JP7094652B2 - Oil and fat composition for bread making and flour dough for bread making - Google Patents

Description

The present invention produces breads having a good texture and an excellent anti-aging effect by blending hemicellulase that acts near the gelatinization temperature and maltose-forming α-amylase that acts above the gelatinization temperature of starch. With respect to the improver for bread flour dough that can be made.

Breads containing starch as a main component age over time after baking, resulting in drying and deterioration of softness. For example, in Patent Documents 1 to 4, the softness of bread is improved by using emulsifiers such as glycerin fatty acid ester and propylene glycol fatty acid ester, but it is insufficient to maintain the moistness and softness after 3 or 4 days of baking. Therefore, it is also known that the use of an emulsifier causes fluffing and spoiling the flavor on the first day of baking. In addition, this method cannot meet the customer's preference for additive-free products in recent years.

On the other hand, there is also known a method of suppressing bread aging by combining various enzymes including lipase, cellulase, amylase, glucose oxidase and the like. For example, in Patent Document 5, it is possible to soften bread by using lipase, hemicellulase, and amylase in combination without using an emulsifier. However, lipase has problems such as decomposing fats and oils and producing an offensive odor. Further, in Patent Document 6, lipase, glucose oxidase, β-galactosidase, amylase, cellulase and the like are used in combination, but there is a problem similar to the above.

In Patent Document 7, hemicellulase and amylase derived from Aspergillus oryzae are used as an improving agent for frozen bread dough, but amylase derived from Aspergillus oryzae has an optimum temperature of 50 to 60 ° C. in a relatively low temperature range. Therefore, when it is used as an improving agent for ordinary bread dough instead of frozen dough, there arises a problem in terms of workability such as stickiness of bread dough. In addition, since amylase cuts the skeleton of starch, it can dramatically improve the softness after baking, but it makes the bread one day after baking excessively soft, and as a result, it is uncomfortable. It becomes bread. If an improving agent is added so as not to cause these problems, it is insufficient to maintain the softness after 3 days of baking.

Patent Document 8 is a technique that enables long-term storage of bread by using α-amylase and maltose-producing α-amylase in combination, but the effect is insufficient only with maltose-producing α-amylase, and α-amylase and α-amylase It is said that the combined use of is essential. However, the combined use of α-amylase causes the same problems as described above.

Thus, in order to obtain sufficiently soft bread even on the third day without using an emulsifier, it was indispensable to use α-amylase or to use α-amylase in combination with other enzymes. In this case, baking is performed. After one day, the bread became too soft, and there were problems such as poor chewy texture and a messy texture that did not melt in the mouth. In addition, the aging of starch is particularly severe in breads containing ingredients with rapid moisture transfer and breads with biscuit dough, and existing techniques are not sufficient to maintain the softness of bread even after 3 or 4 days of baking. there were.

As described above, while maintaining good workability of the flour dough for bread making before baking, bread can be baked without impairing the goodness of firmness and melting in the mouth without using an emulsifier, and the aging of bread is effective. There is a demand for an oil / fat composition for bread making that can maintain softness and moistness for a long period of time with a delay.

Japanese Unexamined Patent Publication No. 03-11948 Japanese Unexamined Patent Publication No. 03-292846 Japanese Unexamined Patent Publication No. 04-207143 Japanese Unexamined Patent Publication No. 2015-181434 Japanese Unexamined Patent Publication No. 06-169681 Japanese Unexamined Patent Publication No. 2002-272357 Japanese Unexamined Patent Publication No. 2000-083573 Japanese Unexamined Patent Publication No. 2010-148487

In the present invention, bread can be baked without impairing the goodness of firmness and melting in the mouth while maintaining good workability of the flour dough for bread making before baking, effectively delaying the aging of bread and making it soft and moist. The challenge is to develop an improving agent for flour dough for bread making that can maintain this for a long period of time.

As a result of diligent studies on the combined effect of various enzymes, the above-mentioned problems are solved by using hemicellulase and maltose-forming α-amylase, which have an optimum temperature in a specific temperature range with respect to the gelatinization temperature of starch. This finding has led to the completion of the present invention.
That is, the present invention is the following [1] to [4].

[1] Contains 100 g of edible oil and fat, hemicellulase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-producing α-amylase (mA) having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. A bread-making oil / fat composition containing 1 to 100 u of hemicellulase (H) and 50 to 5000 u of maltose-forming α-amylase (mA).
[2] The maltose-producing α-amylase (mA) is a maltose-producing α-amylase (mA1) having an optimum temperature of 65 ° C. or higher and lower than 75 ° C. and a maltose-forming α-amylase (mA1) having an optimum temperature of 75 ° C. or higher and 85 ° C. or lower. The oil and fat composition for bread making according to the above [1], which comprises mA2).

[3] Contains flour, edible oil and fat, hemicellulase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-forming α-amylase (mA) having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. , 0.000005 to 0.0005 parts by mass of hemicellulase (H) and 0.0005 to 0.05 parts by mass of maltose-producing α-amylase (mA) with respect to 100 parts by mass of flour. material.
[4] The maltose-producing α-amylase (mA) is a maltose-producing α-amylase (mA1) having an optimum temperature of 65 ° C. or higher and lower than 75 ° C. and a maltose-forming α-amylase (mA1) having an optimum temperature of 75 ° C. or higher and 85 ° C. or lower. The flour dough for bread making according to the above [3], which comprises mA2).

According to the present invention, it is possible to obtain a flour dough for bread making which has good workability before baking and can maintain softness for a long period of time without deteriorating the flavor, chewyness and texture after baking.

Hereinafter, the present invention will be described in more detail.
The oil and fat composition for bread making of the present invention comprises edible oil and fat, hemicellulase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-forming α-amylase having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. Contains (mA).

(Hemicellulase (H))
The hemicellulase (H) used in the present invention is characterized by having an optimum temperature of 45 to 60 ° C, preferably 50 to 60 ° C. Hemicellulase is an enzyme that hydrolyzes polysaccharides contained in plant tissues, and includes xylanase that hydrolyzes xylan, arabanase that hydrolyzes alaban, and mannase that hydrolyzes mannan. Any of these may be selected, but xylanase is particularly preferably selected and added. Hemicellulase (H) is of fungal origin (eg, Trichoderma, Meripils, Humicola, Aspergillus, Fusarium) or bacterial origin (eg, Bacillus). One type or two or more types of these hemicellulases may be selected and used.

In the present invention, hemicellulase (H) has an optimum temperature near the gelatinization temperature of starch, so that the polysaccharide is produced by the hemicellulase (H) decomposing the polysaccharide at the same time as the gelatinization of starch progresses. The contained water is released into the bread dough and can be efficiently used for gelatinization of starch. When the gelatinization of starch progresses sufficiently, the maltose-producing α-amylase (mA) can act on the starch, so that the effect of the maltose-producing α-amylase (mA) can be efficiently enhanced.

In the fat and oil composition for bread making of the present invention, the content of hemicellulase (H) is 1 to 100u, preferably 10 to 50u in 100 g of the fat and oil composition for bread making. If it is less than 1u, a sufficient anti-aging effect cannot be obtained, and if it exceeds 100u, it causes deterioration of workability and deterioration of the quality of the obtained bread.

(Maltose-producing α-amylase (mA))
The maltose-forming α-amylase (mA) used in the present invention is characterized by having an optimum temperature of 65 to 85 ° C. and a deactivation temperature of 80 to 100 ° C. Maltose-producing α-amylase is an enzyme that mainly produces maltose by hydrolyzing α-1,4-glucosidic bonds, and is derived from bacteria such as Bacillus, grains such as Malt, and molds such as Aspergillus. Any of these can be used. Further, preferably, a maltose-producing α-amylase (mA1) having an optimum temperature of 65 ° C. or higher and lower than 75 ° C. and a maltose-forming α-amylase (mA2) having an optimum temperature of 75 ° C. or higher and 85 ° C. or lower can be used in combination. .. By using two types with different optimum temperatures in combination, a decomposition action can be obtained continuously on the starch in the bread dough in a wide temperature range from low temperature to high temperature, and it works sufficiently on the starch. , The anti-aging effect of baked bread is further improved.

In the bread-making oil / fat composition of the present invention, the content of maltose-producing α-amylase (mA) is the sum of maltose-forming α-amylase (mA1) and maltose-producing α-amylase (mA2), and the content of maltose-producing α-amylase (mA2) is the sum of the maltose-forming α-amylase (mA2). It is 50 to 5000 u, preferably 500 to 2000 u in 100 g of the product. If it is less than 50u, a sufficient antiaging effect cannot be obtained, and if it exceeds 5000u, it causes deterioration of workability and deterioration of the quality of the obtained bread.
Further, in the fat and oil composition for bread making of the present invention, the content of maltose-producing α-amylase (mA1) is preferably 30 to 2000 u, preferably 250 to 1000 u, based on 100 g of the fat and oil composition. More preferred. The content of maltose-producing α-amylase (mA2) is preferably 30 to 4000 u and more preferably 500 to 2000 u with respect to 100 g of the fat and oil composition.

(Activity unit)
The active units of hemicellulase (H) and maltose-producing α-amylase used in the present invention are defined as 1 u in the amount of an enzyme that produces 1 μmol of xylose and a reducing sugar corresponding to maltose, respectively, in 1 minute. The enzyme activity of hemicellulose (H) can be determined by reacting hemicellulose with hemicellulose as a substrate under optimum conditions (optimal temperature, optimum pH) for 10 minutes and quantifying the produced reducing sugar. For maltose-producing α-amylase, the enzyme activity can be determined by reacting maltotriose as a substrate under the optimum conditions (optimum temperature, optimum pH) for 10 minutes and quantifying the produced reducing sugar. I can. Each reducing sugar can be quantified by referring to "Reducing sugar quantification method (2nd edition)" (written by Sakuzo Fukui, Academic Society Publishing Center).

(Optimal temperature of enzyme)
In the present invention, the optimum temperature of the enzyme means the temperature having the highest activity as a result of dissolving the enzyme in water, changing the temperature by 5 ° C. and measuring the activity. Further, in the present invention, the deactivation temperature means a temperature at which the relative activity is 10% or less with respect to the optimum temperature as a result of dissolving the enzyme in water, changing the temperature and measuring the activity.

(Edible oils and fats)
As the edible oil / fat used in the present invention, edible oil / fat generally used as a raw material for margarine and shortening can be used. For example, animal fats and oils such as beef tallow, pork fat and fish oil, vegetable fats and oils such as palm oil, rapeseed oil and soybean oil, and processed fats and oils such as these animal fats and oils, hydrogenated vegetable fats and oils, fractionated oils and ester exchange oils. These can be mixed and used as appropriate. In the present invention, the edible fat and oil preferably has a solid fat content (SFC) of 10 to 30% at the dough temperature in the mixing step of bread making.

Emulsifiers, modified starches, preservatives, pH adjusters, pigments, flavors, other enzymes and the like may be appropriately used in the oil and fat composition for bread making in the present invention.
As the emulsifier, for example, glycerin fatty acid ester, glyceride fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, lecithin, polyglycerin condensed fatty acid ester and the like are used. The emulsifier can be used alone or in combination of two or more, but glycerin fatty acid ester is preferable, and fatty acid monoglyceride alone with saturated or unsaturated fatty acids having an effect of improving texture and an effect of preventing aging of starch is particularly preferable. Alternatively, it is preferable to use a mixture of saturated or unsaturated fatty acid monoglyceride and another emulsifier because the texture improving effect and the antiaging effect are further improved.
The amount of the emulsifier added is usually 0.1 to 10 parts by mass, preferably 0.3 to 3 parts by mass with respect to 100 parts by mass of edible oil and fat.

In the method for producing an oil / fat composition in the present invention, the oil / fat and the oil-soluble component are first heated at a temperature equal to or higher than the melting point temperature, uniformly melted, and then lowered to 50 to 55 ° C. Next, warm water is added, the mixture is uniformly mixed and stirred, an enzyme is added, the mixture is rapidly cooled and plasticized using a prototype, and cooled to 30 ° C. or lower to obtain the desired oil / fat composition. In the above production, when cooling the homogeneous mixture in a high temperature state, the container itself containing the homogeneous mixture may be cooled from the outside, but a chiller, a botator, and a combinator generally used for shortening and margarine production may be used. It is preferable in terms of performance to quench the mixture by using or the like.
In producing the oil and fat composition of the present invention, the enzyme may be in any form such as powder or liquid.

The flour dough for bread making of the present invention comprises flour, edible oil and fat, hemicellase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-forming α- having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. Contains amylase (mA). The content of each is 0.000005 to 0.0005 parts by mass of hemicellulase (H) and 0.0005 to 0.05 parts by mass of maltose-producing α-amylase (mA) with respect to 100 parts by mass of flour. be. If the amount added is too small, a sufficient anti-aging effect cannot be obtained, and if the amount added is too large, the texture of the obtained bakery product deteriorates.

Further, when the content of each enzyme in the flour dough for bread making of the present invention is expressed in units, the content of hemicellulase (H) is preferably 0.05 to 5u with respect to 100 g of flour, more preferably. It is 0.5 to 2u.
The content of maltose-producing α-amylase (mA) is preferably 5 to 500u, more preferably 5 to 500u, as a total of maltose-producing α-amylase (mA1) and maltose-producing α-amylase (mA2) with respect to 100 g of flour. It is 50 to 200u.
Furthermore, the content of maltose-producing α-amylase (mA1) is preferably 1 to 300 u, more preferably 25 to 100 u, with respect to 100 g of flour. The content of maltose-producing α-amylase (mA2) is preferably 1 to 400, more preferably 50 to 200u, with respect to 100 g of flour.

Hemicellulase (H) and maltose-producing α-amylase (mA) can be added by being contained in the oil and fat composition for bread making of the present invention. In the present invention, the amount of the oil / fat composition of the present invention added at the time of preparing the bakery product is usually 1 to 35 parts by mass, preferably 5 to 15 parts by mass with respect to 100 parts by mass of the flour used in the bakery product. By setting the amount of the oil / fat composition in this range, a sufficient anti-aging effect and a good texture of the bakery product can be obtained.

As raw materials for bakery products in the present invention, in addition to grain flour as the main raw material, yeast, yeast food, emulsifiers, fats and oils (shortening, lard, margarine, butter, liquid oil, etc.), water, processed starch, dairy products, etc. Examples thereof include salt, sugars, seasonings (sodium glutamate and nucleic acids), preservatives, fortifiers such as vitamins and calcium, proteins, amino acids, chemical swelling agents, flavors and the like. Furthermore, dried fruits such as raisins, wheat bran, whole grain flour and the like, which generally tend to age when used as raw materials, can be used.

Breads produced using the oil and fat composition of the present invention include breads filled with fillings and the like, and examples thereof include breads, special breads, cooked breads, and sweet breads. Specific examples of bread include white bread, black bread, French bread, variety bread, and rolls (table rolls, buns, butter rolls, etc.). Special breads include muffins, cooking breads include hot docks and hamburgers, and sweet buns include jam breads, anpan breads, cream buns, raisin breads, and melon breads.

Next, the present invention will be specifically described with reference to examples.
[Preparation of oil and fat composition for bread making]
The enzyme raw materials shown in Tables 2 and 3 were added to the bread-making oil / fat composition base having the composition shown in Table 1 to obtain a bread-making oil / fat composition. The method for producing an oil / fat composition for bread making is as follows.
20 kg of warmed water is added to the oil phase portion in which 5 kg of hydrogenated palm oil (melting point 42 ° C.), 30 kg of palm oil, 35 kg of hardened rapeseed oil (melting point of 36 ° C.), 30 kg of rapeseed oil and 100 g of soybean lecithin are mixed and melted. An emulsion was produced. The temperature of the emulsion was lowered to 50 to 55 ° C., the enzyme raw material was added, the mixture was sufficiently stirred, and then the mixture was rapidly cooled to 30 ° C. or lower using a margarine prototype to prototype an oil / fat composition for bread making.
Tables 2 and 3 show the content of each enzyme in each of the obtained fat and oil compositions for bread making. The numerical value in the upper part of the table is the mass (g) of the raw material enzyme contained in 100 g of the oil / fat composition for bread making, and the numerical value in parentheses in the lower part is the value of the enzyme contained in 100 g of the oil / fat composition for bread making. The amount of activity (/ u). Each of the obtained oil and fat compositions for bread making is marked with a symbol and shown at the lower ends of Tables 2 and 3.

(Enzyme raw material)
<Hemicellulase>
1) Product name: Grindamyl H460, manufactured by Danisco Japan Co., Ltd., optimum temperature 45 ° C.
2) Product name: Bakeryme BXPJ, manufactured by DSM Co., Ltd., optimum temperature 50 ° C.
3) Product name: Sumiteam X, manufactured by Shin Nihon Kagaku Co., Ltd., optimum temperature 55 ° C
4) Product name: Sucrase X, manufactured by Mitsubishi Chemical Foods Co., Ltd., optimum temperature 60 ° C
<Α-amylase>
5) Product name: Fungamyl, manufactured by Novozyme Japan Co., Ltd., optimum temperature 55 ° C
<Maltose-producing α-amylase>
6) Product name: Novamyl, manufactured by Novozyme Japan Co., Ltd., optimum temperature 70 ° C
7) Product name: Novamyl 3D, manufactured by Novozyme Japan Co., Ltd., optimum temperature 75 ° C

[Bakery evaluation]
Bread was produced using the above oil / fat composition for bread making, and the bread making property was evaluated. In the evaluation of bread-making properties, five evaluation items were set: workability of the dough, aging resistance, firmness, melting in the mouth, and moistness. The evaluation method for each evaluation item is described below.
The bread making test was performed on bread, raisin bread, melon bread, and raisin roll.

(Evaluation method of fabric workability)
The use of enzymes and emulsifiers can cause problems such as the dough becoming loose and sticky, or vice versa. The normal good dough condition is "3", the dough is quite sticky as "5", the dough is slightly sticky as "4", the dough is slightly loose as "2", and the dough is quite sticky. The case was evaluated as "1". The grades "2", "3", and "4" were passed.

(Evaluation method of aging resistance)
On the 1st day (D + 1) and the 4th day (D + 4) after firing, the change over time in the softness of the punkram was measured, and those with a smaller change in softness than the control were evaluated as having strong aging resistance. .. For bread, raisin bread, and melon bread, the crumbs of the bread on the first and fourth days after baking were sliced 3 cm from the bottom surface, and the central part was cut into a square of 4 cm × 4 cm to prepare a sample. The stress (N) required to compress the crumb by 1.5 cm from the sliced surface was measured with a Sanyo Electric Railway Leometer and used as an index of softness. For raisin rolls, the center of the bread on the 1st and 4th days after baking was sliced by 3 cm to prepare a sample. The stress (N) required to compress the crumb by 1.5 cm from the upper surface of the pan crumb was measured with a leometer manufactured by Sanyo Electric Railway Co., Ltd. and used as an index of softness. Compared with the case where the oil / fat composition C is used as a control that does not contain an enzyme, the case where the amount of change in softness is 1 time or more is "1", and the case where the change amount is 0.9 times or more and less than 1 time. "2", 0.8 times or more and less than 0.9 times is "3", 0.7 times or more and less than 0.8 times is "4", less than 0.7 times The case was set to "5" and the evaluation was performed on a 5-point scale. A score of "4" or higher was considered as a pass.

(Evaluation method of waist holding)
The specific volume of the bread was measured on the first day after baking, and the bread with no decrease in volume as compared with the control was evaluated as having good waist retention. The specific volume of the bread was measured using a 3D laser volume meter manufactured by Astex. "5" when the specific volume is larger than 1.05 times and "4" when it is larger than 1 times and 1.05 times or less, and "3" when it is larger than 0.95 times and 1 times or less compared to the control. , The case of greater than 0.9 times and 0.95 times or less was regarded as "2", and the case of 0.9 times or less was regarded as "1", and the evaluation was performed on a 5-point scale. A score of "3" or higher was considered as a pass.

(Evaluation method of melting in the mouth and moistness)
The feeling of melting of bread on the first day after baking was evaluated by 15 panelists. Evaluation items were set for very good melting in the mouth (5), good (4), normal (3), slightly lumpy (2), and lumpy (1), and the item with the largest number of people was the feeling of melting in the mouth. did. In addition, the moistness of the bread on the third day after baking was evaluated by 15 panelists. The number of people in the punk lamb was the largest, with evaluation items of very moist (5), slightly moist (4), normal (3), slightly dry (2), and dry (1). I made the item moist. In both cases, a score of 3 or higher was passed.

<Bread evaluation>
Bread was baked with the following composition (Table 4) and evaluated according to the above evaluation method. Table 5 shows the symbols of the oil and fat composition for bread making blended in the flour dough for bread making, and summarizes the amount of enzyme and the evaluation results in the flour dough for bread making.

(Bread evaluation result)

From Table 5, it can be seen that when the oil / fat composition P1 or the oil / fat composition P2 is used, a sufficient anti-aging effect and moistness can be obtained without impairing the bread-making property. On the other hand, the fat and oil composition R1 containing no maltose-forming α-amylase did not affect the bread making property, but the result was almost the same as that of the control in terms of aging resistance and moistness.

<Evaluation of raisin bread>
The raisin bread was baked with the following composition (Table 6) and evaluated according to the above evaluation method. Table 7 shows the symbols of the oil and fat composition for bread making blended in the flour dough for bread making, and summarizes the amount of enzyme and the evaluation results in the flour dough for bread making.

(Raisin bread evaluation result)

From Table 7, when the oil / fat composition P3 was used, some stickiness was observed in the bread dough, but strong aging resistance and moistness were imparted. Further, in the oil / fat composition P4, a very strong aging resistance and a moist texture were obtained without impairing the bread making property. On the other hand, in the oil / fat composition R2 using α-amylase having a low optimum temperature, the dough was sticky and the bread after baking had a crunchy texture. Further, when the oil / fat composition R3 containing no hemicellulase (xylanase) was used, a sufficient anti-aging effect could not be obtained.

<Evaluation of melon bread>
Melon bread was baked with the following composition (Table 8) and evaluated according to the above evaluation method. Table 9 shows the symbols of the oil and fat composition for bread making blended in the flour dough for bread making, and summarizes the amount of enzyme and the evaluation results in the flour dough for bread making.

(Melon bread evaluation result)

From Table 9, when the oil and fat compositions P5 and P6 were used, some stickiness was observed in the dough, but a sufficient anti-aging effect and moistness were imparted. On the other hand, in the fat and oil composition R4 containing a large amount of hemicellulase and the fat and oil composition R5 containing a large amount of maltose-forming α-amylase, excessive stickiness of the dough was observed. In addition, the bread had a messy texture due to its poor waist.

<Raisin roll evaluation>
Raisin rolls were fired with the following formulation (Table 10) and evaluated according to the above evaluation method. Table 11 shows the symbols of the oil and fat composition for bread making blended in the flour dough for bread making, and summarizes the amount of enzyme and the evaluation results in the flour dough for bread making.

(Raisin roll evaluation result)

From Table 11, some of the oil and fat compositions P3, P4, and P7 showed some stickiness, but sufficient aging resistance and strong moistness were obtained. On the other hand, in the fat and oil composition R2, as in the case of raisin bread and the like, deterioration in bread making property and deterioration in bread quality were observed.
Based on the above, the bread-making oil / fat composition of the present invention provides good workability before baking regardless of the type of bread, and provides long-term softness without deteriorating the flavor, firmness, and texture after baking. It is clear that a sustainable bread flour dough can be obtained, and a bread-making oil / fat composition other than the present invention cannot obtain the desired effect.

Claims (4)

It contains edible fats and oils, hemicellulase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-forming α-amylase (mA) having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. It contains 1 to 100 u of hemicellulase (H) and 50 to 5000 u of maltose-forming α-amylase (mA) .
An oil / fat composition for bread making used for setting the content of hemicellulase (H) to 0.05 to 8u and the content of maltose-forming α-amylase (mA) to 5 to 500u with respect to 100 g of flour . Maltose-producing α-amylase (mA) includes maltose-producing α-amylase (mA1) having an optimum temperature of 65 ° C. or higher and lower than 75 ° C. and maltose-forming α-amylase (mA2) having an optimum temperature of 75 ° C. or higher and 85 ° C. or lower. The oil and fat composition for bread making according to claim 1. Flour 100 contains flour, edible oil and fat, hemicellulase (H) having an optimum temperature of 45 ° C. or higher and 60 ° C. or lower, and maltose-producing α-amylase (mA) having an optimum temperature of 65 ° C. or higher and 85 ° C. or lower. A flour dough for bread making having a hemicellulase (H) of 0.05 to 8u and a maltose-forming α-amylase (mA) of 5 to 500u with respect to g . Maltose-producing α-amylase (mA) includes maltose-producing α-amylase (mA1) having an optimum temperature of 65 ° C. or higher and lower than 75 ° C. and maltose-forming α-amylase (mA2) having an optimum temperature of 75 ° C. or higher and 85 ° C. or lower. The flour dough for bread making according to claim 3.