JP7075500B2 - 異なるサイズの細胞を移送するための微小流体チャネル - Google Patents
異なるサイズの細胞を移送するための微小流体チャネル Download PDFInfo
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Description
流体内の細胞を分注(分配)する精度と信頼性は、供給源からの流体を分注ノズルに移送する微小流体流路の構造に依存する場合がある。微小流体流路は、特定のタイプの細胞に良好に機能しない場合がある。従って、細胞は、確実に分注されない場合があるか、又は流路内に蓄積して障害物を形成する場合がある。例えば、普通より小さい微小流体流路を用いて大型の細胞を分注することは、障害につながる場合があり、分注が阻止される場合がある。微小流体流路が大き過ぎる場合、細胞は必要以上に自由に分注される場合があり、特に単一細胞アッセイにおいて無駄にされる場合がある。操作者は、特定の細胞の連続する流体に対して微小流体チャネルの性能を予測することを試みる場合がある。しかしながら、これは、操作者に高いレベルの技能または経験を要求し、操作者が、多数の異なるように構成された微小流体デバイスの中から選ぶことを必要とする場合がある。
Claims (13)
- 流体リザーバから細胞含有流体を受け取るために前記流体リザーバと連絡し、第1のサイズ範囲の細胞を移送するように形作られた第1の微小流体チャネルと、
前記流体リザーバから細胞含有流体を受け取るために前記流体リザーバと連絡し、前記第1のサイズ範囲と異なる第2のサイズ範囲の細胞を移送するように形作られた第2の微小流体チャネルと、
前記第1の微小流体チャネルに配置された第1のセンサと、
前記第2の微小流体チャネルに配置された第2のセンサと、
前記第1の微小流体チャネルの端部に配置された第1の分注ノズルと、
前記第2の微小流体チャネルの端部に配置された第2の分注ノズルと、
前記第1の微小流体チャネルにおける前記第1のセンサに接続するように構成され、前記第2の微小流体チャネルにおける第2のセンサに接続するように更に構成されたコントローラと、を含み、
前記コントローラは、前記第1の微小流体チャネルから細胞を分注するために前記第1の微小流体チャネルにおける前記第1の分注ノズルを駆動するように更に構成され、前記第2の微小流体チャネルから細胞を分注するために前記第2の微小流体チャネルにおける前記第2の分注ノズルを駆動するように更に構成され、
前記コントローラは、前記第1のセンサから第1の信号を取得し且つ前記第2のセンサから第2の信号を取得するように更に構成され、前記コントローラは、前記第1の信号および前記第2の信号のどちらが細胞の所望の分注特性により近いかを判定し、前記第1の信号および前記第2の信号の判定に基づいて、アクティブな細胞分注ノズルとして前記第1の分注ノズル又は前記第2の分注ノズルを選択するように更に構成されている、デバイス。 - 前記コントローラは、前記第1の微小流体チャネル又は前記第2の微小流体チャネル内の細胞含有流体内に含まれる細胞を分注するために、前記アクティブな細胞分注ノズルを更に駆動することができる、請求項1に記載のデバイス。
- 前記コントローラは更に、選択されない分注ノズルの駆動信号の出力を停止することができる、請求項1又は2に記載のデバイス。
- 前記コントローラは、異なるターゲット場所に対して前記アクティブな細胞分注ノズルの位置を制御することができ、前記コントローラは、異なるターゲット場所のそれぞれにおいて細胞を分注するために前記アクティブな細胞分注ノズルに対応するセンサ信号を参照することができる、請求項1~3の何れか1項に記載のデバイス。
- 前記コントローラは、前記アクティブな細胞分注ノズルを選択するために、前記第1の信号を前記第2の信号と比較することができる、請求項1~4の何れか1項に記載のデバイス。
- 前記コントローラは、前記アクティブな細胞分注ノズルを選択するために、前記第1の信号および前記第2の信号を基準信号と比較することができる、請求項1~4の何れか1項に記載のデバイス。
- 前記第1の微小流体チャネル、前記第1のセンサ、前記第1の分注ノズル、前記第2の微小流体チャネル、前記第2のセンサ、及び前記第2の分注ノズルを含む着脱可能なカセットを受容するためのカセットホルダを更に含む、請求項1~6の何れか1項に記載のデバイス。
- 前記第1のセンサの検出近傍へ細胞を案内するために、前記第1の微小流体チャネルへ延びる第1の表面特徴要素を更に含む、請求項1~7の何れか1項に記載のデバイス。
- 前記第2のセンサの検出近傍へ細胞を案内するために、前記第2の微小流体チャネルへ延びる第2の表面特徴要素を更に含む、請求項1~8の何れか1項に記載のデバイス。
- 分注ヘッドと、
前記分注ヘッドに電気接続するためのコントローラと、を含み、
前記分注ヘッドは、
異なる細胞サイズの選択性を有する複数の微小流体チャネルと、
複数のセンサであって、前記複数のセンサのそれぞれが前記複数の微小流体チャネルのそれぞれに配置されている、複数のセンサと、
複数の分注ノズルであって、前記複数の分注ノズルのそれぞれが前記複数の微小流体チャネルのそれぞれに配置されている、複数の分注ノズルと、を含み、
前記コントローラは、前記複数の微小流体チャネルのそれぞれに配置された各センサから求められた細胞含有流体の流れの検出された特性に基づいて、前記複数の分注ノズルからアクティブな分注ノズルを選択するように構成され、前記アクティブな分注ノズルから細胞含有流体を分注するように構成される、デバイス。 - 前記コントローラは、選択されない分注ノズルからの細胞含有流体の分注を更に停止することができる、請求項10に記載のデバイス。
- 前記分注ヘッドを含む着脱可能なカセットを受容するためのカセットホルダを更に含む、請求項10~11の何れか1項に記載のデバイス。
- 前記コントローラは、前記分注ヘッドを含む複数の分注ヘッドに電気接続することができる、請求項10~12の何れか1項に記載のデバイス。
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PCT/US2018/025839 WO2019194790A1 (en) | 2018-04-03 | 2018-04-03 | Microfluidic channels to convey cells of different sizes |
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- 2018-04-03 US US16/607,769 patent/US11278888B2/en active Active
- 2018-04-03 KR KR1020207031308A patent/KR20200138352A/ko not_active Application Discontinuation
- 2018-04-03 WO PCT/US2018/025839 patent/WO2019194790A1/en unknown
- 2018-04-03 EP EP18913548.6A patent/EP3759486A1/en active Pending
- 2018-04-03 CN CN201880093011.5A patent/CN112041676B/zh active Active
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JP2021517468A (ja) | 2021-07-26 |
EP3759486A4 (en) | 2021-01-06 |
KR20200138352A (ko) | 2020-12-09 |
CN112041676A (zh) | 2020-12-04 |
WO2019194790A1 (en) | 2019-10-10 |
EP3759486A1 (en) | 2021-01-06 |
US20210069705A1 (en) | 2021-03-11 |
US11278888B2 (en) | 2022-03-22 |
CN112041676B (zh) | 2022-12-13 |
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