JP6815334B2 - 核酸の単離のための自動化可能な方法 - Google Patents
核酸の単離のための自動化可能な方法 Download PDFInfo
- Publication number
- JP6815334B2 JP6815334B2 JP2017563581A JP2017563581A JP6815334B2 JP 6815334 B2 JP6815334 B2 JP 6815334B2 JP 2017563581 A JP2017563581 A JP 2017563581A JP 2017563581 A JP2017563581 A JP 2017563581A JP 6815334 B2 JP6815334 B2 JP 6815334B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- isolation
- nucleic acids
- purification
- butanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15171261.9 | 2015-06-09 | ||
| EP15171261 | 2015-06-09 | ||
| PCT/EP2016/063150 WO2016198519A1 (en) | 2015-06-09 | 2016-06-09 | Automatable method for nucleic acid isolation |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018518171A JP2018518171A (ja) | 2018-07-12 |
| JP2018518171A5 JP2018518171A5 (enExample) | 2018-10-25 |
| JP6815334B2 true JP6815334B2 (ja) | 2021-01-20 |
Family
ID=53373331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017563581A Active JP6815334B2 (ja) | 2015-06-09 | 2016-06-09 | 核酸の単離のための自動化可能な方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10829758B2 (enExample) |
| EP (2) | EP3307907B1 (enExample) |
| JP (1) | JP6815334B2 (enExample) |
| CN (1) | CN107683335A (enExample) |
| ES (1) | ES2965460T3 (enExample) |
| WO (1) | WO2016198519A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3325697B1 (en) | 2015-07-23 | 2021-11-10 | Biocartis NV | Optimized clinical sample sequencing |
| US20200208137A1 (en) * | 2017-09-18 | 2020-07-02 | Yin To Chiu | Method for Using Aqueous Two-Phase System for the Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments |
| CA3120216A1 (en) * | 2018-11-19 | 2020-05-28 | Biocartis Nv | Enhanced detection of low-copy-number nucleic acids in an integrated workflow |
| US12565644B2 (en) * | 2019-08-08 | 2026-03-03 | Biocartis Nv | Nucleic acid purification chemistry |
| WO2021130415A1 (en) * | 2019-12-23 | 2021-07-01 | Biopsense Oy | Method, automated system and cartridge for extraction of cell-free nucleic acids from a blood sample |
| WO2024194332A1 (en) * | 2023-03-20 | 2024-09-26 | Bioecho Life Sciences Gmbh | Method for isolating nucleic acid from a biological sample |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| GB2346615B (en) * | 1998-11-17 | 2003-10-15 | Cambridge Molecular Tech | Isolating nucleic acid |
| US7183002B2 (en) * | 2000-03-24 | 2007-02-27 | Qiagen, Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| WO2004108925A1 (en) * | 2003-06-04 | 2004-12-16 | Qiagen As | Method for sequentially isolating dna and rna from the same nucleic acid-containing sample |
| JP2005137298A (ja) * | 2003-11-07 | 2005-06-02 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| CN101213022B (zh) | 2005-06-30 | 2010-06-09 | 拜欧卡蒂斯股份公司 | 自动医学诊断盒体 |
| JP2007325562A (ja) * | 2006-06-09 | 2007-12-20 | Fujifilm Corp | 核酸抽出法 |
| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| EP2128169A1 (de) | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| EP2264168B1 (de) * | 2009-06-18 | 2014-12-17 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren |
| EP2345719A1 (en) * | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Method for isolating small RNA |
| JP6096774B2 (ja) * | 2011-08-12 | 2017-03-15 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸を単離するための方法 |
| US9206469B2 (en) * | 2012-07-18 | 2015-12-08 | Zymo Research Corporation | Nucleic acid purification |
| WO2014144174A1 (en) * | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | Compositions and methods for nucleic acid extraction |
-
2016
- 2016-06-09 ES ES16732490T patent/ES2965460T3/es active Active
- 2016-06-09 JP JP2017563581A patent/JP6815334B2/ja active Active
- 2016-06-09 WO PCT/EP2016/063150 patent/WO2016198519A1/en not_active Ceased
- 2016-06-09 CN CN201680030749.8A patent/CN107683335A/zh active Pending
- 2016-06-09 US US15/570,947 patent/US10829758B2/en active Active
- 2016-06-09 EP EP16732490.4A patent/EP3307907B1/en active Active
- 2016-06-09 EP EP22216565.6A patent/EP4186980A1/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016198519A1 (en) | 2016-12-15 |
| JP2018518171A (ja) | 2018-07-12 |
| EP3307907B1 (en) | 2023-11-15 |
| EP3307907A1 (en) | 2018-04-18 |
| CN107683335A (zh) | 2018-02-09 |
| US10829758B2 (en) | 2020-11-10 |
| EP4186980A1 (en) | 2023-05-31 |
| CA2982706A1 (en) | 2016-12-15 |
| EP3307907C0 (en) | 2023-11-15 |
| US20180291367A1 (en) | 2018-10-11 |
| ES2965460T3 (es) | 2024-04-15 |
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