JP6954458B2 - 生体試料の中の金属タンパク質の分析方法 - Google Patents
生体試料の中の金属タンパク質の分析方法 Download PDFInfo
- Publication number
- JP6954458B2 JP6954458B2 JP2020513458A JP2020513458A JP6954458B2 JP 6954458 B2 JP6954458 B2 JP 6954458B2 JP 2020513458 A JP2020513458 A JP 2020513458A JP 2020513458 A JP2020513458 A JP 2020513458A JP 6954458 B2 JP6954458 B2 JP 6954458B2
- Authority
- JP
- Japan
- Prior art keywords
- metalloprotein
- biological sample
- analyzing
- measured
- chromatogram
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/065—Preparation using different phases to separate parts of sample
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
Description
本実施例では、FeとCoの2つの元素を測定し、移動相の濃度を100mMとする。
酢酸アンモニウム溶液を移動相としたLC−ICPMSは、知られている全ての種類の金属タンパク質を分析できる。本実施例において、その中の47種の金属元素を例に挙げて分析し測定したが、その測定可能な範囲はこれに限られていない。生体試料として試料Aを採用し、移動相の濃度を100mMとする。
本実施例では、FeとCoの2つの元素を測定する。生体試料として、それぞれ試料Aと試料Bを採用し、移動相の濃度はそれぞれ100 mM、50 mM、25 mMとする。
本実施例の使用機器は実施例1と同じである。前述したように、酢酸アンモニウム溶液を移動相とすることで、メンテナンスのための中断なく多くの検体の連続分析を実現できる。本実施例は、酢酸アンモニウム溶液を移動相とするときのICPMSのデータの精度を確認するために、フローインジェクションでICPMS標準試料を300回以上の連続測定したものである。測定結果を図50に示す。図50の測定のLC−ICPMS分析条件は以下の表4に示す。
Claims (7)
- 生体試料の中の、生体分子と金属元素が結合した複合体である金属タンパク質の分析方法において、
前処理を経た生体試料に対して、液体クロマトグラフィーにより処理し金属タンパク質を分離することと、
UV検出器で前記分離された金属タンパク質を検出することと、
前記UV検出器により前記分離された金属タンパク質を検出した後に、該金属タンパク質を誘導結合プラズマ質量分析法により分析することを含み、
そのうち、移動相として酢酸アンモニウム溶液を採用することを特徴とする生体試料の中の金属タンパク質の分析方法。 - 液体クロマトグラフィーは、サイズ排除クロマトグラフィーであることを特徴とする請求項1に記載の生体試料の中の金属タンパク質の分析方法。
- 酢酸アンモニウム溶液の濃度は25〜100mMであることを特徴とする請求項1に記載の生体試料の中の金属タンパク質の分析方法。
- 酢酸アンモニウム溶液のpH値は6〜7であることを特徴とする請求項2に記載の生体試料の中の金属タンパク質の分析方法。
- 前処理において免疫アフィニティークロマトグラフィーが用いられることを特徴とする請求項1に記載の生体試料の中の金属タンパク質の分析方法。
- 金属元素はK、P、Na、Ca、Mg、Al、As、Hg、Pb、Cd、Ti、Ag、Ba、Zn、Cr、Mn、Cu、Rb、Fe、Ge、Se、Sr、Co、Ni、Mo、Sn、Sb、Pt、Cs、U、La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Ho、Er、Tm、Yb、Lu、Y、Li、又は、Bであることを特徴とする請求項1に記載の生体試料の中の金属タンパク質の分析方法。
- 分離された金属タンパク質に対して、誘導結合プラズマ質量分析法により分析する際に、更にエレクトロスプレーイオン化質量分析法により分析することを特徴とする請求項1〜6のいずれかに記載の生体試料の中の金属タンパク質の分析方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810332262.6A CN110376293A (zh) | 2018-04-13 | 2018-04-13 | 生物样品中的金属蛋白的分析方法 |
CN201810332262.6 | 2018-04-13 | ||
PCT/JP2019/015950 WO2019198811A1 (ja) | 2018-04-13 | 2019-04-12 | 生体試料の中の金属タンパク質の分析方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2019198811A1 JPWO2019198811A1 (ja) | 2021-03-11 |
JP6954458B2 true JP6954458B2 (ja) | 2021-10-27 |
Family
ID=68164031
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020513458A Active JP6954458B2 (ja) | 2018-04-13 | 2019-04-12 | 生体試料の中の金属タンパク質の分析方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US20210215650A1 (ja) |
JP (1) | JP6954458B2 (ja) |
CN (1) | CN110376293A (ja) |
WO (1) | WO2019198811A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112710756A (zh) * | 2020-12-23 | 2021-04-27 | 广东省科学院生态环境与土壤研究所 | 液相色谱-原子荧光光谱联用测定三价锑和五价锑含量的方法 |
JPWO2023037596A1 (ja) * | 2021-09-07 | 2023-03-16 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1468438A4 (en) * | 2002-01-03 | 2007-11-21 | Univ Indiana Res & Tech Corp | SIMULTANEOUS ACQUISITION OF CHEMICAL INFORMATION |
AU2005226672A1 (en) * | 2004-03-19 | 2005-10-06 | Perkinelmer Las, Inc. | Separations platform based upon electroosmosis-driven planar chromatography |
JP4835036B2 (ja) * | 2004-05-17 | 2011-12-14 | 味の素株式会社 | 有機化合物の高感度検出方法 |
WO2009077876A2 (en) * | 2007-05-31 | 2009-06-25 | Uti Limited Partnership | Method for assessing trace element related disorders in blood plasma |
JP5248841B2 (ja) * | 2007-11-05 | 2013-07-31 | 倫明 山下 | メチル水銀結合タンパク質又はメチル水銀結合ポリペプチド及びこれらの分析法 |
CN103792315B (zh) * | 2014-01-23 | 2015-07-08 | 中国计量科学研究院 | 一种人血清蛋白无机质谱联用技术的定量方法 |
-
2018
- 2018-04-13 CN CN201810332262.6A patent/CN110376293A/zh active Pending
-
2019
- 2019-04-12 JP JP2020513458A patent/JP6954458B2/ja active Active
- 2019-04-12 US US17/047,216 patent/US20210215650A1/en active Pending
- 2019-04-12 WO PCT/JP2019/015950 patent/WO2019198811A1/ja active Application Filing
Also Published As
Publication number | Publication date |
---|---|
US20210215650A1 (en) | 2021-07-15 |
WO2019198811A1 (ja) | 2019-10-17 |
JPWO2019198811A1 (ja) | 2021-03-11 |
CN110376293A (zh) | 2019-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Niedzielski et al. | A new tool for inorganic nitrogen speciation study: Simultaneous determination of ammonium ion, nitrite and nitrate by ion chromatography with post-column ammonium derivatization by Nessler reagent and diode-array detection in rain water samples | |
Schettgen et al. | Simultaneous determination of mercapturic acids derived from ethylene oxide (HEMA), propylene oxide (2‐HPMA), acrolein (3‐HPMA), acrylamide (AAMA) and N, N‐dimethylformamide (AMCC) in human urine using liquid chromatography/tandem mass spectrometry | |
Xuan et al. | Separation and identification of phytosiderophores and their metal complexes in plants by zwitterionic hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry | |
US8212207B2 (en) | Method of detecting filter extractables in biopharmaceutical products by liquid chromatography-mass spectrometry | |
JP6954458B2 (ja) | 生体試料の中の金属タンパク質の分析方法 | |
Lambertsson et al. | Validation of a simplified field-adapted procedure for routine determinations of methyl mercury at trace levels in natural water samples using species-specific isotope dilution mass spectrometry | |
AU2008223791B2 (en) | Method for the standardization of the concentration of analytes in a urine sample | |
Wolle et al. | Method development for the redox speciation analysis of iron by ion chromatography–inductively coupled plasma mass spectrometry and carryover assessment using isotopically labeled analyte analogues | |
EP2508880B1 (en) | System for diversion of a liquid chromatography eluant | |
Ferrarello et al. | Multi-elemental speciation studies of trace elements associated with metallothionein-like proteins in mussels by liquid chromatography with inductively coupled plasma time-of-flight mass spectrometric detection | |
Marchante-Gayón et al. | Multielemental trace analysis of biological materials using double focusing inductively coupled plasma mass spectrometry detection | |
Michalke | Quality control and reference materials in speciation analysis | |
CN102539558A (zh) | 一种卷烟主流烟气中硫化氢的测定方法 | |
Quiroz | Speciation analysis in chemistry | |
Beer et al. | Plasma biomarker discovery using 3D protein profiling coupled with label-free quantitation | |
Shi et al. | Investigation of mercury-containing proteins by enriched stable isotopic tracer and size-exclusion chromatography hyphenated to inductively coupled plasma-isotope dilution mass spectrometry | |
Farthing et al. | Liquid chromatography method for determination of bivalirudin in human plasma and urine using automated ortho-phthalaldehyde derivatization and fluorescence detection | |
Muse et al. | An overview of capillary electrophoresis in element speciation analysis of the environment | |
Liang et al. | Direct amino acid analysis method for speciation of selenoamino acids using high-performance anion-exchange chromatography coupled with integrated pulsed amperometric detection | |
JP6315430B2 (ja) | 対象物質の分析方法及び分析装置 | |
Rodríguez‐Gonzalo et al. | Automated sample treatment with the injection of large sample volumes for the determination of contaminants and metabolites in urine | |
JP3413654B2 (ja) | アルミニウム測定方法 | |
Um et al. | Determination of the active metabolites of sibutramine in rat serum using column‐switching HPLC | |
Renoe et al. | Use of a flow-injection sample manipulator as an interface between a" high-performance" liquid chromatograph and an atomic absorption spectrophotometer. | |
Charde et al. | Bioanalytical method development and validation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200831 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200831 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210831 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210913 |
|
R151 | Written notification of patent or utility model registration |
Ref document number: 6954458 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R151 |
|
R154 | Certificate of patent or utility model (reissue) |
Free format text: JAPANESE INTERMEDIATE CODE: R154 |