JP6853632B2 - Salty enhancer - Google Patents

Salty enhancer Download PDF

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JP6853632B2
JP6853632B2 JP2016165163A JP2016165163A JP6853632B2 JP 6853632 B2 JP6853632 B2 JP 6853632B2 JP 2016165163 A JP2016165163 A JP 2016165163A JP 2016165163 A JP2016165163 A JP 2016165163A JP 6853632 B2 JP6853632 B2 JP 6853632B2
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salty taste
lactic acid
salty
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宏輔 菊地
宏輔 菊地
茂木 和之
和之 茂木
根津 亨
亨 根津
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Adeka Corp
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Description

本発明は、少量添加で、飲食品の風味や味質を損ねることなく塩味を強化することが可能な塩味強化剤、及び該塩味強化剤を用いた飲食品に関する。 The present invention relates to a salty taste enhancer capable of enhancing saltiness without impairing the flavor and taste quality of foods and drinks by adding a small amount, and foods and drinks using the salty taste enhancer.

食塩、即ち塩化ナトリウムを用いて調味される塩味は、飲食品の風味として極めて重要である。しかし、健康上の問題から、近年ではナトリウム含有量を低減させた減塩飲食品が好まれる傾向にある。 The salty taste seasoned with salt, that is, sodium chloride, is extremely important as the flavor of food and drink. However, due to health problems, in recent years, low-salt foods and drinks with a reduced sodium content have tended to be preferred.

一方で、飲食品中の塩化ナトリウム含量を単に減じただけでは、ナトリウム摂取量は低減されるものの、同時に塩味強度や飲食品の風味や味質が低下する。その為、飲食品の風味を維持したまま、塩化ナトリウム含量を低減させることのできる方法が検討されてきた。この方法を大別すると、(1)塩化カリウムや有機酸金属塩等の塩化ナトリウム代替品を使用する方法と、(2)塩味強化剤を使用する方法の2つが挙げられる。 On the other hand, simply reducing the sodium chloride content in foods and drinks reduces the sodium intake, but at the same time reduces the saltiness intensity and the flavor and taste of the foods and drinks. Therefore, a method capable of reducing the sodium chloride content while maintaining the flavor of food and drink has been studied. This method can be roughly classified into two methods: (1) a method using a sodium chloride substitute such as potassium chloride or an organic acid metal salt, and (2) a method using a salty taste enhancer.

塩味強化剤とは、それ自体は塩味を示さない又は極薄い塩味であるが、塩化ナトリウムに極少量添加することで、塩化ナトリウムの塩味を強く感じさせる効果を示すものであり、少ない塩化ナトリウム含量の飲食品であっても、より塩化ナトリウム含量の高い飲食品と同等の塩味をもたせることができるというものである。つまり、塩味強化剤を使用する方法は、塩味強化剤を添加し、塩化ナトリウムが持つ塩味を喫食時に強く感じさせる方法、即ち、含有される塩化ナトリウム量あたりの塩味強度を塩味強化剤によって増大させ、飲食品中の塩化ナトリウムの含有量を低減させる方法である。 The salty taste enhancer is not salty or has a very light salty taste by itself, but when added in a very small amount to sodium chloride, it has an effect of making the salty taste of sodium chloride strongly felt, and has a small sodium chloride content. Even foods and drinks of the above can have the same salty taste as foods and drinks having a higher sodium chloride content. That is, the method of using the salty taste enhancer is a method of adding a salty taste enhancer to make the salty taste of sodium chloride strongly felt at the time of eating, that is, increasing the salty taste intensity per amount of sodium chloride contained by the salty taste enhancer. , A method of reducing the content of sodium chloride in food and drink.

この方法は、塩化カリウムや有機酸金属塩等の塩化ナトリウム代替品を使用する方法と異なり、大きな風味や味質の変化を伴わないことから、広く検討されてきた。例えば、特定の乳清ミネラル(特許文献1、特許文献2)、フルフリルアルコール(特許文献3)、酸性アミノ酸、塩基性アミノ酸及びコハク酸又はその塩(特許文献4)、乳酸ナトリウム及び/又は乳酸カリウム(特許文献5)等が、これまで塩味強化剤として検討されてきた。 This method has been widely studied because it does not involve a large change in flavor or taste, unlike the method using a sodium chloride substitute such as potassium chloride or an organic acid metal salt. For example, specific lactate minerals (Patent Document 1, Patent Document 2), furfuryl alcohol (Patent Document 3), acidic amino acids, basic amino acids and succinic acid or salts thereof (Patent Document 4), sodium lactate and / or lactic acid. Potassium (Patent Document 5) and the like have been studied as saltiness enhancers.

しかし、これらの塩味強化剤を用いた方法では、塩味強化の程度が小さかったり、十分な塩味強化効果が得られるだけの量を添加すると飲食品自体が有していない風味を付与してしまうおそれがあった。また、通常これら塩味強化剤だけでは、ナトリウム摂取量の低減、所謂「減塩効果」が乏しくなり易く、結果的に塩化カリウム等の塩化ナトリウム代替品を用いる方法と併用せざるを得ないため、塩化カリウム等が有する異味が飲食品の風味を損ねることが多かった。 However, in the method using these salty taste enhancers, if the degree of salty taste enhancement is small or if an amount sufficient to obtain a sufficient salty taste enhancing effect is added, a flavor that the food or drink itself does not have may be imparted. was there. In addition, these salty taste enhancers usually tend to reduce the sodium intake, that is, the so-called "salt-reducing effect", and as a result, they have to be used in combination with a method using a sodium chloride substitute such as potassium chloride. The off-flavor of potassium chloride and the like often impaired the flavor of food and drink.

特開2008−054662号公報Japanese Unexamined Patent Publication No. 2008-054662 特開2008−054665号公報Japanese Unexamined Patent Publication No. 2008-054665 特開2012−170393号公報Japanese Unexamined Patent Publication No. 2012-170393 特開2002−345430号公報JP-A-2002-345430 特開2008−054661号公報Japanese Unexamined Patent Publication No. 2008-054661

したがって、本発明の目的は、ナトリウム含有量が低減されている飲食品でありながら、低減する前と同等の塩味を呈することができるように、飲食品の塩味を強化することのできる塩味強化剤を提供することにある。 Therefore, an object of the present invention is a salty taste enhancer capable of enhancing the salty taste of a food or drink so that the food or drink has a reduced sodium content but can exhibit the same salty taste as before the reduction. Is to provide.

本発明者らは、鋭意検討を行った結果、特定の乳酸発酵物を有効成分とする塩味強化剤を使用することにより、異味や雑味を付与することなく、塩味の強度や発現パターンが通常の塩分含量の飲食品と同様でありながら、飲食品のナトリウム含有量を低減することが可能であることを知見した。
また、本発明者らは、上記乳酸発酵物を乳清ミネラルやエキス類と併用すると、飲食品中の塩化ナトリウムを塩化カリウムや有機酸金属塩等の塩化ナトリウム代替品で置換して更にナトリウム含有量を低減させた場合においても、塩味の強度や発現パターン、呈味を通常の塩分含量の飲食品と同様のものとすることが可能であることを知見した。
As a result of diligent studies, the present inventors have conducted a diligent study, and by using a salty taste enhancer containing a specific lactic acid fermented product as an active ingredient, the strength and expression pattern of salty taste are usually obtained without imparting an unpleasant taste or unpleasant taste. It was found that it is possible to reduce the sodium content of foods and drinks while having the same salt content as foods and drinks.
In addition, when the above lactic acid fermented product is used in combination with milky minerals and extracts, the present inventors replace sodium chloride in foods and drinks with a sodium chloride substitute such as potassium chloride or an organic acid metal salt to further contain sodium chloride. It was found that even when the amount is reduced, the intensity of saltiness, the expression pattern, and the taste can be made similar to those of foods and drinks having a normal salt content.

本発明は、上記知見に基づいて為されたものであり、乳原料を含む水中油型乳化物を基質とした乳酸発酵物を有効成分とする塩味強化剤、及び該塩味強化剤を用いた飲食品を提供するものである。 The present invention has been made based on the above findings, and is a salty taste enhancer containing a lactic acid fermented product containing an oil-in-water emulsion containing a dairy raw material as a substrate as an active ingredient, and food and drink using the salty taste enhancer. It provides goods.

本発明の塩味強化剤を使用することで、塩化ナトリウム含量が削減されているにもかかわらず、飲食品の風味や味質を損なわずに、塩味の強度や発現パターンを通常の塩分含量の飲食品と同様とすることができるため、ナトリウム摂取量を低減することができる。 By using the salty taste enhancer of the present invention, although the sodium chloride content is reduced, the salty taste intensity and expression pattern can be changed to the normal salty content of food and drink without impairing the flavor and taste quality of the food and drink. Since it can be the same as the product, the sodium intake can be reduced.

以下、本発明について好ましい実施態様に基づいて、詳述する。
本発明の塩味強化剤は、乳原料を含む水中油型乳化物を基質とした乳酸発酵物を有効成分として含有することを特徴とし、塩味剤(飲食品中に塩味付与を目的として使用される塩味物質)が飲食品に付与する塩味を増強することができる。
Hereinafter, the present invention will be described in detail based on preferred embodiments.
The salty taste enhancer of the present invention is characterized by containing a lactic acid fermented product containing an oil-in-water emulsion containing a dairy raw material as a substrate as an active ingredient, and is used for the purpose of imparting a salty taste to foods and drinks. The salty substance) can enhance the salty taste given to foods and drinks.

<乳酸発酵物>
まず、本発明の有効成分である乳酸発酵物について述べる。
乳酸発酵物とは、乳酸菌が資化可能な基質を乳酸発酵して得られた風味素材であるが、本発明では、その基質として乳原料を含む水中油型乳化物を使用することにより、少量添加で、飲食品の風味や味質を損ねることなく塩味を強化することが可能となるものである。
<Lactic acid fermented product>
First, the lactic acid fermented product which is the active ingredient of the present invention will be described.
The lactic acid fermented product is a flavor material obtained by lactic acid fermentation of a substrate that can be assimilated by lactic acid bacteria. In the present invention, a small amount of an oil-in-water emulsion containing a dairy raw material is used as the substrate. The addition makes it possible to enhance the salty taste without impairing the flavor and taste of food and drink.

上記乳原料としては、牛乳、濃縮乳、練乳、ホエイ、クリーム、バター、バタークリーム、クリームチーズ、ナチュラルチーズ、プロセスチーズ、バターオイル、乳脂分解物等の乳や乳製品をはじめ、脱脂粉乳、全粉乳、ホエイパウダー、トータルミルクプロテイン等の粉乳類や、脱脂乳、脱脂濃縮乳等の乳糖を含有する乳製品も使用可能である。 The above dairy ingredients include milk, concentrated milk, condensed milk, whey, cream, butter, butter cream, cream cheese, natural cheese, processed cheese, butter oil, milk and dairy products such as milk fat decomposition products, skim milk powder, and all. Milk powders such as powdered milk, whey powder, and total milk protein, and dairy products containing lactose such as skim milk and skim concentrated milk can also be used.

良好な風味の乳酸発酵物を安定して製造可能な点で、上記水中油型乳化物中の上記乳原料の含量は、無脂乳固形分として2〜50質量%であることが好ましく、5〜20質量%であることが更に好ましい。
尚、無脂乳固形分とは、乳や乳製品等の乳原料における、油脂以外の固形分のことであり、その主体は蛋白質と炭水化物である。上記乳原料に含まれる純油脂分は、後述する油脂の含量に含めるものとする。
The content of the milk raw material in the oil-in-water emulsion is preferably 2 to 50% by mass as the non-fat milk solid content in that a lactic acid fermented product having a good flavor can be stably produced. It is more preferably ~ 20% by mass.
The non-fat milk solids are solids other than fats and oils in dairy raw materials such as milk and dairy products, and their main components are proteins and carbohydrates. The pure fats and oils contained in the dairy raw material shall be included in the fats and oils content described later.

水中油型乳化物とするために使用する油脂としては、大豆油、菜種油、コーン油、綿実油、オリーブ油、落花生油、米油、べに花油、ひまわり油、パーム油、パーム核油、ヤシ油、サル脂、マンゴ脂、乳脂、牛脂、豚脂、カカオ脂、魚油、鯨油等の各種植物油脂及び動物油脂、大豆由来、向日葵由来、乳由来、卵由来のレシチン及びリン脂質、並びにこれらの油脂に水素添加、分別、エステル交換、酵素分解、化学的分解等の物理的又は化学的処理の1種又は2種以上の処理を施した油脂を、単独で又は2種以上を組み合わせて用いることができる。 Oils and fats used to make oil-in-water emulsions include soybean oil, rapeseed oil, corn oil, cottonseed oil, olive oil, peanut oil, rice oil, beni flower oil, sunflower oil, palm oil, palm kernel oil, palm oil, and monkeys. Various vegetable oils and fats such as fat, mango fat, milk fat, beef fat, pork fat, coconut fat, fish oil, whale oil, soybean-derived, sunflower-derived, milk-derived, egg-derived lecithin and phospholipids, and hydrogen in these fats and oils. Oils and fats that have been subjected to one or more physical or chemical treatments such as addition, separation, ester exchange, enzymatic decomposition, and chemical decomposition can be used alone or in combination of two or more.

水中油型乳化物中の油脂含量は、好ましくは1.5〜50質量%であり、より好ましくは1.5〜30質量%であり、最も好ましくは2〜20質量%である。
油脂含量が1.5質量%未満の場合、優れた塩味強化効果が得られ難いことに加え、乳酸発酵による生成物の保持力が弱くなってしまうため塩味強化剤の安定性も悪化してしまう恐れがある。また油脂含量が50質量%超の場合には、本発明の有効成分である乳酸発酵物の基質となる水中油型乳化物中に、十分に無脂乳固形分を含有させることができないため、油中水型乳化物となってしまうおそれがあり、また、水中油型乳化物の乳化安定性が乏しくなり、乳酸発酵の進行が遅れる場合がある。
尚、該油脂含量は、上記油脂、乳原料中の純油脂分に加え、後述するその他の原材料に由来する純油脂分の合計量として算出するものとする。
The oil and fat content in the oil-in-water emulsion is preferably 1.5 to 50% by mass, more preferably 1.5 to 30% by mass, and most preferably 2 to 20% by mass.
When the fat content is less than 1.5% by mass, it is difficult to obtain an excellent salty taste strengthening effect, and the retention of products by lactic acid fermentation is weakened, so that the stability of the salty taste strengthening agent is also deteriorated. There is a fear. Further, when the fat content exceeds 50% by mass, the non-fat milk solid content cannot be sufficiently contained in the oil-in-water emulsion which is the substrate of the lactic acid fermentation product which is the active ingredient of the present invention. There is a risk of becoming a water-in-oil emulsion, and the emulsion stability of the oil-in-water emulsion may be poor, which may delay the progress of lactic acid fermentation.
The fats and oils content shall be calculated as the total amount of the pure fats and oils derived from the above fats and oils and the pure fats and oils in the dairy raw materials and other raw materials described later.

本発明においては、上記油脂の中でも、特に塩味強化効果が高い塩味強化剤が得られる点から乳脂を用いることが好ましい。乳脂を使用する場合、乳脂そのものを使用することができるほか、上記乳原料として、牛乳、濃縮乳、練乳、クリーム、バター、バタークリーム、クリームチーズ、ナチュラルチーズ、プロセスチーズ等の乳脂を含有する乳や乳製品を使用することによって、乳脂を使用することもでき、これらの乳や乳製品は、単独で又は2種以上を組合せて用いることができる。 In the present invention, among the above fats and oils, it is preferable to use milk fat from the viewpoint of obtaining a salty taste enhancer having a particularly high salty taste enhancing effect. When milk fat is used, milk fat itself can be used, and milk containing milk fat such as milk, concentrated milk, condensed milk, cream, butter, butter cream, cream cheese, natural cheese, and process cheese as the above milk raw materials. And by using dairy products, milk fat can also be used, and these milks and dairy products can be used alone or in combination of two or more.

上記水中油型乳化物を得るためには水を使用することができる。使用する水については、特に限定されず、水道水、軟水系・硬水系のミネラルウォーター、イオン交換水、蒸留水等のいずれも使用可能であり、またこれらを混合して使用することもできる。
また、上記水中油型乳化物においては、水に代えて又は水に加えて、上記乳や乳製品のうちの水を多く含有する乳や乳製品、又は水を多く含有する飲食品を使用することもできるが、本発明では、牛乳、濃縮乳、ホエイ、クリーム、バター、クリームチーズ、ナチュラルチーズ、プロセスチーズ等の水分を多く含有する乳や乳製品のうちの1種又は2種以上を使用することが好ましい。
水中油型乳化物中は、水分含量が好ましくは30〜95質量%、より好ましくは70〜90質量%となるように調製される。
Water can be used to obtain the oil-in-water emulsion. The water to be used is not particularly limited, and any of tap water, soft water / hard water mineral water, ion-exchanged water, distilled water and the like can be used, and these can also be mixed and used.
Further, in the oil-in-water emulsion, instead of or in addition to water, the milk or dairy product containing a large amount of water, or the food or drink containing a large amount of water is used. However, in the present invention, one or more of milk and dairy products containing a large amount of water such as milk, concentrated milk, whey, cream, butter, cream cheese, natural cheese, and processed cheese are used. It is preferable to do so.
The oil-in-water emulsion is prepared so that the water content is preferably 30 to 95% by mass, more preferably 70 to 90% by mass.

本発明では、上記水中油型乳化物中に、遊離脂肪酸を含有させることができ、特に水中油型乳化物中に遊離脂肪酸を0.001〜0.5質量%含有させることにより、飲食品の塩味の発現パターン、特に摂取した際の最初の呈味(先味)を改善することができる。 In the present invention, the free fatty acid can be contained in the oil-in-water emulsion, and in particular, by containing 0.001 to 0.5% by mass of the free fatty acid in the oil-in-water emulsion, the food or drink can be prepared. It is possible to improve the expression pattern of salty taste, especially the initial taste (first taste) when ingested.

遊離脂肪酸は、添加する際の形態に制限はないが、上記油脂に、アルカリ等を用いた化学的分解やリパーゼ等の酵素を用いた酵素分解等により、1種又は2種以上の処理を施した、油脂分解物の形で添加されることが、より塩化ナトリウムが有する塩味の先味に近い呈味が得られる点で、特に好ましい。分解に供する油脂の種類については、特に限定されないが、乳脂の分解物を用いると、特に先味の強化を図ることのできる塩味強化剤が得られる。尚、この油脂分解物から脂肪酸のみを抽出等の操作により取り出して添加することもでき、市販されている遊離脂肪酸をそのまま添加することもできるが、遊離脂肪酸は、様々な炭素数や飽和度の脂肪酸の混合物であることが、不快臭等がなく塩味の先味を強化できるため、好ましい。 The form of free fatty acid when added is not limited, but the above fats and oils are treated with one or more types by chemical decomposition using alkali or the like or enzymatic decomposition using an enzyme such as lipase. It is particularly preferable to add it in the form of a decomposed product of fats and oils, because a taste closer to the salty taste of sodium chloride can be obtained. The type of fats and oils to be decomposed is not particularly limited, but when a decomposed product of milk fat is used, a salty taste enhancer capable of enhancing the taste can be obtained. It should be noted that only fatty acids can be extracted from this decomposition product of fats and oils by an operation such as extraction and added, or commercially available free fatty acids can be added as they are, but free fatty acids have various carbon numbers and saturations. A mixture of fatty acids is preferable because it does not have an unpleasant odor and can enhance the salty taste.

また、上記水中油型乳化物には、上記以外のその他の成分を必要に応じて添加することができる。その他の成分としては、例えば、アルギン酸、アルギン酸塩、ペクチン、LMペクチン、HMペクチン、海藻抽出物、海藻エキス、寒天、グルコマンナン、ローカストビーンガム、グアーガム、ジェランガム、タラガントガム、キサンタンガム、カラギーナン、カードラン、タマリンドシードガム、カラヤガム、タラガム、トラガントガム、アラビアガム、カシアガム、メチルセルロース、カルボキシメチルセルロース、ポリデキストロース等のゲル化剤や安定剤、金属イオン封鎖剤、食塩、岩塩等の塩味剤、無機塩、有機酸塩、無機酸、有機酸、直鎖デキストリン・分枝デキストリン・環状デキストリン・難消化性デキストリン等のデキストリン類、蔗糖、液糖、はちみつ、ブドウ糖、果糖、麦芽糖、乳糖、シクロデキストリン、酵素糖化水飴、酸糖化水飴、還元澱粉糖化物、還元糖ポリデキストロース、還元乳糖、ソルビトール、キシリトール、マルチトール、エリスリトール、マンニトール、異性化液糖、ショ糖結合水飴、オリゴ糖、キシロース、トレハロース、フラクトオリゴ糖、大豆オリゴ糖、ガラクトオリゴ糖、キシロオリゴ糖、アラビノース、パラチノースオリゴ糖、アガロオリゴ糖、キチンオリゴ糖、乳果オリゴ糖、ヘミセルロース、モラセス、イソマルトオリゴ糖、マルトオリゴ糖、ラフィノース、ラクチュロース、テアンデオリゴ糖、ゲンチオリゴ糖等の糖類、アセスルファムカリウム、スクラロース、ステビア、アスパルテーム、サッカリン、ネオテーム、甘草、羅漢果、グリチルリチン、グリチルリチン酸塩、ジヒドロカルコン、ソーマチン、モネリン等の甘味料、アルコール、プロピレングリコール、香料、苦味料・調味料等の呈味成分、着色料、保存料、酸化防止剤、pH調整剤、乳化剤、賦形剤、固結防止剤、分散剤、光沢剤、ビタミン剤等を配合することができる。 In addition, other components other than the above can be added to the oil-in-water emulsion as needed. Other ingredients include, for example, alginic acid, alginate, pectin, LM pectin, HM pectin, seaweed extract, seaweed extract, agar, glucomannan, locust bean gum, guar gum, gellan gum, taragant gum, xanthan gum, carrageenan, curdlan, Gelling agents and stabilizers such as tamarind seed gum, karaya gum, tara gum, tragant gum, arabic gum, cassia gum, methyl cellulose, carboxymethyl cellulose, polydextrin, metal ion sequestering agents, salting agents such as salt and rock salt, inorganic salts, organic acid salts. , Inorganic acid, organic acid, linear dextrin, branched dextrin, cyclic dextrin, indigestible dextrin and other dextrins, sucrose, liquid sugar, honey, glucose, fructose, malt sugar, lactose, cyclodextrin, enzyme saccharified candy, acid Saccharified water candy, reduced starch saccharified product, reduced sugar polydextrose, reduced lactose, sorbitol, xylitol, martitol, erythritol, mannitol, isomerized liquid sugar, sucrose-bound candy, oligosaccharide, xylose, trehalose, fructo-oligosaccharide, soybean oligosaccharide , Galacto-oligosaccharides, xylooligosaccharides, arabinose, palatinose oligosaccharides, agarooligosaccharides, chitin oligosaccharides, milk fruit oligosaccharides, hemicellulose, molaces, isomaltooligosaccharides, maltooligosaccharides, raffinose, lacturose, theande-oligosaccharides, gentioligosides Sweeteners such as potassium, sucrose, stevia, aspartame, saccharin, neotame, licorice, Rakanka, glycyrrhizin, glycyrrhizinate, dihydrocalcon, somatin, monerin, alcohol, propylene glycol, fragrance, bitterness / seasoning, etc. , Colorants, preservatives, antioxidants, pH adjusters, emulsifiers, excipients, anti-caking agents, dispersants, brighteners, vitamins and the like can be blended.

その他の成分の添加量は、水中油型乳化物中の固形分中で20質量%以下が好ましく、15質量%以下がより好ましく、10質量%以下が最も好ましい。
その他の成分を20質量%よりも多く入れると、飲食品に塩味強化剤を添加した際に本来有しない風味を付与する恐れがある上、飲食品が本来有しないテクスチャを付与する恐れがある。
The amount of other components added is preferably 20% by mass or less, more preferably 15% by mass or less, and most preferably 10% by mass or less in the solid content of the oil-in-water emulsion.
If more than 20% by mass of other components are added, a flavor that the food or drink does not originally have may be imparted when the salty taste enhancer is added to the food or drink, and a texture that the food or drink does not originally have may be imparted.

また、上記水中油型乳化物は、該水中油型乳化物中の油脂100質量部に対して、蛋白質が75〜250質量部、炭水化物が120〜300質量部の量比を満たすように調製することが好ましい。尚、蛋白質及び炭水化物の量は、乳原料以外のその他の成分に含まれる蛋白質及び炭水化物の量も併せて算出するものとする。 The oil-in-water emulsion is prepared so that the amount ratio of protein to 75 to 250 parts by mass and carbohydrate to 120 to 300 parts by mass is satisfied with respect to 100 parts by mass of fat and oil in the oil-in-water emulsion. Is preferable. The amount of protein and carbohydrate shall be calculated together with the amount of protein and carbohydrate contained in other components other than the dairy raw material.

上記水中油型乳化物において、油脂100質量部に対して蛋白質が75質量部未満となる場合、得られる水中油型乳化物の乳化安定性が低下する恐れがあることに加え、得られる本発明の塩味強化剤及び飲食品の風味の低下や、塩化ナトリウム含量の低減効果が低下する恐れがある。また蛋白質が250質量部超となる場合、乳酸発酵中の蛋白質分解の際に呈味成分や香気成分が過剰に産生され、得られる本発明の塩味強化剤及び飲食品に異味を付与してしまう恐れがある。
また、上記水中油型乳化物において、油脂100質量部に対して炭水化物が120質量部未満の場合、乳酸発酵の基質が不足し、風味成分や香気成分の産生が十分になされない恐れがある上、ナトリウム低減効果が低下する恐れがある。また炭水化物が250質量部超となる場合、得られる本発明の塩味強化剤及び飲食品に異味・雑味を付与してしまう恐れがある。
In the above oil-in-water emulsion, if the amount of protein is less than 75 parts by mass with respect to 100 parts by mass of fat and oil, the emulsion stability of the obtained oil-in-water emulsion may be lowered, and the obtained invention of the present invention. There is a risk that the salty taste enhancer and the flavor of foods and drinks will be reduced, and the effect of reducing the sodium chloride content will be reduced. Further, when the amount of protein exceeds 250 parts by mass, a taste component and an aroma component are excessively produced during protein decomposition during lactic acid fermentation, and the obtained salty taste enhancer and food and drink of the present invention are given an unpleasant taste. There is a fear.
Further, in the above-mentioned oil-in-water emulsion, if the amount of carbohydrate is less than 120 parts by mass with respect to 100 parts by mass of fats and oils, the substrate for lactic acid fermentation may be insufficient, and the production of flavor components and aroma components may not be sufficient. , Sodium reduction effect may be reduced. Further, when the amount of carbohydrate exceeds 250 parts by mass, the obtained salty taste enhancer and food / drink of the present invention may be imparted with an unpleasant taste or an unpleasant taste.

次に、上記乳原料を含む水中油型乳化物の製造方法について述べる。
上記乳原料を含む水中油型乳化物の製造方法は特に限定されない。例えば、牛乳、濃縮乳、ホエイ、クリーム、クリームチーズ、ナチュラルチーズ、プロセスチーズ等の水分を多く含有する乳や乳製品、あるいは水に、脱脂粉乳、全粉乳、ホエイパウダー等の粉乳類や、乳蛋白質、乳糖等の乳原料を添加して水相とした後、該水相に、油脂等を含む油相、又は油脂を多く含有する食品素材、特に、バター、クリーム、クリームチーズ、ナチュラルチーズ、プロセスチーズ等の乳脂を含有する乳原料を添加し、これを水中油型に乳化する方法を挙げることができる。尚、その他の原料については、水相に添加することも油相に添加することもでき、また別途添加することもできる。
Next, a method for producing an oil-in-water emulsion containing the above dairy raw material will be described.
The method for producing an oil-in-water emulsion containing the above dairy raw material is not particularly limited. For example, milk or dairy products containing a large amount of water such as milk, concentrated milk, whey, cream, cream cheese, natural cheese, process cheese, or milk powder such as nonfat dry milk, whole milk powder, whey powder, or milk. After adding dairy ingredients such as protein and milk sugar to make an aqueous phase, the aqueous phase contains an oil phase containing fats and oils, or a food material containing a large amount of fats and oils, particularly butter, cream, cream cheese, and natural cheese. A method of adding a milk raw material containing milk fat such as processed cheese and emulsifying it into an oil-in-water mold can be mentioned. The other raw materials can be added to the aqueous phase, the oil phase, or separately.

この後、得られた水中油型乳化物を安定なものとするために、均質化を行うことが好ましい。均質化を行なうための均質化機としては、例えば、ケトル型チーズ乳化釜、ステファンミキサー等の高速せん断乳化釜、スタティックミキサー、インラインミキサー、ホモゲナイザー、コロイドミル、ディスパーミル等が挙げられ、好ましくは1〜200MPaの均質化圧力にて均質化を行なう。 After this, it is preferable to perform homogenization in order to stabilize the obtained oil-in-water emulsion. Examples of the homogenizing machine for performing homogenization include a kettle-type cheese emulsifying kettle, a high-speed shear emulsifying kettle such as a Stefan mixer, a static mixer, an in-line mixer, a homogenizer, a colloid mill, and a disper mill, and 1 is preferable. Homogeneity is performed at a homogenization pressure of ~ 200 MPa.

均質化後、更に必要に応じて、加熱殺菌を行なう。該加熱殺菌の方法としては、インジェクション式、インフュージョン式、マイクロ波、ジュール加熱式等の直接加熱方式、又は、バッチ式、プレート式、チューブラー式、掻き取り式等の間接加熱方式があり、UHT、HTST、LTLT等の50〜160℃、好ましくは55〜140℃の加熱処理を行なうことができる。また、加熱殺菌後に必要に応じて、再度均質化の他、急速冷却、徐冷却等の冷却操作を施すことができる。 After homogenization, heat sterilization is further performed if necessary. The heat sterilization method includes a direct heating method such as an injection type, an infusion type, a microwave, and a Joule heating type, or an indirect heating method such as a batch type, a plate type, a tubular type, and a scraping type. UHT, HTST, LTLT and the like can be heat-treated at 50 to 160 ° C, preferably 55 to 140 ° C. Further, after heat sterilization, if necessary, in addition to homogenization, cooling operations such as rapid cooling and slow cooling can be performed.

本発明では、上記水中油型乳化物を乳酸発酵の基質であるミックス液として使用する。乳酸発酵に使用する乳酸菌としては、特に限定されるものではないが、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis、Lactobacillus casei subsp. casei、Lactobacillus acidophilus、Lactobacillus delbrueckii subsp. delbrueckii、Lactobacillus delbrueckii subsp. bulgaricus、Lactobacillus delbrueckii subsp. lactis、Lactobacillus jugurti、Lactobacillus helveticus、Lactobacillus kefyr、Lactobacillus plantarum、Lactobacillus rhamnosus、Streptococcus thermophilus、Leuconostoc mesenteroides subsp. cremoris、Bifidobacterium longum、Bifidobacterium bifidum、Bifidobacterium infantis、Bifidobacterium breve等が挙げられる。これらは単独で用いることができ、又は二種以上を組み合わせて用いることもできる。 In the present invention, the oil-in-water emulsion is used as a mixed solution which is a substrate for lactic acid fermentation. The lactic acid bacteria used for lactic acid fermentation are not particularly limited, but Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lacttis biovar diacetylactis, Lactobacillus casei subsp. Casei, Lactobacillus acidlus . delbrueckii, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactobacillus jugurti, Lactobacillus helveticus, Lactobacillus kefyr, Lactobacillus plantarum, Lactobacillus rhamnosus, Streptococcus thermophilus, Leuconostoc mesenteroides subsp. cremoris, Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium breve And so on. These can be used alone or in combination of two or more.

本発明では、良好な風味・香気を有し、ナトリウム低減効果が高い乳酸発酵物が得られる点で、Lactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis、Streptococcus thermophilus、及びLeuconostoc mesenteroides subsp. cremorisのうちの1種又は2種以上を用いることが好ましい。 In the present invention, Lactococcus helveticus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lactis biovar diacetylactis, can be obtained in that a lactic acid fermented product having a good flavor and aroma and a high sodium reducing effect can be obtained. It is preferable to use one or more of Streptococcus thermophilus and Leuconostoc mesenteroides subsp. Cremoris.

本発明では、脂質資化性及び/又は遊離脂肪酸資化性の高い乳酸菌を使用することが、得られる乳酸発酵物の香気成分が一層複雑になることから、飲食品におけるナトリウム低減効果が高くなる点で好ましい。
脂質資化性又は遊離脂肪酸資化性の高い乳酸菌の一例としては、Streptococcus thermophilus、Leuconostoc mesenteroides subsp. cremoris等が挙げられる。
In the present invention, the use of lactic acid bacteria having high lipid assimilation and / or free fatty acid assimilation makes the aroma component of the obtained lactic acid fermented product more complicated, so that the sodium reducing effect in foods and drinks is enhanced. It is preferable in that respect.
Examples of lactic acid bacteria having high lipid assimilation or free fatty acid assimilation include Streptococcus thermophilus and Leuconostoc mesenteroides subsp. Cremoris.

即ち、本発明では、Lactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis、Streptococcus thermophilus、及びLeuconostoc mesenteroides subsp. cremorisのうち、Streptococcus thermophilus及び/又はLeuconostoc mesenteroides subsp. cremorisを含む1種又は2種以上を用いることが好ましく、より好ましくはLactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis、Streptococcus thermophilus、及びLeuconostoc mesenteroides subsp. cremorisの6種を併用する。 That is, in the present invention, among Lactobacillus helveticus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lacttis biovar diacetylactis, Streptococcus thermophilus, and Leuconostoc mesenteroides subsp. It is preferable to use one or more species containing cremoris, more preferably Lactococcus helveticus, Lactococcus lactis subsp. Lacttis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lacttis biovar diacetylactis, Streptococcus thermophilus, and Leucon 6 types are used together.

乳酸菌の添加量は、後述するpHの乳酸発酵物を効率よく得る観点から、上記水中油型乳化物100質量部に対して、乾燥質量で0.0001質量部以上0.2質量部以下であることが好ましく、0.004質量部以上0.05質量部以下であることがより好ましい。 The amount of lactic acid bacteria added is 0.0001 parts by mass or more and 0.2 parts by mass or less in terms of dry mass with respect to 100 parts by mass of the oil-in-water emulsion from the viewpoint of efficiently obtaining a lactic acid fermented product having a pH described later. It is preferable, and it is more preferable that it is 0.004 part by mass or more and 0.05 part by mass or less.

乳酸発酵における温度条件については、使用する乳酸菌の至適温度域を考慮した上で適宜設定すればよいが、発酵乳酸菌の死滅を防ぐために、乳酸発酵中の発酵液の液温は60℃未満であることが好ましい。 The temperature conditions for lactic acid fermentation may be set appropriately in consideration of the optimum temperature range of the lactic acid bacteria to be used, but in order to prevent the death of the fermenting lactic acid bacteria, the liquid temperature of the fermented liquid during the lactic acid fermentation is less than 60 ° C. It is preferable to have.

乳酸発酵時間は基質濃度や乳酸菌の添加量等に応じ適宜選択可能であるが、好ましくは、乳酸発酵物の発酵終点はpHによって適宜判断される。具体的には、pH2.8〜5.5の間で発酵を停止するのが好ましく、pH3.0〜5.0の間で発酵を停止するのが更に好ましく、pH3.5〜5.0の間で発酵を停止するのが最も好ましい。pH2.8よりも低いpHを発酵終点とした場合、乳酸発酵物の酸味が強すぎ、飲食品に酸味が付与されてしまう恐れがあり、pH5.5よりも高いpHを発酵終点とした場合、本発明の塩味強化剤の塩味強化効果が乏しくなる恐れがある。 The lactic acid fermentation time can be appropriately selected according to the substrate concentration, the amount of lactic acid bacteria added, and the like, but preferably, the fermentation end point of the lactic acid fermentation product is appropriately determined by the pH. Specifically, it is preferable to stop the fermentation between pH 2.8 and 5.5, and it is more preferable to stop the fermentation between pH 3.0 and 5.0, and it is more preferable to stop the fermentation between pH 3.5 and 5.0. Most preferably, the fermentation is stopped between. When the pH lower than pH 2.8 is set as the fermentation end point, the acidity of the lactic acid fermented product is too strong and the food and drink may be given sourness. When the pH higher than pH 5.5 is set as the fermentation end point, The salty taste enhancer of the present invention may have a poor salty taste enhancing effect.

乳酸発酵時において、乳酸菌を添加したミックス液は静置状態とすることができるが、好ましくは攪拌を行なう。好ましい攪拌条件は、1分間に5〜50回転、より好ましくは10〜30回転である。 At the time of lactic acid fermentation, the mixed solution to which lactic acid bacteria are added can be in a stationary state, but is preferably stirred. Preferred stirring conditions are 5 to 50 revolutions per minute, more preferably 10 to 30 revolutions.

発酵終点pH、即ち、乳酸発酵によってpHが低下し、乳酸菌の生育が抑制された状態を迎えた後、発酵を停止させる。あるいは、発酵終点pHが至適pHを下回る場合、至適pH域に達した時点で発酵を停止させる。発酵停止の手段としては加熱殺菌が通常実施される。この時の加熱温度及び加熱時間については十分に乳酸菌が失活する条件が適宜選択され、例えば65℃で30分程度行うことが望ましい。 Fermentation end point pH, that is, after the pH is lowered by lactic acid fermentation and the growth of lactic acid bacteria is suppressed, fermentation is stopped. Alternatively, when the fermentation end point pH is lower than the optimum pH, the fermentation is stopped when the optimum pH range is reached. Heat sterilization is usually carried out as a means for stopping fermentation. Regarding the heating temperature and heating time at this time, conditions for sufficiently inactivating lactic acid bacteria are appropriately selected, and it is desirable to carry out the heating at 65 ° C. for about 30 minutes, for example.

得られた乳酸発酵物中には、乳酸をはじめとする有機酸の他、炭化水素、アルコール類、アルデヒド類、エステル類、含硫化合物、ケトン類、脂肪酸類、脂肪酸エステル類、芳香族化合物、ラクトン類等の風味成分が、上記の水中油型乳化物を基質とする乳酸発酵により産生され、含有されている。
尚、得られた乳酸発酵物は、濾紙やフィルタによる濾過、精密ろ過、限外濾過、遠心分離等を行うことができる。
In the obtained lactic acid fermented product, in addition to organic acids such as lactic acid, hydrocarbons, alcohols, aldehydes, esters, sulfur-containing compounds, ketones, fatty acids, fatty acid esters, aromatic compounds, etc. Flavor components such as lactones are produced and contained by lactic acid fermentation using the above oil-in-water emulsion as a substrate.
The obtained lactic acid fermented product can be filtered with a filter paper or a filter, microfiltration, ultrafiltration, centrifugation or the like.

本発明の塩味強化剤は、上記乳酸発酵物を有効成分として含んでなり、塩化ナトリウム由来の塩味であっても塩化カリウム等の塩化ナトリウム代替物由来の塩味であっても隔てなく、塩味剤(飲食品中に塩味付与を目的として使用される塩味物質)が有する塩味を増強させることが可能である。 The salty taste enhancer of the present invention contains the above-mentioned lactic acid fermented product as an active ingredient, and does not distinguish between a salty taste derived from sodium chloride and a salty taste derived from a sodium chloride substitute such as potassium chloride. It is possible to enhance the salty taste of (a salty substance used for the purpose of imparting salty taste) in foods and drinks.

飲食品において、本発明の塩味強化剤は、例えば、塩味剤1質量部に対して、本発明の塩味強化剤中の上記乳酸発酵物の固形分が0.00001〜0.1質量部となるように添加されるのが好ましく、0.0005〜0.004質量部となるように添加されるのがより好ましく、0.001〜0.003質量部となるように添加されるのが更に好ましい。 In foods and drinks, the salty taste enhancer of the present invention has, for example, 0.00001 to 0.1 parts by mass of the solid content of the lactic acid fermented product in the salty taste enhancer of the present invention with respect to 1 part by mass of the salty agent. It is preferable that it is added so as to be 0.0005 to 0.004 parts by mass, and it is further preferable that it is added so as to be 0.001 to 0.003 parts by mass. ..

本発明の塩味強化剤は、上記乳酸発酵物に加え、食塩様の塩味強度や塩味発現パターンを得る上で、乳清ミネラル及び/又はエキス類を含有することが好ましい。 The salty taste enhancer of the present invention preferably contains whey minerals and / or extracts in order to obtain a salty salty taste intensity and a salty taste expression pattern in addition to the above lactic acid fermented product.

<乳清ミネラル>
乳清ミネラルとは、乳清より乳清タンパクと乳糖を分離除去し、精製して得られたものである。本発明の塩味強化剤中に乳清ミネラルを含有させると、飲食品中の塩化ナトリウム含量を低減させる前後で同等の塩味強度や塩味発現パターンが得られやすくなる上に、特に塩味剤中に塩化カリウムを含む場合に特有の苦みを和らげる効果が得られるため好ましい。
<Whey minerals>
Whey minerals are obtained by separating and removing whey protein and lactose from whey and purifying them. When the salty taste enhancer of the present invention contains milky minerals, it becomes easy to obtain the same salty taste intensity and salty taste development pattern before and after reducing the sodium chloride content in foods and drinks, and in particular, chloride in the salty taste agent. It is preferable because it has the effect of relieving the bitterness peculiar to the case where potassium is contained.

本発明で使用する乳清ミネラルは、塩味強化剤に対する異味や雑味の付与を防ぐ目的から、蛋白質や乳糖等の不純物含量が低いことが好ましく、固形分に占める灰分含量が30質量%以上である乳清ミネラルを使用することが好ましく、固形分に占める灰分含量が50質量%以上である乳清ミネラルを使用することがより好ましい。尚、該灰分含量は高いほど好ましい。 The whey mineral used in the present invention preferably has a low impurity content such as protein and lactose for the purpose of preventing the addition of unpleasant taste and unpleasant taste to the salty taste enhancer, and the ash content in the solid content is 30% by mass or more. It is preferable to use a certain whey mineral, and it is more preferable to use a whey mineral having an ash content of 50% by mass or more in the solid content. The higher the ash content, the more preferable.

加えて、使用する乳清ミネラルは、その固形分中のカルシウム含量が好ましくは2質量%未満、より好ましくは1質量%未満、更に好ましくは0.5質量%未満の乳清ミネラルであることが好ましい。 In addition, the whey mineral used may be a whey mineral having a calcium content in its solid content of preferably less than 2% by mass, more preferably less than 1% by mass, still more preferably less than 0.5% by mass. preferable.

本発明の塩味強化剤において、乳清ミネラルは、乳酸発酵物の固形分1質量部に対して、乳清ミネラル中の固形分が0.001〜1.0質量部となるように含有させることが好ましく、より好ましくは0.005〜0.3質量部、最も好ましくは0.05〜0.3質量部となるように含有させる。
0.001質量部未満の場合、乳清ミネラルの添加効果が得られない。また、1.0質量部超の場合、乳清ミネラルが有する雑味が飲食品に付与されてしまう恐れがある。
In the salty taste enhancer of the present invention, the whey mineral is contained so that the solid content in the whey mineral is 0.001 to 1.0 part by mass with respect to 1 part by mass of the solid content of the lactic acid fermented product. Is more preferable, and the content is more preferably 0.005 to 0.3 parts by mass, and most preferably 0.05 to 0.3 parts by mass.
If it is less than 0.001 part by mass, the effect of adding whey mineral cannot be obtained. On the other hand, if it exceeds 1.0 part by mass, the miscellaneous taste of whey minerals may be added to foods and drinks.

本発明で使用する際の乳清ミネラルの形態は特に限定されず、液体の状態であっても、粉末状・顆粒状・錠剤等の固体の状態であっても使用可能であるが、粉末状又は液体の状態であることが、乳酸発酵物やエキス類等と均一に混合しやすいため好ましい。 The form of the whey mineral when used in the present invention is not particularly limited, and it can be used in a liquid state or in a solid state such as powder, granules, tablets, etc., but in powder form. Alternatively, it is preferable that it is in a liquid state because it can be easily mixed uniformly with lactic acid fermented products, extracts and the like.

<エキス類>
本発明におけるエキス類とは、畜産物、水産物、農産物及び微生物の1種又は2種以上から得られた抽出物を指す。具体的には、チキンエキス・ビーフエキス・ポークエキス等の畜産エキス、鰹エキス・鯖エキス・鮭エキス・鰹節エキス・煮干エキス・グチエキス・ハモエキス・タラエキス・イカエキス・カキエキス・アワビエキス・ホタテエキス・エビエキス・カニエキス・オキアミエキス・オイスターエキス・アサリエキス・昆布エキス・ワカメエキス・、魚醤エキス等の水産エキス、モルトエキス・玉葱エキス・人参エキス・白菜エキス・キャベツエキス・セロリエキス・にんにくエキス・ジンジャーエキス・椎茸エキス・マッシュルームエキス・果実エキス等の農産エキス、ビール酵母エキス・パン酵母エキス・トルラ酵母エキス等の酵母エキス等を挙げることができる。本発明ではこれらのエキス類の中から選ばれた1種又は2種以上を用いることができるが、好ましくは酵母エキスを使用する。
<Extracts>
The extracts in the present invention refer to extracts obtained from one or more of livestock products, marine products, agricultural products and microorganisms. Specifically, livestock extracts such as chicken extract, beef extract, pork extract, eel extract, mackerel extract, salmon extract, sardine extract, boiled dried extract, glutinous extract, hamo extract, cod extract, squid extract, oyster extract, abalone extract, scallop extract, shrimp extract, etc. Crab extract, okiami extract, oyster extract, asari extract, kelp extract, wakame extract, marine extract such as fish soy sauce extract, malt extract, onion extract, carrot extract, white vegetable extract, cabbage extract, celery extract, garlic extract, ginger extract, etc. Agricultural extracts such as shiitake mushroom extract, mushroom extract and fruit extract, yeast extracts such as beer yeast extract, bread yeast extract and torula yeast extract can be mentioned. In the present invention, one or more selected from these extracts can be used, but yeast extract is preferably used.

本発明の塩味強化剤に上記エキス類を含有させると、飲食品中の塩化ナトリウム含量を低減させる前後で同等の塩味強度や塩味発現パターンが得られやすくなる上に、特に塩味剤中に塩化カリウムを含む場合に塩カドを和らげる効果が得られるため好ましい。 When the salty taste enhancer of the present invention contains the above extracts, it becomes easy to obtain the same salty taste intensity and salty taste expression pattern before and after reducing the sodium chloride content in foods and drinks, and in particular, potassium chloride in the salty taste agent. It is preferable because it has the effect of softening salty acid when it contains.

上記エキス類は、塩味強化剤中に均一に含有させることができるものであれば、液状やペースト状、又は固体状(粉体、顆粒状、錠剤)等、どのような形態のものでも使用することができる。尚、上記のエキス類が液状やペースト状である場合、その固形分含量は好ましくは20〜80質量%、より好ましくは40〜70質量%である。 The above extracts may be used in any form such as liquid, paste, or solid (powder, granule, tablet) as long as they can be uniformly contained in the salty taste enhancer. be able to. When the above extracts are in the form of a liquid or a paste, the solid content thereof is preferably 20 to 80% by mass, more preferably 40 to 70% by mass.

本発明の塩味強化剤において、上記エキス類は、乳酸発酵物の固形分1質量部に対して、エキス類が0.001〜10質量部となるように含有させることが好ましく、より好ましくは0.02〜3.0質量部、最も好ましくは0.1〜3.0質量部となるように含有させる。
0.001質量部未満の場合、エキス類の添加効果が得られない。また、10質量部超の場合、エキス類が有する旨味が付与されてしまい、塩味強化剤が添加された飲食品の風味を変えてしまう恐れがある。
In the salty taste enhancer of the present invention, the above extracts are preferably contained so that the amount of the extracts is 0.001 to 10 parts by mass with respect to 1 part by mass of the solid content of the lactic acid fermented product, and more preferably 0. It is contained in an amount of .02 to 3.0 parts by mass, most preferably 0.1 to 3.0 parts by mass.
If it is less than 0.001 part by mass, the effect of adding the extracts cannot be obtained. On the other hand, if it exceeds 10 parts by mass, the umami of the extracts is imparted, which may change the flavor of foods and drinks to which the salty taste enhancer is added.

<塩味強化剤の形態>
本発明の塩味強化剤の形態は、固体とすることも液体とすることもでき、本発明の塩味強化剤が使用される飲食品の形態によって任意に選択される。
本発明の塩味強化剤を製造する際の各成分の混合方法や混合順序については、得られる塩味強化剤に含有される成分が均一に混合されれば、特に制限はない。塩味強化剤を製造する際に用いる原料の形態としては、粉体等の固体原料、及び液体原料等を任意に選択することができる。本発明の塩味強化剤は、一度水溶液とした後に、溶媒を留去して、均一な粉体に調製することも可能であり、溶媒を留去する場合には任意の方法で溶媒留去を行うことができ、例えば噴霧乾燥や造粒乾燥等の方法で溶媒留去を行うことができる。
<Form of salty taste enhancer>
The form of the salty taste enhancer of the present invention may be solid or liquid, and is arbitrarily selected depending on the form of the food or drink in which the salty taste enhancer of the present invention is used.
The mixing method and mixing order of each component in producing the salty taste enhancer of the present invention are not particularly limited as long as the components contained in the obtained salty taste enhancer are uniformly mixed. As the form of the raw material used in producing the salty taste enhancer, a solid raw material such as powder, a liquid raw material or the like can be arbitrarily selected. The salty taste enhancer of the present invention can be prepared into a uniform powder by distilling off the solvent after making an aqueous solution once. When distilling off the solvent, distilling off the solvent by any method is possible. The solvent can be distilled off by a method such as spray drying or granulation drying.

<飲食品>
本発明の飲食品は、本発明の塩味強化剤を用いた飲食品である。
ここで、上記飲食品とは、一般に塩化ナトリウムを主成分とする食塩を使用する食品全般を指し、特に限定されるものではない。例えば、味噌、醤油、麺つゆ、たれ、出汁、パスタソース、ドレッシング、マヨネーズ、トマトケチャップ、ウスターソース、とんかつソース、ふりかけ、調味塩等の調味料、お吸い物の素、カレールウ、ホワイトソース、お茶漬けの素、スープの素等の即席調理食品、味噌汁、お吸い物、コンソメスープ、ポタージュスープ等のスープ類、ビーフジャーキー、ハム、ソーセージ、チーズ等の畜産加工品、かまぼこ、干物、塩辛、佃煮、珍味等の水産加工品、漬物等の野菜加工品、ポテトチップス、煎餅等の菓子スナック類、食パン、菓子パン、クッキー等のベーカリー食品類、煮物、揚げ物、焼き物、カレー、シチュー、グラタン、ごはん、おかゆ、おにぎり等の調理食品等が挙げられる。尚、食塩を含有しない飲食品であっても、飲食時に食塩が含まれる飲食品であれば、本発明の塩味強化剤を使用することができる。
<Food and drink>
The food and drink of the present invention is a food and drink using the salty taste enhancer of the present invention.
Here, the above-mentioned food and drink generally refers to all foods using salt containing sodium chloride as a main component, and is not particularly limited. For example, miso, soy sauce, noodle soup, sauce, soup, pasta sauce, dressing, mayonnaise, tomato ketchup, Worcestershire sauce, tonkatsu sauce, sprinkle, seasoning salt, etc. , Instant cooked foods such as soup base, miso soup, soup, consomme soup, potage soup and other soups, processed livestock products such as beef jerky, ham, sausage, cheese, kamaboko, dried food, salted food, boiled rice, delicacies, etc. Processed marine products, processed vegetables such as pickles, sweets snacks such as potato chips and roasted rice cakes, bakery foods such as bread, sweet bread and cookies, simmered foods, fried foods, grilled foods, curry, stew, gratin, rice, rice porridge, rice balls, etc. Cooked foods and the like. Even if the food or drink does not contain salt, the salty taste enhancer of the present invention can be used as long as the food or drink contains salt when eating or drinking.

<減塩飲食品>
本発明の飲食品は、その味質を維持しながら塩化ナトリウム含量当たりの塩味が強化されている点が特徴として挙げられる。その塩化ナトリウムの一部を塩化カリウム等の塩化ナトリウム代替物で置換した飲食品である場合は、塩化ナトリウム含量あたりの塩味が更に強化されたものとすることができる。つまり、本発明の塩味強化剤を添加し、従来の飲食品に含まれる食塩の量を低減する場合、食塩使用量を低減させたとしても従来の飲食品と同等の塩味と同等の味質を有する飲食品とすることができ、減塩飲食品として好ましく使用することができる。そのため、従来の塩化ナトリウム含量を減じただけの減塩飲食品や、塩化ナトリウムの一部又は全部を塩化ナトリウム代替物で置換した減塩飲食品に比べ、味質が良好である減塩飲食品とすることができる。
<Reduced salt food and drink>
The food and drink of the present invention is characterized in that the salty taste per sodium chloride content is enhanced while maintaining its taste quality. In the case of a food or drink in which a part of the sodium chloride is replaced with a sodium chloride substitute such as potassium chloride, the salty taste per sodium chloride content can be further enhanced. That is, when the salty taste enhancer of the present invention is added to reduce the amount of salt contained in conventional foods and drinks, even if the amount of salt used is reduced, the salty taste equivalent to that of conventional foods and drinks is obtained. It can be a food or drink to have, and can be preferably used as a low-salt food or drink. Therefore, compared to conventional low-salt foods and drinks in which the sodium chloride content is simply reduced, and low-salt foods and drinks in which part or all of sodium chloride is replaced with a sodium chloride substitute, low-salt foods and drinks have better taste. Can be.

本発明において、減塩飲食品とは、通常の飲食品よりも塩化ナトリウム含量が10〜90質量%、好ましくは20〜80質量%、更に好ましくは30〜70質量%減じられた食品をいう。塩化ナトリウム含量を減じた割合が10質量%未満であると、減塩飲食品としての優位性に乏しく、90質量%を超えて減ずると、本発明の塩味強化剤によっても、同等の強さの塩味を得ることが難しくなってしまう。 In the present invention, the low-salt food or drink refers to a food in which the sodium chloride content is reduced by 10 to 90% by mass, preferably 20 to 80% by mass, and more preferably 30 to 70% by mass as compared with ordinary foods and drinks. If the ratio of reduced sodium chloride content is less than 10% by mass, the superiority of the salt-reduced food and drink is poor, and if it is reduced by more than 90% by mass, the saltiness enhancer of the present invention has the same strength. It becomes difficult to obtain salty taste.

上記飲食品に本発明の塩味強化剤を含有させる方法としては、飲食品の製造時又は飲食時に本発明の塩味強化剤を添加する方法を主に用いることができ、喫食時までに、好ましくは前述した比率で、塩味剤と本発明の塩味強化剤が飲食品中に含まれていればよい。
例えば、塩味剤を含有する飲食品に本発明の塩味強化剤を添加する方法、塩味剤を含有する飲食品の製造時に本発明の塩味強化剤を添加する方法、塩味剤に本発明の塩味強化剤を添加した調味塩を飲食品の製造時に使用する方法、該調味塩を飲食品に添加する方法、塩味剤を含有する飲食品に、塩味強化剤を含有する飲食品を添加する方法等を挙げることができる。
As a method for incorporating the salty taste enhancer of the present invention into the above-mentioned food or drink, a method of adding the salty taste enhancer of the present invention at the time of manufacturing the food or drink or at the time of eating or drinking can be mainly used, and is preferably used by the time of eating. It suffices that the salty agent and the salty taste enhancer of the present invention are contained in the food or drink in the above-mentioned ratio.
For example, a method of adding the salty taste enhancer of the present invention to a food or drink containing a salty agent, a method of adding a salty taste enhancer of the present invention at the time of producing a food or drink containing a salty agent, or a method of adding the salty taste enhancer of the present invention to a salty agent. A method of using the seasoned salt to which the agent is added in the production of food and drink, a method of adding the seasoned salt to the food and drink, a method of adding the food and drink containing a salty taste enhancer to the food and drink containing the salty agent, and the like. Can be mentioned.

また、本発明の塩味強化剤は塩化カリウム等の塩化ナトリウム代替品の風味の改善にも効果があることから、塩味が物足りない減塩飲食品に対し、塩化カリウム等の塩化ナトリウム代替品と共に本発明の塩味強化剤を添加する方法を採ることも可能である。 Further, since the salty taste enhancer of the present invention is also effective in improving the flavor of a sodium chloride substitute such as potassium chloride, the present invention is used together with a sodium chloride substitute such as potassium chloride for a low-salt food or drink having an unsatisfactory salty taste. It is also possible to adopt the method of adding the salty taste enhancer.

以下、本発明を具体的な実施例により詳述するが、本発明は下記実施例の記載により何ら制限されない。 Hereinafter, the present invention will be described in detail with reference to specific examples, but the present invention is not limited by the description of the following examples.

<乳酸発酵物の製造>
〔製造例1〕
水83.0質量%に対して、脱脂粉乳(無脂乳固形分95.2質量%、蛋白質含量34質量%、脂質1質量%)4.5質量%、ホエイパウダー(無脂乳固形分95質量%、蛋白質含量13質量%、脂質1質量%)3.5質量%、トータルミルクプロテイン(無脂乳固形分91質量%、蛋白質含量81質量%、脂質4質量%)1質量%、及び脱脂濃縮乳(無脂乳固形分25.6質量%、蛋白質含量9.2質量%、脂質3.5質量%)4質量%を加え、水浴の温度を60℃に維持しながら撹拌し、十分に分散・溶解を行った後、無塩バター(無脂乳固形分1.20質量%、脂質83質量%)2質量%、及びクリームチーズ(無脂乳固形分14質量%、脂質55質量%)2質量%を加え、水浴の温度を60℃に維持したまま更に撹拌し、水中油型に乳化を行った。
原料のダマがみられなくなるまで撹拌を行った後、三和エンジニアリング社製圧力式ホモゲナイザーH20型にて均質圧力20MPaで均質化し、プレート式熱交換器にて80℃で3分間加熱殺菌後、プレート式熱交換器にて30℃に冷却し、無脂乳固形分含量が9.8質量%であり油脂含量が2.9質量%である、乳原料を含む水中油型乳化物を調製した。
<Manufacturing of lactic acid fermented products>
[Manufacturing Example 1]
Skimmed milk powder (non-fat milk solid content 95.2% by mass, protein content 34% by mass, lipid 1% by mass) 4.5% by mass, whey powder (non-fat milk solid content 95% by mass) with respect to 83.0% by mass of water Mass%, protein content 13% by mass, lipid 1% by mass) 3.5% by mass, total milk protein (non-fat milk solids content 91% by mass, protein content 81% by mass, lipid 4% by mass) 1% by mass, and skim milk 4% by mass of concentrated milk (non-fat milk solid content 25.6% by mass, protein content 9.2% by mass, lipid 3.5% by mass) was added, and the mixture was stirred while maintaining the temperature of the water bath at 60 ° C., and sufficiently. After dispersion and dissolution, 2% by mass of unsalted butter (non-fat milk solids 1.20% by mass, 83% by mass of lipids) and cream cheese (14% by mass of non-fat milk solids, 55% by mass of lipids). 2% by mass was added, and the mixture was further stirred while maintaining the temperature of the water bath at 60 ° C., and emulsified into an oil-in-water mold.
After stirring until no lumps of raw materials are observed, homogenize with a pressure homogenizer H20 manufactured by Sanwa Engineering Co., Ltd. at a homogeneous pressure of 20 MPa, heat sterilize at 80 ° C. for 3 minutes with a plate heat exchanger, and then plate. The mixture was cooled to 30 ° C. in a heat exchanger to prepare an oil-in-water emulsion containing a dairy raw material having a non-fat milk solid content content of 9.8% by mass and a fat content of 2.9% by mass.

続いて、この乳原料を含む水中油型乳化物をそのままミックス液として使用し、ミックス液100質量部に対し、Lactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis 、Streptococcus thermophilus、Leuconostoc mesenteroides subsp. cremoris の6種を含む、フリーズドライタイプの乳酸菌スターターを0.04質量部加えて、30℃で15回転/分で撹拌しながら12時間発酵した。尚、乳酸菌スターターを加えた時点でのpHは6.4であり、乳酸発酵工程終了時点でのpHは4.5であった。上記乳酸発酵工程を経て、固形分が12.3質量%である乳酸発酵物Aを得た。 Subsequently, the oil-in-water emulsion containing this dairy raw material was used as it was as a mixed solution, and Lactobacillus helveticus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. 0.04 parts by mass of a freeze-dry type lactic acid bacterium starter containing 6 types of diacetylactis, Streptococcus thermophilus, and Leuconostoc mesenteroides subsp. Cremoris was added, and fermentation was carried out at 30 ° C. for 12 hours with stirring at 15 rpm. The pH at the time when the lactic acid bacterium starter was added was 6.4, and the pH at the end of the lactic acid fermentation step was 4.5. Through the above lactic acid fermentation step, a lactic acid fermented product A having a solid content of 12.3% by mass was obtained.

〔製造例2〕
製造例1の配合のうち、無塩バターを2質量%から1.7質量%に減じ、減じた分の0.3質量%を遊離脂肪酸源としての大洋香料社製デリシャンバターテイストHB−2に置き換えたこと以外は、製造例1と同様にして、無脂乳固形分含量が9.8質量%であり油脂含量が2.9質量%である、乳原料を含む水中油型乳化物を調製した。続いて、この水中油型乳化物を製造例1と同様の方法で発酵し、固形分が12.3質量%である乳酸発酵物Bを得た。
[Manufacturing Example 2]
Of the formulation of Production Example 1, unsalted butter was reduced from 2% by mass to 1.7% by mass, and 0.3% by mass of the reduced amount was used as a free fatty acid source. An oil-in-water emulsion containing a dairy raw material having a non-fat milk solid content content of 9.8% by mass and a fat content of 2.9% by mass was prepared in the same manner as in Production Example 1 except that it was replaced with. Prepared. Subsequently, this oil-in-water emulsion was fermented in the same manner as in Production Example 1 to obtain a lactic acid fermented product B having a solid content of 12.3% by mass.

〔製造例3〕
水28.2質量%に対して、豆乳(マルサンアイ株式会社製、無脂固形分6.3質量%、蛋白質含量4.7質量%、脂質含量3.1質量%)65.3質量%、及びフジプロF(不二製油社製大豆蛋白質、無脂固形分94.3質量%、蛋白質含量85.8質量%、脂質含量0.20質量%)5.5質量%を加えて、水浴の温度を60℃に維持しながら撹拌し、十分に分散・溶解を行った後、大豆油1質量%を加えて更に撹拌し、水中油型に乳化を行った。
原料のダマがみられなくなるまで撹拌を行った後、三和エンジニアリング社製圧力式ホモゲナイザーH20型にて均質圧力20MPaで均質化し、プレート式熱交換器にて80℃で3分間加熱殺菌後、プレート式熱交換器にて30℃に冷却し、無脂固形分含量が9.3質量%であり油脂含量が3.0質量%である、水中油型乳化物を調製した。
[Manufacturing Example 3]
Soymilk (manufactured by Marusan Eye Co., Ltd., fat-free solid content 6.3% by mass, protein content 4.7% by mass, lipid content 3.1% by mass) 65.3% by mass, based on 28.2% by mass of water. And Fujipro F (soy protein manufactured by Fuji Oil Co., Ltd., non-fat solid content 94.3% by mass, protein content 85.8% by mass, lipid content 0.20% by mass) 5.5% by mass was added, and the temperature of the water bath was added. Was stirred while maintaining the temperature at 60 ° C., and after sufficiently dispersing and dissolving, 1% by mass of soymilk oil was added and further stirred, and emulsified into an oil-in-water mold.
After stirring until no lumps of raw materials are observed, homogenize with a pressure homogenizer H20 manufactured by Sanwa Engineering Co., Ltd. at a homogeneous pressure of 20 MPa, heat sterilize at 80 ° C. for 3 minutes with a plate heat exchanger, and then plate. The mixture was cooled to 30 ° C. with a formula heat exchanger to prepare an oil-in-water emulsion having a non-fat solid content content of 9.3% by mass and a fat content of 3.0% by mass.

続いて、この水中油型乳化物をそのままミックス液として使用し、ミックス液100質量部に対し、Lactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis 、Streptococcus thermophilus、Leuconostoc mesenteroides subsp. cremorisの6種を含むフリーズドライタイプの乳酸菌スターターを0.008量部加え、乳酸発酵物A製造の際と同一の発酵条件で発酵を行い、固形分が12.3質量%である乳酸発酵物Cを得た。 Subsequently, this oil-in-water emulsion was used as it was as a mixed solution, and Lactococcus helveticus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. Lacttis biovar diacetylactis, Streptococcus thermophilus were used as they were. , Leuconostoc mesenteroides subsp. Cremoris 6 kinds of freeze-dried type lactic acid bacterium starter was added by 0.008 part, and fermentation was carried out under the same fermentation conditions as in the production of lactic acid fermented product A, and the solid content was 12.3% by mass. Lactococcus fermented product C was obtained.

〔製造例4〕
水89.7質量%に対して、脱脂粉乳(無脂乳固形分95.2質量%、蛋白質含量34質量%)を10.3質量%加えて、水浴の温度を60℃に維持しながら撹拌し、十分に分散・溶解を行った。原料のダマがみられなくなるまで撹拌を行った後、三和エンジニアリング社製圧力式ホモゲナイザーH20型にて均質圧力20MPaで均質化し、プレート式熱交換器にて80℃で3分間加熱殺菌後、プレート式熱交換器にて30℃に冷却し、無脂乳固形分が9.8質量%であり油脂含量が0.1質量%である、乳原料を含むミックス液を調製した。
[Manufacturing Example 4]
10.3% by mass of skim milk powder (non-fat milk solid content 95.2% by mass, protein content 34% by mass) was added to 89.7% by mass of water, and the mixture was stirred while maintaining the temperature of the water bath at 60 ° C. Then, it was sufficiently dispersed and dissolved. After stirring until no lumps of raw materials are observed, homogenize with a pressure homogenizer H20 manufactured by Sanwa Engineering Co., Ltd. at a homogeneous pressure of 20 MPa, heat sterilize at 80 ° C. for 3 minutes with a plate heat exchanger, and then plate. The mixture was cooled to 30 ° C. with a formula heat exchanger to prepare a mixed solution containing a dairy raw material having a non-fat milk solid content of 9.8% by mass and a fat content of 0.1% by mass.

続いて、この乳原料を含むミックス液をそのままミックス液として使用し、ミックス液100質量部に対し、Lactobacillus helveticus、Lactococcus lactis subsp. lactis、Lactococcus lactis subsp. cremoris、Lactococcus lactis subsp. lactis biovar diacetylactis 、Streptococcus thermophilus、Leuconostoc mesenteroides subsp. cremorisの6種を含むフリーズドライタイプの乳酸菌スターター群を0.04質量部加え、乳酸発酵物A製造の際と同一の発酵条件で発酵を行い、固形分が9.9質量%である乳酸発酵物Dを得た。 Subsequently, the mixed solution containing this dairy raw material was used as it was as the mixed solution, and Lactococcus helveticus, Lactococcus lactis subsp. Lactis, Lactococcus lactis subsp. Cremoris, Lactococcus lactis subsp. A freeze-dried type lactic acid bacterium starter group containing 6 types of thermophilus and Leuconostoc mesenteroides subsp. Cremoris was added by 0.04 parts by mass and fermented under the same fermentation conditions as in the production of lactic acid fermented product A, and the solid content was 9.9. Lactococcus fermented product D in mass% was obtained.

<乳清ミネラルの製造>
〔製造例5〕
チーズを製造する際に副産物として得られる甘性ホエーをナノ濾過膜分離した後、更に、逆浸透濾過膜分離により固形分が20質量%となるまで濃縮した。続いて、80℃で20分間の加熱処理をして生じた沈殿を遠心分離して除去し、これを更にエバポレーターで濃縮し、固形分30質量%の乳清ミネラルを得た。得られた乳清ミネラルの固形分中のカルシウム含量は0.4質量%、固形分中の灰分含量は55質量%であった。
<Manufacturing of whey minerals>
[Manufacturing Example 5]
The sweet whey obtained as a by-product in the production of cheese was separated by nanofiltration membrane, and then further concentrated by reverse osmosis filtration membrane separation until the solid content became 20% by mass. Subsequently, the precipitate formed by heat treatment at 80 ° C. for 20 minutes was removed by centrifugation, and further concentrated by an evaporator to obtain whey mineral having a solid content of 30% by mass. The calcium content in the solid content of the obtained whey mineral was 0.4% by mass, and the ash content in the solid content was 55% by mass.

<塩味強化剤の製造>
〔実施例1〕
製造例1で得られた乳酸発酵物Aをそのまま本発明の塩味強化剤Aとした。
〔実施例2〕
製造例2で得られた乳酸発酵物Bをそのまま本発明の塩味強化剤Bとした。
〔比較例1〕
製造例3で得られた乳酸発酵物Cをそのまま比較例1の塩味強化剤Cとした。
〔比較例2〕
製造例4で得られた乳酸発酵物Dをそのまま比較例2の塩味強化剤Dとした。
〔実施例3〕
製造例1で得られた乳酸発酵物Aと製造例5で得られた乳清ミネラルを90:10の質量比で混合・分散し、本発明の塩味強化剤Eとした。
〔実施例4〕
製造例1で得られた乳酸発酵物Aと酵母エキス(ウェルネックスYN−1、富士食品工業製、固形分98質量%粉末品)を75:25の質量比で混合・分散し、本発明の塩味強化剤Fとした。
〔実施例5〕
製造例1で得られた乳酸発酵物Aと製造例5で得られた乳清ミネラルと酵母エキス(ウェルネックスYN−1、富士食品工業製、固形分98質量%粉末品)を72:8:20の質量比で混合・分散し、本発明の塩味強化剤Gとした。
<Manufacturing of salty taste enhancer>
[Example 1]
The lactic acid fermented product A obtained in Production Example 1 was used as it was as the salty taste enhancer A of the present invention.
[Example 2]
The lactic acid fermented product B obtained in Production Example 2 was used as it was as the salty taste enhancer B of the present invention.
[Comparative Example 1]
The lactic acid fermented product C obtained in Production Example 3 was used as it was as the salty taste enhancer C of Comparative Example 1.
[Comparative Example 2]
The lactic acid fermented product D obtained in Production Example 4 was used as it was as the salty taste enhancer D of Comparative Example 2.
[Example 3]
The lactic acid fermented product A obtained in Production Example 1 and the whey mineral obtained in Production Example 5 were mixed and dispersed at a mass ratio of 90:10 to obtain the salty taste enhancer E of the present invention.
[Example 4]
The lactic acid fermented product A obtained in Production Example 1 and yeast extract (Wellnex YN-1, manufactured by Fuji Food Industry Co., Ltd., powdered product having a solid content of 98% by mass) were mixed and dispersed at a mass ratio of 75:25 to obtain the present invention. It was designated as a saltiness enhancer F.
[Example 5]
The lactic acid fermented product A obtained in Production Example 1 and the whey mineral and yeast extract obtained in Production Example 5 (Wellnex YN-1, manufactured by Fuji Food Industry Co., Ltd., powdered product having a solid content of 98% by mass) were combined at 72: 8: It was mixed and dispersed at a mass ratio of 20 to obtain the saltiness enhancer G of the present invention.

<塩味強化剤の評価試験1>
塩化ナトリウム1質量%水溶液を準備した。ここに、上記塩味強化剤Aを、塩化ナトリウム1質量部に対し、固形分がそれぞれ、0.000002質量部、0.00002質量部、0.0002質量部、0.002質量部、0.02質量部、0.2質量部、1質量部、2質量部となるように添加し、十分に分散・溶解し、水性液とした。
(塩味強度及び味質の評価方法)
11人のパネラーに、上記水性液と、対照として用意した塩化ナトリウム1質量%水溶液を舐めさせ、その塩味強度及び味質について、それぞれ下記のパネラーの評価基準により4段階評価させ、その合計点について下記評価基準で5段階又は4段階評価を行なった。その結果をそれぞれ表1、表2に記載した。
<Saltyness enhancer evaluation test 1>
A 1% by mass aqueous solution of sodium chloride was prepared. Here, the salty taste enhancer A has a solid content of 0.000002 parts by mass, 0.00002 parts by mass, 0.0002 parts by mass, 0.002 parts by mass, and 0.02, respectively, with respect to 1 part by mass of sodium chloride. It was added in a mass amount of 0.2 parts by mass, 1 part by mass, and 2 parts by mass, and was sufficiently dispersed and dissolved to prepare an aqueous solution.
(Evaluation method of saltiness strength and taste quality)
Eleven panelists were allowed to lick the above aqueous solution and a 1% by mass aqueous solution of sodium chloride prepared as a control, and the salty taste intensity and taste quality were evaluated on a 4-point scale according to the following panelist's evaluation criteria, and the total score was obtained. A 5-grade or 4-grade evaluation was performed according to the following evaluation criteria. The results are shown in Tables 1 and 2, respectively.

・パネラーの塩味強度評価基準
対照に比べあきらかに強化された塩味を感じる・・ 2点
対照に比べ若干強化された塩味を感じる・・・・・ 1点
対照とほぼ同じ程度の塩味を感じる・・・・・・・ 0点
対照より弱い塩味を感じる・・・・・・・・・・ −1点
・塩味強度評価基準
◎ :11人のパネラーの合計点が17〜22点
○ :11人のパネラーの合計点が11〜16点
△ :11人のパネラーの合計点が6〜10点
× :11人のパネラーの合計点が1〜5点
××:11人のパネラーの合計点が0点未満
・ Evaluation criteria for saltiness strength of panelists I feel a clearly enhanced salty taste compared to the control ・ ・ 2 points I feel a slightly enhanced salty taste compared to the control ・ ・ ・ 1 point I feel almost the same salty taste as the control ・ ・・ ・ ・ ・ ・ 0 points I feel a weaker salty taste than the control ・ ・ ・ ・ ・ ・ ・ ・ -1 point ・ Saltiness intensity evaluation criteria ◎: The total score of 11 panelists is 17 to 22 points ○: 11 people Total score of 11 panelists is 11 to 16 points △: Total score of 11 panelists is 6 to 10 points ×: Total score of 11 panelists is 1 to 5 points × ×: Total score of 11 panelists is 0 points Less than

・パネラーの味質評価基準
塩化ナトリウム以外の風味を全く感じない・・・・・・・・2点
塩化ナトリウム以外の風味を感じるが、塩味として違和感がない・・・・1点
塩化ナトリウム以外の風味を感じ、且つ塩味として違和感がある・・・0点
耐えがたい異味を感じる・・・・・・・・・−1点
・味質評価基準
◎:2点のパネラーが一番多かった。
○:1点のパネラーが一番多かった。
△:0点のパネラーが一番多かった。
×:−1点のパネラーが一番多かった。
・ Paneler's taste evaluation criteria I do not feel any flavor other than sodium chloride ・ ・ ・ ・ ・ ・ ・ ・ 2 points I feel a flavor other than sodium chloride, but there is no discomfort as a salty taste ・ ・ ・ ・ 1 point Other than sodium chloride I feel the flavor and the salty taste is uncomfortable ... 0 points I feel an unbearable offensive taste ... -1 point ・ Taste evaluation criteria ◎: 2 points were the most panelists.
◯: The number of panelists with 1 point was the highest.
Δ: The number of panelists with 0 points was the highest.
X: The number of panelists with -1 point was the highest.

Figure 0006853632
Figure 0006853632

Figure 0006853632
Figure 0006853632

表1及び表2の結果より、乳酸発酵物は、塩化ナトリウム1質量部あたりの添加量が、0.00002〜0.2質量部では塩味の増強効果が見られるが、1質量部以上であると塩味の増強効果はみられず、塩味はほぼ塩化ナトリウムと同レベルとなることがわかる。また、0.2質量部以上であると、塩味以外の風味が感じられるようになり、1質量部以上であると、塩味としての違和感が顕れるようになることがわかる。
以上のことから、本発明に係る乳酸発酵物は、適切な量で用いることにより、塩味強化剤として好適に使用できることがわかる。
From the results of Tables 1 and 2, the salty taste-enhancing effect of the lactic acid fermented product is observed when the amount added per 1 part by mass of sodium chloride is 0.00002 to 0.2 parts by mass, but it is 1 part by mass or more. It can be seen that the salty taste is not enhanced and the salty taste is almost the same level as sodium chloride. Further, it can be seen that when the amount is 0.2 parts by mass or more, a flavor other than the salty taste is felt, and when the amount is 1 part by mass or more, a sense of discomfort as the salty taste appears.
From the above, it can be seen that the lactic acid fermented product according to the present invention can be suitably used as a salty taste enhancer by using it in an appropriate amount.

<塩味強化剤の評価試験2>
塩化ナトリウム0.5質量%水溶液を準備した。ここに、上記塩味強化剤A〜Gを、塩化ナトリウム1質量部に対し、塩味強化剤に含まれる乳酸発酵物の固形分が0.002質量部となるように添加し、十分に分散・溶解し、水性液とした。
(塩味強度及び味質の評価方法)
11人のパネラーに、上記水性液と、対照として用意した塩化ナトリウム0.5質量%水溶液を舐めさせ、その塩味強度及び味質について、それぞれ、下記のパネラーの評価基準により4段階評価させ、その合計点について下記評価基準で5段階又は4段階評価を行なった。その結果をそれぞれ表3、表4に記載した。
<Saltyness enhancer evaluation test 2>
A 0.5% by mass aqueous solution of sodium chloride was prepared. The salty taste enhancers A to G are added thereto so that the solid content of the lactic acid fermented product contained in the salty taste enhancer is 0.002 parts by mass with respect to 1 part by mass of sodium chloride, and the saltiness enhancer is sufficiently dispersed and dissolved. Then, it was made into an aqueous solution.
(Evaluation method of saltiness strength and taste quality)
Eleven panelists were allowed to lick the above-mentioned aqueous solution and a 0.5% by mass aqueous solution of sodium chloride prepared as a control, and the salty taste intensity and taste quality were evaluated on a four-point scale according to the following panelist's evaluation criteria. The total score was evaluated on a 5-point or 4-point scale according to the following evaluation criteria. The results are shown in Tables 3 and 4, respectively.

・パネラーの塩味強度評価基準
対照に比べあきらかに強化された塩味を感じる・・ 2点
対照に比べ若干強化された塩味を感じる・・・・・ 1点
対照とほぼ同じ程度の塩味を感じる・・・・・・・ 0点
対照より弱い塩味を感じる・・・・・・・・・・ −1点
・塩味強度評価基準
◎ :11人のパネラーの合計点が17〜22点
○ :11人のパネラーの合計点が11〜16点
△ :11人のパネラーの合計点が6〜10点
× :11人のパネラーの合計点が1〜5点
××:11人のパネラーの合計点が0点未満
・ Evaluation criteria for saltiness strength of panelists I feel a clearly enhanced salty taste compared to the control ・ ・ 2 points I feel a slightly enhanced salty taste compared to the control ・ ・ ・ 1 point I feel almost the same salty taste as the control ・ ・・ ・ ・ ・ ・ 0 points I feel a weaker salty taste than the control ・ ・ ・ ・ ・ ・ ・ ・ -1 point ・ Saltiness intensity evaluation criteria ◎: The total score of 11 panelists is 17 to 22 points ○: 11 people Total score of 11 panelists is 11 to 16 points △: Total score of 11 panelists is 6 to 10 points ×: Total score of 11 panelists is 1 to 5 points × ×: Total score of 11 panelists is 0 points Less than

・パネラーの味質評価基準
先味を強く感じ、塩化ナトリウムと同等の塩味発現パターンである・・・・・・2点
先味をやや強く感じ、塩化ナトリウムとほぼ同等の塩味発現パターンである・・1点
先味が弱く、塩化ナトリウムと異なる違和感のある塩味発現パターンである・・0点
先味が弱く、塩化ナトリウムと全く異なる塩味発現パターンである・・・・・−1点
・味質評価基準
◎ :2点のパネラーが一番多かった。
○ :1点のパネラーが一番多かった。
△ :0点のパネラーが一番多かった。
×:−1点のパネラーが一番多かった。
・ Paneler's taste evaluation criteria A salty taste expression pattern that feels a strong taste and is equivalent to sodium chloride ..... 2 points A salty taste expression pattern that feels a little strong and is almost the same as sodium chloride.・ 1 point The salty taste expression pattern is weak and different from sodium chloride. ・ ・ 0 point The salty taste expression pattern is weak and completely different from sodium chloride. Evaluation Criteria ◎: The number of panelists with 2 points was the highest.
○: The number of panelists with 1 point was the highest.
Δ: The number of panelists with 0 points was the highest.
X: The number of panelists with -1 point was the highest.

Figure 0006853632
Figure 0006853632

Figure 0006853632
Figure 0006853632

<塩味強化剤の評価試験3>
塩化ナトリウムと塩化カリウムを90:10の質量比とした塩味剤の0.5質量%水溶液を準備した。ここに、上記塩味強化剤A〜Gを、塩味剤1質量部に対し、塩味強化剤に含まれる乳酸発酵物の固形分が0.002質量部となるように添加し、十分に分散・溶解し、水性液とした。尚、塩味強化剤無添加の水性液も用意した。
(塩味強度及び味質の評価方法)
11人のパネラーに、上記水性液と、対照として用意した塩化ナトリウム0.5質量%水溶液を舐めさせ、その塩味強度及び味質について、それぞれ、下記のパネラーの評価基準により4段階評価させ、その合計点について下記評価基準で5段階又は4段階評価を行なった。その結果をそれぞれ表5、表6に記載した。
<Saltyness enhancer evaluation test 3>
A 0.5% by mass aqueous solution of a salting agent having a mass ratio of sodium chloride and potassium chloride of 90:10 was prepared. The salty taste enhancers A to G are added thereto so that the solid content of the lactic acid fermented product contained in the salty taste enhancer is 0.002 parts by mass with respect to 1 part by mass of the salty agent, and the salty taste enhancer is sufficiently dispersed and dissolved. Then, it was made into an aqueous solution. An aqueous solution to which no salty taste enhancer was added was also prepared.
(Evaluation method of saltiness strength and taste quality)
Eleven panelists were allowed to lick the above-mentioned aqueous solution and a 0.5% by mass aqueous solution of sodium chloride prepared as a control, and the salty taste intensity and taste quality were evaluated on a four-point scale according to the following panelist's evaluation criteria. The total score was evaluated on a 5-point or 4-point scale according to the following evaluation criteria. The results are shown in Tables 5 and 6, respectively.

・パネラーの塩味強度評価基準
対照に比べあきらかに強化された塩味を感じる・・ 2点
対照に比べ若干強化された塩味を感じる・・・・・ 1点
対照とほぼ同じ程度の塩味を感じる・・・・・・・ 0点
対照より弱い塩味を感じる・・・・・・・・・・ −1点
・塩味強度評価基準
◎ :11人のパネラーの合計点が17〜22点
○ :11人のパネラーの合計点が11〜16点
△ :11人のパネラーの合計点が6〜10点
× :11人のパネラーの合計点が1〜5点
××:11人のパネラーの合計点が0点未満
・ Evaluation criteria for saltiness strength of panelists I feel a clearly enhanced salty taste compared to the control ・ ・ 2 points I feel a slightly enhanced salty taste compared to the control ・ ・ ・ 1 point I feel almost the same salty taste as the control ・ ・・ ・ ・ ・ ・ 0 points I feel a weaker salty taste than the control ・ ・ ・ ・ ・ ・ ・ ・ -1 point ・ Saltiness intensity evaluation criteria ◎: The total score of 11 panelists is 17 to 22 points ○: 11 people Total score of 11 panelists is 11 to 16 points △: Total score of 11 panelists is 6 to 10 points ×: Total score of 11 panelists is 1 to 5 points × ×: Total score of 11 panelists is 0 points Less than

・パネラーの味質評価基準
塩化ナトリウムと同等の塩味発現パターンである・・・・・・・・2点
塩化ナトリウムとほぼ同等の塩味発現パターンである・・・・1点
塩化ナトリウムと異なる違和感のある塩味発現パターンである・・・0点
塩化ナトリウムと全く異なる塩味発現パターンである・・・・・・・・−1点
・味質評価基準
◎ :2点のパネラーが一番多かった。
○ :1点のパネラーが一番多かった。
△ :0点のパネラーが一番多かった。
×:−1点のパネラーが一番多かった。
・ Paneler's taste evaluation criteria Salty taste expression pattern equivalent to sodium chloride ・ ・ ・ ・ ・ ・ ・ ・ 2 points Salty taste expression pattern almost equivalent to sodium chloride ・ ・ ・ ・ 1 point Discomfort different from sodium chloride A certain salty taste expression pattern ... 0 points A salty taste expression pattern completely different from sodium chloride ... -1 point-Taste evaluation criteria ◎: 2 points were the most common panelists.
○: The number of panelists with 1 point was the highest.
Δ: The number of panelists with 0 points was the highest.
X: The number of panelists with -1 point was the highest.

Figure 0006853632
Figure 0006853632

Figure 0006853632
Figure 0006853632

<飲食品の製造>
〔実施例6〕
水1Lに対し昆布10g及び鰹削り節20gを用い、常法により一番だし(かつお・昆布だし)を作成した。この一番だし300mlに対し、減塩味噌(タケヤ製:減塩味噌)25gを用い、常法に従って、塩化ナトリウムを0.4質量%含有する味噌汁を作成した。ここに塩味強化剤Bを、乳酸発酵物の添加量が塩化ナトリウム1質量部に対し固形分として0.002質量部となるように添加し、十分に混合した。
60℃に保持した状態でカップで2mLを飲み、その塩味強度及び塩味質について、塩味強化剤無添加の味噌汁と比較したところ、塩味強化剤を添加した味噌汁は、無添加の味噌汁に比べ塩味が強化され、且つ、先味が強く感じられる自然な塩味質であった。
<Manufacturing of food and drink>
[Example 6]
Using 10 g of kelp and 20 g of bonito shavings for 1 L of water, the best dashi (bonito and kelp dashi) was prepared by a conventional method. Using 25 g of low-salt miso (manufactured by Takeya: low-salt miso) with respect to 300 ml of this first soup stock, miso soup containing 0.4% by mass of sodium chloride was prepared according to a conventional method. Here, the salty taste enhancer B was added so that the amount of the lactic acid fermented product added was 0.002 parts by mass as a solid content with respect to 1 part by mass of sodium chloride, and the mixture was sufficiently mixed.
When 2 mL was drunk in a cup while being held at 60 ° C. and the saltiness intensity and saltiness were compared with the miso soup without the salty taste enhancer, the miso soup with the salty taste enhancer had a salty taste as compared with the miso soup without the salty taste enhancer. It was a natural salty taste that was fortified and had a strong first taste.

〔実施例7〕
減塩味噌(宮坂醸造製:神州一味噌 無添加減塩)100g、上白糖80g、清酒50g、及び本みりん(タカラ本みりん)25gを鍋に入れ、ここに塩味強化剤Bを、乳酸発酵物の添加量が塩化ナトリウム1質量部に対し固形分として0.002質量部となるように添加し、弱火で加熱しながらよく混ぜ、冷却し、塩化ナトリウムを3.9質量%含有する練り味噌を作成した(仕上がり重量220g)。
長さ7cm、幅3cm、厚さ1.5cmに切った木綿豆腐を火で炙って焼いた後、練り味噌2gを豆腐の両面に塗り、更に軽く火で炙って豆腐田楽を作成した。
この豆腐田楽の両端を除く長さ2cm分(幅3cm、厚さ1.5cm)を喫食し、その塩味強度及び塩味質について、塩味強化剤無添加の豆腐田楽と比較したところ、塩味強化剤を添加した豆腐田楽は、無添加の豆腐田楽に比べ塩味が強化され、且つ、先味が強く感じられる自然な塩味質であった。
[Example 7]
100 g of low-salt miso (manufactured by Miyasaka Brewery: Shinshu Ichimiso additive-free low-salt), 80 g of fine white sugar, 50 g of sake, and 25 g of hon mirin (Takara hon mirin) are put in a pot, and the saltiness enhancer B is added to the lactic acid fermented product. Add soybean paste so that the solid content is 0.002 parts by mass with respect to 1 part by mass of sodium chloride, mix well while heating over low heat, cool, and add 3.9% by mass of sodium chloride to the kneaded miso. Created (finished weight 220 g).
After burning cotton tofu cut into 7 cm in length, 3 cm in width and 1.5 cm in thickness, 2 g of kneaded miso was applied to both sides of the tofu and lightly cooked to make tofu Dengaku.
We ate a length of 2 cm (width 3 cm, thickness 1.5 cm) excluding both ends of this tofu taraku, and compared its saltiness intensity and saltiness with tofu taraku without saltiness enhancer. The added tofu taraku had a more salty taste than the non-added tofu taraku, and had a natural salty taste with a strong first taste.

〔実施例8〕
鍋に水1000mlを入れて火にかけ、水が沸騰したら弱火にし、粉末コンソメ60gを溶かした。ここに塩味強化剤Bを、乳酸発酵物の添加量が塩化ナトリウム1質量部に対し固形分として0.002質量部となるように添加し、弱火で加熱しながらよく混ぜ、冷却し、塩化ナトリウムを0.5質量%含有するコンソメスープを作成した。
60℃に保持した状態でカップで2mLを飲み、その塩味強度及び塩味質について、塩味強化剤無添加のコンソメスープと比較したところ、塩味強化剤を添加したコンソメスープは、無添加のコンソメスープに比べ塩味が強化され、且つ、先味が強く感じられる自然な塩味質であった。
[Example 8]
Put 1000 ml of water in a pan and heat it. When the water boiled, reduce the heat to low and melt 60 g of powdered consomme. Add the salty taste enhancer B so that the amount of the lactic acid fermented product added is 0.002 parts by mass as a solid content with respect to 1 part by mass of sodium chloride, mix well while heating on low heat, cool, and sodium chloride. To prepare a consomme soup containing 0.5% by mass of.
When 2 mL was drunk in a cup while being held at 60 ° C. and the saltiness intensity and saltiness were compared with the consomme soup without the salty taste enhancer, the consomme soup with the salty taste enhancer was converted into the consomme soup without the salty taste enhancer. It was a natural salty taste with a stronger salty taste and a stronger first taste.

〔実施例9〕
減塩醤油(キッコーマン製)に、塩味強化剤Bを、乳酸発酵物の添加量が塩化ナトリウム1質量部に対し固形分として0.002質量部となるように添加し、よく混ぜ塩化ナトリウムを8質量%含有する減塩醤油を作成した。
その塩味強度及び塩味質について、塩味強化剤無添加の減塩醤油と比較したところ、塩味強化剤を添加した減塩醤油は、無添加の減塩醤油に比べ塩味が強化され、且つ、先味が強く感じられる自然な塩味質であった。
[Example 9]
Add salty taste enhancer B to low-salt soy sauce (manufactured by Kikkoman) so that the amount of lactic acid fermented product added is 0.002 parts by mass as a solid content with respect to 1 part by mass of sodium chloride, and mix well to add 8 sodium chloride. A low-salt soy sauce containing% by mass was prepared.
When the saltiness intensity and saltiness were compared with the low-salt soy sauce without the salty taste enhancer, the low-salt soybean oil with the added salty taste enhancer had a stronger salty taste and the first taste than the low-salt soybean oil without the additive-free salty taste enhancer. It was a natural salty taste that was strongly felt.

Claims (7)

乳原料を含む水中油型乳化物を基質とした乳酸発酵物を有効成分とする塩味強化剤であって、塩味剤1質量部に対し、塩味強化剤中の上記乳酸発酵物の固形分が0.00001〜0.004質量部となる量で用いられる塩味強剤。 A salty taste enhancer containing a lactic acid fermented product containing an oil-in-water emulsion containing a dairy raw material as a substrate, and the solid content of the lactic acid fermented product in the salty taste enhancer is 0 with respect to 1 part by mass of the salty taste agent. salty strengthening agent used in an amount of .00001~0.004 parts by mass. 上記乳酸発酵物が、Leuconostoc mesenteroides subsp. cremorisを使用して乳酸発酵した乳酸発酵物である、請求項1に記載の塩味強剤。 The lactic acid fermentation product is, Leuconostoc mesenteroides subsp. Lactic acid fermentations were lactic acid fermentation using cremoris, salty strengthening agent according to claim 1. 上記乳原料が、濃縮乳、練乳、ホエイ、クリーム、バター、バタークリーム、クリームチーズ、ナチュラルチーズ、プロセスチーズ、バターオイル、乳脂分解物、脱脂粉乳、全粉乳、ホエイパウダー、トータルミルクプロテイン、脱脂乳及び脱脂濃縮乳から選択される1又は2以上を含有する、請求項1又は2に記載の塩味強剤。 The above dairy ingredients are concentrated milk, condensed milk, whey, cream, butter, butter cream, cream cheese, natural cheese, process cheese, butter oil, milk fat decomposition products, skim milk powder, whole milk powder, whey powder, total milk protein, skim milk. and containing one or more selected from the defatted concentrated milk, salty strengthening agent according to claim 1 or 2. 更に乳清ミネラルを含有する請求項1〜3の何れか1項に記載の塩味強化剤。 The salty taste enhancer according to any one of claims 1 to 3, further containing whey mineral. 更にエキス類を含有する請求項1〜4の何れか1項記載の塩味強化剤。 The salty taste enhancer according to any one of claims 1 to 4, further containing extracts. 塩味剤を含有する飲食品において、乳原料を含む水中油型乳化物を基質とした乳酸発酵物を有効成分とする塩味強化剤を用いる、飲食品の製造方法であって、
飲食品中の塩味剤1質量部に対し、上記塩味強化剤中の上記乳酸発酵物の固形分が0.00001〜0.004質量部となる量で上記塩味強化剤を飲食品に添加する、飲食品の製造方法
A method for producing a food or drink, which uses a salty taste enhancer containing a lactic acid fermented product as a substrate of an oil-in-water emulsion containing a dairy raw material as an active ingredient in a food or drink containing a salty agent.
The salty taste enhancer is added to the food or drink in an amount such that the solid content of the lactic acid fermented product in the salty taste enhancer is 0.00001 to 0.004 parts by mass with respect to 1 part by mass of the salty taste agent in the food or drink. How to make food and drink .
減塩飲食品を製造する請求項6記載の飲食品の製造方法
The method for producing a food or drink according to claim 6, wherein the food or drink with reduced salt is produced .
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