片１００がグリッド２００の載置面２００ｆに載置された後、グリッド２００を切片作製部材１から取り出す。このとき、複数枚の切片１００を、一つのグリッド２００の載置面２００ｆに載置して取り出してもよい。
その後、グリッド２００に載置されて取り出された切片１００は、観察用試料として試料ホルダー等に移載され、電子顕微鏡等で観察される。 In this way, after the section 100 is placed on the mounting surface 200f of the grid 200, the grid 200 is taken out from the section preparation member 1. At this time, a plurality of sections 100 may be placed on the mounting surface 200f of one grid 200 and taken out.
After that, the section 100 placed on the grid 200 and taken out is transferred to a sample holder or the like as an observation sample and observed with an electron microscope or the like.

このように、本実施形態に係る切片作製部材１によれば、液体貯留部３とグリッド収容部４とが、連通部５を通して連通する。これにより、ナイフ部２によって試料から切り出された切片１００は、水Ｗに浮かべたまま、連通部５を通してグリッド収容部４に移動させ、グリッド２００の載置面２００ｆに載せることができる。しかも、グリッド収容部４が、載置面２００ｆを斜め上方に向けた状態でグリッド２００を傾斜させて収容している。これにより、水Ｗの液面に浮かぶ切片１００を、斜め上方を向く載置面２００ｆに容易かつ確実に載せることができる。したがって、切片１００をグリッド２００上に容易かつ確実に載せて、観察用試料の作製を効率良く行うことができる。 As described above, according to the section preparation member 1 according to the present embodiment, the liquid storage unit 3 and the grid storage unit 4 communicate with each other through the communication unit 5. As a result, the section 100 cut out from the sample by the knife portion 2 can be moved to the grid accommodating portion 4 through the communication portion 5 while floating on the water W, and can be placed on the mounting surface 200f of the grid 200. Moreover, the grid accommodating portion 4 accommodates the grid 200 in an inclined manner with the mounting surface 200f facing diagonally upward. Thereby, the section 100 floating on the liquid surface of the water W can be easily and surely placed on the mounting surface 200f facing diagonally upward. Therefore, the section 100 can be easily and surely placed on the grid 200 to efficiently prepare an observation sample.

また、グリッド２００が、その一部を液面レベルＷＬよりも上方に突出させた状態でグリッド収容部４に収容されている。このようにすることで、切片１００をグリッド２００の載置面２００ｆに載せる際、液面から上方に突出したグリッド２００の一部をピンセット等で容易に保持することができる。したがって、切片１００をグリッド２００上に載せて取り出す作業を容易に行うことが可能となる。 Further, the grid 200 is housed in the grid accommodating portion 4 with a part thereof protruding above the liquid level WL. By doing so, when the section 100 is placed on the mounting surface 200f of the grid 200, a part of the grid 200 protruding upward from the liquid surface can be easily held by tweezers or the like. Therefore, it is possible to easily carry out the work of placing the section 100 on the grid 200 and taking it out.

また、グリッド収容部４が、グリッド２００を、ナイフ部２側に向かって漸次下方に傾斜した状態で収容している。このようにすることで、グリッド２００の載置面２００ｆが、ナイフ部２から切り出された切片１００側（切片１００が移動されてくる側）を向くことになる。これにより、グリッド２００に近づけていけば、切片１００をグリッド２００に容易かつ確実に載せることができる。 Further, the grid accommodating portion 4 accommodates the grid 200 in a state of being gradually inclined downward toward the knife portion 2 side. By doing so, the mounting surface 200f of the grid 200 faces the section 100 side (the side to which the section 100 is moved) cut out from the knife portion 2. As a result, the section 100 can be easily and surely placed on the grid 200 as it approaches the grid 200.

また、液体貯留部３とグリッド収容部４とを区画する区画壁１５の上方が連通部５とされている。このようにすることで、周壁部１２の内側に水Ｗを入れ、その液面レベルＷＬを区画壁１５よりも上方となるようにすれば、液体貯留部３とグリッド収容部４との間で水Ｗが連通する。これにより、液体貯留部３とグリッド収容部４との間で切片１００を水Ｗに浮かべたまま、容易に移動させることができる。 Further, the communication portion 5 is above the partition wall 15 that partitions the liquid storage portion 3 and the grid accommodating portion 4. By doing so, if water W is put inside the peripheral wall portion 12 and the liquid level WL thereof is set above the partition wall 15, the liquid storage portion 3 and the grid accommodating portion 4 can be separated from each other. Water W communicates. As a result, the section 100 can be easily moved between the liquid storage unit 3 and the grid storage unit 4 while floating on the water W.

また、グリッド収容部４が、液体貯留部３においてナイフ部２に近接した側に設けられている。このようにすることで、ナイフ部２によって試料から切り出された切片１００を、グリッド収容部４に収容されたグリッド２００まで移動させる際、切片１００の移動量が少なくて済む。また、ナイフ部２で切片１００の切り出し作業を行う際に用いる顕微鏡の視野の範囲内に、グリッド収容部４までが収まりやすくなる。したがって、切片１００の作製作業を容易に行うことが可能となる。 Further, the grid accommodating portion 4 is provided on the side of the liquid storage portion 3 close to the knife portion 2. By doing so, when the section 100 cut out from the sample by the knife portion 2 is moved to the grid 200 housed in the grid accommodating portion 4, the amount of movement of the section 100 can be small. Further, the grid accommodating portion 4 can be easily accommodated within the field of view of the microscope used when cutting out the section 100 with the knife portion 2. Therefore, the operation of preparing the section 100 can be easily performed.

なお、上記実施形態においては、グリッド収容部４を、液体貯留部３の狭幅部３Ａの両側に設け、それぞれ２つの収容凹部４１を並設するようにしたが、その設置数、配置等については適宜変更することができる。
例えば、図６および図７に示すように、グリッド収容部４Ｂは、液体貯留部３の狭幅部３Ａに対し、容器本体１０の端部１０ａと端部１０ｂとを結ぶ長手方向に２つの収容凹部４１を並べるとともに、容器本体１０の短手方向に前後して収容凹部４１を２列に設けるようにしてもよい。容器本体１０の短手方向に前後する収容凹部４１間には、収容凹部間連通部２５を形成する。液体貯留部３に貯留される水Ｗを、連通部５および収容凹部間連通部２５を介して、複数の収容凹部４１に連通させる。これにより、切片１００を、水Ｗに浮かべたまま移動し、各収容凹部４１に収容されるグリッド２００に載置させることができる。 In the above embodiment, the grid accommodating portions 4 are provided on both sides of the narrow width portion 3A of the liquid storage portion 3, and two accommodating recesses 41 are arranged side by side. Can be changed as appropriate.
For example, as shown in FIGS. 6 and 7, the grid accommodating portion 4B accommodates the narrow width portion 3A of the liquid storage portion 3 in the longitudinal direction connecting the end portion 10a and the end portion 10b of the container body 10. In addition to arranging the recesses 41, the storage recesses 41 may be provided in two rows in the front-rear direction in the lateral direction of the container body 10. A communication portion 25 between the accommodating recesses is formed between the accommodating recesses 41 that are moved back and forth in the lateral direction of the container body 10. The water W stored in the liquid storage portion 3 is communicated with the plurality of storage recesses 41 via the communication portion 5 and the communication portion 25 between the storage recesses. As a result, the section 100 can be moved while floating on the water W and placed on the grid 200 accommodated in each accommodating recess 41.

また、例えば、図８に示されるように、グリッド収容部４Ｃの収容凹部４１Ｃは、所定の角度で傾斜した傾斜面４５を備えていてもよい。グリッド２００は、載置面２００ｆを斜め上方に向けた状態で、傾斜面４５に沿って設けられ、その下部側が、水Ｗの液面レベルＷＬよりも下方に沈み、上部側が液面レベルＷＬよりも上方に露出する。
このような構成においても、水Ｗに浮かべた切片１００を、連通部５を通してグリッド収容部４のグリッド２００の載置面２００ｆに載置することができる。 Further, for example, as shown in FIG. 8, the accommodating recess 41C of the grid accommodating portion 4C may include an inclined surface 45 inclined at a predetermined angle. The grid 200 is provided along the inclined surface 45 with the mounting surface 200f facing diagonally upward, the lower side thereof sinks below the liquid level WL of the water W, and the upper side thereof is below the liquid level WL. Is also exposed upwards.
Even in such a configuration, the section 100 floated on the water W can be placed on the mounting surface 200f of the grid 200 of the grid accommodating portion 4 through the communication portion 5.

また、上記実施形態においては、液体貯留部３の広幅部３Ｂの底面が、下方に湾曲した湾曲面１４により形成されているものを例示したが、これに代えて、平面等の湾曲していないものを採用してもよい。
また、上記実施形態においては、液体貯留部３内に貯留される液体として、不純物のない水Ｗを採用したが、これに代えて、水にエタノール等を少量添加して表面張力を調整したもの等、他の液体を採用してもよい。 Further, in the above embodiment, the bottom surface of the wide portion 3B of the liquid storage portion 3 is formed by the curved surface 14 curved downward, but instead of this, the flat surface or the like is not curved. You may adopt the thing.
Further, in the above embodiment, water W without impurities is adopted as the liquid stored in the liquid storage unit 3, but instead of this, a small amount of ethanol or the like is added to water to adjust the surface tension. Etc., other liquids may be adopted.

１ 切片作製部材
２ ナイフ部
３ 液体貯留部
４，４Ｂ，４Ｃ グリッド収容部
５ 連通部
１２ 周壁部
１５ 区画壁
１００ 切片
２００ グリッド
２００ｆ 載置面
Ｗ 水（液体）
ＷＬ 液面レベル 1 Section preparation member 2 Knife part 3 Liquid storage part 4, 4B, 4C Grid storage part 5 Communication part 12 Peripheral wall part 15 Section wall 100 Section 200 Grid 200f Mounting surface W Water (liquid)
WL liquid level

Claims (5)

容器本体と、
前記容器本体における第１水平方向の一端部に設けられ、試料から切片を切り出すナイフ部と、
前記ナイフ部で切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、
前記切片を載置する載置面を有した板状のグリッドが、前記載置面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、
前記第１水平方向と直交する第２水平方向を前記容器本体の幅方向としたとき、前記グリッド収容部が、前記容器本体の幅方向両側にそれぞれ設けられている、切片作製部材。 With the container body
A knife portion provided at one end in the first horizontal direction of the container body for cutting a section from a sample, and a knife portion.
A liquid storage part in which the liquid in which the section cut out by the knife part floats is stored, and a liquid storage part.
A plate-shaped grid having a mounting surface on which the section is placed is a grid accommodating portion in which the above-mentioned mounting surface is inclined and accommodated in a state of being obliquely upward, and the liquid storage portion and the liquid are accommodated. With a grid accommodating section that communicates in the area containing the liquid level of
A section preparation member in which the grid accommodating portions are provided on both sides of the container body in the width direction when the second horizontal direction orthogonal to the first horizontal direction is defined as the width direction of the container body . 前記グリッド収容部が、前記グリッドの一部を前記液体貯留部に貯留される前記液体の液面よりも上方に突出させた状態で前記グリッドを収容する請求項１に記載の切片作製部材。 The section preparation member according to claim 1, wherein the grid accommodating portion accommodates the grid in a state in which a part of the grid is projected upward from the liquid level of the liquid stored in the liquid storage portion. 容器本体と、
試料から切片を切り出すナイフ部と、
前記ナイフ部で切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、
前記切片を載置する板状のグリッドが、その厚さ方向の一方の面である第１の面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、
前記グリッド収容部が、前記グリッドの一部を前記液体貯留部に貯留される前記液体の前記液面よりも上方に突出させた状態で前記グリッドを収容するように構成され、
前記グリッド収容部が、前記グリッドを、前記第１の面の下側が第１の壁部に突き当てられ、前記グリッドの前記厚さ方向の他方の面である第２の面の上側が第２の壁部によって支持されることによって、前記第１の面が前記ナイフ部側に向かって漸次下方に傾斜した状態で収容するように構成されている、切片作製部材。 With the container body
The knife part that cuts out the section from the sample,
A liquid storage part in which the liquid in which the section cut out by the knife part floats is stored, and a liquid storage part.
The plate-shaped grid on which the section is placed is a grid accommodating portion in which the first surface, which is one surface in the thickness direction thereof, is inclined and accommodated in a state of being obliquely upward, and the liquid storage portion. A grid accommodating portion that communicates with the portion and the region including the liquid level of the liquid is provided.
The grid accommodating portion is configured to accommodate the grid in a state in which a part of the grid is projected upward from the liquid level of the liquid stored in the liquid storage portion.
The grid accommodating portion abuts the grid against the first wall portion on the lower side of the first surface, and the upper side of the second surface, which is the other surface in the thickness direction of the grid, is the second. A sectioning member configured to accommodate the first surface in a state of being gradually inclined downward toward the knife portion side by being supported by the wall portion of the . 前記グリッド収容部が、水平面を基準とした前記グリッドの傾斜角度が４０°以上となるように収容する、請求項３に記載の切片作製部材。The section preparation member according to claim 3, wherein the grid accommodating portion accommodates the grid so that the inclination angle of the grid with respect to the horizontal plane is 40 ° or more. 水平方向において一端部および他端部を有する容器本体と、
前記容器本体における前記一端部に設けられ、試料から切片を切り出すナイフ部と、
前記容器本体内に設けられ、前記ナイフ部によって切り出された前記切片が浮かぶ液体が貯留される液体貯留部と、
前記切片を載置する載置面を有した板状のグリッドが、前記載置面を斜め上方に向けた状態で傾斜して収容されるグリッド収容部であって、前記液体貯留部と前記液体の液面を含む領域で連通するグリッド収容部と、を備え、
前記グリッド収容部が、前記液体貯留部の前記水平方向の中央よりも前記一端部に近い側に設けられている、切片作製部材。 A container body having one end and the other end in the horizontal direction,
A knife portion provided at one end of the container body for cutting a section from a sample, and a knife portion.
A liquid storage unit provided in the container body and storing a liquid in which the section cut out by the knife unit floats, and a liquid storage unit.
A plate-shaped grid having a mounting surface on which the section is placed is a grid accommodating portion in which the above-mentioned mounting surface is inclined and accommodated in a state of being obliquely upward, and the liquid storage portion and the liquid are accommodated. With a grid accommodating section that communicates in the area containing the liquid level of
A section preparation member in which the grid accommodating portion is provided on a side closer to the one end portion than the horizontal center of the liquid storage portion.
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KR102678906B1 (en) * 2023-01-04 2024-06-28 주식회사 다인테크 Diamond knife for microtome with angle adjustable knife blade

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7015077B1 (en) * 2020-11-06 2022-02-02 株式会社シンテック Section making device and section making device set

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3016900U (en) 1995-03-28 1995-10-09 有限会社マイクロスター Cryo-dry diamond knife for electron microscope frozen section preparation
JP2007033312A (en) 2005-07-28 2007-02-08 Canon Inc Method and device for manufacturing segment sample for analysis
US20150338316A1 (en) 2014-05-21 2015-11-26 Zhongwei Chen Microtomic System and Process Utilizing Electrostatic Force to Handle Sample Sections

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0797069B2 (en) * 1993-05-14 1995-10-18 財団法人東京都神経科学総合研究所 Method and device for collecting and holding continuous thin section for microscope
JPH0921733A (en) * 1995-07-05 1997-01-21 Kao Corp Method and auxiliary tool for collecting sample

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3016900U (en) 1995-03-28 1995-10-09 有限会社マイクロスター Cryo-dry diamond knife for electron microscope frozen section preparation
JP2007033312A (en) 2005-07-28 2007-02-08 Canon Inc Method and device for manufacturing segment sample for analysis
US20150338316A1 (en) 2014-05-21 2015-11-26 Zhongwei Chen Microtomic System and Process Utilizing Electrostatic Force to Handle Sample Sections

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102678906B1 (en) * 2023-01-04 2024-06-28 주식회사 다인테크 Diamond knife for microtome with angle adjustable knife blade

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