JP6803499B2 - Optical measurement system and optical cell - Google Patents
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Description
本発明は、光学測定システム及び光学セルに関するものである。 The present invention relates to an optical measurement system and an optical cell.
発明者らは、ＰＯＣＴ（point of care testing）に対応した光学測定装置を提案した。その一例が、特許文献１記載のＰＯＣＴ対応のＬＩＦ（Laser‐induced fluorescence）装置である。これは、光路を含む光学系をシリコーン樹脂で構成するものである。導光路の一部に照射光（励起光）及び観測光に透明な樹脂を充填し、透明な樹脂を包囲するように、迷光を吸収する特性を有する顔料を含有する樹脂を設ける。 The inventors have proposed an optical measuring device compatible with POCT (point of care testing). One example is the POCT-compatible LIF (Laser-induced fluorescence) device described in Patent Document 1. In this method, the optical system including the optical path is made of silicone resin. A part of the light guide path is filled with a transparent resin for irradiation light (excitation light) and observation light, and a resin containing a pigment having a property of absorbing stray light is provided so as to surround the transparent resin.
上記透明な樹脂と、顔料含有樹脂との材質を同じにすることにより、以下のような利点が得られる。まず、両樹脂の界面での反射・散乱が抑制される。次に、顔料含有樹脂に入射した迷光が当該樹脂で吸収され導光路に殆ど戻らず、迷光の複雑な多重反射がほとんど発生しない。さらに、外部からの外光も導光路に到達しない。 By using the same material for the transparent resin and the pigment-containing resin, the following advantages can be obtained. First, reflection / scattering at the interface between the two resins is suppressed. Next, the stray light incident on the pigment-containing resin is absorbed by the resin and hardly returns to the light guide path, and complicated multiple reflection of the stray light hardly occurs. Further, the external light from the outside does not reach the light guide path.
よって、光学測定装置の光学系は、複雑な多重反射に対応する必要がない。よって、光学系は小型・簡便化される。結果として、光学測定装置も小型化される。上記したシリコーン樹脂で構築した光学系の技術を、ＳＯＴ（Silicone Optical Technologies）と呼称することにする。 Therefore, the optical system of the optical measuring device does not need to cope with complicated multiple reflections. Therefore, the optical system is made smaller and simpler. As a result, the optical measuring device is also miniaturized. The optical system technology constructed of the above-mentioned silicone resin will be referred to as SOT (Silicone Optical Technologies).
ＳＯＴ技術を用いた光学系は、上記したようなＬＩＦ装置以外の光学測定装置に採用されうる。例えば、図１２に示すような吸光度計１０１にも採用可能である。 An optical system using SOT technology can be adopted in an optical measuring device other than the LIF device as described above. For example, it can also be used for the absorbance meter 101 as shown in FIG.
図１２（ａ）の吸光度計１０１は、遮光性の筐体１０３（顔料含有樹脂からなる筐体）内に、検体１０５を内包するＰＣＲ管１０７が設置される導光路１０９と、導光路１０９の一端に設定されるＬＥＤ等の光源１１１と、導光路１０９の他端に設置される受光センサ１１３からなる。導光路１０９は、光源１１１から放出されＰＣＲ管１０７に内包されている検体１０５に照射される照射光１１５と、照射光１１５が照射された検体１０５から放出される観測光１１７に対して透明な樹脂が充填された透明樹脂製導光路である。 The absorbance meter 101 of FIG. 12A shows a light guide path 109 in which a PCR tube 107 containing a sample 105 is installed in a light-shielding housing 103 (a housing made of a pigment-containing resin), and a light guide path 109. It includes a light source 111 such as an LED set at one end and a light receiving sensor 113 installed at the other end of the light guide path 109. The light guide path 109 is transparent to the irradiation light 115 emitted from the light source 111 and irradiated to the sample 105 contained in the PCR tube 107 and the observation light 117 emitted from the sample 105 irradiated with the irradiation light 115. It is a transparent resin light guide path filled with resin.
導光路１０９が透明樹脂製である図１２（ａ）と比して、図１２（ｂ）の吸光度計１１９における導光路１２１は空洞である。図１２（ｂ）のように、導光路１２１に透明樹脂を充填せず、空洞のままにした場合は、導光路１２１と筐体１０３（顔料含有樹脂）との界面における迷光反射の抑制効果は得られないものの、顔料含有樹脂に入射した迷光が当該樹脂で吸収され導光路１２１に殆ど戻らず、迷光の複雑な多重反射がある程度「抑制」される。外部からの外光も導光路１２１に到達しないという効果は得られる。 Compared with FIG. 12 (a) in which the light guide path 109 is made of a transparent resin, the light guide path 121 in the absorbance meter 119 in FIG. 12 (b) is hollow. As shown in FIG. 12B, when the light guide path 121 is not filled with the transparent resin and is left hollow, the effect of suppressing stray light reflection at the interface between the light guide path 121 and the housing 103 (pigment-containing resin) is obtained. Although not obtained, the stray light incident on the pigment-containing resin is absorbed by the resin and hardly returns to the light guide path 121, and the complicated multiple reflection of the stray light is "suppressed" to some extent. The effect that the external light from the outside does not reach the light guide path 121 can be obtained.
このようなＰＯＣＴに対応したポータブルの吸光度計としては、ウシオ電機製Picoscope（登録商標）などがある。 As a portable absorbance meter compatible with such POCT, there is Picoscope (registered trademark) manufactured by Ushio, Inc.
上記のＳＯＴ技術を採用した光学測定装置では、光路内に測定試料を内部に保持したままの汎用チューブ（ＰＣＲ管）を設置することで、試料の測定を実施可能とすることができる。よって、大型の光学測定装置を用いる場合に発生する、試料を測定セルに打移し替えるためのピペットなどを用いる作業が不要となる。 In the optical measuring device adopting the above-mentioned SOT technology, it is possible to measure the sample by installing a general-purpose tube (PCR tube) in which the measurement sample is held inside in the optical path. Therefore, the work of using a pipette or the like for transferring the sample to the measurement cell, which occurs when a large-scale optical measuring device is used, becomes unnecessary.
しかしながら、一般にＰＣＲ管はポリプロピレンで構成される。ポリプロピレンは、照射される光の波長４００ｎｍより短くなるにつれ、光吸収のため光透過率が減少する。そのため、一般のＰＣＲ管を用いる場合、波長３００ｎｍ以下の光を用いた光学測定は、実質、不可能となる。 However, PCR tubes are generally made of polypropylene. The light transmittance of polypropylene decreases due to light absorption as the wavelength of the irradiated light becomes shorter than 400 nm. Therefore, when a general PCR tube is used, optical measurement using light having a wavelength of 300 nm or less becomes practically impossible.
ライフサイエンス分野において、紫外線を用いた吸光度測定の要請は大きい。例えば、ＤＮＡを構成する４種類の各塩基（アデニン、グアニン、シトシン、チミン）の最大吸収波長は、２５０〜２７０ｎｍの波長域内にある。よって、この波長域の光（紫外線）を用いた吸光度測定を行うことにより、ＤＮＡの定量を実施することが可能となる。また、タンパク質は、波長２８０ｎｍ付近の紫外線をよく吸収する。これは、トリプトファン・チロシン・フェニルアラニンの芳香族のベンゼン環がこの付近に吸収ピークをもつことに由来する。そのため、波長２８０ｎｍの光を用いた吸光度測定を行うことにより、タンパク質の定量を実施することが可能となる。 In the field of life science, there is a great demand for absorbance measurement using ultraviolet rays. For example, the maximum absorption wavelength of each of the four types of bases (adenine, guanine, cytosine, thymine) constituting DNA is in the wavelength range of 250 to 270 nm. Therefore, it is possible to quantify DNA by measuring the absorbance using light (ultraviolet rays) in this wavelength range. In addition, the protein absorbs ultraviolet rays having a wavelength of around 280 nm well. This is because the aromatic benzene ring of tryptophan, tyrosine, and phenylalanine has an absorption peak in the vicinity. Therefore, it is possible to quantify proteins by measuring the absorbance using light having a wavelength of 280 nm.
紫外線を用いた光学測定用のサンプルケースは、例えば、紫外線透過性が良好な石英ガラスから構成される。但し、石英ガラスからなるサンプルケースは比較的高価であり、また衝撃に対して脆い。よって、ＰＯＣＴ用の光学測定装置に使用されるサンプルケースとしては、測定現場でのハンドリング性がよくない。 The sample case for optical measurement using ultraviolet rays is made of, for example, quartz glass having good ultraviolet transmission. However, the sample case made of quartz glass is relatively expensive and brittle to impact. Therefore, as a sample case used in an optical measuring device for POCT, the handleability at the measurement site is not good.
また、特許文献２には、紫外線透過性のサンプルケースとして、紫外線透過性プラスチックからなるキュベットが開示されている。このキュベットは、石英ガラスからなるサンプルケースと比較すると、耐衝撃性が良好である。しかしながら、このサンプルケースは、特定の光学測定装置に対応したものである。他の光学測定装置に対応させるには、アダプターが必要となる。 Further, Patent Document 2 discloses a cuvette made of an ultraviolet-transmissive plastic as an ultraviolet-transmissive sample case. This cuvette has better impact resistance than a sample case made of quartz glass. However, this sample case is compatible with a particular optical measuring device. An adapter is required to support other optical measuring devices.
さらに、特許文献３、４には、体積マイクロリットルオーダーの測定試料（液体）を、表面張力を利用して円筒状に保持し、当該試料を光学測定する方法・装置が開示されている。このような装置を用いることにより、試料をサンプルケースに保持することなく、紫外線を用いた光学測定を行うことが可能となる。しかしながら、マイクロリットルオーダーの測定試料は、蒸発しやすく、光学測定中に試料を通過する通過光の光路が絶えず変化し、安定した光学測定が非常に困難となる。 Further, Patent Documents 3 and 4 disclose a method / apparatus for holding a measurement sample (liquid) on the order of microliters in volume in a cylindrical shape by utilizing surface tension and optically measuring the sample. By using such an apparatus, it is possible to perform optical measurement using ultraviolet rays without holding the sample in the sample case. However, a measurement sample on the order of microliters tends to evaporate, and the optical path of the passing light passing through the sample changes constantly during the optical measurement, which makes stable optical measurement very difficult.
このように従来技術では、測定現場において、ＤＮＡやタンパク質を高精度で測定することは出来なかった。 As described above, in the prior art, it was not possible to measure DNA and protein with high accuracy at the measurement site.
そこで、本発明は、紫外線領域も測定可能で、測定現場でも取扱やすくＰＯＣＴに適した光学システム及び光学セルを新たに提供することを目的とする。 Therefore, it is an object of the present invention to newly provide an optical system and an optical cell that can measure an ultraviolet region and are easy to handle even at a measurement site and are suitable for POCT.
本発明の第１の観点は、試料の光学測定を行う光学測定装置と光学セルからなる光学測定システムであって、前記光学測定装置は、前記試料に入射光を入射させる光源部と、前記試料からの出射光を測定する光検出部と、前記光源部から前記光学セルへの前記入射光を導光する第１導光路部と、前記光学セルから前記光検出部への前記出射光を導光する第２導光路部とを有し、前記光学セルは、光学測定対象の試料を保持するための中空部と、前記中空部を取り囲む周壁部の一部に、前記入射光が透過する入射部と、前記出射光が透過する出射部とを有し、前記入射部と前記出射部が、紫外線透過性樹脂からなる、光学測定システムである。 The first aspect of the present invention is an optical measurement system including an optical measurement device for performing optical measurement of a sample and an optical cell, wherein the optical measurement device includes a light source unit for incident light incident on the sample and the sample. A light detection unit that measures the emitted light from the optical cell, a first light guide path unit that guides the incident light from the light source unit to the optical cell, and the emitted light from the optical cell to the optical detection unit. The optical cell has a second light guide path portion that shines, and the optical cell is an incident light transmitted through a hollow portion for holding a sample to be optically measured and a part of a peripheral wall portion surrounding the hollow portion. It is an optical measurement system having a unit and an emitting unit through which the emitted light is transmitted, and the incident portion and the emitting portion are made of an ultraviolet transmissive resin.
本発明の第２の観点は、第１の観点の光学測定システムであって、前記第１導光路部と前記入射部は、それぞれの一部が接する接触面を有し、屈折率が同一の紫外線透過性樹脂からなり、前記出射部と前記第２導光路部は、それぞれの一部が接する接触面を有し、屈折率が同一の紫外線透過性樹脂からなる、光学測定システムである。 The second aspect of the present invention is the optical measurement system of the first aspect, in which the first light guide path portion and the incident portion each have a contact surface in contact with each other and have the same refractive index. It is an optical measurement system made of an ultraviolet transmissive resin, and the emitting portion and the second light guide path portion each have a contact surface in contact with each other and are made of an ultraviolet transmissive resin having the same refractive index.
本発明の第３の観点は、光学測定対象の試料を保持するための中空部を有する光学セルであって、前記中空部を取り囲む周壁部の一部に、外部光源から前記中空部に入射させる入射光が透過する入射部と、前記中空部から外部光検出器へ出射させる出射光が透過する出射部とを備え、前記入射部と前記出射部が、紫外線透過性樹脂からなる、光学セルである。 A third aspect of the present invention is an optical cell having a hollow portion for holding a sample to be optically measured, and a part of a peripheral wall portion surrounding the hollow portion is incident on the hollow portion from an external light source. An optical cell comprising an incident portion through which incident light is transmitted and an exit portion through which emission light emitted from the hollow portion to an external light detector is transmitted, and the incident portion and the exit portion are made of an ultraviolet transmissive resin. is there.
本発明の第４の観点は、第３の観点の光学セルであって、前記入射部又は出射部が、特定の波長又は波長域の光を吸収する色素を含有する色素含有紫外線透過性樹脂からなる、光学セルである。 A fourth aspect of the present invention is the optical cell of the third aspect, from a dye-containing ultraviolet transmissive resin in which the incident portion or the emitting portion contains a dye that absorbs light in a specific wavelength or wavelength range. It is an optical cell.
本発明の第５の観点は、第４の観点の光学セルであって、前記色素含有紫外線透過性樹脂が色素拡散防止部材で囲まれている、光学セルである。 The fifth aspect of the present invention is the optical cell of the fourth aspect, in which the dye-containing ultraviolet transmissive resin is surrounded by a dye diffusion prevention member.
本発明の第６の観点は、第３から５のいずれかの観点の光学セルであって、前記入射部と前記出射部は、対向する面の少なくとも一部が平行である、光学セルである。 A sixth aspect of the present invention is an optical cell according to any one of the third to fifth aspects, wherein at least a part of the opposing surfaces is parallel to the incident portion and the exit portion. ..
本発明の第７の観点は、第３から６のいずれかの観点の光学セルであって、前記中空部は、外部に開放された開口部と、前記入射部及び前記出射部に隣接する試料溜まり部と、前記開口部から前記試料溜まり部までをつなぐ流路を有し、少なくとも前記開口部及び前記試料溜まり部が、中心軸の周りにｎ回（ｎは自然数）軸対称の形状であり、前記中心軸に沿って一端に前記開口部を備えると共に他端に前記試料溜まり部を備えるものであり、前記試料溜まり部の一部における前記中心軸に垂直な断面積が、前記流路の一部における前記中心軸に垂直な断面積よりも大きい、光学セルである。 A seventh aspect of the present invention is an optical cell according to any one of the third to sixth aspects, wherein the hollow portion has an opening opened to the outside and a sample adjacent to the incident portion and the exit portion. It has a reservoir and a flow path connecting the opening to the sample reservoir, and at least the opening and the sample reservoir have an axisymmetric shape n times (n is a natural number) around the central axis. Along the central axis, the opening is provided at one end and the sample reservoir is provided at the other end, and the cross-sectional area of a part of the sample reservoir perpendicular to the central axis is the flow path of the flow path. An optical cell that is larger than the cross-sectional area perpendicular to the central axis in some parts.
本発明の第８の観点は、第７の観点の光学セルであって、前記開口部が漏斗状になっており、前記開口部の一部における前記中心軸に垂直な断面積が、前記流路の一部における前記中心軸に垂直な断面積よりも大きい、光学セルである。 The eighth aspect of the present invention is the optical cell of the seventh aspect, in which the opening is funnel-shaped, and the cross-sectional area of a part of the opening perpendicular to the central axis is the flow. An optical cell that is larger than the cross-sectional area perpendicular to the central axis in a part of the path.
本発明の第９の観点は、第７又は８の観点の光学セルであって、前記試料溜まり部の容積が１０μｌ以下であり、前記流路における前記中心軸に垂直な断面積が１ｍｍ２以下である、光学セルである。 The ninth aspect of the present invention is the optical cell according to the seventh or eighth aspect, in which the volume of the sample reservoir is 10 μl or less and the cross-sectional area perpendicular to the central axis in the flow path is 1 mm 2 or less. Is an optical cell.
本発明の第１０の観点は、第３から９のいずれかの観点の光学セルであって、前記中空部から外部へつながる空気孔部をさらに備える、光学セルである。 A tenth aspect of the present invention is an optical cell according to any one of the third to ninth aspects, further including an air hole portion connecting the hollow portion to the outside.
本発明の第１１の観点は、第３から１０のいずれかの観点の光学セルであって、前記紫外線透過性樹脂が、ジメチルポリシロキサン（ＰＤＭＳ）である、光学セルである。 The eleventh aspect of the present invention is an optical cell according to any one of the third to ten aspects, wherein the ultraviolet transmissive resin is dimethylpolysiloxane (PDMS).
本発明の第１２の観点は、第３から６のいずれかの観点の光学セルであって、前記中空部は、試料を入れる試料導入口と、試料を出す試料導出口を有し、前記入射部及び前記出射部を除く前記周壁部は、迷光を吸収する色素を含有する迷光吸収色素含有樹脂からなる、光学セルである。 The twelfth aspect of the present invention is an optical cell according to any one of the third to sixth aspects, wherein the hollow portion has a sample introduction port for inserting a sample and a sample outlet for discharging a sample, and the incident The peripheral wall portion excluding the portion and the emission portion is an optical cell made of a stray light absorbing dye-containing resin containing a dye that absorbs stray light.
本発明の各観点によれば、屋外等の測定現場でも取扱いやすく、入射光が紫外線領域でも測定可能な光学測定システム等を提供することが可能になる。 According to each viewpoint of the present invention, it is possible to provide an optical measurement system or the like that is easy to handle even at a measurement site such as outdoors and can measure incident light even in an ultraviolet region.
本願発明の第２の観点によれば、第１導光路及び入射部、並びに、第２導光路及び出射部は、それぞれ屈折率が同一の紫外線透過性樹脂からなるため、両樹脂が接触する接触面での反射・散乱が抑制される。 According to the second aspect of the present invention, the first light guide path and the incident portion, and the second light guide path and the exit portion are each made of an ultraviolet transmissive resin having the same refractive index, so that the two resins come into contact with each other. Reflection / scattering on the surface is suppressed.
本発明の第３の観点によれば、屋外等の測定現場でも取扱いやすく、入射光が紫外線領域でも測定可能な光学セルを提供することが可能になる。 According to the third aspect of the present invention, it is possible to provide an optical cell that is easy to handle even at a measurement site such as outdoors and can measure incident light even in an ultraviolet region.
本発明の第４の観点によれば、光学フィルタとしても機能する光学セルを提供することが可能となる。さらに、副次的な効果として、試料や観測光等に応じて、性能の異なる光学フィルタに変更することが容易になる。ＳＯＴ技術を用いた光学測定装置では、導光路は樹脂で充填され、さらに周囲を顔料含有樹脂で覆われているため、光学素子を固定ではなく取り替え可能にするためには複雑な構造を要する。通常の光学測定装置で出し入れ可能な構造になっている光学セルに光学フィルタの機能を併せ持たせることで、光学測定装置の構造を変えることなく、光学フィルタの変更が可能になる。 According to the fourth aspect of the present invention, it is possible to provide an optical cell that also functions as an optical filter. Further, as a secondary effect, it becomes easy to change to an optical filter having different performance depending on the sample, the observed light, and the like. In the optical measuring device using the SOT technology, since the light guide path is filled with a resin and the periphery is covered with a pigment-containing resin, a complicated structure is required to make the optical element replaceable instead of fixed. By adding the function of the optical filter to the optical cell having a structure that can be taken in and out of a normal optical measuring device, the optical filter can be changed without changing the structure of the optical measuring device.
本発明の第５の観点によれば、色素含有紫外線透過性樹脂から、隣接する第１導光路や第２導光路への色素の拡散を防止することができる。 According to the fifth aspect of the present invention, it is possible to prevent the dye from diffusing from the dye-containing ultraviolet transmissive resin into the adjacent first light guide path and the second light guide path.
本発明の第６の観点によれば、光学セルの設置による測定誤差を小さくすることが容易となる。 According to the sixth aspect of the present invention, it becomes easy to reduce the measurement error due to the installation of the optical cell.
本発明の第７の観点によれば、貴重な試料の必要最小限の量で光学測定を行うことが容易になる。また、試料溜まり部では十分な光路長を確保しつつ、試料が空気に接する面積を抑えることが容易になる。 According to the seventh aspect of the present invention, it becomes easy to perform optical measurement with the minimum necessary amount of a valuable sample. In addition, it becomes easy to suppress the area where the sample is in contact with air while ensuring a sufficient optical path length in the sample pool portion.
本発明の第８の観点によれば、開口部が漏斗状になっているため、試料の注入が容易となる。 According to the eighth aspect of the present invention, the funnel-shaped opening facilitates injection of the sample.
本発明の第９の観点によれば、少量の試料でも蒸発を抑制して安定した測定を可能としつつ、試料の流入がスムーズな光学セルを提供することが容易となる。 According to the ninth aspect of the present invention, it becomes easy to provide an optical cell in which the inflow of the sample is smooth while suppressing evaporation even in a small amount of sample and enabling stable measurement.
本発明の第１０の観点によれば、中空部の容積が小さくても試料を試料溜まり部に流入させることが容易となる。 According to the tenth aspect of the present invention, even if the volume of the hollow portion is small, it is easy to allow the sample to flow into the sample reservoir.
本発明の第１１の観点によれば、ＤＮＡやタンパク質などの生体関連物質の測定に適した生体親和性の高い光学セルを提供することが可能となる。 According to the eleventh aspect of the present invention, it is possible to provide an optical cell having high biocompatibility suitable for measuring a biorelated substance such as DNA or protein.
本発明の第１２の観点によれば、屋外等の測定現場でも取扱いやすく、入射光が紫外線領域でも測定可能なフローセルを提供することが可能になる。 According to the twelfth aspect of the present invention, it is possible to provide a flow cell that is easy to handle even at a measurement site such as outdoors and can measure incident light even in an ultraviolet region.
図１は、本発明のサンプルケース１（本願請求項記載の「光学セル」の一例）と吸光度計３（本願請求項記載の「光学測定装置」の一例）からなる光学測定システム５（本願請求項記載の「光学測定システム」の一例）の断面図である。 FIG. 1 shows an optical measurement system 5 (claimed by the present application) comprising a sample case 1 of the present invention (an example of the “optical cell” described in the present invention) and an absorbance meter 3 (an example of the “optical measuring device” described in the present claim). It is sectional drawing of an example of "optical measurement system" described in the item.
サンプルケース１は、紫外線透過特性を有する汎用の樹脂（エラストマー）（本願請求項記載の「紫外線透過性樹脂」の一例）を用いて構成した。樹脂には、例えば、ジメチルポリシロキサン（ＰＤＭＳ）を用いる。サンプルケース１が紫外線透過性樹脂であるため、紫外線（例えば、２６０ｎｍ、２８０ｎｍ）で試料（ＤＮＡ、タンパク質）の定量を行うことができる。また、ＰＤＭＳは生体親和性が高いため、ＤＮＡやタンパク質等の生体試料のサンプルケースに適している。 The sample case 1 was constructed by using a general-purpose resin (elastomer) having an ultraviolet transmissive property (an example of the “ultraviolet transmissive resin” described in the present claims). As the resin, for example, dimethylpolysiloxane (PDMS) is used. Since the sample case 1 is an ultraviolet transmissive resin, the sample (DNA, protein) can be quantified with ultraviolet rays (for example, 260 nm and 280 nm). Moreover, since PDMS has high biocompatibility, it is suitable as a sample case for biological samples such as DNA and proteins.
さらに、エラストマーを用いてサンプルケース１を構成したので、製造上の自由度が高く、容易に所望の形に成形できる。そのため、光学測定装置に応じて、それにフィットする形状のサンプルケースを形成できる。また、弾性特性があるので、耐衝撃性が良好である。よって、ＰＯＣＴにおける測定現場でのハンドリングが容易である。 Further, since the sample case 1 is constructed by using an elastomer, it has a high degree of freedom in manufacturing and can be easily formed into a desired shape. Therefore, depending on the optical measuring device, a sample case having a shape that fits the sample case can be formed. Moreover, since it has elastic properties, it has good impact resistance. Therefore, it is easy to handle at the measurement site in POCT.
しかも、大量生産により、製造コストを安価にすることができる見込みがある。 Moreover, mass production is expected to reduce manufacturing costs.
吸光度計３は、光源７（本願請求項記載の「光源部」の一例）と、光検出器９（本願請求項記載の「光検出部」の一例）と、光源７から試料１１へ入射光１３を導光する第１透明樹脂製導光路１５（本願請求項記載の「第１導光路部」の一例）と、試料１１から光検出器９へ出射光１７を導光する第２透明樹脂製導光路１９（本願請求項記載の「第２導光路部」の一例）と、迷光を吸収する特性を有する顔料を含有する顔料含有樹脂製筐体２１で構成される。さらに、第１透明樹脂製導光路１５、第２透明樹脂製導光路１９及びサンプルケース１（本願請求項記載の「光学セル」の一例）は、同一の材料、例えばＰＤＭＳからなるため、接触面（本願請求項記載の「接触面」の一例）において反射・散乱が抑制され、高精度な光学測定が実施できる。 The absorbance meter 3 includes a light source 7 (an example of the “light source unit” described in the present application claim), a photodetector 9 (an example of the “light detection unit” described in the present application claim), and incident light from the light source 7 to the sample 11. A first transparent resin light guide path 15 that guides the light 13 (an example of the "first light guide path portion" described in the present application) and a second transparent resin that guides the emitted light 17 from the sample 11 to the photodetector 9. It is composed of a light guide path 19 (an example of the "second light guide path portion" described in the claim of the present application) and a pigment-containing resin housing 21 containing a pigment having a property of absorbing stray light. Further, since the first transparent resin light guide path 15, the second transparent resin light guide path 19, and the sample case 1 (an example of the "optical cell" described in the present application claim) are made of the same material, for example, PDMS, they have contact surfaces. Reflection / scattering is suppressed in (an example of the "contact surface" described in the claim of the present application), and highly accurate optical measurement can be performed.
図２は、本発明のサンプルケース３１（本願請求項記載の「光学セル」の一例）の図であり、（ａ）は平面図、（ｂ）はＡ−Ａ断面図、（ｃ）はＢ−Ｂ断面図、（ｄ）はＣ方向から見たときの側面図である。 2A and 2B are views of the sample case 31 of the present invention (an example of the “optical cell” described in the claims of the present application), (a) is a plan view, (b) is a sectional view taken along the line AA, and FIG. 2C is a B. -B sectional view, (d) is a side view when viewed from the C direction.
例えば、ＤＮＡ、タンパク質の定量を行う場合、試料（ＤＮＡやタンパク質）の量は、数μｌしかない。よって、図２に示すように、ＵＶ透過性樹脂３３からなる本体に、数μｌ分の試料溜まり部３５（本願請求項記載の「試料溜まり部」の一例）を設けてもよい。特に、測定試料として１０μｌ以下の液体試料を対象とし、試料溜まり部３５の水平方向（図２（ｂ）における横方向）の断面積よりも、試料が通る流路３７（本願請求項記載の「流路」の一例）の水平方向の断面積を小さくすることで、当該液体試料が空気と接する面積が１ｍｍ２以下となるように構成した。このように構成することで、１０μｌ以下という微量な測定試料の蒸発が抑制され、安定した光学測定が可能となる。
試料注入部３９（本願請求項記載の「開口部」の一例）は、漏斗状であってもよい。数μｌの試料の注入だと、試料溜まり部３５などの空気の影響で当該試料溜まり部３５に試料が注入できない場合もある。それに対応するために、空気抜き用孔部４１（本願請求項記載の「空気孔部」の一例）を設けてもよい。
For example, when quantifying DNA and protein, the amount of sample (DNA and protein) is only a few μl. Therefore, as shown in FIG. 2, a sample reservoir 35 for several μl (an example of the “sample reservoir” described in the claims of the present application) may be provided in the main body made of the UV transmissive resin 33. In particular, a liquid sample of 10 μl or less is targeted as a measurement sample, and the flow path 37 through which the sample passes is more than the cross-sectional area of the sample reservoir 35 in the horizontal direction (horizontal direction in FIG. By reducing the cross-sectional area of the "flow path" in the horizontal direction, the area of the liquid sample in contact with air is 1 mm 2 or less. With this configuration, evaporation of a small amount of measurement sample of 10 μl or less is suppressed, and stable optical measurement becomes possible.
The sample injection unit 39 (an example of the “opening” described in the claims of the present application) may have a funnel shape. When a few μl of a sample is injected, the sample may not be injected into the sample reservoir 35 due to the influence of air such as the sample reservoir 35. In order to cope with this, an air vent hole 41 (an example of the “air hole” described in the claims of the present application) may be provided.
また、図２に示すように、流路３７、試料溜まり部３５は、中心軸e（本願請求項記載の「中心軸」の一例）周りにｎ回（ｎは自然数）軸対称の形状に構成してもよい。 Further, as shown in FIG. 2, the flow path 37 and the sample collecting portion 35 are configured to have an axisymmetric shape n times (n is a natural number) around the central axis e (an example of the “central axis” described in the claims of the present application). You may.
本実施例において、試料注入部３９、流路３７、試料溜まり部３５により、サンプルケース３１の中空部（本願請求項記載の「中空部」の一例）が形成される。すなわち、この中空部はＵＶ透過性樹脂３３（本願請求項記載の「周壁部」の一例）により取り囲まれる。 In this embodiment, the sample injection portion 39, the flow path 37, and the sample pool portion 35 form a hollow portion of the sample case 31 (an example of the “hollow portion” described in the claims of the present application). That is, this hollow portion is surrounded by the UV transmissive resin 33 (an example of the "peripheral wall portion" described in the claims of the present application).
図３は、図２のサンプルケース３１と吸光度計３からなる光学測定システム４３（本願請求項記載の「光学測定システム」の一例）の断面図である。吸光度計３は、図１の実施例１と同様の構成である。 FIG. 3 is a cross-sectional view of an optical measurement system 43 (an example of the “optical measurement system” described in the present invention) including the sample case 31 of FIG. 2 and the absorbance meter 3. The absorbance meter 3 has the same configuration as that of Example 1 of FIG.
図３においては、図２に示すサンプルケース３１の試料溜まり部３５に光源７からの入射光１３が導光される位置に、第１透明樹脂製導光路１５が設けられている。この配置において、図２に示すサンプルケース３１のＵＶ透過性樹脂３３の一方の面が第１透明樹脂製導光路１５の光出射端と接触し、他方の面が試料１１と接触している部分が入射部１４（本願請求項記載の「入射部」の一例）となる。 In FIG. 3, a first transparent resin light guide path 15 is provided at a position where the incident light 13 from the light source 7 is guided to the sample collecting portion 35 of the sample case 31 shown in FIG. In this arrangement, a portion where one surface of the UV transmissive resin 33 of the sample case 31 shown in FIG. 2 is in contact with the light emitting end of the first transparent resin light guide path 15 and the other surface is in contact with the sample 11. Is the incident portion 14 (an example of the “incident portion” described in the claims of the present application).
また、図３においては、図２に示すサンプルケース３１の試料溜まり部３５が保持する試料１１からの出射光１９が光検出器９に導光されている位置に、第２透明樹脂製導光路１９が設けられている。この配置において、図２に示すサンプルケース３１のＵＶ透過性樹脂３３の一方の面が第２透明樹脂製導光路１９の光入射端と接触し、他方の面が試料１１と接触している部分が出射部１６（本願請求項記載の「出射部」の一例）となる。 Further, in FIG. 3, a second transparent resin light guide path is located at a position where the light emitted from the sample 11 held by the sample pool 35 of the sample case 31 shown in FIG. 2 is guided to the photodetector 9. 19 is provided. In this arrangement, a portion where one surface of the UV transmissive resin 33 of the sample case 31 shown in FIG. 2 is in contact with the light incident end of the second transparent resin light guide path 19 and the other surface is in contact with the sample 11. Is the exit unit 16 (an example of the “emission unit” described in the claims of the present application).
図２に戻り、試料溜まり部３５の形状は任意である。しかしながら、図３に示すように、試料溜まり部３５の一部が入射部１４、出射部１６を構成する場合は、互いに対向する入射部１４の試料１１と接触する面と出射部１６の試料１１と接触する面の少なくとも一部は平行であることが好ましい。このような構成を取ることにより、光源７と光検出器９とのアライメントを精密に行わずとも、高精度の光学測定が可能となる。
図２（ｂ）（ｃ）に示すように、試料溜まり部３５は直方体形状に構成してもよい。このように構成することにより、２組の互いに対向する面はそれぞれ平行となる。
また、図３に示すように、この直方体形状の試料溜まり部３５が保持する試料１１に光照射して光学測定を行う場合は、図２（ｂ）に示す直方体の長手方向（面間距離f）に光が通過するように構成することが好ましい。このように構成することにより、図２（ｃ）に示す直方体の短手方向（面間距離g）に光が通過する場合と比べて、試料１１を通過する光の距離（光路長）が長くなるので、高精度の光学測定が可能となる。
Returning to FIG. 2, the shape of the sample collecting portion 35 is arbitrary. However, as shown in FIG. 3, when a part of the sample collecting portion 35 constitutes the incident portion 14 and the exit portion 16, the surface of the incident portion 14 facing each other and the sample 11 of the exit portion 16 are in contact with each other. It is preferable that at least a part of the surface in contact with is parallel. By adopting such a configuration, high-precision optical measurement becomes possible without precisely aligning the light source 7 and the photodetector 9.
As shown in FIGS. 2 (b) and 2 (c), the sample reservoir 35 may be formed in a rectangular parallelepiped shape. With this configuration, the two sets of faces facing each other are parallel to each other.
Further, as shown in FIG. 3, when the sample 11 held by the rectangular parallelepiped-shaped sample reservoir 35 is irradiated with light for optical measurement, the rectangular parallelepiped shown in FIG. 2B is shown in the longitudinal direction (inter-plane distance f). ) Is preferably configured to allow light to pass through. With this configuration, the distance (optical path length) of light passing through the sample 11 is longer than in the case where light passes in the lateral direction (inter-plane distance g) of the rectangular body shown in FIG. 2 (c). Therefore, high-precision optical measurement becomes possible.
図３のように、光学系がＳＯＴ構造の光学系を有する吸光度計３に、図２のサンプルケース３１を配置することにより、迷光が少なく小型の光学測定システム４３を構成できるため、臨床現場において、少量のＤＮＡやタンパク質等の試料１１を採取してすぐに測定を行うことが可能である。 As shown in FIG. 3, by arranging the sample case 31 of FIG. 2 on the absorbance meter 3 having an optical system having an SOT structure, a compact optical measurement system 43 with less stray light can be configured. It is possible to collect a small amount of sample 11 such as DNA or protein and immediately perform the measurement.
図４は、本発明のサンプルケース５１（本願請求項記載の「光学セル」の一例）の図であり、（ａ）は平面図、（ｂ）はＡ−Ａ断面図、（ｃ）はＢ方向から見たときの側面図である。図５は、図４のサンプルケース５１と吸光度計５３からなる光学測定システム５５（本願請求項記載の「光学測定システム」の一例）の断面図である。サンプルケース５１は、ＵＶ透過性樹脂５７からなるので、任意の形に成形することができるため、図４に示すように、外形を汎用のＰＣＲ管形状に構成することが可能である。サンプルケース５１は、ＵＶ透過性樹脂５７の外形以外の３５〜４１の構成は、実施例２の図２と同様である。 4A and 4B are views of the sample case 51 of the present invention (an example of the “optical cell” described in the claims of the present application), (a) is a plan view, (b) is a sectional view taken along the line AA, and FIG. It is a side view when viewed from a direction. FIG. 5 is a cross-sectional view of an optical measurement system 55 (an example of the “optical measurement system” according to the present claim) including the sample case 51 and the absorbance meter 53 of FIG. Since the sample case 51 is made of the UV transmissive resin 57, it can be molded into any shape, so that the outer shape can be formed into a general-purpose PCR tube shape as shown in FIG. The configuration of the sample case 51 other than the outer shape of the UV transmissive resin 57 is the same as that of FIG. 2 of the second embodiment.
これにより、図５に示すように、サンプルケースとしてＰＣＲ管を用いる吸光度計５３等の光学測定装置に対して、装置自体のサンプル保持構造を変更することなく、本発明のサンプルケース５１を設置することができる。吸光度計５３は、サンプル保持構造以外の７〜２１の構成は、実施例２の図３と同様である。 As a result, as shown in FIG. 5, the sample case 51 of the present invention is installed in an optical measuring device such as an absorbance meter 53 using a PCR tube as a sample case without changing the sample holding structure of the device itself. be able to. The configurations of the absorbance meters 53 other than the sample holding structure of 7 to 21 are the same as those of FIG. 3 of Example 2.
図６は、本発明のサンプルケース（本願請求項記載の「光学セル」の一例）の断面図であり、（ａ）はキュベット形状のサンプルケース６１、（ｂ）はＰＣＲ管形状のサンプルケース６３である。図７は、図６（ａ）のキュベット形状のサンプルケース６１と吸光度計３からなる光学測定システム６５（本願請求項記載の「光学測定システム」の一例）の断面図である。サンプルケースはＵＶ透過性樹脂からなるので、成形時に色素（染料）を分散させることが可能である。図６において、３５〜４１の構成は図２又は図４と同様であるが、ＵＶ透過性樹脂３３・５７を、ＵＶを透過してＵＶ以外の光の少なくとも一部を吸収する色素を分散させた色素分散ＵＶ透過性樹脂６７・６９（本願請求項記載の「色素含有紫外線透過性樹脂」の一例）にすることにより、図６のサンプルケース６３は一種の光学フィルタとして機能する。つまり、サンプルケースに光学的なフィルタ性能を付与することができる。 6A and 6B are cross-sectional views of a sample case of the present invention (an example of an “optical cell” described in the present claim), where (a) is a cuvette-shaped sample case 61 and (b) is a PCR tube-shaped sample case 63. Is. FIG. 7 is a cross-sectional view of an optical measurement system 65 (an example of the “optical measurement system” described in the claims of the present application) including the cuvette-shaped sample case 61 and the absorbance meter 3 of FIG. 6 (a). Since the sample case is made of a UV-transparent resin, it is possible to disperse the dye during molding. In FIG. 6, the configuration of 35 to 41 is the same as that of FIG. 2 or 4, but the UV-transparent resins 33 and 57 are dispersed with a dye that transmits UV and absorbs at least a part of light other than UV. The sample case 63 of FIG. 6 functions as a kind of optical filter by using the dye-dispersed UV-transparent resin 67.69 (an example of the “dye-containing ultraviolet-transmissive resin” described in the present application). That is, it is possible to impart optical filter performance to the sample case.
図８は、本発明のサンプルケース（本願請求項記載の「光学セル」の一例）の断面図であり、（ａ）はキュベット形状のサンプルケース７１、（ｂ）はＰＣＲ管形状のサンプルケース７３である。図９は、図８（ａ）のキュベット形状のサンプルケース７１と吸光度計３からなる光学測定システム７５（本願請求項記載の「光学測定システム」の一例）の断面図である。図８において、３５〜４１、６７及び６９の構成は図６と同様であるが、色素分散ＵＶ透過性樹脂６７・６９の周囲に色素拡散防止部材７７・７９（本願請求項記載の「色素拡散防止部材」の一例）をさらに備えている。色素拡散防止部材７７・７９により、顔料含有樹脂製筐体２１、第１透明樹脂製導光路１５又は第２透明樹脂製導光路１９への色素分散ＵＶ透過性樹脂６７・６９中の色素の拡散を抑制できる。 FIG. 8 is a cross-sectional view of the sample case of the present invention (an example of the “optical cell” described in the present claim), (a) is a cuvette-shaped sample case 71, and (b) is a PCR tube-shaped sample case 73. Is. FIG. 9 is a cross-sectional view of an optical measurement system 75 (an example of the “optical measurement system” described in the claims of the present application) including the cuvette-shaped sample case 71 and the absorbance meter 3 of FIG. 8 (a). In FIG. 8, the configurations of 35 to 41, 67 and 69 are the same as those in FIG. 6, but the dye diffusion preventing member 77.79 (“dye diffusion” described in the present claim is described around the dye-dispersed UV-permeable resin 67/69. An example of a "prevention member") is further provided. Diffusion of the dye in the dye-dispersed UV-transparent resin 67/69 into the pigment-containing resin housing 21, the first transparent resin light guide path 15 or the second transparent resin light guide path 19 by the pigment diffusion prevention member 77/79. Can be suppressed.
図１０は、本発明のサンプルケース８１（本願請求項記載の「光学セル」の一例）の図であり、（ａ）は平面図、（ｂ）はＡ−Ａ断面図、（ｃ）はＢ方向から見たときの側面図である。図１０のサンプルケース８１には、試料８３が流れる試料保持流路８５がある。 10A and 10B are views of a sample case 81 of the present invention (an example of the “optical cell” described in the present claim), (a) is a plan view, (b) is a sectional view taken along the line AA, and FIG. It is a side view when viewed from a direction. The sample case 81 of FIG. 10 has a sample holding flow path 85 through which the sample 83 flows.
例えば、試料保持流路８５につながる試料流入路８７、流出路８９は、試料が流れる方向に垂直な断面の形状が半円形のカマボコ状流路であり、断面の半円の半径は０．５ｍｍである。試料保持流路８５は、円筒形であり、直径８８は１ｍｍ（φ１）、光路長９０は５ｍｍである。よって、試料保持流路８５の体積は、０．５×０．５×π×５≒３．９２５≒４ｍｍ３（＝４μｌ）である。空気との接触面積は、試料導入口９１（φ１の半円）＋試料導出口９３（φ１の半円）＝φ１の円の面積（０．５×０．５×π≒０．７８５ｍｍ２）である。図中の矢印は、光の進行方向を示している。このような構成であれば、十分な光路を確保しながら、空気との接触面積を小さくすることが可能である。 For example, the sample inflow path 87 and the outflow path 89 connected to the sample holding flow path 85 are semicircular turtle-shaped flow paths perpendicular to the direction in which the sample flows, and the radius of the semicircle of the cross section is 0.5 mm. Is. The sample holding flow path 85 has a cylindrical shape, the diameter 88 is 1 mm (φ1), and the optical path length 90 is 5 mm. Therefore, the volume of the sample holding flow path 85 is 0.5 × 0.5 × π × 5 ≈ 3.925 ≈ 4 mm 3 (= 4 μl). The contact area with air is the area of the sample introduction port 91 (φ1 semicircle) + sample outlet 93 (φ1 semicircle) = φ1 circle (0.5 × 0.5 × π ≒ 0.785 mm 2 ). Is. The arrows in the figure indicate the traveling direction of light. With such a configuration, it is possible to reduce the contact area with air while securing a sufficient optical path.
また、試料保持流路８５の体積が５ｍｍ３（＝５μｌ）となる構成としては、下記の構成例１又は２が一例として考えられる。
（構成例１）
試料保持流路の半径：０．５６ｍｍ
試料保持流路のＢ方向から見たときの断面積：０．５６×０．５６×π≒１ｍｍ３
光路長：５ｍｍ
（構成例２）
試料保持流路の半径：０．９ｍｍ
試料保持流路のＢ方向から見たときの断面積：０．９×０．９×π≒２．５ｍｍ３
光路長：２ｍｍ
Further, as a configuration in which the volume of the sample holding flow path 85 is 5 mm 3 (= 5 μl), the following configuration example 1 or 2 can be considered as an example.
(Configuration Example 1)
Radius of sample holding flow path: 0.56 mm
Cross-sectional area when viewed from the B direction of the sample holding flow path: 0.56 × 0.56 × π ≈ 1 mm 3
Optical path length: 5 mm
(Configuration Example 2)
Radius of sample holding flow path: 0.9 mm
Cross-sectional area when viewed from the B direction of the sample holding flow path: 0.9 × 0.9 × π ≈ 2.5 mm 3
Optical path length: 2 mm
上記の構成例２では、試料導入口９１と試料導出口９３は、半径が０．９ｍｍの半円からなるため、試料導入口９１と試料導出口９３を隔てる部分９４は０．２ｍｍであり、これ以上は隔てる部分９４を薄くすることは困難である。よって、試料保持流路８５の体積を５ｍｍ３とする場合、導光路は２ｍｍ以上とすることが望ましい。 In the above configuration example 2, since the sample introduction port 91 and the sample outlet 93 are formed of a semicircle having a radius of 0.9 mm, the portion 94 that separates the sample introduction port 91 and the sample outlet 93 is 0.2 mm. It is difficult to make the separating portion 94 thinner than this. Therefore, when the volume of the sample holding flow path 85 is 5 mm 3 , it is desirable that the light guide path is 2 mm or more.
また、光源からの入射光が通る入射面と、光検出器への出射光が通る出射面の２面はＵＶ透過性透明樹脂９５からなる。その２面に接する部分を除いて、試料保持流路８５は、入射した光を吸収する黒色顔料を含有する顔料含有樹脂９７（本願請求項記載の「迷光吸収色素含有樹脂」の一例）で包囲する。このように、試料保持流路８５を紫外線透過性のシリコーン樹脂９５で挟んだＳＯＴ構造であるため、ＳＯＴ技術を用いた光学測定装置と用いれば、接触面において反射・散乱が抑制され、高精度な光学測定が実施できる。 Further, two surfaces, an incident surface through which the incident light from the light source passes and an exit surface through which the emitted light to the photodetector passes, are made of a UV transmissive transparent resin 95. Except for the portion in contact with the two surfaces, the sample holding flow path 85 is surrounded by a pigment-containing resin 97 containing a black pigment that absorbs incident light (an example of the “stray light absorbing dye-containing resin” described in the claims of the present application). To do. As described above, since the sample holding flow path 85 has an SOT structure sandwiched between ultraviolet-transparent silicone resin 95, reflection and scattering are suppressed on the contact surface when used with an optical measuring device using SOT technology, and high accuracy is achieved. Optical measurement can be performed.
なお、試料溜まり部の形状は、上記したように直方体形状が好ましいが、これに限るものではなく、例えば球状に構成することも可能である。
更には、図２、図４、図６、図８に示すサンプルケース３１、５１、７１、８１においては、空気抜き用孔部４１は流路３７に接続されるように設けられているが、これに限るものではなく、例えば図１１に示すように、流路３７を介さずに試料溜まり部３５に接続されるように設けてもよい。
The shape of the sample reservoir is preferably a rectangular parallelepiped shape as described above, but the shape is not limited to this, and for example, a spherical shape can be formed.
Further, in the sample cases 31, 51, 71, and 81 shown in FIGS. 2, 4, 6, and 8, the air vent hole 41 is provided so as to be connected to the flow path 37. For example, as shown in FIG. 11, the sample may be provided so as to be connected to the sample collecting portion 35 without passing through the flow path 37.
１・・・サンプルケース、３・・・吸光度計、５・・・光学測定システム、７・・・光源、９・・・光検出器、１１・・・試料、１３・・・入射光、１４・・・入射部、１５・・・第１透明樹脂製導光路、１６・・・出射部、１７・・・出射光、１９・・・第２透明樹脂製導光路、２１・・・顔料含有樹脂製筐体、３１・・・サンプルケース、３３・・・ＵＶ透過性樹脂、３５・・・試料溜まり部、３７・・・流路、３９・・・試料注入部、４１・・・空気抜き用孔部、４３・・・光学測定システム、５１・・・サンプルケース、５３・・・吸光度計、５５・・・光学測定システム、５７・・・ＵＶ透過性樹脂、６１・・・キュベット形状のサンプルケース、６３・・・ＰＣＲ管形状のサンプルケース、６５・・・光学測定システム、６７・・・色素分散ＵＶ透過性樹脂、６９・・・色素分散ＵＶ透過性樹脂、７１・・・キュベット形状のサンプルケース、７３・・・ＰＣＲ管形状のサンプルケース、７５・・・光学測定システム、７７・・・色素拡散防止部材、７９・・・色素拡散防止部材、８１・・・サンプルケース、８３・・・試料、８５・・・試料保持流路、８７・・・試料流入路、８８・・・直径、８９・・・流出路、９０・・・光路長、９１・・・試料導入口、９３・・・試料導出口、９４・・・隔てる部分、９５・・・ＵＶ透過性透明樹脂、９７・・・顔料含有樹脂、１０１・・・吸光度計、１０３・・・筐体、１０５・・・検体、１０７・・・ＰＣＲ管、１０９・・・導光路、１１１・・・光源、１１３・・・受光センサ、１１５・・・照射光、１１７・・・観測光、１１９・・・吸光度計、１２１・・・導光路 1 ... sample case, 3 ... absorptiometer, 5 ... optical measurement system, 7 ... light source, 9 ... light detector, 11 ... sample, 13 ... incident light, 14 ... Incident part, 15 ... First transparent resin light guide path, 16 ... Exit part, 17 ... Emission light, 19 ... Second transparent resin light guide path, 21 ... Pigment-containing Resin housing, 31 ... sample case, 33 ... UV permeable resin, 35 ... sample reservoir, 37 ... flow path, 39 ... sample injection section, 41 ... for air bleeding Hole, 43 ... Optical measurement system, 51 ... Sample case, 53 ... Absorber, 55 ... Optical measurement system, 57 ... UV permeable resin, 61 ... Cuvet-shaped sample Case, 63 ... PCR tube-shaped sample case, 65 ... Optical measurement system, 67 ... Dye-dispersed UV-transparent resin, 69 ... Dye-dispersed UV-transparent resin, 71 ... Cuvet-shaped Sample case, 73 ... PCR tube-shaped sample case, 75 ... Optical measurement system, 77 ... Dye diffusion prevention member, 79 ... Dye diffusion prevention member, 81 ... Sample case, 83 ... -Sample, 85 ... sample holding flow path, 87 ... sample inflow path, 88 ... diameter, 89 ... outflow path, 90 ... optical path length, 91 ... sample inlet, 93. .. Sample outlet, 94 ... Separation part, 95 ... UV transparent transparent resin, 97 ... Pigment-containing resin, 101 ... Absorber, 103 ... Housing, 105 ... Specimen , 107 ... PCR tube, 109 ... light guide path, 111 ... light source, 113 ... light receiving sensor, 115 ... irradiation light, 117 ... observation light, 119 ... absorptiometer, 121・ ・ ・ Light guide
Claims (3)
前記光学測定装置は、
前記試料に入射光を入射させる光源部と、
前記試料からの出射光を測定する光検出部と、
前記光源部から前記光学セルへの前記入射光を導光する第１導光路部と、
前記光学セルから前記光検出部への前記出射光を導光する第２導光路部とを有し、
前記第１導光路部および第２導光路部は、それぞれシリコーン樹脂からなり、
前記光学セルは、
前記光学測定装置に対して上方から挿入可能であって、
光学測定対象の試料を保持するための中空部と、
前記中空部を取り囲む周壁部の一部に、
前記入射光が透過する入射部と、
前記出射光が透過する出射部とを有し、
前記入射部と前記出射部が、紫外線透過性樹脂からなる、光学測定システム。 An optical measurement system consisting of an optical measuring device and an optical cell that performs optical measurement of a sample.
The optical measuring device is
A light source unit that causes incident light to enter the sample,
A photodetector that measures the emitted light from the sample,
A first light guide path portion that guides the incident light from the light source portion to the optical cell, and
It has a second light guide path portion that guides the emitted light from the optical cell to the photodetector.
The first light guide path portion and the second light guide path portion are each made of silicone resin.
The optical cell is
It can be inserted from above into the optical measuring device and
A hollow part for holding the sample to be measured optically,
In a part of the peripheral wall portion surrounding the hollow portion,
An incident part through which the incident light is transmitted and
It has an emitting portion through which the emitted light is transmitted, and has an emitting portion.
An optical measurement system in which the incident portion and the outgoing portion are made of an ultraviolet transmissive resin.
それぞれの一部が接する接触面を有し、
屈折率が同一の紫外線透過性樹脂からなり、
前記出射部と前記第２導光路部は、
それぞれの一部が接する接触面を有し、
屈折率が同一の紫外線透過性樹脂からなる、請求項１記載の光学測定システム。 The first light guide path portion and the incident portion are
Each has a contact surface that is in contact with
Made of UV-permeable resin with the same refractive index,
The exit portion and the second light guide path portion
Each has a contact surface that is in contact with
The optical measurement system according to claim 1, further comprising an ultraviolet transmissive resin having the same refractive index.
前記試料に入射光を入射させる光源部と、A light source unit that causes incident light to enter the sample,
前記試料からの出射光を測定する光検出部と、 A photodetector that measures the emitted light from the sample,
前記光源部から前記光学セルへの前記入射光を導光する第１導光路部と、 A first light guide path portion that guides the incident light from the light source portion to the optical cell, and
前記光学セルから前記光検出部への前記出射光を導光する第２導光路部と、 A second light guide path portion that guides the emitted light from the optical cell to the photodetector portion,
前記第１導光路部および前記第２導光路部の間に設けられ、前記光学測定装置に対して、前記光学セルを上方から挿入させる光学セル挿入部とを有し、It is provided between the first light guide path portion and the second light guide path portion, and has an optical cell insertion portion for inserting the optical cell from above into the optical measuring device.
前記第１導光路部および前記第２導光路部は、それぞれシリコーン樹脂からなる、光学測定装置。The first light guide path portion and the second light guide path portion are optical measuring devices each made of a silicone resin.
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| WO2020054561A1 (en) * | 2018-09-11 | 2020-03-19 | ウシオ電機株式会社 | Microplate reader |
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| WO2008133281A1 (en) * | 2007-04-24 | 2008-11-06 | Canon Semiconductor Equipment Inc. | Concentration measuring method and system using refractivity distribution in flowing state |
| JP2010169672A (en) * | 2008-12-22 | 2010-08-05 | Tosoh Corp | Vessel for luminescence measurement |
| US8664617B2 (en) * | 2009-10-08 | 2014-03-04 | Ge Healthcare Uk Limited | Multi-stream spectrophotometer module |
| CN104024895B (en) * | 2011-11-15 | 2017-09-22 | 英派尔科技开发有限公司 | Integrated optical sensor |
| JP6503658B2 (en) * | 2014-08-08 | 2019-04-24 | 国立大学法人九州大学 | Optical analysis system, sensor device and optical analysis method |
| CN115046974A (en) * | 2014-11-25 | 2022-09-13 | 思拓凡瑞典有限公司 | Incorporation of fluorescence detection capability into optical absorbance measurement devices |
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