JP6963249B2 - 菌根菌の培養培地 - Google Patents
菌根菌の培養培地 Download PDFInfo
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- JP6963249B2 JP6963249B2 JP2017070158A JP2017070158A JP6963249B2 JP 6963249 B2 JP6963249 B2 JP 6963249B2 JP 2017070158 A JP2017070158 A JP 2017070158A JP 2017070158 A JP2017070158 A JP 2017070158A JP 6963249 B2 JP6963249 B2 JP 6963249B2
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Description
オートクレーブ滅菌した改変M培地にビタミン類と100μMのミリスチン酸又はミリスチン酸カリウム、100nMのGR24(合成ストリゴラクトン)、1mg/mlのペプトン(BD Difco Bacto-Peptone)を添加し、シャーレに約25mlずつ分注した。培地上にアーバスキュラー菌根菌の胞子約100個を植菌し、28℃の暗所で6週間培養した。改変M培地並びに用いたビタミン類の組成は次のとおりである。その結果を図4、図5に示した。
MgSO4・7H2O:731mg/L、KNO3:80mg/L、KCl:65mg/L、KH2PO4:4.8mg/L、Ca(NO3)・4H2O:288mg/L、Fe(III)EDTA:8mg/L、MnCl2・4H2O:3mg/L、ZnSO4・7H2O:1.3mg/L、H3BO3:1.5mg/L、CuSO4・5H2O:65μg/L、Na2MoO4・2H2O:1.2μg/L、KI:750g/L、Sucrose:10g/L、MES(pH6.5):10mM、Phytagel:3g/L、Glycine:3μg/L
(ビタミン類の組成)
Thiamine-HCl:100μg/L、Pyridoxine-HCl:100μg/L、Nicotinic acid:500μg/L、Myo-inositol:50μg/L
Claims (8)
- 2μg/mLを超える濃度で、ミリスチン酸及び/又はパルミチン酸が添加されたか、または、2μg/mLを超える濃度で、ステアリン酸、ミリスチン酸、パルミチン酸の少なくともいずれか1種の脂肪酸を添加して、さらにパルミトレイン酸も添加されたアーバスキュラー菌根菌用の培養培地。
- 前記脂肪酸のいずれかは脂肪酸塩として添加された請求項1に記載の培養培地。
- ストリゴラクトンが添加された請求項1又は2に記載の培養培地。
- 有機性窒素源が添加された請求項1〜3のいずれか1項に記載の培養培地。
- 培地中2μg/mLを超える濃度で、ミリスチン酸及び/又はパルミチン酸を添加するか、または、2μg/mLを超える濃度で、ステアリン酸、ミリスチン酸、パルミチン酸の少なくともいずれか1種の脂肪酸を添加して、さらにパルミトレイン酸も添加して、宿主植物根の非存在下でアーバスキュラー菌根菌を培養する方法。
- 前記脂肪酸のいずれかを脂肪酸塩として添加する請求項5に記載の方法。
- さらにストリゴラクトンを添加して培養する請求項5又は6に記載の方法。
- 有機性窒素源を添加して培養する請求項5〜7のいずれか1項に記載の方法。
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JP2017070158A JP6963249B2 (ja) | 2017-03-31 | 2017-03-31 | 菌根菌の培養培地 |
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JP2018170973A JP2018170973A (ja) | 2018-11-08 |
JP6963249B2 true JP6963249B2 (ja) | 2021-11-05 |
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2020213718A1 (ja) * | 2019-04-19 | 2020-10-22 | 大学共同利用機関法人自然科学研究機構 | アーバスキュラー菌根菌の培養用培地及び培養方法 |
JP7511825B2 (ja) * | 2019-11-19 | 2024-07-08 | 国立大学法人信州大学 | アーバスキュラー菌根菌の培養方法 |
JP7307026B2 (ja) | 2020-04-01 | 2023-07-11 | トヨタ自動車株式会社 | アーバスキュラー菌根菌の共生促進剤及び共生促進方法 |
CN112544342B (zh) * | 2020-12-10 | 2022-06-17 | 广东省林业科学研究院 | 一种高效中华鹅膏菌专用培养基及其制备方法与应用 |
CN113403204B (zh) * | 2021-05-12 | 2022-10-11 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | 一种促进丛枝菌根真菌非共生产孢的方法及其应用 |
CN115340955A (zh) * | 2022-08-31 | 2022-11-15 | 华南师范大学 | 一种高效培养丛枝菌根真菌的方法 |
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