JP6840647B2 - Liquid composition, its cloudiness improving agent, odor improving agent, and manufacturing method - Google Patents

Description

The present invention relates to a liquid composition, an agent for improving cloudiness thereof, an agent for improving odor, and a method for producing the liquid composition.

Chondroitin is used in many functional foods as a joint care material that smoothes the bending and stretching of the knee joint. Chondroitin is a component that constitutes cartilage, has high water absorption, and has the function of enhancing the cushioning property of the knee joint.

As a prescription form of a functional food containing chondroitin, an emphasis is placed on easy intake before and after exercise, and a beverage is preferable. In addition, since there is a general tendency to ingest a refreshing beverage during exercise, a sour flavor is preferable.

However, the solution containing chondroitin becomes cloudy when it contains an acidulant at the same time, and becomes cloudy when cooled, so that it is difficult to provide it as a beverage. Furthermore, since the chondroitin-containing shark cartilage extract has a peculiar fishy odor, there is a problem that it is difficult to be accepted as a beverage.

The methods described in Patent Documents 1 to 3 are known for suppressing white turbidity of a solution containing chondroitin. The methods described in Patent Documents 4 and 5 are known for suppressing the fishy odor.

Japanese Patent No. 5442510 Japanese Unexamined Patent Publication No. 1-224321 Japanese Unexamined Patent Publication No. 2005-330271 Japanese Unexamined Patent Publication No. 2016-214215 Japanese Patent No. 6075345

An object of the present invention is to provide a chondroitin-containing liquid composition that suppresses the generation of cloudiness and improves the fishy odor.

As a result of diligent studies on the above problems, the present inventors have combined chondroitin with 3-hydroxyisovaleric acid and / or a salt thereof (hereinafter, also collectively referred to as "HMB") to make chondroitin-derived cloudiness. It was discovered that it suppresses the outbreak and improves the fishy odor derived from chondroitin. Based on this discovery, the present inventors have prepared a liquid composition containing (A) component: chondroitin, and (B) component: 3-hydroxyisovaleric acid and / or a salt thereof (hereinafter collectively referred to as "HMB"). It has been found that the above-mentioned problems can be solved by further containing (also referred to as), and the present invention has been completed.

In addition, HMB also has an aspect of being a muscle care food effective in increasing muscle mass and strength. Then, by combining HMB and chondroitin, not only the above-mentioned problems can be solved, but also joints and muscles can be cared for with one product, which is beneficial for consumers.

That is, the present inventors provide the following [1] to [11].
[1] A liquid composition containing (A) component: chondroitin; and (B) component: 3-hydroxyisovaleric acid and / or a salt thereof.
[2] The liquid composition according to the above [1], wherein the component (A) is chondroitin derived from a chondroitin-containing shark cartilage extract.
[3] The liquid composition according to the above [1] or [2], which has a pH of 2 to 4.
[4] The liquid composition according to any one of the above [1] to [3], wherein the content of the component (B) / the content (mass ratio) of the component (A) is 5 to 30.
[5] A beverage composition containing the liquid composition according to any one of the above [1] to [4].
[6] A white turbidity improving agent for a chondroitin-containing liquid composition containing 3-hydroxyisovaleric acid and / or a salt thereof.
[7] The white turbidity improving agent according to the above [6], wherein the chondroitin is a chondroitin derived from a chondroitin-containing shark cartilage extract.
[8] An odor improver for a liquid composition containing a chondroitin-containing shark cartilage extract, which contains 3-hydroxyisovaleric acid and / or a salt thereof.
[9] A method for producing a liquid composition containing chondroitin with improved white turbidity, which comprises a step of mixing chondroitin with 3-hydroxyisovaleric acid and / or a salt thereof.
[10] The production method according to the above [9], wherein the chondroitin is a chondroitin derived from a chondroitin-containing shark cartilage extract.
[11] A method for producing a liquid composition containing a chondroitin-containing shark cartilage extract having an improved odor, wherein the chondroitin-containing shark cartilage extract is mixed with 3-hydroxyisovaleric acid and / or a salt thereof. Manufacturing method, including steps.

According to the present invention, it is possible to provide a chondroitin-containing liquid composition in which the occurrence of cloudiness is suppressed and the fishy odor is improved. Furthermore, by making the liquid composition into a beverage composition, it is possible to provide care with one product of joints and muscles.

Hereinafter, the present invention will be described in detail according to the preferred embodiment thereof.

[Liquid composition]
In one embodiment, the present invention provides a liquid composition.

The liquid composition of the present invention
(A) component: chondroitin; and (B) component: 3-hydroxyisovaleric acid and / or a salt thereof.

((A) component: chondroitin)
Chondroitin is widely distributed in the human body as chondroitin sulfate, and since it is contained in a large amount in the cartilage and skin of joints, it is used as a health food for the purpose of improving osteoarthritis and beauty of the skin. It's being used. The chondroitin used as the component (A) of the liquid composition of the present invention includes chondroitin, which is a kind of mucopolysaccharide composed of hexosamine and uronic acid, chondroitin sulfate which is a sulfate ester thereof, and a physiologically acceptable salt thereof (the chondroitin sulfate). For example, sodium salt). Chondroitin is abundantly contained in the cornea, crystalline lens, ciliary muscle, vitreous body, cartilage, bone, tendon, blood vessel wall, skin and the like in a state of being bound to a protein, and a chondroitin-protein complex can also be used.
Chondroitin can be obtained from animal cartilage and the like, but chemically synthesized ones or mixtures thereof may be used. In addition to purified chondroitin, for example, cartilage powder or cartilage extract can also be used as long as it contains chondroitin as a component.

Examples of cartilage containing a high concentration of chondroitin include shark cartilage, whale cartilage, squid cartilage, salmon cartilage, chicken cartilage, pig cartilage, and bovine cartilage. Of these, shark cartilage is preferred. The shark cartilage has no limitation on the type of shark from which it is derived, and examples thereof include spiny dogfish and blue shark. Further, there is no limitation on the origin of cartilage, and examples thereof include head and fins.

Examples of the raw material derived from shark cartilage include shark cartilage powder and shark cartilage extract. The chondroitin-containing shark cartilage extract is widely used as a health food material containing a high concentration of chondroitin, and is one of the preferred forms of the component (A) of the liquid composition of the present invention.
The chondroitin-containing shark cartilage extract is, for example, shredded shark cartilage, treated with a proteolytic enzyme in a solvent such as water, extracted, purified by a method such as adsorption or filtration, and an excipient such as dextrin is used. In addition, it may be powdered by a method such as spray drying. The main component of the chondroitin-containing shark cartilage extract is sodium chondroitin sulfate, which is a polysaccharide having a molecular weight of about 100,000 to several hundred thousand.

As the chondroitin, a commercially available product may be used. For example, under the trade names, chondroitin sulfate 20, chondroitin sulfate 40 (all manufactured by Toyo Hakko Co., Ltd.), SCP, SCP (NB), SCP Super Fine 70, SNC-20N, SNC-40N, salmon PG-20, salmon PG, Squid-CS20, SC20 (above, manufactured by Maruhanichiro Foods), pig chondroitin 20FF, pig chondroitin 40FF, pig chondroitin 20HJ, sodium chondroitin sulfate (above, manufactured by Japan Biocon), shark-derived chondroitin sulfate CP-55 (Biocorp Japan) , SCS-20, SCS-40 (above, manufactured by Yaegaki Ferment Giken Co., Ltd.), Marlinki Ridge S, Marin Rinti Ridge 40S (above, manufactured by YSK Chondroitin Sulfate Chemical Industry Co., Ltd.), MC Chondroitin Sulfate Protein Complex, SCS Chondroitin Na Sulfate (above, made by Japan Barrier Free), Chondroitin Sulfate A Solute, Chondroitin Sulfate B Sodium Salt, Chondroitin Sulfate C Sodium, Chondroitin Sulfate Sodium Sulfate (above, Chondroitin Sulfate), Chondroitin Sulfate (Genuine Chemical) , CAS No. 9082-07-9), Chondroitin Sulfate Sodium (GK) (manufactured by Biochemical Industry Co., Ltd.), Chondroitin HG-20 for shark chondroitin-derived foods, Chondroitin HG-40 for shark chondroitin-derived foods, Chondroitin HG-60 for foods derived from shark cartilage, Chondroitin HG-70 for foods derived from shark cartilage (above, manufactured by Nippon Yakuhin Co., Ltd.), Chondroitin Q20%, Chondroitin Q40% (above, manufactured by Cupy), P-chondroitin, P-chondroitin Examples include NHZ (manufactured by Nippon Ham Co., Ltd.), Chondroitin Sulfate Saudium Salt (manufactured by Tokyo Kasei Kogyo Co., Ltd.), and chondroitin sulfate C sodium sulfate (manufactured by Sigma Aldrich).

The component (A) may be chondroitin sulfate, chondroitin contained in a chondroitin-containing shark cartilage extract, or a mixture thereof. The content of the component (A) can be determined as a guideline that the intake of sodium chondroitin sulfate is 0.2 to 600 mg, preferably 10 to 200 mg per adult per day. The content of chondroitin, for example, in the case of sodium chondroitin sulfate, is usually 0.00004 to 12% by mass, preferably 0.002 to 4% by mass, based on the total mass of the liquid composition.
When chondroitin contained in chondroitin-containing shark cartilage extract is used as the component (A), for example, in the case of chondroitin-containing shark cartilage extract containing 20% chondroitin (for example, SC20 manufactured by Maruhanichiro Foods Co., Ltd.), the intake amount. The daily dose per adult can be determined to be 1 to 3000 mg, preferably 50 to 1000 mg per day. For example, in the case of SC20 manufactured by Maruha Nichiro Foods Co., Ltd., the chondroitin content is usually 0.0002 to 60% by mass, preferably 0.01 to 20% by mass, based on the total mass of the liquid composition.
When a mixture of chondroitin sulfate and chondroitin contained in the chondroitin-containing shark cartilage extract is used as the component (A), the content of sodium chondroitin sulfate may be as described above.

(Component (B): 3-hydroxyisovaleric acid and / or a salt thereof)
The component (B) is 3-hydroxyisovaleric acid (3-Hydroxy-3-Methyl Butyrate, β-Hydroxy-β-Methylbutyrate) and / or a salt thereof, and is a metabolite of leucine. It is also used as a muscle care material that is effective in increasing muscle mass and strength, and is classified as a food.

The salt of 3-hydroxyisovaleric acid may be any salt that is acceptable as food. For example, inorganic acid salts such as hydrochloride, hydrobromide, sulfate, hydroiodide, nitrate, phosphate; citrate, oxalate, acetate, formate, propionate, benzoate. Organic acid salts such as acid salts, trifluoroacetates, maleates, tartrates, methanesulfonates, benzenesulfonates, or paratoluenesulfonates; sodium salts, potassium salts, calcium salts, magnesium salts, ammonium Inorganic base salts such as salts; organic base salts such as triethylammonium salt, triethanolammonium salt, pyridinium salt and diisopropylammonium salt; amino acid salts such as arginine, aspartic acid and glutamic acid. Among them, an inorganic base salt is preferable, and a calcium salt is more preferable.

The HMB may be derived from a natural product, artificially produced, genetically modified, or commercially available. Further, HMB may be a combination of two or more obtained by various acquisition methods.
As the HMB, a commercially available product may be used. Examples of commercially available products include product names HMB Kyowa (manufactured by Kyowa Hakko Bio Co., Ltd.), Kobayashi HMB powder (manufactured by Kobayashi Fragrance Co., Ltd.), β-Hydroxyisovaleric Acid (manufactured by Tokyo Chemical Industry Co., Ltd.), and β-hydroxyisovaleric acid (Sigma. (Manufactured by Aldrich), β-hydroxyisovaleric acid (manufactured by Wako Pure Chemical Industries, Ltd.).

The content of the component (B) is determined based on the standard that the intake of HMB is 0.1 to 10 g, preferably 0.5 to 5 g, and more preferably 1.5 to 3 g per day per adult. be able to.
The content of the component (B) is usually 0.01 to 25% by mass, preferably 0.30 to 20.0% by mass, based on the total mass of the liquid composition.
When the component (A) is sodium chondroitin sulfate, the mass ratio of the component (B) to the component (A) ((B) / (A)) is 25 or more because white turbidity does not occur even at 4 ° C. It is preferable, and since white turbidity does not occur, it is preferably 150 or less, preferably 25 to 150, and more preferably 25 to 50.
When the component (A) is a chondroitin-containing shark cartilage extract, the mass ratio of the component (B) to the component (A) ((B) / (A)) does not cause cloudiness even at 4 ° C. and has a fishy odor. Since the suppression is good, it is preferably 5 or more, and since white turbidity does not occur and the fishy odor is well suppressed, it is preferably 30 or less, preferably 5 to 30, and more preferably 5 to 10.
When a mixture of chondroitin sulfate and chondroitin contained in the chondroitin-containing shark cartilage extract is used as the component (A), the mass ratio of the component (B) to the component (A) ((B) / (A)) is , Chondroitin sodium sulfate may have the above-mentioned mass ratio.

(Arbitrary ingredient)
The liquid composition of the present invention may contain the above-mentioned components as active ingredients, and may contain components other than the above-mentioned components. Examples of components other than the above include pharmacologically acceptable bases. Examples of pharmacologically acceptable bases include components that primarily ensure stability in storage and distribution (eg, storage stabilizers, etc.). In addition, one or two or more kinds of ingredients (preferably about 1 to 3 kinds, more preferably about 1 kind) selected from various ingredients constituting the target final product (for example, food and drink, pharmaceutical products, quasi-drugs, etc.). ) May be contained.

The liquid composition of the present invention may contain other optional components as long as the effects and functions of the present invention are not impaired. For example, sweeteners such as sucrose, sugar, honey, glucose fructose liquid sugar, glucose, licorice (glycyrrhizin), fructose, somatin, malt sugar, brown sugar, aspartame; citric acid, acetic acid, lactic acid, propionic acid, sorbic acid, butyric acid, Organic food-grade acids such as formic acid, fumaric acid, malic acid, tartaric acid, succinic acid, and gluconic acid (which also function as acidity-imparting agents) and sodium salts such as citric acid, lactate, malate, and succinate. , Calcium salt, potassium salt, carbonate and other pH adjusters; dibutylhydroxytoluene, butylhydroxyanisole, erythorbic acid, EDTA, sodium sulfite, sodium hydrogen sulfite and other synthetic antioxidants; carotenoids, lycopene, lutein, zeaxanthin and the like Carotenoids; antioxidant vitamins such as vitamin A, vitamin B, vitamin C (ascorbic acid), vitamin E (tocopherol, tocotrienol); flavonoids such as catechin, isoflavone; polyphenols or derivatives thereof, rosemary extract, etc. Antioxidants; Medicinal ingredients such as aloe juice, Amachazuru, medicated carrots; Fruit juices such as apple juice, plum juice, orange juice, grapefruit juice; , Carotenoid pigments, edible tar pigments, Benikouji pigments, Benibana red pigments, Benibana chlorophyll, and other colorants. The liquid composition of the present invention preferably contains an acidity-imparting agent because it gives a refreshing sensation when ingested as a beverage composition.

(PH)
The liquid composition of the present invention is preferably acidic because it gives a refreshing sensation when ingested as a beverage composition. The pH of the liquid composition of the present invention is preferably 2.0 to 4.0, more preferably 3.0 to 4.0. For the pH, for example, the above-mentioned pH adjuster, the above-mentioned organic acid for food, hydrochloric acid, sulfuric acid, carbonic acid, phosphoric acid, nitric acid and other inorganic acids, sodium hydroxide, potassium hydroxide, calcium hydroxide and other bases are used. It can be adjusted to a desired value.

(Use of liquid composition)
The liquid composition of the present invention can be used as a pharmaceutical product, a quasi drug, or a food product, and is preferably used as a food product. When the liquid composition of the present invention is used as a food, it can be used in the form of a beverage composition.

The liquid composition of the present invention can also be used as a final product in its original form. Further, it can be used as an additive for foods and drinks, an additive for pharmaceuticals, and an additive for quasi-drugs. As a result, various effects can be imparted to foods and drinks, pharmaceuticals, and quasi-drugs.

The pharmacologically acceptable base is not particularly limited as long as the object of the present invention is not impaired. For example, colorants, color formers, flavoring agents, flavoring agents, antioxidants, preservatives, flavoring agents, acidulants, sweeteners, enhancers, vitamins, swelling agents, thickeners, surfactants, etc. One or more of the products that do not impair the properties required for the formulation (eg, formulation stability) and that correspond to the dosage form of the final product (eg, pharmaceuticals, quasi-drugs, foods and drinks). Two or more types can be selected.

When the liquid composition of the present invention contains a pharmacologically acceptable base material, the blending amount of the active ingredient (in the case of a combination of two or more kinds of ingredients, the total amount) is particularly effective as long as it is an effective amount. It is not limited, but is usually in the range of 0.1 to 30% by mass.

The administration form of the liquid composition of the present invention is not particularly limited. Examples include oral administration (eg, oral administration, sublingual administration, etc.), parenteral administration (eg, intravenous administration, intramuscular administration, subcutaneous administration, transdermal administration, nasal administration, pulmonary administration, etc.). Be done. Among these, a less invasive administration form is preferable, and oral administration (oral administration) is more preferable.

The dosage form of the liquid composition of the present invention can be appropriately determined depending on whether it is a food or drink, a pharmaceutical product, or a quasi-drug, and is not particularly limited. Examples of dosage forms for oral administration include liquid (liquid), syrup (syrup), capsule (capsule), soft capsule (soft capsule), semi-liquid, cream, paste, etc. Can be mentioned.

The subject of ingestion of the liquid composition of the present invention is not particularly limited. Examples include those with locomotive syndrome, sarcopenia, and frailty, but they do not need to be diagnosed by a doctor or medical institution as having a particular illness. Targets who may fall under locomotive syndrome, sarcopenia, frailty, etc., those who do not want to be in that state, and those who are considered by a third party not to be in that state. May be good. Subjects include, for example, those who feel that at least one of their muscles, bones and joints has weakened, those whose relatives have locomotive syndrome, or sarcopenia, frailty, the elderly, muscles, bones and Examples include subjects who wish to prevent or ameliorate the decline of at least one of the joints. Even subjects who do not have any particular anxiety can take it on a daily basis for the purpose of preventing locomotive syndrome, sarcopenia, and frailty.

The definition of locomotive syndrome proposed by the Japanese Orthopedic Association in 2007 is that there is a high risk of requiring nursing care due to a disorder of the locomotive organs. Musculoskeletal disorders are interpreted as a defect in at least one of the muscles, joints and bones or a loss of balance. It is said that as the locomotive syndrome progresses, it will eventually become bedridden and require long-term care.

Sarcopenia means a state in which skeletal muscle mass and skeletal muscle strength that occur with aging decrease. Suppressing muscle weakness is also very important in the prevention of locomotive syndrome. When the overall function of living is lower than that of sarcopenia, it is called frail.

The administration time of the composition of the present invention is not particularly limited, but it is more preferable before and after exercise or meal.

The liquid composition of the present invention includes health foods, functional foods, nutritional supplements (supplements), foods for specified health use, medical foods, foods for the sick, infant foods, nursing foods, foods for the elderly, etc. It may be used as food.

(Beverage composition)
In one embodiment, the liquid composition of the present invention can be used as a beverage composition. The beverage composition of the present invention contains the liquid composition of the present invention, and may further contain an optional component. Examples of the optional component include those described above.

[White turbidity improver]
In one embodiment, the present invention provides a white turbidity improving agent for a liquid composition containing component (A): chondroitin. The cloudiness improving agent of the present invention contains the component (B): 3-hydroxyisovaleric acid and / or a salt thereof. As described above, the liquid composition containing chondroitin has a problem that it becomes cloudy especially when it contains an acidulant at the same time, and further cloudiness increases when it is cooled. However, 3-hydroxyisovaleric acid and / or its Since the white turbidity is suppressed by the addition of the salt, the agent of the present invention has the effect of improving the white turbidity.

The cloudiness improving agent of the present invention can also be used, for example, when the component (A) is the above-mentioned component. In particular, the cloudiness improving agent of the present invention can also be used when the component (A) is chondroitin contained in a chondroitin-containing shark cartilage extract.

Regarding the component (B) of the white turbidity improving agent of the present invention, the type of salt of 3-hydroxyisovaleric acid, the origin of HMB, the content of HMB, and the mass ratio of HMB to the component (A) ((B) / (A)). ) May be selected from, for example, those described above.

[Odor improver]
In one embodiment, the present invention provides an odor improver in a liquid composition containing component (A): chondroitin-containing shark cartilage extract. The odor improving agent of the present invention contains the component (B): 3-hydroxyisovaleric acid and / or a salt thereof. As described above, the chondroitin-containing shark ointment extract has a problem that it has a peculiar fishy odor. However, since the fishy odor is suppressed by the addition of 3-hydroxyisovaleric acid and / or a salt thereof, the agent of the present invention. Has an odor improving effect.

Regarding the component (B) of the odor improving agent of the present invention, the type of salt of 3-hydroxyisovaleric acid, the origin of HMB, the content of HMB, and the mass ratio of HMB to the component (A) ((B) / (A)). ) May be selected from, for example, those described above.

[Manufacturing method of liquid composition with improved white turbidity]
In one embodiment, the present invention provides a method for producing a liquid composition containing (A) component: chondroitin, which has improved cloudiness. The above manufacturing method is
(A) Ingredients: Chondroitin and
(B) Ingredient: Including the step of mixing 3-hydroxyisovaleric acid and / or a salt thereof. By mixing the component (B): 3-hydroxyisovaleric acid and / or a salt thereof, the occurrence of white turbidity derived from chondroitin can be improved, and a liquid composition having improved white turbidity can be produced.

The above-mentioned production method can also be used, for example, when the component (A) is the above-mentioned component. In particular, the above production method can also be used when the component (A) is chondroitin contained in a chondroitin-containing shark cartilage extract.

Regarding the component (B) used in the above production method, the type of salt of 3-hydroxyisovaleric acid, the origin of HMB, the content of HMB, and the mass ratio of HMB to the component (A) ((B) / (A)) are , For example, you may choose from those mentioned above.

[Manufacturing method of liquid composition with improved odor]
In one embodiment, the present invention provides a method for producing a liquid composition containing a component (A): chondroitin-containing shark cartilage extract with improved odor. The above manufacturing method is
(A) Ingredients: Chondroitin-containing shark cartilage extract and
(B) Ingredient: Including the step of mixing 3-hydroxyisovaleric acid and / or a salt thereof. (B) Ingredient: By mixing 3-hydroxyisovaleric acid and / or a salt thereof, the fishy odor derived from the chondroitin-containing shark cartilage extract can be improved, and a liquid composition having an improved odor is produced. be able to.

Regarding the component (B) used in the above production method, the type of salt of 3-hydroxyisovaleric acid, the origin of HMB, the content of HMB, and the mass ratio of HMB to the component (A) ((B) / (A)) are , For example, you may choose from those mentioned above.

Hereinafter, the present invention will be described in more detail based on Examples. However, the present invention is not limited to the following examples.

(Evaluation methods)
・ White turbidity evaluation (transmittance evaluation)
Using UV1800 (manufactured by Shimadzu Corporation) as a spectrophotometer, the transmittance of the liquid composition sample at 600 nm was measured.

・ White turbidity evaluation (visual evaluation)
A 10 mL liquid composition sample was placed in a 20 mL transparent glass bottle with a screw cap, and the degree of cloudiness was visually evaluated. The evaluation criteria are shown below.
⊚: Not cloudy ○: Almost not cloudy △: Slightly cloudy ×: Cloudy

-Evaluation of fishy odor 10 mL of the liquid composition sample was placed in a 20 mL glass bottle with a screw cap, and 5 panelists, male and female, smelled the odor and performed a sensory evaluation according to the following scores. The evaluation criteria are shown below.
<Score>
5 points: Very good with almost no fishy odor.
4 points: Good with a slight fishy odor.
3 points: A slightly fishy odor is felt.
2 points: You can feel the fishy smell.
1 point: It has a strong fishy odor and is difficult to eat and drink.
<Evaluation criteria>
◎: The average score of the five panelists is 4.5 points or more.
◯: The average score of the five panelists is 4 points or more and less than 4.5 points.
Δ: The average score of the five panelists is 3.5 points or more and less than 4 points.
×: The average score of the five panelists is less than 3.5 points.

The ingredients used in the following Examples, Comparative Examples and Formulation Examples are shown below.
Chondroitin-containing shark cartilage extract (trade name "SC20", manufactured by Maruha Nichiro Foods Co., Ltd., containing 20% chondroitin sulfate)
HMB Calcium (manufactured by Tokyo Chemical Industry Co., Ltd.)
Dextrin (trade name GLUCIDEX IT 47, manufactured by Rocket Japan)
Malic acid (food additive, manufactured by Wako Pure Chemical Industries, Ltd.)
L-Theanine (manufactured by Wako Pure Chemical Industries, Ltd.)
Chlorogenic acid (manufactured by Wako Pure Chemical Industries, Ltd.)

(Examples 1 to 18 and Comparative Examples 1 to 9)
(Preparation of liquid composition sample)
The amounts of each component shown in Tables 1 to 3 were blended, and the pH was adjusted to 2.0 to 4.0 with dilute hydrochloric acid and / or sodium hydroxide to prepare a liquid composition sample.

(White turbidity evaluation)
The liquid composition sample was placed in a transparent glass bottle with a screw cap, and cloudiness was evaluated immediately after preparation. Then, the mixture was allowed to stand at 4 ° C. for 7 days, and the white turbidity was evaluated again. Since it has been reported that L-theanine suppresses the white turbidity of a solution containing chondroitin (Patent No. 5442510), it was used for evaluation as a benchmark. As shown in Tables 1 to 3, the occurrence of cloudiness was suppressed by increasing the blending amount of HMB. Further, when the mixture was allowed to stand at 4 ° C., the white turbidity tended to be more remarkable in the comparative example, but the worsening of the white turbidity was suppressed by adding HMB.

(Evaluation of fishy odor)
The liquid composition sample was placed in a glass bottle with a screw cap, and the fishy odor was evaluated. Since it has been reported that chlorogenic acid suppresses the odor of animal proteins (Patent No. 3830137), it was used for evaluation as a benchmark. As shown in Tables 1 to 3, the fishy odor was suppressed by increasing the blending amount of HMB.

(Evaluation of results)
Summarizing the above results, by blending HMB with a beverage containing chondroitin having a pH of 2.0 to 4.0, the generation of cloudiness and fishy odor are suppressed, and the inappropriate state as a beverage is improved. It became clear.

(Prescription example)
Table 4 shows a formulation example when the liquid composition of the present invention is used as a beverage composition.

Claims (11)

A liquid composition containing (A) component: chondroitin; and (B) component: 3-hydroxyisovaleric acid and / or a salt thereof. The liquid composition according to claim 1, wherein the component (A) is chondroitin derived from a chondroitin-containing shark cartilage extract. The liquid composition according to claim 1 or 2, wherein the pH is 2 to 4. The liquid composition according to any one of claims 1 to 3, wherein the content of the component (B) / the content (mass ratio) of the component (A) is 5 to 30. A beverage composition comprising the liquid composition according to any one of claims 1 to 4. A white turbidity improving agent for a chondroitin-containing liquid composition containing 3-hydroxyisovaleric acid and / or a salt thereof. The white turbidity improving agent according to claim 6, wherein the chondroitin is a chondroitin derived from a chondroitin-containing shark cartilage extract. An odor improver for a chondroitin-containing shark cartilage extract-containing liquid composition containing 3-hydroxyisovaleric acid and / or a salt thereof. A method for producing a liquid composition containing chondroitin with improved white turbidity, which comprises a step of mixing chondroitin with 3-hydroxyisovaleric acid and / or a salt thereof. The production method according to claim 9, wherein the chondroitin is a chondroitin derived from a chondroitin-containing shark cartilage extract. A method for producing a liquid composition containing a chondroitin-containing shark cartilage extract having an improved odor, which comprises a step of mixing the chondroitin-containing shark cartilage extract with 3-hydroxyisovaleric acid and / or a salt thereof. ,Production method.