JP6964034B2 - How to make amazake with high sugar content - Google Patents

How to make amazake with high sugar content Download PDF

Info

Publication number
JP6964034B2
JP6964034B2 JP2018069262A JP2018069262A JP6964034B2 JP 6964034 B2 JP6964034 B2 JP 6964034B2 JP 2018069262 A JP2018069262 A JP 2018069262A JP 2018069262 A JP2018069262 A JP 2018069262A JP 6964034 B2 JP6964034 B2 JP 6964034B2
Authority
JP
Japan
Prior art keywords
rice flour
rice
enzyme
amazake
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018069262A
Other languages
Japanese (ja)
Other versions
JP2019176825A (en
Inventor
嘉平 新井
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujicco Co Ltd
Original Assignee
Fujicco Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujicco Co Ltd filed Critical Fujicco Co Ltd
Priority to JP2018069262A priority Critical patent/JP6964034B2/en
Publication of JP2019176825A publication Critical patent/JP2019176825A/en
Application granted granted Critical
Publication of JP6964034B2 publication Critical patent/JP6964034B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Description

本発明は、高糖度の甘酒の製造方法に関するものである。 The present invention relates to a method for producing amazake having a high sugar content.

米に含まれる澱粉を米麹によって糖化させた甘酒は、健康的な甘味飲料として古くから好まれてきた。近年は精製された砂糖や人工甘味料を忌避するマクロビオティックなどの健康法において、甘味料としての需要も高まっている。 Amazake, which is made by saccharifying starch contained in rice with rice jiuqu, has long been favored as a healthy sweet drink. In recent years, demand as a sweetener has been increasing in health methods such as macrobiotics that avoid refined sugar and artificial sweeteners.

甘酒の伝統的な製法では、蒸煮した米に米麹と水を加え、米麹に含まれる酵素により米澱粉を糖化させて製造する。家庭でも作ることのできる容易な技術ではあるが、大量生産する場合には、米の水浸漬、米の蒸煮、酵素反応を実施させる保温の工程があり、各工程のための設備が必要であった。 In the traditional method of making amazake, rice jiuqu and water are added to steamed rice, and rice starch is saccharified by enzymes contained in the rice jiuqu. Although it is an easy technology that can be made at home, in the case of mass production, there are processes of soaking rice in water, steaming rice, and heat retention to carry out an enzymatic reaction, and equipment for each process is required. rice field.

特許文献1では、米粉などの澱粉を液化酵素で液化した後、糖化酵素で糖化し、得られた糖液を乳酸菌で発酵させるヨーグルト様飲料が提案されている。米ではなく米粉を使用しており、伝統的な製法と比較して、米の水浸漬・蒸煮の工程が省略されている点が優れている。しかし、糊化による増粘を避けようとすると、米澱粉の糊化が急速に進まないよう昇温速度を抑え、時間をかけて液化酵素を反応させる必要があるため、効率が悪い。 Patent Document 1 proposes a yogurt-like beverage in which starch such as rice flour is liquefied with a liquefying enzyme, then saccharified with a saccharifying enzyme, and the obtained sugar solution is fermented with lactic acid bacteria. It uses rice flour instead of rice, and is superior in that the process of soaking and steaming rice is omitted compared to the traditional manufacturing method. However, in order to avoid thickening due to gelatinization, it is necessary to suppress the rate of temperature rise so that the gelatinization of rice starch does not proceed rapidly, and to react the liquefied enzyme over a long period of time, which is inefficient.

特許第6029150号公報Japanese Patent No. 6029150

本発明者は、高糖度の甘酒を製造するべく、米粉を高濃度で使用して甘酒の製造を試みたところ、米粉と水を撹拌機付きタンク内で溶解し加熱した際に、米澱粉の糊化により米粉溶液の粘度が著しく上昇し、米粉溶液がタンク内壁面に付着するため撹拌ができなくなり、均一に加熱することが困難だった。米粉溶液に予め液化酵素を添加してから加熱しても、糊化速度が液化酵素反応速度を上回り、同様の結果となった。そのため、米粉溶液を均一に加熱しようとした場合、米粉溶液中の米粉量は、35質量%程度が上限であった。この米粉量では米粉溶液中の澱粉が少ないため、米粉溶液を糖化しても糖度35°Bx程度しか得ることができず、甘味料として他の食品と混合して使用するには甘味が不十分であった。 The present inventor attempted to produce sweet liquor using rice flour at a high concentration in order to produce sweet liquor with a high sugar content. Due to gelatinization, the viscosity of the rice flour solution increased remarkably, and the rice flour solution adhered to the inner wall surface of the tank, making it impossible to stir, and it was difficult to heat the rice flour uniformly. Even when the liquefied enzyme was added to the rice flour solution in advance and then heated, the gelatinization rate exceeded the liquefied enzyme reaction rate, and the same result was obtained. Therefore, when trying to heat the rice flour solution uniformly, the amount of rice flour in the rice flour solution was limited to about 35% by mass. With this amount of rice flour, the amount of starch in the rice flour solution is small, so even if the rice flour solution is saccharified, a sugar content of only about 35 ° Bx can be obtained, and the sweetness is insufficient to be mixed with other foods as a sweetener. Met.

このように、簡易な設備で米の代わりに米粉を使用して甘酒を製造しようとすると、伝統的な製法の甘酒と比較して、水分が多く糖度の低いものしか得ることが出来なかった。本発明は、簡易な設備で米粉溶液中の米粉濃度を上げ、高糖度の甘酒を製造する方法を提供することを目的とする。 In this way, when trying to produce amazake using rice flour instead of rice with simple equipment, only amazake with a high water content and a low sugar content could be obtained as compared with the traditional method of amazake. An object of the present invention is to provide a method for producing amazake having a high sugar content by increasing the concentration of rice flour in a rice flour solution with a simple facility.

本発明者は、上記課題を解決するため検討を重ねた結果、簡易な設備で米粉を原料とした高濃度の甘酒を製造する方法を見出した。即ち、水と、米粉と、液化酵素を含む米粉溶液を調整し、液化酵素によって米澱粉を液化する米粉液化工程(工程1)、米粉を添加する米粉添加工程(工程2)、糖化酵素と米麹を加え、糖化酵素及び米麹によりグルコース及び/又はマルトースを生成させる糖化工程(工程3)を順に含み、前記米粉液化工程における米粉の添加量が前記米粉溶液中15〜35重量%であり、且つ前記米粉添加工程における米粉の添加量が前記米粉溶液中の総量で30〜55重量%となるように調整されることを特徴とする高糖度の甘酒の製造方法である。 As a result of repeated studies to solve the above problems, the present inventor has found a method for producing high-concentration amazake using rice flour as a raw material with simple equipment. That is, a rice flour liquefaction step (step 1) in which water, rice flour, and a rice flour solution containing a liquefying enzyme are prepared and rice starch is liquefied by the liquefying enzyme, a rice flour addition step (step 2) in which rice flour is added, and a saccharifying enzyme and rice. The saccharification step (step 3) of adding koji and producing glucose and / or maltose by saccharifying enzyme and rice koji is included in order, and the amount of rice flour added in the rice flour liquefaction step is 15 to 35% by weight in the rice flour solution. Moreover, it is a method for producing sweet sake having a high sugar content, characterized in that the amount of rice flour added in the rice flour addition step is adjusted so that the total amount in the rice flour solution is 30 to 55% by weight.

本発明により、原料に米粉を使用しながら、伝統的な製法と同等かそれ以上の濃度の米澱粉を糖化させることができ、高糖度の甘酒を得ることができる。米粉を使用することにより、水浸漬・蒸煮工程を省略することができるため、伝統的製法よりも少ない設備と工程で甘酒を生産することができる。米粉の糊化による粘度上昇を抑えながら米粉濃度を上げることができるので、作業性が良好で、かつ簡易な装置で実施可能である According to the present invention, while using rice flour as a raw material, rice starch having a concentration equal to or higher than that of the traditional production method can be saccharified, and amazake having a high sugar content can be obtained. By using rice flour, the steps of soaking in water and steaming can be omitted, so amazake can be produced with fewer equipment and steps than the traditional manufacturing method. Since the rice flour concentration can be increased while suppressing the increase in viscosity due to gelatinization of rice flour, workability is good and it can be carried out with a simple device.

本発明の原料として用いる米粉は、粳米、糯米、いずれであってもよく、また精白度もいずれであってもよい。風味や色調の調整のため、穀類、芋類、豆類等の粉末を適宜混合してもよい。 The rice flour used as the raw material of the present invention may be either glutinous rice or glutinous rice, and may have any degree of whitening. In order to adjust the flavor and color tone, powders of cereals, potatoes, beans and the like may be appropriately mixed.

液化酵素はα‐アミラーゼであり、至適温度は限定するものではないが、糊化した澱粉に作用させると効果が高いことから、米澱粉の糊化温度である60〜80℃の範囲内にあることが好ましく、更に好ましくは70〜80℃の範囲内にあるものがよい。 The liquefied enzyme is α-amylase, and the optimum temperature is not limited, but since it is highly effective when acted on gelatinized starch, it is within the range of 60 to 80 ° C., which is the gelatinization temperature of rice starch. It is preferable that the temperature is in the range of 70 to 80 ° C.

工程1は、米澱粉を液化酵素により液化する、米粉液化工程である。工程1において、液化酵素は好ましくは米粉の0.001〜0.15質量%量で使用する。
工程1では、水と米粉と液化酵素を、米粉が糊化温度に達しない50℃以下の水温で、米粉が米粉溶液の15〜35質量%となる量で混合し、撹拌しながら加熱する。米澱粉の糊化温度以上かつ液化酵素の至適温度まで昇温し、酵素反応を実施させる。温度が高い方が糊化が進むため、酵素の至適温度以上、例えば90℃まで昇温してもよいが、液化酵素の至適温度付近の温度、例えば75℃を維持してもよい。
Step 1 is a rice flour liquefaction step in which rice starch is liquefied by a liquefying enzyme. In step 1, the liquefied enzyme is preferably used in an amount of 0.001 to 0.15% by mass of rice flour.
In step 1, water, rice flour, and liquefying enzyme are mixed at a water temperature of 50 ° C. or lower at which the rice flour does not reach the gelatinization temperature in an amount such that the rice flour is 15 to 35% by mass of the rice flour solution, and heated with stirring. The temperature is raised above the gelatinization temperature of rice starch and to the optimum temperature of the liquefied enzyme, and the enzyme reaction is carried out. Since gelatinization proceeds at a higher temperature, the temperature may be raised above the optimum temperature of the enzyme, for example, 90 ° C., but the temperature near the optimum temperature of the liquefied enzyme, for example, 75 ° C. may be maintained.

工程2は、工程1の実施後、米粉溶液に米粉を追加投入することで米粉濃度を上げる、米粉添加工程である。米粉溶液の温度が液化酵素の至適温度以上である場合は、至適温度、例えば75℃になるまで冷却し、米粉を投入する。投入する米粉量は、米粉溶液中において総米粉量が30〜55質量%になるよう調整する。投入する米粉の35質量%未満の水を追加投入してもよい。工程2での液化効率を高めるために、追加する水に工程1と同程度の液化酵素を溶解しておいてもよい。 Step 2 is a rice flour addition step in which the rice flour concentration is increased by adding rice flour to the rice flour solution after the implementation of step 1. When the temperature of the rice flour solution is equal to or higher than the optimum temperature of the liquefying enzyme, the rice flour is cooled to the optimum temperature, for example, 75 ° C., and the rice flour is added. The amount of rice flour to be added is adjusted so that the total amount of rice flour in the rice flour solution is 30 to 55% by mass. Water of less than 35% by mass of the rice flour to be added may be additionally added. In order to increase the liquefaction efficiency in step 2, the same level of liquefying enzyme as in step 1 may be dissolved in the added water.

米粉の追加投入後、撹拌しながら加熱し、米澱粉の糊化温度以上かつ液化酵素が失活しない温度まで昇温し、酵素反応を実施させる。ここでも酵素の至適温度以上、例えば90℃まで昇温してよく、液化酵素の至適温度付近の温度、例えば75℃を維持してもよい
工程2は、目的の米粉濃度に達するまで、投入する米粉を分割し2回以上繰り返してもよい。
After the additional rice flour is added, the rice flour is heated with stirring to raise the temperature to a temperature equal to or higher than the gelatinization temperature of the rice starch and a temperature at which the liquefied enzyme is not inactivated, and the enzyme reaction is carried out. Again, the temperature may be raised above the optimum temperature of the enzyme, for example 90 ° C., and the temperature near the optimum temperature of the liquefied enzyme, for example 75 ° C., may be maintained in step 2 until the desired rice flour concentration is reached. The rice flour to be added may be divided and repeated twice or more.

糖化酵素はグルコアミラーゼ又はβ‐アミラーゼのどちらであっても、又は両方を使用してもよい。工程3において、米麹を用いて糖化を実施するため、糖化酵素も至適温度が米麹由来の酵素の至適温度である50〜60℃付近であるものが望ましいが、限定するものではない。 The saccharifying enzyme may be either glucoamylase or β-amylase, or both may be used. In step 3, since saccharification is carried out using rice jiuqu, it is desirable, but not limited to, that the optimum temperature of the saccharifying enzyme is around 50 to 60 ° C., which is the optimum temperature of the enzyme derived from rice jiuqu. ..

工程3は、米粉溶液を糖化酵素と米麹で糖化する、糖化工程である。工程3において、糖化酵素は好ましくは米粉の0.001〜0.15質量%量、米麹は米粉の5〜50質量%で使用する。米麹は、米粒の残る状態で使用してもよいが、甘酒に米粒が残らない品質を求めるのであれば、粉末状に破砕したものを用いてもよい。 Step 3 is a saccharification step in which the rice flour solution is saccharified with a saccharifying enzyme and rice jiuqu. In step 3, the saccharifying enzyme is preferably used in an amount of 0.001 to 0.15% by mass of rice flour, and the rice jiuqu is used in an amount of 5 to 50% by mass of rice flour. Jiuqu may be used in a state where rice grains remain, but if quality is desired so that amazake does not leave rice grains, crushed rice koji may be used.

工程3は、工程2の実施後、米粉溶液の温度が米麹の至適温度以上である場合は、至適温度以下、例えば60℃になるまで冷却し、糖化酵素と米麹を添加し、至適温度付近の温度、例えば55〜60℃を維持して糖化反応を実施する。 In step 3, after the implementation of step 2, when the temperature of the rice flour solution is equal to or higher than the optimum temperature of rice jiuqu, the rice flour is cooled to the optimum temperature or lower, for example, 60 ° C., and saccharifying enzyme and rice jiuqu are added. The saccharification reaction is carried out while maintaining a temperature near the optimum temperature, for example, 55 to 60 ° C.

以下、実施例により本願発明を説明するが、本願発明は当該実施例に限定されるものではない。 Hereinafter, the present invention will be described with reference to Examples, but the present invention is not limited to the Examples.

米粉は粳米の精白米を製粉したものを用いた。液化酵素は至適温度が65〜75℃であり65〜90℃の範囲で作用するヤクルト薬品工業株式会社製「ユニアーゼBM−8」を用いた。糖化酵素はグルコアミラーゼを主成分とし、至適温度が55〜60℃であるヤクルト薬品工業株式会社製「ユニアーゼ30」を用いた。 As the rice flour, milled polished rice of Japonica rice was used. As the liquefiing enzyme, "Uniase BM-8" manufactured by Yakult Pharmaceutical Co., Ltd., which has an optimum temperature of 65 to 75 ° C and operates in the range of 65 to 90 ° C, was used. As the saccharifying enzyme, "Uniase 30" manufactured by Yakult Pharmaceutical Co., Ltd., which contains glucoamylase as a main component and has an optimum temperature of 55 to 60 ° C., was used.

蒸気加熱のできる撹拌機付タンクに常温の水54.5kgと液化酵素27gを撹拌し混合した後、40rpmで撹拌しながら米粉18kgを投入し、米粉が完全に溶解するまで10分撹拌した。以下、40rpmでの撹拌を継続しながら行った。溶解確認後加熱し、米粉溶液が90℃に達した時点で加熱を止めた。加熱開始から加熱終了までの時間は60分であった。 After stirring and mixing 54.5 kg of water at room temperature and 27 g of liquefied enzyme in a tank with a stirrer capable of steam heating, 18 kg of rice flour was added while stirring at 40 rpm, and the mixture was stirred for 10 minutes until the rice flour was completely dissolved. Hereinafter, the stirring was continued at 40 rpm. After confirming the dissolution, the rice flour was heated, and when the rice flour solution reached 90 ° C., the heating was stopped. The time from the start of heating to the end of heating was 60 minutes.

加熱後の米粉溶液が80℃になるまでタンクを水冷却し、次いで、水4.5kgと液化酵素22.5gと米粉15kgをタンクに投入し、米粉が完全に溶解するまで30分撹拌した。溶解確認後加熱し、米粉溶液が90℃以上5分間を維持し米澱粉を十分糊化させた後加熱を止め、米粉溶液が60℃になるまでタンクを水冷却した。加熱開始から加熱終了までの時間は60分であった。 The tank was water-cooled until the heated rice flour solution reached 80 ° C., then 4.5 kg of water, 22.5 g of liquefied enzyme and 15 kg of rice flour were added to the tank, and the mixture was stirred for 30 minutes until the rice flour was completely dissolved. After confirming the dissolution, the rice flour solution was kept at 90 ° C. or higher for 5 minutes to sufficiently gelatinize the rice starch, then the heating was stopped, and the tank was water-cooled until the rice flour solution reached 60 ° C. The time from the start of heating to the end of heating was 60 minutes.

次いで、米麹8.25kgと500mlの水に溶解した糖化酵素16.5gをタンクに投入し、10分間撹拌した後撹拌を止め、55℃〜60℃を6時間維持して米粉溶液を糖化させた。糖化反応中、1時間ごとに3分間40rpmで撹拌した。 糖化後85℃以上35分間加熱殺菌し、殺菌後水冷却した。 Next, 8.25 kg of rice jiuqu and 16.5 g of saccharifying enzyme dissolved in 500 ml of water were put into a tank, and after stirring for 10 minutes, the stirring was stopped and the rice flour solution was saccharified by maintaining 55 ° C. to 60 ° C. for 6 hours. rice field. During the saccharification reaction, the mixture was stirred every hour for 3 minutes at 40 rpm. After saccharification, it was sterilized by heating at 85 ° C. or higher for 35 minutes, and after sterilization, it was cooled with water.

これらの工程により、味、風味ともに良好な甘酒が得られた。甘酒の糖度は42°Bxであった。

By these steps, amazake having good taste and flavor was obtained. The sugar content of amazake was 42 ° Bx.

Claims (1)

水と、米粉と、液化酵素を含む米粉溶液を調整し、液化酵素によって米澱粉を液化する米粉液化工程(工程1)、
米粉を添加する米粉添加工程(工程2)、
糖化酵素と米麹を加え、糖化酵素及び米麹によりグルコース及び/又はマルトースを生成する糖化工程(工程3)
を順に含み、
前記米粉液化工程における米粉の添加量が前記米粉溶液中15〜35重量%であり、且つ前記米粉添加工程における米粉の添加量が前記米粉溶液中の総量で30〜55重量%となるように調整される
ことを特徴とする高糖度の甘酒の製造方法。

Rice flour liquefaction step (step 1), in which water, rice flour, and a rice flour solution containing a liquefying enzyme are prepared and rice starch is liquefied by the liquefying enzyme.
Rice flour addition step (step 2), which adds rice flour,
Saccharification step of adding saccharifying enzyme and rice jiuqu to produce glucose and / or maltose with saccharifying enzyme and rice jiuqu (step 3)
In order,
The amount of rice flour added in the rice flour liquefaction step is adjusted to be 15 to 35% by weight in the rice flour solution, and the total amount of rice flour added in the rice flour addition step is adjusted to be 30 to 55% by weight in the rice flour solution. A method for producing high-sugar sweet sake, which is characterized by being rice flour.

JP2018069262A 2018-03-30 2018-03-30 How to make amazake with high sugar content Active JP6964034B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2018069262A JP6964034B2 (en) 2018-03-30 2018-03-30 How to make amazake with high sugar content

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2018069262A JP6964034B2 (en) 2018-03-30 2018-03-30 How to make amazake with high sugar content

Publications (2)

Publication Number Publication Date
JP2019176825A JP2019176825A (en) 2019-10-17
JP6964034B2 true JP6964034B2 (en) 2021-11-10

Family

ID=68276811

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018069262A Active JP6964034B2 (en) 2018-03-30 2018-03-30 How to make amazake with high sugar content

Country Status (1)

Country Link
JP (1) JP6964034B2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7008741B2 (en) * 2020-04-02 2022-01-25 青源味噌株式会社 Manufacturing method of fermented lactic acid bacteria food
CN111518652B (en) * 2020-04-24 2022-08-30 统一企业(中国)投资有限公司昆山研究开发中心 Lees sweet wine and preparation method thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5154934A (en) * 1974-11-06 1976-05-14 Toho Shuzo Kk Shironukaamasakeno seizohoho
JPS6049782A (en) * 1983-08-31 1985-03-19 Hanamaruki Kk Preparation of ama-zake (sweet drink made from fermented rice)
JP3343627B2 (en) * 1993-08-02 2002-11-11 宝ホールディングス株式会社 Alcohol production method
JPH07147967A (en) * 1993-11-30 1995-06-13 Nagase Seikagaku Kogyo Kk Production of sake using liquefied rice for charging unrefined sake obtained by method for instantaneous and continuous liquefaction
JP2010081906A (en) * 2008-10-02 2010-04-15 Sanei Gen Ffi Inc Method for producing fermented beverage
JP2011055749A (en) * 2009-09-09 2011-03-24 Banzuiin Chobee:Kk Method for producing amazake
JP6286782B2 (en) * 2013-03-20 2018-03-07 群栄化学工業株式会社 Rice saccharified liquid, method for producing the same, and liquid food using the rice saccharified liquid
JP6029150B2 (en) * 2014-11-17 2016-11-24 有限会社ラヴィアンサンテ Method for producing starch-derived yogurt-like beverage

Also Published As

Publication number Publication date
JP2019176825A (en) 2019-10-17

Similar Documents

Publication Publication Date Title
US20200390136A1 (en) Plant-based product and process
CN104886529B (en) A kind of quickly brewing method of the high alcohol dilute soy of high salt
JP2008283922A (en) Method for producing yoghurt-like food and drink
CN110205233B (en) Raw material solid-state brewing process of vinegar and brewed vinegar
JP6964034B2 (en) How to make amazake with high sugar content
KR101568600B1 (en) Method for making red yeast rice vinegar
JP2007275017A (en) Method for producing sweet alcoholic drink made from sake lees using germinated brown rice
CN105475603A (en) Preparation method of sweet potato cerealose
CN104705751A (en) Sweet potato and corn fermented beverage and production method thereof
JP2010081906A (en) Method for producing fermented beverage
KR101763992B1 (en) Manufacturing method for functional grain syrup using fruit or vegetable
JP6388460B1 (en) Method for producing a beverage by saccharifying cereals
CN101260353A (en) Technique for brewing yellow wine
CN111631268A (en) Highland barley fermented yoghourt and preparation method thereof
CN109674002B (en) Preparation method of pure fermented rice sour soup
CN104365838A (en) Red date and rice fermented milk and making method thereof
KR102204551B1 (en) Method for producing regional alcoholic beverage using 6JUL barley grains
CN110338223A (en) A kind of Chinese prickly ash Yoghourt and preparation method thereof
JP3655880B2 (en) Method for producing rice saccharified solution
JP4051333B2 (en) Process for producing fermented food containing saccharified and germinated brown rice
JPH078256A (en) Production of wine of lotus root
JP2011062165A (en) Production method of spouted brawn rice drink
JP2009131183A (en) Lactic acid fermented food of rice, and method for producing the same
CN104473272A (en) Wolfberry-rice fermented milk and preparation method thereof
CN110616121A (en) Glutinous rice wine making method

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20201210

TRDD Decision of grant or rejection written
A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20211005

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211014

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20211018

R150 Certificate of patent or registration of utility model

Ref document number: 6964034

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150