JP7049103B2 - 単一細胞の網羅的３’末端遺伝子発現解析法 - Google Patents

単一細胞の網羅的３’末端遺伝子発現解析法 Download PDF

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JP7049103B2
JP7049103B2 JP2017236839A JP2017236839A JP7049103B2 JP 7049103 B2 JP7049103 B2 JP 7049103B2 JP 2017236839 A JP2017236839 A JP 2017236839A JP 2017236839 A JP2017236839 A JP 2017236839A JP 7049103 B2 JP7049103 B2 JP 7049103B2
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sequence
cdna
cell
solid phase
carrier
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JP2019103415A (ja
JP2019103415A5 (https=
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妃代美 谷口
正敬 白井
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Hitachi Ltd
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Hitachi Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2017236839A 2017-12-11 2017-12-11 単一細胞の網羅的３’末端遺伝子発現解析法 Expired - Fee Related JP7049103B2 (ja)

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Application Number Priority Date Filing Date Title
JP2017236839A JP7049103B2 (ja) 2017-12-11 2017-12-11 単一細胞の網羅的３’末端遺伝子発現解析法
US16/769,434 US20200385712A1 (en) 2017-12-11 2018-11-09 Method for comprehensively analyzing 3' end gene expression of single cell
PCT/JP2018/041702 WO2019116800A1 (ja) 2017-12-11 2018-11-09 単一細胞の網羅的３'末端遺伝子発現解析法

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JP2017236839A JP7049103B2 (ja) 2017-12-11 2017-12-11 単一細胞の網羅的３’末端遺伝子発現解析法

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JP2019103415A JP2019103415A (ja) 2019-06-27
JP2019103415A5 JP2019103415A5 (https=) 2020-11-12
JP7049103B2 true JP7049103B2 (ja) 2022-04-06

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Publication number Priority date Publication date Assignee Title
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
JP2023183556A (ja) 2022-06-16 2023-12-28 株式会社日立製作所 単一細胞解析用容器及びそれを用いた単一細胞解析方法
CN115386621A (zh) * 2022-06-21 2022-11-25 厦门大学 一种测序技术中的文库制备方法
WO2025199822A1 (zh) * 2024-03-27 2025-10-02 深圳华大生命科学研究院 合成cDNA双链的方法及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014141386A1 (ja) 2013-03-12 2014-09-18 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
US20150337298A1 (en) 2014-05-23 2015-11-26 Fluidigm Corporation Haploidome determination by digitized transposons
JP2016511243A (ja) 2013-02-08 2016-04-14 テンエックス・ジェノミクス・インコーポレイテッド ポリヌクレオチドバーコード生成
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016511243A (ja) 2013-02-08 2016-04-14 テンエックス・ジェノミクス・インコーポレイテッド ポリヌクレオチドバーコード生成
WO2014141386A1 (ja) 2013-03-12 2014-09-18 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
US20150337298A1 (en) 2014-05-23 2015-11-26 Fluidigm Corporation Haploidome determination by digitized transposons
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RNA Biol.，2017年05月，vol.14, no.5，pp.637-650

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JP2019103415A (ja) 2019-06-27
WO2019116800A1 (ja) 2019-06-20

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