JP6873530B2 - 自己組織化合成タンパク質(Self−Assembling Synthetic Proteins) - Google Patents
自己組織化合成タンパク質(Self−Assembling Synthetic Proteins) Download PDFInfo
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- JP6873530B2 JP6873530B2 JP2015562395A JP2015562395A JP6873530B2 JP 6873530 B2 JP6873530 B2 JP 6873530B2 JP 2015562395 A JP2015562395 A JP 2015562395A JP 2015562395 A JP2015562395 A JP 2015562395A JP 6873530 B2 JP6873530 B2 JP 6873530B2
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- vegf
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Description
[関連出願の相互参照]
本出願は、2013年3月15日付で出願された発明の名称「合成自己組織化免疫原性タンパク質」の米国特許出願番号第61/791,268号に対する優先権を主張し、本明細書はその全体を参照文献として含む。
FTDIITDICGEYHNTQIHTLNDKILSYTESLVGKREIILVNFKGGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLSNSKIEKLCVWNNKTPHSIAAISMVR(配列番号1)
また別の例示的な実施形態において、組換え合成タンパク質は、リンカー配列又はスペーサー配列を含むことで、合成担体の一末端又は両末端に位置する可変配列が合成担体から分離し、かかる方式で、数個の組換えタンパク質からの合成担体成分同士を結合できるようにする。成長因子に付着するリンカー配列を有する組換え合成タンパク質は、下記の配列と実質的に類似する。
ALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCSGGSGGTSGGGGGSGFTDIITDICGEYHNTQIHTLNDKILSYTESLVGKREIILVNFKGGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLSNSKIEKLCVWNNKTPHSIAAISMVR(配列番号2)
また別の例示的な実施形態において、組換え合成タンパク質は、安定した五量体と環状に結合する。他の多量体集合体としては、例えば、二量体、三量体、四量体及びさらに大きな多量体が挙げられるが、これらに限定されず、また、本発明の範囲内で予想される。リンカー配列は多様であり得、少なくとも可変性抗原決定基の配列が合成担体ドメインの結合により多量体の集合を立体的に抑制することを防止する程度に十分な長さでなければならない。本発明の範囲内でリンカー又はスペーサーは、安定した五量体の形成を可能にする柔軟性を有し得ると考えられる。
また別の実施形態において、可変性抗原決定基は、細菌性、ウイルス性、真菌性、又はその他の病原体に由来する。
所定の例示的な実施形態において、薬学的組成物は、本開示による組換えタンパク質だけでなく、本願に規定されたようなTLRアゴニスト、コアジュバント(例えば、サイトカイン、イミダゾキノリンの免疫反応調節物質及び/又はdSLIMを含む)等から選択される1つ以上の成分をさらに含んでもよいワクチン組成物及び/又は薬学的に許容可能な担体、賦形剤又は希釈剤と組み合わせた組換え発現構造体である。
選別可能な表現型及びコード遺伝子型の多様なクローン集団間の物理的連結を含む、突然変異ライブラリーを生成して所望の特徴を有する突然変異をスクリーニングできるシステムが必要であった。かかるシステムは、特に、抗体工学分野において非常に効果的に採用された確立した技術を利用して開発された。
突然変異ポリペプチドをコードするクローンのライブラリーを炭水化物結合の自己組織化タンパク質ドメインに由来する遺伝的鋳型から生成した。本実施例において、タンパク質ドメインは、細菌性ホロトキシンのA1B5群から選択した。タンパク質データベース(Protein Data Base)等の公開されたデータバース及び科学文献から得られた構造及び機能情報は、安定したホモ五量体を形成したり、多くの哺乳類細胞型の表面上で発見される特異的炭水化物含有部位と相互作用する鋳型タンパク質ドメインの能力に関連していることが潜在的に考慮、又は証明された領域及び/又は特異的残基を識別するのに用いられた。かかる領域/残基は、後続の突然変異の誘発及び突然変異のスクリーニング段階から除かれた。
ライブラリーから突然変異体の選別を行うために、予想される多様性の表示を100〜1000倍含むのに十分な細胞が含まれ、また、OD600が0.1の領域内にあることを保障するのに十分な体積が含まれるよう、(ライブラリーの)培養物を液体培地(2×TY、100μg/mlのアンピシリン、1%のグルコース)に接種した。その後、培養物を約37℃でOD600が0.4〜0.6(即ち、対数期増殖)に至るまで(振とうしながら)培養した。その後、M13 K07のヘルパーファージを細菌細胞当たり(0.1のOD600は、8.0×108細胞/mlを取る)、〜20ヘルパーファージの割合で添加して培養物を感染させた。約30分間の静的培養後、さらに、約30分間振とうしながら細胞を培養した。カナマイシンを50μg/ml添加し、その培養物を30℃で振とうしながら一晩培養した。
突然変異が収容する最大残基数の容量を評価するために、相違する鋳型の残基又は領域を各々標的とする数個の相違するライブラリーを構成してスクリーニングした。その結果、多数のクローンが固定化炭水化物の誘導体に結合し、大半が五量体を形成する所望の選別基準を示すということが確認されたが、図3aに図示しているように、各々は、多数の特有の突然変異/相違点を含んでいた。潜在的に改善された特性を有するクローンを生成するためには、これらの多様な配列を少数のクローンに組み合わせることが必要であった。
天然の完全に機能的な形態において、グループ名「A1B5」と記載された細菌性ホロトキシンは、5つのBサブユニット及び1つのAサブユニットを含む。Bサブユニットは、中央の「ポア」を有する五量体環に組み込む。図5に図示しているように、1つのAサブユニットは前記環の頂点に位置し、ボイド内に挿入されている。Aサブユニット自体は、2つの別個のドメインを含む。ホロトキシンが細胞内へ転座するときに放出されるA1ドメインは、タンパク質複合体の毒性に関する酵素活性を含む。これは、A2ドメインによりホロトキシンの一部として保持される。これは、A1ドメインがN末端に位置し、C末端が5つのBサブユニットにより形成されたポアに突出するα−らせん構造を有する。A2のC末端の一部の残基は、Bサブユニットの残基と相互作用を形成する。BサブユニットとA2ドメインの特定の残基間の相互作用が確認されているように(Zhang R.G等(1995年))、Aサブユニット、具体的には、A2ドメインの存在は、五量体のBサブユニット環の安定化に寄与するということが提示されるのは妥当である。特定の理論に何ら限られることなく、2つのAサブユニットのドメイン間の構造的及び機能的な差により、A2ドメイン単独がBサブユニットの五量体に対してかかる安定化効果を付与すると考えられる。同様に、BサブユニットはAサブユニットの完全な不在時に五量体環を形成できることが知られているように、A2ドメインと連合し得るので、Bサブユニットと共発現するか、又は外部から添加されることでBサブユニットの五量体に安定化効果を発揮すると推定される。
Claims (23)
- 安定したホモ多量体に組み込まれる単量体ポリペプチド配列を含むことを特徴とする組換え合成タンパク質であって、
ここにおいて単量体ポリペプチド配列が、コレラ毒素B(CTB)サブユニットに由来する細菌性ホロトキシンのA1B5群の合成Bサブユニットであって、ここにおいてCTBサブユニットはミスセンス突然変異T1F、P2T、Q3D、N4I、M37I、A38I、I39L、I40V、T41N、T78S、E79N、A80S、A95S、A102VおよびN103Rを含み、ここにおいて前記ミスセンス突然変異は野生型CTB配列TPQNITDLCAEYHNTQIHTLNDKIFSYTESLAGKREMAIITFKNGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMANに関連して番号が付けられており、
ペプチドスペーサー、および
IGF−1、IGF−2、FGF1、FGF2、TGF−α、TGF−β、VEGF−A、VEGF−B、VEGF−C、VEGF−D、PDGF、NGF、EGF、HGF、BMP、PDL1、IL−1、IL−2、IL−3、IL−4、IL−5およびIL−6からなる群から選ばれる成長因子又はその一部を含むポリペプチド
を含み、ここにおいて、前記ポリペプチドがペプチドスペーサーにより合成Bサブユニットから分離される、組換え合成タンパク質。 - 前記CTBのBサブユニットは、以下のミスセンス突然変異L8I、A10G、F25L、A32V、N44GおよびV82Iをさらに含み、ここにおいて前記ミスセンス突然変異は野生型CTB配列TPQNITDLCAEYHNTQIHTLNDKIFSYTESLAGKREMAIITFKNGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMANに関連して番号が付けられている、請求項1に記載の組換え合成タンパク質。
- 前記CTBのBサブユニットは配列番号1で表される、請求項2に記載の組換え合成タンパク質。
- 前記成長因子又はその一部が形質転換成長因子(TGF)である、請求項1に記載の組換え合成タンパク質。
- 前記成長因子又はその一部が上皮成長因子(EGF)である、請求項1に記載の組換え合成タンパク質。
- 前記ポリペプチドは、少なくとも2つの相違する成長因子又はその一部を含むことを特徴とする請求項1に記載の組換え合成タンパク質。
- 前記ポリペプチドは、1つ以上の成長因子の全長若しくはその一部を単一ドメインとして含むか、又は2つ以上の多重反復体として含むことを特徴とする請求項1に記載の組換え合成タンパク質。
- 前記ペプチドスペーサーは、SSG、SSGGG、SGG、GGSGG、GGGGS、SSGGGSGGSSG、GGSGGTSGGGSG、SGGTSGGGGSGG、GGSGGTSGGGGSGG、SSGGGSGGSSG、SSGGGGSGGGSSG、SSGGGSGGSSGGG、及びSSGGGGSGGGSSGGGからなる群から選ばれる、請求項1に記載の組換え合成タンパク質。
- 前記ペプチドスペーサーは、1つ以上の宿主T−細胞のエピトープを含むことを特徴とする請求項1に記載の組換え合成タンパク質。
- 請求項1に記載の組換え合成タンパク質から多量体を構築して、多価タンパク質を形成することを含むことを特徴とする多価タンパク質の製造方法。
- 請求項1に記載の組換え合成タンパク質をともに混合して、多価ワクチン製剤を調製することを含むことを特徴とするワクチン製剤の製造方法。
- コレラ毒素B(CTB)サブユニットに由来する細菌性ホロトキシンのA1B5群の合成Bサブユニットであって、ここにおいてCTBサブユニットはミスセンス突然変異T1F、P2T、Q3D、N4I、M37I、A38I、I39L、I40V、T41N、T78S、E79N、A80S、A95S、A102VおよびN103Rを含み、ここにおいて前記ミスセンス突然変異は野生型CTB配列TPQNITDLCAEYHNTQIHTLNDKIFSYTESLAGKREMAIITFKNGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMANに関連して番号が付けられており、
ペプチドスペーサー、および
腫瘍抗原の少なくとも1部を含むポリペプチド
を含み、
ここにおいて前記ポリペプチドがペプチドスペーサーにより合成Bサブユニットから分離される、組換えタンパク質。 - 前記CTBのBサブユニットは、以下のミスセンス突然変異L8I、A10G、F25L、A32V、N44GおよびV82Iをさらに含み、ここにおいて前記ミスセンス突然変異は野生型CTB配列TPQNITDLCAEYHNTQIHTLNDKIFSYTESLAGKREMAIITFKNGATFQVEVPGSQHIDSQKKAIERMKDTLRIAYLTEAKVEKLCVWNNKTPHAIAAISMANに関連して番号が付けられている、請求項12に記載の組換えタンパク質。
- 前記Bサブユニットは配列番号1で表される、請求項13に記載の組換えタンパク質。
- 前記腫瘍抗原の少なくとも一部は、1つ以上の腫瘍抗原の全長又は一部を含むことを特徴とする請求項12に記載の組換えタンパク質。
- 前記腫瘍抗原の少なくとも一部は、1つ以上の腫瘍抗原の全長又は一部を単一エピトープ又は多重反復体として含むことを特徴とする請求項12に記載の組換えタンパク質。
- 成長因子の少なくとも一部を含む第2のポリペプチドをさらに含む、請求項12に記載の組換えタンパク質。
- 前記第2のポリペプチドは、IGF−1、IGF−2、FGF1、FGF2、TGF−α、TGF−β、VEGF−A、VEGF−B、VEGF−C、VEGF−D、PDGF、NGF、EGF、HGF、BMP、IL−1、IL−2、IL−3、IL−4、IL−5およびIL−6からなる群より選択される1以上の成長因子のうち1つ以上の全長又はその一部を含むことを特徴とする請求項17に記載の組換えタンパク質。
- 前記第2のポリペプチドは、少なくとも2つの相違する成長因子の全長又はその一部を含むことを特徴とする請求項18に記載の組換えタンパク質。
- 前記第2のポリペプチドは、1つ以上の成長因子の全長若しくはその一部を単一エピトープとして含むか、又は2つ以上のタンデム反復体として含むことを特徴とする請求項18に記載の組換えタンパク質。
- 前記第2のポリペプチドは、受容体の少なくとも一部、または、2つ〜4つの相違する受容体の全長又は一部をさらに含む、請求項17に記載の組換えタンパク質。
- IGF−1、IGF−2、FGF1、FGF2、TGF−α、TGF−β、VEGF−A、VEGF−B、VEGF−C、VEGF−D、PDGF、NGF、EGF、HGF、BMP、IL−1、IL−2、IL−3、IL−4、IL−5およびIL−6からなる群より選択される成長因子のうち1つ以上の全長又はその一部を含む第3のポリペプチドをさらに含む、請求項17に記載の組換えタンパク質。
- 前記第3のポリペプチドは、1つ以上の成長因子の全長若しくはその一部を単一エピトープとして含むか、又は2つ以上のタンデム反復体として含むことを特徴とする請求項22に記載の組換えタンパク質。
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