JP6997647B2 - Pseudo-breast cancer skin ulcer disease odor composition - Google Patents

Description

The present invention relates to a pseudo-breast cancer skin ulcer disease odor composition, a method for evaluating the strength of the breast cancer skin ulcer disease odor using the same, and a method for evaluating a deodorizing effect on the breast cancer skin ulcer disease odor.

In recent years, the number of people suffering from breast cancer has been increasing, and the characteristic point is that the morbidity rate increases from the 40s in the prime of work and that the patient has a skin ulcer accompanied by a foul odor (disease odor). For those who are socially active, the stink from skin ulcers is accompanied by not only physical pain but also mental and social pain, and the QOL (Quality of life) of breast cancer patients is lowered. Will be done. Furthermore, bad odors cause stress not only for the patients themselves but also for their families, medical staff, and long-term care workers, and their deodorization has become an important issue. On the other hand, clinical deodorization test for breast cancer skin ulcer disease odor is difficult because it may cause mental distress to the subject.

In addition, substances that cause skin ulcer disease odor derived from cancer are also being investigated. For example, in Non-Patent Document 1, a common odor component exists in skin ulcers of breast cancer and head and neck cancer, and the component is dimethyl trisulfide. Therefore, by controlling the production of dimethyl trisulfide. It is disclosed that the QOL of cancer patients can be improved. The odor of dimethyl trisulfide is generally referred to as an onion-like odor. Patent Document 1 discloses a pseudo-gynecologic cancer disease odor composition containing trimethylamine and sulfides.

Mika Shirasu et al., Biosci. Biotechnol. Biochem., Vol.73, No.9, pp2117-2120: 2009

Japanese Unexamined Patent Publication No. 2012-167957

However, neither literature has investigated the characteristics of breast cancer-derived skin ulcer disease odor. In Patent Document 1, cervical cancer, uterine body cancer, and ovarian cancer are defined as "gynecological cancer" to distinguish between the odor of breast cancer and the odor of gynecological cancer. Does not cover the odor of the disease (paragraphs [0009], [0007]). Under these circumstances, in order to improve the QOL of working breast cancer patients, the strength of the breast cancer skin ulcer disease odor and the deodorizing effect on the breast cancer skin ulcer disease odor can be more quickly, efficiently and accurately. It is necessary to evaluate, and for this purpose, it is indispensable to develop a pseudo-breast cancer skin ulcer odor composition having the characteristics of the actual breast cancer skin ulcer odor.

Therefore, the present invention relates to a pseudo-breast cancer skin ulcer disease odor composition that reproduces the characteristics of breast cancer skin ulcer disease odor.

When the present inventors investigated the odorous component generated from the affected part of the breast cancer skin ulcer, they found that the odorous component characteristic of the odor of the breast cancer skin ulcer was diacetyl, and diacetyl or a reduced form thereof was used. We have found that by combining a certain acetoin with a specific lower fatty acid in a specific ratio, it is possible to reproduce an odor very close to the actual odor of breast cancer skin ulcer disease.

The present invention contains at least the following components (A) and (B) as odor components, and the total amount of the components (A) and the component (B) is 80% by mass or more of the total odor components, and the component (A) Provided is a pseudo-breast cancer skin ulcer disease odor composition (hereinafter referred to as "pseudo-odor composition") having a mass ratio (A) / (B) with the component (B) of 1/10000 or more and 1/30 or less. It is a thing.
(A) One or two selected from diacetyl and acetoin
(B) One or more fatty acids having 4 or 5 carbon atoms

The present invention also provides a method for evaluating the intensity of a disease odor derived from a breast cancer skin ulcer using the above-mentioned pseudo-odor composition.

Further, the present invention provides a method for evaluating a deodorizing effect on a disease odor derived from a breast cancer skin ulcer using the above-mentioned pseudo odor composition.

The pseudo-odor composition of the present invention accurately reproduces the characteristics of the actual breast cancer skin ulcer disease odor and always has the same odor quality. Therefore, by using this, the strength of the breast cancer skin ulcer disease odor is increased. , And the deodorizing effect of various deodorant bases on breast cancer skin ulcer disease odor can be evaluated more quickly, efficiently and accurately, which will greatly contribute to the development of deodorizing related products for breast cancer skin ulcer disease odor. be.

The pseudo-odor composition of the present invention contains diacetyl (2,3-butandione) and acetoin (one or two selected from 3-hydroxy-2-butanone) as the component (A) as essential components. A type of diacetyl, which has a butter-like and cheese-like odor and is an odorous component that emits a stuffy odor at low concentrations. On the other hand, acetoin is a compound having a yogurt-like and butter-like scent.

The content of the component (A) in the simulated odor composition of the present invention is preferably 0.0000005% by mass or more, more preferably 0.000005% by mass or more, still more preferably 0.00005% by mass or more, and preferably 0.1% by mass. Hereinafter, it is more preferably 0.05% by mass or less, still more preferably 0.01% by mass or less.

[Component (B)]
The pseudo-odor composition of the present invention contains at least one fatty acid having 4 or 5 carbon atoms as the component (B). Examples of the fatty acid of the component (B) include butyric acid, isobutyric acid, valeric acid, isovaleric acid, pivalic acid, 2-methylbutyric acid and the like. Among them, a combination of two or more kinds including butyric acid and isovaleric acid is preferable.

The content of the component (B) in the simulated odor composition of the present invention is preferably 0.002% by mass or more, more preferably 0.02% by mass or more, still more preferably 0.8% by mass or more, and preferably 8% by mass or less. It is more preferably 7% by mass or less, still more preferably 5% by mass or less.

Further, the total amount of the component (A) and the component (B) in the simulated odor composition of the present invention is 80% by mass or more of the total odor component, but it is preferable from the viewpoint of the similarity of the breast cancer disease odor. It is 85% by mass or more, more preferably 90% by mass or more.

Further, the mass ratio (A) / (B) of the content of the component (A) to the content of the component (B) in the simulated odor composition of the present invention is based on the viewpoint of the degree of similarity with the odor of breast cancer skin ulcer. , 1/10000 or more, preferably 1/8000 or more, more preferably 1/6000 or more, and 1/30 or less, preferably 1/100 or less, more preferably 1/1000 or less. Is.

[Component (C)]
The pseudo-odor composition of the present invention further contains one or two odorous components selected from phenol and p-cresol as the component (C) from the viewpoint of improving the similarity with the odor of breast cancer skin ulcer disease. Is preferable. The content of the component (C) in the simulated odor composition of the present invention is preferably 0.00005% by mass or more, more preferably 0.0005% by mass or more, still more preferably 0.005% by mass or more, and preferably 1% by mass. Hereinafter, it is more preferably 0.5% by mass or less, still more preferably 0.1% by mass or less.

[Other ingredients]
Further, the pseudo-odor composition of the present invention preferably further contains an arbitrary odor component in addition to the above-mentioned components (A) to (C) for the purpose of adjusting the quality of odor. Specific examples thereof include aldehydes, fatty acids other than the component (B), volatile nitrogen-containing compounds such as trimethylamine, volatile sulfur-containing compounds such as dimethyl trisulfide, aromatic carboxylic acids, cyclic carboxylic acids, and ketones. Of these, trimethylamine and dimethyl trisulfide are preferable. The content of these other odorous components in the pseudo-odor composition of the present invention is preferably 0.00005% by mass or more, more preferably 0.0005% by mass or more, from the viewpoint of adjusting the quality of odor and the balance of similarity with the diseased odor. It is more preferably 0.005% by mass or more, preferably 1% by mass or less, more preferably 0.5% by mass or less, still more preferably 0.1% by mass or less.

The pseudo-odor composition of the present invention preferably contains a solvent (diluent) for the purpose of adjusting the handleability and the concentration. The diluted solution is preferably one that does not affect the odor of breast cancer, and specifically, water, ethanol, propylene glycol, dipropylene glycol, diethylphthalate, triethylcitrate, benzylbenzoate, liquid paraffin, isopropylmillistate. , Triethyl citrate, acetone and the like. The content of the diluent in the simulated odor composition of the present invention is preferably 50% by weight or more, more preferably 80% by weight or more, still more preferably 90% by weight or more.

The pseudo-odor composition of the present invention may further contain a cationic, anionic, nonionic, amphoteric or the like surfactant as long as it does not affect the disease odor of breast cancer.

[Solid support]
The pseudo-odor composition of the present invention can be used in a liquid state or by impregnating a solid carrier. The solid carrier is not particularly limited as long as it can support the pseudo-odor composition, but for example, cellulose and its derivatives, paper, non-woven fabrics, fibers, resins (polypropylene, polyethylene, polyester, nylon (registered trademark), polyurethane). , Polyvinyl, Polyvinylidene, Ethylene-polyvinyl alcohol copolymer, Polyester, Polyacrylate, Allylstyrene copolymer) and the like. The form of the solid carrier is not particularly limited, and can be used as, for example, powder, granular, sheet-like, lump-like, or the like.

〔Evaluation methods〕
Using the pseudo-odor composition of the present invention, the intensity of the odor derived from the skin ulcer of breast cancer and the deodorizing effect on the odor derived from the skin ulcer of breast cancer can be evaluated. Here, the deodorizing effect means a physical deodorizing effect or a chemical deodorizing effect using a deodorizing base or the like, and a sensory deodorizing effect using a fragrance masking agent or a modulator.

The intensity of breast cancer skin ulcer disease odor can be evaluated, for example, by sniffing and comparing the pseudo-odor composition of the present invention and the odor of a breast cancer skin ulcer disease odor sample (used absorbent article, etc.). can.

Further, in the evaluation of the deodorizing effect on the odor of breast cancer skin ulcer, for example, a deodorizing base expected to have a deodorizing effect is added to the simulated odor composition of the present invention, or the simulated odor composition and the subject are evaluated. It can be done by contacting with and sniffing the odor and comparing it with the odor of the pseudo-odor composition before the deodorant base is allowed to act. For example, a cup or the like can be used for the evaluation, and the odor is preferably evaluated by 1 to 20 specialized panelists, and is preferably evaluated on a scale of 3 to 10. Further, instead of the sensory test as described above, the deodorizing effect on the odor of breast cancer skin ulcer may be evaluated by analysis by a chemical and / or physical method such as GC-MS.

Examples 1 to 8, Comparative Examples 1 to 4
A cotton ball having a diameter of about 1 cm was placed in a 50 mL glass bottle, 100 μL of a sample mixed with the components shown in Table 1 was added dropwise thereto, and the mixture was sealed for 30 minutes. After that, three specialized panels evaluated the similarity to the odor of breast cancer skin ulcer and the strength of odor based on the evaluation criteria shown below, and made a consensus decision. The results are shown in Table 1.

<Evaluation criteria for similarity to breast cancer skin ulcer disease odor>
A: Very similar B: Very similar C: Somewhat similar D: Not very similar E: Not at all similar

<Evaluation criteria for odor strength>
5: Strong odor 4: Strong odor 3: Easily perceptible odor 2: Weak odor that tells what odor is 1: Finally perceptible odor 0: Odorless

Examples 1 to 8 in which the mass ratio (A) / (B) is in the range of 1/30 to 1/10000 resemble the odor of breast cancer skin ulcer, and among them, (A) / (B) is 1 /. Examples 2-4, which are 5000-1 / 2000, closely resembled the odor of breast cancer skin ulcers.
On the other hand, Comparative Example 1 in which the mass ratio (A) / (B) is 1/12000 and Comparative Example 4 containing only the component (B) as an odor component are odors reminiscent of rotten dairy products and dirty socks. Was strong and did not resemble or not resemble the odor of breast cancer skin ulcers. Further, Comparative Example 2 having a mass ratio (A) / (B) of 1/25 and Comparative Example 3 containing only the component (A) as an odor component have a strong odor reminiscent of a dairy product having a strong fermented odor. , Breast cancer skin ulcers did not resemble or did not resemble the odor at all.

Examples 9-10, Comparative Examples 5-6
A cotton ball having a diameter of about 1 cm was placed in a 50 mL glass bottle, 100 μL of a sample mixed with the components shown in Table 2 was added dropwise thereto, and the mixture was sealed for 30 minutes. After that, three specialized panels, one doctor and two nurses involved in the treatment of breast cancer patients evaluated the similarity to the odor of breast cancer skin ulcer and the strength of odor based on the above evaluation criteria. , Judged by consensus. The results are shown in Table 2.

Considering the threshold and vapor pressure of each compound detected by gas chromatograph mass spectrometry of absorbable articles used by patients with breast cancer skin ulcers, further, the appropriate mass ratio (A) of component (A) to component (B) ) / (B), Examples 9 and 10 were very similar to the odor of breast cancer skin ulcers.
On the other hand, Comparative Example 6 in which the compounds were mixed at the ratio of the analytical values obtained by gas chromatograph mass spectrometry did not resemble the odor of breast cancer skin ulcer at all. Comparative Example 5 in which the compound described in Non-Patent Document 1 was mixed in the same compounding ratio as in Example 9 did not resemble the odor of breast cancer skin ulcer.

Example 3a to Example 3c
As a test sample, a pseudo-odor composition (Example 3) having a mass ratio (A) / (B) of 1/3000 was used, and a solution diluted 10 to 1000 times with ion-exchanged water was prepared. After dropping 1 mL of the above solution onto a commercially available absorbent article (Koyo-made Sarasara Mini) cut into 12 cm × 15 cm, the solution was allowed to stand for 30 minutes.
The strength of the odor of these compositions was evaluated by three expert panels based on the above evaluation criteria, and was judged by consensus. The results are shown in Table 3.

By diluting the pseudo-odor composition (Example 3) having an odor intensity of 4 with ion-exchanged water, a breast cancer skin ulcer disease odor composition having an odor intensity of 1 to 3 could be prepared. .. Examples 3 and 3a to 3c are useful as pseudo-odor compositions for evaluating the deodorizing effect.

Test Example 1
As a test sample, a solution (Example 3a) in which a pseudo-odor composition (Example 3) having a mass ratio (A) / (B) of 1/3000 was further diluted 10-fold with ion-exchanged water was used, and 0.5 thereof. mL was added dropwise to a 9 cm square knitted cotton cloth.
A 0.1% by mass aqueous solution of 2-amino-2-hydroxymethyl-1,3-propanediol was prepared, and the pH was further adjusted to 7.5 with 1 mol / L hydrochloric acid to prepare a deodorant.

(Deodorant test)
Prepare two knitted cotton cloths to which the pseudo-odor composition of Example 3a is attached, spray the deodorant prepared above on one of them 15 times (0.9 g), leave it for 10 minutes, and then evaluate the strength of the odor. did.
As a result, the strength of the odor of the knitted cotton cloth sprayed with the deodorant was 3, but the strength of the odor of the knitted cotton cloth sprayed with the deodorant decreased to 2, and the skin ulcer disease of breast cancer. It was judged to be suitable as a deodorant base for odors.

Test Example 2
As a test sample, a solution (Example 3a) in which the pseudo-odor composition (Example 3) having a mass ratio (A) / (B) of 1/3000 was further diluted 10-fold with ion-exchanged water was used.
As an absorbent article for deodorization evaluation, a commercially available absorbent article (Sarasara Mini made by Koyo) cut into 12 cm x 15 cm (without activated carbon) and cut in the same manner, the non-woven fabric on the surface is peeled off, and uniform underneath. Activated carbon was sprayed on the surface (90 mg per 12 cm x 15 cm), and the non-woven fabric on the peeled surface was re-layered (with activated carbon).

(Deodorant test)
1 mL of the above solution (Example 4a) was dropped onto each of the 12 cm × 15 cm absorbent articles for deodorant evaluation with and without activated carbon, folded in half so that the dropping surface was on the inside, and left for 1 hour. The strength of the odor remaining on the dropping surface was evaluated.
As a result, the strength of the odor emitted from the absorbent article containing no activated carbon was 3, but the strength of the odor of the absorbent article containing activated carbon was reduced to 2, and the odor of breast cancer skin ulcer was eliminated. It was judged to be suitable as an odor-absorbing article.

Claims (4)

It contains at least the following components (A) and (B) as odor components, and the total amount of the components (A) and (B) is 80% by mass or more of the total odor components, and the components (A) and (B) Pseudo-breast cancer skin ulcer odor composition having a mass ratio (A) / (B) of 1/10000 or more and 1/30 or less.
(A) One or two selected from diacetyl and acetoin
(B) One or more fatty acids having 4 or 5 carbon atoms The pseudo-breast cancer skin ulcer odor composition according to claim 1, further comprising the component (C).
(C) One or two selected from phenol and p-cresol A method for evaluating the intensity of a breast cancer skin ulcer-derived disease odor using the pseudo-breast cancer skin ulcer disease odor composition according to claim 1 or 2. A method for evaluating a deodorizing effect on a breast cancer skin ulcer-derived odor using the pseudo-breast cancer skin ulcer disease odor composition according to claim 1 or 2.