JP6827165B2 - Feed composition for boosting immunity of suckling livestock - Google Patents

Description

The present invention relates to a feed composition for enhancing immunity of suckling livestock and the like. More specifically, the present invention relates to a feed composition, a feeding method, and the like for enhancing immunity in suckling livestock (cattle, horses, pigs, sheep, goats, etc.).

In the dairy and livestock industries, the number of deaths of livestock during the feeding period is still high even now that the feeding technology and the livestock medical technology have improved, and there is a demand for a reduction in the mortality rate. For example, with regard to cattle, 10% of calves have died within the first month of life in Hokkaido, and related organizations are currently working together to improve the breeding environment.

In addition, calves are vulnerable to infectious diseases, and it is said that most of the deaths during breeding due to diarrhea and pneumonia occur during the lactation period. In order to reduce the mortality rate of suckling livestock such as calves, it is considered important to improve the breeding environment (especially the sanitary environment) and to improve the health of the individual (especially immune resistance).

Regarding the enhancement of immune resistance (immunity enhancement) of this individual, for example, in the case of calves, immune antibodies (immunoglobulins such as IgG, IgM, IgA) are transferred from mother cows to calves by feeding the first milk. By doing so, you gain immunity. Calves from birth to about 4 to 8 weeks of age are unable to produce antibodies to resist infectious diseases, etc., and are derived from the mother only by ingesting first milk containing a large amount of immune antibodies. Immune antibodies can be obtained, which is called passive immunity. Since the morbidity and mortality rates of calves with deficient passive immunity transfer increase, a method for diagnosing passive immunity deficiency has also been proposed (Non-Patent Document 1).

The mechanism of immune antibody transfer is the same for domestic animals such as horses, pigs, sheep, and goats, and the antibody does not transfer from the mother to the offspring through the placenta of the mother, but is transferred by the first milk. Then, a component having an immunoglobulin activating activity (Patent Document 1) and an immunoglobulin absorption promoting activity (Patent Document 2) of suckling livestock has been found, and by concentrating the primary milk containing IgG. Although high IgG-rich substances are commercially available, comprehensive efforts from various fields such as development of further components capable of enhancing the immunity of suckling livestock have been desired in the industry.

On the other hand, maltitol is a sugar alcohol obtained by high-pressure catalytic reduction (hydrogenation) of high-purity maltose starch syrup (main component: maltose) in the presence of a catalyst, and it has a sweetness similar to sugar. Since it is a resistant sugar, it is widely used in various health foods as a low-calorie material. There is also an example of using maltitol as animal feed (Patent Document 3), but it is desired to further expand its use and expand the market.

However, the effect on immunity and the like when maltitol is fed or orally administered to suckling livestock has not been known so far.

Japanese Unexamined Patent Publication No. 2005-314280 Japanese Unexamined Patent Publication No. 2008-231066 Japanese Unexamined Patent Publication No. 5-52

Journal of Industrial Animal Clinicians, 4 (1): 1-7, 2013

The present invention is a feed composition for enhancing the immunity of feeding livestock (cattle, horses, pigs, sheep, goats, etc.), which is simple, effective and highly safe, using ingredients having abundant eating experience. , The purpose is to provide a method for raising livestock during the feeding period.

In order to achieve the above object, as a result of diligent research, the present inventors enhanced their immunity by feeding or orally administering a feed composition or the like containing martitol as an active ingredient to livestock during the feeding period, thereby enhancing the feeding power. The present invention has been completed by finding that it is possible to reduce the mortality rate of livestock and prevent infectious diseases.

That is, the embodiment of the present invention is as follows.
(1) Feed composition for enhancing immunity of suckling livestock containing Martinol as an active ingredient (containing as an active ingredient) (particularly, feed composition for reducing mortality rate and / or preventing infectious diseases by enhancing immunity) ..
(2) The composition according to (1), wherein the feed composition contains primary milk and / or artificial primary milk.
(3) The composition according to (1) or (2), wherein the enhancement of immunity is the promotion of immunoglobulin absorption.
(4) The composition according to (3), wherein the promotion of immunoglobulin absorption is the promotion of IgG absorption.
(5) The feed composition according to any one of (1) to (4), wherein the livestock is one or more selected from cattle, horses, pigs, sheep, and goats.

(6) A method for enhancing immunity of a suckling livestock, which comprises feeding or orally administering maltitol (for example, a feed composition or a pharmaceutical composition containing maltitol) to the feeding livestock.
(7) A method for reducing mortality and / or preventing infectious diseases by enhancing immunity of suckling livestock, which comprises feeding or orally administering maltitol to feeding livestock.
(8) The method according to (6) or (7), wherein the enhancement of immunity is the promotion of immunoglobulin absorption.
(9) The method according to (8), wherein the promotion of immunoglobulin absorption is the promotion of IgG absorption.
(10) The method according to any one of (6) to (9), which comprises feeding or orally administering the drug within 48 hours after birth.
(11) The method according to any one of (6) to (10), which comprises feeding maltitol mixed with first milk or artificial first milk.
(12) The method according to any one of (6) to (11), wherein the livestock is one or more selected from cattle, horses, pigs, sheep, and goats.
(13) A method for producing milk, which comprises milking from a dairy cow (lactating cow) bred by the method according to any one of (6) to (11) during the lactation period.

According to the present invention, it is possible to easily and effectively enhance the immunity, reduce the mortality rate of the suckling livestock, prevent infectious diseases, etc. simply by feeding or orally administering a component highly safe for the feeding livestock. .. In addition, maltitol, like sugar and starch syrup, is widely used as a raw material for processed foods, and it is easy to use for livestock breeding, which is expected to have the effect of expanding the market due to new uses of maltitol.

Time from calf birth in the test of Example 1 (MAL group: black circle and alternate long and short dash line), control group (white triangle and solid line), and comparison target group (FOS group: black square and dotted line) It is a graph which shows the relationship between the horizontal axis: day) and its serum IgG concentration (vertical axis: g / L). In addition, a and b in the graph indicate that there is a significant difference between different characters (p <0.05). The apparent IgG absorption rate (AEA: Apparent Efficiency of Absorption) of calves in the test group of the present invention (MAL group: right end), control group (left end), and comparison target group (FOS group: center) in the test of Example 1 was determined. It is a graph which shows. In addition, a and b in the graph indicate that there is a significant difference between different characters (p <0.05).

First, in the present invention, maltitol is used as an active ingredient of a feed composition for enhancing immunity of suckling livestock (supplements, pharmaceutical compositions, etc. may be used). Maltitol is a sugar alcohol obtained by a method of reducing (hydrogenating) maltose, and if necessary, dry powder, granules, liquid, paste, etc. can be used, and the use thereof. The form is not particularly limited. Further, the use of not only pure maltitol products (refined products) but also crude products and commercial products is not excluded, but it is preferable to use products refined to a certain content (for example, 10% or more).

Then, the feed composition or the like containing this maltitol as an active ingredient is fed or orally administered to the suckling livestock. For example, in calves, the feeding or oral dose is about 1 to 50 g / head, preferably about 5 to 20 g / head as maltitol per feeding or oral administration twice a day. In particular, it is preferable to administer twice within 12 hours after birth, and to feed or orally administer 20 to 100 g / head or more by 48 hours after birth. This amount is about 160 to 1400 mg per 1 kg of body weight per day. The number of feedings / administrations, the amount of maltitol, etc. can be adjusted as appropriate with reference to the above. For livestock other than calves, the dose may be the same as the dose, or the dose may be set by performing the required conversion based on the body weight or the like from this value. Regarding the start time of feeding or oral administration, it is preferable to start feeding or oral administration within 48 hours after birth, preferably within 36 hours, more preferably within 24 hours, and most preferably within 1 hour. Take as an example. The amount of maltitol may be appropriately adjusted according to the number of feedings (administration).

Examples of livestock to be the subject of the present invention include ruminant livestock (dairy cows, beef cattle, sheep, goats, etc.), horses, pigs, etc., in which milk and meat are used by humans. The present invention is characterized in that the mechanism of immune antibody transfer is passive immunity, and is effective during the feeding period of livestock (for example, in cattle, from the time of birth to about 4 to 8 weeks of age).

Examples of the form of the feed composition and the like according to the present invention include powders, granules, syrups, liquids and the like obtained by mixing colostrum and other feed raw materials into powder, granules, pastes, liquids and the like. Although it may be fed or orally administered as a preparation of the above, it is a feed obtained by mixing multitoll with milk replacer or artificial milk containing a large amount of immunoglobulin (IgG, IgM, IgA, etc.) or milk replacer or artificial milk. It is preferably fed as a composition. In particular, it is preferable to prepare a composition that can supply about 1 to 10 g of immunoglobulin per 1 kg of body weight by 24 hours after birth together with maltitol. In addition, there are no particular restrictions on the combined use of immunity-enhancing components (such as raffinose and DFA) other than maltitol, other functional components, and live lactic acid bacteria. Furthermore, maltitol is separately fed or fed separately. It may be administered orally.

Here, in the present invention, the first milk means milk that is milked for the first time or the second time after delivery, and the artificial first milk means a feed for livestock for livestock having a composition similar to that of the first milk. The milk replacer means a feed containing skim milk powder as a main raw material, dissolved in a liquid form and fed, and the artificial milk means a mixed feed other than the milk replacer fed in a solid form.

In this way, by feeding or orally administering Martinol to the suckling livestock, the immunity thereof can be enhanced, particularly the promotion of immunoglobulin absorption can be promoted, the mortality rate of the feeding livestock can be reduced, and infectious diseases can be prevented. This is an invention for new use of maltitol, and the market for maltitol can be expected to expand.

Furthermore, by feeding or orally administering Martinol to livestock during the lactation period, it is possible to improve the health of individuals up to the adult stage. For example, in dairy cows (lactating cows), milk such as improving milk production and milk quality of adult cows A favorable effect is also obtained in production.

Examples of the present invention will be described below, but the present invention is not limited to these examples, and various modifications thereof are possible within the technical idea of the present invention.

The following tests were carried out to confirm the effect on immunoglobulin absorption and serum immunoglobulin concentration of suckling livestock fed with Martinol-added artificial first milk.

The test livestock were 18 male and female Holstein cows, divided into 6 per test group, and the control group was artificial first milk (head start (registered trademark, IgG60 g / bag (225 g) included), Bayer Pharmaceutical Co., Ltd. Product) or milk replacer (Mirukuru (registered trademark), Meiji Foods Co., Ltd. product) only, the present invention zone (MAL section) is artificial first milk or milk replacer + milk replacer (Amalty MR-50 (registered trademark), Mitsubishi Shoji Food Tech Co., Ltd. product) Salary (multi-toll added amount is 18 g / time), artificial first milk or milk replacer + fructo-oligosaccharide (Meioligo-P (registered trademark), Meiji products) as comparison target group (FOS group) ) Feeding (the amount of fructo-oligosaccharide added was 18 g / dose) was performed from birth to 7 days after birth. The salaries of all three treatment areas were as follows.

1. 1. Within 1 hour of birth, feed 2 L of artificial first milk containing 120 g of IgG. 3. 10 hours after birth, feed 2 L of artificial first milk containing 120 g of IgG. 2. 24 hours after birth, give 2L of milk replacer. 3. Salary 2L of milk replacer 36 hours after birth. After 3 days of birth, 2L of milk replacer twice a day

Here, in this test, the purpose of using artificial first milk and milk replacer instead of using the first milk milked from the parent cow is to eliminate the influence of the difference in the first milk. In addition, although specific product names are given in this test, this is to indicate the identity of the specimen and does not indicate the superiority or inferiority of each product.

Then, 9 ml of blood was collected immediately before feeding within 1 hour (treated as 0 hours) to 36 hours after birth, and the same blood collection was performed on the 4th and 7th days after the 3rd day of birth, respectively. Serum IgG concentration was measured by ELISA. At the same time, the apparent IgG absorption rate (AEA: Apparent Efficiency of Absorption) was also calculated by the following formula based on the serum IgG concentration 24 hours after birth.

AEA = (body weight (kg) x 8.6% x serum IgG concentration (g / L)) / IgG feed amount x 100

Table 1 below summarizes the timing of this feeding and blood collection, and Table 2 below shows the birth weight (average) and IgG feeding amount (total amount) of calves in each ward.

The test results are shown in FIGS. 1 and 2. First, in FIG. 1, no significant difference was observed in the serum IgG concentration in each group 10 hours after birth, and 24 hours (1 day) after birth (MAL group: MAL group:). The black circle and the alternate long and short dash line) were significantly higher than those in the control group (white triangle and solid line) and the comparison target group (FOS group: black square and dotted line). The same was true after 36 hours, 4 days, and 7 days. No significant difference was observed between the control group and the comparison target group (FOS group) during the entire test period. The significance level is 5%.

In addition, as shown in FIG. 2, the apparent IgG absorption rate at the 24th hour of birth was also significantly higher in the present invention group (MAL group) than in the control group and the comparative group (FOS group). Again, no significant difference was observed between the control group and the comparison target group (FOS group). The significance level of this is also 5%.

Based on these results, it is possible to promote the absorption of IgG by feeding multitoll with IgG-containing artificial priming milk or milk replacer to suckling calves, and oligosaccharides in which 2 to 5 molecules of fructose are bound to glucose. It was confirmed that the same effect was not observed with fructose oligosaccharide (a large difference was observed in the immunopotentiating activity of suckling livestock depending on the type of sugar).

The present invention can be summarized as follows.

The present invention is a feed composition for enhancing the immunity of feeding livestock (cattle, horses, pigs, sheep, goats, etc.), which is simple, effective and highly safe, using ingredients having abundant eating experience. , The purpose is to provide a method for raising livestock during the feeding period.

Then, by feeding or orally administering a feed composition or the like containing martitol as an active ingredient to the suckling livestock, the immunity thereof is enhanced (particularly promoting the absorption of immunoglobulin), and the suckling livestock dies. It is possible to reduce the rate and prevent infectious diseases, and to carry out efficient dairy farming and livestock farming.

Claims (12)

A feed composition for enhancing immunity of suckling livestock containing maltitol as an active ingredient. The composition according to claim 1, wherein the feed composition contains first milk and / or artificial first milk. The composition according to claim 1 or 2, wherein the enhancement of immunity is promotion of immunoglobulin absorption. The composition according to claim 3, wherein the promotion of immunoglobulin absorption is the promotion of IgG absorption. The feed composition according to any one of claims 1 to 4, wherein the livestock is one or more selected from cattle, horses, pigs, sheep, and goats. A method for enhancing immunity of suckling livestock, which comprises feeding or orally administering maltitol to feeding livestock. A method for reducing mortality and / or preventing infectious diseases by enhancing immunity of suckling livestock, which comprises feeding or orally administering Martinol to feeding livestock. The method according to claim 6 or 7, wherein the enhancement of immunity is promotion of immunoglobulin absorption. The method according to claim 8, wherein the promotion of immunoglobulin absorption is the promotion of IgG absorption. The method according to any one of claims 6 to 9, wherein the method is fed or orally administered within 48 hours after birth. The method according to any one of claims 6 to 10, wherein maltitol is mixed with the first milk or the artificial first milk and fed. The method according to any one of claims 6 to 11, wherein the livestock is one or more selected from cattle, horses, pigs, sheep, and goats.

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